Polypeptide cracking reactor

Abstract

The utility model relates to the technical field of polypeptide synthesis, in particular to a polypeptide cracking reactor which comprises a reaction tank body and a filtering device, and a discharging opening is formed in the lower portion of the reaction tank body; the filtering device comprises a shell, a rotating mechanism, a feeding hole, a liquid outlet and at least two filtering barrels; the feeding hole is formed in the top surface of the shell and is connected with the discharging hole; the rotating mechanism is inserted into the filtering device from the upper part; the filtering barrel is arranged on the rotating mechanism and can rotate to the position below the feeding opening through the rotating mechanism. After the cracking reaction is completed, all materials are put into the filtering device through the discharging port to be filtered, filtrate is pumped out from the liquid outlet, filter residues are left in the filtering barrel, and by arranging the rotating mechanism, a new filtering barrel can be automatically switched to the position below the feeding port after one-time filtering for next-round filtering, so that the frequency of cleaning the filter residues by workers is reduced. And the reaction tank body can continuously work, so that the production efficiency is effectively improved.

Description

Technical Field

[0001] The present application relates to the technical field of polypeptide synthesis, and in particular to a polypeptide cleavage reactor. Background Art

[0002] The solid phase synthesis of peptides uses resin as an insoluble solid phase carrier, connects different amino acids to the solid phase carrier in sequence to obtain peptides, and then cuts the synthesized peptides from the carrier (resin), i.e., cleavage. Generally, after cleavage of the peptide, the cleavage liquid needs to be filtered and separated from the solid, and then the cleavage liquid is precipitated, dried, and other operations are performed.

[0003] The existing process is to release the lysate after the cleavage reaction, and then take out the remaining resin residue in the reactor, that is, the process of filtering and separating the lysate from the solid is carried out in the reactor. In this method, a filter plate needs to be set in the reactor. The presence of the filter plate will limit the setting of the stirring paddle. Even if a stirring paddle is set, the stirring effect is not good, resulting in incomplete cleavage reaction of some peptide resins. In addition, the filter plate has a small pore size, and the resin is precipitated on the filter plate, which will lead to a low liquid filtration rate, requiring a long waiting time, affecting production efficiency. Utility Model Content

[0004] The main purpose of the present application is to provide a polypeptide cleavage reactor, aiming to solve the current problem of low filtration rate after polypeptide cleavage, which affects production efficiency.

[0005] In order to achieve the above-mentioned object, the present application provides a polypeptide cracking reactor, comprising a reaction tank body and a filtering device, wherein a discharge port is provided at the bottom of the reaction tank body;

[0006] The filtering device comprises a housing, a rotating mechanism, a feed inlet, a liquid outlet and at least two filtering barrels;

[0007] The feed inlet is arranged on the top surface of the shell, and the feed inlet is connected to the discharge port;

[0008] The rotating mechanism is inserted into the filtering device from above; the filtering barrel is arranged on the rotating mechanism and can be rotated to below the feeding port through the rotating mechanism;

[0009] A sealing cover is provided on the top surface of the shell.

[0010] Optionally, the rotating mechanism includes a motor disposed above the housing and a rotating shaft disposed inside the housing;

[0011] The filter barrel comprises a mounting frame and an inner barrel, and the mounting frame is fixedly arranged on the rotating shaft;

[0012] The inner barrel is placed in the mounting frame.

[0013] Optionally, the rotating mechanism further comprises a rotating shaft support, and the rotating shaft support is arranged at the bottom of the filtering device;

[0014] The rotating shaft support is connected to the lower end of the rotating shaft through a bearing to support the rotating shaft.

[0015] Optionally, the polypeptide cleavage reactor further comprises a filtrate collecting container, and the filtrate collecting container is connected to the liquid outlet via a pipeline with a pump.

[0016] Optionally, a stirring paddle is provided in the reaction tank.

[0017] Optionally, the cross-section of the filter barrel is fan-shaped.

[0018] Optionally, a slag removal opening is provided below the sealing cover, and a diameter of the slag removal opening is larger than a diameter of the inner liner barrel.

[0019] Optionally, a support frame is further provided below the reaction tank body, and the reaction tank body is arranged on the support frame;

[0020] The filtering device is arranged in the space below the supporting frame.

