96-well plate filtering auxiliary device

Abstract

The utility model discloses a 96-pore plate filtering auxiliary device, which belongs to the field of medical equipment and comprises a pore plate body and a row gun arranged on the pore plate body. A positioning assembly for positioning the position of the multi-row gun is arranged on the pore plate body; the positioning assembly comprises a positioning frame plate which is arranged above the pore plate body and arranged outside the row gun in a sleeving mode, a bottom plate is arranged at the bottom of the pore plate body, supporting frames connected with the positioning frame plate are symmetrically arranged at the top end of the bottom plate, and moving mechanisms used for moving the positioning frame plate are arranged on the supporting frames. According to the auxiliary device, by arranging the positioning assembly and the moving mechanism, it can be ensured that the sample adding position of each hole is accurate through the positioning assembly, errors caused by manual operation are reduced, direct contact of human hands can be reduced by using the positioning assembly and the driving mechanism, the risk of cross contamination is reduced, and the experiment accuracy is ensured; the physical and mental burdens of an operator can be greatly reduced by adopting a mechanical positioning and driving mode.

Description

Technical Field

[0001] The utility model relates to the technical field of medical equipment, in particular to a 96-well plate filtering auxiliary device. Background Art

[0002] 96-well plate is a plate made of imported optically transparent pure polystyrene with a large number of culture wells, which is widely used for cell culture. During the cell culture process, the 96-well plate often needs to be replaced. At this time, cell transfer operation is required, which is usually sucked and transferred with a gun, and then injected into a new 96-well plate. Because the distance between adjacent culture wells is small, adjacent cell culture fluids are prone to mutual penetration during the transfer process, which will affect the culture results.

[0003] After investigation, the Chinese utility model patent with publication number CN210683811U discloses a 96-well plate filtration auxiliary device. A box body for placing the well plate, a cover body located above the box body, and an adsorption membrane arranged between the cover body and the well plate, the opening direction of the box body is upward, and a side of the box body is provided with a mounting port for inserting the well plate in communication with the opening, the box body and the cover body are provided with mutually matching locking structures, the cover body is provided with a pipetting hole corresponding to each culture well of the well plate, and the pipetting hole is provided with a pressing ring fixed on the cover body on the outer side of one end of the well plate. The utility model has a simple structure, low manufacturing and use costs, and is easy to operate. It can effectively avoid the mutual interference of cell culture fluids between adjacent wells, and can improve the efficiency and accuracy of the pipetting of the gun, saving operation time. However, the device is not conducive to pipetting a large number of 96-well plates. After the pipetting of one of the 96-well plates is completed, the cover body and the pipetting hole in contact with the film surface need to be cleaned to avoid the risk of cross contamination, resulting in low pipetting efficiency.

[0004] Therefore, the utility model provides a 96-well plate filtering auxiliary device to solve the above problems. Utility Model Content

[0005] 1. Technical issues to be resolved

[0006] The utility model provides a 96-well plate filtering auxiliary device, which aims to solve the problem that the existing auxiliary device proposed in the background technology is not conducive to pipetting a large number of 96-well plates. After the pipetting of one of the 96-well plates is completed, the cover body and the pipetting hole in contact with the film surface need to be cleaned to avoid the risk of cross contamination, resulting in low pipetting efficiency and other problems.

[0007] (II) Technical solution

[0008] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a 96-well plate filtration auxiliary device, comprising a well plate body and a discharge gun arranged on the well plate body;

[0009] The difference from the existing auxiliary device is that: the orifice plate body is provided with a positioning component for positioning the row gun; the positioning component includes a positioning frame plate arranged above the orifice plate body and sleeved on the outside of the row gun, the bottom of the orifice plate body is provided with a bottom plate, the top of the bottom plate is symmetrically provided with a support frame connected to the positioning frame plate, and the support frame is provided with a moving mechanism for moving the positioning frame plate; the auxiliary device can ensure the accuracy of the sample loading position of each hole through the positioning component and the moving mechanism, thereby reducing the error of human operation, and using the positioning component and the driving mechanism can reduce direct contact with human hands, reduce the risk of cross contamination, and ensure the accuracy of the experiment; the use of mechanical positioning and driving methods can greatly reduce the physical and mental burden of the operator;

[0010] The moving mechanism comprises a guide rail fixedly connected to the support frame, and a sliding sleeve at the top of the guide rail is provided with a sliding block connected to a positioning frame plate.

[0011] Preferably, a positioning groove for the orifice plate body to be inserted is provided at the top of the bottom plate; the orifice plate body can be positioned by setting the positioning groove, so that the discharge gun is conveniently aligned with the filter holes on the orifice plate body.

[0012] Preferably, both ends of the bottom plate are provided with opening grooves connected with the positioning grooves; the orifice plate body can be conveniently taken out from the interior of the positioning grooves through the opening grooves.

