Device for being matched with centrifugal machine to carry out gradient centrifugation on liquid

Abstract

A device used for being matched with a centrifugal machine to conduct gradient centrifugation on liquid comprises a separation cavity and a column core which is arranged in the cavity and used for sucking and sealing the separation cavity, and a separation opening for preparing PRP through a secondary centrifugation method is formed in the column core. And the closed cavity formed between the column core and the separation cavity of the device can ensure that the device does not leak in the centrifugal machine, and the container does not need to be replaced for centrifugation, so that pollution is effectively avoided.

Description

Technical Field

[0001] The utility model belongs to the field of medical equipment, and relates to a device used for matching a centrifuge to centrifuge liquid gradients, in particular to a device used for separating and extracting platelet-rich plasma. Background Art

[0002] Platelet-rich plasma (PRP) is a platelet concentrate obtained by centrifuging autologous whole blood. Nowadays, there is no unified standard for the preparation of PRP. There are two main methods for its preparation: density gradient centrifugation and plasmapheresis. Plasmapheresis is a method of using medical blood component separation equipment to separate whole blood into components such as platelet plasma and concentrated platelets. This method is mainly used for platelet collection in blood banks and clinical component transfusion. Another method is density gradient centrifugation. According to the different sedimentation coefficients of each component in the blood, PRP is separated and extracted from whole blood. According to the number of centrifugation, the preparation of PRP can be divided into one, two and three centrifugation methods. Nowadays, the two-centrifugation method is widely used. A large number of studies have shown that the two-centrifugation method has the highest quality of PRP extracted, and it is also the most widely used in clinical practice.

[0003] The specific preparation steps of PRP are as follows: (1) Draw some peripheral blood into a sterile test tube containing an anticoagulant; (2) Perform the first centrifugation. After centrifugation, the blood is divided into three layers: the upper layer is platelet-poor plasma, the main component of which is fibrinogen, etc.; the middle layer is highly concentrated platelets, and the lower layer is red blood cells. (3) Use a pipette to suck up some red blood cells from the upper layer, the middle layer, and the adjacent middle layer, and transfer them to another sterile test tube. (4) After centrifugation again, the plasma is divided into three layers: the lower layer is a small amount of red blood cells, the upper layer is platelet-poor plasma, and the layer between the two layers is platelet-rich plasma. (5) Use a pipette to discard most of the supernatant, retain an appropriate amount of serum to suspend the concentrated platelets and mix well, which is PRP. (6) Add a trace amount of thrombin and calcium chloride to activate the platelets to obtain PRP gel.

[0004] With regard to the above preparation steps, there is currently no unified equipment specifically used for preparing PRP. Most of them use an open secondary centrifugation method to prepare PRP. The PRP prepared by this method needs to be transferred in multiple containers, which increases the chances of platelet activation and contamination, and is easily contaminated by the outside world; and the platelet recovery rate is relatively low; in addition, this method does not have a special application tool, is inconvenient to use, and is easily affected by operating factors, and the preparation method is not stable enough. At present, there are also a few closed secondary centrifugation methods for preparing PRP, but there are still defects such as complicated and time-consuming operation steps for medical staff, and the inability to accurately separate and extract PRP.

[0005] For example, Chinese patent CN214077269U discloses a PRP extraction device, which collects blood into a blood collection device, centrifuges the blood collection device in the device, and then uses a speed limiting device to slowly transfer the components in the centrifuged blood in sequence, thereby achieving stable and accurate extraction of PRP; although it is a closed centrifugal extraction device, because the plasma is divided into three layers after centrifugation, and the middle layer is the platelet-rich plasma layer, the red blood cells in the lower layer must be transferred to the platelet-rich plasma layer during the transfer process, and red blood cells will inevitably be mixed in during this process, losing the essence of preparing PRP by secondary centrifugation. Chinese patent CN115475416A discloses a platelet-rich plasma preparation mechanism, which first draws whole blood into the first cavity jacket, then seals and moves the separation valve core of the mechanism upward and presses the isolation plug downward, and transfers part of the whole blood to the second cavity jacket for the first centrifugation, and the red blood cell layer at the lower layer is separated to the inner cavity of the second cavity jacket, and then the separation valve core is closed and the whole mechanism is turned upside down for secondary centrifugation. After that, the PRP is located on the bottom surface of the second cavity end cover and gradually enters the inner cavity of the separation valve core through the flow hole to achieve separation, and then the sealing gasket is inserted through a syringe with a needle to extract the PRP close to the sealing gasket. However, when the above-mentioned device is centrifuged once, the reserved space in the inner cavity of the second cavity jacket needs to be reserved based on experience. If the reserved space is too small, some red blood cells cannot be separated, affecting the secondary centrifugation.

