A cell culture incubator to avoid contamination

Abstract

The utility model discloses a cell culture box for avoiding contamination, wherein the rotating mechanism comprises a motor, a ratchet and a first connecting rod, the motor is fixedly mounted on the bottom of the box, the output end of the motor passes through the bottom of the box and is fixedly connected with a second connecting rod, the first connecting rod is rotatably connected with the second connecting rod, the top of the first connecting rod is rotatably connected with a third connecting rod, the top of the ratchet is fixedly connected to the bottom of a culture plate, and the bottom of the ratchet is fixedly connected with a rotating shaft, and relates to the technical field of cell culture devices, the second connecting rod is rotated by the rotation of the motor, and the ratchet is driven to rotate in coordination with the first connecting rod, the third connecting rod and a pushing component, so that the culture plate and the culture dish in the box are rotated to a certain angle, so as to bring the object to be observed closer for better observation, and at the same time, the angle can be better adjusted to add the required solution to the culture dish that needs to be added with liquid.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture devices, in particular to a cell culture box capable of avoiding contamination. Background Art

[0002] After searching, the Chinese patent publication number is CN213951221U, and the publication date is 2021-08-13. It discloses a cell culture device for experiments that is easy to extract, including a culture box body, a rubber telescopic glove is fixedly connected to the top of the culture box body, and the left and right side walls of the culture box body are fixedly connected to fixed bars, and the inner sides of the two fixed bars are slidably connected to sliding plates, and a culture plate is fixedly connected between the opposite sides of the two sliding plates, and the top of the culture plate is movably connected to a culture box, and the right side of the culture box is fixedly connected to an extraction box, and the right side of the extraction box is movably connected to a movable door. The culture device for cell experiments that is easy to extract has a simple overall structure and achieves the purpose of making the culture device easy to extract. When medical personnel need to extract cells, they do not need to directly take them from the inside of the culture device, which avoids contamination of the cells, increases the life of the cells, and improves the efficiency and effect of the use of the culture device. However, during the cell culture process, it is often necessary to observe the state of the cells at a close distance, and the multiple groups of culture dishes in the culture device are fixed in position and can be changed to bring the viewing angle closer to facilitate observation by experimenters. At the same time, during the cell culture process, it is necessary to add multiple solutions such as serum and buffer to maintain the growth and division of the cells, but the device does not have this function. Utility Model Content

[0003] The purpose of the utility model is to provide a cell culture box that avoids contamination and solves the technical problems mentioned in the above background technology.

[0004] The purpose of the utility model can be achieved through the following technical solutions:

[0005] A cell culture box for avoiding contamination comprises a box body and a culture plate. A liquid adding port is provided on the top of the box body. A rotating mechanism is movably connected between the box body and the culture plate. A liquid adding mechanism is arranged on the top of the box body.

[0006] The rotating mechanism includes a motor, a ratchet and a first connecting rod. The motor is fixedly installed at the bottom of the box, the output end of the motor passes through the bottom of the box and is fixedly connected to the second connecting rod, the first connecting rod is rotatably connected to the second connecting rod, the top of the first connecting rod is rotatably connected to a third connecting rod, the top of the ratchet is fixedly connected to the bottom of the culture plate, the bottom of the ratchet is fixedly connected to a rotating shaft, the third connecting rod and one end of the rotating shaft are rotatably connected in the box, and the top of the third connecting rod is movably connected to a pushing component.

[0007] As a further solution of the utility model: the pushing assembly includes a pawl and a fixed plate, the bottom of the pawl is fixedly connected to a pawl shaft, one end of the pawl shaft is rotatably connected to the top of the third connecting rod, the pawl is engaged with the ratchet wheel, the bottom of the fixed plate is fixedly connected to the top of the third connecting rod, and a first spring is fixedly connected between the pawl and the fixed plate.

[0008] As a further solution of the utility model: the liquid adding mechanism includes an installation box, a cylinder and a measuring cylinder, the top of the installation box is movably connected with a cover, the bottom of the installation box is penetrated with a liquid injection hole, the cylinder is fixedly installed on the inner wall of the installation box, and multiple cylinders and liquid injection holes are symmetrically arranged along the center of the installation box, the movable end of the cylinder is fixedly connected with a piston, the measuring cylinder is threadedly connected in the liquid injection hole, the bottom of the measuring cylinder is fixedly connected with a liquid injection tube, the liquid injection tube is located in the liquid adding port, the piston is slidably connected in the measuring cylinder, and a snap assembly is movably connected between the installation box and the box body, and two snap assemblies are symmetrically arranged along the center of the installation box.