[0013] Preferably, the top end of the guide rail is symmetrically fixedly connected to a limit plate, and the slider is located between the two limit plates.

[0014] Preferably, the four corners of the bottom end of the base plate are fixedly connected with support legs, and the bottom ends of the support legs are fixedly connected with anti-skid pads; the anti-skid pads are made of epoxy resin paint, and the friction between the device and the workbench can be improved by the anti-skid pads.

[0015] Preferably, the auxiliary device also includes an adjusting mechanism arranged on the slider for adjusting the height of the positioning frame plate, the adjusting mechanism includes a support plate fixed to the slider by bolts and connecting plates fixed on both sides of the positioning frame plate, one of the support plates is threadedly inserted with an adjusting screw rotatably connected to one of the connecting plates, the top of the adjusting screw is fixedly connected to a rotary plate, and the other support plate is slidably inserted with a guide slide bar fixedly connected to the other connecting plate, and the top of the guide slide bar is fixedly connected to a baffle; the auxiliary device can adjust the height of the positioning frame plate through the adjusting mechanism, and then can adjust the height of the discharge gun on the orifice plate body, so that the needle tube on the discharge gun is appropriately positioned away from the filter hole on the orifice plate body, thereby reducing the occurrence of splashing of dripping medicine.

[0016] (III) Beneficial effects

[0017] The utility model improves the auxiliary device and utilizes the design of the positioning component and the moving mechanism. The positioning component can ensure that the sample addition position of each hole is accurate, thereby reducing the error of human operation. The use of the positioning component and the driving mechanism can reduce direct contact with human hands, reduce the risk of cross contamination, and ensure the accuracy of the experiment. The use of mechanical positioning and driving methods can greatly reduce the physical and mental burden of the operator and improve the efficiency of pipetting.

[0018] The auxiliary device is equipped with an adjustment mechanism, which can adjust the height of the positioning frame plate, and then adjust the height of the discharge gun on the orifice plate body, so that the needle tube on the discharge gun is appropriately located away from the filter hole on the orifice plate body, thereby reducing the splashing of dripping medicine. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall structure of a 96-well plate filtration auxiliary device;

[0020] Figure 2 for Figure 1 A bottom view of the overall structure of a 96-well plate filtration auxiliary device;

[0021] Figure 3 is a schematic diagram of the bottom plate structure;

[0022] Figure 4 It is a schematic diagram of the structure of the moving mechanism and the adjusting mechanism.

[0023] In the figure:

[0024] 1. Orifice plate body; 2. Discharge gun; 3. Positioning frame plate; 4. Moving mechanism; 41. Guide rail; 42. Sliding block; 43. Limiting plate; 44. Support frame; 5. Bottom plate; 51. Positioning slot; 52. Opening slot; 6. Support leg; 61. Anti-slip pad; 7. Adjusting mechanism; 71. Support plate; 72. Connecting plate; 73. Adjusting screw; 731. Rotating plate; 74. Guide slide rod; 741. Baffle. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0026] Example 1

[0027] The utility model provides a 96-well plate filtering auxiliary device, such as Figure 1-4 As shown, the auxiliary device includes a perforated plate body 1 and a discharge gun 2 arranged on the perforated plate body 1;

[0028] The well plate body 1 is provided with a positioning component for positioning the row gun 2; the positioning component includes a positioning frame plate 3 arranged above the well plate body 1 and sleeved on the outside of the row gun 2, a bottom plate 5 is arranged at the bottom of the well plate body 1, a support frame 44 connected to the positioning frame plate 3 is symmetrically arranged at the top of the bottom plate 5, and a moving mechanism 4 for moving the positioning frame plate 3 is arranged on the support frame 44; the positioning component can ensure that the sample loading position of each hole is accurate, reduce the error of human operation, and improve the consistency and repeatability of experimental results; the semi-automatic moving mechanism 4 can significantly speed up the sample loading speed, especially when processing a large number of samples, compared with manual sample loading, it can save a lot of time, and is suitable for high-throughput screening and large-scale research projects; the use of positioning components and driving mechanisms can reduce direct contact with human hands, reduce the risk of cross contamination, and ensure the accuracy of the experiment; the use of mechanical positioning and driving methods can greatly reduce the physical and mental burden of the operator;

[0029] The moving mechanism 4 comprises a guide rail 41 fixedly connected to a support frame 44 , and a sliding block 42 connected to the positioning frame plate 3 is slidably sleeved on the top of the guide rail 41 .

[0030] Furthermore, a positioning groove 51 for the orifice plate body 1 to be inserted is provided at the top of the bottom plate 5 ; the orifice plate body 1 can be positioned by setting the positioning groove 51 , so that the discharge gun 2 is conveniently aligned with the filter holes on the orifice plate body 1 .

[0031] Furthermore, both ends of the bottom plate 5 are provided with opening grooves 52 communicating with the positioning groove 51 ; the orifice plate body 1 can be conveniently taken out from the interior of the positioning groove 51 through the opening grooves 52 .

