Carbon dioxide control system of bone marrow cell incubator

Abstract

The utility model relates to a carbon dioxide control system of a bone marrow cell incubator, which comprises a carbon dioxide concentration sensor positioned in the incubator; the plurality of carbon dioxide gas tanks are communicated with the incubator; an automatic replacement device; the central controller is electrically connected with the automatic replacement device and the carbon dioxide sensor; the buffer box is located between the incubator and the carbon dioxide gas tank and communicated with the incubator and the carbon dioxide gas tank, and a temperature control unit is arranged in the buffer box. Automatic replacement of the gas tank is achieved, continuous supply of carbon dioxide to the incubator is guaranteed, the situation that the carbon dioxide gas tank is used up at night or in an unattended state, and cell death is caused due to the fact that gas cannot be supplied is reduced, and the purposes that the influence of carbon dioxide supply on the cell culture efficiency is reduced, and interference on experimental results is reduced are achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a carbon dioxide control system of a bone marrow cell culture box. Background Art

[0002] A certain concentration of carbon dioxide needs to be supplied during cell culture to simulate the environment in which cells grow in the body. Carbon dioxide reacts with water to produce carbonic acid, which can adjust the pH value of the culture medium to keep it within the physiological range required for cell growth, helping to maintain cell morphology and functional integrity and promote cell division and proliferation.

[0003] The incubator for bone marrow cells in the laboratory routinely maintains a carbon dioxide concentration of 5%, using carbon dioxide compressed gas cylinders to release gas into the cell incubator. However, the existing cell incubators are insufficient in carbon dioxide supply, especially at night or when unattended. The carbon dioxide cylinders are exhausted and unable to supply gas. The lack of carbon dioxide in the cell culture chamber leads to cell death, which greatly affects the efficiency of cell culture and interferes with experimental results. Utility Model Content

[0004] In view of the deficiencies of the prior art, the purpose of the utility model is to reduce the impact of carbon dioxide supply on cell culture efficiency and reduce interference with experimental results.

[0005] A carbon dioxide control system for a bone marrow cell culture box, connected to the culture box, the control system comprising:

[0006] A carbon dioxide concentration sensor, located inside the incubator;

[0007] A plurality of carbon dioxide gas cylinders are connected to the incubators and are used to sequentially supply carbon dioxide to the incubators;

[0008] An automatic replacement device, located between the multiple carbon dioxide gas cylinders and the incubator, is used to control the multiple carbon dioxide gas cylinders to automatically replace and deflate in sequence;

[0009] The central controller is used to control the deflation of multiple carbon dioxide gas cylinders and the gas supply to the incubator. The central controller is electrically connected to the automatic replacement device and the carbon dioxide sensor.

[0010] The replacement device includes a sensor for detecting whether the carbon dioxide gas cylinder is exhausted and an electric valve for opening and closing the carbon dioxide gas cylinder to release gas. Each carbon dioxide gas cylinder is provided with a sensor and an electric valve, and the central controller is electrically connected to the sensor and the electric valve.

[0011] The replacement device also includes an alarm for reminding that all carbon dioxide gas cylinders are exhausted, and the alarm is electrically connected to the central controller and the sensor.

[0012] The carbon dioxide gas cylinder is also provided with a safety temperature detection component, and the detection component is electrically connected to the alarm component through the central controller.

[0013] The control system also includes a buffer box for pre-storing carbon dioxide, the buffer box is located between the incubator and the plurality of carbon dioxide gas cylinders, and a temperature control unit is provided in the buffer box for adjusting the temperature of the carbon dioxide before it is released into the incubator.

[0014] The temperature control unit comprises a temperature sensor located in the incubator and a temperature regulating member located in the buffer box, and both the temperature sensor and the temperature regulating member are electrically connected to the central controller.

[0015] The temperature regulating member adopts an electric heating plate, and the electric heating plate is fixedly connected to the inner wall of the buffer box.

[0016] Compared with the closest prior art, the utility model has the following beneficial effects:

[0017] 1. Through the setting of carbon dioxide concentration sensor, multiple carbon dioxide gas cylinders, automatic replacement device and central controller, when the carbon dioxide in a gas cylinder is about to run out, it will automatically switch to the next gas cylinder, realizing automatic replacement of gas cylinders, ensuring the continuous supply of carbon dioxide to the incubator, and reducing the situation where the carbon dioxide gas cylinder runs out at night or when no one is on duty, and the inability to supply gas leads to cell death, so as to reduce the impact of carbon dioxide supply on cell culture efficiency and reduce interference with experimental results.

[0018] 2. The temperature of carbon dioxide is pre-regulated by setting up a buffer box and a temperature control unit, thereby avoiding the impact of carbon dioxide release on the temperature inside the incubator. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a structural schematic diagram of the carbon dioxide control system of this application.

