An automatic algae sampling device

Abstract

The utility model relates to the technical field of phytoplankton physiological and ecological engineering, and in particular to an automatic algae sampling device. An automatic algae sampling device comprises a chassis and a circulation counting pool, wherein a cleaning and sampling module and a sampling module are arranged in the chassis, wherein a sample rack is arranged on the cleaning and sampling module, wherein a plurality of water sample bottles, pure water bottles, and detergent bottles are sequentially arranged on the sample rack, wherein a hollow sampling hollow needle is arranged on the sampling module, and the sampling hollow needle is tightly connected to the circulation counting pool through a sampling tube. Through the device and method of the utility model, an automatic liquid homogenizing device combining a washing liquid pool for washing, a pure water pool for washing, and a multi-water sample pool can be automatically controlled to complete the corresponding entry of a plurality of algae water samples into the microscopic photography area, and after the photography is completed, washing liquid is automatically extracted for washing, and then the photography area and the sampling pipeline are automatically cleaned with pure water, and then the next batch of algae water samples are automatically sampled.

Description

Technical Field

[0001] The utility model relates to the technical field of phytoplankton physiological and ecological engineering, in particular to an algae automatic sampling device. Background Art

[0002] Traditional algae detection devices, such as Chinese patent ZL200910305949.1, are mainly used in environmental protection, tap water and water conservancy industries to detect and evaluate the hazards of algae in water bodies. They use automatic classification and concentration calculation technology based on image analysis to replace traditional manual naked eye identification technology, greatly reducing the workload of algae detection and shortening the detection time. However, due to its single function and mostly relying on manual operation, it has many defects.

[0003] Therefore, in response to the problems existing in the above-mentioned prior art, Chinese patent CN102564913B discloses the following technical solutions: a sampling device for algae detection, comprising a water inlet channel and a bracket, a cover plate is provided on the top of the water inlet channel, a telescopic top column that can perform up and down telescopic movement is provided on the bottom of the water inlet channel, the top end of the telescopic top column is abutted or hinged to one end of the cover plate, the other end of the cover plate is hinged to the bracket, and a fixed support platform for receiving the cover plate is provided at the bottom of the water inlet channel.

[0004] The above-mentioned prior art simplifies the detection device in the prior art, reduces the accuracy requirement, and reduces the cost. However, the reduced accuracy caused by the simplification of the above-mentioned prior art is not conducive to the existing high-precision and automated detection requirements. Utility Model Content

[0005] The utility model provides an algae automatic sampling device that can realize multi-channel and multi-sample automatic sampling, can shake and mix algae samples, can effectively clean the pipeline to prevent cross contamination of water samples, and can effectively prevent the generation of bubbles. In order to optimize the above technical problems, the utility model provides the following technical solutions:

[0006] An algae automatic sampling device comprises a chassis and a circulation counting pool. A cleaning and sampling module and a sampling module are arranged in the chassis. A sample rack is arranged on the sampling module. A plurality of water sample bottles, pure water bottles and detergent bottles are placed on the sample rack. The sampling module is provided with a sampling hollow needle. The sampling hollow needle is tightly connected to the circulation counting pool through a sampling tube. The sampling hollow needle samples in the water sample bottle and transports the sampled liquid to the circulation counting pool through the sampling tube for sample photography and analysis.

[0007] On the basis of adopting the above technical solution, the utility model can also adopt the following further technical solution:

[0008] The sample rack comprises a sample area for placing water sample bottles and at least two boxes, wherein the boxes are filled with pure water or cleaning liquid, and sampling holes are opened at the upper end of the boxes, and the number of the sampling holes corresponds to the number of the sampling hollow needles.

[0009] The injection module also includes a sample placement plate, a first slider and a horizontal guide rail. The first slider is arranged below the sample placement plate and is slidably connected to the horizontal guide rail. The sample placement plate is also provided with a first motor that is connected to an external power supply. When the first motor is powered on, it drives the sample placement plate to move horizontally at the horizontal guide rail.

