Cryopreservation device of semen cryopreservation tube

Abstract

The utility model provides a cryopreservation device of a seminal fluid cryopreservation tube, which comprises a freezing box used for containing liquid nitrogen; the box cover is used for sealing the opening of the freezing box; the automatic overturning component is arranged in the freezing box and comprises two supports which are oppositely arranged left and right, inclined frames are formed on the supports respectively, and the inclined frames on the left side and the right side define a containing groove used for containing a freezing tube; rollers are respectively arranged on the inclined frames, and the rolling surfaces of the rollers protrude into the placing grooves so as to be in contact with the outer walls of the cryopreservation tubes in the placing grooves; the rollers are rotatably arranged and connected with a driving device, and the rotation directions of the rollers are the same so as to synchronously drive the cryopreservation tube to turn over during rotation, so that the cryopreservation tube can be continuously turned over by 360 degrees in all directions, liquid nitrogen steam evaporated in the freezing box can uniformly fumigate each surface of the semen cryopreservation tube, and the semen cryopreservation effect is improved. The semen in the cryopreservation tube is precooled more uniformly, the precooling effect is better, and the cryopreservation effect of the semen is more ideal.

Description

Technical Field

[0001] The utility model relates to the technical field of assisted reproductive equipment, in particular to a freezing device for semen cryopreservation tubes. Background Technique

[0002] Semen cryopreservation is of great significance. For example, during the implementation of assisted reproductive technology (ART) treatment, there are patients with difficulties in sperm collection. If the female has obtained eggs on the day of egg retrieval, but the male cannot obtain sperm, it will be difficult to carry out in vitro fertilization normally; for such patients, pre-freezing semen as a backup can reduce the sperm collection pressure on the male on the day of egg retrieval. Another example is that some male patients need to receive radiotherapy and chemotherapy due to malignant tumors, and radiotherapy and chemotherapy may seriously affect the quantity and quality of sperm. Then, cryopreserving semen before cancer treatment to preserve male fertility will be a beneficial reproductive insurance measure. Or, for patients with oligospermia or asthenospermia, the method of ejaculating semen in fractions, cryopreserving it, and then thawing and mixing it uniformly can be used for ART treatment, which can enrich and obtain more sperm, thereby increasing the success rate of in vitro fertilization. In addition, sperm obtained during surgery (testicular tissue biopsy or percutaneous epididymal sperm aspiration) is also worthy of cryopreservation, so as to avoid the need for repeated surgery to obtain sperm during the treatment cycle or prevent the failure of sperm collection next time.

[0003] In the prior art, there are mainly three ways of semen freezing: programmed freezing, liquid nitrogen vapor fumigation freezing, and vitrification freezing. Among them, programmed freezing requires the purchase of expensive programmed freezer, and the whole process is relatively slow, and the freezing effect is not significantly improved compared with the other two methods, so it is currently less commonly used; vitrification freezing is achieved by directly inserting the semen sample and cryoprotectant mixture into liquid nitrogen, which can be applied to the freezing of rare sperm (less liquid volume, not easy to crystallize), but for the freezing of large-volume cryopreservation tubes, ice crystals often occur, resulting in poor freezing effect; liquid nitrogen vapor fumigation freezing is a method of pre-cooling the semen sample with the low temperature provided by the nitrogen gas generated by the evaporation of liquid nitrogen and then putting it into liquid nitrogen, which can help sperm quickly cross the dangerous period of ice crystal formation. This method is simple, time-saving and is adopted by more reproductive centers.

[0004] In the prior art, liquid nitrogen vapor fumigation and freezing often adopt the method of clamping the cryotube containing semen and cryoprotectant at the bayonet of the aluminum rack, and placing the aluminum rack above the liquid nitrogen fumigation box for fumigation. This method is simple and easy to implement, but there are also some deficiencies. For example, the liquid nitrogen vapor only fumigates the downward-facing side of the cryotube, and the upward-facing side of the cryotube cannot be effectively fumigated, resulting in insufficient and uneven fumigation of the whole, affecting the precooling effect; the fixing method of clamping the tube by the aluminum rack has the risk of insecure buckling, and there is a hidden danger that the semen cryotube may fall into the liquid nitrogen due to loosening at the tube-clamping position before sufficient precooling; as the liquid nitrogen volatilizes, the liquid nitrogen level will drop accordingly, thus increasing the distance from the freezing tube, reducing the cooling rate and weakening the cooling effect. It is very difficult to make the cryotube synchronously descend with the liquid nitrogen level drop in the conventional aluminum rack tube-clamping method. Often, it can only be remedied by manually lowering the height of the aluminum rack by hand or adding liquid nitrogen into the box continuously. Holding the aluminum rack easily causes the liquid nitrogen surface to boil and the temperature to fluctuate greatly due to the large temperature difference between the hand and the liquid nitrogen, and the method of adding liquid nitrogen will increase the consumption of liquid nitrogen, and the amount of pouring must be carefully controlled during the process.

