Ready-to-use BCA protein quantitative kit

Abstract

The utility model relates to the technical field of kits, in particular to a ready-to-use BCA protein quantitative kit which comprises a kit body with an opening in the top and a kit cover which covers the opening of the kit body and is rotatably connected with the kit body through an overturning connecting piece, a first notch which is concave inwards is formed in the side wall of the kit body, and a second notch which is concave inwards is formed in the side wall of the kit body. The bottom wall of the first notch is deepened and provided with a second notch, the second notch is filled with a heat preservation medium, the peripheral side wall of the box cover is provided with a protruding sealing ring matched with the first notch, and when the box cover is closed, the sealing ring abuts against the top face of the heat preservation medium in the second notch to seal the opening of the box body. The thermal insulation medium is filled in the side wall of the kit, the sealing ring is arranged on the kit cover, and the kit body is sealed when the kit is closed, so that the interior of the kit body is effectively insulated, the BCA protein reagent is prevented from losing activity due to too fast temperature loss, and the kit is suitable for occasions such as laboratories or on-site rapid detection.

Description

Technical Field

[0001] The utility model relates to the technical field of kits, in particular to a ready-to-use BCA protein quantitative kit. Background Art

[0002] The BCA method is a method for measuring the protein content in a solution. Under alkaline conditions, protein reduces Cu2+ to Cu+ and interacts with the BCA reagent to form a purple complex. This water-soluble complex shows strong absorbance at 562nm. The absorbance is proportional to the protein concentration, so the protein concentration can be calculated based on the absorbance value.

[0003] The quantitative proportioning of BCA protein reagent is an important step required before protein separation and analysis. Protein chromatography separation, protein electrophoresis analysis, protein immunoseparation and analysis, quantification of total protein released by cell lysis, labeling of protein molecules, protein structure analysis, etc. all require reliable quantitative proportioning solutions as the basis.

[0004] The patent announcement number is "CN220885454U", and the patent name is "BCA protein quantitative reagent kit", which is a utility model patent. It discloses a reagent kit with a buffer structure and improved shock resistance of the reagent bottle. The prior art also has the following defects during use: the ready-to-use BCA protein quantitative reagent needs to be stored in a specific temperature environment to avoid inactivation of the BCA protein, and the existing reagent kit lacks a heat preservation structure and has a poor heat preservation effect.

[0005] In view of this, we proposed a ready-to-use BCA protein quantification kit to solve the existing problems. Utility Model Content

[0006] The utility model aims to provide a ready-to-use BCA protein quantification kit to solve the problems raised in the above background technology.

[0007] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a ready-to-use BCA protein quantitative test kit, comprising a box body with an opening at the top, a box cover that covers the box body opening and is rotatably connected to the box body through a flip connector, a side wall of the box body is provided with a first concave notch, a second notch is deepened on the bottom wall of the first notch, the second notch is filled with a heat preservation medium, and a protruding sealing ring that fits with the first notch is provided on the surrounding side walls of the box cover, and when the box cover is closed, the sealing ring abuts against the top surface of the heat preservation medium in the second notch to seal the box body opening.

[0008] Preferably, the second notch extends vertically downward along the side wall of the box body close to the bottom wall of the box body.

[0009] Preferably, a lock body is provided at one end of the box body away from the flip connector, and a lock tongue is provided on the opposite side of the box cover, and the lock body and the lock tongue are connected magnetically to achieve rapid locking and unlocking of the box body and the box cover.

[0010] Preferably, the lock body has a first lock core built therein, a through slot is provided above the first lock core, and a second lock core is provided at the bottom of the lock tongue and passes through the through slot and is magnetically connected to the first lock core.

[0011] Preferably, a bottle holder is provided inside the box body, a heat preservation pad is provided between the bottom of the bottle holder and the bottom wall of the box body, a sealing pad is provided between the top of the bottle holder and the box cover, and the sealing pad is fixed on the bottom wall of the box cover.

[0012] Preferably, the bottle holder is provided with a plurality of reagent grooves, and the top surface of the bottle holder is provided with finger grooves communicating with the reagent grooves.

