Liquid changing device for animal cell culture

Abstract

The utility model discloses a liquid changing device for animal cell culture, and relates to the technical field of cell culture. Comprising a liquid changing box, one side of the liquid changing box is fixedly connected with a first hinge, the liquid changing box is rotatably connected with a transparent box door through the first hinge, a culture bottle is arranged in the liquid changing box, the top of the liquid changing box is fixedly connected with a fixing cylinder in a penetrating mode, a liquid spraying mechanism is arranged in the fixing cylinder, and the inner wall of the top end of the fixing cylinder is rotatably connected with a fixing ring; the inner side of the fixing ring is fixedly connected with a rotating plate, the top of the rotating plate is slidably connected with a flow-dividing liquid injection pipe and a liquid suction pipe in a penetrating mode, and the bottom ends of the flow-dividing liquid injection pipe and the liquid suction pipe are fixedly connected with movable plates in a penetrating mode. The problems that extraneous strains are easy to adhere to the culture medium for changing the liquid, so that the experimental result is deviated, or the cell culture fails are solved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a liquid replacement device for animal cell culture. Background Art

[0002] With the development of science and technology, animal cell culture has gradually attracted the attention of biologists. Animal cell culture can provide scientific basis and scientific results for new drug screening, vaccines, genetically engineered drugs, cell engineering drugs, monoclonal antibodies, and drug research and development.

[0003] When changing the fluid in existing animal cell cultures, the operation needs to be performed on a sterile table. During the process of changing the fluid, the door of the sterile table is always open. As a result, foreign bacteria are easily attached to the culture medium during the process of changing the fluid, which may cause deviations in the experimental results or cell culture failure. Therefore, a fluid changing device for animal cell culture is needed. Utility Model Content

[0004] The purpose of the utility model is to provide a liquid replacement device for animal cell culture, which solves the above-mentioned technical problems.

[0005] In order to achieve the above-mentioned purpose, the utility model provides the following technical solution: a fluid replacement device for animal cell culture, comprising.

[0006] A fluid exchange box, one side of which is fixedly connected with a hinge 1, and the fluid exchange box is rotatably connected with a transparent box door through the hinge 1, a culture bottle is arranged inside the fluid exchange box, a fixed cylinder is fixedly connected through the top of the fluid exchange box, a liquid spraying mechanism is arranged inside the fixed cylinder, a fixed ring is rotatably connected to the inner wall of the top of the fixed cylinder, a rotating plate is fixedly connected to the inner side of the fixed ring, a shunt injection tube and a liquid suction tube are slidably connected through the top of the rotating plate, and the bottom ends of the shunt injection tube and the liquid suction tube are both fixedly connected A movable plate is connected, and sliding blocks are fixedly connected to the sides of the two movable plates. A rotating groove and a limiting groove are opened on the inner wall of the fixed cylinder, and the rotating groove and the limiting groove are interconnected. The side of the sliding block is rotatably connected to the inner wall of the rotating groove, and the side of the sliding block is slidably connected to the inner wall of the limiting groove. The top of the shunt injection tube is fixedly connected to the shunt delivery tube, and the bottom end of the shunt delivery tube is provided with a filling mechanism. The top of the suction tube is fixedly connected to the guide tube, and the bottom end of the guide tube is provided with a recovery mechanism.

[0007] Preferably, the liquid spraying mechanism comprises a water pump and a liquid storage tank, a liquid filling tank is provided inside the fixed cylinder, an atomizing nozzle is passed through and fixedly connected to the inner wall of the fixed cylinder, the atomizing nozzle is communicated with the liquid filling tank, one end of the water pump passes through and is fixedly connected to the side of the fixed cylinder, the other end of the water pump passes through and is fixedly connected to one side of the liquid storage tank, an inlet pipe is passed through and fixedly connected to the top of the liquid storage tank, a pipe plug is movably inserted into the inner wall of the inlet pipe, and the bottom of the liquid storage tank is fixedly connected to the top of the liquid replacement tank.

[0008] Preferably, the liquid filling mechanism includes water pump 2 and water pump 4, the two ends of the bottom side of the diversion delivery pipe are fixedly connected to the top of water pump 2 and water pump 4, the bottom ends of water pump 2 and water pump 4 are both passed through and fixedly connected to the top of the liquid tank, the bottoms of water pump 2 and water pump 4 are both fixedly connected to hoses, heating plates are fixedly connected to both sides of the inner wall of the liquid tank, hinge 2 is fixedly connected to one side of the liquid tank, and the liquid tank is rotatably connected to a box door via hinge 2.

[0009] Preferably, the recovery mechanism includes a water pump three and a liquid collecting tank, the bottom end of the guide tube is fixedly connected to the top end of the water pump three, and the bottom end of the water pump three passes through and is fixedly connected to the top of the liquid collecting tank.

[0010] Preferably, the bottom of the inner wall of the liquid exchange box is fixedly connected to a fixing platform, and the bottom end of the culture bottle is movably plugged into the inner wall of the groove at the top of the fixing platform.

[0011] Preferably, a lead-out pipe is fixedly connected through a side of the liquid collecting box away from the liquid exchange box, and a second pipe plug is movably inserted into the inner wall of an end of the lead-out pipe away from the liquid collecting box.