[0005] In view of the above deficiencies, Chinese Patent CN219249026U and US Patent US12010998B1 both disclose a semen cryopreservation device, which can realize the turning of the cryotube by driving the cryotube to rotate through the inclination of the tray, so that the liquid nitrogen vapor can fumigate the cryotube more evenly and improve the freezing effect. However, this fumigation and precooling method is a step-by-step method, that is, in the first half, the liquid nitrogen fumigates one side of the cryotube all the time, and in the second half, the liquid nitrogen fumigates the other side of the cryotube after rolling and turning 180°. Such a method has greatly improved compared with the prior art (only fumigating one side), but still cannot maintain continuous and uniform fumigation of the cryotube throughout the whole process in all directions, affecting the precooling effect.

[0006] Therefore, the above prior art has at least the following technical problems: The method of using liquid nitrogen vapor to fumigate the semen cryotube in the prior art cannot maintain continuous and uniform fumigation of the cryotube throughout the whole process in all directions, affecting the precooling effect. Utility Model Content

[0007] By providing a cryopreservation device for semen cryotubes in an embodiment of the present application, the technical problem that the method of using liquid nitrogen vapor to fumigate semen cryotubes in the prior art cannot maintain continuous and uniform fumigation of the cryotubes throughout the whole process in all directions and affects the precooling effect is solved.

[0008] To solve the above technical problems, an embodiment of the present application provides a cryopreservation device for semen cryotubes, and the cryopreservation device includes:

[0009] A freezing box, which can be used to hold liquid nitrogen;

[0010] A box cover, which is used to seal the opening of the freezing box;

[0011] An automatic flipping component, which is arranged in the freezing box, and the automatic flipping component includes two brackets arranged oppositely left and right. Oblique frames are respectively formed on the brackets, and the oblique frames on the left and right sides enclose to form a placement groove for placing cryopreservation tubes;

[0012] Rollers are respectively arranged on the oblique frames, and the rolling surfaces of the rollers protrude into the placement groove to contact the outer walls of the cryopreservation tubes located in the placement groove; the rollers are rotatably arranged and connected with a driving device, and the rotation directions of the rollers are the same, so as to drive the cryopreservation tubes to flip when rotating.

[0013] Furthermore, the oblique frames gradually incline and approach the other bracket on the opposite side from top to bottom, so the distance between the left and right oblique frames gradually decreases from top to bottom, and the formed placement groove gradually decreases from top to bottom.

[0014] Furthermore, the bracket includes a bottom plate for being arranged on the bottom surface of the freezing box, and half frames arranged oppositely and spaced apart in the front-rear direction on the bottom plate, and each half frame respectively includes:

[0015] A main rod, with the bottom end fixed on the bottom plate and the top end extending upward;

[0016] An inclined rod, with one end fixed to the top end of the main rod and the other end extending upward and obliquely outward;

[0017] Wherein, the inclined rods on the two half frames form the oblique frames, the rotating shaft of the roller is arranged between the two inclined rods, and one end of the rotating shaft is rotatably penetrated through one of the inclined rods, and the other end is coaxially connected with the output shaft of the first motor.

[0018] Furthermore, the half frame further includes:

[0019] An inclined strut, with one end fixedly connected to the main rod and the other end extending obliquely toward the bottom plate and fixed on the bottom plate;

[0020] A fixing rod, fixed on the bottom plate, and one end of the fixing rod is connected to the inclined strut, and the other end is fixed to the main rod;

[0021] Wherein, the inclined strut, the fixing rod and the main rod form a triangle.

[0022] Furthermore, the main rod of at least one side of the bracket is gradually obliquely arranged outward from top to bottom.

[0023] Furthermore, at least two rollers are arranged on each oblique frame from top to bottom, and the rollers on the two oblique frames are arranged in one-to-one correspondence left and right;

[0024] At least one of the brackets can move left and right in the horizontal direction to approach or move away from the other bracket, so as to guide the cryotube to move downward synchronously when rotating between the two inclined frames.