[0006] Therefore, there is an urgent need to develop a platelet-rich plasma preparation device that can avoid the risk of replacing containers and introducing other components, and can achieve stable and accurate extraction of PRP. The entire device also needs to have a simple structure, low cost, simple and easy to use, and not prone to mistakes that lead to extraction failure and safety hazards. Of course, the device also has the function of separating other liquids that can be centrifuged and layered. Summary of the invention

[0007] In order to achieve the above-mentioned purpose without causing the above-mentioned technical defects, the present application provides a device for centrifuging liquid in a centrifuge gradient, through which red blood cells in the blood after centrifugation are sucked away in an upper suction and lower discharge manner, and the platelet-rich plasma layer in the middle layer of the tube body does not need to be transferred. The specific technical scheme is as follows:

[0008] A device for gradient centrifugation of liquid in cooperation with a centrifuge, the device comprising a separation chamber 1 and a column core 2 arranged in the separation chamber 1;

[0009] The separation chamber 1 is a columnar chamber, and a suction head 4 connected to the puncture needle is arranged at the front end of the chamber; a column core 2 for pulling and sealing is also arranged in the chamber;

[0010] The column core 2 is a column, the head end 21 of the column is adapted to the separation chamber 1 and is provided with a sealing gasket 5, and the head end 21 of the column core 2 is also provided with a separation port 3 for suction from the end, and the separation port 3 is wrapped inside the sealing gasket 5;

[0011] A sealing plug is provided at the top of the suction head 4 to seal the flow hole of the suction head 4 before centrifugation.

[0012] Furthermore, the separation port 3 includes at least one suction puncture port.

[0013] Furthermore, the puncture opening is a circular hole or a notch provided at the head end 21 .

[0014] Furthermore, the column core 2 is provided with a suction channel 22 along the length direction, and the suction channel 22 is connected to the separation port 3 provided at the head end 21 .

[0015] Furthermore, the terminal port 23 of the suction channel 22 is a Luer connector, and the sealing member 6 is threadedly connected to the Luer connector.

[0016] Furthermore, the outer wall of the separation chamber 1 is provided with scale values ​​for accurate reading.

[0017] Working principle of the utility model: A device for centrifuging liquid with a centrifuge, the device comprises a separation chamber 1 and a column core 2 arranged in the separation chamber 1, the head end 21 of the column core 2 is provided with a separation port 3 for suction, when using the device to prepare PRP, the specific operation is as follows:

[0018] First, the new device is used to connect a blood collection needle to the suction port for whole blood collection. When drawing whole blood, the column core 2 is pulled upward to draw the whole blood into the closed cavity formed by the column core 2 and the separation chamber 1. After the whole blood collection is completed, the blood collection needle is removed and the suction head 4 is sealed through the plug; then the secondary centrifugation method is used to prepare PRP.

[0019] For the first centrifugation, the device containing whole blood in the closed chamber is first placed in the centrifuge for the first centrifugation (it can be placed upright or inverted). After centrifugation, the blood is divided into three layers, the upper layer is platelet-poor plasma, the middle layer is highly concentrated platelets, and the lower layer is red blood cells. The device placed upright for centrifugation extracts red blood cells by opening the suction head 4 plug and pushing the column core 2 downward. The red blood cells in the lower layer are pushed out of the suction head 4 and then sealed again by the plug. If it is necessary to extract the platelet-poor plasma from the last time, the blood collection needle can pass through the separation port 3 set by the head end 21 of the column core 2 to puncture the sealing gasket 5 to contact the platelet-poor plasma in the upper layer and extract the upper liquid; the device placed inverted can be extracted by passing the blood collection needle through the separation port 3 set by the head end 21 of the column core 2 to puncture the sealing gasket 5 and the red blood cells in contact with the separation port 3 to extract; in the process of drawing and pushing, it is inevitable that a small amount of blood will be completely separated, so a second centrifugation is required.

[0020] For the second centrifugation, the device after the first centrifugation and extraction is placed in the centrifuge again to repeat the above operation to complete the preparation of PRP.