[0020] Legend:

[0021] 1. Incubator; 11. Carbon dioxide sensor; 2. Carbon dioxide tank; 21. Multi-way catheter; 3. Automatic replacement device; 31. Sensing element; 32. Electric valve; 4. Buffer box; 41. Temperature sensor; 42. Temperature regulating element. DETAILED DESCRIPTION

[0022] In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present utility model. In addition, the terms "first", "second", etc. are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, features defined as "first", "second", etc. may explicitly or implicitly include one or more of the features. In the description of the present utility model, unless otherwise specified, "multiple" means two or more.

[0023] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood by specific circumstances.

[0024] The present application embodiment discloses a carbon dioxide control system for a bone marrow cell culture chamber. Figure 1 A carbon dioxide control system for a bone marrow cell culture box includes multiple carbon dioxide gas cylinders 2, an automatic replacement device 3, a buffer box 4, a central controller and a carbon dioxide sensor 11. The buffer box 4 is located between the multiple carbon dioxide gas cylinders 2 and the culture box 1 and is connected to both. A temperature control unit is provided in the buffer box 4. The carbon dioxide sensor 11 is fixedly installed on the inner wall of the culture box 1. The carbon dioxide sensor 11, the automatic replacement device 3 and the temperature control unit are all electrically connected to the central controller.

[0025] The carbon dioxide sensor 11 is used to detect the carbon dioxide concentration in the incubator 1 in real time, and the detection signal is transmitted to the central controller. The central controller controls the buffer box 4 to release carbon dioxide to the incubator 1. At the same time, the temperature before the carbon dioxide is released into the incubator 1 is adjusted through the temperature control unit to maintain a constant carbon dioxide concentration and temperature in the incubator 1, thereby improving the stability and efficiency of cell culture; the central controller controls the automatic replacement device 3 to replace different carbon dioxide gas cylinders 2 for deflation. When the carbon dioxide in a gas cylinder is about to run out, it automatically switches to the next gas cylinder, thereby ensuring the continuous supply of carbon dioxide to the incubator 1, reducing the situation where the carbon dioxide gas cylinder 2 runs out at night or when no one is on duty, and cannot supply gas, resulting in cell death, thereby achieving the purpose of reducing the influence of carbon dioxide supply on cell culture efficiency and reducing interference with experimental results.

[0026] Reference Figure 1 , multiple carbon dioxide gas cylinders 2 are placed upright in the same closed storage box, a multi-way conduit 21 is provided between the multiple carbon dioxide gas cylinders 2 and the buffer box 4, the multi-way ends of the multi-way conduit 21 are respectively fixedly connected to the multiple carbon dioxide gas release ports, and one end of the multi-way conduit 21 away from the carbon dioxide gas cylinder 2 is fixedly connected to the buffer box 4; the automatic replacement device 3 includes a sensor 31 for detecting whether the carbon dioxide gas cylinder 2 is exhausted and an electric valve 32 for opening and closing the carbon dioxide gas cylinder 2 to release gas, and the sensor 31 and the electric valve 32 are both provided with multiple, and are connected with The number of carbon dioxide gas cylinders 2 is the same. The sensor 31 can adopt the carbon dioxide sensor 11, which is installed on the inner wall of the gas release port of the carbon dioxide gas cylinder 2. The electric valve 32 can adopt a small electromagnetic valve suitable for gas control. The electric valve 32 is sleeved on the multi-way conduit 21. Each electric valve 32 is fixedly connected and communicated with the multi-way end of the multi-channel pipe. Multiple electric valves 32 are used to control the gas release opening and closing of each carbon dioxide gas cylinder 2 respectively. The sensor 31 and the electric valve 32 are electrically connected to the central controller.

[0027] In the central controller, multiple electric valves 32 and multiple sensors are marked in order in sequence. The central controller first controls the electric valve 32 with the first order to open for deflation. The deflation speed and pressure are controlled during deflation to avoid damage to equipment and personnel caused by excessive deflation speed and pressure. When the sensor in the corresponding carbon dioxide gas cylinder 2 senses that the carbon dioxide in the gas cylinder is about to be exhausted, it sends a signal to the central controller. After receiving the signal, the central controller controls the next carbon dioxide gas cylinder 2 in order to start deflation, thereby realizing the function of automatically replacing multiple carbon dioxide gas cylinders 2 and supplying gas to the incubator 1 in stages.

