Composite cell culture device

Abstract

The utility model discloses a combined type cell culture device, and belongs to the field of biology, life science and medical instruments. An incubator (2) comprises a cover (20) and an incubator body (25) with an upper opening, a mechanical sliding type incubator door (23) is clamped between an upper cover (21) and a lower cover (22) of the cover (20), and the cover (20) is pressed above the incubator body (25); a partitioned turntable (27) and a lifting basket (5) are arranged in an inner box body of the culture box body (25), and the lifting basket (5) rotates along with the partitioned turntable (27) and is taken or placed through the box door (23); the culture box (2) is hung below the operation table top of the clean bench, so that the cell culture box and the clean bench are integrally arranged, cells are operated in a clean environment of the clean bench, the practical efficiency of space is improved, and the working efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the fields of biology, life science and medical equipment, and in particular to a composite cell culture device. Background Art

[0002] Cells are the basic structural and functional units of the body. Cells or single-cell organisms are commonly used to study life phenomena and the laws of life. Single cells or simple tissues are also conventional models for studying pathology, pathological mechanisms, drug sensitivity, new drug development, clinical treatment plan formulation and revision, etc. Cell culture is a conventional and commonly used technology to ensure that cells survive for a certain period of time after being separated from the body.

[0003] Since cells have very strict requirements on the living environment for survival, in addition to ensuring normal nutritional support for cells, removal of metabolic waste, stable pH and temperature, it is also necessary to ensure that the cultured cells are not contaminated. The pollution usually comes from the air in the cell culture microenvironment.

[0004] In order to prevent contamination from aerosols (carriers of microorganisms) in the air, a closed air filtration system is needed to ensure a certain temperature, humidity and air pressure of the air, filter out aerosol microorganisms in the air, and form a so-called "cell room". In this cell culture room, six tasks can be carried out: aseptic operation, incubation, preparation, cleaning, disinfection and sterilization, and storage.

[0005] To build a cell room, generally speaking, materials that are pollution-free, radiation-free, smooth, moisture-proof, disinfection-resistant, scrub-resistant, and water-resistant are required in the building, such as the commonly used color steel plates; the floor is made of self-leveling or dust-free PVC flooring, and the eight corners must be rounded. In addition to conventional lighting, an air purification system must also be designed and constructed. In the clean cell room, a clean bench or a biological safety cabinet must be installed to maintain sterile operation and avoid the influence of microorganisms and other harmful factors.

[0006] Incubators, also called incubators, also need to be clean and dust-free, so they should also be set up in areas with little disturbance and not in areas with heavy traffic. Incubation can be done in an incubator or in a temperature-controlled greenhouse, the latter of which is more expensive, and most laboratories work in incubators.

[0007] If it is used for cell culture at the clinical application level, it must be built in strict accordance with GMP standards; if it is used to culture bacteria or viruses, it must be built in strict accordance with the corresponding standards of biosafety levels 2, 3, and 4 (P2, P3, and P4). The high-end configuration that meets these standards has very high construction costs, maintenance costs, and operating costs.

[0008] However, for ordinary scientific research-level cell culture, building and using a constant temperature and humidity air-filtered cell room with a built-in clean bench (biosafety cabinet) and incubator is obviously a waste of construction space, materials, labor, and operating energy (it needs to be turned on 24 hours a day for uninterrupted operation).

[0009] Therefore, in order to meet the needs of scientific research-oriented cell culture, the cell culture system urgently needs to be changed. Summary of the invention

[0010] As shown above, the purpose of the utility model is to overcome the shortcomings of the above-mentioned existing cell culture system and provide a composite cell culture device, which has an ultra-clean workbench and an incubator, does not need to build a special cell room, saves construction and maintenance costs, saves a lot of operating costs, saves a lot of space, and improves the practical efficiency of the space.

