Light supplementing device for tissue culture bottle and tissue culture bottle

Abstract

The utility model relates to a light supplementing device for a tissue culture bottle and the tissue culture bottle. The light supplementing device disclosed by the utility model is provided with the mounting hole which is detachably connected with the ventilation column on the bottle cap of the tissue culture bottle in a matching manner, and the light supplementing device is provided with the accommodating cavity which is filled with fluorescent powder. The light supplementing device is made of a transparent material and can absorb light energy when other exogenous light exists and emit light when no other exogenous light energy exists, so that light supplementing is carried out, other exogenous light can work at intervals, daily energy consumption of plant tissue culture is remarkably reduced, and tissue culture cost is reduced; the light supplementing device can provide required light for plants and promote the growth of the plants; the light supplementing device is simple in structure, low in production cost, suitable for an existing tissue culture bottle, convenient to assemble and disassemble and capable of being repeatedly used.

Description

Technical Field

[0001] The utility model relates to the technical field of tissue culture, in particular to a light-filling device for a tissue culture bottle and a tissue culture bottle. Background Art

[0002] Light is one of the most important external environmental conditions that affect plant growth and development. Its importance is not only reflected in plant photosynthesis, but light is also an important regulatory factor in the entire growth and development process of plants.

[0003] In the process of plant tissue culture, LED lamps are used to provide the plants with the required light. Different light qualities have a greater impact on tissue culture seedlings. Red light generally inhibits plant internode elongation, promotes tillering, and increases the accumulation of chlorophyll, carotenoids, soluble sugars and other substances. In the strawberry full-light experiment, it was found that red light is beneficial to increase the content of organic acids and total phenols in strawberries. Blue light can significantly shorten the internodes of plants, promote the lateral extension of plants, and reduce leaf area. At the same time, blue light can also promote the accumulation of plant secondary metabolites. Therefore, in plant tissue culture, different light qualities need to be provided as needed. The existing method is to use lamps with different light qualities and control the time by timing to supply the plant's demand for light. In the process of plant tissue culture, it is usually necessary to maintain 8-12 hours of light per day, and the power consumption is very large. Summary of the invention

[0004] The utility model aims to provide a novel light supplement device for a tissue culture bottle, which can supplement light when there is no other external light source.

[0005] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0006] The first aspect of the utility model provides a fill-light device for a tissue culture bottle, the fill-light device is provided with a mounting hole for detachably cooperating with a breathable column on a tissue culture bottle cap, the fill-light device is provided with a receiving cavity filled with fluorescent powder, and the fill-light device is made of a transparent material.

[0007] The fluorescent powder in the light-filling device of the utility model can absorb light energy when there is other external light (such as LED light), and continue to emit light when there is no other external light. Therefore, the light tube can be controlled to work intermittently. When the light tube is working, it absorbs light energy, and when the light tube is not working, the light-filling device performs light filling, thereby reducing the energy consumption of the light tube. The light-filling device is particularly suitable for nighttime light filling. In addition, by filling different colors of fluorescent powder, plants can be irradiated with different colors of light to promote plant growth.

[0008] The fill-light device of the utility model can be detachably installed on the bottle cap of the tissue culture bottle, preferably with an interference fit connection, and has good stability after assembly. Before use, the fill-light device can be directly put on the air permeable column of the bottle cap of the tissue culture bottle, or the air permeable column of the bottle cap of the tissue culture bottle can be inserted into the fill-light device. The fill-light device can be reused, and the fill-light device on the bottle cap of the tissue culture bottle can be selected to be installed or replaced when other colors of light are needed.

