Separation, purification and sedimentation device for chlorella polypeptide

Abstract

The utility model discloses a chlorella polypeptide separating, purifying and settling device, and relates to the technical field of biological extraction. The utility model provides a chlorella polypeptide separating, purifying and settling device which comprises a supporting base, a settling box fixedly connected with the top end of the supporting base, a slag outlet pipe communicated with the bottom end of the settling box and two liquid outlet pipes communicated with the settling box, and further comprises guide plates distributed at equal intervals, and the guide plates distributed at equal intervals are fixedly connected into the settling box. And yarn film layers which are distributed at equal intervals are fixedly connected in the settling box. Opening the isolation plates, adding an enzymatic hydrolysate of chlorella and water into the settling box together for dilution and settling, guiding the solution through a yarn membrane layer and a guide plate when the solution enters the settling box and intercepting chlorella cell debris, after settling for a period of time, controlling the upper isolation plate to be closed, then pumping out an upper clear polypeptide liquid, and then discharging the upper clear polypeptide liquid; and after the enzymatic hydrolysate of chlorella in the settling box is settled, the isolation plate on the lower layer is controlled to be closed, and then the residual polypeptide liquid is pumped out.

Description

Technical Field

[0001] The utility model relates to the technical field of biological extraction, in particular to a separation, purification and sedimentation device for chlorella polypeptides. Background Art

[0002] Chlorella peptides refer to peptide compounds obtained by enzymatic hydrolysis of proteins extracted from Chlorella. Chlorella is a single-cell green algae that is rich in protein, vitamins, minerals and antioxidants, and has high nutritional value and multiple health benefits. The separation and purification of Chlorella peptides is a complex process involving a variety of technologies, one of which is sedimentation technology.

[0003] At present, the commonly used methods for treating Chlorella hydrolysate on the market mostly use a single sedimentation tank for sedimentation treatment. Although this method can initially achieve solid-liquid separation, it has the following significant disadvantages: low sedimentation efficiency, different sedimentation rates of Chlorella cell fragments and macromolecules in a single sedimentation tank, and the need to wait for the solution to completely settle before separating the polypeptide liquid, resulting in a long overall sedimentation time, affecting production efficiency; poor separation effect, due to the lack of effective classification in the sedimentation process, the final product contains more tiny suspended matter, affecting the purity and quality of the product.

[0004] In order to solve the above problems, we propose a separation and purification sedimentation device for Chlorella polypeptides. The sedimentation device improves the sedimentation efficiency and purity by setting a multi-layer sedimentation structure. Utility Model Content

[0005] In order to overcome the shortcomings of low sedimentation efficiency and poor separation effect of a single sedimentation tank, the utility model provides a separation and purification sedimentation device for Chlorella polypeptides. The sedimentation device improves sedimentation efficiency and purity by arranging a multi-layer sedimentation structure.

[0006] The technical implementation scheme of the utility model is: a separation and purification sedimentation device for Chlorella polypeptides, including a supporting base, a sedimentation box fixedly connected to the top of the supporting base, a slag discharge pipe connected to the bottom of the sedimentation box, two liquid discharge pipes connected to the sedimentation box, and also including equidistantly distributed guide plates, equidistantly distributed guide plates fixedly connected in the sedimentation box, equidistantly distributed gauze film layers fixedly connected in the sedimentation box, two rows of equidistantly distributed isolation plates rotatably connected in the sedimentation box, one side of two adjacent isolation plates close to each other is an arc structure that fits each other, longitudinally symmetrical torsion springs are connected between the isolation plates and the sedimentation box, a pushing component for controlling the opening and closing of the isolation plates is provided on the sedimentation box, and a locking component for locking the pushing component is provided on the sedimentation box.

[0007] In a preferred embodiment of the utility model, the guide plate is arranged at an angle, and the surface of the guide plate is provided with equidistantly distributed interception strips for intercepting Chlorella cell fragments.

