Temperature measurement calibration tool for nucleic acid detection equipment

Abstract

The utility model discloses a temperature measurement calibration tool for nucleic acid detection equipment, and the temperature measurement calibration tool comprises a lower cover plate which is provided with a mounting hole; the circuit board is arranged above the lower cover plate; the temperature acquisition assembly comprises a heat conduction part, a mounting part and a temperature information acquisition part, and the heat conduction part is used for being in contact with a heat dissipation surface on a to-be-detected standard part of the nucleic acid detection equipment; the temperature information acquisition part is arranged in the heat conduction part, is electrically connected with the circuit board and is used for detecting the temperature of the heat dissipation surface; and the mounting piece is inserted into the mounting hole and is detachably connected with the heat conducting piece. The temperature measurement calibration tool for the nucleic acid detection equipment has the advantages of being simple in structure and beneficial to improving the temperature measurement accuracy of the nucleic acid detection equipment.

Description

Technical Field

[0001] The present application belongs to the field of medical testing technology, and specifically relates to a temperature measurement and calibration tool for nucleic acid testing equipment. Background Art

[0002] With the development of automated medical testing technology (such as PCR gene amplification technology), temperature control modules have become the technical core of nucleic acid testing equipment (such as PCR instruments), and temperature control-related parameters have become important performance indicators of nucleic acid testing equipment (such as PCR instruments). However, in the prior art, most of the nucleic acid testing equipment (such as PCR instruments) manufactured by manufacturers have not been calibrated, resulting in certain differences in the temperature measurement results of nucleic acid testing equipment manufactured by various manufacturers, making it difficult to ensure that the manufactured nucleic acid testing equipment can meet the design requirements. Utility Model Content

[0003] The purpose of the present application is to provide a temperature measurement and calibration tool for nucleic acid detection equipment, which has the advantages of simple structure and is conducive to improving the temperature measurement accuracy of nucleic acid detection equipment.

[0004] In order to achieve the above-mentioned purpose, the present application provides a temperature measurement and calibration tool for a nucleic acid detection device, the temperature measurement and calibration tool comprising:

[0005] A lower cover plate, wherein a mounting hole is formed on the lower cover plate;

[0006] A circuit board is arranged above the lower cover plate;

[0007] The temperature collection component includes a heat conductor, a mounting component and a temperature information collection component. The heat conductor is used to contact the heat dissipation surface of the standard component to be tested on the nucleic acid detection equipment; the temperature information collection component is arranged inside the heat conductor and electrically connected to the circuit board, and is used to detect the temperature of the heat dissipation surface; the mounting component is inserted in the mounting hole and is detachably connected to the heat conductor.

[0008] In an embodiment of the utility model, a first thread is provided at the top of the heat conducting member, and a second thread for matching with the first thread is provided at the bottom of the mounting member.

[0009] In an embodiment of the utility model, a downwardly recessed cavity is formed on the lower cover plate, and a supporting cylinder portion extending upward from the bottom wall of the cavity is also formed on the lower cover plate, a mounting hole is formed on the supporting cylinder portion, and the mounting member includes a disc portion and a columnar portion located below the disc portion and inserted into the mounting hole, and the disc portion is arranged above the supporting cylinder portion.

[0010] In an embodiment of the utility model, a rotation limiting portion for limiting the rotation of the mounting member is also formed on the lower cover plate.

[0011] In an embodiment of the utility model, the rotation limiting portion is in the shape of a flat plate and protrudes upward from the top surface of the supporting cylinder portion, and a limiting groove for the rotation limiting portion to extend laterally is formed on the disc portion.

[0012] In an embodiment of the utility model, the temperature measurement and calibration tool further includes a pressing pad which is arranged below the circuit board and is used to pre-press the mounting member.

[0013] In an embodiment of the present invention, the material of the pressing pad is silica gel.

[0014] In an embodiment of the utility model, a hollow portion is formed on the lower pressure pad.

[0015] In an embodiment of the utility model, a protrusion is formed on the mounting member, which protrudes upward from the highest position of the cavity and is used to abut against the lower pressure pad.

[0016] In an embodiment of the utility model, the temperature measurement and calibration tool further includes an upper cover plate which is arranged above the circuit board and is detachably connected to the lower cover plate.

