Cell culture constant pressure instrument

Abstract

The utility model discloses a constant pressure instrument for cell culture, which belongs to the technical field of cell culture and comprises a culture box, a culture component is mounted on the inner side of the culture box, a box cover is arranged at the top of the culture box and above the culture component, and the culture box is connected with the box cover through two bolts. A pressure pump is installed on one side of the incubator, a pressure relief assembly is installed on the side, away from the pressure pump, of the incubator, and a sterilization cleaning device is arranged below the incubator; the sterilizing and cleaning device is arranged, so that the constant pressure instrument can perform certain high-temperature sterilizing and cleaning work on the interior of the incubator through cooperation of a fan, an electric heating rod and other structures when needed, and a worker can move the constant pressure instrument through brake wheels when needed; therefore, the working requirements under different conditions can be better met, and the practicability of the constant pressure instrument is further improved.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell culture, and in particular relates to a constant pressure instrument for cell culture. Background Art

[0002] Cells are the basic units of the morphological structure and life activities of organisms. It is generally believed that cells are the smallest unit of the human body. They are composed of many smaller structures with their own functions. Although human cells vary in size, all cells are small.

[0003] Chinese patent application No. 202322098444.0 discloses a cell culture constant pressure instrument, including an incubator; a pressure pump for supplying pressure inside the incubator, the pressure pump being installed on one side of the incubator; a pressure relief assembly for maintaining the internal pressure of the incubator, the pressure relief assembly including a pressure relief pipe, a mounting rod being fixedly connected to the inner wall of the pressure relief pipe, and two symmetrically arranged mounting slide rods being slidably connected to the mounting rod. The utility model can not only squeeze the sealing slider when the pressure inside the incubator is too high by setting a pressure relief assembly, but also when the pressure inside the incubator reaches a certain value, the sealing slider moves to the other side of the pressure relief hole, so that the pressure inside the incubator can be relieved, thereby ensuring that the pressure inside the incubator is constant, and at the same time, the culture rack is driven to move up and down by the electric cylinder, so that it is convenient to put in and out cells, thereby improving the practicality of the utility model.

[0004] 1. The above-mentioned patent has the problem that it is inconvenient to sterilize and clean the inside of the incubator and it is inconvenient to move, thereby reducing the practicality of the constant pressure instrument; 2. The above-mentioned patent has the problem that it cannot prevent the pressure relief work from being carried out when necessary, thereby making it difficult to meet the work needs in different situations, thereby reducing the working capacity of the constant pressure instrument. Utility Model Content

[0005] In order to solve the problems raised in the above background technology, the utility model provides a cell culture constant pressure instrument, which has the characteristics of strong practicability and high working capacity.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a cell culture constant pressure instrument, comprising an incubator, a culture component is installed on the inner side of the incubator, a box cover is arranged on the top of the incubator and above the culture component, the incubator and the box cover are connected by two bolts, a pressure pump is installed on one side of the incubator, a pressure relief component is installed on the side of the incubator away from the pressure pump, a sterilization and cleaning device is arranged below the incubator, and a closed isolation device is installed on the side of the incubator away from the pressure pump and outside the pressure relief component.

[0007] Preferably, the sterilization and cleaning device includes a bottom box, a fan, an electric heating rod, a filter assembly and a connecting pipe. The bottom box is arranged below the incubator, a plurality of fans are installed between the incubator and the bottom box, a plurality of connecting pipes are installed between the incubator and the bottom box and on one side of the plurality of fans, a plurality of electric heating rods are installed on the inner side of the bottom box, and a filter assembly is arranged on one side of the plurality of electric heating rods.

[0008] Preferably, the sterilization and cleaning device further comprises a brake wheel, and a plurality of brake wheels are installed below the bottom box.

[0009] Preferably, the filter assembly includes a filter cloth, an observation window and a blocking block. The filter cloth is installed on the inner side of the bottom box and at a position on one side of the multiple heating rods. The blocking block is installed through a thread on one side of the bottom box and at a position on the side of the filter cloth away from the multiple heating rods. An observation window is installed on the inner side of the blocking block.

[0010] Preferably, the closed isolation device includes a screw, a knob, a movable plate and a closing sleeve, the screw is installed through a bearing on a side of the incubator away from the pressure pump and located above the pressure relief assembly, the knob is installed on a side of the screw away from the incubator, the movable plate is installed through a thread on the outside of the screw and located on a side of the pressure relief assembly away from the incubator, and the closing sleeve is installed on a side of the movable plate close to the pressure relief assembly and located outside the pressure relief assembly.

