Plasma separating device

Abstract

The plasma separation device comprises a shell and a pressing piece, the shell comprises an extrusion cavity used for containing whole blood, and a liquid outlet is formed in the extrusion cavity; the pressing piece comprises a pressure applying end, the pressure applying end is configured to be capable of being inserted into the extrusion cavity to compress the volume of the extrusion cavity, a filtering membrane is fixedly arranged in the extrusion cavity, the extrusion cavity is compressed by means of the pressure applying end to compress the whole blood in the extrusion cavity to the filtering membrane, and therefore the blood plasma flowing to the liquid outlet is filtered out. Whole blood can be pressed through the pressing piece, so that plasma is extruded to penetrate through the filter membrane and then is discharged from the liquid outlet, plasma separation and filtration are achieved, operation is rapid and convenient, and consumed time is short.

Description

Technical Field

[0001] The utility model relates to the technical field of filtering devices, in particular to a plasma separation device. Background Art

[0002] The existing separation of plasma and serum is done by centrifugation, which is complicated and time-consuming. Under laboratory conditions, separation of plasma from whole blood is usually achieved by centrifuging the blood at 2000-4000r / min for about 20 minutes. After the blood is centrifuged, the solid components of the blood sink in the sediment, while the supernatant is composed of plasma, and the plasma is extracted from the supernatant. The separation process of plasma requires the use of equipment such as centrifuges, which is cumbersome and time-consuming. Utility Model Content

[0003] Therefore, the utility model provides a plasma separation device to solve the above-mentioned problems.

[0004] To achieve the above purpose, the technical solution provided by the utility model is as follows:

[0005] A plasma separation device comprises a shell and a pressing piece, wherein the shell comprises an extrusion chamber for containing whole blood, and the extrusion chamber is provided with a discharge port; the pressing piece comprises a pressure-applying end, and the pressure-applying end is configured to be inserted into the extrusion chamber to compress the volume of the extrusion chamber, and a filter membrane is fixedly arranged in the extrusion chamber. The extrusion chamber is compressed by means of the pressure-applying end to push the whole blood in the extrusion chamber toward the filter membrane, thereby filtering out the plasma flowing toward the discharge port.

[0006] Optionally, the extrusion cavity is a columnar cavity, and the shape of the pressure-applying end is a columnar shape matching the extrusion cavity, and the pressure-applying end is inserted into the extrusion cavity so that the insertion gap between the two is sealed.

[0007] Optionally, the shell also includes an annular hollow peripheral wall portion and a bottom wall portion fixedly arranged at one end of the peripheral wall portion, the peripheral wall portion and the bottom wall portion enclose the extrusion cavity, the liquid discharge port is arranged on the bottom wall portion, and the filter membrane is pressed and sealed between the peripheral wall portion and the bottom wall portion.

[0008] Optionally, a sealing portion annularly surrounding the liquid discharge port is provided at one end of the peripheral wall portion facing the bottom wall portion or at one end of the bottom wall portion facing the peripheral wall portion, and the compression seal is achieved through the sealing portion.

[0009] Optionally, a drainage groove is provided on the bottom wall portion, one end of the drainage groove is connected to the drainage port, and the other end of the drainage groove extends in a direction away from the drainage port.

[0010] Optionally, a plurality of drainage grooves are provided, and the plurality of drainage grooves expand radially with the drainage port as the center.

[0011] Optionally, the shell further includes a guide cavity, the inner diameter of the guide cavity is larger than the extrusion cavity, the guide cavity is connected with the extrusion cavity to form a stepped hole, the guide cavity and the extrusion cavity are transitioned by a step surface, the pressing member includes a guide portion fixedly connected to the pressure end, the guide portion is configured to be inserted into and slidably connected to the guide cavity, the step surface forms a limit on the insertion stroke of the pressure end to achieve quantitative filtration of plasma.