[0010] The sampling module also includes a pipeline connecting joint, a second slider and a vertical guide rail. The second slider is arranged on the side of the sampling hollow needle, and the second slider is slidably connected to the vertical guide rail. The second slider is connected to a second motor that is connected to an external power supply. When the second motor is energized, it drives the second slider to drive the sampling hollow needle to move up and down on the vertical guide rail. The pipeline connecting joint is connected to the sampling tube.

[0011] A multi-channel peristaltic pump is also provided at the bottom of the chassis. The sampling tube is connected to the circulation counting pool after passing through the multi-channel peristaltic pump after being connected from the sampling module.

[0012] The front of the chassis is provided with an opening door, the top of the chassis is provided with a switch, the chassis is provided with an RS485 interface and the chassis is electrically connected to a computer host computer through a wire.

[0013] The circulation counting pool is provided with a multi-channel algae counting frame, and a single algae counting frame includes a highly transparent bottom plate, a diaphragm fence, a cover plate and a pressure plate. The diaphragm fence is in an enclosed structure and is hollow to form a volume pool. The highly transparent cover plate is arranged above the volume pool. The single algae counting frame is also provided with an inlet channel and an outlet channel, and the inlet channel and the outlet channel are both connected to the volume pool. The inlet channel is connected to the sampling module through a sampling tube, and the outlet channel is connected to the waste liquid barrel through the sampling tube.

[0014] The fence is made of a silicone mold, and the cover glass is made of a highly transparent and high-strength thin material. The silicone membrane is used as the fence of the algae counting frame volume pool, which has corrosion resistance and leakage prevention characteristics. The cover glass of the algae counting frame is made of a highly transparent and high-strength thin material, which is convenient for installation on the microscope platform and easy to replace and disassemble.

[0015] Compared with the prior art, the utility model can achieve the following beneficial effects:

[0016] The device and method of the utility model combines the automatic liquid homogenization device of the washing liquid pool for cleaning, the pure water pool for cleaning, and the multi-water sample pool, which can automatically control the completion of a single or multiple water sample observation and photography area, automatically extract the washing liquid for cleaning after the photography is completed, and then automatically use pure water to clean the photography area and the sampling pipeline, and then automatically sample the next batch of algae water samples. The sampling device can effectively avoid the generation of bubbles, can effectively clean the pipeline to avoid sample residue, and has reasonable control of the flow rate and the amount of liquid taken, so as to achieve the shaking and homogenization of the sample and reduce the sampling error. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 The utility model is a schematic diagram of the structure of an automatic algae sampling device.

[0018] Figure 2 The utility model is a schematic diagram of the internal structure of an automatic algae sampling device.

[0019] Figure 3 The utility model is a schematic diagram of the structure of a circulation counting pool of an algae automatic sampling device.

[0020] Among them, there are a chassis 1, a circulation counting pool 2, an algae counting frame 21, a fence 22, a volume pool 23, a sample inlet channel 24, a sample outlet channel 25, a sample rack 31, a water sample bottle 32, a box body 33, a sampling hole 34, a sample placement plate 35, a first slider 36, a horizontal guide rail 37, a sampling hollow needle 41, a pipeline connecting joint 42, a second slider 43, a vertical guide rail 44, a first photoelectric switch 45, a sampling tube 5, and a sample outlet tube 6. DETAILED DESCRIPTION

[0021] In conjunction with the accompanying drawings, the automatic algae sampling device provided by the utility model is further described.

[0022] Examples, such as Figure 1-3 As shown, an algae automatic sampling device comprises a chassis 1 and a circulation counting pool 2, wherein the chassis 1 is provided with a sampling module and a sampling module.

[0023] Furthermore, a peristaltic pump is provided at the bottom of the chassis 1 , and the sampling tube 5 is connected to the circulation counting pool 2 or the waste liquid barrel after being connected from the sampling module through the peristaltic pump.

[0024] Furthermore, the front of the chassis 1 is provided with an opening door, the top of the chassis 1 is provided with a switch, the chassis is provided with an RS485 interface and the chassis is electrically connected to a computer host computer through a wire, so as to control the sample injection device through the computer host computer.

[0025] Furthermore, the circulation counting pool 2 is provided with a plurality of algae counting frames 21 , and a single algae counting frame includes a fence 22 and a cover glass. The fence 22 is an enclosed structure and is hollow to form a volume pool 23 .

