Liquid nitrogen treatment device for biological sample

Abstract

The utility model discloses a liquid nitrogen treatment device for a biological sample, which comprises an outer barrel body, an inner barrel body and a liquid nitrogen treatment device, the inner cylinder body is movably arranged in the outer cylinder body, the inner cylinder body is used for placing a sample, a through hole is formed in the inner cylinder body, and liquid nitrogen contained in the outer cylinder body can flow into the inner cylinder body from the through hole; the cover body can be placed in the inner cylinder body and can be taken out from the inner cylinder body, the cover body comprises a floating cover, the floating cover can float on the liquid nitrogen, after the inner cylinder body is placed in the outer cylinder body, the liquid nitrogen enters the inner cylinder body through a through hole in the inner cylinder body, a sample in the inner cylinder body can be subjected to liquid nitrogen treatment, the floating cover floats on the liquid nitrogen, and when the sample makes contact with the liquid nitrogen and has a violent reaction, the liquid nitrogen is separated from the liquid nitrogen. According to the liquid nitrogen isolation device, the isolation effect can be achieved, liquid nitrogen is prevented from splashing, meanwhile, volatilization of the liquid nitrogen can be reduced, and the use safety is improved; after the preset liquid nitrogen treatment time is reached, the inner barrel body is lifted out of the outer barrel body, and all samples can be taken out at a time, so that the use is convenient, and the efficiency is high.

Description

Technical Field

[0001] The utility model relates to the technical field of medical equipment, in particular to a liquid nitrogen processing device for biological samples. Background Art

[0002] Liquid nitrogen is a substance formed by the liquefaction of nitrogen at extremely low temperatures. The temperature of liquid nitrogen is very low, about -196.56°C, which can provide excellent cooling effect. It is often used for long-term preservation of biological samples, such as cells, tissues, blood, sperm and eggs.

[0003] Clinically, during the extraction of extracellular matrix proteins, prokaryotic expressed proteins, etc., liquid nitrogen is required to cool the tissues or cells. The specific process is: immerse the tissue in liquid nitrogen, then centrifuge it, and repeat this process several times to fully break the cells.

[0004] However, there are currently no special tools to assist staff in performing the above operations. In the prior art, staff generally use a wine scoop to take out some liquid nitrogen from the laboratory and put it into a foam box or a thermos cup, and then put the sample in an EP tube (Eppendorf tube, i.e., centrifuge tube) into the taken out liquid nitrogen. After the processing time is reached, the EP tube is taken out with long tweezers, or the sample is taken out after the liquid is poured out.

[0005] The above device is not only relatively simple, inconvenient to use and inefficient, but also when the sample first contacts the liquid nitrogen surface, a violent reaction will occur due to the large temperature difference, causing liquid nitrogen to splash, causing frostbite or splashing sensitive parts such as the eyes; at the same time, since nitrogen expands rapidly when it vaporizes under normal pressure, large amounts of liquid nitrogen inhaled by workers after evaporation will affect their health. Therefore, using the above device to perform this operation has a high risk and low safety.

[0006] Therefore, the above-mentioned prior art has at least the following technical problems: the tools used in the prior art for treating biological samples with liquid nitrogen are inconvenient to use, have low efficiency, and low safety in use. Utility Model Content

[0007] The embodiment of the present application provides a liquid nitrogen treatment device for biological samples, thereby solving the technical problems in the prior art that tools used for liquid nitrogen treatment of biological samples are inconvenient to use, have low efficiency, and are less safe to use.

[0008] In order to solve the above technical problems, the embodiment of the present application provides a liquid nitrogen treatment device for samples, the device comprising:

[0009] An outer cylinder, which is used to contain liquid nitrogen;

[0010] An inner cylinder, movably disposed in the outer cylinder, the inner cylinder is used to place the sample, and a through hole is provided on the inner cylinder, and the liquid nitrogen contained in the outer cylinder can flow into the inner cylinder from the through hole, so that the liquid nitrogen acts on the sample;

[0011] The cover body can be placed in the inner cylinder and can be taken out from the inner cylinder. The cover body includes a floating cover, and the floating cover can float on liquid nitrogen.

[0012] Furthermore, the cover body also includes:

[0013] A pressing plate, wherein the density of the pressing plate is greater than that of liquid nitrogen, and is used for pressing the sample in the inner cylinder in the liquid nitrogen.

[0014] Furthermore, the pressing plate is hollow.