[0011] Preferably, the closed isolation device further comprises a guide rod, and the guide rod is installed on a side of the incubator close to the movable plate and above the screw rod.

[0012] Compared with the prior art, the beneficial effects of the utility model are:

[0013] 1. The utility model provides a sterilization and cleaning device so that the constant pressure instrument can perform certain high-temperature sterilization and cleaning work on the inside of the incubator through the cooperation of structures such as a fan and an electric heating rod when necessary, and the staff can move the position of the constant pressure instrument through various brake wheels when necessary, so as to better meet the work needs in different situations, thereby improving the practicality of the constant pressure instrument.

[0014] 2. The utility model provides a closed isolation device, so that the staff can, when necessary, carry out a certain closed isolation of the pressure relief component through the cooperation of the screw rod and the closed sleeve and other structures, thereby preventing the pressure relief work from being carried out, better meeting the work needs in different situations, and thus improving the working capacity of the constant pressure instrument. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a three-dimensional diagram of the utility model;

[0016] Figure 2 It is a three-dimensional cutaway view of the utility model;

[0017] Figure 3 It is a three-dimensional cutaway view of the sterilization and cleaning device of the utility model;

[0018] Figure 4 It is a three-dimensional cutaway view of the closed isolation device of the utility model.

[0019] In the figure: 1. incubator; 2. pressure pump; 3. sterilization and cleaning device; 31. bottom box; 32. fan; 33. electric heating rod; 34. filter assembly; 341. filter cloth; 342. observation window; 343. blocking block; 35. brake wheel; 36. connecting pipe; 4. incubator assembly; 5. pressure relief assembly; 6. closed isolation device; 61. screw rod; 62. guide rod; 63. knob; 64. moving plate; 65. closing sleeve; 7. bolt; 8. box cover. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0021] Example 1

[0022] See also Figure 1-4 The utility model provides the following technical solutions: a cell culture constant pressure instrument, comprising an incubator 1, a culture component 4 is installed on the inner side of the incubator 1, a box cover 8 is arranged on the top of the incubator 1 and above the culture component 4, the incubator 1 and the box cover 8 are connected by two bolts 7, a pressure pump 2 is installed on one side of the incubator 1, a pressure relief component 5 is installed on the side of the incubator 1 away from the pressure pump 2, a sterilization and cleaning device 3 is arranged below the incubator 1, and a closed isolation device 6 is installed on the side of the incubator 1 away from the pressure pump 2 and outside the pressure relief component 5.

[0023] Specifically, the sterilization and cleaning device 3 includes a bottom box 31, a fan 32, an electric heating rod 33, a filter assembly 34 and a connecting pipe 36. The bottom box 31 is arranged below the incubator 1, and multiple fans 32 are installed between the incubator 1 and the bottom box 31. Multiple connecting pipes 36 are installed between the incubator 1 and the bottom box 31 and on one side of the multiple fans 32. Multiple electric heating rods 33 are installed on the inner side of the bottom box 31, and a filter assembly 34 is arranged on one side of the multiple electric heating rods 33.

[0024] By adopting the above technical solution, the constant pressure instrument can perform certain high-temperature sterilization work on the inside of the incubator 1 through the cooperation of structures such as the fan 32 and the electric heating rod 33 when necessary, so as to better meet the working needs in different situations.

[0025] Specifically, the sterilization and cleaning device 3 further includes a brake wheel 35 , and a plurality of brake wheels 35 are installed below the bottom box 31 .

[0026] By adopting the above technical solution, the staff can move the position of the constant pressure instrument to a certain extent through each brake wheel 35 when necessary, thereby reducing the difficulty of moving the constant pressure instrument and alleviating the labor burden of the staff.

[0027] Specifically, the filter assembly 34 includes a filter cloth 341, an observation window 342 and a blocking block 343. The filter cloth 341 is installed on the inner side of the bottom box 31 and at a position on one side of the multiple heating rods 33. The blocking block 343 is installed through threads on one side of the bottom box 31 and at a position on the side of the filter cloth 341 away from the multiple heating rods 33. The observation window 342 is installed on the inner side of the blocking block 343.