[0011] The technical solution of the utility model is as follows:

[0012] The utility model provides a composite cell culture apparatus, which comprises a clean bench and a cell culture box, wherein the clean bench comprises an operating table and a movable glass panel, wherein the movable glass panel is arranged at the upper front of the clean bench, and the clean bench is isolated from the outside world by the movable glass panel to form an operating space;

[0013] The incubator is hung under the operating table of the clean bench through a hanging basket, and comprises a lid and an incubator body with an upper opening, wherein the lid comprises an upper cover and a lower cover, wherein the upper cover is buckled with the lower cover and fixedly connected to form a hollow oblate structure, an upper cover opening is provided on one side of the upper cover, and a lower cover opening corresponding to the upper cover opening is provided on the same side of the lower cover, and a mechanical sliding box door is clamped between the upper cover and the lower cover;

[0014] The box door comprises a sliding guide rail, a movable door, an opening bracket and a pneumatic small sealing ring. The opening bracket is fixedly connected to the lower cover, and the opening of the front half thereof is consistent in size with the opening of the lower cover. The pneumatic small sealing ring is arranged and fixed along the opening of the front half of the opening bracket. The sliding guide rail adopts an H-shaped U-shaped opening silicone strip, which is fixed to the upper surface of the opening bracket. The length of the H-shaped U-shaped opening silicone strip is equivalent to the length of the opening bracket, and the width of the H-shaped U-shaped opening silicone strip is greater than the width of the opening of the front half of the opening bracket. The movable door slides in the U-shaped opening of the sliding guide rail. The upper cover opening, the lower cover opening and the opening of the front half of the opening bracket of the lid form a lid opening with consistent opening shapes and aligned up and down. The lid opening is closed by the movable door, and the lid is pressed on the top of the culture box body.

[0015] The culture box body comprises an inner box body and an outer box body, the inner box body and the outer box body form a hollow two-layer fixed structure, a large pneumatic sealing ring is fixed at the edge of the inner box body by an adhesive, a partition turntable and a basket are arranged in the inner box body of the culture box body, the partition turntable has at least eight convex edges to divide the partition turntable into a plurality of rectangular areas, a basket is placed in each rectangular area, a rotating shaft is arranged at the center of the partition turntable, the partition turntable rotates around the rotating shaft, the basket rotates with the partition turntable and extracts the culture box through the lid opening;

[0016] It also includes a CO2 gas cylinder and a gas pipe. The CO2 gas cylinder is located outside the operating space of the clean bench and is connected to the incubator through the gas pipe to transport CO2 gas to the incubator and the small pneumatic sealing ring and the large pneumatic sealing ring.

[0017] Optionally, the lid opening is rectangular.

[0018] Optionally, the sliding guide rail includes two side edges and two transverse edges, the cross section of the side edge is a U-shaped opening, the openings of the two side edges are opposite to each other, and the two transverse edges are arranged across the two side edges to form an H-shape of an integrated structure.

[0019] Optionally, a plurality of circuit openings are provided on the lower cover, and the circuit openings are located at the rear half of the opening bracket.

[0020] Optionally, the upper surface of the open bracket is provided with a groove surrounding the front half opening thereof, a small pneumatic sealing ring is fixed in the groove, a shaping sheet is provided in the small pneumatic sealing ring, the ventilation opening of the small pneumatic sealing ring is downwardly connected to the pipeline and the solenoid valve, and is connected to the air supply pipe through the line opening.

[0021] Optionally, the basket includes a rectangular three-dimensional frame, a top rod arranged on the top of the rectangular three-dimensional frame, a bottom plate arranged on the bottom of the rectangular three-dimensional frame, and baffles arranged on each window of the rectangular three-dimensional frame.

[0022] Optionally, a direct heating air jacket system is provided in the incubator, and the movable door is electrically heated glass.

[0023] Optionally, a bracket is provided in the center of the partition turntable, and the bracket is in a "X" shape, spanning above the rotating shaft, and its two ends are fixed to the partition turntable.

[0024] Optionally, the partition turntable is provided with a rotation control device, and the rotation control device includes an angle sensor fixed on a bracket.

[0025] Optionally, a human-machine interface is provided in the center of the inner side of the back panel of the clean bench for displaying and setting the gas concentration, working mode, temperature and humidity in the incubator and the rotation angle of the partition turntable.