[0028] When all the carbon dioxide gas in the carbon dioxide gas tanks 2 are exhausted, in order to further reduce the impact on the experimental results, refer to Figure 1An alarm is installed outside the closed box where the carbon dioxide gas cylinder 2 is located. The alarm is electrically connected to the central controller and the sensor 31. When the central controller receives the gas exhaustion signal from all the sensors 31, the alarm is controlled to start sounding, reminding laboratory personnel to replace the carbon dioxide gas cylinder 2 in time.

[0029] In addition, a safety temperature detection device and a safety pressure detection device are also provided for the carbon dioxide gas cylinder 2, and the temperature detection device and the pressure detection device are electrically connected to the alarm through the central controller. When the temperature value and the pressure value of the carbon dioxide gas cylinder 2 exceed the preset safety line, a signal is sent to the central controller to control the alarm to issue an alarm reminder.

[0030] When adjusting the carbon dioxide concentration, the compressed carbon dioxide is released and gasified to do work externally, and the temperature decreases, which will affect the temperature in the incubator 1. Figure 1 The temperature control unit in the buffer box 4 includes a temperature sensor 41 located in the incubator 1 and a temperature regulating member 42 located in the buffer box 4. The temperature sensor 41 monitors the temperature in the incubator 1 in real time. The temperature sensor 41 and the temperature regulating member 42 are electrically connected to the central controller. The temperature regulating member 42 can adopt an electric heating plate, which has fast heating efficiency and is easy to use. When the temperature sensor 41 detects that the temperature in the incubator 1 drops, it sends a signal to the central controller, and the central controller controls the electric heating plate to start heating the carbon dioxide gas in the buffer box 4, thereby increasing the temperature in the incubator 1 and keeping it constant at about 37°C. The heating temperature of the electric heating plate in the incubator 1 is controlled as much as possible not to exceed the critical temperature of carbon dioxide (usually 31.1°C) to prevent the gas from liquefying or generating high pressure.

[0031] The above is a detailed description of an embodiment of the utility model, but the content is only a preferred embodiment of the utility model and cannot be considered to limit the scope of implementation of the utility model. All equivalent changes and improvements made within the scope of application of the utility model should still fall within the scope of the patent coverage of the utility model.

Claims

1. A carbon dioxide control system for a bone marrow cell culture box, connected to a culture box (1), characterized in that: The control system comprises: A carbon dioxide sensor (11), located in the incubator (1), for sensing carbon dioxide concentration; A plurality of carbon dioxide gas cylinders (2) are connected to the incubator (1) and are used to sequentially supply carbon dioxide to the incubator (1); An automatic replacement device (3), located between the plurality of carbon dioxide gas cylinders (2) and the incubator (1), and used for controlling the plurality of carbon dioxide gas cylinders (2) to automatically replace and deflate in sequence; The central controller is used to control the deflation of the plurality of carbon dioxide gas cylinders (2) and the gas supply to the incubator (1). The central controller is electrically connected to the automatic replacement device (3) and the carbon dioxide sensor (11).

2. A carbon dioxide control system for a bone marrow cell culture chamber as claimed in claim 1, characterized in that: The replacement device comprises a sensor (31) for detecting whether the carbon dioxide gas cylinder (2) is exhausted and an electric valve (32) for opening and closing the carbon dioxide gas cylinder (2) for deflation. Each carbon dioxide gas cylinder (2) is provided with the sensor (31) and the electric valve (32). The central controller is electrically connected to the sensor (31) and the electric valve (32).

3. A carbon dioxide control system for a bone marrow cell culture box as claimed in claim 2, characterized in that: The replacement device also includes an alarm for reminding that all carbon dioxide gas cylinders (2) are exhausted, and the alarm is electrically connected to the central controller and the sensor (31).

4. A carbon dioxide control system for a bone marrow cell culture box as claimed in claim 3, characterized in that: The carbon dioxide gas cylinder (2) is also provided with a safety temperature detection component, which is electrically connected to the alarm component via the central controller.

5. A carbon dioxide control system for a bone marrow cell culture box as claimed in claim 1, characterized in that: The control system further comprises a buffer box (4) for pre-storing carbon dioxide, the buffer box (4) being located between the incubator (1) and the plurality of carbon dioxide gas cylinders (2), and a temperature control unit being arranged in the buffer box (4) for adjusting the temperature of the carbon dioxide before it is released into the incubator (1).

6. A carbon dioxide control system for a bone marrow cell culture box as claimed in claim 5, characterized in that: The temperature control unit comprises a temperature sensor (41) located in the incubator (1) and a temperature regulating element (42) located in the buffer box (4); the temperature sensor (41) and the temperature regulating element (42) are both electrically connected to the central controller.

7. A carbon dioxide control system for a bone marrow cell culture box as claimed in claim 6, characterized in that: The temperature regulating member (42) is an electric heating plate, and the electric heating plate is fixedly connected to the inner wall of the buffer box (4).

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