Sampling device for rapid in-vitro sampling of animal entity tissues
By designing a bimodal structure sampling needle, the cumbersome animal sample collection process in the prior art is solved, real-time and rapid collection of high-quality animal tissue samples on site, and the sampling process is simplified.
Patent Information
- Application Number
- CN202421166078.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-24
- Publication Date
- 2025-04-18
- Estimated Expiration
- 2034-05-24
AI Technical Summary
The prior art cannot simplify the animal sample collection process, and it is difficult to achieve real-time and rapid collection of high-quality animal tissue samples on site. In addition, traditional methods have cumbersome procedures for animal transportation and laboratory anatomy.
A sample needle in hollow tubular form, including a bimodal design of the main and secondary needle peaks, is designed to quickly acquire animal solid tissue under minimally invasive conditions and apply negative or positive pressure through the syringe to prevent tissue from falling off and effectively discharge the tissue.
The rapid acquisition of animal solid tissue under minimally invasive conditions is achieved, the sampling process is simplified, the cumbersome procedures in traditional methods are avoided, and high-quality biological samples are provided for subsequent genetic and molecular biological analysis.
Smart Images
Figure CN222765193U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of biological / medical equipment, and in particular to a sampling device for rapid in vitro sampling of animal entity tissues. Background Art
[0002] In clinical medical diagnosis, there is a method called needle biopsy, which is used to collect tissue samples from living bodies. Clinically, there are two types of sampling needles used for needle biopsy, one is a thick needle used to obtain solid tissue masses for pathological examination, and the other is a fine needle used to obtain a small amount of cells and body fluids from soft tissues. The thick needle method is a more complex sleeve-type structure that cuts and obtains solid tissues. It can obtain solid tissue samples, but it has limitations such as high sampling environment requirements, greater invasiveness, local anesthesia, and high costs. The fine needle method is relatively simple, similar to a small-diameter injection needle, which obtains a small amount of cells and body fluids from soft tissues through multiple pumping and negative pressure of the needle tube, but its limitation is that it is not suitable for obtaining solid tissue samples. At present, neither the thick needle method nor the fine needle method has been applied to tissue sampling of environmental animals, but the thick needle method is occasionally used for pathological examinations in veterinary medicine.
[0003] Vertebrates include five major animal groups: mammals, birds, reptiles, amphibians, and fish. The sampling of biological tissues of different groups of vertebrates in the natural environment is an important part of subsequent laboratory genetic species identification, biodiversity surveys, and protection. The sampling of biological tissues of economic animals in animal husbandry and fisheries is an important part of animal gene banks, reproduction, and market anti-counterfeiting traceability. At present, there is no unified method for collecting biological samples from various types of vertebrates. The traditional method is to take the captured animals to the experimental base and kill them, and then obtain animal tissues through the animal dissection process, and store them under low temperature conditions for subsequent molecular biological analysis. Therefore, the collection of biological samples from animals is a very cumbersome process, and different groups of animals have their own particularities that must be taken into account.
[0004] How to simplify the animal sample collection process, realize the real-time and rapid collection of high-quality animal tissue samples on site, and preserve them at room temperature is a realistic and important need. Utility Model Content
[0005] Technical issues
[0006] In view of this, the utility model provides a sampling device for rapid in vitro sampling of animal solid tissues, which can overcome the defect of being unable to capture animal solid tissues while maintaining the advantages of simple fine needle operation and small tissue trauma, providing a feasible solution for real-time and rapid sampling of various vertebrates.
[0007] Solution
[0008] In order to solve the above technical problems, the utility model provides the following technical solutions:
[0009] The utility model provides a sampling device for rapid in vitro sampling of animal entity tissues, comprising a sampling needle in a hollow tubular form, wherein the sampling needle comprises a tube portion and a tip portion that are integrally connected, wherein the tip portion comprises a main needle peak and a secondary needle peak that form a V-shaped incision, wherein the secondary needle peak is shorter than the main needle peak and is used to block or force sample tissue to enter the tube cavity from the V-shaped incision formed by the main and secondary needle peaks.
[0010] Furthermore, the length of the secondary peak is 1 / 10 to 1 / 2 of the length of the primary peak, preferably 1 / 5 to 1 / 3.
[0011] Furthermore, a blocking structure for blocking the sample from falling is provided on the tube wall of the tube portion.
