Bifunctional electrochemical test strip

Abstract

The utility model provides a bifunctional electrochemical test strip which comprises a sample chamber and a plurality of electrodes, at least one part of each of the plurality of electrodes is positioned in the sample chamber, and the plurality of electrodes comprise a first auxiliary electrode, a second auxiliary electrode, a first working electrode, a second working electrode and a third auxiliary electrode, the first auxiliary electrode and the second auxiliary electrode form a current loop, and the third auxiliary electrode forms a current loop with the first auxiliary electrode, the first working electrode and the second working electrode respectively. Two indexes can be detected at the same time by adding a blood sample into the sample chamber, so that the detection efficiency is improved; by detecting the hematocrit and the background signal and correcting the detection of the first index and the second index according to the detection result, the detection accuracy is improved; and the detection of the two indexes adopts the same third auxiliary electrode, so that the volume of a sample chamber is reduced, and the blood consumption for detection is reduced.

Description

Technical Field

[0001] The utility model relates to the field of electrochemical detection, and more specifically to a dual-function electrochemical test strip. Background Art

[0002] In order to quickly detect indicators such as blood sugar, uric acid, cholesterol, etc. in the prior art, electrochemical test strips with corresponding functions have been launched on the market; however, the electrochemical test strips on the market are generally single-function and can only detect a single indicator; if multiple indicator detection is required, it is necessary to purchase electrochemical test strips with different functions, which brings a lot of inconvenience to users.

[0003] In order to solve this technical problem, the publication number CN216433974U provides a β-hydroxybutyrate and acetoacetic acid dual-function electrochemical detection test paper, including a substrate, and a first conductive layer for β-hydroxybutyrate detection and a second conductive layer for acetoacetic acid detection respectively arranged on the front and back of the substrate, the first conductive layer and the second conductive layer are respectively provided with double-sided adhesive layers, and each double-sided adhesive layer is provided with a groove at one end, wherein a β-hydroxybutyrate detection enzyme layer is provided in one groove, and an acetoacetic acid detection enzyme layer is provided in the other groove; a hydrophilic film is provided on each double-sided adhesive layer, and each hydrophilic film and the corresponding groove form a siphon channel located on the substrate; a spacer is provided on each double-sided adhesive layer and is in the same plane as the hydrophilic film; the connection electrode in the first conductive layer is connected to the working electrode; the connection electrode in the second conductive layer is connected to the working electrode and the counter electrode respectively. Although the above electrochemical detection test paper can detect two indicators, it requires two injections and has a low detection efficiency.

[0004] Therefore, the prior art has a large room for improvement. Utility Model Content

[0005] The purpose of the utility model is to make up for the deficiencies of the prior art and to provide a dual-function electrochemical test strip.

[0006] In order to achieve the above object, the utility model is implemented through the following technical solutions:

[0007] The utility model provides a dual-function electrochemical test strip, comprising a sample chamber and a plurality of electrodes, wherein at least a portion of the plurality of electrodes are located in the sample chamber, the plurality of electrodes comprise a first auxiliary electrode, a second auxiliary electrode, a first working electrode, a second working electrode and a third auxiliary electrode, the first auxiliary electrode and the second auxiliary electrode form a current loop, and the third auxiliary electrode respectively forms a current loop with the first auxiliary electrode, the first working electrode and the second working electrode.

[0008] The first auxiliary electrode and the second auxiliary electrode form a current loop for detecting the hematocrit (HCT); the third auxiliary electrode and the first working electrode form a current loop for detecting the first indicator; the third auxiliary electrode and the second working electrode form a current loop for detecting the second indicator; the third auxiliary electrode and the first auxiliary electrode form a current loop for detecting the background signal. Through the above-mentioned structural setting, two indicators can be detected at the same time by adding a blood sample to the sample chamber, thereby improving the detection efficiency; by detecting the hematocrit and the background signal, and correcting the detection of the first indicator and the second indicator according to the detection results, it is beneficial to improve the detection accuracy; the detection of the two indicators uses the same third auxiliary electrode, which is beneficial to reducing the volume of the sample chamber, thereby reducing the amount of blood used for detection.

[0009] According to the above solution, the surface of the first working electrode is covered with a first enzyme layer, and the first enzyme layer is located in the sample chamber. The above structural setting is conducive to improving the detection speed and accuracy.

