A kit for detecting vomitoxin

Abstract

The utility model relates to the technical field of detection kits, and specifically provides a kit for detecting vomitoxin, comprising a box body and a box cover, wherein a detection test strip is arranged in the box body; a sample addition hole and an observation hole are opened on the box cover; a filter is arranged in the sample addition hole; a transparent cover is arranged at the upper end of the observation hole; a detachable detection color card is matched on the transparent cover, and a plurality of color strips with different degrees of color change are arranged on the detection color card, and a single color strip corresponds to a range value of the content of vomitoxin; the utility model has reasonable design and simple structure, and the design of the transparent cover can prevent foreign matter from falling into the observation hole while not affecting the observation effect of CT line display; the setting of the detection color card can detect the range value of the content of vomitoxin in the sample; the detection color card is matched with the detachable design of the transparent cover, so that the detection color card can be reused, saving the detection cost.

Description

Technical Field

[0001] The utility model relates to the technical field of detection kits, and in particular to a kit for detecting vomitoxin. Background Art

[0002] The main component of vomitoxin is DON, a trichothecenes compound, mainly from the Fusarium genus, including Fusarium graminearum and Fusarium luteum. The contamination of vomitoxin DON in grains is related to the strain that produces the toxin, temperature, humidity, ventilation, sunshine and other factors. The main pollutants include cereal crops such as wheat, barley, oats, and corn; grain products such as bread, biscuits, and wheat snacks; and animal milk and eggs. Wheat fusarium wilt is mainly distributed in humid temperate regions, and most parts of my country are in this zone, so the pollution of vomitoxin DON is serious, and the pollution situation is more serious in rainy years. This has caused great losses to feed companies and breeders. People eating food containing vomitoxin will endanger human health. Therefore, the quantitative detection of vomitoxin in samples is particularly important.

[0003] Different treatment methods for cereal crops vary depending on the content of vomitoxin. If the content of vomitoxin in cereal crops is low and only exists on the surface or is generally low, they can be treated by shelling, screening, and heat sterilization. Cereal crops with high vomitoxin content can only be discarded. The kits used in the prior art can only detect whether there is vomitoxin in the sample, but cannot specifically detect the specific content value range of the sample, which is inconvenient for the treatment of cereal crops infected with vomitoxin. Utility Model Content

[0004] In view of the shortcomings of the prior art described above, the purpose of the present invention is to provide a kit for detecting vomitoxin, which is used to solve the problem that the kit used in the prior art can only detect whether vomitoxin exists in a sample, but cannot specifically detect the specific content value range of the sample.

[0005] In order to achieve the above-mentioned purpose and other related purposes, the utility model provides a kit for detecting vomitoxin, comprising a box body, a box cover is matched at the upper end of the box body, and a detection test strip is arranged in the box body;

[0006] The box cover is provided with a sample adding hole and an observation hole;

[0007] A filter screen is provided in the sample addition hole;

[0008] A transparent cover is provided at the upper end of the observation hole, and the transparent cover is connected to the box cover;

[0009] The transparent cover is equipped with a detachable detection color card, on which a plurality of color strips with different color change depths are arranged, and a single color strip corresponds to a range value of the vomitoxin content;

[0010] The transparent cover is provided with convex rails on the opposite side ends, and the side ends of the detection color card are provided with slide grooves, which are slidably matched with the convex rails; the content range of vomitoxin is determined by comparing the display line of the test paper strip with the color bar on the detection color card.

[0011] In one embodiment of the utility model, the test strip comprises a substrate and a sample pad, a binding pad, a nitrocellulose membrane, and a water-absorbing pad bonded to the substrate and overlapped in sequence;

[0012] The nitrocellulose membrane is provided with a T line and a C line, wherein the T line is arranged close to the binding pad side, and the C line is arranged close to the water absorption pad side;

[0013] The sample loading hole is located above the sample pad, and the observation hole is located above the T-line and C-line areas of the nitrocellulose membrane.

[0014] In one embodiment of the present invention, the conjugate pad contains DON antibodies labeled with fluorescent microspheres, the T-line is embedded with DON antigen, and the C-line is embedded with goat anti-rabbit secondary antibody.

[0015] In one embodiment of the present invention, the width of the color bar on the detection color card is the same as the width of the T line on the nitrocellulose membrane.

[0016] In an embodiment of the present invention, the transparent cover is configured to be rectangular.

[0017] In an embodiment of the present invention, the detection color card is configured to be U-shaped, and a plurality of color strips are respectively distributed on two side ends of the detection color card.

