Heat-preservation and light-shielding flow type incubation box
By designing a flow incubation box containing the inner box body, the outer box body and the lid, the problem of troublesome and waste of resources in the laboratory of flow cytometry in the light and constant temperature incubation is solved, and efficient light and constant temperature incubation is achieved, and experimental efficiency is improved.
Patent Information
- Application Number
- CN202421528993.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-01
- Publication Date
- 2025-04-25
- Estimated Expiration
- 2034-07-01
AI Technical Summary
The light-proof and constant temperature incubation methods of flow cytometry in the laboratory are troublesome and waste resources. The existing methods require frequent use of aluminum foil and back-to-back search of constant temperature equipment, which reduces the experimental efficiency.
A flow incubation box that is insulated and light-proof is designed, including the inner box body, the outer box body and the box cover. A flow tube frame is installed in the inner box body, which is fixed in the outer box body through a snap connection mechanism, and a thermal insulation material placement area is set in the outer box body to achieve a constant temperature effect.
Effective light-proof and constant temperature incubation of flow tubes is achieved, which improves experimental efficiency, reduces the consumption of aluminum foil, and simplifies experimental operations.
Smart Images

Figure CN222800449U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to a biological cell culture device, in particular to a heat-insulating and light-shielding flow incubation box. Background Art
[0002] Flow cytometry is a commonly used experimental method in molecular biology laboratories and is widely used in immunology and oncology. In the early preparation of flow cytometry, cells generally need to be placed in flow tubes, and go through centrifugation, rinsing, staining, fixation and other steps. In this series of processes, the flow tubes are usually placed on flow tube racks and incubated in the dark for a period of time at a specific temperature (mostly 0-4°C or 37°C) according to experimental requirements. At present, the common method in laboratories is to wrap the flow tube rack loaded with flow tubes with aluminum foil to achieve the purpose of light protection, and then place it in a refrigerator or incubator.
[0003] The current problem is:
[0004] Question 1: The commonly used method of light-shielding in laboratories is to wrap the flow tube rack containing the flow tubes with aluminum foil, which is very troublesome and the aluminum foil is rarely recycled, which is very wasteful.
[0005] Question 2: The commonly used flow cytometry constant temperature incubation method in the laboratory is to place the flow tube rack loaded with flow tubes in a refrigerator or incubator. It is necessary to go back and forth between different laboratories many times to find relevant instruments, which is very troublesome and reduces the experimental efficiency. Summary of the invention
[0006] The utility model aims to provide a heat-insulating and light-shielding flow incubation box, which can achieve a light-shielding effect for the flow tubes therein and can conveniently perform ice bath or constant temperature incubation, thereby improving the experimental efficiency.
[0007] In order to achieve the above technical purpose, the utility model adopts the following technical solutions:
[0008] A heat-insulating and light-shielding flow incubation box, comprising an inner box body, an outer box body and a box cover; a flow tube rack is arranged in the inner box body, and the inner box body is placed in the outer box body; a snap connection mechanism is arranged between the inner box body and the outer box body, and the inner box body is assembled inside the outer box body through the snap connection mechanism; and the box cover is closed on the outer box body.
[0009] Furthermore, a heat-insulating material placement area is provided between the inner box body and the outer box body, and heat-insulating material is placed in the heat-insulating material placement area.
[0010] Furthermore, the thermal insulation material is crushed ice or warm water.
[0011] Furthermore, the inner box body is placed in the outer box body, and its specific structural form is: a seating edge frame is provided on the inner side wall of the outer box body, and the inner box body is placed into the outer box body, and the bottom of the inner box body is seated on the seating edge frame in the outer box body.
[0012] Furthermore, the snap connection mechanism is specifically implemented in the form of an embedding strip on the inner side wall of the outer box body, and an embedding groove on the outer side wall of the inner box body, and the embedding strip of the outer box body can be embedded in the embedding groove of the inner box body, thereby forming the snap connection mechanism.
[0013] Furthermore, the outer box body and the box cover are both sandwich structures, and heat-insulating materials are arranged in the sandwich.
[0014] Furthermore, the thermal insulation material is a rock wool thermal insulation board or foam glass.