[0015] Furthermore, the cross-sectional shape of the pressing plate is the same as the cross-sectional shape of the inner cylinder.

[0016] Furthermore, the cover body comprises:

[0017] The rod body, the pressing plate is fixed at the bottom end of the rod body, and the floating cover is located above the pressing plate and movably sleeved on the rod body to float on the liquid nitrogen.

[0018] Furthermore, the diameter of the floating cover is smaller than the inner diameter of the inner cylinder.

[0019] Furthermore, the edge of the opening of the inner cylinder extends outward to form a skirt, and the skirt is used to partially cover the gap between the outer cylinder and the inner cylinder.

[0020] Furthermore, an outer cylinder handle is provided on one side of the outer cylinder, an inner cylinder handle is provided at the cylinder mouth of the inner cylinder, and a cover handle is constructed on the top end of the rod body;

[0021] Wherein, the height of the outer cylinder handle is adjustable.

[0022] Furthermore, the outer cylinder handle, the inner cylinder handle and the cover handle are all constructed with insulation structures to reduce the transfer of cold from liquid nitrogen to the outer cylinder handle, the inner cylinder handle and the cover handle.

[0023] Furthermore, the through holes are arranged on the wall surface and the bottom surface of the inner cylinder.

[0024] One or more technical solutions provided in the embodiments of the present application have at least the following technical effects or advantages:

[0025] In the embodiment of the present application, a dedicated outer cylinder is provided to take and hold an appropriate amount of liquid nitrogen, and an inner cylinder is provided to uniformly place the samples. After the inner cylinder is placed in the outer cylinder, the liquid nitrogen enters the inner cylinder through the through hole on the inner cylinder, and the sample in the inner cylinder can be treated with liquid nitrogen. In addition, in the embodiment of the present application, a cover is provided on the inner cylinder, wherein the floating cover can float on the liquid nitrogen, and when the sample contacts the liquid nitrogen and reacts violently, it can play an isolating role to prevent the liquid nitrogen from splashing.

[0026] At the same time, the floating cover can also reduce the volatilization of liquid nitrogen and its contact with air, thereby improving the safety of use;

[0027] In addition, after the predetermined liquid nitrogen treatment time is reached, the inner cylinder can be taken out from the outer cylinder to take out all samples at one time, which is convenient to use and highly efficient.

[0028] In addition, the cover body also includes a pressing plate, the density of which is greater than that of liquid nitrogen, and the sample in the inner cylinder can be pressed into the liquid nitrogen to prevent the sample from floating on the liquid nitrogen and affecting the cooling effect of the sample.

[0029] In summary, the liquid nitrogen treatment device for biological samples described in the embodiments of the present application effectively solves the technical problems in the prior art that the tools used for liquid nitrogen treatment of biological samples are inconvenient to use, have low efficiency, and have low safety in use. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0031] Figure 1 This is a schematic structural diagram of a liquid nitrogen treatment device for biological samples in one embodiment of the utility model;

[0032] Figure 2 This is a structural cross-sectional view of a liquid nitrogen treatment device for biological samples in one embodiment of the utility model;

[0033] Figure 3 The structure explosion of a liquid nitrogen treatment device for biological samples in one embodiment of the utility model Figure 1 ;

[0034] Figure 4 The structure explosion of a liquid nitrogen treatment device for biological samples in one embodiment of the utility model Figure 2 ;

[0035] Figure 5The structure of the outer cylinder in one embodiment of the utility model is shown in FIG. Figure 1 ;

[0036] Figure 6 The structure of the outer cylinder in one embodiment of the utility model is shown in FIG. Figure 2 ;

[0037] Figure 7 It is a side view of the outer cylinder in one embodiment of the utility model;

[0038] Figure 8 The structure of the inner cylinder in one embodiment of the utility model is shown in FIG. Figure 1 ;

[0039] Fig. 9 The structure of the inner cylinder in one embodiment of the utility model is shown in FIG. Figure 2 ;

[0040] Fig.10 It is a side view of the inner cylinder in one embodiment of the utility model;

[0041] Fig.11 The structure of the cover body in one embodiment of the utility model is shown in FIG. Figure 1 ;

[0042] Fig.12 The structure of the cover body in one embodiment of the utility model is shown in FIG. Figure 2 ;

[0043] Fig.13 It is a side view of a cover body in one embodiment of the utility model;

[0044] Fig.14 It is a schematic structural diagram of a pressing plate in one embodiment of the utility model. DETAILED DESCRIPTION

[0045] The embodiment of the present application provides a liquid nitrogen treatment device for biological samples, thereby solving the technical problems in the prior art that tools used for liquid nitrogen treatment of biological samples are inconvenient to use, have low efficiency, and are less safe to use.

