A new type of SPE column for Sudan red detection

Abstract

The utility model relates to the technical field of Sudan red detection in food, and discloses a new type of SPE column for Sudan red detection, including a detection column, a feed plate, a feed port, a sieve plate, a filler and a discharge port. In the utility model, the new type of Sudan red detection special SPE column adopts a modified polymer prepared with a polystyrene-divinylbenzene polymer as a matrix as a filler. The modified polystyrene-divinylbenzene polymer has a high degree of particle size uniformity, low nonspecific adsorption, and strong specific binding force. Compared with the national standard method, a more stable recovery rate can be obtained, the solvent usage is less and more environmentally friendly, and the method is also simpler. The experimental results show that the average spiked recovery rate of Sudan red in four different experimental samples of chili powder, chili oil, ham sausage and hot pot base is 87% to 102%.

Description

Technical Field

[0001] The utility model relates to the technical field of Sudan red detection in food, in particular to a novel SPE column dedicated for Sudan red detection. Background Art

[0002] As we all know, Sudan Red is a synthetic organic dye, commonly used as a colorant in food, medicine and cosmetics. It belongs to the category of azo dyes and has bright red or orange-red color. Due to its bright color and high stability, it is widely used in condiments, meat products, seafood, candy, beverages, mints, chewing gum and other foods to increase the attractiveness of the product. However, Sudan Red is also considered a harmful substance. Long-term intake of Sudan Red may pose a potential risk to human health. Therefore, all countries have formulated strict regulations and standards to restrict or prohibit the use of Sudan Red in food. Food safety agencies and regulatory authorities usually conduct Sudan Red testing to ensure that the Sudan Red content in food meets regulatory requirements and ensure the food safety of the public. Usually, Sudan Red testing uses a special SPE column as a pre-treatment method for separating and purifying Sudan Red dyes in food samples. It can effectively separate Sudan Red dyes and facilitate subsequent quantitative analysis by detectors.

[0003] However, a traditional new type of SPE column specially used for Sudan red detection uses a neutral alumina solid phase extraction column to purify the sample. The sample processing process of this method is complicated, the activity of alumina is difficult to control, and the recovery rate is difficult to guarantee. Therefore, technical improvements are urgently needed. Utility Model Content

[0004] The purpose of the utility model is to solve the shortcomings of the prior art, and a new type of SPE column for Sudan red detection is proposed. The new type of SPE column for Sudan red detection adopts a modified polymer prepared by using a polystyrene-divinylbenzene polymer as a matrix as a filler. The modified polystyrene-divinylbenzene polymer has a high degree of particle size uniformity, low nonspecific adsorption, and strong specific binding force. Compared with the national standard method, a more stable recovery rate can be obtained, the solvent usage is less and more environmentally friendly, and the method is simpler. The experimental results show that the average spiked recovery rate of Sudan red in four different experimental samples of chili powder, chili oil, ham sausage and hot pot base is 87% to 102%.

[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0006] A novel SPE column specially used for Sudan red detection comprises a detection column, wherein the upper end of the outer wall of the detection column is fixedly connected with a feed plate, a feed port is provided in the middle of the upper surface of the feed plate, and the feed port penetrates the feed plate to the interior of the detection column.

[0007] Through the above technical scheme, compared with the existing SPE column dedicated to Sudan red detection, when the new SPE column dedicated to Sudan red detection is used, a modified polymer prepared with polystyrene-divinylbenzene polymer as a matrix is ​​used as a filler, and its surface electrons can form pi-pi interactions with aromatic rings, and it has strong polar retention ability and Lewis acid characteristics, and is also very stable in a high pH environment, which is suitable for the extraction of aromatic amine compounds, and achieves a very strong target compound binding and separation effect.

[0008] Furthermore, a filler is provided at the lower end of the inner wall of the detection column, and a sieve plate is provided at the upper and lower ends of the outer wall of the filler, and the sieve plate at the lower end is tightly fitted with the bottom of the detection column;

[0009] Through the above technical solution, the filler can react physically or chemically with the chemical components in the sample, thereby achieving binding, separation and purification.

[0010] Furthermore, a discharge port is provided in the middle of the lower surface of the detection column;

[0011] Through the above technical solution, the sample can be separated in the column and the solution can flow out through the discharge port.

[0012] Furthermore, the filler is a modified polymer prepared with polystyrene-divinylbenzene polymer as a matrix;

[0013] Through the above technical solution, a very strong target compound binding and separation effect is achieved.

[0014] Furthermore, a blocking cover is provided at the lower end of the outer wall of the detection column;

[0015] Through the above technical solution, the lower end can be easily sealed by the plugging cover.

[0016] The utility model has the following beneficial effects:

[0017] 1. The utility model proposes a new type of SPE column for Sudan red detection. Compared with the existing Sudan red detection SPE column, the new type of Sudan red detection SPE column adopts a modified polymer prepared with polystyrene-divinylbenzene polymer as a matrix as a filler. Compared with the national standard method, a more stable recovery rate can be obtained, less solvent is used, more environmentally friendly, and the method is simpler. The experimental results show that the average spiked recovery rate of Sudan red in four different experimental samples, namely chili powder, chili oil, ham sausage and hot pot base, is 87% to 102%.