[0015] Compared with the prior art, the flow incubation box of the utility model has the following beneficial effects:
[0016] 1) In the flow incubation box of the present invention, the outer box body and the box cover are both made of opaque materials, so that the light shielding effect can be achieved for the flow tube, thereby improving the experimental efficiency and reducing the consumption of aluminum foil;
[0017] 2) In the flow incubation box of the present invention, placing the insulation material in the insulation material placement area can achieve the insulation effect, and ice bath or constant temperature incubation can be more conveniently performed during the flow incubation process, thereby improving the experimental efficiency. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 It is a schematic structural diagram of the heat-insulating and light-shielding flow incubation box of the utility model. DETAILED DESCRIPTION
[0019] The utility model is further described below with specific embodiments:
[0020] This embodiment provides a heat-insulating and light-shielding flow incubation box, which is used to place a flow tube 1 to simplify the flow cytometry process.
[0021] See also Figure 1 The flow incubation box of this embodiment includes an inner box body 2, an outer box body 3 and a box cover 4.
[0022] The inner box body 2 is provided with a flow tube rack 21, and the inner box body 2 is used to place the flow tube 1. It should be noted that the structure of the flow tube rack 21 provided in the inner box body 2 is basically the same as that of a conventional flow tube rack, so the specific structure of the flow tube rack is not described in detail herein.
[0023] The structural dimensions of the inner box body 2 match those of the outer box body 3 , and the inner box body 2 is placed inside the outer box body 3 .
[0024] Specifically, a seating edge frame 33 is provided on the inner side wall of the outer box body 3, and the inner box body 2 is placed into the outer box body 3, and the bottom of the inner box body 2 is seated on the seating edge frame 33 inside the outer box body 3, thereby realizing "the inner box body 2 is placed inside the outer box body 3".
[0025] The seating edge frame 33 is at a certain distance from the inner bottom surface of the outer box body 3, which is about 10 cm. The purpose of setting this distance is to free up the space at the bottom of the outer box body 3 as a heat preservation material placement area.
[0026] It should be noted that the seating edge frame 33 is actually a boss protruding outward from the inner side wall of the outer box body 3 .
[0027] In addition, a snap-fit connection mechanism is provided between the inner box body 2 and the outer box body 3 , through which the inner box body 2 can be assembled inside the outer box body 3 .
[0028] Specifically, an embedding strip 31 is provided on the inner side wall of the outer box body 3, and the embedding strip 31 is located above the seating edge frame 33 and adjacent to the seating edge frame 33; an embedding groove is provided on the outer side wall of the inner box body 2, and the embedding groove is adjacent to the bottom of the inner box body 2, and the embedding groove corresponds to the embedding strip 31 provided on the inner side wall of the outer box body 3, and the configurations of the two match each other; the inner box body 2 is placed in the outer box body 3, and the bottom of the inner box body 2 is seated on the seating edge frame 33 in the outer box body 3, and at the same time, the "embedding strip 31 on the inner side wall of the outer box body 3" is embedded in the "embedding groove on the outer side wall of the inner box body 2", thereby forming a snap-on connection effect, that is, realizing the function of the snap-on connection mechanism.
[0029] It should be noted that the embedding strip 31 is actually a convex strip protruding outward from the inner side wall of the outer box body 3.
[0030] It should be noted that, since the seating edge frame 33 is at a certain distance from the inner bottom surface of the outer box body 3, when the inner box body 2 is placed in the outer box body 3, there is a space between the bottom surface of the inner box body 2 and the bottom surface of the outer box body 3, and the space is used to place thermal insulation material, so the space is called "thermal insulation material placement area 32".
[0031] The box cover 4 is used to cover the outer box body 3, specifically, to cover the upper opening of the outer box body 3, so as to form a closed flow tube storage container;
[0032] The specific method of using the flow incubation box of this embodiment is as follows:
[0033] Open the box cover 4, take the inner box body 2 out of the outer box body 3, put the pre-prepared flow tube 1 into the flow tube rack 21 in the inner box body 2, add insulation material, such as crushed ice or warm water, into the outer box body 3, put the inner box body 2 back into the outer box body 3, and snap-connect the inner box body 2 and the outer box body 3 together through a snap-connecting mechanism; at this time, the previously added insulation material is in the insulation material placement area 32 between the inner box body 2 and the outer box body 3, so as to control the ambient temperature of the flow tube 1, so that the specific requirements of some flow cytometry for ambient temperature can be met; finally, close the box cover 4 on the upper opening of the outer box body 3, so as to encapsulate the flow tube 1 in a closed environment.