[0026] Beneficial Effects

[0027] The utility model discloses a composite cell culture apparatus in which a culture box is hung below the operating table of a clean bench, so as to realize the integration of the cell culture box and the clean bench. After operating the cells in the clean environment of the clean bench, the clean bench does not need to be taken out, but can be directly placed in the hanging cell culture box, so that a special cell room does not need to be built, thus saving construction and maintenance costs, saving a lot of operating costs, saving a lot of space, improving the practical efficiency of the space, and improving work efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a schematic diagram of the structure of a composite cell culture device of the utility model;

[0029] Figure 2 for Figure 1 A schematic diagram of the front view structure of

[0030] Figure 3 for Figure 1 Schematic diagram of the structure of the incubator;

[0031] Figure 4 for Figure 3 A schematic diagram of the exploded structure of the middle cover;

[0032] Figure 5 for Figure 4 The schematic diagram of the structure without the upper cover;

[0033] Figure 6 and Figure 7 for Figure 5 A schematic diagram of a small pneumatic sealing ring and its longitudinal section;

[0034] Figure 8 for Figure 3 A schematic diagram of the structure of the incubator without a basket;

[0035] Fig. 9 for Figure 8 Schematic diagram of the structure of the incubator;

[0036] Fig.10 for Figure 2 A schematic diagram of the structure of the basket placed in the incubator;

[0037] Fig.11 for Fig.10 A schematic diagram of the structure of the middle basket;

[0038] Fig.12 for Fig.10 Schematic diagram of the use status of the middle basket;

[0039] Among them: clean workbench 1, operating table 11, control button 12, movable glass panel 13, clean device 15, human-machine interface 17, hanging basket 18;

[0040] Incubator 2, lid 20, upper cover 21, upper cover opening 211, lower cover 22, lower cover opening 221, line opening 225, sliding guide rail 231, movable door 232, opening bracket 235, pneumatic small sealing ring 237, shaping sheet 238;

[0041] Culture box 25, large pneumatic sealing ring 26, through hole 251;

[0042] Partition turntable 27, convex ridge 271, rectangular area 272, sensor 274, bracket 275, rotating shaft 276;

[0043] CO2 gas cylinder 3, gas pipe 31, pressure reducing valve 32;

[0044] Basket 5, top rod 51, bottom plate 53, baffle 55, and culture plate 57. DETAILED DESCRIPTION

[0045] In order to make the purpose, technical solution and advantages of the present invention more clear, the implementation mode of the present invention will be further described in detail below with reference to the accompanying drawings.

[0046] The utility model discloses a composite cell culture device. Figure 1 Schematic diagram of the structure of the composite cell culture device. Figure 1 As shown, the composite cell culture apparatus comprises: a clean bench 1, an incubator 2 and a CO2 gas cylinder 3. The CO2 gas cylinder 3 is located outside the operating space of the clean bench 1 and is connected to the incubator 2 via a gas pipe 31. A pressure reducing valve 32 is also provided on the gas pipe 31 of the CO2 gas cylinder 3.

[0047] A movable glass panel 13 is provided above the operating table 11 of the clean bench 1, and the movable glass panel 13 and the clean bench 1 form a clean operating space isolated from the outside world for cell culture and observation. The clean bench 1 has an ultra-clean device 15 disposed on its top.

[0048] The working principle of the ultra-clean device 15 of the ultra-clean bench is as follows: in a specific space, the indoor air is initially filtered through a coarse filter, pressed into a static pressure box by a centrifugal fan, and then finely filtered through a high-efficiency air filter. The clean airflow sent from the high-efficiency air filter passes through the sterile area at a certain uniform cross-sectional wind speed, which can remove the original air in the working area and take away dust particles and biological particles, thereby forming a dust-free and sterile high-cleanliness working environment. The ultra-clean bench is divided into a vertical flow ultra-clean bench (Vertical Flow Clean Bench) and a horizontal flow ultra-clean bench (Horizontal Flow Clean Bench) according to the direction of the airflow; the purification level of the ultra-clean bench can reach Class 100 through air filtration.