[0009] As a further solution of the utility model: the buckle assembly includes a slot box and a buckle box, the slot box is fixedly connected to the top of the box body, the buckle box is fixedly connected to the outer wall of the installation box, a push button is slidably connected in the groove on the top of the buckle box, a slide groove is penetrated on the buckle box, a card column is fixedly connected to the bottom of the push button, one end of the card column penetrates the slide groove and extends into the slot box, a second spring is fixedly connected between the card column and the installation box, and the second spring is located below the slide groove.

[0010] As a further solution of the utility model: the clamping column is slidably connected to the sliding slot, and the clamping slot box is slidably connected to the buckle box.

[0011] As a further solution of the utility model: a transparent window and a sealed door are fixedly connected to the box body, and an operation port is opened through the top of the box body. There are two operation ports, and rubber gloves are fixedly connected to the two operation ports.

[0012] Beneficial Effects

[0013] The utility model provides a cell culture box that avoids contamination. Compared with the prior art, it has the following beneficial effects:

[0014] (1) The present application rotates the second connecting rod by the rotation of the motor, and cooperates with the first connecting rod, the third connecting rod and the pushing assembly to drive the ratchet to rotate, so as to rotate the culture plate and the culture dish in the box to a certain angle, so as to bring the object to be observed closer for better observation, and at the same time, the angle can be better adjusted to add the required solution to the culture dish that needs to be added with liquid.

[0015] (2) The present application injects liquid into the culture dish through a cylinder, a measuring cylinder and an injection tube to keep the cells active during the culture process. The snap-fit ​​assembly ensures the fixation and sealing of the connection between the installation box and the box body, thereby ensuring a sterile environment inside the box body. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a three-dimensional diagram of the utility model;

[0017] Figure 2 It is the main cross-sectional view of the utility model;

[0018] Figure 3 It is a three-dimensional diagram of the rotating mechanism and the culture plate of the utility model;

[0019] Figure 4 It is a top sectional view of the rotating mechanism of the utility model;

[0020] Figure 5 For this utility model Figure 2 A partial enlarged view of the middle A;

[0021] Figure 6 It is an exploded view of the buckle assembly of the utility model.

[0022] In the figure: 1. box body; 2. culture plate; 3. liquid filling port; 4. rotating mechanism; 41. motor; 42. ratchet; 43. first connecting rod; 44. second connecting rod; 45. third connecting rod; 46. rotating shaft; 47. pushing assembly; 471. ratchet; 472. fixing plate; 473. claw shaft; 474. first spring; 5. liquid filling mechanism; 51. installation box; 52. cylinder; 53. measuring cylinder; 54. cover; 55. liquid injection hole; 56. piston; 57. liquid injection tube; 58. buckle assembly; 581. card slot box; 582. card buckle box; 583. push button; 584. slide slot; 585. card column; 586. second spring; 6. transparent window; 7. sealing door; 8. operation port; 9. rubber gloves. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] See also Figure 1-6As shown, the utility model is a cell culture box for avoiding contamination, comprising a box body 1 and a culture plate 2, a liquid adding port 3 is opened through the top of the box body 1, a rotating mechanism 4 is movably connected between the box body 1 and the culture plate 2, and a liquid adding mechanism 5 is arranged on the top of the box body 1; the rotating mechanism 4 comprises a motor 41, a ratchet 42 and a first connecting rod 43, the motor 41 is fixedly installed at the bottom of the box body 1, the output end of the motor 41 passes through the bottom of the box body 1 and is fixedly connected to the second connecting rod 44, the first connecting rod 43 is rotatably connected to the second connecting rod 44, the top of the first connecting rod 43 is rotatably connected to the third connecting rod 45, the top of the ratchet 42 is fixedly connected to the bottom of the culture plate 2, the bottom of the ratchet 42 is fixedly connected to a rotating shaft 46, the third connecting rod 45 and one end of the rotating shaft 46 are rotatably connected in the box body 1, and the top of the third connecting rod 45 is movably connected to a pushing component 47.

