Dark adaptation leaf clamp for chlorophyll fluorescence detection

Abstract

The utility model relates to the technical field of chlorophyll fluorescence determination, and discloses a dark adaptation leaf clamp for chlorophyll fluorescence detection, which comprises an upper clamping plate, a lower clamping plate and a shading plate, the top of the upper clamping plate is provided with an optical fiber guide hole column, and the top of the optical fiber guide hole column is provided with a through hole which extends downwards and penetrates through the bottom of the upper clamping plate. A slot penetrating through the cross section of the through hole is also formed in the outer side wall of the optical fiber guide hole column, and the shading plate is slidably mounted in the slot; a pressing plate is arranged on the outer side of the shading plate, guide columns are arranged at the two ends of the side, close to the optical fiber guide hole column, of the pressing plate, two mounting lugs are further formed on the outer side wall of the optical fiber guide hole column, guide holes in sliding fit with the guide columns are formed in the mounting lugs, and limiting pieces are formed at the ends of the guide columns. The utility model aims to prevent a blade from being damaged when the blade is clamped and prevent the accuracy of detection data from being influenced by light leakage.

Description

Technical Field

[0001] The utility model belongs to the technical field of chlorophyll fluorescence determination, and particularly relates to a dark-adapted leaf clip for chlorophyll fluorescence detection. Background Art

[0002] When plants are subjected to environmental stress, their photosynthetic characteristics will show different degrees of changes. It is the material basis of all material metabolism and energy metabolism in the biological world. It includes a series of complex processes of photophysics, photochemistry and biochemistry. In the process of photosynthesis, part of the light energy loss is released in the form of long fluorescence during the original reaction of photosynthesis, which transfers and converts the absorbed light energy into electrical energy. As a probe for photosynthesis research, chlorophyll fluorescence has been widely studied and applied. Chlorophyll fluorescence can not only reflect the primary reaction process of photosynthesis such as light energy absorption, excitation energy transfer and photochemical reaction, but also is related to processes such as electron transfer, proton gradient establishment, ATP synthesis and CO2 fixation. Almost all changes in the photosynthesis process can be reflected by chlorophyll fluorescence, and the fluorescence measurement technology does not need to break cells and does not harm organisms. Therefore, it is a simple, fast and reliable method to indirectly study the changes in photosynthesis by studying chlorophyll fluorescence. Chlorophyll fluorescence has been widely used in photosynthesis, plant stress physiology, aquatic biology, oceanography and remote sensing.

[0003] Existing dark-adaptive leaf clamps usually use two magnets to clamp the blades and the shading paper. For example, the patent with application number CN201920095168.3 discloses a low-cost dark-adaptive leaf clamp, including: two ferrules; each of the ferrules has a mounting slot, and a magnet is installed in the mounting slot; and the magnets in the two ferrules have opposite magnetic properties; the shading paper is fixed between the two ferrules through the magnet. When using this technical solution, the blade and the shading paper are clamped at the same time with a magnet, and the shading paper is pulled out from between the two magnets after a period of dark adaptation. However, when pulling it out, the shading paper may drive the blade to move, causing friction between the blade and the magnet, causing damage to the blade. It is also possible that when the shading plate is pulled out, a gap will appear between the two magnets, resulting in light leakage, which will eventually affect the accuracy of the detection data.

[0004] In view of this, the inventor conducted in-depth research on the above-mentioned defects in the prior art, and thus came up with this case. Utility Model Content

[0005] The utility model aims to provide a dark-adapted leaf clamp for chlorophyll fluorescence detection, which will not damage the leaf when clamping the leaf, and will not cause light leakage to affect the accuracy of the detection data.

[0006] In order to achieve the above technical purpose, the technical solution adopted by the utility model is as follows:

[0007] A dark-adapted leaf clamp for chlorophyll fluorescence detection comprises an upper clamp, a lower clamp and a shading plate, wherein the upper clamp is hinged to the lower clamp; an optical fiber guide hole column is provided on the top of the upper clamp, and a through hole extending downward and penetrating the bottom of the upper clamp is formed on the top of the optical fiber guide hole column; a slot penetrating the cross section of the through hole is also formed on the outer wall of the optical fiber guide hole column, and the shading plate is slidably installed in the slot; a pressing plate is provided on the outer side of the shading plate, and guide columns are provided at both ends of the pressing plate on the side close to the optical fiber guide hole column, and two mounting ears are also formed on the outer wall of the optical fiber guide hole column, and guide holes slidably matched with the guide columns are formed on the mounting ears, and a limiting piece is formed at the end of the guide column.