[0018] As described above, the kit for detecting vomitoxin of the utility model has the following beneficial effects:

[0019] 1. The transparent cover shell used in the utility model can form a landing point on the upper end of the box cover corresponding to the display line of the test paper strip in the observation box body, so that the detection color card can slide and cooperate with the transparent cover shell to adaptably correspond to the display line of the test paper strip, thereby determining the range of the vomitoxin content in the sample, improving the accuracy and detection effect of the sample detection, and then determining the vomitoxin contamination of the sampled product, so as to facilitate the adoption of corresponding treatment measures;

[0020] 2. The transparent cover shell provided in the utility model can also prevent foreign matter from falling into the observation hole without affecting the observation effect of CT line display; the filter can filter the particulate matter in the sample solution to be tested, and prevent the sample solution with particulate matter from entering the test paper strip from the sample addition hole to cause blockage, unclear color development and other abnormal phenomena; the overall design is reasonable, the structure is simple, and it can be applied to the accurate detection of the vomitoxin content in the sample, and it is highly practical. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 Shown is a schematic diagram of the main structure of the utility model.

[0022] Figure 2 Shown is a side structural schematic diagram of the utility model.

[0023] Figure 3 Shown is a cross-sectional structural schematic diagram of the present utility model.

[0024] Component number description

[0025] Box body 1; box cover 2; sample addition hole 21; observation hole 22; substrate 3; sample pad 31, binding pad 32; nitrocellulose membrane 33; T line 331; C line 332; water absorption pad 34; filter screen 4; transparent cover 5; convex rail 51; detection color card 6; slide 61; color strip 62. DETAILED DESCRIPTION

[0026] The following is a description of the implementation of the present invention by means of specific embodiments. People familiar with the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.

[0027] See also Figures 1 to 3 It should be noted that the structures, proportions, sizes, etc. illustrated in the drawings in this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the limiting conditions for the implementation of the utility model, so they have no substantial technical significance. Any modification of the structure, change in the proportion relationship, or adjustment of the size should still fall within the scope of the technical content disclosed by the utility model without affecting the effects and purposes that can be achieved by the utility model. At the same time, the terms such as "upper", "lower", "left", "right", "middle" and "one" quoted in this specification are only for the convenience of description, and are not used to limit the scope of the implementation of the utility model. The change or adjustment of their relative relationship should also be regarded as the scope of the implementation of the utility model without substantially changing the technical content.

[0028] See also Figure 1The utility model provides a kit for detecting vomitoxin, comprising a box body 1, a box cover 2 is matched at the upper end of the box body 1, and a test strip is arranged in the box body 1, the test strip comprises a substrate 3 and a sample pad 31, a conjugation pad 32, a nitrocellulose membrane 33, and a water-absorbing pad 34 which are bonded to the substrate 3 and overlapped in sequence; a T line 331 and a C line 332 are arranged on the nitrocellulose membrane 33, the T line 331 is arranged close to the conjugation pad 32, and the C line 332 is arranged close to the water-absorbing pad 34; the conjugation pad 32 has a vomitoxin antibody labeled with fluorescent microspheres, the T line 331 is embedded with a vomitoxin antigen, and the C line 332 is embedded with a goat anti-rabbit secondary antibody. The box cover 2 is provided with a sample loading hole 21 and an observation hole 22, the sample loading hole 21 is located above the sample pad 31, and the observation hole 22 is located above the area where the T line 331 and the C line 332 are arranged on the nitrocellulose membrane 33. When used specifically, the sample is dripped onto the test paper strip through the sample addition hole 21, and the vomitoxin in the sample solution to be tested is combined with the vomitoxin antibody labeled with the fluorescent microspheres in the conjugate pad 32 and chromatographed forward by capillary action. After reaching the detection area, the vomitoxin antibody antigen fixed on the T line 331 is combined with the remaining unbound vomitoxin antibody labeled with the fluorescent microspheres; the concentration of the vomitoxin antibody labeled with the fluorescent microspheres bound on the T line 331 is inversely proportional to the concentration of the vomitoxin in the sample, and the concentration of the fluorescent marker bound to the C line 332 is independent of the concentration of the vomitoxin in the sample.

[0029] Since the main pollutants of vomitoxin are cereal crops such as wheat, barley, oats, and corn, particulate matter is inevitably present when the cereal crops are sampled; the utility model provides a filter screen 4 in the sample addition hole 21, and the filter screen 4 can filter the particulate matter in the sample solution to be tested, thereby preventing the sample solution to be tested with particulate matter from entering the test paper strip from the sample addition hole 21 to cause abnormal phenomena such as blockage and unclear color development.