[0025] Furthermore, the bracket is connected to a second motor through a traction rope. When the second motor rotates, the traction rope winds around the output shaft of the second motor, thereby driving the bracket to move.

[0026] Furthermore, the second motor is connected to a controller, and the controller controls the moving distance of the bracket by controlling the second motor.

[0027] Furthermore, an anti-slip structure is provided on the rotating surface of the roller;

[0028] The anti-slip structure is an anti-slip strip provided on the rotating surface, and the anti-slip strip extends along the axial direction of the rotating surface and is arranged at intervals along the circumferential direction of the rotating surface in multiple numbers.

[0029] Furthermore, a liquid nitrogen level mark is provided on the bracket, and the liquid nitrogen level mark is used to indicate the level of liquid nitrogen.

[0030] One or more technical solutions provided in the embodiments of the present application have at least the following technical effects or advantages:

[0031] (1) By providing the placement groove to accommodate the cryotube, the cryotube can be pre-cooled by the liquid nitrogen vapor fumigation in the freezing box. Rollers for driving the cryotube to flip are provided on both sides of the placement groove. By driving the rollers to rotate, the cryotube can be synchronously driven to flip, and the cryotube can be continuously flipped 360° in all directions. Thus, the liquid nitrogen vapor evaporated from the freezing box can evenly fumigate each surface of the semen cryotube, and the semen in the cryotube is pre-cooled more evenly, with a better pre-cooling effect. Furthermore, the semen cryopreservation effect is more ideal, effectively solving the technical problem in the prior art that the method of using liquid nitrogen vapor to fumigate the semen cryotube cannot maintain continuous and uniform fumigation of the cryotube throughout the whole process in all directions, affecting the pre-cooling effect.

[0032] (2) The placement groove is in a "funnel shape" with a larger upper part and a smaller lower part. In this way, the cryotube is placed more stably, avoiding accidental dropping of the cryotube.

[0033] (3) By gradually increasing the distance between the two brackets, the cryogenic vial can move downward along the "funnel" while "rotating". When the liquid level of liquid nitrogen drops, the distance between the two brackets can be increased by moving the brackets, so that the cryogenic vial moves downward in the placement groove, so that the distance between the cryogenic vial and the liquid surface of liquid nitrogen remains consistent, avoiding the reduction of the cooling rate and the weakening of the cooling effect caused by the volatilization of liquid nitrogen; in addition, when the cryogenic vial is sufficiently pre-cooled in the placement groove, the brackets can be directly moved to make the distance between the two brackets large enough to allow the cryogenic vial to directly fall from the placement groove into the liquid nitrogen in the freezing box for freezing, which is very convenient.

[0034] (4) By controlling the first motor and the second motor through the controller, the moving distance of the bracket can be automatically adjusted, and the automatic freezing of the semen cryogenic vial can be completed with one key, eliminating steps such as personnel staying, timing, and manual control, and having a higher degree of automation.

[0035] (5) The diagonal strut, the fixed rod and the main rod form a triangle, so that the half-frame can be stably fixed on the bottom plate. The setting of the bottom "triangle" support structure and the bottom plate for bottom load-bearing both increase the overall stability of the device and avoid the occurrence of the device tipping over;

[0036] (6) The entire bracket is formed by using slender rod-like structures except for the bottom plate, rather than panel-like structures or block-like structures. In this way, the liquid nitrogen vapor can better pass upward through the bracket to reach the cryogenic vial, avoiding excessive unnecessary negative interference caused by the bracket itself to the upward evaporation of the liquid nitrogen vapor.

[0037] (7) When the two main rods of the right bracket tilt downward and to the right at a certain angle, it can effectively prevent the cryogenic vial from getting stuck between the left and right brackets due to frosting on the outer surface during the freezing process, and ensure that the cryogenic vial can achieve free fall after leaving the narrowest part of the bracket.

[0038] (8) The rotating surface of the roller is provided with an anti-slip structure, which can increase the friction between the rotating surface and the cryogenic vial, avoid the cryogenic vial from falling when turning over, and enable the cryogenic vial to better complete "rotation".

