Fixing device for bacterial endotoxin gel assay

Abstract

The utility model discloses a fixing device used in a bacterial endotoxin gel determination method, which comprises a test tube rack, the test tube rack comprises an upper plate and a lower plate, the outer surface of the upper plate is provided with placing holes, the outer surface of the lower plate is provided with flow guide holes, the upper surface of the upper plate is provided with a multifunctional test tube clamp, and the upper plate is provided with a clamping groove. The multifunctional test tube clamp comprises a test tube clamp body and an upper cover, the test tube clamp body comprises a frame body and a clamping mechanism, a rectangular groove is formed in the frame body, the clamping mechanism is located in the rectangular groove, the clamping mechanism comprises a connecting plate and a clamping plate, the side inner wall of the rectangular groove is fixedly connected with the connecting plate, and the clamping plate is located in the rectangular groove. The side surface of the connecting plate is fixedly connected with the clamping plates, the two clamping plates are symmetrically distributed, the outer side of a test tube can be rapidly clamped through the clamping mechanism, and the situation that a sample shakes to interfere with an experiment result in the process that the sample tube enters the constant-temperature water bath box is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of toxin detection, in particular to a fixing device used in a bacterial endotoxin gel determination method. Background Art

[0002] Bacterial endotoxin inspection is an important quality indicator to ensure the safety of injections. The bacterial endotoxin gel method test in a constant temperature water bath environment is a commonly used detection method. The traditional bacterial endotoxin gel method test is to place the sample tube in an environment of 37±0.2° (constant temperature water bath) and perform the test based on the principle of agglutination reaction between horseshoe crab reagent and endotoxin. The endotoxin content of the sample is determined by observing the gel state of the sample tube taken out of the thermostat and slowly rotated 180°.

[0003] The disadvantages of existing detection methods are:

[0004] 1. Using an ordinary test tube rack to place the sample tube into the constant temperature water bath will cause the sample to shake and interfere with the experimental results.

[0005] 2. During the experiment, the opening of the sample tube will cause impurities to enter and affect the experimental results.

[0006] 3. To check the experimental results, the experimenter needs to flip the sample tube by hand. The experimenter can observe up to 4 sample tubes at the same time, and the experimental results cannot be compared intuitively. Utility Model Content

[0007] The utility model aims to solve at least one of the technical problems existing in the prior art, and provides a fixing device used in a bacterial endotoxin gel determination method. By arranging eight clamping mechanisms inside each frame, eight sample tubes can be used to view the experimental results at the same time, and the comparison results are more intuitive. The outer side of the test tube can be quickly clamped by the clamping mechanism. When the lower end of the test tube passes through the clamping mechanism, the bottom end of the test tube will be squeezed with the clamping plate, and then the clamping plate drives the connecting plate to produce elastic deformation outward. When the lower end of the test tube passes through the placement hole and enters the test tube rack, the height of the protruding part of the test tube on the upper plate is clamped and fixed by the clamping plate, so that the sample shaking during the sample tube entering the constant temperature water bath will not interfere with the experimental results. In addition, a guide hole is arranged on the lower plate of the test tube rack, so that the test tube rack can be placed in water smoothly when it is placed in water, reducing water flow fluctuations. The upper cover and the cylinder body can prevent impurities from entering the sample tube and affecting the experimental results, and the upper cover can be installed and disassembled in conjunction with the clamping mechanism, and the operation is simple and convenient.

[0008] The utility model also provides a fixing device used for the above-mentioned bacterial endotoxin gel determination method, including: a test tube rack, the test tube rack including an upper plate and a lower plate, the outer surface of the upper plate is provided with a placement hole, the outer surface of the lower plate is provided with a guide hole, the upper surface of the upper plate is provided with a multifunctional test tube clamp, the multifunctional test tube clamp includes a test tube clamp and an upper cover, the test tube clamp includes a frame body and a clamping mechanism, the interior of the frame body is provided with a rectangular groove, the clamping mechanism is located inside the rectangular groove, the clamping mechanism includes a connecting plate and a clamping plate, the side inner wall of the rectangular groove is fixedly connected to the connecting plate, the side surface of the connecting plate is fixedly connected to the clamping plate, the number of the clamping plates is two and they are symmetrically distributed, and the lower surface of the upper cover is provided with a snap-on mechanism.