[0017] Through the above technical scheme, it can be known that the temperature measurement and calibration tool includes: a lower cover plate, on which a mounting hole is formed; a circuit board, which is arranged above the lower cover plate; a temperature acquisition component, including a heat conductor, a mounting part and a temperature information acquisition part, the heat conductor is used to contact the heat dissipation surface on the standard part to be tested of the nucleic acid detection equipment; the temperature information acquisition part is arranged inside the heat conductor and electrically connected to the circuit board, and is used to detect the temperature of the heat dissipation surface; the mounting part is inserted in the mounting hole and is detachably connected to the heat conductor. The temperature measurement and calibration tool has a simple structure and can effectively calibrate the nucleic acid detection equipment to ensure that the manufactured nucleic acid detection equipment can meet the design requirements; the detachable connection between the mounting part and the heat conductor can facilitate the operator to place the temperature sensor into the accommodating cavity according to actual use requirements, and is also convenient for replacing damaged mounting parts or heat conductors, which is beneficial to extending the overall service life of the temperature measurement and calibration tool, and is also beneficial to improving the practicality and economy of the temperature measurement and calibration tool.

[0018] Other features and advantages of the embodiments of the present application will be described in detail in the subsequent detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The accompanying drawings are used to provide a further understanding of the embodiments of the present application and constitute a part of the specification. Together with the following specific embodiments, they are used to explain the embodiments of the present application, but do not constitute a limitation on the embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without creative work. In the drawings:

[0020] Figure 1 It is a cross-sectional schematic diagram of the temperature measurement and calibration tooling in the embodiment of the utility model;

[0021] Figure 2 This is a schematic diagram of the overall structure of the temperature measurement and calibration tooling in the embodiment of the utility model;

[0022] Figure 3 This is a schematic diagram of the composition of the lower cover plate and the temperature collection component in the embodiment of the utility model (first perspective);

[0023] Figure 4 This is a schematic diagram of the composition of the lower cover plate and the temperature collection component in the embodiment of the utility model (second viewing angle);

[0024] Figure 5 This is a schematic diagram of the structure of the lower cover plate in the embodiment of the utility model;

[0025] Figure 6 This is a schematic diagram of the components of the heat conducting member and the mounting member in the embodiment of the utility model;

[0026] Figure 7 It is a schematic diagram of the structure of the downward pressure pad in the embodiment of the utility model.

[0027] Description of Reference Numerals

[0028] 1-lower cover; 101-mounting hole; 102-concave cavity; 103-support cylinder; 104-rotation limiter; 2-circuit board; 3-temperature acquisition assembly; 301-heat conducting member; 302-mounting member; 3021-disc; 3022-columnar portion; 3023-limiting groove; 3024-protrusion; 4-lower pressure pad; 401-hollow portion; 5-upper cover. DETAILED DESCRIPTION

[0029] The specific implementation of the present application is described in detail below in conjunction with the accompanying drawings. It should be understood that the specific implementation described here is only used to illustrate and explain the present application, and is not used to limit the present application.

[0030] The embodiments of the present application provide a novel temperature measurement and calibration tool for nucleic acid detection equipment, such as Figure 1-Figure 2 As shown, the temperature measurement and calibration tooling includes:

[0031] A lower cover plate 1, wherein a mounting hole 101 is formed on the lower cover plate 1;

[0032] A circuit board 2 is arranged above the lower cover plate 1;

[0033] The temperature acquisition component 3 includes a heat conductor 301, a mounting component 302 and a temperature information acquisition component. The heat conductor 301 is used to contact the heat dissipation surface of the standard component to be tested on the nucleic acid detection equipment; the temperature information acquisition component is arranged inside the heat conductor 301 and is electrically connected to the circuit board 2, and is used to collect temperature information of the heat dissipation surface; the mounting component 302 is inserted into the mounting hole 101 and is detachably connected to the heat conductor 301.