[0010] It is understandable that the type of enzyme in the first enzyme layer is selected according to the detection index. For example, when the first index is uric acid, the enzyme in the first enzyme layer is an enzyme that reacts with uric acid.

[0011] According to the above solution, the second auxiliary electrode and the third auxiliary electrode are respectively arranged on two branches of the same electrode. By arranging the second auxiliary electrode and the third auxiliary electrode to be respectively arranged on two branches of the same electrode, the second auxiliary electrode and the first auxiliary electrode form a current loop while the third auxiliary electrode and the first auxiliary electrode form a current loop, which is conducive to simplifying the structure.

[0012] Of course, those skilled in the art may also configure the second auxiliary electrode and the third auxiliary electrode to be separately configured electrodes according to actual conditions.

[0013] According to the above solution, the first auxiliary electrode, the second auxiliary electrode, the first working electrode, the second working electrode and the third auxiliary electrode located in the sample chamber are arranged in sequence from the outside to the inside along the sample chamber.

[0014] Through the above-mentioned structural setting, the third auxiliary electrode is located at the innermost position of the sample chamber, and a current loop is formed through the third auxiliary electrode and the first auxiliary electrode, which is also used for full suction judgment; when the blood sample basically covers the sample chamber, it can touch the third auxiliary electrode and trigger the instrument to start detection, which effectively avoids the influence of insufficient sample suction on the detection result, thereby helping to improve the detection accuracy.

[0015] According to the above solution, the surfaces of the second working electrode and the third auxiliary electrode are covered with a second enzyme layer.

[0016] It is understandable that the type of enzyme in the second enzyme layer is selected according to the detection index. For example, when the second index is blood sugar, the enzyme in the second enzyme layer is an enzyme that reacts with blood sugar.

[0017] According to the above solution, it also includes a substrate, on which a conductor layer and an electrode layer are printed in sequence, and the electrode layer includes the plurality of electrodes.

[0018] According to the above scheme, the electrode layer is covered with an insulating layer, and a notch is provided on the insulating layer; the insulating layer is covered with a double-sided adhesive layer, and an opening corresponding to the notch is provided on the double-sided adhesive layer; the sample chamber is formed between the double-sided adhesive layer and the substrate. The notch on the insulating layer corresponds to the opening on the double-sided adhesive layer, so that the double-sided adhesive layer is assembled on the insulating layer to form a sample chamber, that is, the sample chamber is actually surrounded by the substrate, the notch, and the opening.

[0019] According to the above scheme, the double-sided adhesive layer is covered with a hydrophilic film, and the hydrophilic film is located above the notch. The suction of the blood sample is achieved by utilizing the hydrophilicity of the hydrophilic film covering the double-sided adhesive layer and the siphon effect of the sample chamber. By providing the hydrophilic film, the diffusion speed of the blood sample in the sample chamber can be increased, thereby improving the detection efficiency.

[0020] According to the above solution, the double-sided adhesive layer is also covered with a cover film.

[0021] According to the above solution, the conductor layer is a conductive silver paste layer.

[0022] The beneficial effects of the utility model are:

[0023] The utility model sets a plurality of electrodes, at least a part of which is located in the sample chamber, wherein the plurality of electrodes include a second auxiliary electrode, a first auxiliary electrode, a first working electrode, a second working electrode and a third auxiliary electrode, wherein the second auxiliary electrode forms a current loop with the first auxiliary electrode, and the third auxiliary electrode forms a current loop with the first working electrode and the second working electrode respectively; by adding a blood sample into the sample chamber, two indicators can be detected at the same time, thereby improving the detection efficiency; by detecting the hematocrit through the second auxiliary electrode and the first auxiliary electrode and correcting the detection of the first indicator and the second indicator according to the detection result, it is beneficial to improve the detection accuracy; the detection of the two indicators uses the same third auxiliary electrode, which is beneficial to reducing the volume of the sample chamber, thereby helping to reduce the amount of blood used for the detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a structural schematic diagram of the utility model;

[0025] Figure 2 yes Figure 1 A magnified view of part A;

[0026] Figure 3 It is a schematic diagram of the structure of the electrode layer in the utility model; wherein the dotted frame is the position corresponding to the sample chamber.