[0025] The pushing assembly 47 includes a pawl 471 and a fixed plate 472. The bottom of the pawl 471 is fixedly connected to a pawl shaft 473. One end of the pawl shaft 473 is rotatably connected to the top of the third connecting rod 45. The pawl 471 is engaged with the ratchet wheel 42. The bottom of the fixed plate 472 is fixedly connected to the top of the third connecting rod 45. A first spring 474 is fixedly connected between the pawl 471 and the fixed plate 472.

[0026] The input end of the motor 41 is connected to an external power supply. When the motor 41 rotates, the second connecting rod 44 rotates, and cooperates with the first connecting rod 41, the third connecting rod 45 and the pushing assembly 47 to drive the ratchet 42 to rotate, so as to rotate the culture plate 2 and the culture dish in the box 1 to a certain angle, so as to bring the object to be observed closer for better observation, and at the same time, the angle can be better adjusted to add the required solution to the culture dish that needs to be added with liquid.

[0027] The liquid adding mechanism 5 includes an installation box 51, a cylinder 52 and a measuring cylinder 53. A cover 54 is movably connected to the top of the installation box 51. A liquid injection hole 55 is opened through the bottom of the installation box 51. The cylinder 52 is fixedly installed on the inner wall of the installation box 51. There are multiple cylinders 52 and liquid injection holes 55 symmetrically arranged along the center of the installation box 51. A piston 56 is fixedly connected to the movable end of the cylinder 52. The measuring cylinder 53 is threadedly connected in the liquid injection hole 55. A liquid injection tube 57 is fixedly connected to the bottom of the measuring cylinder 53. The liquid injection tube 57 is located in the liquid adding port 3. The piston 56 is slidably connected in the measuring cylinder 53. A snap assembly 58 is movably connected between the installation box 51 and the box body 1. Two snap assemblies 58 are symmetrically arranged along the center of the installation box 51.

[0028] The buckle assembly 58 includes a slot box 581 and a buckle box 582. The slot box 581 is fixedly connected to the top of the box body 1, and the buckle box 582 is fixedly connected to the outer wall of the installation box 51. A push button 583 is slidably connected in the groove on the top of the buckle box 582. A slide groove 584 is penetrated through the buckle box 582. A card column 585 is fixedly connected to the bottom of the push button 583. One end of the card column 585 penetrates the slide groove 584 and extends into the slot box 581. A second spring 586 is fixedly connected between the card column 585 and the installation box 51, and the second spring 586 is located below the slide groove 584.

[0029] The clamping column 585 is slidably connected to the sliding groove 584 , and the clamping slot box 581 is slidably connected to the buckle box 582 .

[0030] The input end of the cylinder 52 is connected to an external power source. The cylinder 52, the measuring cylinder 53 and the injection tube 57 inject liquid into the culture dish to keep the cells active during the culture process. The snap assembly 58 ensures the fixation and sealing of the connection between the installation box 51 and the box body 1, ensuring the sterile environment inside the box body.

[0031] A transparent window 6 and a sealed door 7 are fixedly connected to the box body 1 , and an operation port 8 is provided through the top of the box body 1 . There are two operation ports 8 , and rubber gloves 9 are fixedly connected to the two operation ports 8 .

[0032] Meanwhile, the contents not described in detail in this specification belong to the prior art known to those skilled in the art.

[0033] The working principle of the utility model is as follows: the installation box 51 is placed on the box body 1 so that the injection tube 57 is located in the liquid filling port 3, and the two push buttons 583 are pushed to move in the direction close to the installation box 51 so that the clamping column 585 is located in the shorter groove of the clamping slot box 581, and the installation box 51 is rotated to move the clamping column 585 to the longer groove of the clamping slot box 581, and the second spring 586 is restored to its original length so that the installation box 51 is tightly installed on the top of the box body 1. When the culture plate 2 needs to be rotated, the power supply of the motor 41 is turned on, and the motor 41 is driven by the power supply. The second connecting rod 44 is driven to rotate, thereby driving the first connecting rod 43 to rotate, and the third connecting rod 45 swings accordingly, thereby driving the pawl 471 to move back and forth and engage with the ratchet 42 under the thrust of the fixed plate 472 and the first spring 474, pushing the ratchet 42 to rotate a certain angle, thereby achieving the purpose of rotating the culture plate 2 to change the observation object. When it is necessary to inject solution into the culture dish, the power supply of the cylinder 52 is turned on, and the moving end of the cylinder 52 pushes the piston 56 downward to allow the solution in the measuring cylinder 53 to enter the culture dish through the injection tube.

