Tissue culture constant-temperature incubator for herbaceous plants

Abstract

The utility model relates to the technical field of plant tissue culture, in particular to a tissue culture constant-temperature incubator for herbaceous plants, which comprises an annular base and a circular plate rotationally arranged in the annular base, and a rotating mechanism for manually driving the circular plate to rotate is arranged at the bottom of the circular plate. A plurality of placing assemblies which are uniformly distributed along the circumference, are used for placing culture media and can be manually rotated are arranged on the circular plate in a penetrating manner; a hemispherical transparent cover is fixedly arranged at the top of the annular base, a pick-and-place opening is formed in the hemispherical transparent cover, a cover plate used for sealing the pick-and-place opening is arranged on the surface of the hemispherical transparent cover in a sliding mode, and a center cylinder is integrally formed in the center of the hemispherical transparent cover; and a pull-out separation assembly for separating the space at the pick-and-place opening from the rest space in the hemispherical transparent cover is arranged in the central cylinder. According to the constant-temperature incubator, the culture dishes are convenient to take and place, and when the culture dishes are taken and placed, excessive influence of external temperature on internal temperature of the incubator can be avoided.

Description

Technical Field

[0001] The utility model belongs to the technical field of plant tissue culture, in particular to a constant temperature incubator for tissue culture of herbaceous plants. Background Art

[0002] Herbaceous plant tissue culture is the use of herbaceous plant tissues, organs or cells for in vitro culture, and continuous culture under artificially controlled environment through sterile operation to obtain regenerated plants or produce other products with economic value. It is widely used in plant reproduction, breeding and genetic engineering.

[0003] The purpose of the tissue culture experiment of Glechoma longituba is to culture Glechoma longituba in vitro under an artificially controlled environment through aseptic operation, so as to study its growth characteristics and reproductive ability, and provide basic materials for subsequent genetic engineering or breeding work. In the tissue culture experiment of Glechoma longituba, the use of a constant temperature incubator is to provide a stable temperature environment to promote the healthy growth and reproduction of Glechoma longituba tissue culture seedlings. The existing constant temperature incubator is not convenient for taking and placing Glechoma longituba culture dishes, and the incubator needs to be fully opened when taking and placing, causing the external temperature to have too much influence on the internal temperature of the incubator; at the same time, it is difficult to observe the internal Glechoma longituba tissue culture seedlings during the tissue culture process of Glechoma longituba in the existing constant temperature incubator. The experimenters need to frequently open the incubator to check the growth of the Glechoma longituba tissue culture seedlings, which has a great impact on the internal temperature of the incubator.

[0004] To this end, we provide a constant temperature incubator for tissue culture of herbaceous plants to solve the above problems. Summary of the invention

[0005] The utility model aims to provide a constant temperature incubator for tissue culture of herbaceous plants in view of the problems of the background technology.

[0006] The utility model achieves the above-mentioned purpose through the following technical solutions:

[0007] A constant temperature incubator for tissue culture of herbaceous plants comprises an annular base and a circular plate rotatably arranged inside the annular base, a rotating mechanism for manually driving the circular plate to rotate is arranged at the bottom of the circular plate, and a plurality of placement components evenly distributed along the circumference for placing culture medium and which can be rotated manually are arranged through the circular plate.

[0008] A hemispherical transparent cover is fixedly provided on the top of the annular base, a take-in and put-out opening is opened on the hemispherical transparent cover, a cover plate for closing the take-in and put-out opening is slidably provided on the surface of the hemispherical transparent cover, a central tube is integrally formed at the center of the hemispherical transparent cover; a pull-out partition component for separating the space at the take-in and put-out opening from the rest of the space in the hemispherical transparent cover is provided inside the central tube.

[0009] As a further optimization scheme of the utility model, the placement assembly includes a placement plate located in the groove at the top of the circular plate and a second bearing seat fixed at the bottom of the circular plate; the rotating shaft of the placement plate passes through the second bearing seat and a second rotating plate is fixed at the bottom end of the rotating shaft.