[0027] In the figure: 1, substrate; 2, wire layer; 3, electrode layer; 31, first auxiliary electrode; 32, second auxiliary electrode; 33, first working electrode; 331, first enzyme layer; 34, second working electrode; 341, second enzyme layer; 35, third auxiliary electrode; 4, insulating layer; 41, notch; 5, double-sided adhesive layer; 51, opening; 6, hydrophilic film; 7, cover film. DETAILED DESCRIPTION

[0028] The embodiments of the present invention are described in detail below with reference to the accompanying drawings. However, the present invention can be implemented in many different ways as defined and covered by the claims.

[0029] In order to facilitate the understanding of the technical solution, in this embodiment, the first indicator is limited to uric acid and the second indicator is blood sugar. Of course, in other embodiments, those skilled in the art can make selections according to actual needs.

[0030] like Figure 1-3 As shown, a dual-function electrochemical test strip described in the utility model includes a sample chamber and a plurality of electrodes, at least a portion of the plurality of electrodes are located in the sample chamber, the plurality of electrodes include a first auxiliary electrode 31, a second auxiliary electrode 32, a first working electrode 33, a second working electrode 34 and a third auxiliary electrode 35, the first auxiliary electrode 31 and the second auxiliary electrode 32 form a current loop, and the third auxiliary electrode 35 forms a current loop with the first auxiliary electrode 31, the first working electrode 33, and the second working electrode 34 respectively.

[0031] The first auxiliary electrode 31 and the second auxiliary electrode 32 form a current loop for detecting the hematocrit; the third auxiliary electrode 35 and the first working electrode 33 form a current loop for detecting uric acid; the third auxiliary electrode 35 and the second working electrode 34 form a current loop for detecting blood sugar; the third auxiliary electrode 35 and the first auxiliary electrode 31 form a current loop for detecting background signals. Through the above-mentioned structural setting, two indicators can be detected at the same time by adding a blood sample to the sample chamber, thereby improving the efficiency of the detection; the hematocrit and the background signal are detected, and the detection of uric acid and blood sugar is corrected according to the detection results, which is conducive to improving the detection accuracy; the detection of two indicators uses the same third auxiliary electrode 35, which is conducive to reducing the volume of the sample chamber, thereby helping to reduce the amount of blood used for the detection.

[0032] Furthermore, the surface of the first working electrode 33 is covered with a first enzyme layer 331, and the first enzyme layer 331 is located in the sample chamber. The above structure is helpful to improve the detection speed and accuracy. The enzyme in the first enzyme layer 331 is an enzyme that reacts with uric acid.

[0033] Furthermore, the second auxiliary electrode 32 and the third auxiliary electrode 35 are respectively arranged on two branches of the same electrode.

[0034] Specifically, the first auxiliary electrode 31 , the second auxiliary electrode 32 , the first working electrode 33 , the second working electrode 34 and the third auxiliary electrode 35 located in the sample chamber are sequentially arranged from outside to inside along the sample chamber.

[0035] Through the above-mentioned structural setting, the third auxiliary electrode 35 is located at the innermost position of the sample chamber, and a current loop is formed through the third auxiliary electrode 35 and the first auxiliary electrode 31, which is also used for full suction judgment; when the blood sample basically covers the sample chamber, it can touch the third auxiliary electrode 35 and trigger the instrument to start detection, which effectively avoids the influence of insufficient sample suction on the detection result, thereby helping to improve the detection accuracy.

[0036] Furthermore, the surfaces of the second working electrode 34 and the third auxiliary electrode 35 are covered with a second enzyme layer 341. The enzyme in the second enzyme layer 341 is an enzyme that reacts with blood sugar.

[0037] Furthermore, it also includes a substrate 1, on which a wire layer 2 and an electrode layer 3 are printed in sequence, and the electrode layer 3 includes the plurality of electrodes.

[0038] Specifically, the electrode layer 3 is covered with an insulating layer 4, and a notch 41 is provided on the insulating layer 4; the insulating layer 4 is covered with a double-sided adhesive layer 5, and an opening 51 corresponding to the notch 41 is provided on the double-sided adhesive layer 5; the sample chamber is formed between the double-sided adhesive layer 5 and the substrate 1. The notch 41 on the insulating layer 4 corresponds to the opening 51 on the double-sided adhesive layer 5, so that the double-sided adhesive layer 5 is assembled on the insulating layer 4 to form a sample chamber, that is, the sample chamber is actually surrounded by the substrate 1, the notch 41, and the opening 51.