[0012] Optionally, the guide portion is provided with an air relief groove on its circumferential outer wall, and both ends of the air relief groove extend to both ends of the axial direction of the guide portion respectively; or, the guide cavity is provided with an air relief groove on its circumferential inner wall, and both ends of the air relief groove extend to both ends of the axial direction of the guide cavity respectively.

[0013] Optionally, the shell further includes a liquid storage chamber and a channel, wherein the channel connects the extrusion chamber and the liquid storage chamber, and a port of the channel serves as the liquid discharge port so that plasma can flow into the liquid storage chamber through the channel.

[0014] Optionally, the liquid storage chamber is configured to narrow from top to bottom.

[0015] Optionally, the shell also includes a bottom plate, the extrusion chamber and the liquid storage chamber are both arranged on the upper end surface of the bottom plate, the lower end surface of the bottom plate is provided with a through groove connecting the extrusion chamber and the liquid storage chamber, and a bottom membrane for sealing the through groove is fixedly provided on the bottom plate so that the space between the bottom membrane and the through groove forms the channel.

[0016] Optionally, a connecting piece is also included, wherein the connecting piece connects the shell and the pressing piece.

[0017] The technical solution provided by the utility model has the following beneficial effects: by applying external force to the pressing piece, the volume of the extrusion cavity can be compressed, so that the plasma is squeezed through the filter membrane and discharged from the discharge port, thereby realizing the separation and filtration of the plasma, and the operation is quick and convenient, and the time consumption is short; the sealing part can be pressed on the circumferential edge of the filter membrane to prevent blood from leaking from the circumferential edge of the filter membrane to the discharge port, thereby ensuring the filtration and separation effect of the plasma; the provision of the groove can increase the effective filtration area of ​​the plasma on the filter membrane and improve the filtration efficiency of the plasma; the provision of the stepped surface can limit the insertion stroke of the pressure-applying end, thereby realizing quantitative filtration of the plasma. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 is an overall schematic diagram of the plasma separation device in this embodiment;

[0019] Figure 2 is a cross-sectional view of the plasma separation device in this embodiment;

[0020] Figure 3Schematic diagram of the explosion of the plasma separation device in this embodiment.

[0021] Explanation of the reference numerals: 1. Shell; 11. Extrusion chamber; 12. Liquid discharge port; 13. Peripheral wall; 14. Bottom wall; 15. Sealing portion; 16. Drainage groove; 17. Guide chamber; 18. Step surface; 2. Pressing member; 21. Pressure end; 22. Guide portion; 23. Air release groove; 3. Filter membrane; 4. Bottom plate; 5. Liquid storage chamber; 6. Channel; 7. Bottom membrane; 8. Connecting member. DETAILED DESCRIPTION

[0022] To further illustrate the various embodiments, the present invention provides drawings. These drawings are part of the disclosure of the present invention, which are mainly used to illustrate the embodiments and can be used in conjunction with the relevant descriptions in the specification to explain the operating principles of the embodiments. With reference to these contents, a person of ordinary skill in the art should be able to understand other possible implementations and advantages of the present invention. The components in the figures are not drawn to scale, and similar component symbols are generally used to represent similar components.

[0023] The utility model is now further described in conjunction with the accompanying drawings and specific implementation methods.

[0024] Reference Figure 1-3 A plasma separation device is used to filter out plasma from whole blood. The plasma separation device includes a shell 1 and a pressing piece 2. For ease of description, the description is based on the direction of the plasma separation device in a static state. The shell 1 includes an extrusion chamber 11 for containing whole blood, and the extrusion chamber 11 is provided with a discharge port 12. The pressing piece 2 includes a pressure-applying end 21, and the pressure-applying end 21 is configured to be inserted into the extrusion chamber 11 to compress the volume of the extrusion chamber 11. A filter membrane 3 is fixedly provided in the extrusion chamber 11. The extrusion chamber 11 is compressed by means of the pressure-applying end 21 to direct the whole blood in the extrusion chamber 11 toward the filter membrane 3, thereby filtering out the plasma flowing to the discharge port 12. The entire plasma separation and filtration process is simple and convenient to operate and takes a short time.