[0046] In order to better understand the above technical solution, the above technical solution will be described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0047] like Figures 1 to 4 As shown, one or more embodiments of the present application provide a liquid nitrogen treatment device for biological samples, the device comprising:

[0048] The outer cylinder 100 is used for taking and containing liquid nitrogen;

[0049] The inner cylinder 200 is movably disposed in the outer cylinder 100 so as to be taken out of the outer cylinder 100. The inner cylinder 200 is used to place the sample, and a through hole 220 is provided on the inner cylinder 200. The liquid nitrogen contained in the outer cylinder 100 can flow into the inner cylinder 200 from the through hole 220 so that the liquid nitrogen acts on the sample.

[0050] The cover body 300 can be placed in the inner cylinder body 200 and can be taken out from the inner cylinder body 200. The cover body 300 includes a floating cover 310, and the floating cover can float on liquid nitrogen.

[0051] As can be seen from the above description, the embodiment of the present application provides a dedicated outer cylinder 100 to take and hold an appropriate amount of liquid nitrogen, and provides an inner cylinder 200 to uniformly place samples. After the inner cylinder 200 is placed in the outer cylinder 100, the liquid nitrogen enters the inner cylinder 200 through the through hole 220 on the inner cylinder 200, and the sample in the inner cylinder 200 can be treated with liquid nitrogen. In addition, the embodiment of the present application also provides a cover 300 on the inner cylinder 200, wherein the floating cover 310 can float on the liquid nitrogen. When the sample contacts the liquid nitrogen and reacts violently, it can play an isolating role to avoid liquid nitrogen splashing. At the same time, it can also reduce the volatilization of liquid nitrogen and contact with air, thereby improving the safety of use. After the predetermined liquid nitrogen treatment time is reached, the inner cylinder 200 is taken out from the outer cylinder 100, and all samples can be taken out at one time. It is convenient to use and efficient, and effectively solves the technical problems of inconvenient use, low efficiency, and low safety of use of tools used for liquid nitrogen treatment of biological samples in the prior art.

[0052] It should be noted that the sample refers to biological samples such as cells, tissues, blood, sperm and eggs, and the sample is placed in the inner cylinder 200 by being placed in a sample carrier. For example, when the sample is a tissue or cell in the extraction process of extracellular matrix proteins, prokaryotic expression proteins, etc., the sample carrier is a centrifuge tube.

[0053] In addition, the outer cylinder 100 and the inner cylinder 200 can be made of stainless steel, which exhibits good corrosion resistance and high strength in liquid nitrogen. Figures 5 to 10 As shown, the outer cylinder 100 and the inner cylinder 200 may be in the shape of a hollow cylinder with an opening at the top, or may be in other shapes, such as a hollow cuboid with an opening at the top, as long as an inner cavity for containing liquid nitrogen and for placing samples can be formed, and there is no limitation here.

[0054] In addition, the density of the floating cover 310 needs to be less than that of liquid nitrogen. For example, the floating cover 310 can be made of a foam material, and the foam material can be polystyrene (PS) or polyurethane (PU).

[0055] In one embodiment of the present application, Figures 11 to 13 As shown, the cover body 300 also includes:

[0056] The pressing plate 320 may be placed in the inner cylinder 200 , and the density of the pressing plate 320 is greater than that of liquid nitrogen, so as to press the sample in the inner cylinder 200 in the liquid nitrogen.

[0057] Specifically, at standard atmospheric pressure (101.325 kPa) and room temperature, the density of liquid nitrogen is approximately 0.808 g / cm 3 In order to enable the pressing plate 320 to press the sample downward and prevent the sample from floating on the liquid nitrogen and affecting the cooling effect of the sample, the pressing plate 320 can be made of stainless steel (density is about 8.00g / cm 3 ) or other materials with a density greater than the liquid nitrogen. Of course, it can also be made of other suitable materials and is not limited here.

[0058] In one embodiment of the present application, Fig.14 As shown, the pressing plate 320 is hollowed out to control the overall weight of the pressing plate 320 .

[0059] Further, still Fig.14 As shown, the cross-sectional shape of the pressing plate 320 is the same as that of the inner cylinder 200 so as to press down all samples in the inner cylinder 200 as much as possible. For example, the cross-sectional shape of the pressing plate 320 and that of the inner cylinder 200 are both circular.

