A protein immunoblotting device

Abstract

The utility model relates to the field of biochemical technology, and provides a protein immunoblotting device, including an incubation tray; a shaking mechanism, the incubation tray is installed on the top of the shaking mechanism; a processing platform, the shaking mechanism is fixed to the processing platform; a liquid adding mechanism, located at the top of the incubation tray, adding reagents and lotions to the incubation device; a liquid discharging mechanism, located at the bottom of the processing platform, connected to the incubation tray through a circulation pipeline; a control mechanism, the control mechanism is electrically connected to the shaking mechanism, the liquid adding mechanism and the liquid discharging mechanism; the shaking mechanism includes an incubation tray rack, a driving structure and a sliding structure. The utility model adds culture reagents to the incubation tray through the liquid adding mechanism arranged on the top of the incubation tray, and the driving mechanism and the sliding mechanism of the shaking mechanism drive the incubation tray rack to shake in the horizontal direction, so that the experimental membrane in the incubation tray is fully integrated with the culture reagent. At the same time, a liquid discharging mechanism is also provided, and the overall automation and intelligence are high, which reduces manual operations and improves experimental efficiency and accuracy.

Description

Technical Field

[0001] The utility model relates to the field of biochemical technology, in particular to a protein immunoblotting device. Background Art

[0002] Western Blot experiment is a protein immunoblotting experiment, which is a protein analysis technique that is often used to identify a certain protein and perform qualitative and semi-quantitative analysis of the protein. In protein immunoblotting experiments, the experimental steps involving incubation membrane blocking and incubation are extremely critical, and the experimental operation is generally performed manually with the assistance of semi-automatic equipment.

[0003] According to investigation, it is difficult to guarantee the accuracy of the results of WB experiments. Because the WB experiment has many steps and each step requires many factors to be controlled, manual operation can easily cause errors to expand, and it is difficult to ensure repeatability for the same repeated experiments. During the experiment, the same step needs to be repeated, which takes a long time. The experimenter does the same action for several hours, which can easily lead to fatigue and experimental errors. The manual operation process is complicated and imprecise. Repeated operations result in large manual errors, making the protein blotting process inefficient and ineffective. Utility Model Content

[0004] In order to solve the deficiencies in the above-mentioned prior art, the utility model provides a protein immunoblotting device, including an incubation tray; a shaking mechanism, the incubation tray is installed on the top of the shaking mechanism; a processing platform, the shaking mechanism is fixed to the processing platform; a liquid adding mechanism, located at the top of the incubation tray, adding reagents and lotions to the incubation device; a liquid discharge mechanism, located at the bottom of the processing platform, connected to the incubation tray through a circulation pipeline; a control mechanism, the control mechanism is electrically connected to the shaking mechanism, the liquid adding mechanism and the liquid discharge mechanism; the shaking mechanism includes an incubation tray rack, a driving structure and a sliding structure, the incubation tray is installed on the incubation tray rack, the driving structure and the sliding structure are installed at the bottom of the incubation tray rack, and the driving structure drives the incubation tray rack to slide on the upper part of the sliding structure.

[0005] Furthermore, the incubation tray includes a solution portion, an extension portion and a retaining member, wherein the extension portion is arranged on the top side of the solution portion and is perpendicular to the side of the solution portion; the retaining member is arranged on the outside of the extension portion and is clamped under the incubation tray rack.

[0006] Furthermore, the driving structure includes a motor, a synchronous wheel, a synchronous belt, a transmission shaft, a cam and a cam follower. The motor drives the synchronous wheel and the synchronous belt to drive the synchronous belt and the synchronous wheel. The transmission shaft passes through the synchronous wheel and the cam, and the transmission shaft is eccentrically installed on the cam to drive the cam to rotate.

[0007] Furthermore, the cam follower includes a first fixed seat and a second fixed seat, the first fixed seat and the second fixed seat are installed at the bottom of the incubation tray rack, the first fixed seat and the second fixed seat are relatively provided with limit blocks, the limit blocks are arranged perpendicular to the fixed seats, and the side of the cam is against the limit blocks; the installation point of the transmission shaft on the cam coincides with the center of the line between the limit blocks of the first fixed seat and the second fixed seat, and the center of the cam does not coincide with the center of the line between the limit blocks.

