New ELISA Device

Abstract

A novel ELISA instrument device includes a power module, an electric linear slide, and an ELISA instrument body, characterized in that it also has a prompt circuit; the ELISA instrument body has two detectors, namely a CCD detector and a photomultiplier tube detector, and the electric linear slide is installed at the detector installation station at the lower end of the shell of the ELISA instrument body; a support plate is installed on the sliding block of the electric linear slide, and the lower ends of the CCD detector and the photomultiplier tube detector are respectively installed on the left and right sides of the upper end of the support plate; two-way prompt circuits and a power module are installed in the component compartment of the ELISA instrument body and are electrically connected. The novel device can conveniently switch between the CCD detector and the photomultiplier tube detector to detect the corresponding samples, and after the detector is in place, it can promptly prompt the detection personnel to enter the detection process through the light-emitting diode. The novel device brings convenience to the staff, saves the capital investment of the relevant departments in equipment, and can achieve better detection effects on the samples.

Description

Technical Field

[0001] This utility model relates to the field of detection equipment technology, and in particular to a novel enzyme-linked immunosorbent assay (ELISA) reader device. Background Technology

[0002] An ELISA reader is a crucial medical device with wide applications in hospitals, universities, research institutions, disease control centers, quality supervision bureaus, animal and plant quarantine, and the food and feed industries. In operation, the technician opens the lower front door of the ELISA reader, places the sample (located within the plastic microplate) on the internal tray, closes the door, and then controls the device to perform the test using buttons on the upper front of the ELISA reader. The core component of existing ELISA readers is the photodetector, a single detector, either based on a CCD (photodiode array) or a photomultiplier tube (PMT). During testing, light emitted from the light source at the upper part of the ELISA reader is filtered or monochromated into a single beam of light, which enters the sample within the plastic microplate. Part of this monochromatic light is absorbed by the sample, while the remaining portion passes through the transparent tray and illuminates the photodetector at the lower part of the ELISA reader. The photodetector inputs this light signal, varying in intensity depending on the sample, to the signal input terminal of the ELISA reader's conversion plate, which then converts it into a corresponding electrical signal. The electrical signal, after pre-amplification, logarithmic amplification, analog-to-digital conversion, and other signal processing, is sent to a microprocessor (with a detector power output terminal to control the detector's operation) for data processing and calculation. Finally, the data results of the tested sample (such as allergens, thyroid hormones, inorganic toxins, etc.) are displayed on the screen, or displayed through an external monitor and printer. {For the above background technology, please refer to the website link https: / / baike.baidu.com / item / %E9%85%B6%E6%A0%87%E4%BB%AA / 7312640?fr=ge_ala, the webpage states that "an ELISA reader is actually a phase-shift photoelectric colorimeter or spectrophotometer, and its basic working principle and main structure are basically the same as a photoelectric colorimeter. The light wave emitted by the light source is converted into a beam of monochromatic light through a filter or monochromator and enters the sample to be tested in the plastic microplate."}Part of the monochromatic light is absorbed by the specimen, while the other part passes through the specimen and illuminates the photodetector. The photodetector converts the light signals of varying intensity from the specimen into corresponding electrical signals. These electrical signals undergo pre-amplification, logarithmic amplification, and analog-to-digital conversion (this clearly indicates that although not explicitly stated, the photodetector actually has a conversion board that converts the light signals of varying intensity from the specimen into corresponding electrical signals, which are then pre-amplified, logarithmically amplified, and converted to digital before being output to the microprocessor). The microprocessor then processes and calculates the data, and finally, the results are displayed on a monitor and printed. Please also refer to the three patents: "2023220894181, 2023218583447, and 2023217657532". The patent describes that "during the detection process, the light emitted by the light source at the upper end of the detection chamber is filtered by a light filter or monochromator and becomes a beam of monochromatic light, which enters the sample to be tested in the transparent test tube. Then, part of the monochromatic light is absorbed by the sample, and the other part passes through the sample and illuminates the photodetector at the lower end of the detection chamber. The photodetector inputs the light signals of different intensities from the sample to the signal input terminal of the ELISA reader's conversion plate. The conversion plate converts them into corresponding electrical signals. After signal processing such as pre-amplification, logarithmic amplification, and analog-to-digital conversion, the electrical signals are sent to the microprocessor for data processing and calculation. Finally, the data results of the test sample (such as allergen, thyroid, inorganic toxin data, etc.) are displayed on the screen, or the data results are displayed and printed through the external display and printer."