[0026] Furthermore, a cover glass is arranged above the volume pool 23 , and a single algae counting frame 21 is also provided with a sample inlet channel 24 and a sample outlet channel 25 , and both the sample inlet channel 24 and the sample outlet channel 25 are connected to the volume pool 23 .

[0027] Furthermore, the sampling channel 24 is connected to the sampling module through the sampling tube 5, and the sampling channel 25 is connected to the waste liquid bucket through the sampling tube 6 for sampling.

[0028] Furthermore, the fence 22 is made of a silicone mold, and the cover glass is made of a thin material with high transparency and high strength.

[0029] Specifically, a sample rack 31 is provided on the sample injection module, and a plurality of sample bottles 32 are placed on the sample rack 31 .

[0030] Furthermore, the sample rack 31 includes a sample area for placing a sample bottle and at least two boxes 33, in which pure water or cleaning liquid can be installed as needed. The box 33 is provided with sampling holes 34, and the number of the sampling holes 34 corresponds to the number of the sampling hollow needles 41.

[0031] Furthermore, the sample injection module further includes a sample placement plate 35 , a first slider 36 and a horizontal guide rail 37 . The first slider 36 is disposed below the sample placement plate 35 , and the first slider 35 is slidably connected to the horizontal guide rail 37 .

[0032] Furthermore, a first motor connected to an external power source is provided on the sample placement plate 35 . When the first motor is powered on, it drives the sample placement plate 35 to move laterally at the horizontal guide rail 37 .

[0033] Furthermore, the sampling module is provided with a sampling hollow needle 41, which is connected to the circulation counting pool 2 through the sampling tube 5. The sampling hollow needle 41 samples in the sample bottle 32 and transports the sampling liquid to the circulation counting pool 2 through the sampling tube 5 for sample analysis.

[0034] Furthermore, the sampling module also includes a pipeline connecting joint 42 , a second slider 43 and a vertical guide rail 44 . The second slider 43 is arranged on the side of the sampling hollow needle 41 , and the second slider 43 is slidably connected to the vertical guide rail 44 .

[0035] Furthermore, the second slider 43 is connected to a second motor connected to an external power source. When the second motor is powered on, it drives the second slider 43 to drive the sampling hollow needle 41 to move up and down on the vertical guide rail 44.

[0036] Furthermore, the pipeline connection joint 42 is connected to the sampling tube 5 .

[0037] Furthermore, a first photoelectric switch 45 is provided on the side of the pipeline connection joint 42, and a second photoelectric switch is provided on the rear of the chassis 1. The two photoelectric switches are used to control the motor to return to zero and determine the position of the slider.

[0038] Specifically, the algae automatic sampling method in this embodiment includes the following steps:

[0039] S1. Place the water sample bottle, pure water bottle, and detergent bottle containing samples in the sample rack, and fix the flow counting cell on the observation platform of the microscope;

[0040] S2, turn on the device switch, start the first motor to control the sample placement plate rack to move back and forth quickly to shake the water sample;

[0041] S3, after the sample is fully shaken, start the first motor to move the sample placement plate rack until the sample bottle is located below the sampling hollow needle;

[0042] S4, start the second motor to move the sampling hollow needle downward until it is inserted into the sample bottle;

[0043] S5, after controlling the multi-channel peristaltic pump to run at a low speed to extract a quantitative sample, the second motor is started to move the sampling hollow needle upward until it is completely out of the sample bottle;

[0044] S6, controlling the peristaltic pump to run at a medium speed to inject the sample into the flow-through counting cell;

[0045] S7. Observe and analyze the samples in the flow counting cell using a microscope;

[0046] S8. After the sample analysis is completed, control the peristaltic pump to run at full speed to empty the pipeline.

[0047] Among them, the circulation counting pool 2 is fixed on the observation platform of the microscope in step S1. After completing steps S1-S8, if it is necessary to observe the next sample, there is no need to repeat step S1. If the samples have been placed in different water sample bottles, the injection can be started from step S3 until step S8. If all the samples have been injected and observed, the injection can be started from step S2 until step S8.