[0008] Furthermore, the sliding structure includes a slide rail and a slider, the slider is installed at the bottom of the incubation tray rack, the slide rail is fixed to the top of the processing platform, and the slider slides back and forth linearly relative to the slide rail.

[0009] Furthermore, the liquid adding mechanism includes an antibody tube, a detergent sink, an electromagnetic valve tube clamp and a liquid adding peristaltic pump. The electromagnetic valve tube clamp is arranged on the flow pipe at the bottom of the antibody tube, and the liquid adding peristaltic pump is installed on the detergent sink.

[0010] Furthermore, the drainage mechanism includes a drainage peristaltic pump, an electromagnetic valve tube clamp and a waste liquid pool. An opening is provided at the bottom of the incubation plate, which is connected to the electromagnetic valve tube clamp and the drainage peristaltic pump through a circulation pipeline to discharge the waste liquid into the waste liquid pool.

[0011] Furthermore, the protein immunoblotting device is also provided with a chassis, the processing platform is arranged on the top of the chassis, and the drainage mechanism and the driving structure are arranged inside the chassis.

[0012] Furthermore, it also includes a heat dissipation mechanism, which is arranged inside the chassis and opposite to the driving structure; the heat dissipation mechanism includes an installation bin and a fan, the installation bin is fixedly installed on the chassis base, the fan is installed in the inner cavity of the installation bin, and the surface of the installation bin is provided with through holes for air circulation.

[0013] Furthermore, the control mechanism includes a controller and a display screen, and the controller is electrically connected to the display screen, the driving structure, the liquid adding mechanism, and the liquid discharging mechanism.

[0014] Based on the above, compared with the prior art, the utility model provides a protein immunoblotting device, which adds culture reagent to the incubation tray through a liquid adding mechanism arranged on the top of the incubation tray, and the driving mechanism and sliding mechanism of the shaking mechanism drive the incubation tray rack to shake in the horizontal direction, so that the experimental membrane in the incubation tray is fully integrated with the culture reagent. At the same time, a liquid discharge mechanism is also provided to facilitate the timely removal of the culture reagent. The overall automation and intelligence are high, manual operation is reduced, experimental efficiency and accuracy are improved, and it has good application value.

[0015] Other features and beneficial effects of the utility model will be described in the subsequent description, and partly become apparent from the description, or understood by practicing the utility model. The purpose and other beneficial effects of the utility model can be achieved and obtained by the structures specifically pointed out in the description, claims and drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the following is a brief introduction to the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work. The positional relationships described in the drawings in the following description are based on the directions of the components shown in the diagrams unless otherwise specified.

[0017] Figure 1 A schematic diagram of the three-dimensional structure of the protein immunoblotting device provided by the utility model;

[0018] Figure 2 This is a schematic diagram of the front structure of the protein immunoblotting device provided by the utility model;

[0019] Figure 3 A schematic diagram of the structure of the incubation tray provided by the utility model;

[0020] Figure 4 This is a front structural schematic diagram of the shaking mechanism provided by the utility model;

[0021] Figure 5 This is a schematic diagram of the bottom structure of the shaking mechanism provided by the utility model;

[0022] Figure 6 This is a front structural schematic diagram of the protein immunoblotting device provided by the utility model with part of the chassis and the drainage mechanism removed;

[0023] Figure 7 A schematic diagram of the three-dimensional structure of the protein immunoblotting device provided by the utility model with part of the chassis and the drainage mechanism removed;

[0024] Figure 8 This is a structural schematic diagram of the waterproof guide plate provided by the utility model.

[0025] Reference numerals:

[0026] DETAILED DESCRIPTION

[0027] In order to make the purpose, technical solutions and advantages of the embodiments of the utility model clearer, the technical solutions in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, rather than all of the embodiments; the technical features designed in different implementation modes of the utility model described below can be combined with each other as long as they do not conflict with each other; based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.

[0028] In the description of the present invention, it should be noted that all terms (including technical terms and scientific terms) used in the present invention have the same meanings as those generally understood by ordinary technicians in the field to which the present invention belongs, and cannot be understood as limitations on the present invention; it should be further understood that the terms used in the present invention should be understood to have meanings consistent with the meanings of these terms in the context of this specification and in the relevant fields, and should not be understood in an idealized or overly formal sense, unless explicitly defined in this utility model.