[0010] As a further optimization scheme of the utility model, the rotating mechanism includes a gear ring fixed to the bottom of the circular plate and a first bearing seat fixed to the bottom of the annular base; a gear meshing with the gear ring is penetrated through the first bearing seat, and a first turntable is fixed to the bottom end of the rotating shaft of the gear.

[0011] As a further optimization solution of the utility model, a plurality of supporting legs evenly distributed along the circumference are fixedly provided on the bottom of the annular base.

[0012] As a further optimization solution of the utility model, a plurality of ventilation holes evenly distributed in a dot matrix are provided on the surface of the central tube, and a temperature sensor is installed on the upper end of the surface of the central tube.

[0013] As a further optimization scheme of the utility model, the partition assembly includes a partition frame and a pull rod fixed on the partition frame; the pull rod movably passes through the hemispherical transparent cover and has a pull ring and a sealing block at its outer end, and a groove that is snap-fitted with the sealing block is provided on the surface of the hemispherical transparent cover; the partition frame is composed of two side panels and an arc-shaped plate fixed between the two side panels, the arc-shaped plate is located in the central tube and is used to close the ventilation hole, the side panel movably passes through the central tube and its contour matches the hemispherical transparent cover.

[0014] As a further optimization scheme of the utility model, the hemispherical transparent cover is also penetrated by a magnifying component for assisting in observing the tissue culture seedlings in the culture medium, and the magnifying component includes a ball seat penetrated and fixed on the hemispherical transparent cover and a ball head connected to the ball seat; a cylinder body slidingly matched with the ball head is penetrated in the ball head, and a magnifying glass is arranged in the cylinder body.

[0015] As a further optimization scheme of the utility model, a temperature control component is provided on the top of the central tube, and the temperature control component includes an air collecting hood fixedly connected to the central tube; an exhaust pipe, a hot air input pipe and a cold air input pipe are respectively inserted into the air collecting hood.

[0016] The beneficial effects of the utility model are:

[0017] 1. The utility model can rotate the melon seed gold culture dishes to be taken out to the access port in sequence by arranging a circular plate, a rotating mechanism and a partition component, so as to facilitate taking and placing. When taking and placing, the space at the access port is separated from the rest of the space in the hemispherical transparent cover by the partition component, so as to avoid excessive influence of the external temperature on the internal temperature of the incubator.

[0018] 2. The utility model provides a circular plate, an amplifying component and a rotatable placement component, and uses the amplifying component to improve the observation effect, so as to facilitate the observation of the internal melon seed gold tissue culture seedlings. At the same time, the placement component is controlled to rotate during observation to facilitate comprehensive observation of various parts of the melon seed gold tissue culture seedlings, thereby avoiding the problem that the experimenter needs to frequently open the incubator to check the growth of the melon seed gold tissue culture seedlings, which causes a greater impact on the internal environment of the incubator. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a top view of the overall structure of the utility model;

[0020] Figure 2 It is a bottom-up stereoscopic schematic diagram of the overall structure of the utility model;

[0021] Figure 3 This is a schematic diagram of the connection structure between the annular base and the circular plate of the utility model;

[0022] Figure 4 It is a schematic diagram of the placement component and the rotating mechanism structure of the utility model;

[0023] Figure 5 This is a schematic diagram of the internal structure of the hemispherical transparent cover of the utility model;

[0024] Figure 6 This is a schematic diagram of the structure of the partition assembly of the utility model;

[0025] Figure 7 It is a schematic diagram of the structure of the amplifying component of the utility model.