[0060] In one embodiment of the present application, Figures 11 to 13 As shown, the cover body 300 includes:

[0061] The rod body 330, the pressing plate 320 is coaxially fixed to the bottom end of the rod body 330, the floating cover 310 is coaxially movably sleeved on the rod body 330 to float on the liquid nitrogen, and the top end of the rod body 330 constitutes a cover handle.

[0062] Specifically, by holding the cover handle, the cover 300 can be placed in the inner cylinder 200 or taken out from the inner cylinder 200, which is very convenient.

[0063] In one embodiment of the present application, the diameter of the floating cover 310 is smaller than the inner diameter of the inner cylinder 200, which can facilitate the floating cover 310 to float above the liquid nitrogen in the inner cylinder 200 to partially cover the liquid nitrogen surface and reduce the volatilization of liquid nitrogen. It can also prevent the floating cover 310 from completely covering the surface of the liquid nitrogen, avoiding the formation of a closed space, resulting in explosion after excessive nitrogen accumulation, thereby improving safety in use.

[0064] In one embodiment of the present application, Figures 8 to 10 As shown, the edge of the opening of the inner cylinder 200 extends outward to form a skirt 230, and the skirt 230 is used to partially cover the gap between the outer cylinder 100 and the inner cylinder 200 to reduce the volatilization of liquid nitrogen in the gap.

[0065] In one embodiment of the present application, Figures 1 to 10 As shown, an outer cylinder handle 110 is provided on one side of the outer cylinder 100, and an inner cylinder handle 210 is provided at the cylinder mouth of the inner cylinder 200 to facilitate hand holding and improve convenience of use.

[0066] Specifically, by holding the outer cylinder handle 110, it is convenient to directly use the outer cylinder 100 to take out liquid nitrogen from the liquid nitrogen tank, similar to a scoop. The inner cylinder handle 210 is arranged at the mouth of the inner cylinder 200 to prevent the inner cylinder handle 210 from entering the outer cylinder 100, and the depth of the inner cylinder 200 inserted into the outer cylinder 100 can be controlled by controlling the depth of the inner cylinder 200, without affecting the use.

[0067] Furthermore, the height of the outer cylinder handle 110 is adjustable, so when the outer cylinder 100 is used to take liquid nitrogen from a laboratory liquid nitrogen tank, the outer cylinder handle 110 can be adjusted to a suitable height to adapt to liquid nitrogen at different liquid levels.

[0068] For example, Figure 7 As shown, the outer cylinder handle 110 includes a horizontal transverse handle 112 (extending along the radial direction of the outer cylinder 100) and a vertical handle 113 (extending along the axial direction of the outer cylinder 100), wherein the vertical handle 113 has a telescopic joint, so that the height of the outer cylinder handle 110 can be adjusted.

[0069] Furthermore, the outer cylinder handle 110, the inner cylinder handle 210 and the cover handle are all constructed with an insulating structure to reduce the transfer of cold from the liquid nitrogen to the outer cylinder handle 110, the inner cylinder handle 210 and the cover handle, thereby avoiding discomfort in holding due to too low a temperature.

[0070] For example, the thermal insulation structure may be that the outer cylinder handle 110, the inner cylinder handle 210 and the cover handle are all made of materials with poor thermal conductivity, such as wood, ceramics, polymer plastics (such as polyethylene PE, polyvinyl chloride PVC), etc.

[0071] Furthermore, the ends of the outer cylinder handle 110, the inner cylinder handle 210 and the cover handle are all provided with expanded parts 111, 211, 340 for easy hand holding, which can not only avoid slipping from the hand and improve the holding stability, but also the expanded parts 111, 211, 340 are made of materials with poor thermal conductivity, which can further reduce the cold transfer from liquid nitrogen and improve the comfort of use; in addition, the diameter of the expanded part 340 on the rod body 330 is larger than the rod body 330, which can also prevent the floating cover 310 from slipping from the rod body 330.

[0072] Furthermore, the inner cylinder handle 210 is foldable. For example, the inner cylinder handle 210 includes at least two parts hinged to each other to reduce space occupation after folding.

[0073] In one embodiment of the present application, the through holes 220 are evenly distributed on the wall surface and the bottom surface of the inner cylinder 200 to improve the efficiency of liquid nitrogen entering the inner cylinder 200 and the cooling efficiency of the sample.