[0009] Furthermore, the light-filling device comprises an upper shell and a lower shell that are connected in a matching manner, the accommodating cavity is formed between the upper shell and the lower shell, and the upper shell is provided with an air hole for connecting the accommodating cavity with the outside world, so as to allow ventilation during the high-temperature and high-pressure sterilization of the tissue culture bottle, so as to avoid the phenomenon of bursting the device due to gas expansion during the sterilization process. The fluorescent powder in the light-filling device will not leak out of the air hole. Of course, in some embodiments, in order to further avoid the hidden danger of fluorescent powder leaking out, the fluorescent powder can be mixed with a viscous substance such as glue that does not affect the luminescence of the fluorescent powder and then applied or filled in the accommodating cavity.

[0010] Furthermore, the lower shell is provided with a mounting groove for detachably matingly connecting with the lower end portion of the upper shell.

[0011] Preferably, the lower end of the upper shell is connected to the mounting groove by interference fit, and has good stability after assembly.

[0012] According to some specific embodiments, the upper shell includes an integrally formed upper cover plate, a first outer peripheral wall and a first inner peripheral wall, a first through hole is provided in the center of the upper cover plate, the first outer peripheral wall extends downward from the outer periphery of the upper shell, the first inner peripheral wall extends downward from the upper cover plate along the circumference of the first through hole, and the lower ends of the first outer peripheral wall and the first inner peripheral wall are open, the lower shell includes an integrally formed lower cover plate, a second outer peripheral wall, a second inner peripheral wall and a third inner peripheral wall, a second through hole is provided in the center of the lower cover plate, the second outer peripheral wall extends upward from the outer periphery of the lower shell, the second inner peripheral wall extends upward from the lower shell along the circumference of the lower shell, the mounting groove is formed between the outer surface of the second inner peripheral wall and the inner surface of the second outer peripheral wall, the third inner peripheral wall extends upward from the lower shell along the circumference of the second through hole, and the upper ends of the second outer peripheral wall, the second inner peripheral wall and the third inner peripheral wall are open.

[0013] When the upper shell and the lower shell are matched and connected, the lower end of the first outer wall is located in the mounting groove, the lower end of the first inner wall is located in the through hole formed by the third inner wall, and the first through hole, the inner surface of the first inner wall and the second through hole form the mounting hole.

[0014] Furthermore, the first outer wall and the first inner wall are of equal length in the axial direction, the second outer wall, the second inner wall and the third inner wall are of equal length in the axial direction, and the length of the first outer wall and the first inner wall in the axial direction is 2 to 4 times the length of the second outer wall, the second inner wall and the third inner wall in the axial direction, thereby ensuring that the upper shell and the lower shell have a certain accommodating cavity for filling the phosphor after being stably matched and connected.

[0015] Furthermore, the inner diameters of the first through hole and the second through hole are equal, and there is a distance between the third inner wall and the edge of the second through hole, and the distance is the thickness of the first inner wall, so that the assembled fill light device has a compact and stable structure, which is convenient for inserting the air permeable column of the tissue culture bottle cap.

[0016] Specifically, the fluorescent powder is one or more of purple fluorescent powder, green fluorescent powder or red fluorescent powder. In practical application, a light-filling device filled with fluorescent powder of corresponding color can be selected according to actual needs.

[0017] Specifically, the fill light device is made of plastic.

[0018] Specifically, the upper shell and the lower shell can be integrally formed by 3D printing.

[0019] The second aspect of the utility model further provides a tissue culture bottle cover, which comprises a tissue culture bottle cover and the above-mentioned light-filling device, wherein the tissue culture bottle cover is provided with a ventilation column.

[0020] Furthermore, the light-filling device is cylindrical in shape as a whole, and its outer diameter is smaller than the inner diameter of the tissue culture bottle cap, and the axial length of the light-filling device is greater than or equal to the axial length of the air-permeable column.

[0021] The third aspect of the utility model further provides a tissue culture bottle, which comprises a bottle body, the above-mentioned supplementary light device and a tissue culture bottle cover, or comprises a bottle body and the above-mentioned tissue culture bottle cover.