[0029] Currently, protein blotting in the industry is done manually. The steps of manual operation are as follows:

[0030] 1. Pour the blocking solution into the incubation tray 100, and then put the experimental membrane into it for immersion and blocking.

[0031] 2. After the sealing is completed, take out the experimental membrane with nylon tweezers, put it into the incubation tray 100 filled with detergent, shake it left and right to rinse, and rinse it three times.

[0032] 3. Take out the rinsed experimental membrane and put it into an empty incubation tray 100, then add the primary antibody reagent into the incubation tray 100, and shake the incubation tray 100 for incubation.

[0033] 4. After the incubation is completed, take out the experimental membrane with nylon tweezers, put it into the incubation tray 100 filled with detergent, and rinse it by shaking it left and right. Rinse it three times.

[0034] 5. Take out the rinsed experimental membrane and put it into an empty incubation tray 100, then add the secondary antibody reagent into the incubation tray 100, and shake the incubation tray 100 for incubation.

[0035] 6. After the incubation is completed, take out the experimental membrane with nylon tweezers, put it into the incubation tray 100 filled with detergent, and rinse it by shaking it left and right. Rinse it three times.

[0036] 7. Remove the experimental film.

[0037] The above is a complete manual experiment process. The above is a manual experiment process. It can be seen that the manual operation process is complicated, the manual operation time is not accurate, and the manual error is large due to repeated operations, which makes the protein blotting process inefficient.

[0038] In order to solve the above technical problems, the present invention provides a protein immunoblotting device, such as Figure 1 and Figure 2 As shown, it includes a liquid adding mechanism 300, an incubation tray 100, a shaking mechanism 200, a liquid discharging mechanism 400, a control mechanism 500, a heat dissipation mechanism 600, and a support frame 1, a processing platform 2 and a chassis 3 constituting the overall framework of protein immunoblotting. The incubation tray 100 is installed on the top of the shaking mechanism 200, and the shaking mechanism 200 is fixed to the processing platform 2.

[0039] In one embodiment, if Figure 3 As shown, the incubation tray 100 includes a solution portion 110, an extension portion 120 and a clamping member 130. The extension portion 120 is arranged on the top side of the solution portion 110 and is arranged perpendicular to the side of the solution portion 110. The clamping member 130 is arranged on the outer wall of the extension portion 120 and is clamped under the incubation tray frame 210. The top of the incubation tray 100 is also provided with an incubation tray 100 cover, and the incubation tray 100 cover is also provided with a perforation for a flow pipe 350 for adding liquid to pass through.

[0040] Preferably, the bottom surface of the solution portion 110 is composed of a first slope and a second slope, the angle between the first slope and the horizontal line is α, and the angle between the second slope and the horizontal plane is β. Preferably, the angle β is greater than the angle α. The design of the bottom slope can make the solution shake more fully during the shaking process, thereby ensuring the accuracy of the experimental results.

[0041] In one embodiment, the liquid adding mechanism 300 is located on the top of the incubation tray 100 to add reagents and detergents to the incubation device; the liquid adding mechanism 300 includes an antibody tube 310, a detergent sink 320, a solenoid valve tube clamp 330 and a liquid adding peristaltic pump 340, the solenoid valve tube clamp 330 is arranged on the pipeline at the bottom of the antibody tube 310, and the liquid adding peristaltic pump 340 is installed on the detergent sink 320; the antibody tube 310 includes a first antibody tube and a second antibody tube.

[0042] In specific implementation, the solenoid valve clamp 330 controls the addition of liquid to the first antibody tube, the second antibody tube and the detergent tank 320 respectively. There are primary antibody reagents and secondary antibody reagents in the first antibody tube and the second antibody tube. After the solenoid valve clamp 330 corresponding to each antibody tube 310 is opened, the corresponding primary antibody reagent or secondary antibody reagent is added to the corresponding incubation tray 100. When the addition is completed, the liquid addition peristaltic pump 340 extracts the detergent in the detergent tank 320 and adds it to the incubation tray 100 through the circulation pipe 350 to clean the experimental membrane and the tube wall of the antibody tube 310.

[0043] In one embodiment, if Figure 2 and Figure 4 As shown, the shaking mechanism 200 includes an incubation tray frame 210, a driving structure 220 and a sliding structure 230. The incubation tray 100 is installed on the incubation tray frame 210. The driving structure 220 and the sliding structure 230 are installed at the bottom of the incubation tray frame 210. The driving structure 220 drives the incubation tray frame 210 to slide on the upper part of the sliding structure 230. The shaking mechanism 200 is fixed to the processing platform 2; the bottom of the processing platform 2 is also provided with reinforcing ribs to make the entire processing process more stable.