[0039] Furthermore, the double-sided adhesive layer 5 is covered with a hydrophilic film 6, and the hydrophilic film 6 is located above the notch 41. The suction of the blood sample is achieved by utilizing the hydrophilicity of the hydrophilic film 6 covering the double-sided adhesive layer 5 and the siphon effect of the sample chamber. By providing the hydrophilic film 6, the diffusion speed of the blood sample in the sample chamber can be increased, thereby improving the detection efficiency.

[0040] Furthermore, the double-sided adhesive layer 5 is also covered with a cover film 7 .

[0041] Furthermore, the conductor layer 2 is a conductive silver paste layer.

[0042] When the dual-function electrochemical test strip of the utility model is in use, the blood sample enters the sample chamber from the injection port of the sample chamber, and flows from the outside to the inside until it touches the third auxiliary electrode 35 located innermost in the sample chamber, and the third auxiliary electrode 35 forms a current loop with the first auxiliary electrode 31, thereby triggering the instrument to start detection; the third auxiliary electrode 35 forms a current loop with the first working electrode 33 for detecting uric acid; the third auxiliary electrode 35 forms a current loop with the second working electrode 34 for detecting blood sugar; the second auxiliary electrode 32 forms a current loop with the first auxiliary electrode 31 for detecting hematocrit; the third auxiliary electrode 35 forms a current loop with the first auxiliary electrode 31, and is also used to detect background signals; the detection of uric acid and blood sugar is corrected according to the detection results of hematocrit and background signals.

[0043] The above description is only a preferred embodiment of the present utility model, so all equivalent changes or modifications made according to the structure, features and principles described in the scope of the present utility model patent application are included in the scope of the present utility model patent application.

Claims

1. A dual-function electrochemical test strip, comprising a sample chamber and a plurality of electrodes, wherein at least a portion of each of the plurality of electrodes is located in the sample chamber, characterized in that: The plurality of electrodes include a first auxiliary electrode (31), a second auxiliary electrode (32), a first working electrode (33), a second working electrode (34) and a third auxiliary electrode (35); The second auxiliary electrode (32) and the first auxiliary electrode (31) form a current loop; The third auxiliary electrode (35) forms a current loop with the first auxiliary electrode (31), the first working electrode (33) and the second working electrode (34) respectively.

2. The dual-function electrochemical test strip according to claim 1, characterized in that: The surface of the first working electrode (33) is covered with a first enzyme layer (331), and the first enzyme layer (331) is located in the sample chamber.

3. The dual-function electrochemical test strip according to claim 1, characterized in that: The second auxiliary electrode (32) and the third auxiliary electrode (35) are respectively arranged on two branches of the same electrode; or the second auxiliary electrode (32) and the third auxiliary electrode (35) are each separately arranged electrodes.

4. The dual-function electrochemical test strip according to claim 1, characterized in that: The first auxiliary electrode (31), the second auxiliary electrode (32), the first working electrode (33), the second working electrode (34) and the third auxiliary electrode (35) located in the sample chamber are arranged in sequence from outside to inside along the sample chamber.

5. The dual-function electrochemical test strip according to claim 4, characterized in that: The surfaces of the second working electrode (34) and the third auxiliary electrode (35) are covered with a second enzyme layer (341).

6. The dual-function electrochemical test strip according to any one of claims 1 to 5, characterized in that: It also comprises a substrate (1), on which a conductor layer (2) and an electrode layer (3) are printed in sequence, and the electrode layer (3) comprises the plurality of electrodes.

7. The dual-function electrochemical test strip according to claim 6, characterized in that: The electrode layer (3) is covered with an insulating layer (4), and a notch (41) is provided on the insulating layer (4); the insulating layer (4) is covered with a double-sided adhesive layer (5), and an opening (51) corresponding to the notch (41) is provided on the double-sided adhesive layer (5); the sample chamber is formed between the double-sided adhesive layer (5) and the substrate (1).

8. The dual-function electrochemical test strip according to claim 7, characterized in that: The double-sided adhesive layer (5) is covered with a hydrophilic film (6), and the hydrophilic film (6) is located above the notch (41).

9. The dual-function electrochemical test strip according to claim 8, characterized in that: The double-sided adhesive layer (5) is also covered with a cover film (7).

10. The dual-function electrochemical test strip according to claim 6, characterized in that: The conductor layer (2) is a conductive silver paste layer.

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