[0013] Preferably, the flip connector includes a hinge seat whose side end is fixedly connected to the side wall of the box body, and both ends of the hinge seat are connected to the rotating seat through a rotating shaft, and the rotating seat is integrally connected to the box cover.

[0014] Preferably, the hinge seat and the rotating seat of the flip connector are provided with a damping mechanism to ensure that the box cover can rotate smoothly when opening and closing.

[0015] Preferably, the heat-insulating medium in the second notch is a phase change material.

[0016] Compared with the prior art, the utility model has the following beneficial effects: by filling the side wall of the reagent box with a heat preservation medium and arranging a sealing ring on the box cover, the box body is sealed when closed, thereby effectively keeping the inside of the box body warm, preventing the BCA protein reagent from losing activity due to excessive temperature loss, and is suitable for occasions such as laboratory or on-site rapid detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the three-dimensional structure of the utility model;

[0018] Figure 2 It is a side view structural schematic diagram of the utility model;

[0019] Figure 3 It is a schematic diagram of the closed structure of the box body and the box cover of the utility model.

[0020] In the figure: 1, box body; 11, first notch; 12, second notch; 13, insulation pad; 2, lock body; 21, first lock core; 3, bottle holder; 31, reagent tank; 32, finger groove; 4, flip connector; 41, hinged seat; 42, rotating seat; 5, box cover; 51, sealing ring; 52, sealing pad; 6, lock tongue; 61, second lock core. DETAILED DESCRIPTION

[0021] The technical solution of the utility model is further described below in conjunction with the accompanying drawings and specific embodiments.

[0022] Embodiment 1

[0023] like Figure 1 and Figure 2 As shown, a ready-to-use BCA protein quantitative test kit proposed by the utility model comprises a box body 1 with an opening on the top, a box cover 5 covering the opening of the box body 1 and rotatably connected to the box body 1 through a flip connector 4, a first concave notch 11 is provided on the side wall of the box body 1, a second notch 12 is deepened on the bottom wall of the first notch 11, and a heat preservation medium is filled in the second notch 12, and a protruding sealing ring 51 that fits with the first notch 11 is provided on the surrounding side walls of the box cover 5, and when the box cover 5 is closed, the sealing ring 51 abuts against the top surface of the heat preservation medium in the second notch 12 to seal the opening of the box body 1.

[0024] Furthermore, the second notch 12 extends vertically downward along the side wall of the box body 1 close to the bottom wall of the box body 1 .

[0025] Furthermore, a lock body 2 is provided at one end of the box body 1 away from the flip connector 4, and a lock tongue 6 is provided on the opposite side of the box cover 5. The lock body 2 and the lock tongue 6 are connected magnetically to achieve rapid locking and unlocking of the box body 1 and the box cover 5.

[0026] Furthermore, the lock body 2 has a built-in first lock core 21 , a through slot is provided above the first lock core 21 , and a second lock core 61 is provided at the bottom of the lock tongue 6 and passes through the through slot and is magnetically connected to the first lock core 21 .

[0027] Furthermore, a bottle holder 3 is provided inside the box body 1 , a heat preservation pad 13 is provided between the bottom of the bottle holder 3 and the bottom wall of the box body 1 , a sealing pad 52 is provided between the top of the bottle holder 3 and the box cover 5 , and the sealing pad 52 is fixed on the bottom wall of the box cover 5 .

[0028] Furthermore, the bottle holder 3 is provided with a plurality of reagent slots 31 for placing BCA protein reagent bottles, and the top surface of the bottle holder 3 is provided with finger slots 32 connected with the reagent slots 31 to facilitate taking and placing the reagent bottles.

[0029] Furthermore, the flip connector 4 includes a hinge seat 41 whose side end is fixedly connected to the side wall of the box body 1 , and both ends of the hinge seat 41 are connected to a rotating seat 42 via a rotating shaft, and the rotating seat 42 is integrally connected to the box cover 5 .

[0030] Furthermore, the hinge seat 41 and the rotating seat 42 of the flip connector 4 are provided with a damping mechanism to ensure that the box cover 5 can rotate smoothly when opening and closing.

[0031] Furthermore, the heat-insulating medium in the second slot 12 is a phase change material.

