Sterilization device for plant tissue culture

Abstract

The utility model belongs to the technical field of tissue culture, and discloses a sterilization device for plant tissue culture, which comprises a box body structure and a sterilization assembly, the box body structure comprises a sterilization box, the sterilization assembly comprises a protection box and an infusion pump, the protection box is arranged at the top of the sterilization box, and the infusion pump is arranged in the protection box. The input end of the liquid conveying pump is connected with a liquid inlet pipe, the output end of the liquid conveying pump is connected with an output pipe, a plurality of connecting pipes are installed on one side of the output pipe, valves are installed on one sides of the connecting pipes, one ends of the connecting pipes are connected with flow dividing pipes, a plurality of branch pipes are installed on one sides of the flow dividing pipes, and a plurality of spraying heads are installed at the bottoms of the branch pipes. A high-temperature sterilization assembly is installed in the sterilization box and comprises a protection frame. The plant tissue culture sterilization device has the advantages that sterilization liquid is sprayed on the surface of plant tissue culture, bacteria, fungi or viruses are prevented from breeding on the surface of the plant tissue, the interior of the sterilization device is sterilized at high temperature, and the safety of plant tissue culture is improved.

Description

Technical Field

[0001] The utility model relates to a sterilizing device, in particular to a sterilizing device for plant tissue culture, belonging to the technical field of plant tissue culture. Background Art

[0002] Plant tissue culture is a new asexual reproduction technology developed in recent decades based on the theory that plant cells are totipotent. Plant tissue culture is also called in vitro culture in a broad sense, which refers to the technology of separating tissues, organs or cells, protoplasts, etc. that meet the needs from the plant body, inoculating them on a culture medium containing various nutrients and plant hormones under sterile conditions to obtain regenerated complete plants or produce other products with economic value through aseptic operation. In a narrow sense, it refers to the use of various parts of plant tissues, such as cambium, parenchyma, mesophyll tissue, endosperm, etc. to obtain regenerated plants. It also refers to the culture of callus tissue produced from various organs during the culture process, and the callus tissue is then redifferentiated to form a regenerated plant. Bacteria, fungi or viruses will be produced during plant tissue culture, and plant tissue culture needs to be sterilized.

[0003] Chinese patent number CN220654331U provides an explant sterilization device for plant tissue culture, including a disinfection bottle, a disinfection structure and a filtering structure; an internal thread segment structure is provided on the upper periphery of the disinfection bottle, the disinfection structure is provided with a first external thread segment structure, and the filtering structure is provided with a second external thread segment structure; the disinfection structure is detachably mounted on the disinfection bottle by cooperating with the first external thread segment structure and the internal thread segment structure, or the filtering structure is detachably mounted on the disinfection bottle by cooperating with the second external thread segment structure and the internal thread segment structure; the disinfection structure is provided with a first filter screen structure for pressing the explant into the disinfectant, and the filtering structure is provided with a pouring spout connected to the disinfection bottle.

[0004] However, although the above case can fully mix the explant and the disinfectant by turning it upside down; when pouring out the disinfectant, the pouring spout can prevent the disinfectant from dripping onto the work platform, but the sterilizing liquid cannot be sprayed on the surface of the plant tissue culture, which may easily cause bacteria, fungi or viruses to grow on the surface of the plant tissue. At the same time, the inside of the sterilization device cannot be disinfected at high temperature, resulting in relatively poor safety of the plant tissue culture.

[0005] In view of this, the present utility model is proposed. Utility Model Content

[0006] The purpose of the utility model is to provide a sterilizing device for plant tissue culture in order to solve the above problems.

[0007] The utility model achieves the above-mentioned purpose through the following technical solutions:

[0008] A sterilization device for plant tissue culture, comprising a box structure and a sterilization component, the box structure comprising a sterilization box, the sterilization component comprising a protection box and an infusion pump, the protection box being installed on the top of the sterilization box, the infusion pump being installed inside the protection box, the input end of the infusion pump being connected to a liquid inlet pipe, the output end of the infusion pump being connected to an output pipe, a plurality of connecting pipes being installed on one side of the output pipe, a valve being installed on one side of the connecting pipe, one end of the connecting pipe being connected to a shunt pipe, a plurality of branch pipes being installed on one side of the shunt pipe, a plurality of spray heads being installed on the bottom of the branch pipe, a high-temperature sterilization component being installed in the sterilization box, the high-temperature sterilization component comprising a protection frame being installed on the inner wall of the sterilization box, and an electric heating pipe being embedded and installed on one side of the protection frame.

[0009] Furthermore, a distributor is installed on the top of the sterilization box, and the distributor is connected to the electric heating tube through a conductive wire.

[0010] Furthermore, a box door is installed on the front of the sterilization box through a hinge, and an observation window is embedded in the front of the box door.

[0011] Furthermore, a partition is installed inside the sterilization box, and a stop bar is installed on the top of the partition.

[0012] Furthermore, a bottom plate is installed at the bottom of the sterilization box, and supporting legs are installed at the bottom of the bottom plate.