[0022] Due to the application of the above technical solution, the utility model has the following advantages compared with the prior art:

[0023] The light-filling device of the utility model can absorb light energy when there is other external light energy, and emit light to provide light-filling when there is no other external light energy, so that other external light sources can work at intervals, significantly reducing the daily energy consumption of plant tissue culture and reducing the tissue culture cost; the light-filling device can provide the plants with the required light quality and promote plant growth; the light-filling device is not only simple in structure and low in production cost, but also suitable for existing tissue culture bottles, easy to load and unload, and can be reused. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1is a schematic diagram of the three-dimensional structure of the fill light device in Example 1;

[0025] Figure 2 is a top view of the fill light device in Example 1;

[0026] Figure 3 It is a bottom view of the fill light device in Example 1;

[0027] Figure 4 is a side view of the fill light device in Example 1;

[0028] Figure 5 is a cross-sectional view of the fill light device in Example 1;

[0029] Figure 6 is a schematic diagram of the three-dimensional structure of the fill light device in Example 1 at another viewing angle;

[0030] Figure 7 is a schematic diagram of the three-dimensional structure of the upper shell of the fill light device in Example 1;

[0031] Figure 8 It is a bottom view of the upper housing of the fill light device in Example 1;

[0032] Fig. 9 is a cross-sectional view of the upper housing of the fill light device in Example 1;

[0033] Fig.10 is a schematic diagram of the three-dimensional structure of the lower shell of the fill light device in Example 1;

[0034] Fig.11 is a schematic diagram of the three-dimensional structure of the lower housing of the fill light device in Example 1 from another viewing angle;

[0035] Fig.12 is a top view of the lower housing of the fill light device in Example 1;

[0036] Fig.13 is a cross-sectional view of the lower housing of the fill light device in Example 1;

[0037] Fig.14 It is a schematic diagram of the three-dimensional structure of the tissue culture bottle cap in Example 1;

[0038] Fig.15 This is a schematic diagram of the three-dimensional structure of the tissue culture bottle cap in Example 1 from another viewing angle;

[0039] Fig.16 is a schematic diagram of the three-dimensional structure of the bottle body in Example 1,

[0040] 11. Upper shell; 111. Upper cover; 112. First outer wall; 113. First inner wall; 1111. Air hole; 12. Lower shell; 121. Lower cover; 122. Second outer wall; 123. Second inner wall; 124. Third inner wall; 13. Mounting hole; 2. Tissue culture bottle cap; 21. Air column; 3. Bottle body. DETAILED DESCRIPTION

[0041] The utility model is further described below in conjunction with the embodiments shown in the accompanying drawings.

[0042] In the following, only some exemplary embodiments are briefly described. As those skilled in the art will appreciate, the described embodiments may be modified in various ways without departing from the spirit or scope of the embodiments of the present invention. Therefore, the drawings and descriptions are considered to be exemplary and non-restrictive in nature.

[0043] In the description of the present invention, it should be noted that the terms "upper" and "lower" are used in accordance with the Figure 1 , Figure 2 The status shown is defined. Figure 1 , Figure 2 In the figure, the side where the upper shell 11 is located is the "upper" side, and the side where the lower shell 12 is located is the "lower" side; "inside" and "outside" are positions defined by the distance relative to the center of the tissue culture bottle or component, wherein "inside" is a position close to the center of the tissue culture bottle or component, and "outside" is a position far from the center of the tissue culture bottle or component. The above-mentioned directional words are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the tissue culture bottle or component referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0044] The drawings provided in the present invention are merely schematic diagrams of tissue culture bottles for the convenience of readers' understanding and do not represent any limitation on the tissue culture bottles.