[0034] Specifically, the nucleic acid detection equipment in this embodiment can be selected as a PCR instrument, and the standard part to be tested in this embodiment can be selected as a carrier of the PCR instrument (not shown in the figure). The carrier is made of aluminum alloy and is formed with a downwardly concave detection cavity. A heating element (such as a Peltier) for preheating it is provided below the carrier. When the heating element (not shown in the figure) performs the preheating function, the cavity wall of the detection cavity will heat up; the circuit board 2 can be selected as a PCB board, and there are two FPC (Flexible Printed Circuit) connection sockets on the PCB board for external connection; the number of mounting holes 101 and the number of temperature acquisition components 3 are both multiple, and the temperature detection results of each temperature acquisition component 3 can be compared with each other, which is conducive to improving the calibration accuracy of the temperature measurement and calibration tool; the heat conductor 301 is conical with the tip facing downward. The manufacturing material of the heat conductor 301 includes brass and nickel (or other materials with good thermal conductivity), and nickel is plated on the outer surface of the brass. The above-mentioned arrangement can make the heat conductor 301 have good thermal conductivity. When the temperature measurement and calibration tool performs temperature calibration, the bottom end of the heat conductor 301 It is in contact with the bottom wall of the detection cavity (that is, the bottom wall of the detection cavity is the heat dissipation surface), and the angle of the conical surface of the heat conductor 301 is 17.5°; a accommodating cavity is formed inside the heat conductor 301, and the temperature information acquisition component (not shown in the figure) can be selected as a temperature sensor and is arranged in the accommodating cavity, and the heat conductor 301 can transfer the temperature of the heat dissipation surface to the detection end of the temperature detector; the manufacturing material of the mounting component 302 includes plastic (or other materials with good heat resistance), so that the mounting component 302 has good heat resistance, and it is also beneficial to reduce the production cost of the temperature measurement and calibration tooling.

[0035] Before using the temperature measurement and calibration tool in this embodiment to perform temperature calibration, the heating element preheats the carrier; when using the temperature measurement and calibration tool to perform temperature calibration, the temperature measurement and calibration tool is placed on the carrier, each heat conductor 301 is respectively in each detection cavity, and the bottom end of each heat conductor 301 is in contact with the bottom wall of each detection cavity, the heating element heats the carrier, and the heat conductor 301 transmits the temperature of the heat dissipation surface to the temperature sensor, and the temperature sensor transmits the measured temperature information to the circuit board 2. The chip on the circuit board 2 calculates the heating rate (and / or cooling rate) of the carrier according to the above temperature information and calibrates the nucleic acid detection equipment according to the above heating rate (and / or cooling rate). The temperature measurement and calibration tool has a simple structure and can effectively calibrate the nucleic acid detection equipment to ensure that the manufactured nucleic acid detection equipment can meet the design requirements. Furthermore, the detachable connection (such as threaded connection or snap-on connection) between the mounting component 302 and the heat conductor 301 in this embodiment can facilitate the operator to place the temperature sensor into the accommodating cavity according to actual usage requirements, and also facilitate the replacement of damaged mounting components 302 or heat conductors 301, which is beneficial to extending the overall service life of the temperature measurement and calibration tooling, and is also beneficial to improving the practicality and economy of the temperature measurement and calibration tooling.

[0036] Furthermore, the temperature measurement and calibration tool also includes a filler that fills the accommodating cavity, and the filler can be thermal grease. When installing the temperature sensor, first fill the accommodating cavity with thermal grease, and then place the temperature sensor inside the accommodating cavity so that it is in full contact with the thermal grease, so that heat can be effectively conducted between the two.

[0037] In one embodiment of the present invention, the top of the heat conducting member 301 is provided with a first thread (such as an external thread), and the bottom of the mounting member 302 is provided with a second thread (such as an internal thread) for matching with the first thread. That is, in this embodiment, the heat conducting member 301 and the mounting member 302 are connected in a detachable manner by threaded matching. This connection method has the advantages of convenient installation and disassembly, strong connection stability, good sealing and long service life.

[0038] In one embodiment of the utility model, a downwardly recessed cavity 102 is formed on the lower cover plate 1, and a supporting cylinder portion 103 extending upward from the bottom wall of the cavity 102 is also formed on the lower cover plate 1. The mounting hole 101 is formed on the supporting cylinder portion 103. The mounting member 302 includes a disc portion 3021 and a columnar portion 3022 located below the disc portion 3021 and inserted into the mounting hole 101. The disc portion 3021 is arranged above the supporting cylinder portion 103.

[0039] Specifically, a second thread (such as an internal thread) is formed at the bottom end of the columnar portion 3022, that is, the columnar portion 3022 is connected to the heat conductor 301 through a threaded connection. The height range of the support cylinder 103 is 2mm-4mm. In this embodiment, the height of the support cylinder 103 is preferably 3mm. The above arrangement can effectively increase the coaxiality of the support cylinder 103 and the mounting member 302, and increase the installation fit of the mounting member 302 on the lower cover plate 1, thereby effectively preventing the temperature acquisition component 3 from shaking or deflecting after installation, so as to avoid affecting the temperature information collection effect and the temperature calibration effect; the arrangement of the disc portion 3021 can prevent the temperature acquisition component 3 from falling from the mounting hole 101 and separating from the lower cover plate 1, thereby ensuring the reliability of the temperature measurement and calibration tooling.