[0013] Furthermore, a liquid storage assembly is installed on the top of the sterilization box, and the liquid storage assembly includes a liquid storage box. The liquid storage box is installed on the top of the sterilization box, and the free end of the liquid inlet pipe is located in the liquid storage box.

[0014] Furthermore, a liquid level meter is embedded in the front of the liquid storage tank, and a filter is installed inside the liquid storage tank.

[0015] Furthermore, a liquid inlet hopper is installed on the top of the liquid storage tank, and a threaded cover is threadedly connected to the outside of the top of the liquid inlet hopper.

[0016] The technical effects and advantages of the utility model are as follows: (1) Through the sterilization component, the infusion pump in the sterilization component can transport the sterilization liquid in the liquid storage tank to the sterilization box, and the sterilization liquid is sprayed on the plant tissue through the liquid inlet pipe, the outlet pipe, the connecting pipe, the shunt pipe, the branch pipe and the spray head. The sterilization liquid can be sprayed on the surface of the plant tissue culture to prevent the growth of bacteria, fungi or viruses on the surface of the plant tissue. The valve is used for easy individual control, which improves the convenience of fixed-point control sterilization. (2) Through the high-temperature sterilization component, the electric heating tube in the high-temperature sterilization component can perform high-temperature disinfection on the inside of the sterilization device, thereby improving the safety of plant tissue culture. (3) Through the liquid storage component, the liquid level gauge in the liquid storage component can observe the liquid of the sterilization liquid in the liquid storage tank, and the filter net used can enter the sterilization liquid in the liquid storage tank for filtration. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0018] Figure 2 It is a schematic diagram of the internal structure of the utility model;

[0019] Figure 3 This is a schematic diagram of the structure of the infusion pump of the utility model;

[0020] Figure 4 This is a schematic diagram of the structure of the sterilization component of the utility model.

[0021] In the figure: 100, box structure; 101, sterilization box; 102, box door; 103, observation window; 104, partition; 105, stop bar; 106, bottom plate; 107, support leg; 200, liquid storage component; 201, liquid storage box; 202, liquid level meter; 203, filter screen; 204, liquid inlet bucket; 205, threaded cover; 300, sterilization component; 301, protection box; 302, infusion pump; 303, liquid inlet pipe; 304, output pipe; 305, connecting pipe; 306, valve; 307, shunt pipe; 308, branch pipe; 309, spray head; 400, high-temperature sterilization component; 401, distributor; 402, protection frame; 403, electric heating tube. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0023] See also Figure 1-4As shown, a sterilization device for plant tissue culture includes a box structure 100 and a sterilization component 300. The box structure 100 includes a sterilization box 101. The sterilization component 300 includes a protection box 301 and an infusion pump 302. The infusion pump 302 transports the sterilization liquid in the liquid storage box 201 to the sterilization box 101. The protection box 301 is installed on the top of the sterilization box 101. The infusion pump 302 is installed inside the protection box 301. The input end of the infusion pump 302 is connected to a liquid inlet pipe 303, and the output end of the infusion pump 302 is connected to an output pipe 304. A plurality of connecting pipes 303 are installed on one side of the output pipe 304. 5. A valve 306 is installed on one side of the connecting pipe 305. The valve 306 is used for individual control, which improves the convenience of fixed-point control sterilization. A shunt pipe 307 is connected to one end of the connecting pipe 305. A plurality of branch pipes 308 are installed on one side of the shunt pipe 307. A plurality of spray heads 309 are installed at the bottom of the branch pipe 308. The sterilizing liquid is sprayed on the plant tissue through the liquid inlet pipe 303, the output pipe 304, the connecting pipe 305, the shunt pipe 307, the branch pipe 308 and the spray head 309. The sterilizing liquid is sprayed on the surface of the plant tissue culture to prevent bacteria, fungi or viruses from growing on the surface of the plant tissue.

[0024] A high-temperature sterilization component 400 is installed in the sterilization box 101. The high-temperature sterilization component 400 includes a protective frame 402 and a distributor 401. The distributor 401 supplies power to the electric heating tube 403. The distributor 401 is installed on the top of the sterilization box 101. The distributor 401 is connected to the electric heating tube 403 through a conductive wire. The protective frame 402 is installed on the inner wall of the sterilization box 101. The electric heating tube 403 is embedded in one side of the protective frame 402. The electric heating tube 403 performs high-temperature disinfection on the inside of the sterilization device to improve the safety of plant tissue culture sterilization.

[0025] Specifically, Figure 1 As shown, a door 102 is installed on the front of the sterilization box 101 through a hinge, and an observation window 103 is embedded in the front of the door 102. The observation window 103 is used to observe the interior of the sterilization box 101. A partition 104 is installed inside the sterilization box 101, and a stop bar 105 is installed on the top of the partition 104. The stop bar 105 can block the sterilization liquid and plant tissue. A bottom plate 106 is installed at the bottom of the sterilization box 101, and a support leg 107 is installed at the bottom of the bottom plate 106. The support leg 107 can support the sterilization device.