[0009] According to the fixing device used in the bacterial endotoxin gel assay, the lower surface of the upper plate is fixedly connected to the side plate, and the upper surface of the lower plate is fixedly connected to the side plate.

[0010] According to the fixing device used in the bacterial endotoxin gel assay, the number of the placement holes and the flow guide holes are both eighty and in a rectangular array, and the placement holes are located directly below the clamping plate.

[0011] According to the fixing device used in the bacterial endotoxin gel assay, the number of the frames is ten, each of the frames comprises eight rectangular grooves, and a handle is fixedly connected to the side surface of the frame.

[0012] According to the fixing device used in the bacterial endotoxin gel assay, the upper surface of the upper cover is provided with a cylinder, and the lower surface of the upper cover is in contact with the upper surface of the frame.

[0013] According to the fixing device used in the bacterial endotoxin gel assay method, the clamping mechanism includes a mounting plate, a clamping block and a limiting column, the upper surface of the upper cover is fixedly connected to the mounting plate, the side surface of the mounting plate is fixedly connected to the clamping block, the side surface of the mounting plate is fixedly connected to the limiting column, the side inner wall of the frame is provided with a clamping groove, and the clamping block and the clamping groove are adapted to each other.

[0014] According to the fixing device used in the bacterial endotoxin gel assay, the side surface of the frame is provided with a limiting groove, and the limiting column and the limiting groove are adapted to each other.

[0015] According to the fixing device used in the bacterial endotoxin gel assay, an insert block is fixedly connected to the lower surface of the upper cover, and an elongated hole is provided on the upper surface of the frame, and the insert block and the elongated hole are adapted to each other.

[0016] Additional aspects and advantages of the present invention will be given in part in the following description, and in part will become apparent from the following description, or will be learned through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The utility model is further described below in conjunction with the accompanying drawings and embodiments;

[0018] Figure 1 This is a schematic diagram of the overall structure of a fixing device used in a bacterial endotoxin gel assay method of the utility model;

[0019] Figure 2 This is a schematic diagram of the test tube rack structure of a fixing device used in a bacterial endotoxin gel determination method of the utility model;

[0020] Figure 3 This is a schematic diagram of the frame structure of a fixing device used in a bacterial endotoxin gel determination method of the utility model;

[0021] Figure 4 This is a schematic diagram of the upper cover structure of a fixing device used in a bacterial endotoxin gel assay of the utility model;

[0022] Figure 5 The utility model is a schematic cross-sectional view of a frame of a fixing device used in a bacterial endotoxin gel determination method.

[0023] Legend:

[0024] 1. Test tube rack; 2. Upper plate; 3. Lower plate; 4. Placement hole; 5. Guide hole; 6. Side plate; 7. Frame; 8. Connecting plate; 9. Clamping plate; 10. Handle; 11. Upper cover; 12. Cylinder; 13. Mounting plate; 14. Block; 15. Limiting column; 16. Slot; 17. Limiting slot; 18. Insert block; 19. Long hole. DETAILED DESCRIPTION

[0025] This section will describe in detail the specific embodiments of the utility model. The preferred embodiments of the utility model are shown in the accompanying drawings. The purpose of the accompanying drawings is to supplement the description of the text part of the specification with graphics, so that people can intuitively and vividly understand each technical feature and the overall technical solution of the utility model, but it cannot be understood as a limitation on the protection scope of the utility model.