[0049] In each embodiment of the present utility model, the clean bench 1 can be a vertical flow clean bench or a horizontal flow clean bench; it can be a single-side operation clean bench or a double-side operation clean bench; it can be a single-person workbench or a double-person workbench.

[0050] The following two points should be noted when using a clean bench: (1) The clean bench should be installed in an isolated sterile room or a clean and dust-free room, where there is less traffic or airflow, to prevent excessive dust from blocking the filter, reducing the purification effect and shortening its service life. (2) For a newly installed or long-unused clean bench, the workbench and the surrounding environment must be cleaned with a vacuum cleaner or fiber-free tools before work, and then sterilized using drug sterilization or ultraviolet sterilization.

[0051] In this embodiment, preferably, the movable glass panel 13 above the operating table 11 of the clean bench 1 can be manually raised or lowered. The operating space above the operating table 11 of the clean bench 1 is opened or closed by raising or lowering the movable glass panel 13. When the operator needs to operate, the movable glass panel 13 is raised, so that the operator can conveniently operate on the operating table 11 of the clean bench 1; when the operator finishes the operation, the movable glass panel 13 is lowered in time, so that the operating space above the operating table 11 of the clean bench 1 forms a clean space, which can better maintain a dust-free and sterile high-cleanliness working environment in the operating space.

[0052] Furthermore, a control button 12 is provided on the outer side of the clean bench 1 for controlling the lighting, air filtration, ultraviolet lamp and heating system of the clean bench 1. In addition, a fingerprint recognition system may be provided on the outer side of the clean bench 1 for controlling the operator through fingerprints to realize identity recognition.

[0053] In a preferred embodiment of the present invention, the operating table 11 of the clean bench 1 is 1.2-1.5 meters long and 60-80 centimeters wide. Of course, this is only a preferred embodiment. In other embodiments of the present invention, the operating table 11 of the clean bench 1 can also be designed in other sizes, and is not limited thereto.

[0054] The incubator 2 is hung on one side of the operating table 11 of the clean bench 1 through the hanging basket 18. Figure 1 As shown, the shape of the hanging basket 18 is not limited, and the principle is to quickly install, disassemble and use conveniently. The incubator 2 is oblate so as not to hinder the leg space of the operator when operating the experiment.

[0055] The incubator 2 includes a cover 20 and an upper opening incubator body 25, the main components of which are made of high-quality stainless steel and have a certain weight. Figure 3 As shown, it is a schematic diagram of the overall structure of the incubator 2.

[0056] The cover 20 includes an upper cover 21 and a lower cover 22. The upper cover 21 is buckled and fixedly connected with the lower cover 22 to form a hollow oblate structure. Figure 4 As shown. An upper cover opening 211 is provided on one side of the upper cover 21, and is in a rectangular shape. A lower cover opening 221 corresponding to the upper cover opening 211 is provided on the same side of the lower cover 22, and is also in a rectangular shape. A mechanical sliding door 23 is clamped between the upper cover 21 and the lower cover 22. The door 23 includes a sliding guide rail 231, a movable door 232, an opening bracket 235, and a pneumatic small sealing ring 237, as shown in FIG. Figure 5 Specifically, the opening bracket 235 is made of acrylic with a thickness of about 1 cm, and is fixedly connected to the lower cover 22. The opening of the front half is the same size as the lower cover opening 221 and is rectangular. The rear half of the opening bracket 235 is an extension of the two long sides of the rectangle, and a circuit opening 225 is provided on the lower cover 22 therein.

[0057] A groove (not shown) is provided on the upper surface of the opening bracket 235 and surrounds the front half of the opening thereof. A pneumatic small sealing ring 237 is fixed in the groove, and the pneumatic small sealing ring 237 is rectangular in the groove. The provision of the groove can enhance the firmness of the installation of the pneumatic small sealing ring 237, and it is not easy to be carried away by the moving door 232 during the opening and closing process of the moving door 232. The ventilation opening of the pneumatic small sealing ring 237 is connected to the pipeline and the electromagnetic valve downward, and is connected to the gas supply pipe 31 through the line opening 225.