[0025] Among them, the filter membrane 3 can use a polyethersulfone membrane with a thickness of 0.3-0.4mm to separate and filter plasma. The polyethersulfone membrane is a hydrophilic filter membrane with a highly asymmetric pore structure, that is, the hydrophilic blood can automatically diffuse and be evenly distributed on the filter membrane; the asymmetric pore structure of the filter membrane is that one side has a large pore diameter and the other side has a small pore diameter, with a minimum pore diameter of about 1.8um, and the effect of filtering red blood cells is significant.

[0026] Specifically, the shape of the pressure-applying end 21 is a columnar shape matching the extrusion cavity 11, and the pressure-applying end 21 is inserted into the extrusion cavity 11 to seal the insertion gap therebetween. In this way, it can be ensured that after the whole blood in the extrusion cavity 11 is squeezed by external force, the plasma can only be squeezed to penetrate toward the filter membrane 3, thereby achieving separation and filtration of the plasma.

[0027] Reference Figure 2 Furthermore, the housing 1 also includes an annular hollow peripheral wall portion 13 and a bottom wall portion 14 fixedly arranged at one end of the peripheral wall portion 13. The peripheral wall portion 13 and the bottom wall portion 14 enclose an extrusion chamber 11, and the peripheral wall portion 13 and the bottom wall portion 14 are connected and fixed by ultrasonic welding. The discharge port 12 is arranged on the bottom wall portion 14, and the filter membrane 3 is pressed and sealed between the peripheral wall portion 13 and the bottom wall portion 14, so as to ensure that after the whole blood is squeezed, it will all be filtered through the filter membrane 3 to separate the plasma. Specifically, one end of the peripheral wall portion 13 facing the bottom wall portion 14 or one end of the bottom wall portion 14 facing the peripheral wall portion 13 is provided with a sealing portion 15 annularly surrounding the discharge port 12, and the sealing portion 15 is pressed and sealed. In this embodiment, the sealing portion 15 is arranged at one end of the peripheral wall portion 13 toward the bottom wall portion 14 to achieve a compression seal on the circumferential edge of the filter membrane 3, which can prevent whole blood from seeping from the circumferential edge of the filter membrane 3 to the drain port 12, thereby ensuring the filtering and separation effect on plasma.

[0028] Since the filter membrane 3 is attached to the bottom wall 14, in order to expand the area of ​​plasma passing through the filter membrane 3 and improve the plasma separation efficiency, a drainage groove 16 is provided on the bottom wall 14, one end of which is connected to the drain port 12 and the other end extends in a direction away from the drain port 12. Figure 3 As shown, there are several drainage grooves 16, and the drainage grooves 16 expand radially with the discharge port 12 as the center; in this way, while increasing the area of ​​plasma passing through the filter membrane 3, the inner wall of the bottom wall portion 14 can still provide sufficient support for the filter membrane 3 to prevent the filter membrane 3 from rupturing due to excessive local pressure.

[0029] Reference Figure 2 and Figure 3 The housing 1 also includes a guide cavity 17, the inner diameter of which is larger than the extrusion cavity 11, the guide cavity 17 is connected to the extrusion cavity 11 to form a stepped hole, the guide cavity 17 and the extrusion cavity 11 are transitioned by a stepped surface 18, the pressing member 2 includes a guide portion 22 fixedly connected to the pressing end 21, the guide portion 22 is configured to be inserted into and slidably connected to the guide cavity 17, the stepped surface 18 forms a limit to the insertion stroke of the guide portion 22, and can limit the maximum stroke of the pressing end 21 in the extrusion cavity 11; that is, when the lower end surface of the guide portion 22 conflicts with the stepped surface 18, the pressing end 21 cannot continue to move downward, and the insertion stroke of the pressing end 21 in the extrusion cavity 11 is constant, thereby achieving quantitative extrusion of whole blood in the extrusion cavity 11 and quantitatively controlling the filtration amount of plasma.