[0026] Reference Figure 1-5, the utility model embodiment of a fixing device used in a bacterial endotoxin gel assay method, comprising: a test tube rack 1, the test tube rack 1 comprising an upper plate 2 and a lower plate 3, the lower surface of the upper plate 2 is fixedly connected to a side plate 6, the upper surface of the lower plate 3 is fixedly connected to the side plate 6, the outer surface of the upper plate 2 is provided with a placement hole 4, the outer surface of the lower plate 3 is provided with a guide hole 5, the placement holes 4 distributed on the upper and lower sides coincide with the axes of the guide holes 5, and the number of the placement holes 4 and the guide holes 5 are both eighty and in a rectangular array, the lateral distance between the holes is 20 mm, the longitudinal distance is 27 mm, the minimum distance between the upper plate 2 and the lower plate 3 is 16.5 mm, the placement hole 4 is located directly below the clamping plate 9, the test tube can pass through the placement hole 4 into between the upper plate 2 and the lower plate 3, for supporting the test tube, the guide hole 5 is used to allow the test tube rack 1 to be placed smoothly in water, reduce water flow fluctuations, and the eighty placement holes 4 can simultaneously place eighty test tube samples, thereby increasing the test tube capacity of the device.

[0027] A multifunctional test tube clamp is arranged on the upper surface of the upper plate 2, and the multifunctional test tube clamp includes a test tube clamp and an upper cover 11. The test tube clamp includes a frame 7 and a clamping mechanism. A rectangular groove is arranged inside the frame 7, and the clamping mechanism is located inside the rectangular groove. The clamping mechanism includes a connecting plate 8 and a clamping plate 9. The side inner wall of the rectangular groove is fixedly connected to the connecting plate 8, and the side surface of the connecting plate 8 is fixedly connected to the clamping plate 9. The number of clamping plates 9 is two and they are symmetrically distributed. The number of frames 7 is ten, and the number of rectangular grooves included in each frame 7 is eight. The side surface of the frame 7 is fixed. A handle 10 is connected. Before the test tube passes through the placement hole 4, the bottom end of the test tube will be squeezed with the clamping plate 9, and then the clamping plate 9 drives the connecting plate 8 to produce elastic deformation outward. When the lower end of the test tube is placed in the test tube rack 1, the height of the protruding part of the test tube on the upper plate 2 is clamped and fixed by the clamping plate 9, so as to avoid the sample shaking and interfering with the experimental results when the sample tube enters the constant temperature water bath, and the operation is simple and convenient; and when it is necessary to view the experimental results, the frame 7 can be turned over and eight sample tubes can be observed at the same time. The comparison results are more intuitive and have good practicality.

[0028] The upper surface of the upper cover 11 is provided with a cylinder 12, and the lower surface of the upper cover 11 is in contact with the upper surface of the frame 7. The upper cover 11 and the cylinder 12 are used together to prevent impurities from entering the opening of the test tube, thereby affecting the experimental results.

[0029] The lower surface of the upper cover 11 is provided with a clamping mechanism, which includes a mounting plate 13, a clamping block 14 and a limiting column 15. The upper surface of the upper cover 11 is fixedly connected to the mounting plate 13, the side surface of the mounting plate 13 is fixedly connected to the clamping block 14, the side surface of the mounting plate 13 is fixedly connected to the limiting column 15, the side inner wall of the frame 7 is provided with a clamping groove 16, the clamping block 14 and the clamping groove 16 are adapted to each other, the side surface of the frame 7 is provided with a limiting groove 17, the limiting column 15 and the limiting groove 17 are adapted to each other, the lower surface of the upper cover 11 is fixedly connected with an insert block 18, and the upper surface of the frame 7 is provided with The long hole 19 and the insert block 18 are adapted to each other. When the upper cover 11 needs to be installed, the insert block 18 is aligned with the long hole 19 and pressed downward. The inclined surface of the block 14 is first squeezed with the side wall of the frame 7 and deformed toward the inner side of the upper cover 11. When the insert block 18 is completely embedded in the long hole 19, the block 14 can just restore the deformation and engage with the slot 16. At this time, the limit column 15 is located in the limit groove 17, and the fixation of the upper cover 11 and the frame 7 is completed; when the upper cover 11 needs to be removed, press the limit column 15, and then pull the upper cover 11 out of the frame 7. The operation is simple and convenient.