[0058] In order to prevent the pneumatic small sealing ring 237 from shrinking and rising inward (in the x direction) when the vacuum is drawn in the rectangular groove, a shaping sheet 238 is inserted into the pneumatic small sealing ring 237 to shrink longitudinally (in the y direction) when the vacuum is drawn. The shaping sheet 238 is a flat silicone sheet, which has the same shape as the groove and has an opening for passing through the pneumatic small sealing ring 237. Its width in the x direction is smaller than the inner diameter of the pneumatic small sealing ring 237 when it is fully inflated. Figure 6 and Figure 7 As shown, Figure 5 The middle pneumatic small sealing ring 237 and its longitudinal cross-sectional diagram.

[0059] The sliding guide 231 adopts an H-shaped U-shaped opening silicone strip. Specifically, the sliding guide 231 includes two side edges and two transverse edges. The cross section of the side edge is a U-shaped opening. The openings of the two side edges are opposite to each other. The two transverse edges are arranged across the two sides of the two side edges, forming an H-shaped structure of an integrated structure. The sliding guide 231 is fixed to the upper surface of the opening bracket 235. Its length is equivalent to the length of the opening bracket 235, and its width is greater than the width of the front half opening of the opening bracket 235. The movable door 232 slides in the U-shaped opening of the sliding guide 231. Generally, the movable door 232 adopts electrically heated glass to prevent the formation of water mist. The upper cover opening 211, the lower cover opening 221 of the cover 20 and the front half opening of the opening bracket 235 form a cover opening with a consistent opening shape and aligned up and down.

[0060] When in use, the movable door 232 moves in the lid opening, and when it slides to the fully closed position, the solenoid valve is controlled to inflate the pneumatic small sealing ring 237, and the pneumatic small sealing ring 237 expands to slightly lift the movable door 232, thereby increasing the upward pressure of the movable door 232 and the silicone strip of the sliding guide rail 231, and completing the seal. When the movable door 232 is to be opened, the solenoid valve is first controlled to evacuate the pneumatic small sealing ring 237, so that the pneumatic small sealing ring 237 shrinks, and the upward pressure of the movable door 232 and the silicone strip of the sliding guide rail 231 is reduced, and then the movable door 232 is slid to the fully open position.

[0061] The culture box 25 includes an inner box and an outer box, which form a hollow two-layer fixed structure, and are filled with heat-insulating materials to maintain a constant temperature. The outer box is provided with an opening (not shown) for the gas pipe 31 and the circuit of the electronic components to enter and exit.

[0062] A large pneumatic sealing ring 26 is fixed to the edge of the inner box body by adhesive. The large pneumatic sealing ring 26 is circular. The ventilation opening 233 of the large pneumatic sealing ring 26 faces downward, and its air supply pipe passes through the through hole 251 on the edge of the culture box body 25 and is connected to the air supply pipe 31, and is equipped with a solenoid valve. When in use, the large pneumatic sealing ring 26 is inflated through the solenoid valve, and the lid 20 uses its own weight to press and connect with the culture box body 25 through the large pneumatic sealing ring 26. When disassembling, just lift the lid, which is convenient for cleaning the inside of the culture box body 25. Figure 8 shown.

[0063] A partition turntable 27 is provided in the inner box of the culture box 25. The partition turntable 27 has at least eight ridges 271 that divide the partition turntable 27 into a plurality of rectangular areas 272. Four, six or eight areas can be formed as needed, and a basket 5 is placed in each area. Fig.10 shown. Fig. 9 In the figure, 12 convex ribs 271 are shown, and each group of two convex ribs 271 divides the partition turntable 27 into 6 rectangular areas 272. A rotating shaft 276 is provided at the center of the partition turntable 27, and the partition turntable 27 rotates around the rotating shaft 276. The basket 5 can rotate with the partition turntable 27.