[0008] In a preferred embodiment of the utility model, the pushing assembly includes a contact block, which is fixedly connected to the isolation plate, a guide rod is fixedly connected to the sedimentation box, a pushing frame is slidably connected to the guide rod, and the pushing frame is provided with equidistantly distributed protrusions, and the protrusions of the pushing frame are squeezed and matched with the contact block.

[0009] In a preferred embodiment of the present utility model, a handle is fixedly connected to the pushing frame.

[0010] In a preferred embodiment of the utility model, the locking assembly includes a fixed block, the fixed block is fixedly connected to the sedimentation box, a locking rod is slidably connected to the fixed block, and an elastic member is connected between the locking rod and the fixed block.

[0011] In a preferred embodiment of the utility model, the bottom end of the locking rod is inclined, and a locking groove adapted to the locking rod is opened on the pushing frame.

[0012] Compared with the prior art, the utility model has the following advantages: the isolation plate is opened, and the enzymatic hydrolyzate of Chlorella and water are added into the sedimentation box together for dilution and sedimentation. When the diluted solution enters the sedimentation box, it will be guided by the inclined guide plate and intercept the Chlorella cell fragments and adsorbed floating objects and suspended large particles. After sedimentation for a period of time, the uppermost solution has been clarified, the upper isolation plate is controlled to be closed, and then the clarified polypeptide liquid in the upper layer is extracted. When the enzymatic hydrolyzate of Chlorella in the sedimentation box has completed sedimentation, the lower isolation plate is controlled to be closed, and the macromolecular substances are isolated at the bottom of the sedimentation box, and then the remaining polypeptide liquid is extracted. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a schematic diagram of the three-dimensional structure of the utility model from the first viewing angle.

[0014] Figure 2 It is a schematic diagram of the three-dimensional structure of the utility model from a second viewing angle.

[0015] Figure 3 It is a cross-sectional structural schematic diagram of the utility model.

[0016] Figure 4 It is a three-dimensional structural schematic diagram of the guide plate of the utility model.

[0017] Figure 5 It is a three-dimensional structural schematic diagram of the isolation plate, torsion spring and contact block of the utility model.

[0018] Figure 6 It is a three-dimensional structural schematic diagram of the contact block, guide rod, push frame and other components of the utility model.

[0019] Figure 7 It is a three-dimensional structural schematic diagram of the fixing block, the locking rod, the elastic member and other components of the utility model.

[0020] The markings of the components in the accompanying drawings are as follows: 1. Support base, 2. Settling box, 3. Slag discharge pipe, 4. Liquid discharge pipe, 5. Guide plate, 51. Gauze film layer, 6. Isolation plate, 7. Torsion spring, 8. Contact block, 9. Guide rod, 10. Push frame, 11. Fixed block, 12. Positioning rod, 13. Elastic part. DETAILED DESCRIPTION

[0021] Although the present invention may be described with respect to a specific application or industry, those skilled in the art will recognize the broader applicability of the present invention. Those of ordinary skill in the art will recognize that terms such as: above, below, upward, downward, etc. are used to describe the drawings and do not represent limitations on the scope of the present invention as defined by the appended claims. Any numerical designations such as: first or second are merely exemplary and are not intended to limit the scope of the present invention in any way.