[0028] By adopting the above technical solution, the constant pressure instrument can perform certain cleaning work on the inside of the incubator 1 through the cooperation of structures such as the fan 32 and the filter cloth 341 when necessary, so as to better meet the working needs in different situations.

[0029] When the present embodiment is used, when the staff needs to use the constant pressure instrument for culture work, the staff pushes the constant pressure instrument, moves the constant pressure instrument to the working position through the brake wheels 35 in the sterilization and cleaning device 3, unscrews the two bolts 7 to remove the box cover 8, and then places the cells to be cultured on the culture component 4, and then reinstalls the box cover 8 on the culture box 1 through the two bolts 7, and then operates the pressure pump 2 to pressurize the culture box 1. When the pressure is too high, the pressure relief component 5 can relieve the pressure, so that the inside of the culture box 1 maintains a constant pressure. After the cell culture is completed, unscrew the two bolts 7 again to remove the box cover 8, take out the cells to be cultured, and then reinstall the box cover 8 on the culture box 1 through the two bolts 7, and start each fan 32. And each electric heating rod 33, under the action of each fan 32, the air in the incubator 1 and the dust and other impurities therein pass through each connecting pipe 36 into the bottom box 31, and then the impurities are blocked by the filter cloth 341 in the filter assembly 34, and the air passes through the filter cloth 341 and is heated by each electric heating rod 33, and then the hot air passes through each fan 32 and returns to the incubator 1, so as to sterilize and disinfect the inside of the incubator 1. When the sterilization and cleaning work are completed, turn off each fan 32 and each electric heating rod 33, unscrew the blocking block 343, take out the dust and other impurities blocked by the filter cloth 341, and then install the blocking block 343 back through the thread, and the work is completed. When necessary, the staff can observe the inside of the bottom box 31 through the observation window 342.

[0030] Example 2

[0031] The present embodiment is different from the embodiment 1 in that the closed isolation device 6 comprises a screw rod 61, a knob 63, a movable plate 64 and a closed sleeve 65; the screw rod 61 is installed via a bearing on a side of the incubator 1 away from the pressure pump 2 and located above the pressure relief assembly 5; the knob 63 is installed on a side of the screw rod 61 away from the incubator 1; the movable plate 64 is installed via a thread on the outside of the screw rod 61 and located on a side of the pressure relief assembly 5 away from the incubator 1; the closed sleeve 65 is installed on a side of the movable plate 64 close to the pressure relief assembly 5 and located on the outside of the pressure relief assembly 5.

[0032] By adopting the above technical solution, the staff can, when necessary, seal and isolate the pressure relief assembly 5 through the cooperation of the screw rod 61 and the sealing sleeve 65, thereby preventing the pressure relief work from being carried out and better meeting the work needs in different situations.

[0033] Specifically, the closed isolation device 6 further includes a guide rod 62 , and the guide rod 62 is installed on one side of the incubator 1 close to the movable plate 64 and above the screw rod 61 .

[0034] By adopting the above technical solution, the constant pressure instrument can guide and limit the movement of the movable plate 64 to a certain extent through the guide rod 62, thereby preventing the movable plate 64 from rotating and deviating during the movement process, ensuring the normal operation.

[0035] When the present embodiment is used, when it is necessary to prevent the pressure relief work from being carried out, the staff turns the knob 63 in the closed isolation device 6, thereby driving the screw rod 61 to rotate. The screw rod 61 is threadedly connected to the movable plate 64, and the movable plate 64 is slidingly connected to the guide rod 62, so the screw rod 61 drives the movable plate 64 and the closed sleeve 65 thereon to move until the closed sleeve 65 seals and isolates the pressure relief component 5, thereby preventing the pressure relief component 5 from performing the pressure relief work, increasing the pressure in the incubator 1, and better meeting the work needs in different situations.

[0036] The structure and principle of the incubator 1, the pressure pump 2, the culture component 4, and the pressure relief component 5 in the utility model have been disclosed in a cell culture constant pressure instrument disclosed in Chinese patent application No. 202322098444.0. The working principle is that by setting a pressure relief component, the sealing slider can be squeezed when the pressure inside the incubator is too high, and when the pressure inside the incubator reaches a certain value, the sealing slider moves to the other side of the pressure relief hole, so that the pressure inside the incubator can be relieved, thereby ensuring that the pressure inside the incubator is constant. At the same time, the culture rack is driven to move up and down by the electric cylinder, so that it is convenient to put cells in and out, thereby improving the practicality of the utility model.