[0021] Compared with the prior art, the beneficial effects of the utility model are:

[0022] (1) There is no need to change containers during centrifugation, the risk of mixing with other components is low, and the extracted PRP concentration is high;

[0023] (2) The device can be centrifuged in both forward and reverse directions according to the user's habits, thereby improving applicability;

[0024] (3) The device is also suitable for separating drugs, oils and fats. The sedimentation coefficients of the components are different, and the liquid after centrifugation is layered. The excess liquid is taken out upward or downward according to the layer where the actual component to be extracted is located after centrifugation. Only the required components can be retained in the device, and contamination caused by transfer can be avoided;

[0025] (3) The entire device has a simple structure, low cost, and is easy to use, and it is not easy to make mistakes that lead to extraction failure. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is a schematic structural diagram of a device for separating and extracting platelet-rich plasma according to the utility model;

[0027] Figure 2 This is a schematic diagram of the structure of the column core 2 of Example 1 of the utility model;

[0028] Figure 3 This is a diagram showing the arrangement of puncture holes of the separation port 3 of the column core 2 of Example 1 of the utility model;

[0029] Figure 4Another structural schematic diagram of the utility model;

[0030] Figure 5 This is a schematic diagram of the structure of the column core 2 of Example 2 of the utility model

[0031] Figure 6 This is a diagram showing the arrangement of puncture holes of the separation port 3 of the column core 2 of Example 2 of the present utility model;

[0032] Figure 7 It is another structural schematic diagram of the utility model;

[0033] Figure 8 This is a schematic diagram of the structure of the column core 2 of Example 3 of the utility model

[0034] Fig. 9 Another structural schematic diagram of the utility model

[0035] Fig.10 This is a schematic diagram of the structure of the column core 2 of Example 4 of the utility model;

[0036] Fig.11 This is a schematic diagram of the overall structure of Example 4 of the utility model;

[0037] In the figure, 1-separation chamber; 2-column core; 21-head end; 22-suction channel; 23-end port; 3-separation port; 31-needle inlet; 4-suction head; 5-sealing pad; 6-sealing element 6; 7-cone;. DETAILED DESCRIPTION

[0038] In order to make the purpose, technical solution and advantages of the utility model clearer, the utility model is further described in detail below in combination with specific implementation methods and with reference to the accompanying drawings. It should be understood that these descriptions are only exemplary and are not intended to limit the scope of the utility model. In addition, in the following description, the description of well-known structures and technologies is omitted to avoid unnecessary confusion of the concept of the utility model.

[0039] Example 1: A device for separating and extracting platelet-rich plasma, such as Figure 1-4As shown, the device includes a separation chamber 1 and a column core 2 arranged in the separation chamber 1; the separation chamber 1 is a cylindrical hollow cavity, and a suction head 4 connected to the puncture needle is arranged at the front end of the separation chamber 1. After the whole blood collection is completed, the suction head 4 is sealed by a plug and then centrifuged again; the outer wall of the separation chamber 1 is provided with a scale value for accurate reading; the column core 2 is arranged in the separation chamber 1, and is a "cross"-shaped columnar body whose head end 21 is adapted to the separation chamber 1. A separation port 3 for aspirating the upper platelet-poor plasma after centrifugation is opened in the middle of the head end 21 of the column core 2, and a needle inlet 31 connected to the separation port 3 is opened on the column body, and a truncated cone 7 for preventing the suction needle from being inserted into the outside of the separation port 3 and causing the needle to be stuck is also arranged between the needle inlet 31 and the head end 21, and a sealing pad 5 is wrapped around the truncated cone 7 and the outer periphery of the head end 21 of the column core 2, and the red blood cells in the lower layer after separation can be pushed out through the suction head 4 at the front end of the separation chamber 1.

[0040] Example 2: Another embodiment of a device for centrifuging liquid in a centrifuge gradient, the device being Figure 5-6 As shown, it includes a separation chamber 1 and a column core 2 arranged in the separation chamber 1; the separation chamber 1 is consistent with Example 1, the column core 2 is arranged in the separation chamber 1, and is a "cross"-shaped columnar body whose head end 21 is adapted to the separation chamber 1, and the head end 21 of the column core 2 is provided with a separation port 3, and the separation port 3 includes four puncture ports evenly arranged at the head end 21, and the positions of the puncture ports are respectively arranged at the grooves corresponding to the "cross"-shaped column core 2, so as to facilitate direct insertion of the needle from the tail end of the separation chamber 1 through the puncture port into the sealed separation chamber 1 to extract the upper platelet-poor plasma after centrifugation, and a guide hole is also provided on the truncated cone 7 opposite to the puncture port, which is used to keep the aspiration needle vertically entering the sealed separation chamber 1 for aspiration.