[0064] A bracket 275 for fixing the angle sensor is arranged in the center of the partition turntable 27. The bracket 275 is in a "+" shape and spans over the rotating shaft 276. Both ends of the bracket 275 are fixed to the partition turntable 27. Fig. 9 The basket 5 includes a rectangular three-dimensional frame, a top rod 51 arranged at the top of the rectangular three-dimensional frame, a bottom plate 53 arranged at the bottom of the rectangular three-dimensional frame, and baffles 55 arranged at each window of the rectangular three-dimensional frame. A plurality of vessels for culturing cells can be placed on the bottom plate 53, such as Fig.11 The vessel for culturing cells may be a culture dish, a culture plate or a culture flask.

[0065] Generally, each basket 5 is used to place different single culture vessels, which is convenient for targeted management. The baffle 55 may not be provided on one side of the short side of the basket 5, so as to facilitate the insertion and placement of long culture plates or slides, such as Fig.12 As shown, a culture plate 57 is placed on a bottom plate 53. The basket 5 is made of stainless steel. The baffle 55 can be used to mark a mark. The shape of the basket 5 is consistent with the shape of the lid opening, and is slightly smaller, so that the basket 5 can be easily taken out through the lid opening.

[0066] Furthermore, a rotation control device is provided in the partition turntable 27, and the rotation control device includes an angle sensor fixed on the bracket 275, which is used to control the movement of the partition turntable 27 and the extraction of the designated basket 5. Fig.10When the movable door 232 is opened, the angle of rotation of the partition turntable 27 is displayed on the screen of the human-machine interface 17 through the angle sensor, as shown in FIG. Figure 2 shown.

[0067] A gas concentration sensor, such as an O2 concentration sensor or a CO2 concentration sensor, is arranged in the hollow structure of the lid 20 of the incubator 2, and the gas concentration is controlled by a single chip microcomputer to ensure the constant gas concentration in the incubator. The CO2 incubator can provide a certain amount of CO2 (usually 5%) required for cell culture.

[0068] When in use, open the movable door 232, grab the top rod 51 through the lid opening by hand or an instrument to pull up the basket 5, place the basket 5 in the operation area, observe through the observation window, or take out the culture vessel or slide from the basket 5 for observation, so as to realize the integration of the cell culture box and the clean bench. The operator can extract the sample in the basket 5 in the incubator at any time at the clean bench, without repeatedly moving the culture back and forth between the clean bench and the incubator 2, and without frequently opening and closing the movable door 232 of the incubator, which effectively reduces the potential damage to the sample caused by environmental factors such as CO2 concentration, temperature, osmotic pressure, light, humidity, and physical shaking.

[0069] The components of the incubator 2 can be arranged on the body of the clean bench 1, and are completely integrated with the clean bench 1 in design and construction. The incubator 2 is no longer an independent unit, but a composite integrated incubator is formed. The incubator 2 is provided with a direct heating gas jacket system, so that the in vitro cultured cells can survive at a constant temperature like the in vivo cells. In most cases, the optimum temperature is 37°C, and the temperature difference should generally not exceed ±0.5°C.

[0070] In this embodiment, preferably, a human-machine interface 17 is provided on the outer surface of the clean bench, which is used to display and set control parameters such as gas concentration, working mode, temperature and humidity in the incubator 2; further, a barcode or QR code recognition device is also provided on the outer surface of the incubator 2, which is used to record and identify the experimental process of culturing cells, scan and record the entry and exit of culture vessels, which becomes a part of the experimental record, and can realize wireless communication with wireless terminals such as mobile phones.

[0071] Furthermore, the incubator 2 may also be provided with a wireless communication device for sending various control parameters of the incubator to the wireless terminal of the operator.

[0072] The incubator built into the clean bench has all the functions of an ordinary cell culture box, such as constant temperature and humidity, 5% CO2 concentration, clean and dust-free. The incubator is equipped with a human-machine interface to facilitate observation and setting of various parameters in the incubator, making it more convenient to use. The control parameters of the incubator can communicate wirelessly with wireless terminals such as mobile phones, and the parameters of each culture vessel (QR code or barcode recognition) (such as the time to change the culture medium) can be sent to the operator's mobile phone in a timely manner, and a prompt will be given at that time; or when the parameters change, the mobile phone will alarm.

