Automatic culture equipment for NK (Natural Killer) cells
By designing the NK cell automated culture equipment, the structure of the multi-layer storage cavity and partition flow chamber, combined with the clamping setting of the wetting device and the placing disk, the problem of easy contamination during sampling in existing equipment is solved, and a more efficient and safe cell culture is achieved.
Patent Information
- Application Number
- CN202420845590.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-23
- Publication Date
- 2025-05-02
- Estimated Expiration
- 2034-04-23
AI Technical Summary
Existing cell culture equipment can easily cause other groups of cells to be exposed to air when removing a single group of cells, increasing the risk of contamination and cell death.
An automated NK cell culture device was designed, using a multi-layer storage chamber and a partition flow chamber structure, maintaining the same temperature and humidity through a wetting device, and achieving rapid and contaminated cell sampling through the clamping setting of the placing disk and cover plate.
It effectively avoids cell death caused by environmental differences, reduces the risk of cells being exposed to air, and improves the success rate of cell culture and the convenience of equipment operation.
Smart Images
Figure CN222821574U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell culture equipment, in particular to an automatic NK cell culture equipment. Background Art
[0002] NK cells, or Natural Killer cells, are an important type of cell in the human immune system. They are a type of lymphocytes and are mainly found in the blood, lymphatic tissues, and certain organs. The main function of NK cells is to recognize and kill virus-infected cells and certain cancer cells. Unlike T cells, which need to recognize specific antigens in advance, NK cells can directly recognize and attack abnormal cells, so they are called "natural" killers.
[0003] NK cell therapy, full name natural killer cell therapy, is an important category of tumor immunotherapy and a form of adoptive cell therapy. NK cell therapy uses mononuclear cells separated from the patient's own peripheral blood cells, amplified and activated in vitro, and then intravenously infused to kill tumor cells by activating the body's specific immune response.
[0004] However, existing culture equipment requires multiple groups of cells to be placed in the same spatial environment for culture at the same time. This requires opening the culture equipment when taking a single group of cells. This process can easily expose other groups of cells to the air, increasing the possibility of contamination and thus leading to the death of the cultured cells. Utility Model Content
[0005] In view of the technical problems in the above-mentioned prior art, the utility model provides an automated NK cell culture device, the purpose of which is to solve the above-mentioned problems.
[0006] In order to achieve the above purpose, the utility model provides the following technical solutions:
[0007] An automated NK cell culture device comprises a box body, wherein a multi-layer storage cavity is arranged in the box body, each layer of the storage cavity comprises four storage chambers, a partition is vertically arranged in the middle of the box body, a flow chamber is opened in the partition, and ventilation holes are respectively opened on the left and right side walls of the flow chamber corresponding to the storage chambers in each layer of the storage chamber, a humidifier is arranged in the flow chamber corresponding to the partition on the upper side of the box body, the humidifier is connected to the flow chamber, placement openings are respectively opened on the side walls of the box body corresponding to the multiple storage chambers, a cover plate is hinged on the upper side of the placement opening, and a torsion spring is arranged at the hinge, a placement plate is movably arranged on the bottom side of the storage chamber, and when the cover is closed, the outer side of the placement plate is clamped with the lower side of the cover plate.
[0008] Preferably, a tension spring is provided at one end of the placement plate facing the inner wall of the storage chamber, one end of the tension spring is fixedly connected to the inner wall of the storage chamber, and the other end is fixedly connected to the end of the placement plate facing the inner wall of the storage chamber, and the placement plate is slidably connected to the lower side wall of the storage chamber.
[0009] Preferably, the tension strength of the tension spring is greater than the torsion strength of the torsion spring.
[0010] Preferably, a card connection plate is provided on the side of the placement tray facing the outside of the storage chamber corresponding to the cover plate, a card slot is provided on the inner side of the upper side of the card connection plate, and a card block matching with the card slot is provided on the inner side of the lower end of the cover plate corresponding to the card slot.
[0011] Preferably, a thermometer and hygrometer is also provided on the top of the box body, and a temperature and humidity sensor is provided on the top of the storage room located at the top of the box body, and the temperature and humidity sensor is electrically connected to the thermometer and hygrometer.
[0012] Preferably, transparent observation windows are provided on the front and rear side walls of the box body corresponding to each storage chamber.