[0022] Example 1: A separation and purification sedimentation device for Chlorella polypeptides, such as Figure 1-Figure 7 As shown, it includes a support base 1, a sedimentation box 2, a slag discharge pipe 3, a liquid discharge pipe 4, a guide plate 5, a gauze film layer 51, an isolation plate 6, a torsion spring 7, a pushing component and a locking component. The upper side of the support base 1 is fixedly connected to the sedimentation box 2, the lower side of the sedimentation box 2 is connected to the slag discharge pipe 3, the left side of the sedimentation box 2 is connected to two liquid discharge pipes 4, five equidistantly distributed guide plates 5 are fixedly connected to the upper part of the sedimentation box 2, five equidistantly distributed gauze film layers 51 are fixedly connected to the upper part of the sedimentation box 2, and the gauze film layer 51 and the guide plate 51 are fixedly connected to the upper part of the sedimentation box 2. The plate 5 is arranged obliquely, and the surface of the guide plate 5 is provided with equidistantly distributed interception strips for intercepting Chlorella cell fragments. A row of equidistantly distributed isolation plates 6 are rotatably connected to the middle and lower parts of the sedimentation box 2. The sides of two adjacent isolation plates 6 close to each other are arc structures that fit each other. Torsion springs 7 are connected between the front and rear ends of the isolation plates 6 and the sedimentation box 2. The sedimentation box 2 is provided with a pushing component for controlling the opening and closing of the isolation plates 6, and the sedimentation box 2 is provided with a locking component for locking the pushing component.

[0023] When using the device, the staff first controls the isolation plate 6 to rotate upward and open through the pushing component, and the torsion spring 7 is deformed. Then, the staff locks the pushing component through the locking component, so that the upper and lower isolation plates 6 remain in the open state. Then, the staff adds the enzymatic hydrolysate of Chlorella and water into the sedimentation box 2, and dilutes the enzymatic hydrolysate of Chlorella with water to reduce the viscosity, promote particle dispersion, and increase the sedimentation rate. When the diluted solution enters the sedimentation box 2, it will absorb floating objects and suspended large particles through the gauze film layer 51, and then be guided by the inclined guide plate 5. The interception strips arranged on the surface of the guide plate 5 can intercept Chlorella cell fragments. The diluted solution enters the sedimentation box 2 for sedimentation. After settling for a period of time, the uppermost solution has been clarified. At this time, the staff releases the locking component and the pushing component of the upper layer. Under the action of the torsion spring 7 resetting, the upper isolation plate 6 rotates downward and resets. After the isolation plate 6 rotates downward, the adjacent isolation plates 6 fit together to separate the clarified upper solution and the lower solution that is settling. Then the staff extracts the clarified polypeptide liquid from the upper layer through the upper liquid outlet pipe 4. When the Chlorella hydrolysate in the sedimentation box 2 completes the sedimentation, the staff controls the lower isolation plate 6 to close. At this time, the macromolecular substances are precipitated at the bottom of the sedimentation box 2. When the staff extracts the remaining polypeptide liquid through the lower liquid outlet pipe 4, the lower isolation plate 6 can isolate the algae residue at the bottom of the sedimentation box 2. After the polypeptide liquid is extracted, the precipitated algae residue can be discharged through the residue outlet pipe 3. In this way, multi-layer sedimentation extraction can be carried out, and there is no need to wait for the Chlorella hydrolysate to completely settle before separating the polypeptide liquid, thereby effectively improving the sedimentation efficiency.

[0024] Embodiment 2: Based on embodiment 1, Figure 1 , Figure 2 , Figure 3 , Figure 5 and Figure 6 As shown, the pushing assembly includes a contact block 8, a guide rod 9 and a pushing frame 10. The front and rear parts of the isolation plate 6 are fixedly connected to the contact block 8, the middle and lower parts of the right side of the sedimentation box 2 are fixedly connected to the guide rod 9, the pushing frame 10 is slidably connected to the guide rod 9, and a handle is fixedly connected to the right side of the pushing frame 10. The front and rear parts of the pushing frame 10 are provided with equidistantly distributed protrusions, and the protrusions of the pushing frame 10 are squeezed and fitted with the contact block 8.

[0025] When using the device, the staff first controls the push frame 10 to slide to the left on the guide rod 9. The push frame 10 slides to the left to squeeze the contact block 8 to rotate. The rotation of the contact block 8 drives the isolation plate 6 to rotate upward and open. When it is necessary to extract the polypeptide liquid, the staff controls the push frame 10 to slide to the right and no longer squeezes the contact block 8, and the isolation plate 6 rotates downward and closes.