[0039] (9) The freezing box is made of rigid foam, which can effectively insulate heat to reduce the heat transfer between the freezing box and the outside world. The inner wall of the freezing box is provided with an integrally formed stainless steel inner liner, and a low thermal conductivity material is coated on the stainless steel inner liner to further reduce the heat transfer between the freezing box and the outside world, playing a role in preventing the rapid loss of liquid nitrogen. In addition, the box cover is made of transparent glass material, which is convenient for viewing inside and plays a role in preventing the loss of liquid nitrogen from the opening of the freezing box. Description of the Drawings

[0040] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0041] Figure 1 It is a schematic diagram of the overall structure of a freezing device for a semen freezing tube in one embodiment of the utility model;

[0042] Figure 2 This is a front view of an automatic flipping component in one embodiment of the utility model;

[0043] Figure 3 This is a schematic diagram of the structure of the automatic flipping component in one embodiment of the utility model. Figure 1 ;

[0044] Figure 4 This is a schematic diagram of the structure of the automatic flipping component in one embodiment of the utility model. Figure 2 ;

[0045] Figure 5 It is a schematic diagram of the local structure of the roller in one embodiment of the utility model;

[0046] Figure 6 This is a schematic diagram of the working state of a cryopreservation device for a sperm cryopreservation tube in an embodiment of the utility model. Figure 1 (The cryotube is placed in the storage tank);

[0047] Figure 7 This is a schematic diagram of the working state of a cryopreservation device for a sperm cryopreservation tube in an embodiment of the utility model. Figure 2 (When the cryotube is about to be removed from the placement tank);

[0048] Figure 8 This is a schematic diagram of the working state of a cryopreservation device for a sperm cryopreservation tube in an embodiment of the utility model. Figure 3 (The cryovial is dropped into liquid nitrogen). DETAILED DESCRIPTION

[0049] The embodiment of the present application provides a cryopreservation device for semen cryopreservation tubes, thereby solving the technical problem that the method of fumigating semen cryopreservation tubes with liquid nitrogen vapor in the prior art cannot maintain continuous and uniform fumigation of the cryopreservation tubes in all directions throughout the entire process, thus affecting the pre-cooling effect.

[0050] In order to better understand the above technical solution, the above technical solution will be described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0051] In one or more embodiments of the present application, a freezing device for a semen cryopreservation tube is provided, which can drive the continuous 360° omnidirectional flipping of the cryopreservation tube 9, so that the liquid nitrogen vapor can evenly fumigate each surface of the semen cryopreservation tube 9, realizing sufficient pre-cooling of the semen sample.

[0052] As Figures 1 to 8 shown, a freezing device for a semen cryopreservation tube according to an embodiment of the present application, the freezing device includes:

[0053] A freezing box 1, which can be used to hold liquid nitrogen;

[0054] A box cover 2, which is used to seal the opening of the freezing box 1;

[0055] An automatic flipping component, which is arranged in the freezing box 1, and the automatic flipping component includes two brackets 3 arranged oppositely left and right in the freezing box 1. Oblique frames are respectively formed on the brackets 3, and the oblique frames on the left and right sides enclose to form a placement groove for placing the cryopreservation tube 9;

[0056] Rollers 4 are respectively arranged on the oblique frames, and the rolling surfaces of the rollers 4 protrude into the placement groove to contact the outer wall of the cryopreservation tube 9 located in the placement groove. The rollers 4 are rotatably arranged and connected with a driving device, and the rotation directions of the rollers 4 are the same, so as to drive the cryopreservation tube 9 to flip when rotating.

[0057] It can be known from the above description that the freezing device for the semen cryopreservation tube according to the embodiment of the present application accommodates the cryopreservation tube 9 by setting the placement groove, so that the liquid nitrogen vapor in the freezing box 1 fumigates and pre-cools the cryopreservation tube 9, and rollers 4 for driving the cryopreservation tube 9 to flip are arranged on both sides of the placement groove. By driving the rollers 4 to rotate, the cryopreservation tube 9 can be synchronously driven to flip, and the continuous 360° omnidirectional flipping of the cryopreservation tube 9 can be realized, so that the liquid nitrogen vapor evaporated in the freezing box can evenly fumigate each surface of the semen cryopreservation tube 9. Then, the semen in the cryopreservation tube 9 is pre-cooled more evenly, the pre-cooling effect is better, and further the semen cryopreservation effect is more ideal, effectively solving the technical problem that the method of using liquid nitrogen vapor to fumigate the semen cryopreservation tube 9 in the prior art cannot maintain the whole-process, omnidirectional and continuous uniform fumigation of the cryopreservation tube 9, affecting the pre-cooling effect.