[0008] Furthermore, the guide column is rotatably mounted on the pressing plate; a limit block is formed on the side wall of the guide column, and a clearance opening for the limit block to pass through is formed on the mounting ear. To prevent the light shielding plate from being pushed out of the slot due to human error by touching the guide column, the guide column can be rotated after the light shielding plate is pressed into the slot, so that the limit block is misaligned with the clearance opening, thereby preventing the light shielding plate from being pushed out of the slot.

[0009] Furthermore, a return spring is sleeved on the guide column, one end of the return spring contacts the mounting ear, and the other end contacts the pressing plate. When the blade adapts to the dark for a period of time, the shading plate needs to be withdrawn from the slot. At this time, the guide column only needs to be rotated to make the limit block correspond to the clearance opening, and the shading plate can be automatically withdrawn from the slot under the action of the return spring.

[0010] Furthermore, a plurality of anti-skid patterns are formed on the side wall of the limit plate, so that the guide column can be rotated more easily through the anti-skid patterns.

[0011] Furthermore, a rubber gasket is formed at the bottom of the upper clamping plate, and the rubber gasket is coaxially arranged with the perforation. The rubber gasket can better protect the blades and prevent them from being pinched.

[0012] After adopting the above structure, the utility model involves a dark-adapted leaf clamp for chlorophyll fluorescence detection. Compared with the prior art, this case slides the shading plate into the slot of the optical fiber guide hole column, and directly clamps the leaf blades through the upper clamping plate and the lower clamping plate when in use. The shading plate does not contact the leaf blades during dark adaptation. Therefore, after the dark adaptation is completed, the shading plate will not cause damage to the leaf blades and will not cause light leakage when it is pulled out. In addition, the limiting plate on the guide column can prevent the shading plate from being completely removed from the optical fiber guide hole column. It can be operated with one hand when in use, which is very convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] The present invention can be further described by the non-limiting embodiments given in the accompanying drawings;

[0014] Figure 1 It is a structural schematic diagram of the utility model;

[0015] Figure 2 for Figure 1 Schematic diagram of the decomposition of

[0016] Figure 3 This is a schematic diagram of the structure of the upper clamping plate in the utility model;

[0017] Figure 4 This is a schematic diagram of the structure of the sunshade in the utility model;

[0018] Figure 5 for Figure 3 Schematic diagram of the local enlarged structure at point A in the middle.

[0019] The symbols of the main components are explained as follows: upper clamping plate 1, optical fiber guide hole column 11, through hole 111, slot 112, mounting ear 113, guide hole 1131, clearance port 1132, rubber gasket 12, lower clamping plate 2, shading plate 3, pressing plate 31, guide column 32, limit plate 321, anti-slip groove 3211, limit block 322, return spring 33. DETAILED DESCRIPTION

[0020] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments. It should be noted that in the drawings or in the specification, similar or identical parts use the same figure numbers, and the implementation methods not shown or described in the drawings are forms known to ordinary technicians in the relevant technical field. In addition, the directional terms mentioned in the embodiments, such as "upper", "lower", "top", "bottom", "left", "right", "front", "back", etc., are only referenced to the directions of the drawings and are not used to limit the scope of protection of the present invention.

[0021] like Figure 1 to Figure 5As shown, the utility model is a dark-adapted leaf clamp for chlorophyll fluorescence detection, comprising an upper clamp, a lower clamp and a shading plate, wherein the upper clamp is hinged with the lower clamp; specifically, a rotating shaft is formed in the middle of the lower clamp, a rotating hole matched with the rotating shaft is formed in the middle of the upper clamp, a torsion spring is arranged between the rotating shaft and the rotating hole to provide a clamping force between the upper clamp and the lower clamp, an optical fiber guide hole column is arranged on the top of the upper clamp, a through hole extending downward and penetrating the bottom of the upper clamp is formed on the top of the optical fiber guide hole column; when detecting, the optical fiber projects a light beam onto the leaf through the through hole. The light shielding plate is on the sheet; a slot penetrating the cross section of the perforation is formed on the outer wall of the optical fiber guide hole column, and the light shielding plate is slidably installed in the slot, so that the light shielding plate can completely cover the perforation, so that the dark adaptation effect is better; the outer side of the light shielding plate has a pressing plate, and the two ends of the pressing plate close to the side of the optical fiber guide hole column are provided with guide columns, and the outer wall of the optical fiber guide hole column is also formed with two mounting ears, and the mounting ears are formed with guide holes that slide with the guide columns, and the ends of the guide columns are formed with limit plates, and the diameter of the limit plates is greater than the diameter of the guide holes; when using the light shielding plate. The limit plate can prevent the light shielding plate from completely separating from the optical fiber guide hole column. Each time it is used, it only needs to press the light shielding plate into the slot, which is convenient to operate. In addition, since the light shielding plate does not come into contact with the blades, the light shielding plate will not cause damage to the blades when moving, and the upper clamp and the lower clamp always keep the blades clamped, and will not cause light leakage.