[0021] Optionally, an air blowing port and a gas absorption device are provided on the outer shell of the filtering device.

[0022] Optionally, the side surface of the mounting frame is a grid plate, and the bottom surface of the mounting frame is a grid bar.

[0023] Compared with the prior art, the beneficial effects achieved by this application are:

[0024] The present application sets the filtering device under the reaction tank body. After the cracking reaction is completed, all materials are placed in the filtering device through the discharge port for filtration. The filtrate is extracted from the discharge port, and the filter residue is left in the filter barrel. By setting a rotating mechanism, a new filter barrel can be automatically switched to the bottom of the feed port after one filtration for the next round of filtration, reducing the frequency of staff cleaning the filter residue. After the materials in the reaction tank body are completely discharged, new materials can be immediately put in for cracking reaction, that is, the reaction tank body can work continuously without interruption, without wasting a long time waiting for filtration, effectively improving production efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying any creative work.

[0026] Figure 1 This is a schematic diagram of the structure of a polypeptide cleavage reactor in one embodiment of the present application;

[0027] Figure 2 This is a schematic diagram of the structure of a mounting frame in one embodiment of the present application;

[0028] Figure 3 This is a schematic diagram of the support frame installation in one embodiment of the present application.

[0029] Explanation of symbols: 1-reaction tank body, 101-stirring paddle, 102-discharge port, 2-housing, 201-feed port, 3-rotation mechanism, 301-rotation shaft, 4-filter barrel, 401-grid plate, 402-grid bar, 5-sealing cover, 6-rotation shaft support, 7-bearing, 8-liquid outlet, 9-filtrate collecting container, 10-air outlet, 11-gas absorption device, 12-support frame.

[0030] The realization of the purpose, functional features and advantages of this application will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0032] It should be noted that all directional indications in the embodiments of the present invention (such as up, down, left, right, front, back...) are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0033] In the present invention, unless otherwise clearly specified and limited, the terms "connection", "fixation", etc. should be understood in a broad sense. For example, "fixation" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0034] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the utility model, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the meaning of "and / or" appearing in the full text includes three parallel schemes. Taking "A and / or B" as an example, it includes scheme A, or scheme B, or a scheme that satisfies both A and B. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in this field to implement. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the utility model.

[0035] The solid phase synthesis of peptides uses resin as an insoluble solid phase carrier, connects different amino acids to the solid phase carrier in sequence to obtain peptides, and then cuts the synthesized peptides from the carrier (resin), i.e., cleavage. Generally, after cleavage of the peptide, the cleavage liquid needs to be filtered and separated from the solid, and then the cleavage liquid is precipitated, dried, and other operations are performed.

[0036] The existing process is to release the lysate after the cleavage reaction, and then take out the remaining resin residue in the reactor, that is, the process of filtering and separating the lysate from the solid is carried out in the reactor. In this method, a filter plate needs to be set in the reactor. The presence of the filter plate will limit the setting of the stirring paddle. Even if a stirring paddle is set, the stirring effect is not good, resulting in incomplete cleavage reaction of some peptide resins. In addition, the filter plate has a small pore size, and the resin is precipitated on the filter plate, which will lead to low liquid filtration efficiency, requiring a long waiting time, and the resin residue is prone to residual lysate, resulting in waste.

[0037] In order to solve the problems of the prior art, the present application provides a polypeptide cleavage reactor, see Figure 1 , comprising a reaction tank body 1 and a filtering device, wherein a discharge port 102 is provided below the reaction tank body 1;

[0038] The filtering device comprises a housing 2, a rotating mechanism 3, a feed port 201, a liquid outlet 8 and at least two filtering barrels 4;

[0039] The feed port 201 is disposed on the top surface of the housing 2, and the feed port 201 is connected to the discharge port 102;

[0040] The rotating mechanism 3 is inserted into the filtering device from above; the filtering barrel 4 is arranged on the rotating mechanism 3 and can be rotated to below the feed port 201 through the rotating mechanism 3;

[0041] A sealing cover 5 is provided on the top surface of the housing 2 .