[0026] In the figure:

[0027] 1. Ring base; 101. Support leg; 2. Round plate; 3. Placement assembly; 301. Placement plate; 302. Second bearing seat; 303. Second turntable; 4. Rotation mechanism; 401. Gear ring; 402. Gear; 403. First bearing seat; 404. First turntable; 5. Hemispherical transparent cover; 501. Access port; 502. Cover plate; 503. Center tube; 504. Ventilation hole; 6. Partition assembly; 601. Partition frame; 602. Pull rod; 603. Pull ring; 604. Sealing block; 7. Magnification assembly; 701. Ball seat; 702. Ball head; 703. Cylinder body; 704. Magnifying glass; 8. Temperature control assembly; 801. Air collecting hood; 802. Exhaust pipe; 803. Hot air inlet pipe; 804. Cold air inlet pipe. DETAILED DESCRIPTION

[0028] The present application is further described in detail below in conjunction with the accompanying drawings. It is necessary to point out here that the following specific implementation methods are only used to further illustrate the present application and cannot be understood as limiting the scope of protection of the present application. Technical personnel in this field can make some non-essential improvements and adjustments to the present application based on the above application content.

[0029] Embodiment 1

[0030] To solve the problem that it is not convenient to take out and put out the melon seed gold culture dish in the existing constant temperature incubator, and the incubator needs to be fully opened when taking and putting, which causes the external temperature to have too great an impact on the internal temperature of the incubator, please refer to Figure 1-Figure 2 , Figure 5-Figure 6 The utility model provides a constant temperature incubator for tissue culture of herbaceous plants, comprising an annular base 1 and a circular plate 2 rotatably arranged inside the annular base 1, a plurality of supporting legs 101 evenly distributed along the circumference are fixedly arranged at the bottom of the annular base 1, a rotating mechanism 4 for manually driving the rotation of the circular plate 2 is arranged at the bottom, a plurality of placement components 3 evenly distributed along the circumference for placing melon seed gold culture medium are penetrated through the circular plate 2, a hemispherical transparent cover 5 is fixedly arranged on the top of the annular base 1, a take-in and put-out port 501 is opened on the hemispherical transparent cover 5, a cover plate 502 for closing the take-in and put-out port 501 is slidably arranged on the surface of the hemispherical transparent cover 5, a central tube 503 is integrally formed at the center of the hemispherical transparent cover 5; a pull-out partition component 6 for separating the space at the take-in and put-out port 501 from the rest of the space in the hemispherical transparent cover 5 is arranged inside the central tube 503. The circular plate 2 can be manually driven to rotate through the rotating mechanism 4, so that the melon seed gold culture dish to be taken out can be rotated to the access port 501 for easy access. When taking and placing, the partition component 6 is pulled outward to separate the space at the access port 501 from the rest of the space in the hemispherical transparent cover 5 to prevent the external temperature from having an excessive impact on the internal temperature of the incubator.

[0031] like Figure 3-Figure 4 As shown, the rotating mechanism 4 includes a gear ring 401 fixed to the bottom of the circular plate 2 and a first bearing seat 403 fixed to the bottom of the annular base 1; a gear 402 meshing with the gear ring 401 is provided through the first bearing seat 403, and a first rotating disk 404 is fixed to the bottom end of the rotating shaft of the gear 402. The first rotating disk 404 is manually rotated, and the first rotating disk 404 drives the gear 402 to rotate, the gear 402 drives the gear ring 401 to rotate, and the gear ring 401 drives the circular plate 2 to rotate, and the circular plate 2 rotates the melon seed gold culture dish to be taken out to the taking and releasing port 501.

[0032] like Figure 6As shown, the partition assembly 6 includes a partition frame 601 and a pull rod 602 fixed on the partition frame 601; the pull rod 602 movably passes through the hemispherical transparent cover 5 and has a pull ring 603 and a sealing block 604 at its outer end, and a groove that is snap-fitted with the sealing block 604 is provided on the surface of the hemispherical transparent cover 5; the partition frame 601 is composed of two side panels and an arc-shaped plate fixed between the two side panels, the arc-shaped plate is located in the central tube 503 and is used to close the ventilation hole 504, the side panel movably passes through the central tube 503 and its contour matches the hemispherical transparent cover 5. When in use, the pull rod 602 is pulled outward through the pull ring 603, and the pull rod 602 drives the partition frame 601 to move outward until the arc plate abuts against the inner wall of the central tube 503, thereby closing the ventilation hole 504. At this time, the side plate abuts against the hemispherical transparent cover 5, and the melon seed gold culture dish to be taken out is separated from the other melon seed gold culture dishes by the partition frame 601; after the melon seed gold culture dish is taken out and placed, the pull rod 602 is pushed inward until the sealing block 604 is stuck in the groove on the surface of the hemispherical transparent cover 5.