[0074] An exemplary method of using a liquid nitrogen treatment device for biological samples provided in an embodiment of the present application is as follows:

[0075] Use the outer cylinder 100 to take out liquid nitrogen from the liquid nitrogen tank (you can use the outer cylinder 100 directly to take it out, or you can use other containers to take it out and then pour it into the outer cylinder 100), put the sample to be processed into the EP tube, and record the label, put the EP tube with the sample into the inner cylinder 200, then put the cover 300 into the inner cylinder 200, hold the inner cylinder handle 210, and put the inner cylinder 200 into the outer cylinder 100; when the processing time is reached, hold the inner cylinder handle 210, take out the inner cylinder 200, and take out the cover 300 from the inner cylinder 200, and then pour out the sample.

[0076] It should be understood that although the terms "first", "second", etc. may be used herein to describe various units, these units should not be limited by these terms. These terms are used only to distinguish one unit from another. For example, a first unit may be referred to as a second unit, and similarly, a second unit may be referred to as a first unit without departing from the scope of the exemplary embodiments.

[0077] The directional terms such as outer, middle, inner, etc. mentioned or may be mentioned in this specification are defined relative to the structures shown in the drawings. They are relative concepts and may change accordingly according to different positions and different usage conditions. Therefore, these or other directional terms should not be interpreted as restrictive terms.

[0078] The above is only a preferred embodiment of the present application, and is not any formal or substantial limitation to the present application. It should be pointed out that ordinary technicians in this technical field can make several improvements and supplements without departing from the method of the present application, and these improvements and supplements should also be regarded as the protection scope of the present utility model. Any technician familiar with this profession can make some changes, modifications and evolutions of the technical content disclosed above without departing from the spirit and scope of the present application, which are equivalent embodiments of the present application; at the same time, any changes, modifications and evolutions of any equivalent changes made to the above embodiments based on the substantial technology of the present application are still within the scope of the technical solution of the present application.

Claims

1. A liquid nitrogen treatment device for biological samples, characterized in that: The device comprises: An outer cylinder, which is used to contain liquid nitrogen; An inner cylinder, movably disposed in the outer cylinder, the inner cylinder is used to place the sample, and a through hole is provided on the inner cylinder, and the liquid nitrogen contained in the outer cylinder can flow into the inner cylinder from the through hole, so that the liquid nitrogen acts on the sample; The cover body can be placed in the inner cylinder and can be taken out from the inner cylinder. The cover body includes a floating cover, and the floating cover can float on liquid nitrogen.

2. A liquid nitrogen treatment device for biological samples as claimed in claim 1, characterized in that: The cover body also includes: A pressing plate, wherein the density of the pressing plate is greater than that of liquid nitrogen, and is used for pressing the sample in the inner cylinder in the liquid nitrogen.

3. A liquid nitrogen treatment device for biological samples as claimed in claim 2, characterized in that: The pressing plate is hollow.

4. A liquid nitrogen treatment device for biological samples as claimed in claim 2, characterized in that: The cross-sectional shape of the pressing plate is the same as the cross-sectional shape of the inner cylinder.

5. The liquid nitrogen treatment device for biological samples according to claim 2, characterized in that: The cover body comprises: The rod body, the pressing plate is fixed at the bottom end of the rod body, and the floating cover is located above the pressing plate and movably sleeved on the rod body to float on the liquid nitrogen.

6. The liquid nitrogen treatment device for biological samples according to claim 1, characterized in that: The diameter of the floating cover is smaller than the inner diameter of the inner cylinder.

7. The liquid nitrogen treatment device for biological samples according to claim 1, characterized in that: The edge of the opening of the inner cylinder extends outward to form a skirt, and the skirt is used to partially cover the gap between the outer cylinder and the inner cylinder.

8. The liquid nitrogen treatment device for biological samples according to claim 5, characterized in that: An outer cylinder handle is provided on one side of the outer cylinder, an inner cylinder handle is provided at the cylinder mouth of the inner cylinder, and a cover handle is constructed on the top end of the rod body; Wherein, the height of the outer cylinder handle is adjustable.

9. A liquid nitrogen treatment device for biological samples as claimed in claim 8, characterized in that: The outer cylinder handle, the inner cylinder handle and the cover handle are all constructed with heat insulation structures to reduce the cold transferred from the liquid nitrogen to the outer cylinder handle, the inner cylinder handle and the cover handle.

10. The liquid nitrogen treatment device for biological samples according to claim 1, characterized in that: The through holes are evenly arranged on the wall surface and the bottom surface of the inner cylinder.

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