[0044] Specifically, the incubation tray rack 210 is provided with a plurality of through mounting portions, through which the incubation tray 100 is fixedly installed, the bottom end of the extension portion 120 of the incubation tray 100 is abutted against the top of the incubation tray rack 210, and the locking piece 130 of the incubation tray 100 is locked at the bottom of the incubation tray rack 210, and the extension portion 120 and the locking piece 130 of the incubation tray 100 cooperate to firmly lock the incubation tray 100 on the incubation tray rack 210.

[0045] In one embodiment, if Figure 5 As shown, the driving structure 220 includes a motor 221, a synchronous wheel 222, a synchronous belt 223, a transmission shaft 224, a cam 225 and a cam follower 226. The motor 221 drives the synchronous wheel 222 and the synchronous belt 223 to drive the synchronous belt 223 and the synchronous wheel 222. The transmission shaft 224 passes through the synchronous wheel 222 and the cam 225, and the transmission shaft 224 is eccentrically installed on the cam 225 to drive the cam 225 to rotate.

[0046] The cam follower 226 includes a first fixed seat 2261 and a second fixed seat 2262, and the first fixed seat 2261 and the second fixed seat 2262 are installed at the bottom of the incubation tray rack 210. The first fixed seat 2261 and the second fixed seat 2262 are relatively provided with a limit block 2263, and the limit block 2263 is arranged perpendicular to the fixed seat, and the side of the cam 225 is against the limit block 2263; the transmission shaft 224 coincides with the center of the line connecting the first fixed seat 2261 and the second fixed seat 2262 at the installation point of the cam 225, and the center of the cam 225 does not coincide with the center of the line connecting the limit block 2263.

[0047] Preferably, the installation point of the transmission shaft 224 at the cam 225 coincides with the center of the line connecting the first fixed seat 2261 and the second fixed seat 2262 and the limit block 2263, while the center of the cam 225 does not coincide with the center of the line connecting the limit block 2263, which makes it possible that when the transmission shaft 224 drives the cam 225 to rotate, the line distance between the installation point of the transmission shaft 224 and the side of the cam 225 and the abutment point of the limit block 2263 has two states, from large to small and then from small to large. The cam 225 adjusts the distance by rotating, thereby pushing the distance between the two limit blocks 2263, and then adjusting the distance between the first fixed seat 2261 and the second fixed seat 2262, thereby driving the incubation tray rack 210 to slide back and forth on the sliding structure 230.

[0048] In one embodiment, the sliding structure 230 includes a slide rail 231 and a slider 232 . The slider 232 is installed at the bottom of the incubation tray rack 210 . The slide rail 231 is fixed to the top of the processing platform 2 . The slider 232 slides linearly back and forth relative to the slide rail 231 .

[0049] During specific implementation, the motion torque is transmitted to the synchronous wheel 222 through the motor 221, and then transmitted to the transmission shaft 224 through the synchronous belt 223, and then transmitted to the cam 225. The cam 225 drives the cam follower 226 to reciprocate, pushing the incubation tray rack 210 to move on the slide rail 231, thereby driving the incubation tray 100 to follow the incubation tray rack 210 to perform a horizontal shaking motion, allowing the antibody reagent to fully contact and react with the experimental membrane in the incubation tray 100.

[0050] In one embodiment, the drainage mechanism 400 is located at the bottom of the processing platform 2 and is connected to the incubation tray 100 through a circulation pipe 350; the drainage mechanism 400 includes a drainage peristaltic pump 410, an electromagnetic valve tube clamp 330 and a waste liquid pool. An opening is provided at the bottom of the incubation tray 100, which is connected to the electromagnetic valve tube clamp 330 and the drainage peristaltic pump 410 through the circulation pipe 350 to discharge the waste liquid into the waste liquid pool.