[0048] The microscope used in the above method is existing technology, and has functions such as supporting 40X, 20X, 10X and other objective lens imaging, image resolution can be as high as ≤0.125μm / pixel, high-precision electric-controlled XYZ microscope automatic scanning platform with X / Y axis repeat positioning accuracy <2μm (scanning platform resolution ≤0.05μm), multi-depth of field fusion continuous automatic scanning characteristics, commercially available microscopes or electric XYZ microscope automatic platform products with adjustable shooting layers and layer spacing, etc.

[0049] The cleaning method of the sample injection device in this embodiment comprises the following steps:

[0050] S11, starting the first motor to move the sample placement plate until the box containing the cleaning solution or pure water is located under the sampling hollow needle;

[0051] S12, starting the second motor to move the sampling hollow needle downward until it extends into the cleaning liquid box body;

[0052] S13, control the peristaltic pump to run at full speed and clean the pipeline multiple times at a high flow rate.

[0053] The present invention has been described with reference to the preferred embodiments; however, it will be understood by those skilled in the art that various changes in form and details may be made within the scope of the claims.

Claims

1. An algae automatic sampling device, characterized in that It includes a chassis and a circulation counting pool. The chassis is provided with a cleaning and sampling module and a sampling module. The sampling module is provided with a sample rack, on which a plurality of water sample bottles, pure water bottles and detergent bottles are placed. The sampling module is provided with a sampling hollow needle, which is tightly connected to the circulation counting pool through a sampling tube. The sampling hollow needle takes samples in the water sample bottle and transports the sampled liquid to the circulation counting pool through the sampling tube for sample photography and analysis.

2. The algae automatic sampling device according to claim 1, characterized in that The sample rack comprises a sample area for placing water sample bottles and at least two boxes, wherein the boxes are filled with pure water and cleaning liquid, and the upper end of the boxes is provided with sampling holes, the number of which corresponds to the number of sampling hollow needles.

3. The algae automatic sampling device according to claim 1, characterized in that The injection module also includes a sample placement plate, a first slider and a horizontal guide rail. The first slider is arranged below the sample placement plate rack, and the first slider is slidably connected to the horizontal guide rail. The sample placement plate rack is also provided with a first motor that is connected to an external power supply. When the first motor is powered on, it drives the sample placement plate rack to move horizontally at the horizontal guide rail.

4. The algae automatic sampling device according to claim 1, characterized in that The sampling module also includes a pipeline connecting joint, a second slider and a vertical guide rail. The second slider is arranged on the side of the sampling hollow needle, and the second slider is slidably connected to the vertical guide rail. The second slider is connected to a second motor that is connected to an external power supply. When the second motor is energized, it drives the second slider to drive the sampling hollow needle to move up and down on the vertical guide rail. The pipeline connecting joint is connected to the sampling tube.

5. The algae automatic sampling device according to claim 1, characterized in that A multi-channel peristaltic pump is also provided at the bottom of the chassis. The sampling tube is connected to the circulation counting pool after passing through the multi-channel peristaltic pump after being connected from the sampling module.

6. The algae automatic sampling device according to claim 1, characterized in that The front of the chassis is provided with an opening door, the top of the chassis is provided with a switch, the chassis is provided with an RS485 interface and the chassis is electrically connected to a computer host computer through a wire.

7. The algae automatic sampling device according to claim 1, characterized in that The circulation counting pool is provided with a multi-channel algae counting frame, and a single algae counting frame includes a highly transparent bottom plate, a diaphragm fence, a cover plate and a pressure plate. The diaphragm fence is in an enclosed structure and is hollow to form a volume pool. The highly transparent cover plate is arranged above the volume pool. The single algae counting frame is also provided with an inlet channel and an outlet channel, and the inlet channel and the outlet channel are both connected to the volume pool. The inlet channel is connected to the sampling module through a sampling tube, and the outlet channel is connected to the waste liquid barrel through the sampling tube.

8. The algae automatic sampling device according to claim 7, characterized in that The diaphragm fence is made of a silicone film, and the bottom plate, cover plate and pressure plate are made of a highly transparent and high-strength material.

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