[0030] In addition, when the pressure end 21 just enters the extrusion chamber 11, an annular cavity is formed between the lower end surface of the guide portion 22 and the step surface. In order to discharge the air in the cavity, an air relief groove 23 is provided on the outer peripheral side wall of the guide portion 22. The two ends of the air relief groove 23 extend to the two ends of the axial direction of the guide portion 22, and there are multiple air relief grooves 23 arranged along the circumference of the guide portion 22, so that the air in the cavity can be quickly discharged from each air relief groove 23, so that it is easy to drive the pressure end 21 to squeeze the whole blood. In other embodiments, the air relief groove 23 can also be provided on the circumferential inner wall of the guide chamber 17, and the two ends of the air relief groove 23 extend to the two ends of the axial direction of the guide chamber 17. The air relief groove 23 can also be arranged on the circumferential inner wall of the guide chamber 17 and the circumferential outer wall of the guide portion 22 at the same time.

[0031] Reference Figure 2 and Figure 3 The housing 1 further includes a liquid storage chamber 5 and a channel 6. The channel 6 connects the extrusion chamber 11 with the liquid storage chamber 5. One port of the channel 6 serves as a liquid discharge port 12, so that the plasma can flow into the liquid storage chamber 5 through the channel 6. The plasma flows out from the liquid discharge port 12, flows into the liquid storage chamber 5 after passing through the channel 6, and the plasma can be extracted as needed in the liquid storage chamber 5 by a pipette, a glass capillary, a quantitative pipette, etc., which is convenient for extracting the plasma; and the amount of filtered plasma can be visually seen in the liquid storage chamber 5, so as to facilitate the control of the amount of filtered plasma. Among them, the liquid storage chamber 5 is narrowed from top to bottom. In this embodiment, the liquid storage chamber 5 is in the shape of a conical funnel. In this way, when the plasma in the liquid storage chamber 5 is extracted, the residual amount of plasma will be less.

[0032] The housing 1 further comprises a bottom plate 4, wherein the extrusion cavity 11 and the liquid storage cavity 5 are both arranged on the upper end surface of the bottom plate 4, and a through groove connecting the extrusion cavity 11 and the liquid storage cavity 5 is provided on the lower end surface of the bottom plate 4, and a bottom film 7 for blocking the through groove is fixedly provided on the bottom plate 4, so that a channel 6 is formed in the space between the bottom film 7 and the through groove. In this embodiment, a part of the bottom plate 4 is used as the bottom wall portion 14, and the other part is used as the bottom of the liquid storage cavity 5. That is, the bottom plate 4 is integrally injection molded, and a liquid storage cavity 5 is directly injection molded on the upper end surface of the bottom plate 4; wherein the side of the bottom plate 4 away from the injection cavity can be fixed to the lower end surface of the peripheral wall portion 13 by ultrasonic welding, as the bottom wall portion 14, and the extrusion cavity 11 is surrounded by the peripheral wall portion 13. A through groove connecting the extrusion cavity 11 and the liquid storage cavity 5 is provided on the lower end surface of the bottom plate 4, and a bottom film 7 for blocking the through groove is fixedly provided on the bottom plate 4, so that a channel 6 is formed in the space between the bottom film 7 and the through groove. The bottom film 7 can be fixed to the lower end surface of the bottom plate 4 by hot pressing.

[0033] In addition, the plasma separation device further includes a connecting member 8, which connects the housing 1 and the pressing member 2. Specifically, the connecting member 8 is a flexible connecting strip, one end of which is connected to the pressing member, and the other end is connected to the bottom plate 4 of the housing 1, so that the pressing member 2 is not easily lost. The connecting strip can be integrally injection molded with the same material as the bottom plate 4.

[0034] When separating and filtering the plasma, whole blood is added into the extrusion chamber 11 and allowed to stand for 0.5-2 minutes. After the whole blood soaks the filter membrane 3, the pressing piece 2 is covered and pressed down. The whole blood passes through the filter membrane 3 to filter out the plasma, and the plasma flows into the liquid storage chamber 5 through the channel 6 at the bottom of the bottom plate 4. At this time, the plasma can be extracted as needed using a pipette, a glass capillary, a quantitative pipette, etc. The whole process is easy to operate and takes a short time.