[0045] Example 1

[0046] like Figures 1 to 16As shown, this embodiment provides a tissue culture bottle, which includes a fill-in light device, a tissue culture bottle cap 2 and a bottle body 3. The fill-in light device is provided with a mounting hole 13 for detachably mating with the air-permeable column 21 on the tissue culture bottle cap 2, and the fill-in light device is provided with a receiving cavity, and the receiving cavity is filled with fluorescent powder. In this embodiment, the fill-in light device is cylindrical as a whole, and its outer diameter is smaller than the inner diameter of the tissue culture bottle cap 2, and the axial length of the fill-in light device is greater than or equal to the axial length of the air-permeable column of the tissue culture bottle cap 2. The fill-in light device includes an upper shell 11 and a lower shell 12 that are detachably matingly connected to each other, and a receiving cavity is formed between the upper shell 11 and the lower shell 12. The upper shell 11 includes an integrally formed upper cover plate 111, a first outer peripheral wall 112 and a first inner peripheral wall 113. A first through hole is provided in the center of the upper cover plate 111. The first outer peripheral wall 112 extends downward from the outer periphery of the upper shell 11. The first inner peripheral wall 113 extends downward from the upper cover plate 111 along the circumference of the first through hole. The lower ends of the first outer peripheral wall 112 and the first inner peripheral wall 113 are open. The lower shell 12 includes an integrally formed lower cover plate 121, a second peripheral wall 122, a second inner peripheral wall 123 and a third inner peripheral wall 124. A second through hole is provided in the center of the lower cover plate 121. The second peripheral wall 122 extends upward from the outer circumference of the lower shell 12. The second inner peripheral wall 123 extends upward from the lower shell 12 along the circumference of the lower shell 12. A mounting groove is formed between the outer surface of the second inner peripheral wall 123 and the inner surface of the second peripheral wall 122. The third inner peripheral wall 124 extends upward from the lower shell 12 along the circumference of the second through hole. The upper ends of the second peripheral wall 122, the second inner peripheral wall 123 and the third inner peripheral wall 124 are open. When the upper shell 11 and the lower shell 12 are matched and connected, the lower end of the first peripheral wall 112 is located in the mounting groove, the lower end of the first inner peripheral wall 113 is located in the through hole formed by the third inner peripheral wall 124, and the first through hole, the inner surface of the first inner peripheral wall 113 and the second through hole form a mounting hole 13. In this embodiment, the upper shell 11 and the lower shell 12 are connected by interference fit, and the structure is stable after assembly. In this embodiment, the first outer wall 112 and the first inner wall 113 are equal in length in the axial direction, the second outer wall 122, the second inner wall 123 and the third inner wall 124 are equal in length in the axial direction, and the length of the first outer wall 112 and the first inner wall 113 in the axial direction is 3 times the length of the second outer wall 122, the second inner wall 123 and the third inner wall 124 in the axial direction, so as to ensure that the upper shell 11 and the lower shell 12 have a certain accommodating cavity for filling the fluorescent powder after stable matching connection. The inner diameters of the first through hole and the second through hole are equal, and there is a distance between the third inner wall 124 and the edge of the second through hole, which is the thickness of the first inner wall 113, so that the structure of the light-filling device after assembly is compact and stable, and it is convenient for the air-permeable column 21 of the tissue culture bottle cap 2 to be inserted.In this embodiment, an air hole 1111 is provided on the upper cover plate 111. After the light-filling device is assembled, the air hole 1111 is used to connect the accommodating cavity with the outside world so as to facilitate ventilation during the high-temperature and high-pressure sterilization (121°C, 20 min) of the tissue culture device, so as to avoid the device from bursting due to gas expansion during the sterilization process.

[0047] The upper shell 11 and the lower shell 12 in this embodiment are both made of transparent plastic and are manufactured using 3D printing technology. The processing steps are as follows:

[0048] 1. Dimension measurement: First, accurately measure the relevant structural dimensions of the bottle mouth of the bottle body 3 and the inner cap 2 of the tissue culture bottle to ensure the accuracy of the data and provide basic data for subsequent modeling. This embodiment is aimed at the existing conventional 240mL tissue culture bottle.