[0058] Specifically, in the embodiment of the present application, the freezing box 1 is in the shape of a hollow cuboid, and the material of the freezing box 1 is rigid foam, such as polyurethane (PU) or polyethylene (PE). Due to its porous structure, it can effectively insulate heat to reduce the heat transfer between the freezing box 1 and the outside; and the density of the foam material is relatively low, making it light in weight and convenient to move.

[0059] In addition, an integrally formed stainless steel inner liner is provided on the inner wall of the freezing box 1, and a low thermal conductivity material (such as polyurethane) is coated on the stainless steel inner liner to further reduce the heat transfer between the freezing box 1 and the outside world, thereby preventing the rapid loss of liquid nitrogen.

[0060] In addition, an opening is provided on the top surface of the freezing box 1 to facilitate the addition of liquid nitrogen, the placement or removal of the cryopreservation tube 9, etc. The box cover 2 can be fastened to the opening of the freezing box 1, and the box cover 2 is made of transparent glass material to facilitate viewing inside and prevent the loss of liquid nitrogen from the opening of the freezing box 1.

[0061] In an embodiment of the present application, as Figures 1 to 4 or Figures 6 to 8 shown, the diagonal frame gradually inclines and approaches the opposite bracket 3 from top to bottom, so that the distance between the left and right diagonal frames gradually decreases from top to bottom, making the placement groove in the shape of a "funnel" with a larger top and a smaller bottom. In this way, placing the cryopreservation tube 9 is more stable and prevents the cryopreservation tube 9 from accidentally falling.

[0062] In an embodiment of the present application, as Figures 1 to 4 or Figures 6 to 8 shown, the roller 4 is coaxially and fixedly connected to the output shaft of the first motor 5 to drive the roller 4 to rotate.

[0063] In an embodiment of the present application, as Figures 1 to 4 or Figures 6 to 8 shown, at least two rollers 4 are provided on each diagonal frame from top to bottom, and the rollers 4 on the two diagonal frames are arranged in a one-to-one left-right correspondence, and the diameters of all the rollers 4 are the same;

[0064] At least one of the brackets 3 can move left and right in the horizontal direction to approach or move away from the other bracket 3, so as to guide the cryopreservation tube 9 to move downward synchronously when rotating between the two diagonal frames.

[0065] Specifically, for example, the left bracket 3 is fixed in the freezing box 1, and the right bracket 3 can move left and right in the horizontal direction. In this way, the two brackets 3 approach or move away from each other.

[0066] It can be understood that by gradually increasing the distance between the two brackets 3, the cryopreservation tube 9 can move downward along the "funnel" while "rotating". When the liquid nitrogen level drops, the distance between the two brackets 3 can be increased by moving the bracket 3, so that the cryopreservation tube 9 moves downward in the placement groove, as Figure 6 、 7 shown, so as to keep the distance between the cryopreservation tube 9 and the liquid nitrogen surface consistent, avoiding the reduction of the cooling rate and the weakening of the cooling effect caused by the volatilization of liquid nitrogen.

[0067] In addition, when the cryotube 9 is sufficiently pre-cooled in the placement groove, the bracket 3 can be directly moved to increase the distance between the two brackets 3 to a size that allows the cryotube 9 to directly drop from the placement groove into the liquid nitrogen in the freezing box 1 for freezing, as Figure 8 shown, which is very convenient.

[0068] In the prior art, an operator needs to stay beside the device and time the completion of corresponding step operations, and manually pull out multiple limit plates at specific time nodes to adjust the turning of the cryotube, which is inconvenient to use and has low work efficiency.

[0069] For this reason, in an embodiment of the present application, as Figure 1 、 6 ~8 shown, the bracket 3 on the right side is connected to a second motor 6 through a traction rope 7. When the second motor 6 rotates, the traction rope 7 is wound around the output shaft of the second motor 6, thereby driving the bracket 3 on the right side to move to the right.

[0070] Furthermore, the first motor 5 and the second motor 6 are connected to a controller 8, and the controller 8 controls the start / stop, rotation speed, and rotation direction of the first motor 5 and the second motor 6.

[0071] Therefore, by controlling the first motor 5 and the second motor 6 through the controller 8 and autonomously adjusting the moving distance of the bracket 3, the automatic freezing of the semen cryotube 9 can be completed in one key (the cryotube 9 rotates while moving downward at a constant speed until it breaks away from the narrowest part of the placement groove and falls into the liquid nitrogen), eliminating steps such as personnel staying, timing, and manual control, and having a higher degree of automation.