[0012] Furthermore, the blocking structure is a protrusion protruding into the lumen, or spaced through holes, or a combination of protrusions and through holes, which is used to prevent the sample from falling.
[0013] Furthermore, the protrusions are annular protrusions or spaced protrusions.
[0014] Furthermore, the blocking structure is 2 to 5 mm away from the tip.
[0015] Furthermore, the sampling needle is a thin needle, and the outer diameter of the needle tube is 0.9-0.6mm.
[0016] Furthermore, the sampling needle is modified from a fine needle of international standard model G20-G23.
[0017] Furthermore, the outer walls of the main and auxiliary needle peaks are integrally formed with the tube portion.
[0018] Furthermore, it also includes a needle plug connected to the sampling needle tube; the needle plug is made of transparent material.
[0019] Beneficial Effects
[0020] (1) The utility model is a single-tube main and auxiliary double-peak (thin) needle design, which can quickly take samples, which is different from the existing thick needle method and thin needle method used in clinical medicine / veterinary needle examination. The double-peak design of the main and auxiliary needle peaks avoids the complex sleeve structure, strict operating conditions and large tissue trauma in the clinical thick needle method, and overcomes the defect that the clinical fine needle method cannot collect solid tissue. The double-peak design of the main and auxiliary needle peaks can obtain trace animal subcutaneous muscle tissue or other types of solid tissue in real time and quickly under minimally invasive conditions. The principle is that the double-peak design of the main and auxiliary needle peaks can efficiently capture animal subcutaneous muscle tissue under the synergistic effect of the large and small needle peaks when the needle is inserted, and the blocking structure at the base of the needle peak and the negative pressure provided by the syringe can prevent the tissue from falling off when the needle is removed, greatly improving the efficiency of each double-peak fine needle under minimally invasive conditions. The animal tissue obtained by the double-peak design of the main and auxiliary needle peaks can be effectively discharged from the needle tube into a designated sterile collection tube containing preservation fluid by the positive pressure applied by the syringe for subsequent experimental processing.
[0021] (2) The dual-peak structural design of the main and auxiliary needle peaks of the utility model can be used for real-time and rapid sampling in the natural environment, greatly simplifying the steps of environmental animal biological sampling and avoiding the cumbersome procedures of animal transportation and laboratory dissection in traditional methods.
[0022] (3) The dual-peak structure design of the main and auxiliary needle peaks of the utility model can realize two sampling modes, one is the rapid in vitro acupuncture sampling of dead animals, and the other is the minimally invasive acupuncture sampling of living animals. In particular, the minimally invasive sampling of living animals will create a biodiversity-friendly sampling mode. It is also suitable for rapid tissue sampling of the five major vertebrate groups, providing a unified standardized sampling scheme for the sampling of different groups of animals.
[0023] (4) The rapid or living tissue samples obtained by the dual-peak structure design of the main and auxiliary needle peaks of the utility model can be stored in a suitable sample preservation solution in a timely manner, thereby maintaining the original state of nucleic acid molecules in animal tissues to the maximum extent, and providing high-quality biological samples for subsequent genetic and molecular biology detection.
[0024] The above description is only an overview of the technical solution of the utility model. In order to more clearly understand the technical means of the utility model and implement it according to the contents of the specification, and at the same time to make the above and other purposes, technical features and advantages of the utility model easier to understand, one or more preferred embodiments are listed below and described in detail with the accompanying drawings as follows. BRIEF DESCRIPTION OF THE DRAWINGS
[0025] One or more embodiments are exemplarily described by pictures in the corresponding drawings, and these exemplified descriptions do not constitute limitations on the embodiments. Elements with the same reference numerals in the drawings represent similar elements, and unless otherwise stated, the figures in the drawings do not constitute proportional limitations.
[0026] Figure 1 It is a structural schematic diagram of an embodiment of the sampling device for rapid in vitro sampling of animal entity tissue of the utility model, wherein the lower figure is an enlarged view of the part within the circle in the upper figure.
[0027] Figure 2 yes Figure 1 AA section view.
[0028] Figure 3 It is a structural schematic diagram and BB cross-sectional view of a second embodiment of a sampling device for rapid in vitro sampling of animal solid tissue;
[0029] Figure 4 It is a structural schematic diagram and CC cross-sectional view of a third embodiment of a sampling device for rapid in vitro sampling of animal solid tissue;
[0030] Figure 5 It is a structural schematic diagram and CC cross-sectional view of the fourth embodiment of the sampling device for rapid in vitro sampling of animal entity tissue.