[0026] Specifically, Figure 2 , 3As shown, a liquid storage component 200 is installed on the top of the sterilization box 101, and the liquid storage component 200 includes a liquid storage box 201, which is installed on the top of the sterilization box 101. The free end of the liquid inlet pipe 303 is located in the liquid storage box 201, and a liquid level gauge 202 is embedded in the front of the liquid storage box 201. The liquid level gauge 202 observes the liquid of the sterilizing liquid in the liquid storage box 201. A filter net 203 is installed inside the liquid storage box 201. The filter net 203 is used to filter the sterilizing liquid in the liquid storage box 201. A liquid inlet hopper 204 is installed on the top of the liquid storage box 201. The liquid inlet hopper 204 is used to facilitate the sterilizing liquid to enter the liquid storage box 201. The top of the liquid inlet hopper 204 is externally threaded with a threaded cover 205.

[0027] Working principle:

[0028] When in use, place the sterilizing device at the designated position, then open the threaded cover 205, inject the sterilizing liquid into the liquid storage tank 201, observe the liquid of the sterilizing liquid in the liquid storage tank 201 through the liquid level meter 202, and at the same time, use the filter net 203 to filter the sterilizing liquid in the liquid storage tank 201, then open the box door 102, put the plant component on the partition 104, and then close the box door 102, start the infusion pump 302, and the infusion pump 302 will transport the sterilizing liquid in the liquid storage tank 201 to the sterilization box 101, and the sterilizing liquid will pass through the liquid inlet pipe 303, the outlet pipe 304, and the connecting pipe 306. 305, the shunt pipe 307, the branch pipe 308 and the spray head 309 are sprayed on the plant tissue, and the sterilizing liquid is sprayed on the surface of the plant tissue culture to prevent the growth of bacteria, fungi or viruses on the surface of the plant tissue. The valve 306 is used to facilitate individual control and improve the convenience of fixed-point control sterilization. After sterilization, the plant components are taken out, and then the sterilization box 101 is sterilized at high temperature, and the distributor 401 is started. The distributor 401 supplies power to the electric heating tube 403, and the electric heating tube 403 performs high-temperature disinfection on the inside of the sterilization device to improve the safety of plant tissue culture.

[0029] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.

[0030] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A sterilization device for plant tissue culture, comprising a box structure (100) and a sterilization component (300), characterized in that: The box structure (100) comprises a sterilization box (101), and the sterilization assembly (300) comprises a protection box (301) and an infusion pump (302), wherein the protection box (301) is installed on the top of the sterilization box (101), and the infusion pump (302) is installed inside the protection box (301), the input end of the infusion pump (302) is connected to a liquid inlet pipe (303), and the output end of the infusion pump (302) is connected to an output pipe (304), and a plurality of connecting pipes (305) are installed on one side of the output pipe (304), and the connecting pipes (305) are connected to the infusion pipe (303). A valve (306) is installed on one side, one end of the connecting pipe (305) is connected to a shunt pipe (307), a plurality of branch pipes (308) are installed on one side of the shunt pipe (307), a plurality of spray heads (309) are installed at the bottom of the branch pipes (308), a high-temperature sterilization component (400) is installed in the sterilization box (101), and the high-temperature sterilization component (400) comprises a protective frame (402), the protective frame (402) is installed on the inner wall of the sterilization box (101), and an electric heating pipe (403) is embedded and installed on one side of the protective frame (402).

2. A sterilizing device for plant tissue culture according to claim 1, characterized in that: A distributor (401) is installed on the top of the sterilization box (101), and the distributor (401) is connected to the electric heating tube (403) via a conductive wire.

3. A sterilizing device for plant tissue culture according to claim 1, characterized in that: A box door (102) is installed on the front of the sterilization box (101) via a hinge, and an observation window (103) is embedded and installed on the front of the box door (102).

4. A sterilizing device for plant tissue culture according to claim 1, characterized in that: A partition (104) is installed inside the sterilization box (101), and a stop bar (105) is installed on the top of the partition (104).

5. A sterilizing device for plant tissue culture according to claim 1, characterized in that: A bottom plate (106) is installed at the bottom of the sterilization box (101), and a supporting leg (107) is installed at the bottom of the bottom plate (106).

6. A sterilizing device for plant tissue culture according to claim 1, characterized in that: A liquid storage assembly (200) is installed on the top of the sterilization box (101). The liquid storage assembly (200) comprises a liquid storage box (201). The liquid storage box (201) is installed on the top of the sterilization box (101). The free end of the liquid inlet pipe (303) is located inside the liquid storage box (201).

7. A sterilizing device for plant tissue culture according to claim 6, characterized in that: A liquid level meter (202) is embedded and installed on the front side of the liquid storage tank (201), and a filter screen (203) is installed inside the liquid storage tank (201).

8. A sterilizing device for plant tissue culture according to claim 6, characterized in that: A liquid inlet hopper (204) is installed on the top of the liquid storage box (201), and a threaded cover (205) is threadedly connected to the outside of the top of the liquid inlet hopper (204).

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