[0034] Since the flow tube 1 is in a closed environment composed of the outer box body 3 and the box cover 4, it is convenient for the flow tube 1 to be placed and transported in a light-proof and constant temperature environment.
[0035] It should be noted that the inner box body 2, the outer box body 3 and the box cover 4 are all made of opaque materials, so that the light-proof requirements of certain flow cytometry techniques can be met.
[0036] It should be noted that the outer box body 3 and the box cover 4 are both sandwich structures, and thermal insulation materials such as rock wool insulation board, foam glass, etc. are arranged in the sandwich, which is conducive to maintaining a constant temperature of the environment where the flow tube 1 is located.
[0037] It should be noted that the dimensions and configurations of the inner box body 2, the outer box body 3, and the box cover 4 match those of a "standard 96-well plate flow tube rack", and the long diameter and wide diameter of the outer box body 3 are 122 mm and 85 mm, respectively, so as to facilitate fixing on a shaker for oscillation.
[0038] The flow incubation box of this embodiment has the following advantages:
[0039] 1) In the flow incubation box of the present embodiment, an outer box body 3 and a box cover 4 are provided on the basis of the original flow tube rack, and both the outer box body 3 and the box cover 4 are made of opaque materials. In this way, light protection is more convenient during the flow incubation process, the experimental efficiency is improved, and the consumption of aluminum foil is reduced, thereby solving the problem 1 mentioned in the background technology that "the commonly used flow light protection method in the laboratory is to wrap the flow tube rack loaded with flow tubes with aluminum foil, which is very troublesome, and the aluminum foil is generally rarely recycled, which is very wasteful";
[0040] 2) In the flow incubation box of the present embodiment, an insulation material placement area 32 is provided on the basis of the original flow tube rack. Placing insulation material in the insulation material placement area 32 can achieve insulation effect. In this way, ice bath or constant temperature incubation can be more convenient during the flow incubation process, which improves the experimental efficiency and solves the problem 2 mentioned in the background technology: "The commonly used flow constant temperature incubation method in the laboratory is to place the flow tube rack loaded with flow tubes in a refrigerator or incubator. It is necessary to go back and forth many times in different laboratories to find related instruments, which is very troublesome and reduces the experimental efficiency."
[0041] The above are only preferred embodiments of the present invention and are not intended to limit the scope of protection of the present invention. Therefore, any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.
Claims
1. A heat-insulating and light-shielding flow incubation box, characterized in that: The flow incubation box comprises an inner box body (2), an outer box body (3) and a box cover (4); A flow tube rack (21) is arranged in the inner box body (2), and the inner box body (2) is placed in the outer box body (3); A snap-fit connection mechanism is provided between the inner box body (2) and the outer box body (3), and the inner box body (2) is assembled inside the outer box body (3) through the snap-fit connection mechanism; The box cover (4) covers the outer box body (3).
2. The heat-insulating and light-shielding flow incubation box according to claim 1, characterized in that: A heat-insulating material placement area (32) is provided between the inner box body (2) and the outer box body (3), and heat-insulating material is placed in the heat-insulating material placement area (32).
3. The heat-insulating and light-shielding flow incubation box according to claim 2, characterized in that: The heat-insulating material is crushed ice or warm water.
4. The heat-insulating and light-shielding flow incubation box according to claim 1, characterized in that: The inner box body (2) is placed inside the outer box body (3), and its specific structural form is: a seating edge frame (33) is provided on the inner side wall of the outer box body (3), and the inner box body (2) is placed inside the outer box body (3), and the bottom of the inner box body (2) is seated on the seating edge frame (33) inside the outer box body (3).
5. The heat-insulating and light-shielding flow incubation box according to claim 1, characterized in that: The buckle connection mechanism is specifically implemented in the following structural form: an embedding strip (31) is provided on the inner side wall of the outer box body (3), and an embedding groove is provided on the outer side wall of the inner box body (2); the embedding strip (31) of the outer box body (3) can be embedded in the embedding groove of the inner box body (2), thereby forming the buckle connection mechanism.
6. The heat-insulating and light-shielding flow incubation box according to claim 1, characterized in that: The outer box body (3) and the box cover (4) are both sandwich structures, and heat-insulating materials are arranged in the sandwich.
7. The heat-insulating and light-shielding flow incubation box according to claim 6, characterized in that: The thermal insulation material is rock wool thermal insulation board or foam glass.