[0072] The specific control methods of the first motor 5 and the second motor 6, such as speed, direction, and rotation time, can be set according to specific needs to control the movement of the roller 4 and the bracket 3 respectively.

[0073] Furthermore, as Figure 1 、 Figures 6 to 8 shown, the second motor 6 is arranged on the outer wall of the freezing box 1 to avoid the influence of liquid nitrogen volatilization on the second motor 6, and the traction rope 7 hermetically passes through the through hole 11 on the wall of the freezing box 1 and is connected to the output shaft of the second motor 6.

[0074] Specifically, the towing rope 7 is a coiled chain. To ensure the stability of the right bracket 3 during towing, there are two chains. One end of each chain is fixedly connected to the two main rods 32 (see details below) of the right bracket 3, and the other end is wound around the output shaft of the second motor 6. When the second motor 6 rotates, the chains are wound around the output shaft of the second motor 6, thereby jointly towing and pulling the right bracket 3 to move rightward and away from the left bracket 3, enabling the cryotube 9 to move downward along the "funnel" while "rotating".

[0075] Furthermore, the chains are connected to the middle upper section of the main rod 32 of the right bracket 3, taking into account the overall center of gravity of the bracket 3 and ensuring that it is not too close to the liquid nitrogen surface or even immersed in the liquid nitrogen, so as to enable the smooth progress of the uniform pulling process. In addition, since the distance that the right bracket 3 needs to move is not large (not exceeding the diameter of the cryotube), the requirement for the towing speed is not high, which also provides conditions for the second motor 6 to smoothly pull the right bracket 3.

[0076] In an embodiment of the present application, as Figures 1 to 4 、 Figures 6 to 8 shown, the bracket 3 includes bottom plates 331, 332 for being arranged on the bottom surface of the freezing box 1, and half frames 33 arranged at intervals in the front-rear direction on the bottom plates 331, 332. Each half frame 33 respectively includes:

[0077] Main rods 31, 32, with the bottom ends fixed on the bottom plates 331, 332 and the top ends extending upward;

[0078] Diagonal rods 311, 321, with one end fixed to the top ends of the main rods 31, 32 and the other end extending upward and outward;

[0079] Diagonal braces 312, 322, with one end fixedly connected to the middle of the main rods 31, 32 and the other end extending obliquely toward the bottom plates 331, 332 and fixed on the bottom plates 331, 332;

[0080] Fixed rods, fixed on the bottom plates 331, 332, with one end of the fixed rods connected to the diagonal braces 312, 322 and the other end fixed to the main rods 31, 32;

[0081] Among them: the diagonal braces 312, 322, the fixed rods and the main rods 31, 32 form a triangle, enabling the half frame 33 to be stably fixed on the bottom plates 331, 332. The setting of the bottom "triangle" support structure and the bottom plates 331, 332 for bottom load-bearing both increase the overall stability of the device and prevent the device from tipping over;

[0082] It can be understood that the entire bracket 3 is formed by an elongated rod-shaped structure except for the bottom plates 331 and 332, rather than a panel-like structure or a block structure. In this way, the liquid nitrogen vapor can better pass upward through the bracket 3 to reach the cryogenic tube 9, avoiding excessive unnecessary negative interference caused by the bracket 3 itself to the upward evaporation of the liquid nitrogen vapor.

[0083] In addition, the entire bracket 3 can be made of stainless steel to adapt to the liquid nitrogen environment and have sufficient strength.

[0084] It can be understood that in the embodiments of the present application, taking the middle of the two brackets 3 as the inside and the outside of the two brackets 3 respectively as the outside. It should be noted that in the embodiments of the present application, if terms indicating orientation or positional relationship such as "top", "bottom", "upper", "lower", "left", "right", "inside", "outside", etc. appear, they are based on the orientation or positional relationship shown in the drawings, only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, so it cannot be understood as a limitation to the present application.

[0085] Furthermore, as Figures 1 to 4 、 Figures 6 to 8 shown, the diagonal rods 311 and 321 on the two half brackets 33 form the diagonal bracket, the rotating shaft of the roller 4 is arranged between the two diagonal rods 311 and 321, and one end of the rotating shaft is rotatably inserted into one of the diagonal rods 311 and 321, and the other end is coaxially connected to the output shaft of the first motor 5. Among them, the first motor 5 is fixed on the diagonal rods 311 and 321, and the first motor 5 can be provided with a corresponding protective cover to isolate the liquid nitrogen and reduce the influence on the first motor 5.