[0034] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0035] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell culture box for avoiding contamination, comprising a box body (1) and a culture plate (2), characterized in that: A liquid adding port (3) is provided on the top of the box (1), a rotating mechanism (4) is movably connected between the box (1) and the culture plate (2), and a liquid adding mechanism (5) is provided on the top of the box (1); The rotating mechanism (4) comprises a motor (41), a ratchet (42) and a first connecting rod (43); the motor (41) is fixedly mounted at the bottom of the housing (1); the output end of the motor (41) passes through the bottom of the housing (1) and is fixedly connected to the second connecting rod (44); the first connecting rod (43) and the second connecting rod (44) are rotatably connected; a third connecting rod (45) is rotatably connected to the top of the first connecting rod (43); the top of the ratchet (42) is fixedly connected to the bottom of the culture plate (2); the bottom of the ratchet (42) is fixedly connected to a rotating shaft (46); one end of the third connecting rod (45) and the rotating shaft (46) are rotatably connected in the housing (1); and the top of the third connecting rod (45) is movably connected to a pushing assembly (47).

2. A cell culture incubator for avoiding contamination according to claim 1, characterized in that: The pushing assembly (47) includes a pawl (471) and a fixed plate (472), the bottom of the pawl (471) is fixedly connected to a pawl shaft (473), one end of the pawl shaft (473) is rotatably connected to the top of the third connecting rod (45), the pawl (471) is engaged with the ratchet wheel (42), the bottom of the fixed plate (472) is fixedly connected to the top of the third connecting rod (45), and a first spring (474) is fixedly connected between the pawl (471) and the fixed plate (472).

3. A cell culture incubator for avoiding contamination according to claim 1, characterized in that: The liquid adding mechanism (5) comprises an installation box (51), a cylinder (52) and a measuring cylinder (53); the top of the installation box (51) is movably connected to a cover (54); the bottom of the installation box (51) is provided with a liquid injection hole (55); the cylinder (52) is fixedly mounted on the inner wall of the installation box (51); a plurality of the cylinders (52) and the liquid injection holes (55) are symmetrically arranged along the center of the installation box (51); the movable end of the cylinder (52) is fixedly mounted on the inner wall of the installation box (51); The measuring cylinder (53) is fixedly connected with a piston (56), the measuring cylinder (53) is threadedly connected in the injection hole (55), the bottom of the measuring cylinder (53) is fixedly connected with an injection tube (57), the injection tube (57) is located in the liquid filling port (3), the piston (56) is slidably connected in the measuring cylinder (53), and a buckle assembly (58) is movably connected between the installation box (51) and the box body (1), and two buckle assemblies (58) are symmetrically arranged along the center of the installation box (51).

4. A cell culture incubator for avoiding contamination according to claim 3, characterized in that: The buckle assembly (58) comprises a slot box (581) and a buckle box (582), wherein the slot box (581) is fixedly connected to the top of the box body (1), and the buckle box (582) is fixedly connected to the outer wall of the installation box (51), a push button (583) is slidably connected in the groove at the top of the buckle box (582), a slide groove (584) is penetrated through the buckle box (582), a clamping column (585) is fixedly connected to the bottom of the push button (583), one end of the clamping column (585) penetrates the slide groove (584) and extends into the slot box (581), and a second spring (586) is fixedly connected between the clamping column (585) and the installation box (51), and the second spring (586) is located below the slide groove (584).

5. A cell culture incubator for avoiding contamination according to claim 4, characterized in that: The clamping column (585) is slidably connected to the sliding groove (584), and the clamping slot box (581) is slidably connected to the buckle box (582).

6. A cell culture incubator for avoiding contamination according to claim 1, characterized in that: The box body (1) is respectively fixedly connected with a transparent window (6) and a sealed door (7); the top of the box body (1) is penetrated with an operating port (8); two operating ports (8) are provided, and rubber gloves (9) are fixedly connected in the two operating ports (8).

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