[0013] Compared with the prior art, the utility model has the following beneficial effects:
[0014] The utility model provides an automatic NK cell culture device, which delivers the same temperature and humidity to the culture area in each storage chamber through a humidifier and a flow cavity of a partition, thereby avoiding cell death due to environmental differences;
[0015] When it is necessary to take out the cells, it is only necessary to pull the placement tray of the corresponding storage chamber. At this time, the card block on the lower side of the cover loses the card connection of the card slot, and the placement port is opened under the action of the torsion spring. The cell sample can be taken out by continuing to pull the placement tray. The removal is convenient and fast, and at the same time, it is avoided that the hands go deep into the storage chamber, and the pollution of the culture environment is reduced as much as possible. When closing the cover, first close the cover, and then push the placement tray in, so that the card slot and the card block are engaged, and the placement tray is fixed by the tension of the tension spring, so that the cover is fixed and closed, ensuring that the culture environment is consistent. The entire opening and closing process is convenient and quick to operate. At the same time, this process will not let other cells contact the air, reducing the pollution of the culture environment by external air, thereby reducing the possibility of cell death due to pollution. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Figure 1 This is a front internal schematic diagram of an automated NK cell culture device according to the utility model;
[0017] Figure 2 This is a side internal schematic diagram of an automated NK cell culture device described in the present invention.
[0018] In the figure: 1. box body; 11. placement opening; 12. cover plate; 13. torsion spring; 14. clamping block; 15. transparent observation window; 2. storage cavity; 3. storage chamber; 31. placement plate; 32. tension spring; 33. clamping plate; 34. clamping slot; 4. partition; 41. flow cavity; 42. vent; 5. humidifier; 6. thermometer and hygrometer; 61. temperature and humidity sensor. DETAILED DESCRIPTION
[0019] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0020] See also Figure 1 and 2 In the embodiment proposed in the present application, there is: an automated NK cell culture device, comprising a box body 1, wherein a multi-layer storage cavity 2 is arranged in the box body 1, and each layer of the storage cavity 2 includes four storage chambers 3. Transparent observation windows 15 are arranged at the front and rear side walls of the box body 1 corresponding to each storage chamber 3, so as to facilitate real-time observation of the cell culture status. A partition 4 is arranged vertically in the middle of the box body 1, and a flow cavity 41 is arranged in the partition 4. Ventilation holes 42 are respectively arranged on the left and right side walls of the flow cavity 41 corresponding to the storage chamber 3 in each layer of the storage cavity 2. A humidifier 5 is arranged on the upper side of the box body 1 corresponding to the flow cavity 41 of the partition 4, and the humidifier 5 is connected to the flow cavity 41. A placement opening 11 is respectively arranged on the side wall of the box body 1 corresponding to the plurality of storage chambers 3. A cover plate 12 is hinged on the upper side of the placement opening 11, and a torsion spring 13 is arranged at the hinge. A placement tray 31 is movably arranged on the bottom side of the storage chamber 3. When the cover is closed, the outer side of the placement tray 31 is snap-fitted with the lower side of the cover plate 12;
[0021] The humidifier 5 cooperates with the flow cavity 41 of the partition 4 to deliver the same temperature and humidity to the culture area in each storage chamber 3, thereby avoiding cell death due to environmental differences. At the same time, the placement plate 31 and the cover plate 12 are arranged to be clamped together. When the cell sample is taken out, this process will not allow other cells to contact the air, thereby reducing the pollution of the culture environment by the external air, and the entire opening and closing process is convenient and quick to operate, thereby reducing the possibility of cell death due to pollution.
[0022] In another embodiment, see Figure 1 and 2The end of the placement plate 31 facing the inner wall of the storage chamber 3 is provided with a tension spring 32, one end of the tension spring 32 is fixedly connected to the inner wall of the storage chamber 3, and the other end is fixedly connected to the end of the placement plate 31 facing the inner wall of the storage chamber 3, and the placement plate 31 is slidably connected to the inner lower side wall of the storage chamber 3; a clamping plate 33 is provided on the side of the placement plate 31 facing the outside of the storage chamber 3 corresponding to the cover plate 12, and a clamping groove 34 is provided on the inner side of the upper side of the clamping plate 33, and a matching clamping block 14 is provided on the inner side of the lower end of the cover plate 12 corresponding to the clamping groove 34, and the tension strength of the tension spring 32 is greater than the torsion strength of the torsion spring 13, so that when the placement plate 31 is clamped with the cover plate 12, the tension spring 32 has enough force to pull the placement plate 31 to clamp the cover plate 12.