[0086] Even further, as Figures 1 to 4 、 Figures 6 to 8 shown, the main rod 31 of the bracket 3 on the left side is vertically arranged with the bottom plate 331, and the bottom end of the main rod 32 of the bracket 3 on the right side is inclined to the right and fixed on the bottom plate 332, forming a "K"-shaped bracket 3.

[0087] It can be understood that if the two main rods 32 of the right bracket 32 are inclined downward and to the right at a certain angle, it can effectively prevent the cryogenic tube 9 from getting stuck between the left and right brackets 3 due to frosting on the outer surface during the freezing process, and can ensure that the cryogenic tube 9 realizes free fall after leaving the narrowest part of the "K"-shaped bracket 3.

[0088] In an embodiment of the present application, as Figure 5As shown, an anti-slip structure 41 is provided on the rotating surface of the roller 4. For example, the anti-slip structure 41 can be anti-slip strips provided on the rotating surface, and the anti-slip strips extend along the axial direction of the rotating surface and are arranged at intervals along the circumferential direction of the rotating surface in multiple numbers.

[0089] It can be understood that setting the anti-slip strips can increase the friction between the rotating surface and the cryopreservation tube 9, prevent the cryopreservation tube 9 from falling when being turned over, and enable the cryopreservation tube 9 to better complete "self-rotation".

[0090] Furthermore, the rolling surface can be made of silver metal. Using silver as the material, its temperature conduction effect is better than that of other materials such as aluminum or stainless steel.

[0091] In the embodiment of the present application, as Figures 1 to 4 、 Figures 6 to 8 shown, a liquid nitrogen level mark 34 is provided on the bracket 3, and the liquid nitrogen level mark 34 is used to indicate the liquid level of liquid nitrogen.

[0092] Specifically, the setting of the liquid nitrogen level mark 34 can more precisely control the distance between the liquid nitrogen and the cryopreservation tube 9.

[0093] Exemplarily, the usage process of the cryopreservation device in the embodiment of the present application is as follows:

[0094] When in use, first manually align and close the left bracket 31 and the right bracket 32, add liquid nitrogen to the freezing box 1, and make the liquid surface exactly at the position of the liquid nitrogen level mark 34; at this time, the distance between the liquid nitrogen surface and the narrowest part of the right bracket 3 is 4 cm;

[0095] Start the first motor 5 to make all the silver rollers 4 rotate at a uniform speed of 10 seconds / circle;

[0096] As Figure 6 shown, place the cryopreservation tube 9 naturally above the center of the silver drum 4. In the state where the left bracket 31 and the right bracket 32 are aligned and closed, the height of the bottom surface of the cryopreservation tube 9 from the liquid nitrogen surface is exactly 5 cm;

[0097] Cover the box cover 2, turn on the switch 8 of the second motor 6, and let the second motor 6 drive the traction rope 7 to traction the right bracket 32 to move to the right at a uniform speed. Without any operation, after 10 minutes, the traction rope 7 traction the right bracket 32 automatically moves to the right and opens about 1 cm. At this time, the cryopreservation tube 9 moves downward to the narrowest part of the bracket 3 while "self-rotating", as Figure 7 shown, and as the liquid nitrogen volatilizes, the height of the bottom surface of the cryopreservation tube 9 from the liquid nitrogen surface is still 5 cm;

[0098] As the right bracket 32 continues to move to the right, the cryopreservation tube 9 freely falls into the liquid nitrogen, as Figure 8As shown, the entire semen sample freezing process is completed.

[0099] It should be understood that although the quantitative terms "first", "second", etc. may be used herein to describe various units, these units should not be limited by these terms. These terms are only used to distinguish one unit from another. For example, without departing from the scope of the exemplary embodiments, the first unit may be referred to as the second unit, and similarly the second unit may be referred to as the first unit.

[0100] The outer, middle, inner and other orientation terms mentioned or possibly mentioned in this specification are defined relative to the structures shown in the respective drawings. They are relative concepts and may therefore change accordingly depending on their different positions and usage states. Therefore, these or other orientation terms should not be construed as restrictive terms.