[0049] 2.3D modeling: fusion360 software is used to perform accurate 3D modeling according to the measured dimensional data and the structural design of the upper shell 11 and the lower shell 12. During the modeling process, it is ensured that the structure and size of the model are consistent with the actual object and meet the use requirements. In this embodiment, the outer diameter of the first outer wall 112 of the upper shell 11 is 45 mm, the inner diameter of the first outer wall 112 is 41 mm, the outer diameter of the first inner wall 113 is 12 mm, the inner diameter of the first inner wall 113 (the inner diameter of the first through hole) is 8 mm, the aperture of the air vent 1111 is 2.5 mm, and the thickness of the upper cover 111 is 4 mm; the outer diameter of the second outer wall 122 of the lower shell 12 is 48 mm, the inner diameter of the second outer wall 122 is 45 mm, the outer diameter of the second inner wall 123 is 41 mm, the inner diameter of the second inner wall 123 is 38 mm, the outer diameter of the third inner wall 124 is 15 mm, the inner diameter of the third inner wall 124 is 12 mm, the inner diameter of the second through hole is 8 mm, and the thickness of the lower cover 121 is 2 mm.

[0050] 3. Model slicing: After modeling is completed, import the model file into the slicing software to slice the model. Slicing is an important step before 3D printing, which can convert the 3D model into a layered structure that the printer can recognize.

[0051] 4. Stereolithography 3D printing: Place the sliced ​​model file into the Stereolithography 3D printer and start printing. Stereolithography 3D printing technology can restore model details with high precision, ensuring that the printed parts meet the design requirements.

[0052] 5. Manual grinding: After printing is completed, take out the parts for manual grinding. The purpose of grinding is to remove burrs and uneven parts that may be generated during the printing process, making the surface of the parts smoother.

[0053] 6. Polishing: After grinding, the parts are polished. Polishing can further improve the surface finish of the parts and make them look more beautiful.

[0054] 7. Molding inspection: Check whether the size, structure and appearance of the parts meet the design requirements, install them on the bottle cap of a 240mL tissue culture bottle, test them, check their firmness, and conduct high temperature and high pressure tests (121℃, 20min) to ensure that the processing quality meets the standards.

[0055] Through the above steps, the entire processing process from measurement to forming can be completed. In this process, each link must be strictly controlled to ensure the quality and accuracy of the final product.

[0056] The fluorescent powder in this embodiment can be directly loaded into the receiving cavity. According to the designed size, the fluorescent powder will not leak out from the vent hole 1111. Of course, in some other embodiments, in order to further avoid the hidden danger of fluorescent powder leaking out, the fluorescent powder can be mixed with a viscous substance such as glue that does not affect the luminescence of the fluorescent powder and then applied or loaded into the receiving cavity. In this embodiment, blue fluorescent powder is loaded.

[0057] During the process of culturing tissue culture plants using the tissue culture bottle of this embodiment, the LED lamp can be adjusted to intermittent lighting. In the process of plant tissue culture, 8-12 hours of light are usually maintained every day. After adopting the tissue culture bottle of this embodiment, intermittent timed switch cycle lighting is performed at night, LED lighting is performed for half an hour, and the required red, blue, purple and other lighting is provided by fluorescence for half an hour. Therefore, after using the tissue culture bottle of this embodiment, the daily LED lighting is shortened by 4-6 hours, which not only saves electric energy, but also replenishes the red, blue, purple and other lighting needs of the plants through the supplementary light device, promotes plant growth, and the supplementary light device can be reused, and the production cost is low. The tissue culture bottle of this embodiment that can supplement blue light can be used in plant culture. The rose tissue culture seedlings inoculated in the culture medium grow well, with thick leaves and large growth.

[0058] The above embodiments are only for illustrating the technical concept and features of the utility model, and their purpose is to enable people familiar with the technology to understand the content of the utility model and implement it accordingly, and they cannot be used to limit the protection scope of the utility model. Any equivalent changes or modifications made according to the spirit of the utility model should be included in the protection scope of the utility model.