[0035] Although the present invention has been specifically demonstrated and described in conjunction with the preferred embodiments, those skilled in the art should understand that various changes may be made to the present invention in form and detail without departing from the spirit and scope of the present invention as defined in the appended claims, all of which are within the scope of protection of the present invention.

Claims

1. A plasma separation device, characterized in that: The invention comprises a shell and a pressing member, wherein the shell comprises an extrusion chamber for containing whole blood, and the extrusion chamber is provided with a discharge port; the pressing member comprises a pressure-applying end, and the pressure-applying end is configured to be inserted into the extrusion chamber to compress the volume of the extrusion chamber, and a filter membrane is fixedly arranged in the extrusion chamber. The extrusion chamber is compressed by means of the pressure-applying end to push the whole blood in the extrusion chamber toward the filter membrane, thereby filtering out the plasma flowing toward the discharge port.

2. A plasma separation device according to claim 1, characterized in that: The extrusion cavity is a columnar cavity, and the shape of the pressure-applying end is a columnar shape matching the extrusion cavity. The pressure-applying end is inserted into the extrusion cavity so that the insertion gap between the two is sealed.

3. A plasma separation device according to claim 1, characterized in that: The shell also includes an annular hollow peripheral wall portion and a bottom wall portion fixedly arranged at one end of the peripheral wall portion, the peripheral wall portion and the bottom wall portion enclose the extrusion cavity, the liquid discharge port is arranged on the bottom wall portion, and the filter membrane is pressed and sealed between the peripheral wall portion and the bottom wall portion.

4. A plasma separation device according to claim 3, characterized in that: One end of the peripheral wall portion facing the bottom wall portion or one end of the bottom wall portion facing the peripheral wall portion is provided with a sealing portion which is annularly surrounding the liquid discharge port, and the compression seal is achieved through the sealing portion.

5. A plasma separation device according to claim 3, characterized in that: The bottom wall portion is provided with a drainage groove, one end of which is connected to the liquid discharge port, and the other end of which extends in a direction away from the liquid discharge port.

6. A plasma separation device according to claim 5, characterized in that: A plurality of drainage grooves are provided, and the plurality of drainage grooves expand radially with the liquid discharge port as the center.

7. A plasma separation device according to claim 1, characterized in that: The shell also includes a guide cavity, the inner diameter of which is larger than that of the extrusion cavity, the guide cavity is connected to the extrusion cavity to form a stepped hole, the guide cavity and the extrusion cavity are transitioned by a stepped surface, the pressing member includes a guide portion fixedly connected to the pressure end, the guide portion is configured to be inserted into and slidably connected to the guide cavity, and the stepped surface forms a limit on the insertion stroke of the pressure end to achieve quantitative filtration of plasma.

8. A plasma separation device according to claim 7, characterized in that: The guide portion is provided with an air relief groove on its circumferential outer wall, and both ends of the air relief groove extend to both ends of the guide portion in the axial direction; or, the guide cavity is provided with an air relief groove on its circumferential inner wall, and both ends of the air relief groove extend to both ends of the guide cavity in the axial direction.

9. A plasma separation device according to claim 1, characterized in that: The shell also includes a liquid storage chamber and a channel, wherein the channel connects the extrusion chamber and the liquid storage chamber, and a port of the channel serves as the liquid discharge port so that plasma can flow into the liquid storage chamber through the channel.

10. A plasma separation device according to claim 9, characterized in that: The shell also includes a bottom plate, the extrusion cavity and the liquid storage cavity are both arranged on the upper end surface of the bottom plate, the lower end surface of the bottom plate is provided with a through groove connecting the extrusion cavity and the liquid storage cavity, and a bottom membrane for blocking the through groove is fixedly arranged on the bottom plate so that the space between the bottom membrane and the through groove forms the channel.

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