[0023] In another embodiment, see Figure 1 and 2 A thermometer and hygrometer 6 is also provided on the top of the box body 1. A temperature and humidity sensor 61 is provided on the top of the storage chamber 3 located at the top of the box body 1. The temperature and humidity sensor 61 is electrically connected to the thermometer and hygrometer 6, which can more accurately adjust the culture environment and ensure better growth of cells.
[0024] Working principle: An automated NK cell culture device, which delivers the same temperature and humidity to the culture area in each storage chamber 3 through a humidifier 5 and a flow cavity 41 of a partition 4, so as to avoid cell death due to environmental differences; when it is necessary to take out cells, it is only necessary to pull the placement tray 31 of the corresponding storage chamber 3, at which time the card block on the lower side of the cover plate 12 loses the card connection of the card slot 34, and the placement port 11 is opened under the action of the torsion spring 13, and the cell sample can be taken out by continuing to pull the placement tray 31, which is convenient and fast to take out, and at the same time avoids human hands from going deep into the storage chamber 3, and minimizes the pollution of the culture environment. When closing the cover, first close the cover 12, and then push the placement tray 31 in, so that the card slot 34 is engaged with the card block 14, and the tension of the tension spring 32 is used to fix the placement tray 31, so that it fixes and covers the cover 12, ensuring that the culture environment is consistent, and reducing the pollution of the culture environment by external air, thereby reducing the possibility of cell death due to pollution.
[0025] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.
[0026] In the description of the present invention, unless otherwise specified, "plurality" means two or more; the terms "upper", "lower", "left", "right", "inside", "outside", "front end", "rear end", "head", "tail", etc. indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation on the present invention.
[0027] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. An automated NK cell culture device, comprising a housing (1), characterized in that: The box body (1) is provided with multiple layers of storage cavities (2), each layer of the storage cavities (2) includes four storage chambers (3). A partition (4) is vertically provided in the middle of the box body (1), a flow chamber (41) is provided in the partition (4), and ventilation holes (42) are provided on the left and right side walls of the flow chamber (41) corresponding to the storage chambers (3) in each layer of the storage chamber (2). A humidifier (5) is provided on the upper side of the box body (1) corresponding to the flow chamber (41) of the partition (4), and the humidifier (5) is connected to the flow chamber (41). The side walls of the box body (1) are provided with placement openings (11) corresponding to the multiple storage chambers (3), and a cover plate (12) is hinged on the upper side of the placement opening (11), and a torsion spring (13) is provided at the hinge. A placement plate (31) is movably provided on the bottom side of the storage chamber (3). When the cover is closed, the outer side of the placement plate (31) is snap-fitted to the lower side of the cover plate (12).
2. The NK cell automated culture device according to claim 1, characterized in that: A tension spring (32) is provided at one end of the placement plate (31) facing the inner wall of the storage chamber (3); one end of the tension spring (32) is fixedly connected to the inner wall of the storage chamber (3); the other end is fixedly connected to one end of the placement plate (31) facing the inner wall of the storage chamber (3); and the placement plate (31) is slidably connected to the inner lower wall of the storage chamber (3).
3. The NK cell automated culture device according to claim 2, characterized in that: The tension strength of the tension spring (32) is greater than the torsion strength of the torsion spring (13).
4. The NK cell automated culture device according to claim 2, characterized in that: A clamping plate (33) is provided on the side of the placement plate (31) facing the outside of the storage chamber (3) corresponding to the cover plate (12); a clamping groove (34) is provided on the inner side of the upper side of the clamping plate (33); and a clamping block (14) matching the clamping groove (34) is provided on the inner side of the lower end of the cover plate (12) corresponding to the clamping groove (34).
5. The NK cell automated culture device according to claim 1, characterized in that: The top of the box (1) is also provided with a thermometer and hygrometer (6), and the top of the storage chamber (3) located at the top of the box (1) is provided with a temperature and humidity sensor (61), and the temperature and humidity sensor (61) is electrically connected to the thermometer and hygrometer (6).
6. The NK cell automated culture device according to claim 1, characterized in that: Transparent observation windows (15) are provided on the front and rear side walls of the box body (1) corresponding to each storage chamber (3).