[0051] During specific implementation, the incubation tray 100 is connected to the electromagnetic valve pipe clamp 330 through the circulation pipe 350, and the electromagnetic valve pipe clamp 330 is connected to the drainage peristaltic pump 410 through the circulation pipe 350. After the reaction in the incubation tray 100 is completed, the waste liquid in the test tray is extracted by the drainage peristaltic pump 410, and the electromagnetic valve pipe clamp 330 controls the on-off of the corresponding circulation pipe 350 of each incubation tray 100, and then the waste liquid is discharged into the external waste liquid pool after being collected left and right. Preferably, because the incubation tray 100 needs to be driven by the shaking mechanism 200 to shake left and right, the circulation pipe 350 of the drainage mechanism 400 uses a hose, which can ensure the sealing of the circulation pipe 350 when the incubation tray 100 is shaken.

[0052] In one embodiment, if Figure 6As shown, the protein immunoblotting device also includes a heat dissipation mechanism 600, which is arranged inside the chassis 3 and opposite to the driving structure 220; the heat dissipation mechanism 600 includes a mounting chamber 610 and a fan 620, the mounting chamber 610 is fixedly installed on the base of the chassis 3, the fan 620 is installed in the inner cavity of the mounting chamber 610, and the surface of the mounting chamber 610 is provided with through holes 630 for air circulation.

[0053] In one embodiment, the control mechanism 500 is electrically connected to the shaking mechanism 200, the liquid adding mechanism 300 and the liquid discharging mechanism 400; Figure 1 and Figure 6 As shown, the control mechanism 500 includes a controller 510 and a display screen 520 , and the controller 510 is electrically connected to the display screen 520 , the driving structure 220 , the liquid adding mechanism 300 , and the liquid discharging mechanism 400 .

[0054] In specific implementation, the display screen 520 is a touch screen, which can be used for operation settings. The display screen 520 transmits the operation information to the controller 510. The controller 510 is electrically connected to the driving structure 220, the liquid adding mechanism 300, the liquid discharging mechanism 400 and the heat dissipation mechanism 600, and controls the liquid adding, liquid discharging and heat dissipation by controlling the on-off of the circuit. Preferably, the controller 510 uses PLC to ensure the operation of the entire device.

[0055] Preferably, the protein immunoblotting device as a whole is composed of a bottom chassis 3, a processing platform 2 on the top of the chassis 3, and a support frame 1 on the top of the processing platform 2 to form an external overall frame. The bottom chassis 3 is hollow inside, the driving structure 220, the drainage mechanism 400 and the heat dissipation structure are built inside the chassis 3, and the display screen 520 of the control mechanism 500 is installed outside the chassis 3, and the width direction cross section of the chassis 3 is a trapezoid, and the two side walls are set at a certain angle to the horizontal plane, and the display screen 520 is installed on the inclined side wall for easy viewing and operation.

[0056] In one embodiment, if Figure 7 As shown, a DC step-down module 700 is also provided inside the chassis 3 to reduce the high-voltage DC power to low DC power for the equipment to operate. A waterproof guide plate 800 is provided on the top of the DC step-down module 700. Figure 8 As shown, the waterproof guide plate 800 includes a guide portion 810, a connection portion 820, a flow-through portion 830 and a support rod 840. The two ends of the connection portion 820 are vertically connected to the guide portion 810 and the flow-through portion 830. Water drips onto the guide portion 810, flows along the guide portion 810 through the connection portion 820 to the flow-through portion 830 and flows out. Support rods 840 are provided at both ends of the guide portion 810 and the flow-through portion 820, and the connection between the guide portion 810 and the flow-through portion 830 and the connection portion 820 is rounded to facilitate the flow of water. The waterproof guide plate 800 prevents liquid from leaking from the upper mechanism and burning the electrical components below.

[0057] The shaking mechanism 200 is arranged on the processing platform 2. Specifically, the incubation tray rack 210 is installed on the top of the processing platform 2, and the motor 221 of the driving structure 220 is installed on the top of the processing platform 2, wherein a connecting seat is also arranged at the bottom of the processing platform 2, and the transmission shaft 224 passes through the connecting seat and can rotate relative to the connecting seat. The setting of the connecting seat increases the stability of the driving structure 220 fixed at the bottom of the processing platform 2. The processing platform 2 is also provided with a perforation, and the first fixing seat 2261 and the second fixing seat 2262 of the cam follower 226 are fixed to the bottom of the incubation tray rack 210 through the perforation, and the circulation pipe 350 of the drainage mechanism 400 also passes through the perforation. The perforations of the first fixing seat 2261 and the second fixing seat 2262 are much larger than the perforations of the circulation pipe 350, and the perforations of the circulation pipe 350 are preferably circular to fit the shape of the pipe.