[0030] Working principle: when the device needs to be used, first pass the lower end of the test tube through the clamping mechanism, the bottom end of the test tube will be squeezed with the clamping plate 9, and then the clamping plate 9 drives the connecting plate 8 to produce elastic deformation outward. When the lower end of the test tube passes through the placement hole 4 and enters the test tube rack 1, the height of the protruding part of the upper plate 2 of the test tube is clamped and fixed by the clamping plate 9, and then the upper cover 11 is installed, and the plug block 18 is aligned with the long hole 19 and pressed downward. The inclined surface of the clamping block 14 is first squeezed with the side wall of the frame 7 and deformed toward the inner side of the upper cover 11. When the plug block 18 is completely embedded in the long hole 19, the clamping block 14 can just restore the deformation and engage with the slot 16. At this time, the limiting column 15 is located in the limiting slot 17, that is, the fixation of the upper cover 11 and the frame 7 is completed, and when it is necessary to view the experimental results, the frame 7 can be turned over and eight sample tubes can be observed at the same time. The comparison results are more intuitive and have good practicality.

[0031] The embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments. Various changes can be made within the knowledge scope of ordinary technicians in the technical field without departing from the purpose of the present invention.

Claims

1. A fixing device used in a bacterial endotoxin gel assay, characterized in that: include: A test tube rack (1), the test tube rack (1) comprising an upper plate (2) and a lower plate (3), the outer surface of the upper plate (2) being provided with a placement hole (4), the outer surface of the lower plate (3) being provided with a guide hole (5), the upper surface of the upper plate (2) being provided with a multifunctional test tube clamp, the multifunctional test tube clamp comprising a test tube clamp and an upper cover (11), the test tube clamp comprising a frame (7) and a clamping mechanism, the interior of the frame (7) being provided with a rectangular groove, the clamping mechanism being located inside the rectangular groove, the clamping mechanism comprising a connecting plate (8) and a clamping plate (9), the side inner wall of the rectangular groove being fixedly connected to the connecting plate (8), the side surface of the connecting plate (8) being fixedly connected to the clamping plate (9), the number of the clamping plates (9) being two and being symmetrically distributed, and the lower surface of the upper cover (11) being provided with a clamping mechanism.

2. The fixing device used in the bacterial endotoxin gel assay according to claim 1, characterized in that: The lower surface of the upper plate (2) is fixedly connected to the side plate (6), and the upper surface of the lower plate (3) is fixedly connected to the side plate (6).

3. The fixing device used in the bacterial endotoxin gel assay according to claim 1, characterized in that: The number of the placement holes (4) and the guide holes (5) are both eighty and arranged in a rectangular array. The placement holes (4) are located directly below the clamping plate (9).

4. The fixing device used in the bacterial endotoxin gel assay according to claim 1, characterized in that: The number of the frames (7) is ten, and each of the frames (7) comprises eight rectangular grooves. A handle (10) is fixedly connected to the side surface of the frame (7).

5. The fixing device used in the bacterial endotoxin gel assay according to claim 1, characterized in that: The upper surface of the upper cover (11) is provided with a cylinder (12), and the lower surface of the upper cover (11) and the upper surface of the frame (7) are in contact with each other.

6. The fixing device used in the bacterial endotoxin gel assay according to claim 1, characterized in that: The clamping mechanism comprises a mounting plate (13), a clamping block (14) and a limiting column (15); the upper surface of the upper cover (11) is fixedly connected to the mounting plate (13); the side surface of the mounting plate (13) is fixedly connected to the clamping block (14); the side surface of the mounting plate (13) is fixedly connected to the limiting column (15); a clamping groove (16) is provided on the side inner wall of the frame (7); the clamping block (14) and the clamping groove (16) are mutually adapted.

7. The fixing device used in the bacterial endotoxin gel assay according to claim 6, characterized in that: A limiting groove (17) is provided on the side surface of the frame (7), and the limiting column (15) and the limiting groove (17) are adapted to each other.

8. The fixing device used in the bacterial endotoxin gel assay according to claim 6, characterized in that: An insert block (18) is fixedly connected to the lower surface of the upper cover (11), and an elongated hole (19) is provided on the upper surface of the frame body (7), wherein the insert block (18) and the elongated hole (19) are adapted to each other.

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