[0073] It should be noted that fingerprint recognition systems, automatic sensing systems, human-machine interfaces, barcode or QR code recognition devices, wireless communication devices, gas concentration sensors, and single-chip microcomputer controls are all mature technologies in the existing art and will not be introduced in detail here.

[0074] In a preferred embodiment of the present invention, the incubator 2 can also be a bioreactor, with a diameter of 50-60 cm and a height of 30-40 cm, and multiple cell culture dishes, culture plates or culture bottles can be placed inside, and the shape is compact and space-saving. Of course, this is only a preferred embodiment. In other certain embodiments of the present invention, the incubator 2 can also be designed for other dimensions, but is not limited thereto and can be changed according to the actual requirements of the user. The inner wall of the clean bench 1 and the inner wall of the incubator 2 can be sprayed with antibacterial nanomaterials, such as nanosilver. Nanosilver can ensure that bacteria will not be attached.

[0075] The composite cell culture apparatus can be placed in an ordinary laboratory and operated independently, completing all the work of a traditional cell room + clean bench + incubator, saving the construction and maintenance of the cell room, and is suitable for cell culture at the level of ordinary scientific research.

[0076] In an ordinary laboratory, after the operation on the clean bench 1 is completed, the cell culture dish is transferred to the incubator 2 built into the clean bench. If the operating procedures are strictly followed, cell contamination generally will not occur, and the cell culture requirements of the ordinary scientific research level can be met.

[0077] In summary, the composite cell culture device directly hangs the culture box 2 inside the clean bench 1, and the components are integrated on the clean bench body to realize the integration of the cell culture box and the clean bench. After operating the cells in the clean environment of the clean bench, there is no need to take out the clean bench, and it can be directly placed in the embedded cell culture box. There is no need to build a special cell room, which saves construction costs, maintenance costs, and operating costs, saves a lot of space, and improves the practical efficiency of the space. The composite cell culture device can be directly placed in an ordinary laboratory to operate normally, without the need to build a special "cell room", saving the construction cost of the cell room. The construction of traditional cell rooms is expensive, and the system construction includes: HVAC, wind control, automatic control, wall construction, ground construction, weak current and strong current, etc. The construction cost is about 1.8 million / 200 square meters. It saves the 24-hour operation cost (electricity fee) of the cell room, saves the maintenance cost of the cell room (replacement and renewal of the filter membrane of the ventilation system, and aging of mechanical and electronic equipment), etc. At the same time, it saves a lot of space (no need to build a separate house), and improves the efficiency of space use. Of course, the composite cell culture device can be placed in a traditional cell room or in a GMP laboratory, and is suitable for cell culture at the clinical application level.

[0078] It should be noted that, although the present invention has been illustrated and described with reference to certain preferred embodiments of the present invention, those skilled in the art should understand that various changes may be made in form and details without departing from the spirit and scope of the present invention.