[0033] Embodiment 2

[0034] On the basis of the first embodiment, in order to solve the problem that the temperature uniformity of the existing constant temperature incubator is insufficient and the temperature control rate is low, as Figure 1 , Figure 5 As shown, a plurality of ventilation holes 504 evenly distributed in a dot matrix are provided on the surface of the central tube 503, and a temperature sensor is installed on the upper end of the surface of the central tube 503; a temperature control component 8 is provided on the top of the central tube 503, and the temperature control component 8 includes an air collecting cover 801 fixedly connected to the central tube 503; an air extraction pipe 802, a hot air input pipe 803 and a cold air input pipe 804 are respectively inserted into the air collecting cover 801. When the temperature inside the incubator is too high, part of the high-temperature gas is first extracted through the air extraction pipe 802, and then cold air is input through the cold air input pipe 804, so as to reduce the temperature inside the incubator more quickly, and the cold air can be evenly diffused to various areas of the incubator through the plurality of ventilation holes 504; when the temperature inside the incubator is too low, part of the low-temperature gas is first extracted through the air extraction pipe 802, and then hot air is input through the hot air input pipe 803, so as to increase the temperature inside the incubator more quickly.

[0035] Embodiment 3

[0036] On the basis of the first and second embodiments, in order to solve the problem that it is difficult to observe the internal melon seedlings during the melon seedling tissue culture process in the existing constant temperature incubator, the experimenter needs to frequently open the incubator to check the growth of the melon seedlings, which has a great impact on the internal environment of the incubator. Figure 4 , Figure 7As shown, the placement component 3 can be rotated manually, and the hemispherical transparent cover 5 is also provided with a magnifying component 7 for assisting in observing the tissue culture seedlings in the culture medium. The magnifying component 7 is used to improve the observation effect, and the magnifying component 7 can adjust the orientation and the insertion length to adapt to the use of different specifications of melon seed gold tissue culture seedlings. At the same time, the placement component 3 is controlled to rotate itself during observation to facilitate comprehensive observation of all parts of the melon seed gold tissue culture seedlings.

[0037] The placement assembly 3 includes a placement plate 301 located in the groove at the top of the circular plate 2 and a second bearing seat 302 fixedly arranged at the bottom of the circular plate 2; the rotation axis of the placement plate 301 passes through the second bearing seat 302 and a second rotating plate 303 is fixedly arranged at the bottom of the rotating axis. When in use, the second rotating plate 303 is manually rotated, the second rotating plate 303 drives the placement plate 301 to rotate, and the placement plate 301 drives the culture dish therein to rotate.

[0038] The magnifying assembly 7 includes a ball seat 701 that penetrates and is fixed on the hemispherical transparent cover 5, and a ball head 702 that is connected to the ball seat 701; a cylinder 703 that is slidably matched with the ball head 702 is penetrated in the ball head 702, and a magnifying glass 704 is arranged in the cylinder 703. When in use, based on actual needs, the orientation of the cylinder 703 is adjusted through the cooperation between the ball head 702 and the ball seat 701, and the extension length of the cylinder 703 is adjusted by pushing and pulling the cylinder 703 to make it move along the ball head 702.

[0039] The above-mentioned embodiment only expresses one implementation method of the utility model, and its description is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the utility model. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the utility model, which all belong to the protection scope of the utility model.