[0031] Description of main reference numerals:
[0032] 1- tip portion, 11- main needle peak, 12- secondary needle peak, 2- tube portion, 21- annular protrusion, 21'- spaced protrusion, 21"- through hole, 3- needle plug. DETAILED DESCRIPTION
[0033] The specific implementation modes of the present invention are described in detail below in conjunction with the accompanying drawings, but it should be understood that the protection scope of the present invention is not limited by the specific implementation modes.
[0034] Unless explicitly stated otherwise, throughout the specification and claims, the term “comprise” or variations such as “include” or “comprising”, etc., will be understood to include the stated elements or components but not to exclude other elements or components.
[0035] In this document, for the convenience of description, spatial relative terms such as "below", "below", "down", "above", "above", "upper", etc. may be used to describe the relationship between one element or feature and another element or feature in the accompanying drawings. It should be understood that the spatial relative terms are intended to include different orientations of the object in use or operation in addition to the orientation depicted in the figure. For example, if the object in the figure is turned over, the element described as being "below" or "below" other elements or features will be oriented "above" the element or feature. Therefore, the exemplary term "below" can include both below and above. Objects may also have other orientations (rotated 90 degrees or other orientations) and the spatial relative terms used in this document should be interpreted accordingly.
[0036] In this document, the terms "first", "second", etc. are used to distinguish two different elements or parts, and are not used to limit a specific position or relative relationship. In other words, in some embodiments, the terms "first", "second", etc. can also be interchangeable.
[0037] like Figures 1 to 5 As shown, the utility model provides a sampling device for rapid in vitro sampling of animal solid tissues, including a sampling needle in a hollow tubular form, the sampling needle including a tube portion 2 and a tip portion 1 that are integrally connected, the tip portion including a main needle peak 11 and a secondary needle peak 12 that form a V-shaped incision, the secondary needle peak 12 being shorter than the main needle peak 11, and being used to block or force the sample tissue to enter the tube cavity from the V-shaped incision formed by the main needle peak 11 and the secondary needle peak 12.
[0038] When the utility model is used, the main needle peak and the auxiliary needle peak are directly and slowly inserted into the subcutaneous muscle tissue from the body of a freshly killed or living vertebrate animal at a confirmed location (appendages, back or abdominal muscle groups) for obtaining subcutaneous muscle tissue from outside the body, and the auxiliary needle peak provides a blocking effect for the tissue pierced by the main needle peak, forcing part of the tissue to more effectively enter the lumen at the V-shaped incision (i.e., between the two needle peaks). The needle is extracted from the subcutaneous tissue 1-3 times, and negative pressure is applied by a syringe at the same time. When the needle is withdrawn, negative pressure is maintained and the needle is withdrawn slowly. The tissue in the needle is transferred to a sterilized 1.5 ml microtube, and the syringe applies positive pressure to remove the tissue.
[0039] An improved solution is that the length of the secondary needle peak 12 can be 1 / 10 to 1 / 2 of the length of the main needle peak 11, preferably 1 / 5 to 1 / 3.
[0040] In order to prevent the captured tissue from falling out of the lumen, a blocking structure for blocking the sample from falling is provided on the wall of the tube portion 2 .
[0041] Further optionally, the blocking structure is a protrusion protruding into the lumen, or a spaced through hole 21" ( Figure 4 ), or a combination of protrusions and through holes ( Figure 5), used to prevent samples from falling.
[0042] Some possible structures include: the protrusion is an annular protrusion 21 ( Figure 1 ), spaced protrusions 21'( Figure 3 ).
[0043] Furthermore, the blocking structure is 2 to 5 mm away from the tip, which can more effectively prevent tissue from falling off.
[0044] Furthermore, the sampling needle is a thin needle, and the outer diameter of the needle tube is 0.9-0.6mm.
[0045] Furthermore, the sampling needle is modified from the fine needle of the international standard model G20-G23.
[0046] Furthermore, the outer walls of the main needle peak 11 and the auxiliary needle peak 12 are integrally formed with the tube portion.
[0047] Furthermore, it also includes a needle plug 3 connected to the sampling needle tube part 2; the needle plug 3 is made of transparent material, which is convenient for observing tissue samples. The needle plug and the nipple interface adopt a standard syringe size, which is convenient for use with syringes of different sizes and provides positive and negative pressures.