[0040] In one embodiment of the present invention, Figure 3 and Figure 5 As shown, a rotation limiting portion 104 for limiting the rotation of the mounting member 302 is also formed on the lower cover plate 1, wherein the rotation limiting portion 104 is in the shape of a plate and protrudes upward from the top surface of the supporting cylinder portion 103, and a limiting groove 3023 for the rotation limiting portion 104 to extend laterally is formed on the disc portion 3021.

[0041] Specifically, the temperature sensor and the circuit board 2 are connected through a signal line (one end of the signal line passes through the mounting piece 302 and is connected to the circuit board 2). The setting of the rotation limit portion 104 and the limit groove 3023 can prevent the temperature acquisition component 3 from rotating during operation, thereby preventing the signal line from being driven to rotate and being damaged or affecting the signal transmission function; further, the number of the rotation limit portions 104 is two, and the two rotation limit portions 104 are arranged on two radially opposite sides of the support cylinder 103. Correspondingly, the number of the limit grooves 3023 is also two, and the positions of the two limit grooves 3023 correspond one-to-one to the positions of the two rotation limit portions 104. In the event that one rotation limit portion 104 is damaged, the other rotation limit portion 104 can still be used normally, and will not affect the rotation limiting effect on the mounting piece 302. That is, the above-mentioned setting is conducive to further strengthening the rotation limiting effect on the mounting piece 302.

[0042] In one embodiment of the present invention, the temperature measurement and calibration tool further includes a pressing pad 4 (such as a pressure pad 4) disposed below the circuit board 2 and used to press the mounting member 302. Figure 7 shown).

[0043] Specifically, the pressing pad 4 is fixed under the circuit board 2. When there are multiple temperature acquisition components 3 (such as 16) in the temperature measurement and calibration tooling, due to installation and manufacturing errors, after all the temperature acquisition components 3 are installed, there may be a few (such as 1, 2 or 3, etc.) temperature acquisition components 3 in a skewed state. The setting of the pressing pad 4 allows a certain amount of pre-interference between the circuit board 2 and the mounting member 302. When assembling the temperature measurement and calibration tooling, the pressing pad 4 can apply downward pressure to the mounting member 302, and the skewed temperature acquisition component 3 as a whole is restored to a vertical state under the action of the above-mentioned downward pressure, so that all the temperature acquisition components 3 can be in a vertical state and at the same height, thereby avoiding external factors from having an adverse effect on the temperature information acquisition effect of the temperature acquisition component 3, which is beneficial to further ensure the calibration effectiveness of the temperature measurement and calibration tooling.

[0044] In one embodiment of the present invention, the pressing pad 4 is made of silicone, which makes it flexible and has a buffering property, thereby preventing the pressing pad 4 from damaging the mounting member 302 and / or the circuit board 2 when pressing the mounting member 302. Furthermore, in this embodiment, the pressing pad 4 can also be made of other flexible materials (such as polyurethane).

[0045] In one embodiment of the utility model, a hollow portion 401 is formed on the lower pressure pad 4. Specifically, in this embodiment, the number of the hollow portions 401 is multiple (such as 3), and the multiple hollow portions 401 are distributed at intervals in the horizontal direction. In the longitudinal direction, the multiple hollow portions 401 are located in the middle position of the lower pressure pad 4. The above arrangement can not only ensure that the lower pressure pad 4 can perform the downward pressure function on each mounting part 302, but also save the manufacturing materials of the lower pressure pad 4, which is conducive to realizing the lightweight design of the temperature measurement and calibration tooling, and is also conducive to reducing the production cost of the temperature measurement and calibration tooling.

[0046] In one embodiment of the present invention, the mounting member 302 is formed with a protrusion 3024 (such as a protrusion 3024) protruding upward from the highest position of the cavity 102 and used to abut against the lower pressure pad 4. Figure 4 and Figure 6 Specifically, the protrusion 3024 is cylindrical and is located above the disc portion 3021. The protrusion 3024 has a simple structure, which is easy to process and manufacture, and can fully ensure that the mounting member 302 can abut against the pressing pad 4 up and down, thereby ensuring that the pressing pad 4 can perform the pressing function on each mounting member 302.