[0037] The working principle and use process of the utility model are as follows: when the staff needs to use the constant pressure instrument for culture work, the staff pushes the constant pressure instrument, moves the constant pressure instrument to the working position through the brake wheels 35 in the sterilization and cleaning device 3, unscrews the two bolts 7 to remove the box cover 8, and then places the cells to be cultured on the culture component 4, and then reinstalls the box cover 8 on the culture box 1 through the two bolts 7, and then operates the pressure pump 2 to pressurize the culture box 1. When the pressure is too high, the pressure relief component 5 can perform pressure relief work, so that the inside of the culture box 1 maintains a constant pressure. After the cell culture is completed, unscrew the two bolts 7 again to remove the box cover 8, take out the cells to be cultured, and then reinstall the box cover 8 on the culture box 1 through the two bolts 7, start each fan 32 and each electric heating rod 33, and under the action of each fan 32, the air in the culture box 1 and the impurities such as dust therein pass through each connecting pipe 36 into the bottom box 31, and then the impurities are blocked by the filter cloth 341 in the filter component 34, and the air passes through The filter cloth 341 is heated by each electric heating rod 33, and then the hot air passes through each fan 32 and returns to the incubator 1, thereby sterilizing and disinfecting the interior of the incubator 1. After the sterilization and cleaning work are completed, the fans 32 and the electric heating rods 33 are turned off, the blocking blocks 343 are unscrewed, the dust and other impurities blocked by the filter cloth 341 are taken out, and the blocking blocks 343 are installed back through the threads. The work is completed. When necessary, the staff can observe the interior of the bottom box 31 through the observation window 342; when it is necessary to prevent the pressure relief work from being carried out, the staff turns the knob 63 in the sealing isolation device 6, thereby driving the screw rod 61 to rotate, the screw rod 61 is threadedly connected to the movable plate 64, and the movable plate 64 is slidably connected to the guide rod 62, so the screw rod 61 drives the movable plate 64 and the sealing sleeve 65 thereon to move until the sealing sleeve 65 seals and isolates the pressure relief component 5, thereby preventing the pressure relief component 5 from performing the pressure relief work, enhancing the pressure in the incubator 1, and better meeting the work needs in different situations.

[0038] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell culture constant pressure instrument, comprising an incubator, characterized in that: A culture assembly is installed inside the incubator, a box cover is arranged on the top of the incubator and above the culture assembly, the incubator and the box cover are connected by two bolts, a pressure pump is installed on one side of the incubator, a pressure relief assembly is installed on the side of the incubator away from the pressure pump, a sterilization and cleaning device is arranged below the incubator, and a closed isolation device is installed on the side of the incubator away from the pressure pump and outside the pressure relief assembly; The sterilization and cleaning device includes a bottom box, a fan, an electric heating rod, a filter assembly and a connecting pipe. The bottom box is arranged below the incubator, a plurality of fans are installed between the incubator and the bottom box, a plurality of connecting pipes are installed between the incubator and the bottom box and at a position on one side of the plurality of fans, a plurality of electric heating rods are installed on the inner side of the bottom box, and a filter assembly is arranged on one side of the plurality of electric heating rods.

2. A cell culture constant pressure instrument according to claim 1, characterized in that: The sterilization and cleaning device also includes a brake wheel, and a plurality of brake wheels are installed below the bottom box.

3. A cell culture constant pressure instrument according to claim 1, characterized in that: The filter assembly includes a filter cloth, an observation window and a blocking block. The filter cloth is installed on the inner side of the bottom box and at a position on one side of the multiple heating rods. The blocking block is installed on one side of the bottom box and at a position away from the multiple heating rods by means of threads. The observation window is installed on the inner side of the blocking block.

4. A cell culture constant pressure instrument according to claim 1, characterized in that: The closed isolation device includes a screw, a knob, a movable plate and a closed sleeve. The screw is installed through a bearing on a side of the incubator away from the pressure pump and above the pressure relief assembly. The knob is installed on a side of the screw away from the incubator. The movable plate is installed through a thread on the outside of the screw and located on the side of the pressure relief assembly away from the incubator. The closed sleeve is installed on a side of the movable plate close to the pressure relief assembly and outside the pressure relief assembly.

5. A cell culture constant pressure instrument according to claim 4, characterized in that: The closed isolation device also includes a guide rod, which is installed on a side of the incubator close to the movable plate and above the screw rod.

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