[0022] Preferably, the guide post is rotatably mounted on the pressing plate; a limit block is formed on the side wall of the guide post, and a clearance opening for the limit block to pass through is formed on the mounting ear. In actual operation, to avoid the light shielding plate being pushed out of the slot by mistake due to the guide post being touched by humans, causing light leakage, after the light shielding plate is pressed into the slot, the guide post can be rotated to misalign the limit block with the clearance opening, thereby preventing the light shielding plate from being pushed out of the slot.

[0023] Preferably, a return spring is also sleeved on the guide column, one end of the return spring contacts the mounting ear, and the other end contacts the pressing plate. When the blade adapts to the dark for a period of time, the shading plate needs to be withdrawn from the slot. At this time, it is only necessary to rotate the guide column to make the limit block correspond to the clearance opening, and the shading plate can be automatically withdrawn from the slot under the action of the return spring. In specific operation, since there are two guide columns, one of the guide columns can be rotated first to align the limit block with the clearance opening, and then the other guide column can be rotated. When the limit block on this guide column is aligned with the clearance opening, the shading plate can be withdrawn from the slot under the action of the two return springs.

[0024] Preferably, a plurality of anti-skid patterns are formed on the side wall of the limiting plate, so that the guide column can be rotated more easily through the anti-skid patterns.

[0025] Preferably, a rubber gasket is formed at the bottom of the upper clamping plate, and the rubber gasket is coaxially arranged with the perforation. The rubber gasket can better protect the blades and prevent them from being pinched. In addition, the rubber gasket is black, which can better block light.

[0026] The method of using the utility model is as follows: first, clamp the blade between the upper clamping plate and the lower clamping plate, then press the shading plate to insert the shading plate into the slot, and then twist the guide column to misalign the limit block with the clearance opening. After a period of dark adaptation, first insert the optical fiber of the chlorophyll fluorescence meter into the perforation at the position of the shading plate, and then rotate the guide column to align the limit block with the clearance opening. Then, under the action of the reset spring, the shading plate is pushed out of the slot, and finally, the optical fiber continues to be inserted into the surface of the blade for measurement.

[0027] The above is a detailed introduction to a dark-adapted leaf clip for chlorophyll fluorescence detection provided by the utility model. The description of the specific embodiment is only used to help understand the method and core idea of ​​the utility model. It should be pointed out that for ordinary technicians in this technical field, without departing from the principle of the utility model, the utility model can also be improved and modified, and these improvements and modifications also fall within the scope of protection of the claims of the utility model.

Claims

1. A dark-adapted leaf clip for chlorophyll fluorescence detection, characterized in that: The optical fiber guide hole column (11) is provided on the top of the upper clamping plate (1), and a through hole (111) extending downward and penetrating the bottom of the upper clamping plate (1) is formed on the top of the optical fiber guide hole column (11); a slot (112) penetrating the cross section of the through hole (111) is also formed on the outer side wall of the optical fiber guide hole column (11); the light shielding plate (3) is slidably mounted on the upper clamping plate (1) and the optical fiber guide hole column (11) is provided with a plurality of optical fiber guide hole columns (11). The slot (112) is provided with a pressing plate (31) on the outside of the shading plate (3), and guide columns (32) are provided at both ends of the pressing plate (31) on a side close to the optical fiber guide hole column (11). Two mounting ears (113) are also formed on the outer wall of the optical fiber guide hole column (11), and guide holes (1131) are formed on the mounting ears (113) for slidingly matching with the guide columns (32), and a limiting piece (321) is formed at the end of the guide column (32).

2. A dark-adapted leaf clip for chlorophyll fluorescence detection according to claim 1, characterized in that: The guide column (32) is rotatably mounted on the pressing plate (31); a limit block (322) is formed on the side wall of the guide column (32), and a clearance opening (1132) for the limit block (322) to pass through is formed on the mounting ear (113).

3. A dark-adapted leaf clip for chlorophyll fluorescence detection according to claim 2, characterized in that: A return spring (33) is also sleeved on the guide column (32), one end of the return spring (33) is in contact with the mounting ear (113), and the other end is in contact with the pressing plate (31).

4. A dark-adapted leaf clip for chlorophyll fluorescence detection according to claim 3, characterized in that: A plurality of anti-slip grooves (3211) are also formed on the side wall of the limiting plate (321).

5. The dark-adapted leaf clip for chlorophyll fluorescence detection according to claim 1, characterized in that: A rubber gasket (12) is also formed at the bottom of the upper clamping plate (1), and the rubber gasket (12) is coaxially arranged with the through hole (111).

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