Claims

1. A composite cell culture apparatus, comprising a clean bench (1) and a cell culture box (2), wherein the clean bench (1) comprises an operating table (11) and a movable glass panel (13), wherein the movable glass panel (13) is arranged at the upper front of the clean bench (1), and the clean bench (1) is isolated from the outside world by the movable glass panel (13) to form an operating space; It is characterized in that The incubator (2) is hung below the operating table (11) of the clean bench (1) through a hanging basket (18), and comprises a cover (20) and an incubator body (25) with an upper opening, wherein the cover (20) comprises an upper cover (21) and a lower cover (22), wherein the upper cover (21) is buckled and fixedly connected to the lower cover (22) to form a hollow oblate structure, an upper cover opening (211) is provided on one side of the upper cover (21), and a lower cover opening (221) corresponding to the upper cover opening (211) is provided on the same side of the lower cover (22), and a mechanical sliding box door (23) is clamped between the upper cover (21) and the lower cover (22); The box door (23) comprises a sliding guide rail (231), a movable door (232), an opening bracket (235) and a pneumatic small sealing ring (237); the opening bracket (235) is fixedly connected to the lower cover (22); the front half opening of the opening bracket (235) is the same size as the lower cover opening (221); the pneumatic small sealing ring (237) is arranged and fixed along the front half opening of the opening bracket (235); The sliding guide rail (231) is an H-shaped U-shaped opening silicone strip, fixed to the upper surface of the opening bracket (235), with a length equivalent to the length of the opening bracket (235) and a width greater than the width of the front half opening of the opening bracket (235), and the movable door (232) slides in the U-shaped opening of the sliding guide rail (231); The upper cover opening (211), the lower cover opening (221) and the front half opening of the opening bracket (235) of the cover (20) form a cover opening with the same opening shape and aligned up and down, and the cover opening is closed by the movable door (232), and the cover (20) is pressed on the top of the culture box (25); The culture box body (25) comprises an inner box body and an outer box body, the inner box body and the outer box body form a hollow two-layer fixed structure, a large pneumatic sealing ring (26) is fixed at the edge of the inner box body by an adhesive, a partition turntable (27) and a basket (5) are arranged in the inner box body of the culture box body (25), the partition turntable (27) has at least eight convex edges (271) to divide the partition turntable (27) into a plurality of rectangular areas (272), a basket (5) is placed in each rectangular area (272), a rotating shaft (276) is arranged at the center of the partition turntable (27), the partition turntable (27) rotates around the rotating shaft (276), the basket (5) rotates with the partition turntable (27) and extracts the culture box (2) through the lid opening; It also includes a CO2 gas cylinder (3) and a gas pipe (31). The CO2 gas cylinder (3) is located outside the operating space of the clean bench (1) and is connected to the incubator (2) through the gas pipe (31) to transport CO2 gas to the incubator (2) and the small pneumatic sealing ring (237) and the large pneumatic sealing ring (26).

2. The composite cell culture apparatus according to claim 1, characterized in that: The lid opening is rectangular.

3. The composite cell culture apparatus according to claim 1, characterized in that: The sliding guide rail (231) comprises two side edges and two transverse edges, the cross section of the side edges is a U-shaped opening, the openings of the two side edges are opposite to each other, and the two transverse edges are arranged across the two sides of the two side edges to form an H-shaped integral structure.

4. The composite cell culture apparatus according to claim 1, characterized in that: A plurality of circuit openings (225) are provided on the lower cover (22), and the circuit openings (225) are located at the rear half of the opening bracket (235).

5. The composite cell culture apparatus according to claim 4, characterized in that: The upper surface of the opening bracket (235) is provided with a groove surrounding the front half opening thereof, a small pneumatic sealing ring (237) is fixed in the groove, a shaping sheet (238) is provided in the small pneumatic sealing ring (237), the ventilation opening of the small pneumatic sealing ring (237) is connected to the pipeline and the solenoid valve downward, and is connected to the air supply pipe (31) through the line opening (225).

6. The composite cell culture apparatus according to claim 1, characterized in that: The basket (5) comprises a rectangular three-dimensional frame, a top rod (51) arranged at the top of the rectangular three-dimensional frame, a bottom plate (53) arranged at the bottom of the rectangular three-dimensional frame, and baffles (55) arranged at each window of the rectangular three-dimensional frame.

7. The composite cell culture apparatus according to claim 1, characterized in that: The incubator (2) is provided with a direct heating air jacket system, and the movable door (232) is electrically heated glass.

8. The composite cell culture apparatus according to claim 1, characterized in that: A bracket (275) is arranged in the center of the partition turntable (27). The bracket (275) is in a "+" shape and spans above the rotating shaft (276). Both ends of the bracket (275) are fixed to the partition turntable (27).

9. The composite cell culture apparatus according to claim 8, characterized in that: The partition turntable (27) is provided with a rotation control device, and the rotation control device comprises an angle sensor fixed on a bracket (275).

10. The composite cell culture apparatus according to claim 9, characterized in that: A human-machine interface (17) is provided at the center of the inner side of the back panel of the clean bench (1) for displaying and setting the gas concentration, working mode, temperature and humidity in the incubator (2) and the rotation angle of the partition turntable (27).

Images