[0003] In practical applications, CCD detectors have the advantage of fast detection speed, but their detection range for weak light signals is limited (affecting the detection effect of weak light signals). Photomultiplier tube detectors are more sensitive to weak light signals, but their suppression effect on strong light is not as good as that of CCD detectors, and their detection speed is slower (affecting the detection effect of some strong light signals). Therefore, both have certain advantages and disadvantages. In order to achieve effective detection of different samples, testing departments generally equip themselves with two types of microplate readers, one with a CCD detector and the other with a photomultiplier tube detector. This will bring inconvenience to actual use and increase the corresponding investment in equipment (the wiring and usage of the conversion plates and microprocessors for CCD detectors and photomultiplier tube detectors are completely the same, only the type of photodetector of the microplate reader is changed). With the development of industrial technology, ELISA reader technology has also made some progress. For example, the authorized patent in my country, patent number "201420612994.8," entitled "Innovative ELISA Reader," states that "this utility model has complete functions and is easy to use. When medical personnel perform ELISA testing, the operation is simple, saving time and effort, and reducing the workload of medical personnel." As can be seen above, although the patent achieves the described technical effects to a certain extent, due to structural limitations, like other devices in the field, it still cannot simultaneously combine the advantages of CCD detectors and photomultiplier tube detectors, and still suffers from the problems existing in the prior art. In conclusion, it is particularly necessary to provide a dual-detection ELISA reader that combines the advantages of high detection speed of CCD detectors and sensitivity to weak signal detection of photomultiplier tube detectors. Utility Model Content

[0004] To overcome the limitations of existing ELISA readers, which cannot effectively combine the advantages of CCD detectors and photomultiplier tube detectors due to structural constraints, and thus suffer from the drawbacks described in the background, this invention provides an ELISA reader based on the main body and accessories such as CCD detectors and photomultiplier tube detectors. In application, with the joint efforts of relevant institutions, testing personnel can conveniently switch between CCD detectors and photomultiplier tube detectors via a power switch to test the corresponding samples. Moreover, once the detector is in place, it can promptly prompt the testing personnel to enter the testing process, thereby bringing convenience to the staff, saving relevant departments money on equipment investment, and achieving better sample testing results.

[0005] The technical solution adopted by this utility model to solve its technical problem is:

[0006] A novel ELISA reader device includes a power module, an electric linear slide, and an ELISA reader body. Its distinguishing feature is the inclusion of a prompting circuit. The ELISA reader body has two types of detectors: a CCD detector and a photomultiplier tube detector. The electric linear slide is installed at the lower detector mounting position within the housing of the ELISA reader body. A support plate is mounted on the sliding block of the electric linear slide, and the lower ends of the CCD detector and the photomultiplier tube detector are respectively mounted on the left and right sides of the upper part of the support plate. The prompting circuit has two paths, and the two prompting circuits and the power module are installed in the component compartment of the ELISA reader body. The power output terminal of the power module is electrically connected to the power input terminals of the two prompting circuits.

[0007] Furthermore, each prompting circuit includes an electrically connected light-emitting diode, a resistor, and a reed switch. One end of the reed switch is connected to one end of the resistor, and the other end of the resistor is connected to the positive terminal of the light-emitting diode.

[0008] Furthermore, a magnet is installed on the upper front end of the housing of the electric linear slide, and reed switches for the two prompting circuits are installed on both sides of the lower front end of the support plate.

[0009] Furthermore, the reed switches in the two-way prompting circuit are normally open contact reed switches.

[0010] The beneficial effects of this invention are as follows: Based on the microplate reader body and accessories such as the CCD detector and photomultiplier tube detector, this invention allows for convenient control of the electric linear slide via a power switch. The CCD detector and photomultiplier tube detector are positioned at the lower center of the transparent tray, allowing for switching between them to detect the corresponding sample. Once the detectors are in position, LEDs promptly indicate to the operator that the detection process has begun. This invention provides convenience for staff, saves on equipment investment for relevant departments, and achieves better sample detection results. Based on the above, this invention has promising application prospects. Attached Figure Description

[0011] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0012] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0013] Figure 2 This is a partially enlarged structural schematic diagram of this utility model.