[0048] The specific implementation of the utility model:
[0049] 1) Operation steps: Confirm the location of subcutaneous muscle tissue (leg, back or abdominal muscle group) on the body of a freshly killed or living vertebrate animal, and slowly insert the sampling needle (main and secondary double-peaked fine needle) directly into the subcutaneous muscle tissue from the outside of the body. The needle is extracted from the subcutaneous tissue 1-3 times, and negative pressure is applied through the syringe. When the needle is withdrawn, negative pressure is maintained and withdrawn slowly. The tissue in the needle is transferred to a sterile 1.5ml microtube, and positive pressure is applied to the syringe to remove the tissue.
[0050] 2) Comparison of sampling efficiency between the primary and secondary double-peak fine needle method and the single-peak fine needle of the same specification: The primary and secondary double-peak fine needle can successfully obtain subcutaneous muscle tissue from the body through the above operation, with 1-3 extractions each time. In contrast, the standard single-peak fine needle of the same specification generally cannot obtain subcutaneous muscle tissue through the above operation, or occasionally obtains a small amount of tissue after more than 15-20 extractions.
[0051] 3) Examples of different types of vertebrates: The primary and secondary double-peak fine needle method can effectively obtain in vitro subcutaneous muscle tissue in representative animals, including mice (mammals), parrots and pigeons (birds), tiger frogs (amphibians), various fish (fish), etc.
[0052] 4) The primary and secondary double-peak fine needle method for obtaining animal tissues can be successfully used in various genetic and molecular biological analyses.
[0053] The foregoing description of specific exemplary embodiments of the utility model is for the purpose of illustration and illustration. These descriptions are not intended to limit the utility model to the precise form disclosed, and it is clear that many changes and variations can be made based on the above teachings. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the utility model and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the utility model and various different options and changes. Any simple modifications, equivalent changes and modifications made to the above exemplary embodiments should fall within the scope of protection of the utility model.
Claims
1. A sampling device for rapid in vitro sampling of animal solid tissue, characterized in that: The sampling needle comprises a hollow tubular form, which comprises a tube portion and a tip portion which are integrally connected, wherein the tip portion comprises a main needle peak and a secondary needle peak which form a V-shaped incision, wherein the secondary needle peak is shorter than the main needle peak and is used to block or force the sample tissue to enter the tube cavity from the V-shaped incision formed by the main and secondary needle peaks.
2. The sampling device for rapid in vitro sampling of animal solid tissue according to claim 1, characterized in that: The length of the secondary needle peak is 1 / 10 to 1 / 2 of the length of the main needle peak.
3. The sampling device for rapid in vitro sampling of animal solid tissue according to claim 1, characterized in that: The length of the secondary needle peak is 1 / 5 to 1 / 3 of the length of the main needle peak.
4. The sampling device for rapid in vitro sampling of animal solid tissue according to any one of claims 1 to 3, characterized in that: A blocking structure for blocking the sample from falling is arranged on the tube wall of the tube portion.
5. The sampling device for rapid in vitro sampling of animal solid tissue according to claim 4, characterized in that: The blocking structure is a protrusion protruding toward the lumen, or spaced through holes, or a combination of protrusions and through holes, and is used to prevent the sample from falling.
6. The sampling device for rapid in vitro sampling of animal solid tissue according to claim 5, characterized in that: The protrusions are annular protrusions or spaced protrusions.
7. The sampling device for rapid in vitro sampling of animal solid tissue according to claim 5, characterized in that: The blocking structure is 2 to 5 mm away from the tip.
8. The sampling device for rapid in vitro sampling of animal solid tissue according to any one of claims 1 to 3, characterized in that: The sampling needle is a thin needle with an outer diameter of 0.9-0.6 mm; And / or, the sampling needle is modified from a fine needle of international standard model G20-G23.
9. The sampling device for rapid in vitro sampling of animal solid tissue according to any one of claims 1 to 3, characterized in that: The outer walls of the main and auxiliary needle peaks are integrally formed with the tube portion.
10. The sampling device for rapid in vitro sampling of animal solid tissue according to any one of claims 1 to 3, characterized in that: It also includes a needle plug connected to the sampling needle tube; the needle plug is made of transparent material.