[0042] The present application sets the filter device below the reaction tank body 1. After the cracking reaction is completed, all materials are placed in the filter device through the discharge port 102 for filtration, and the filtrate is extracted from the liquid outlet 8. The filter residue is left in the filter barrel 4, and by setting the rotating mechanism 3, the new filter barrel 4 can be automatically switched to the bottom of the feed port 201 after one filtration, and the next round of filtration is carried out, which reduces the frequency of staff cleaning the filter residue. And after the material in the reaction tank body 1 is completely discharged, new materials can be immediately put in for cracking reaction, that is, the reaction tank body 1 can work continuously without interruption, without wasting a long time waiting for filtration, and effectively improves production efficiency. It generally takes several hours to carry out cracking reaction in the reaction tank body 1, and this period of time can ensure that the filtrate is fully filtered and reduce the cracking liquid residue in the filter residue.

[0043] As an optional embodiment of the present application, the rotating mechanism 3 includes a motor disposed above the housing 2 and a rotating shaft 301 disposed inside the housing 2, and the rotating shaft 301 is connected to the output shaft of the motor; in order to facilitate the cleaning of the filter residue, the filter barrel 4 is designed to be two layers, that is, the filter barrel 4 includes a mounting frame and an inner barrel, and the mounting frame is fixedly disposed on the rotating shaft 301; the inner barrel is placed in the mounting frame. When the staff removes the filter residue, they only need to lift out the entire inner barrel, and then put another clean inner barrel into the mounting frame, which is convenient and quick to operate.

[0044] As an optional embodiment of the present application, the polypeptide cleavage reactor further includes a filtrate collection container 9, which is connected to the liquid outlet 8 through a pipeline with a pump. Since the filtrate needs to be discharged in time to ensure that the filtration device can continue to filter, a filtrate collection container 9 is provided to pump out the filtrate in time. In addition, a liquid level sensor can also be provided to monitor the liquid level in the filtrate collection container 9 to control the start and stop of the pump to ensure the filtering effect.

[0045] As an optional embodiment of the present application, the rotating mechanism 3 also includes a rotating shaft support 6, and the rotating shaft support 6 is arranged at the bottom of the filtering device; the rotating shaft support 6 is connected to the lower end of the rotating shaft 301 through a bearing 7 to support the rotating shaft 301. Since the rotating mechanism 3 needs to bear a certain weight, when the collected filter residue is large and heavy, the installation stability of its upper end is required to be higher; and when there is filter residue in only one filter cartridge, the axis of the rotating shaft 301 may be offset due to the change in the overall center of gravity, and the parts of the rotating mechanism 3 are easy to be damaged. Therefore, in order to ensure the stability of the rotating mechanism 3, a rotating shaft support 6 is arranged at the bottom, firstly, it is used for auxiliary support and force dispersion, and secondly, it can fix the axis of the rotating shaft 301 so that it does not deviate, effectively extending its service life.

[0046] As an optional embodiment of the present application, a stirring paddle 101 is provided in the reaction tank body 1. In some prior arts, since a filter plate is provided in the reaction tank body 1, it is inconvenient to provide a stirring paddle 101, and generally agitation is performed by introducing gas, which is not as effective as stirring with a stirring paddle 101 in mixing the materials. However, the present application completely separates the filtering device from the reaction tank body 1, so a stirring paddle 101 can be provided in the reaction tank body 1, which can effectively improve the efficiency of the cracking reaction.

[0047] As an optional embodiment of the present application, the cross-section of the filter barrel 4 is fan-shaped, and the size of the central angle of the fan is preferably 60°-90°. The arc edge of the filter barrel 4 matches the cylindrical shell of the filter device. This design can ensure that the volume (capacity) of the filter barrel 4 is large to the greatest extent and can fully accommodate the filter residue.

[0048] As an optional embodiment of the present application, there is a slag removal opening below the sealing cover 5, and the diameter of the slag removal opening is larger than the diameter of the inner barrel, that is, the size of the slag removal opening can allow the inner barrel to pass through; a handle can also be provided on the inner barrel, and the height of the handle is close to the height of the slag removal opening, so that the staff can take out the inner barrel easily.

[0049] As an optional implementation of the present application, see Figure 2 The side of the mounting frame is a grid plate 401, and the bottom of the mounting frame is a grid bar 402. The gap of the mounting frame is relatively large, which is only used to accommodate the inner tank and does not need to play a filtering role. The bottom grid bar 402 is used to strengthen the rigidity of the mounting frame to prevent deformation. If the selected material has sufficient rigidity, the bottom surface may not be provided with a grid bar 402, and a hook may be provided on the upper edge of the inner tank so that it can be directly hung on the mounting frame.