[0032] The top end of the guide rail 41 is symmetrically fixedly connected with the limiting plates 43 , and the slider 42 is located between the two limiting plates 43 ; the limiting plates 43 can prevent the slider 42 from moving out of the guide rail 41 .

[0033] When in use, first insert the discharge gun 2 into the positioning frame plate 3, then align the needle tube on the discharge gun 2 with the filter hole on the orifice plate body 1 through the cooperation of the slider 42 and the guide rail 41, and then drive the discharge gun 2 to move through the cooperation of the slider 42 and the guide rail 41, so that the liquid on the discharge gun 2 accurately drips into the filter hole on the orifice plate body 1.

[0034] The four corners of the bottom end of the base plate 5 are fixedly connected with support legs 6, and the bottom ends of the support legs 6 are fixedly connected with anti-skid pads 61; the anti-skid pads 61 are made of epoxy resin paint, and the friction between the device and the workbench can be improved by the anti-skid pads 61.

[0035] Example 2

[0036] See attached Figure 1 and attached Figure 4 As shown, the auxiliary device also includes an adjusting mechanism 7 arranged on the slider 42 for adjusting the height of the positioning frame plate 3. The adjusting mechanism 7 includes a support plate 71 fixed to the slider 42 by bolts and connecting plates 72 fixed on both sides of the positioning frame plate 3. An adjusting screw 73 rotatably connected to one of the connecting plates 72 is threadedly inserted on one of the support plates 71, and a rotary plate 731 is fixedly connected to the top of the adjusting screw 73. A guide slide bar 74 fixedly connected to the other connecting plate 72 is slidably inserted on the other support plate 71, and a baffle 741 is fixedly connected to the top of the guide slide bar 74. The height of the positioning frame plate 3 can be adjusted by the adjusting mechanism 7, and then the height of the discharge gun 2 on the orifice plate body 1 can be adjusted, so that the needle tube on the discharge gun 2 is appropriately located away from the filter hole on the orifice plate body 1, thereby reducing the occurrence of splashing of dripping medicine.

[0037] During use, when it is necessary to adjust the distance between the row gun 2 and the orifice plate body 1, first rotate the adjusting screw 73, and the adjusting screw 73 drives the bottom connecting plate 72 to move, and at the same time the guide slide bar 74 slides along the other support plate 71; when the adjusting screw 73 is rotated clockwise, the row gun 2 moves down; when the adjusting screw 73 is rotated counterclockwise, the row gun 2 moves up.

[0038] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A 96-well plate filtration auxiliary device, comprising a well plate body (1) and a discharge gun (2) arranged on the well plate body (1); Features: The orifice plate body (1) is provided with a positioning component for positioning the volley gun (2); The positioning assembly comprises a positioning frame plate (3) arranged above the orifice plate body (1) and sleeved on the outside of the row gun (2); a bottom plate (5) is arranged at the bottom of the orifice plate body (1); a support frame (44) connected to the positioning frame plate (3) is symmetrically arranged at the top of the bottom plate (5); and a moving mechanism (4) for moving the positioning frame plate (3) is arranged on the support frame (44); The moving mechanism (4) comprises a guide rail (41) fixedly connected to a support frame (44), and a sliding sleeve at the top of the guide rail (41) is provided with a sliding block (42) connected to the positioning frame plate (3).

2. The 96-well plate filtration auxiliary device according to claim 1, characterized in that: The top end of the bottom plate (5) is provided with a positioning groove (51) for the orifice plate body (1) to be inserted into.

3. The 96-well plate filtration auxiliary device according to claim 2, characterized in that: Open grooves (52) communicating with the positioning grooves (51) are formed at both ends of the bottom plate (5).

4. The 96-well plate filtration auxiliary device according to claim 1, characterized in that: The top end of the guide rail (41) is symmetrically fixedly connected to the limit plates (43), and the slide block (42) is located between the two limit plates (43).

5. The 96-well plate filtration auxiliary device according to claim 1, characterized in that: The four corners of the bottom end of the base plate (5) are fixedly connected to support legs (6), and the bottom ends of the support legs (6) are fixedly connected to anti-slip pads (61).

6. The 96-well plate filtration auxiliary device according to claim 1, characterized in that: The auxiliary device also includes an adjustment mechanism (7) arranged on the slider (42) for adjusting the height of the positioning frame plate (3), the adjustment mechanism (7) including a support plate (71) fixed to the slider (42) by bolts and connecting plates (72) fixed on both sides of the positioning frame plate (3), one of the support plates (71) is threadedly plugged with an adjustment screw (73) rotatably connected to one of the connecting plates (72), the top end of the adjustment screw (73) is fixedly connected to a rotary plate (731), the other support plate (71) is slidably plugged with a guide slide bar (74) fixedly connected to the other connecting plate (72), the top end of the guide slide bar (74) is fixedly connected to a baffle (741).

Images