[0014] Figure 3 This is the circuit diagram of this utility model. Detailed Implementation

[0015] Figure 1 ,2 As shown in Figure 3, the novel ELISA reader device includes a power module U1, an electric linear slide M (a finished electric screw-type slide with a working voltage of DC 12V and a power of 20W), an ELISA reader body 1, a power switch S, and a prompting circuit 2; the ELISA reader body has two types of detectors, namely a CCD detector U2 and a photomultiplier tube detector U3. The housing of the electric linear slide M is laterally distributed and installed in the middle of the detector mounting position at the lower end of the housing of the ELISA reader body 1 via screws and nuts (in this embodiment, the detector mounting position of the housing is relatively high to ensure a suitable distance between the detector and the tray in the middle of the ELISA reader housing, and the lateral length of the housing is relatively large to ensure that both detectors can move to the middle of the lower end of the tray of the ELISA reader); A support plate 3 is horizontally mounted on the sliding block of the electric linear slide M via a screw and nut. The lower ends of the CCD detector U2 and the photomultiplier tube detector U3 are respectively mounted on the left and right sides of the upper end of the support plate 3 via screws and nuts. The signal output terminals of the CCD detector U2 and the photomultiplier tube detector U3 are connected in parallel and then connected to the signal input terminal of the conversion plate U5 inside the ELISA reader via wires. The power input terminals of the CCD detector U2 and the photomultiplier tube detector U3 are connected in series via power switches S1 and S2 and then connected in parallel to the power output terminal of the microprocessor detector U4 of the ELISA reader via wires. The prompting circuit 2 has two paths. The power switches S (S1, S2), the two prompting circuits 2, and the power module U1 are installed in the component compartment of the ELISA reader body 1.

[0016] Figure 1 , 2As shown in Figure 3, each prompting circuit includes a light-emitting diode VL1 or VL2 and a resistor R1 or R2 (installed in the component compartment) connected via circuit board wiring, and a reed switch GH1 or GH2. One end of the reed switch GH1 or GH2 is connected to one end of the resistor R1 or R2, and the other end of the resistor R1 or R2 is connected to the positive terminal of the light-emitting diode VL1 or VL2. A rectangular permanent magnet CT is glued to the middle of the upper front end of the electric linear slide's housing, and the upper end of the magnet CT is spaced a certain distance from the sliding block of the electric linear slide M and the lower end of the support plate 3. The reed switches GH1 or GH2 of the two prompting circuits are glued to the middle of the left side and the middle of the right side of the lower front end of the support plate 3, respectively. The moving contact of the reed switch GH1 or GH2 is horizontally located at the upper end, and the stationary contact is horizontally located at the lower end. The reed switches GH1 or GH2 of the two-way prompting circuit are normally open reed switches with glass shells. When the left and right middle positions of the support plate 3 move to the upper end of the magnet CT, the magnetism of the magnet CT acts on the two reed switches GH1 or GH2 respectively (the magnet is located 2 mm below the reed switch, and the wires connected to the two reed switches have length margin), and the moving and stationary contacts inside the two reed switches GH1 or GH2 close respectively. The power output terminals 3 and 4 and 5 and 6 of the power switch S are connected to the positive and negative and negative and positive power input terminals of the electric linear slide M respectively via wires. The power input terminals 1 and 2 of the power module U1 are connected to the 220V power line inside the microplate reader via wires. The power output terminals 3 and 4 of the power module U1, the power input terminals 1 and 2 of the power switch S, and the other end of the reed switch GH1 and the negative terminal of LED VL1, the other end of the reed switch GH2 and the negative terminal of LED VL2 are connected via wires.