[0032] The working principle of the ready-to-use BCA protein quantitative kit based on the first embodiment is: during storage, a well-proportioned BCA protein reagent bottle is loaded into the kit, and according to the storage requirements of the BCA protein reagent, the kit is placed in an environment suitable for storing the BCA protein reagent, and the temperature in the box body 1 is made to reach the temperature for storing the BCA protein reagent by heating or refrigeration;

[0033] When the reagent kit leaves the suitable temperature environment when being taken out, the heat preservation medium filled in the second slot 12 on the side wall of the box body 1 absorbs or releases heat when the temperature changes, thereby maintaining the temperature stability in the box body 1. The box cover 5 is provided with a sealing ring 51. When the box cover 5 is closed, the sealing ring 51 will abut against the top surface of the heat preservation medium in the second slot 12 to form an effective seal, thereby ensuring the sealing of the opening of the box body 1 and preventing the external environment from affecting the BCA protein reagent in the box body 1, thereby preserving the BCA protein reagent during use and avoiding the inactivation of the BCA protein reagent.

[0034] The above-mentioned specific embodiments are only several preferred embodiments of the present invention. Based on the technical solution of the present invention and the relevant inspiration of the above-mentioned embodiments, those skilled in the art can make various alternative improvements and combinations to the above-mentioned specific embodiments.

Claims

1. A ready-to-use BCA protein quantification kit, comprising a box body (1) having an opening at the top, a box cover (5) covering the opening of the box body (1) and rotatably connected to the box body (1) via a flip connector (4), characterized in that: The side wall of the box body (1) is provided with a first concave notch (11), the bottom wall of the first notch (11) is further provided with a second notch (12), the second notch (12) is filled with a heat-insulating medium, and the surrounding side walls of the box cover (5) are provided with a protruding sealing ring (51) that fits with the first notch (11); when the box cover (5) is closed, the sealing ring (51) abuts against the top surface of the heat-insulating medium in the second notch (12), thereby sealing the opening of the box body (1).

2. A ready-to-use BCA protein quantification kit according to claim 1, characterized in that: The second notch (12) extends vertically downward along the side wall of the box body (1) close to the bottom wall of the box body (1).

3. A ready-to-use BCA protein quantification kit according to claim 1, characterized in that: A lock body (2) is provided at one end of the box body (1) away from the flip connector (4), and a lock tongue (6) is provided on the opposite side of the box cover (5); the lock body (2) and the lock tongue (6) are connected magnetically to achieve rapid locking and unlocking of the box body (1) and the box cover (5).

4. A ready-to-use BCA protein quantification kit according to claim 3, characterized in that: The lock body (2) has a first lock core (21) built in, a through slot is provided above the first lock core (21), and a second lock core (61) is provided at the bottom of the lock tongue (6) and passes through the through slot and is magnetically connected to the first lock core (21).

5. A ready-to-use BCA protein quantification kit according to claim 1, characterized in that: A bottle holder (3) is provided inside the box body (1), a heat preservation pad (13) is provided between the bottom of the bottle holder (3) and the bottom wall of the box body (1), a sealing pad (52) is provided between the top of the bottle holder (3) and the box cover (5), and the sealing pad (52) is fixed on the bottom wall of the box cover (5).

6. A ready-to-use BCA protein quantification kit according to claim 5, characterized in that: The bottle holder (3) is provided with a plurality of reagent grooves (31), and the top surface of the bottle holder (3) is provided with finger grooves (32) which are in communication with the reagent grooves (31).

7. A ready-to-use BCA protein quantification kit according to claim 1, characterized in that: The flip connection member (4) comprises a hinge seat (41) whose side end is fixedly connected to the side wall of the box body (1); both ends of the hinge seat (41) are connected to a rotating seat (42) via a rotating shaft; and the rotating seat (42) is integrally connected to the box cover (5).

8. A ready-to-use BCA protein quantification kit according to claim 7, characterized in that: The hinge seat (41) and the rotating seat (42) of the flip connector (4) are provided with a damping mechanism to ensure that the box cover (5) can rotate smoothly when opening and closing.

9. A ready-to-use BCA protein quantification kit according to claim 1, characterized in that: The heat-insulating medium in the second notch (12) is a phase-change material.

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