[0041] Example 3: Another embodiment of a device for centrifuging liquid in a centrifuge gradient, the device being Figure 7-8 As shown, it includes a separation chamber 1 and a column core 2 arranged in the separation chamber 1; the separation port 3 at the front end of the column core 2 is a uniformly opened notch, and the notch is uniformly arranged on the truncated cone 7 installed on the column core 2, the circular surface of the truncated cone 7 installed on the column core 2 is larger than the circular surface of the head end 21 of the column core 2, and the notch is a fan-shaped opening arranged along the outer periphery of the truncated cone 7, and the outer periphery of the truncated cone 7 with the fan-shaped opening and the head end 21 of the column core 2 are wrapped with a sealing gasket 5, and the red blood cells in the lower layer after separation can be pushed out through the suction head 4 at the front end of the separation chamber 1, and the device can also be inverted, and the separated red blood cells are close to one end of the column core with the fan-shaped opening, and the long needle enters the fan-shaped opening area to puncture the sealing gasket 5 for suction.

[0042] Example 4: Another embodiment of a device for centrifuging liquid in a centrifuge gradient, the device being Figure 9-11As shown, it includes a separation chamber 1 and a column core 2 arranged in the separation chamber 1; the separation chamber 1 is a cylindrical cavity, and a suction head 4 is arranged at the front end of the cavity, and the suction head 4 is sealed by a plug after completing the whole blood collection; the column core 2 is a "cross"-shaped columnar body with a head end 21 adapted to the separation chamber 1, and a protruding separation port 3 is arranged at the head end 21 of the column core 2, and a suction channel 22 communicating with the separation port 3 is arranged in the middle part of the column core 2 along the length direction, and the end port 23 of the suction channel 22 is a Luer connector, and the sealing member 6 is threadedly connected to the Luer connector.

[0043] It should be understood that the above specific embodiments of the present invention are only used to illustrate or explain the principles of the present invention, and do not constitute a limitation on the present invention. Therefore, any modifications, equivalent substitutions, improvements, etc. made without departing from the spirit and scope of the present invention should be included in the protection scope of the present invention. In addition, the claims attached to the present invention are intended to cover all changes and modifications that fall within the scope and boundaries of the attached claims, or the equivalent forms of such scope and boundaries.

Claims

1. A device for centrifuging liquid in a gradient centrifuge, characterized in that: The device comprises a separation chamber (1) and a column core (2) arranged in the separation chamber (1); The separation chamber (1) is a columnar chamber, and a suction head (4) connected to the puncture needle is arranged at the front end of the chamber; a column core (2) for pulling and sealing is also arranged in the chamber; The column core (2) is a column-shaped body, the head end (21) of the column-shaped body is adapted to the separation chamber (1) and is provided with a sealing gasket (5), the head end (21) of the column core (2) is also provided with a separation port (3) for suction from the end, and the separation port (3) is wrapped inside the sealing gasket (5); A sealing plug is provided at the top of the suction head (4) for sealing the flow hole of the suction head (4) before centrifugation.

2. A device for gradient centrifugation of liquid in conjunction with a centrifuge according to claim 1, characterized in that: The separation port (3) comprises at least one suction puncture port.

3. A device for gradient centrifugation of liquid in conjunction with a centrifuge according to claim 2, characterized in that: The puncture opening is a circular hole or a notch arranged at the head end (21).

4. A device for gradient centrifugation of liquid in conjunction with a centrifuge according to claim 1, characterized in that: The column core (2) is provided with a suction channel (22) along the length direction, and the suction channel (22) is connected to a separation port (3) provided at the head end (21).

5. A device for gradient centrifugation of liquid in conjunction with a centrifuge according to claim 4, characterized in that: The end port (23) of the suction channel (22) is a Luer connector, and the sealing member (6) is threadedly connected to the Luer connector.

6. A device for gradient centrifugation of liquid according to claim 1, characterized in that: The outer wall of the separation chamber (1) is provided with scale values ​​for accurate reading.

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