[0026] like Figure 1 and Figure 7As shown, the locking assembly includes a fixed block 11, a locking rod 12 and an elastic member 13. The fixed block 11 is fixedly connected to the middle and lower parts of the right side of the sedimentation box 2. The locking rod 12 is slidably connected to the fixed block 11. The bottom end of the locking rod 12 is inclined. A slot adapted to the locking rod 12 is opened in the middle of the pushing frame 10, and an elastic member 13 is connected between the locking rod 12 and the fixed block 11.

[0027] When using the device, the pushing frame 10 slides to the left, squeezing the locking rod 12 to slide upward, and the elastic member 13 is deformed. When the slot on the pushing frame 10 moves to below the locking rod 12, the locking rod 12 slides downward and is locked in the slot under the action of elastic reset, thereby fixing the position of the pushing frame 10, so that the isolation plate 6 remains open, so that the macromolecular substances pass through the isolation plate 6 and precipitate downward. When the polypeptide liquid needs to be extracted, the staff controls the locking rod 12 to slide upward to release the pushing frame 10, and the isolation plate 6 will rotate downward and close.

[0028] Although the present disclosure has been described with respect to only a limited number of embodiments, those skilled in the art having benefit of this disclosure will appreciate that various other embodiments may be designed without departing from the scope of the present invention. Therefore, the scope of the present invention should be limited only by the appended claims.

Claims

1. A separation and purification sedimentation device for Chlorella polypeptides, comprising a support base (1), the top of the support base (1) is fixedly connected to a sedimentation box (2), the bottom of the sedimentation box (2) is connected to a slag discharge pipe (3), and the sedimentation box (2) is connected to two liquid discharge pipes (4), wherein: It also includes equally spaced guide plates (5), which are fixedly connected to the inside of the sedimentation box (2), and a gauze film layer (51) is fixedly connected to the inside of the sedimentation box (2), and two rows of equally spaced isolation plates (6) are rotatably connected to the inside of the sedimentation box (2), and the sides of two adjacent isolation plates (6) that are close to each other are circular arc structures that fit each other, and longitudinally symmetrical torsion springs (7) are connected between the isolation plates (6) and the sedimentation box (2), and a pushing component for controlling the opening and closing of the isolation plates (6) is provided on the sedimentation box (2), and a locking component for locking the pushing component is provided on the sedimentation box (2).

2. The separation, purification and sedimentation device for Chlorella polypeptide according to claim 1, characterized in that: The guide plate (5) is arranged at an inclination, and the surface of the guide plate (5) is provided with equidistantly distributed interception strips for intercepting Chlorella cell fragments.

3. The separation, purification and sedimentation device for Chlorella polypeptide according to claim 2, characterized in that: The pushing assembly comprises a contact block (8), the contact block (8) is fixedly connected to the isolation plate (6), a guide rod (9) is fixedly connected to the sedimentation box (2), a pushing frame (10) is slidably connected to the guide rod (9), and the pushing frame (10) is provided with equidistantly distributed protrusions, and the protrusions of the pushing frame (10) are pressed and matched with the contact block (8).

4. The separation, purification and sedimentation device for Chlorella polypeptide according to claim 3, characterized in that: A handle is fixedly connected to the pushing frame (10).

5. The separation, purification and sedimentation device for Chlorella polypeptide according to claim 4, characterized in that: The locking assembly comprises a fixed block (11), the fixed block (11) is fixedly connected to the sedimentation box (2), a locking rod (12) is slidably connected to the fixed block (11), and an elastic member (13) is connected between the locking rod (12) and the fixed block (11).

6. The separation, purification and sedimentation device for Chlorella polypeptide according to claim 5, characterized in that: The bottom end of the locking rod (12) is tilted, and a locking groove adapted to the locking rod (12) is provided on the pushing frame (10).

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