Claims

1. A light-filling device for a tissue culture bottle, characterized in that: The fill-light device is provided with a mounting hole (13) for detachably cooperating with a vent column on a tissue culture bottle cap, the fill-light device is provided with a receiving cavity filled with fluorescent powder, and the fill-light device is made of a transparent material.

2. The light-filling device for tissue culture bottles according to claim 1, characterized in that: The fill light device comprises an upper shell (11) and a lower shell (12) which are matched and connected to each other, the accommodating cavity is formed between the upper shell (11) and the lower shell (12), and the upper shell (11) is provided with a vent hole (1111) for connecting the accommodating cavity with the outside.

3. The light-filling device for tissue culture bottles according to claim 2, characterized in that: The lower shell (12) is provided with a mounting groove for detachably matingly connecting with the lower end of the upper shell (11).

4. The light-filling device for tissue culture bottles according to claim 3, characterized in that: The upper shell (11) comprises an integrally formed upper cover plate (111), a first outer peripheral wall (112) and a first inner peripheral wall (113); a first through hole is provided at the center of the upper cover plate (111); the first outer peripheral wall (112) extends downward from the outer periphery of the upper shell (11); the first inner peripheral wall (113) extends downward from the upper cover plate (111) along the circumference of the first through hole; the lower ends of the first outer peripheral wall (112) and the first inner peripheral wall (113) are open. The lower shell (12) comprises an integrally formed lower cover plate (121), a second outer peripheral wall (122), a second inner peripheral wall (123) and a third inner peripheral wall (124); a second through hole is provided at the center of the lower cover plate (121); the second outer peripheral wall (122) extends upward from the outer periphery of the lower shell (12); the second inner peripheral wall (123) extends upward from the lower shell (12) along the circumferential direction of the lower shell (12); the mounting groove is formed between the outer surface of the second inner peripheral wall (123) and the inner surface of the second outer peripheral wall (122); the third inner peripheral wall (124) extends upward from the lower shell (12) along the circumferential direction of the second through hole; the upper ends of the second outer peripheral wall (122), the second inner peripheral wall (123) and the third inner peripheral wall (124) are open; When the upper shell (11) and the lower shell (12) are matched and connected, the lower end of the first outer wall (112) is located in the installation groove, and the lower end of the first inner wall (113) is located in the through hole formed by the third inner wall (124), and the first through hole, the inner surface of the first inner wall (113) and the second through hole form the installation hole (13).

5. The light-filling device for tissue culture bottles according to claim 4, characterized in that: The first outer wall (112) and the first inner wall (113) have the same axial length, the second outer wall (122), the second inner wall (123) and the third inner wall (124) have the same axial length, and the first outer wall (112) and the first inner wall (113) have 2 to 4 times the axial length of the second outer wall (122), the second inner wall (123) and the third inner wall (124).

6. The light-filling device for tissue culture bottles according to claim 4, characterized in that: The inner diameters of the first through hole and the second through hole are equal, and there is a distance between the third inner surrounding wall (124) and the edge of the second through hole, the distance being the thickness of the first inner surrounding wall (113) in the radial direction.

7. The light-filling device for tissue culture bottles according to claim 1, characterized in that: The phosphor is one or more of purple phosphor, green phosphor or red phosphor; The material of the fill light device is plastic.

8. A tissue culture bottle cap, comprising a tissue culture bottle cap (2), wherein the tissue culture bottle cap (2) is provided with a vent column (21), characterized in that: The tissue culture bottle cover comprises the light supplement device according to any one of claims 1 to 7.

9. The tissue culture bottle cover according to claim 8, characterized in that: The light-filling device is cylindrical in shape as a whole, and its outer diameter is smaller than the inner diameter of the tissue culture bottle cap (2); the axial length of the light-filling device is greater than or equal to the axial length of the air-permeable column (21).

10. A tissue culture bottle, comprising a bottle body, characterized in that: The tissue culture bottle comprises the tissue culture bottle cover according to claim 8 or 9.

Images