[0018] 2. The utility model proposes a new type of SPE column for Sudan red detection. Compared with the existing SPE column for Sudan red detection, the new type of SPE column for Sudan red detection adopts a modified polymer prepared with polystyrene-divinylbenzene polymer as a matrix as a filler. The surface electrons of the polymer can form pi-pi interactions with the aromatic rings, and the polymer has strong polar retention ability and Lewis acid properties. It is also very stable in a high pH environment, and is suitable for the extraction of aromatic amine compounds, thereby achieving a very strong target compound binding and separation effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is an axonometric diagram of a new type of SPE column specially used for Sudan red detection proposed in the utility model;

[0020] Figure 2 This is an explosion diagram of a new type of SPE column specially used for Sudan red detection proposed in the utility model;

[0021] Figure 3 This is an axial cross-sectional view of a new type of SPE column specially used for Sudan red detection proposed in the utility model;

[0022] Figure 4 for Figure 3 Enlarged view of point A in the middle.

[0023] 1. Detection column; 2. Feed plate; 3. Feed port; 4. Discharge port; 5. Plug cover; 6. Filler; 7. Sieve plate. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0025] Reference Figure 1-4 The utility model provides an embodiment: a new type of SPE column dedicated to Sudan red detection, including a detection column 1, the upper end of the outer wall of the detection column 1 is fixedly connected with a feed plate 2, and a feed port 3 is opened in the middle of the upper surface of the feed plate 2, and the feed port 3 passes through the feed plate 2 to the interior of the detection column 1.

[0026] Compared with the existing SPE column for Sudan red detection, this new SPE column for Sudan red detection adopts a modified polymer prepared with polystyrene-divinylbenzene polymer as the matrix as the filler. The surface electrons of the polymer can form pi-pi interactions with the aromatic rings, and the polymer has strong polar retention ability and Lewis acid properties. It is also very stable in a high pH environment and is suitable for the extraction of aromatic amine compounds, achieving very strong target compound binding and separation effects.

[0027] Compared with the traditional method of using neutral alumina, a new SPE column specially used for Sudan red detection avoids the use of toxic experimental solvents such as dichloromethane and n-hexane, which is more environmentally friendly and simpler.

[0028] A filler 6 is provided at the lower end of the inner wall of the detection column 1, and a sieve plate 7 is provided at the upper and lower ends of the outer wall of the filler 6. The lower sieve plate 7 fits tightly with the bottom of the detection column 1; the filler 6 can react physically or chemically with the chemical components in the sample, thereby achieving adsorption, separation and purification. A discharge port 4 is provided in the middle of the lower surface of the detection column 1; the discharge port facilitates the outflow of the solution after the sample is separated in the column. The filler 6 uses a modified polymer prepared with a polystyrene-divinylbenzene polymer as a matrix as a filler, which is more suitable for the extraction of aromatic amine compounds and achieves a very strong target compound binding and separation effect. A plugging cover 5 is provided at the lower end of the outer wall of the detection column 1; the plugging cover 5 facilitates the sealing of the lower end.

[0029] An application example of the utility model: when the new SPE column specially used for Sudan red detection is used, it is first activated with a methanol solution; the liquid to be purified in the extraction step is added to the new SPE column specially used for Sudan red detection for extraction; after the sample liquid is completely flowed out, it is rinsed with laboratory pure water; then it is eluted with a methanol solution, and the eluate is collected; after filtering with a 0.45μmPTFE organic filter membrane, HPLC analysis is performed.

[0030] Working principle: When in use, the active groups in the filler are activated, and then the sample to be tested is placed in the test column through the feed port. The sample to be tested enters the column from the feed port through negative pressure, passes through the modified polymer prepared with polystyrene-divinylbenzene polymer as the matrix as the filler, and the filler and the target compound can be combined in the SPE column, and other liquids flow out from the discharge port. After steps such as elution, the filler and the target compound are separated to obtain the target compound for subsequent HPLC analysis.

[0031] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A novel SPE column for Sudan red detection, comprising a detection column (1), characterized in that: A feed plate (2) is fixedly connected to the upper end of the outer wall of the detection column (1), a feed port (3) is provided in the middle of the upper surface of the feed plate (2), and the feed port (3) passes through the feed plate (2) to the interior of the detection column (1).

2. A novel SPE column for Sudan red detection according to claim 1, characterized in that: A filler (6) is provided at the lower end of the inner wall of the detection column (1), and a sieve plate (7) is provided at the upper and lower ends of the outer wall of the filler (6), and the sieve plate (7) at the lower end is tightly fitted to the bottom of the detection column (1).

3. A novel SPE column for Sudan red detection according to claim 1, characterized in that: A discharge port (4) is provided in the middle of the lower surface of the detection column (1).

4. A novel SPE column for Sudan red detection according to claim 2, characterized in that: The filler (6) is a modified polymer prepared with polystyrene-divinylbenzene polymer as a matrix.

5. A novel SPE column for Sudan red detection according to claim 1, characterized in that: The lower end of the outer wall of the detection column (1) is sleeved with a blocking cover (5).

Images