[0058] The support frame 1 is arranged on the top of the processing platform 2, the liquid adding mechanism 300 is arranged on the support frame 1, the detergent tank 320 is arranged inside the space enclosed by the support frame 1, and the antibody tube 310, the solenoid valve tube clamp 330 and the heating peristaltic pump are installed on the outer wall of the support frame 1.

[0059] Optionally, the shaking mechanism 200 of the utility model can simultaneously control eight incubation trays 100 for protein blotting, which are arranged in two groups relative to each other. In addition to the detergent sink 320, it is equipped with eight groups of liquid adding mechanisms 300, but the detergent sink 320 of the liquid adding mechanism 300 is only one. In addition to the waste liquid tank, it is equipped with eight groups of drainage mechanisms 400. Two groups of slide rails 231 are provided, which are arranged at both ends of the incubation tray rack 210 and are arranged along the width direction of the incubation tray rack 210. At the same time, two groups of cams 225 and cam followers 226 are provided, which are arranged at a preset distance to ensure that each incubation tray 100 is fully shaken.

[0060] It should be noted that the number of incubation trays 100 is not limited to eight, but can also be an even number such as four, six, ten, twelve, etc. The corresponding supporting mechanisms can also be determined according to the specific number, and are not limited to the number described above.

[0061] The specific working process of this utility model:

[0062] 1. The control mechanism 500 starts to work, pours the blocking solution into the incubation tray 100, and then puts the experimental membrane into it for immersion and blocking.

[0063] 2. After the sealing is completed, the solenoid valve tube clamp 330 and the peristaltic pump 410 of the liquid discharge mechanism 400 are opened to discharge the sealing liquid; at the same time, the peristaltic pump 340 of the liquid adding mechanism 300 extracts the detergent in the detergent tank 320 into the incubation tray 100, and the shaking mechanism 200 starts to work, swinging left and right to rinse the experimental membrane, and rinses it three times.

[0064] 3. Then the liquid discharge mechanism 400 continues to work to discharge the detergent. After the detergent is completely discharged, the solenoid valve tube clamp 330 corresponding to the first antibody tube of the liquid adding mechanism 300 adds the first antibody reagent to the incubation tray 100, and the shaking mechanism 200 shakes the incubation tray 100 for incubation.

[0065] 4. After the incubation is completed, the discharge mechanism 400 discharges the primary antibody reagent, the liquid adding mechanism 300 adds detergent into the incubation tray 100, and the shaking mechanism 200 operates to rinse the experimental membrane.

[0066] 5. Then, the detergent is drained, the solenoid valve tube clamp 330 corresponding to the second antibody tube of the liquid adding mechanism 300 is opened, the second antibody reagent is added to the incubation tray 100, and the shaking mechanism 200 is operated to shake the incubation tray 100 for incubation.

[0067] 6. After the incubation is completed, the liquid adding mechanism 300 continues to add detergent to the incubation tray 100 and swings it left and right to rinse three times.

[0068] 7. Remove the experimental film.

[0069] In addition, those skilled in the art should understand that, although there are many problems in the prior art, each embodiment or technical solution of the utility model can be improved in only one or several aspects, without having to solve all the technical problems listed in the prior art or background technology at the same time. Those skilled in the art should understand that the content not mentioned in a claim should not be used as a limitation on the claim.

[0070] Although the terms such as support frame, processing platform, chassis, incubation tray, solution part, extension part, positioning part, shaking mechanism, incubation tray rack, driving structure, motor, synchronous wheel, synchronous belt, transmission shaft, cam, cam follower, first fixed seat, second fixed seat, limit block, sliding structure, slide rail, slider, liquid adding mechanism, antibody tube, detergent sink, solenoid valve pipe clamp, liquid adding peristaltic pump, circulation pipeline, liquid discharge mechanism, liquid discharge peristaltic pump, control mechanism, controller, display screen, heat dissipation mechanism, installation bin, fan and through hole are used more in this article, the possibility of using other terms is not excluded. The use of these terms is only to more conveniently describe and explain the essence of the utility model; interpreting them as any additional restrictions is contrary to the spirit of the utility model; the terms "first", "second", etc. (if any) in the specification and claims of the embodiments of the utility model and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence.