[0017] Figure 1 , 2As shown in Figure 3, this novel ELISA reader is based on the main body 1, and the other detection methods are completely consistent with existing ELISA readers. In the application of the ELISA reader main body 1, the inspector opens the lower front door 4 of the outer shell, then places the sample to be tested (located inside the plastic microplate) on the inner tray. After closing the door 4, the inspector controls the device to detect the sample by operating the button 6 on the upper front of the outer shell. During the detection, the light wave emitted by the light source lamp at the upper end of the ELISA reader main body 1 is converted into a beam of monochromatic light through a filter or monochromator, and enters the sample to be tested in the plastic microplate. Then, part of the monochromatic light is absorbed by the sample, and the other part passes through the sample and illuminates the photodetector at the lower end of the ELISA reader through the transparent tray. On the CCD detector U2 or photomultiplier tube detector U3, the photodetector inputs light signals of varying intensity from the sample to the signal input terminal of the microplate reader conversion plate U5. The conversion plate U5 (whose signal output terminal is connected to the signal input terminal of the microprocessor U4 via a data cable) converts these signals into corresponding electrical signals. After pre-amplification, logarithmic amplification, analog-to-digital conversion, and other signal processing, the electrical signals are sent to the microprocessor U4 (which has a detector power output terminal to control the detector's operation) for data processing and calculation. Finally, the sample data results are displayed on the display screen 7, or displayed through an external monitor and printer. In this new invention, after the AC 220V power supply enters the power input terminal of the power module U1, the power output terminal of the power module U1 outputs a stable DC 12V power supply, which enters the power input terminals of the power switch S and the two indicator circuits. During testing, depending on the light transmittance and light sensitivity of the sample, the operator can easily control the electric linear slide via power switch S to position the CCD detector U2 and photomultiplier tube detector U3 at the lower center of the tray, thus switching between the CCD and photomultiplier tube detectors for testing the corresponding sample. Specifically, when the operator needs the photomultiplier tube detector U3 to be positioned at the lower end of the tray (after turning on power switch S2, which connects the power input of photomultiplier tube detector U3 to the detector power output of the microprocessor U4 of the microplate reader, enabling it to operate), the operator moves the handle of power switch S to the right. Therefore, pins 1 and 2 and pins 5 and 6 of the power switch S are connected respectively. After the negative and positive power input terminals of the electric linear slide M are energized, its sliding block drives the support plate to move to the left. In this way, the CCD detector U2 and the photomultiplier tube detector U3 move to the left. When the photomultiplier tube detector U3 moves to the middle of the lower end of the tray, the reed switch GH2 is just located at the upper end of the magnet CT. The internal contacts of the reed switch GH2 are closed. In this way, the 12V power supply enters the negative power input terminal of the light-emitting diode VL2 through the current limiting and voltage reduction of the resistor R2. The light-emitting diode VL2 is energized and lights up to indicate that the photomultiplier tube detector U3 is adjusted to the correct position. Then, the detection process can be started (power switch S is turned off).When the operator needs the CCD detector U2 to be positioned at the bottom of the tray (first turn on power switch S1 and turn off S2, so that the power input terminal of CCD detector U2 is connected to the detector power output terminal of the microprocessor U4 of the microplate reader, and it is powered on), the operator moves the handle of power switch S to the left. Then, pins 1 and 2 and pins 3 and 4 of power switch S are connected respectively. After the positive and negative power input terminals of the electric linear slide M are powered on, its sliding block drives the support plate to move to the right. In this way, CCD detector U2 and photomultiplier tube detector U3 move to the right. When CCD detector U2 moves to the middle of the bottom of the tray, reed switch GH1 is just above the magnet CT. The internal contacts of reed switch GH1 are closed. In this way, 12V power is limited and reduced by resistor R1 and enters the negative power input terminal of LED VL1. LED VL1 is powered on and lights up to indicate that CCD detector U2 is adjusted to the correct position. Then the detection process can be started (turn off power switch S).

[0018] Figure 1 , 2 As shown in Figure 3, during operation, the testing personnel can conveniently control the electric linear slide to position the CCD detector and photomultiplier tube detector at the lower center of the tray via a power switch. This allows for switching between the CCD and photomultiplier tube detectors to test the corresponding samples. Furthermore, once the detectors are in position, LEDs will promptly indicate to the testing personnel that the testing process can begin. This invention provides convenience for staff, saves relevant departments on equipment investment, and achieves better sample testing results. Figure 3 In the middle, the resistance values ​​of resistors R1 and R2 are 1.8K; the power module U1 is a finished AC 220V to DC 12V switching power supply module; LEDs VL1 and VL2 are red and green LEDs respectively (the light-emitting surfaces are located outside the first and second openings at the upper right end of the front of the housing, and all power switch handles are located outside the third, fourth and fifth openings at the upper right end of the front of the housing respectively).

[0019] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A novel microplate reader device, comprising a power module, an electric linear slide, and a microplate reader body, characterized in that: It also has a prompt circuit; the ELISA instrument body has two detectors, namely a CCD detector and a photomultiplier tube detector, and the electric linear slide is installed at the detector installation station at the lower end of the shell of the ELISA instrument body; a support plate is installed on the sliding block of the electric linear slide, and the lower ends of the CCD detector and the photomultiplier tube detector are respectively installed on the left and right sides of the upper end of the support plate; the prompt circuit has two paths, and the two-path prompt circuit and a power module are installed in the component compartment of the ELISA instrument body, and the power output end of the power module is electrically connected to the power input end of the two-path prompt circuit.

2. The novel microplate reader device according to claim 1, characterized in that: Each prompt circuit includes an electrically connected light emitting diode, a resistor and a reed switch. One end of the reed switch is connected to one end of the resistor, and the other end of the resistor is connected to the positive electrode of the light emitting diode.

3. The novel microplate reader device according to claim 1, characterized in that: A magnet is installed at the front upper side end of the shell of the electric linear slide, and reed switches of two-way prompt circuits are installed at both sides of the front lower end of the support plate.

4. The novel microplate reader device according to claim 1, characterized in that: The reed switch of the two-way prompt circuit is a normally open contact reed switch.

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