[0030] See also Figure 1-3A transparent cover shell 5 is provided at the upper end of the observation hole 22, and the transparent cover shell 5 is set to be rectangular; the transparent cover shell 5 is connected to the box cover 2; a detachable detection color card 6 is matched on the transparent cover shell 5, and the detection color card 6 is set to be U-shaped; a plurality of color strips 62 with different color change depths are provided on the detection color card 6, and a single color strip 62 corresponds to a range value of the vomitoxin content; the plurality of color strips 62 are respectively distributed on both side ends of the U-shaped structure of the detection color card 6. The transparent cover 5 is provided with convex rails 51 on the opposite sides, and the side ends of the detection color card 6 are provided with a slide groove 61, which slides with the convex rail 51; the display line of the detection test strip is compared with the color strip 62 on the detection color card 6 to determine the range of the content of vomitoxin in the sample; the transparent cover 5 used in the utility model can form a landing point corresponding to the display line of the detection test strip in the observation box body 1 at the upper end of the box cover 2, so that the detection color card can slide and match the transparent cover 5 to adaptably correspond to the display line of the detection test strip, thereby determining the range of the content of vomitoxin in the sample, improving the accuracy and detection effect of the sample detection, and then determining the vomitoxin contamination of the sampled product, so as to facilitate the adoption of corresponding treatment measures. In addition, since the detection color card 6 is detachable, after a single test kit detects the color and compares it with the detection color card 6, the detection color card 6 can be removed and reused, saving detection costs.

[0031] The width of the color bar 62 on the detection color card 6 is the same as the width of the T line 331 on the nitrocellulose membrane 33 , so that the T line 331 can be compared with the color bar 62 .

[0032] In summary, the kit for detecting vomitoxin provided by the utility model has a reasonable design and a simple structure. The design of the transparent cover 5 can prevent foreign matter from falling into the observation hole while not affecting the observation effect of the CT line display sample; the setting of the detection color card 6 can detect the range of the content of vomitoxin in the sample; the detection color card 6 is designed to be detachable with the transparent cover 5, so that the detection color card 6 can be reused to save the detection cost. Therefore, the utility model effectively overcomes various shortcomings in the prior art and has a high industrial utilization value.

[0033] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the technology may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed in the present invention shall still be covered by the claims of the present invention.

Claims

1. A kit for detecting vomitoxin, comprising a box body (1), a box cover (2) being provided at the upper end of the box body (1), and a test strip being arranged in the box body (1); Features: The box cover (2) is provided with a sample addition hole (21) and an observation hole (22); A filter screen (4) is provided in the sample addition hole (21); A transparent cover (5) is provided at the upper end of the observation hole (22), and the transparent cover (5) is connected to the box cover (2); The transparent cover (5) is equipped with a detachable detection color card (6), and the detection color card (6) is provided with a plurality of color strips (62) with different degrees of color change, and a single color strip (62) corresponds to a range value of the vomitoxin content; The transparent cover (5) is provided with convex rails (51) on the opposite side ends, and the detection color card (6) is provided with a slide groove (61) on the side end, and the slide groove (61) and the convex rail (51) are slidably matched; the content range value of vomitoxin is determined by comparing the display line of the test paper strip with the color bar (62) on the detection color card (6).

2. The kit for detecting vomitoxin according to claim 1, characterized in that: The test paper strip comprises a substrate (3) and a sample pad (31), a binding pad (32), a nitrocellulose membrane (33), and a water-absorbing pad (34) which are bonded to the substrate (3) and overlapped in sequence; The nitrocellulose membrane (33) is provided with a T line (331) and a C line (332), wherein the T line (331) is arranged close to the binding pad (32), and the C line (332) is arranged close to the water absorption pad (34); The sample addition hole (21) is located above the sample pad (31), and the observation hole (22) is located above the area where the T line (331) and the C line (332) are set on the nitrocellulose membrane (33).

3. The kit for detecting vomitoxin according to claim 2, characterized in that: The conjugate pad (32) contains a deoxynivalenol antibody labeled with fluorescent microspheres, the T line (331) contains a deoxynivalenol antigen, and the C line (332) contains a goat anti-rabbit secondary antibody.

4. The kit for detecting vomitoxin according to claim 3, characterized in that: The width of the color strip (62) on the detection color card (6) is the same as the width of the T line (331) on the nitrocellulose membrane (33).

5. The kit for detecting vomitoxin according to claim 1, characterized in that: The transparent cover (5) is arranged in a rectangular shape.

6. The kit for detecting vomitoxin according to claim 5, characterized in that: The detection color card (6) is configured to be U-shaped, and a plurality of color strips (62) are respectively distributed on both side ends of the detection color card (6).

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