[0071] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the utility model, rather than to limit it. Although the utility model has been described in detail with reference to the aforementioned embodiments, ordinary technicians in this field should understand that they can still modify the technical solutions recorded in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not make the essence of the corresponding technical solution deviate from the scope of the technical solution of the embodiments of the utility model.

Claims

1. A protein immunoblotting device, characterized in that: include Incubation tray; A shaking mechanism, wherein the incubation tray is installed on the top of the shaking mechanism; A processing platform, wherein the shaking mechanism is fixed to the processing platform; A liquid adding mechanism, located on the top of the incubation tray, for adding reagents and lotions to the incubation device; A liquid discharge mechanism, located at the bottom of the processing platform and connected to the incubation tray through a flow channel; A control mechanism, the control mechanism being electrically connected to the shaking mechanism, the liquid adding mechanism and the liquid discharging mechanism; The shaking mechanism includes an incubation tray rack, a driving structure and a sliding structure. The incubation tray is installed on the incubation tray rack. The driving structure and the sliding structure are installed at the bottom of the incubation tray rack. The driving structure drives the incubation tray rack to slide horizontally on the upper part of the sliding structure.

2. The protein immunoblotting device according to claim 1, characterized in that: The incubation tray comprises a solution portion, an extension portion and a retaining member, wherein the extension portion is arranged on the top side of the solution portion and is perpendicular to the side of the solution portion; the retaining member is arranged on the outside of the extension portion and is clamped under the incubation tray rack.

3. The protein immunoblotting device according to claim 1, characterized in that: The driving structure includes a motor, a synchronous wheel, a synchronous belt, a transmission shaft, a cam and a cam follower. The motor drives the synchronous wheel and the synchronous belt to drive the synchronous belt and the synchronous wheel. The transmission shaft passes through the synchronous wheel and the cam, and the transmission shaft is eccentrically installed on the cam to drive the cam to rotate.

4. The protein immunoblotting device according to claim 3, characterized in that: The cam follower comprises a first fixing seat and a second fixing seat, wherein the first fixing seat and the second fixing seat are mounted on the bottom of the incubation tray rack, and the first fixing seat and the second fixing seat are provided with a limiting block opposite to each other, the limiting block is arranged perpendicular to the fixing seat, and the side edge of the cam abuts against the limiting block; The installation point of the transmission shaft on the cam coincides with the center of the line between the limiting blocks of the first fixing seat and the second fixing seat, and the center of the cam does not coincide with the center of the line between the limiting blocks.

5. The protein immunoblotting device according to claim 1, characterized in that: The sliding structure comprises a slide rail and a slider, wherein the slider is mounted on the bottom of the incubation tray rack, the slide rail is fixed on the top of the processing platform, and the slider slides linearly back and forth relative to the slide rail.

6. The protein immunoblotting device according to claim 1, characterized in that: The liquid adding mechanism comprises an antibody tube, a detergent sink, an electromagnetic valve tube clamp and a liquid adding peristaltic pump. The electromagnetic valve tube clamp is arranged on a flow pipe at the bottom of the antibody tube, and the liquid adding peristaltic pump is installed on the detergent sink.

7. The protein immunoblotting device according to claim 1, characterized in that: The drainage mechanism includes a drainage peristaltic pump, a solenoid valve pipe clamp and a waste liquid pool. The bottom of the incubation plate is provided with an opening, which is connected to the solenoid valve pipe clamp and the drainage peristaltic pump through a circulation pipeline to discharge the waste liquid into the waste liquid pool.

8. The protein immunoblotting device according to claim 1, characterized in that: The protein immunoblotting device is also provided with a machine case, the processing platform is arranged on the top of the machine case, and the liquid discharge mechanism and the driving structure are arranged inside the machine case.

9. The protein immunoblotting device according to claim 1, characterized in that: It also includes a heat dissipation mechanism, which is arranged inside the chassis and opposite to the driving structure; The heat dissipation mechanism comprises an installation chamber and a fan. The installation chamber is fixedly installed on the chassis base, the fan is installed in the inner cavity of the installation chamber, and the surface of the installation chamber is provided with through holes for air circulation.

10. The protein immunoblotting device according to claim 9, characterized in that: The control mechanism comprises a controller and a display screen, and the controller is electrically connected to the display screen, the driving structure, the liquid adding mechanism, and the liquid discharging mechanism.

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