Claims

1. A constant temperature incubator for tissue culture of herbaceous plants, comprising an annular base (1) and a circular plate (2) rotatably arranged inside the annular base (1), characterized in that: The bottom of the circular plate (2) is provided with a rotating mechanism (4) for manually driving the circular plate (2) to rotate, and the circular plate (2) is penetrated by a plurality of placement components (3) evenly distributed along the circumference for placing culture media and capable of manual rotation. A hemispherical transparent cover (5) is fixedly provided on the top of the annular base (1), a taking-in and putting-out opening (501) is provided on the hemispherical transparent cover (5), a cover plate (502) for closing the taking-in and putting-out opening (501) is slidably provided on the surface of the hemispherical transparent cover (5), and a central tube (503) is integrally formed at the center of the hemispherical transparent cover (5); The interior of the central tube (503) is provided with a pull-out partition assembly (6) for separating the space at the access opening (501) from the remaining space in the hemispherical transparent cover (5).

2. A constant temperature incubator for tissue culture of herbaceous plants according to claim 1, characterized in that: The placement assembly (3) comprises a placement plate (301) located in a groove at the top of the circular plate (2) and a second bearing seat (302) fixedly arranged at the bottom of the circular plate (2); The rotating shaft of the placement plate (301) passes through the second bearing seat (302), and a second rotating plate (303) is fixedly arranged at the bottom end of the rotating shaft.

3. The constant temperature incubator for tissue culture of herbaceous plants according to claim 1, characterized in that: The rotating mechanism (4) comprises a gear ring (401) fixedly arranged at the bottom of the circular plate (2) and a first bearing seat (403) fixedly arranged at the bottom of the annular base (1); A gear (402) meshing with the gear ring (401) is provided through the first bearing seat (403), and a first rotating disk (404) is fixedly provided at the bottom end of the rotating shaft of the gear (402).

4. The constant temperature incubator for tissue culture of herbaceous plants according to claim 1, characterized in that: A plurality of support legs (101) evenly distributed along the circumference are fixedly provided on the bottom of the annular base (1).

5. The constant temperature incubator for tissue culture of herbaceous plants according to claim 1, characterized in that: A plurality of ventilation holes (504) evenly distributed in a dot matrix are provided on the surface of the central tube (503), and a temperature sensor is installed at the upper end of the surface of the central tube (503).

6. A constant temperature incubator for tissue culture of herbaceous plants according to claim 5, characterized in that: The partition assembly (6) comprises a partition frame (601) and a pull rod (602) fixed on the partition frame (601); The pull rod (602) movably passes through the hemispherical transparent cover (5) and is provided with a pull ring (603) and a sealing block (604) at its outer end. A groove for engaging with the sealing block (604) is provided on the surface of the hemispherical transparent cover (5). The partition frame (601) is composed of two side plates and an arc plate fixed between the two side plates. The arc plate is located in the central tube (503) and is used to close the ventilation hole (504). The side plate movably passes through the central tube (503) and its contour matches the hemispherical transparent cover (5).

7. The constant temperature incubator for tissue culture of herbaceous plants according to claim 1, characterized in that: The hemispherical transparent cover (5) is also provided with an amplifying component (7) for assisting in observing the tissue culture seedlings in the culture medium, and the amplifying component (7) comprises a ball seat (701) fixedly passing through the hemispherical transparent cover (5) and a ball head (702) connected to the ball seat (701); A cylinder (703) is provided through the ball head (702) and is slidably matched with the ball head (702). A magnifying glass (704) is provided in the cylinder (703).

8. The constant temperature incubator for tissue culture of herbaceous plants according to claim 1, characterized in that: A temperature control component (8) is provided on the top of the central tube (503), and the temperature control component (8) includes an air collecting hood (801) fixedly connected to the central tube (503); An exhaust pipe (802), a hot air input pipe (803) and a cold air input pipe (804) are respectively inserted into the air collecting hood (801).

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