[0050] As an optional embodiment of the present application, a support frame 12 is further provided below the reaction tank body 1, and the reaction tank body 1 is arranged on the support frame 12; the filtering device is arranged in the space below the support frame 12. The support frame 12 divides the space into two layers, with the reaction tank body 1 arranged on the upper layer and the filtering device arranged on the lower layer, so that the space is fully utilized through a reasonable and compact layout. And in the design of the present application, the feed inlet 201 of the filtering device is offset from the central axis of the filtering device, so the filtering device will be arranged on one side below the reaction tank body 1, and the filtrate collection container 9 can be arranged on the other side, see Figure 3 , the slag taking port of the filtering device will not be blocked by the supporting frame 12.

[0051] As an optional embodiment of the present application, the housing 2 of the filter device is provided with an air inlet 10 and a gas absorption device 11, the air inlet 10 is used to purge nitrogen or other inert gases into the filter device, and a corresponding gas storage tank can be configured; the gas absorption device 11 is used to absorb harmful gases (trifluoroacetic acid) in the filter device, and specifically, alkaline solution absorption can be used. Before the staff opens the sealing cover 5 to remove the filter residue, the air inlet 10 and the gas absorption device 11 can be opened to first empty the harmful gases in the filter device, and then the filter residue is removed to prevent the volatile harmful gases from causing harm to the staff.

[0052] Those skilled in the art will appreciate that the embodiments described herein are intended to help readers understand the principles of the present invention, and should be understood that the protection scope of the present invention is not limited to such specific statements and embodiments. Those skilled in the art can make various other specific variations and combinations based on the technical inspirations disclosed in the present invention without departing from the essence of the present invention, and these variations and combinations are still within the protection scope of the present invention.

Claims

1. A polypeptide cleavage reactor, characterized in that: It comprises a reaction tank body and a filtering device, wherein a discharge port is provided at the bottom of the reaction tank body; The filtering device comprises a housing, a rotating mechanism, a feed inlet, a liquid outlet and at least two filtering barrels; The feed inlet is arranged on the top surface of the shell, and the feed inlet is connected to the discharge port; The rotating mechanism is inserted into the filtering device from above; the filtering barrel is arranged on the rotating mechanism and can be rotated to below the feeding port through the rotating mechanism; A sealing cover is provided on the top surface of the shell.

2. A polypeptide cleavage reactor according to claim 1, characterized in that: The rotating mechanism comprises a motor arranged above the housing and a rotating shaft arranged inside the housing; The filter barrel comprises a mounting frame and an inner barrel, and the mounting frame is fixedly arranged on the rotating shaft; The inner barrel is placed in the mounting frame.

3. A polypeptide cleavage reactor according to claim 2, characterized in that: The rotating mechanism further comprises a rotating shaft support, and the rotating shaft support is arranged at the bottom of the filtering device; The rotating shaft support is connected to the lower end of the rotating shaft through a bearing.

4. A polypeptide cleavage reactor according to claim 1, characterized in that: The polypeptide cleavage reactor further comprises a filtrate collecting container, and the filtrate collecting container is connected to the liquid outlet through a pipeline with a pump.

5. A polypeptide cleavage reactor according to claim 1, characterized in that: A stirring paddle is arranged in the reaction tank.

6. A polypeptide cleavage reactor according to claim 1, characterized in that: The cross section of the filter barrel is fan-shaped.

7. A polypeptide cleavage reactor according to claim 2, characterized in that: A slag taking opening is provided below the sealing cover, and a diameter of the slag taking opening is larger than a diameter of the inner tank.

8. A polypeptide cleavage reactor according to claim 1, characterized in that: A support frame is also provided below the reaction tank body, and the reaction tank body is arranged on the support frame; The filtering device is arranged in the space below the supporting frame.

9. A polypeptide cleavage reactor according to claim 1, characterized in that: The outer shell of the filter device is provided with an air blowing port and a gas absorption device.

10. A polypeptide cleavage reactor according to claim 2, characterized in that: The side surface of the mounting frame is a grid plate, and the bottom surface of the mounting frame is a grid bar.

Images