[0101] The above are only the preferred embodiments of the present application, and do not impose any formal or substantial limitations on the present application. It should be noted that for those of ordinary skill in the art in this technical field, without departing from the method of the present application, several improvements and supplements can still be made, and these improvements and supplements should also be regarded as within the protection scope of the present utility model. For those skilled in the art, without departing from the spirit and scope of the present application, any equivalent changes made by making some modifications, decorations and evolutions using the technical content disclosed above are equivalent embodiments of the present application; at the same time, any equivalent changes made by making modifications, decorations and evolutions to the above embodiments based on the substantial technology of the present application still fall within the scope of the technical solution of the present application.

Claims

1. A cryopreservation device for semen cryopreservation tubes, characterized in that, The cryopreservation device includes: A freezing box, which can be used to hold liquid nitrogen; A box cover, which is used to seal the opening of the freezing box; An automatic flipping component, which is arranged in the freezing box, and the automatic flipping component includes two brackets arranged opposite to each other left and right. Oblique frames are respectively formed on the brackets, and the oblique frames on the left and right sides enclose a placement groove for placing cryopreservation tubes; Rollers are respectively arranged on the oblique frames, and the rolling surfaces of the rollers protrude into the placement groove to contact the outer walls of the cryopreservation tubes located in the placement groove; The rollers are rotatably arranged and connected to a driving device, and the rotation directions of the rollers are the same, so as to drive the cryopreservation tubes to flip when rotating.

2. The cryopreservation device for a semen cryopreservation tube according to claim 1, wherein, The oblique frames gradually incline and approach the other bracket on the opposite side from top to bottom, so the distance between the left and right oblique frames gradually decreases from top to bottom, and the formed placement groove gradually decreases from top to bottom.

3. The cryopreservation device for a semen cryopreservation tube according to claim 1, characterized in that, The bracket includes a bottom plate for being arranged on the bottom surface of the freezing box, and half frames arranged at intervals relative to each other in the front-rear direction on the bottom plate, and each half frame respectively includes: A main rod, with the bottom end fixed on the bottom plate and the top end extending upward; An inclined rod, with one end fixed to the top end of the main rod and the other end extending upward and obliquely outward; Among them, the inclined rods on the two half frames form the oblique frame, the rotating shaft of the roller is arranged between the two inclined rods, and one end of the rotating shaft is rotatably penetrated through one of the inclined rods, and the other end is coaxially connected to the output shaft of the first motor.

4. The cryopreservation device for a semen cryopreservation tube according to claim 3, characterized in that, The half frame further includes: An inclined strut, with one end fixedly connected to the main rod and the other end extending obliquely toward the bottom plate and fixed on the bottom plate; A fixing rod, fixed on the bottom plate, and one end of the fixing rod is connected to the inclined strut, and the other end is fixed to the main rod; Among them, the inclined strut, the fixing rod and the main rod form a triangle.

5. The cryopreservation device for a semen cryopreservation tube according to claim 3, wherein, The main rod of at least one side of the bracket is gradually inclined outward from top to bottom.

6. The cryopreservation device for a semen cryopreservation tube according to claim 1, characterized in that, At least two rollers are arranged on each of the oblique frames from top to bottom, and the rollers on the two oblique frames are arranged in one-to-one correspondence left and right; At least one of the brackets can move left and right in the horizontal direction to approach or move away from the other bracket, so as to guide the cryopreservation tubes to move downward synchronously when rotating between the two oblique frames.

7. The cryopreservation device for a semen cryopreservation tube according to claim 6, characterized in that, The bracket is connected to a second motor through a traction rope. When the second motor rotates, the traction rope is wound around the output shaft of the second motor, thereby driving the bracket to move.

8. The cryopreservation device for a semen cryopreservation tube according to claim 7, characterized in that, The second motor is connected to a controller, and the controller controls the moving distance of the bracket by controlling the second motor.

9. The cryopreservation device for a semen cryopreservation tube according to claim 1, characterized in that, The rotating surface of the roller is provided with an anti-slip structure; The anti-slip structure is an anti-slip strip arranged on the rotating surface, and the anti-slip strip extends along the axial direction of the rotating surface and is arranged at intervals along the circumferential direction of the rotating surface in multiple numbers.

10. The cryopreservation device for a semen cryopreservation tube according to claim 1, characterized in that, A liquid nitrogen level mark is arranged on the shown bracket, and the liquid nitrogen level mark is used to indicate the liquid level of liquid nitrogen.

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