[0047] In one embodiment of the present invention, the temperature measurement and calibration tool further includes an upper cover plate 5 disposed above the circuit board 2 and detachably connected to the lower cover plate 1. Specifically, the upper cover plate 5 is fixed to the circuit board 2 and the lower pressure pad 4, and the arrangement of the upper cover plate 5 can effectively protect the internal components of the temperature measurement and calibration tool (such as the circuit board 2 and the lower pressure pad 4, etc.); the upper cover plate 5 and the lower cover plate 1 can be detachably connected by a snap-fit ​​structure or a bolt assembly, which is convenient for repairing or replacing the internal components of the temperature measurement and calibration tool when they are damaged, and is conducive to further extending the overall service life of the temperature measurement and calibration tool.

[0048] In the description of the present application, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of the present application, "plurality" means at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0049] In this application, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection, or communication with each other; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0050] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0051] Although the embodiments of the present application have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations on the present application. Ordinary technicians in this field can change, modify, replace and modify the above embodiments within the scope of the present application.

Claims

1. A temperature measurement and calibration tool for nucleic acid detection equipment, characterized in that: The temperature measurement and calibration tooling comprises: A lower cover plate (1), wherein a mounting hole (101) is formed on the lower cover plate (1); A circuit board (2) is arranged above the lower cover plate (1); A temperature collection component (3) comprises a heat conducting component (301), a mounting component (302) and a temperature information collection component, wherein the heat conducting component (301) is used to contact the heat dissipation surface of a standard component to be tested of a nucleic acid detection device; the temperature information collection component is arranged inside the heat conducting component (301) and is electrically connected to the circuit board (2) for collecting temperature information of the heat dissipation surface; the mounting component (302) is inserted into the mounting hole (101) and is detachably connected to the heat conducting component (301).

2. The temperature measurement and calibration tool for nucleic acid detection equipment according to claim 1, characterized in that: The top of the heat conducting member (301) is provided with a first thread, and the bottom of the mounting member (302) is provided with a second thread for matching with the first thread.

3. The temperature measurement and calibration tool for nucleic acid detection equipment according to claim 1, characterized in that: The lower cover plate (1) is formed with a downwardly recessed cavity (102), and the lower cover plate (1) is also formed with a support cylinder portion (103) extending upward from the bottom wall of the cavity (102); the mounting hole (101) is formed on the support cylinder portion (103); the mounting member (302) comprises a disc portion (3021) and a columnar portion (3022) located below the disc portion (3021) and inserted into the mounting hole (101); the disc portion (3021) is arranged above the support cylinder portion (103).

4. The temperature measurement and calibration tool for nucleic acid detection equipment according to claim 3, characterized in that: The lower cover plate (1) is also formed with a rotation limiting portion (104) for limiting the rotation of the mounting member (302).

5. The temperature measurement and calibration tool for nucleic acid detection equipment according to claim 4, characterized in that: The rotation limiting portion (104) is in the shape of a flat plate and protrudes upward from the top surface of the supporting cylinder portion (103); a limiting groove (3023) is formed on the disc portion (3021) for the rotation limiting portion (104) to extend laterally therein.

6. The temperature measurement and calibration tool for nucleic acid detection equipment according to claim 3, characterized in that: The temperature measurement and calibration tool also includes a pressing pad (4) arranged below the circuit board (2) and used for pre-pressing the mounting member (302).

7. The temperature measurement and calibration tool for nucleic acid detection equipment according to claim 6, characterized in that: The material of the pressing pad (4) is silica gel.

8. The temperature measurement and calibration tool for nucleic acid detection equipment according to claim 6, characterized in that: A hollow portion (401) is formed on the lower pressure pad (4).

9. The temperature measurement and calibration tool for nucleic acid detection equipment according to claim 6, characterized in that: The mounting member (302) is formed with a protruding portion (3024) which protrudes upward from the highest position of the concave cavity (102) and is used to abut against the lower pressure pad (4).

10. The temperature measurement and calibration tool for nucleic acid detection equipment according to any one of claims 1 to 9, characterized in that: The temperature measurement and calibration tool also includes an upper cover plate (5) which is arranged above the circuit board (2) and is detachably connected to the lower cover plate (1).

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