[0012] Compared with the related art, the liquid replacement device for animal cell culture provided by the utility model has the following beneficial effects:

[0013] 1. The utility model provides a fluid replacement device for animal cell culture. By providing a shunt injection tube and a pipette, the problem of not exposing the culture bottle to an exposed fluid replacement environment for a long time is solved, and the fluid in the culture bottle can be replaced, rinsed and supplemented with new base fluid, thereby greatly reducing the possibility of foreign bacteria attachment, thereby improving the accuracy of data and the success rate of the experiment.

[0014] 2. The utility model provides a fluid exchange device for animal cell culture. By providing a fluid storage tank, it solves the problem that before or at the end of use of the shunt injection tube and the pipette, in order to ensure the sterility of the bottom ends of the shunt injection tube and the pipette, the bottom ends of the shunt injection tube and the pipette can be sprayed and disinfected by an additional water pump and an atomizing nozzle. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1It is a schematic diagram of the overall structure of the utility model;

[0016] Figure 2 It is a schematic cross-sectional diagram of the overall structure of the utility model;

[0017] Figure 3 This is a schematic diagram of the structure disassembly of the fixed cylinder of the utility model;

[0018] Figure 4 This is a schematic diagram of the side splitting of the overall structure of the utility model rotated 120 degrees.

[0019] In the figure: 1. shunt delivery pipe; 2. shunt injection pipe; 3. pipe plug one; 4. inlet pipe; 5. water pump one; 6. liquid storage tank; 7. liquid exchange tank; 8. water pump two; 9. liquid placement tank; 10. box door; 11. guide pipe; 12. suction tube; 13. rotating plate; 14. water pump three; 15. liquid collecting tank; 16. fixed cylinder; 17. transparent box door; 18. heating plate; 19. hose; 20. culture bottle; 21. fixed table; 22. limit groove; 23. atomizing nozzle; 24. limit ring; 25. fixed ring; 26. movable plate; 27. sliding block; 28. outlet pipe; 29. ​​pipe plug two; 30. water pump four. DETAILED DESCRIPTION

[0020] Embodiment 1:

[0021] See also Figure 1-Figure 4 The utility model provides a technical solution, including a liquid exchange box 7, one side of the liquid exchange box 7 is fixedly connected with a hinge 1, the liquid exchange box 7 is rotatably connected with a transparent box door 17 through the hinge 1, a culture bottle 20 is arranged inside the liquid exchange box 7, a fixed cylinder 16 is fixedly connected through the top of the liquid exchange box 7, a liquid spraying mechanism is arranged inside the fixed cylinder 16, a fixed ring 25 is rotatably connected to the inner wall of the top of the fixed cylinder 16, a rotating plate 13 is fixedly connected to the inner side of the fixed ring 25, a shunt injection tube 2 and a liquid suction tube 12 are slidably connected to the top of the rotating plate 13, and the shunt injection tube 2 and the liquid suction tube 12 are slidably connected. 2 are all penetrated and fixedly connected with movable plates 26 at the bottom ends, and the sides of the two movable plates 26 are fixedly connected with sliding blocks 27, the inner wall of the fixed cylinder 16 is provided with a rotating groove and a limiting groove 22, the rotating groove and the limiting groove 22 are interlinked, the side of the sliding block 27 is rotatably connected to the inner wall of the rotating groove, and the side of the sliding block 27 is slidably connected to the inner wall of the limiting groove 22, the top of the shunt injection tube 2 is fixedly connected with the shunt delivery tube 1, and the bottom end of the shunt delivery tube 1 is provided with a filling mechanism, the top of the suction tube 12 is fixedly connected with the guide tube 11, and the bottom end of the guide tube 11 is provided with a recovery mechanism;

[0022] The filling mechanism includes a water pump 2 8 and a water pump 4 30, the two ends of the bottom side of the shunt delivery pipe 1 are fixedly connected to the top of the water pump 2 8 and the water pump 4 30, the bottom ends of the water pump 2 8 and the water pump 4 30 are both fixedly connected to the top of the liquid tank 9, the bottoms of the water pump 2 8 and the water pump 4 30 are both fixedly connected to the hose 19, the heating plates 18 are fixedly connected to both sides of the inner wall of the liquid tank 9, the hinge 2 is fixedly connected to one side of the liquid tank 9, and the liquid tank 9 is rotatably connected to the box door 10 through the hinge 2;

[0023] The recovery mechanism includes a water pump 3 14 and a liquid collecting tank 15. The bottom end of the guide pipe 11 is fixedly connected to the top end of the water pump 3 14. The bottom end of the water pump 3 14 penetrates and is fixedly connected to the top of the liquid collecting tank 15.

[0024] The bottom of the inner wall of the liquid exchange box 7 is fixedly connected with a fixing table 21, and the bottom end of the culture bottle 20 is movably plugged into the inner wall of the top groove of the fixing table 21;

[0025] A lead-out pipe 28 is fixedly connected to the side of the liquid collecting box 15 away from the liquid changing box 7 , and a pipe plug 29 is movably inserted into the inner wall of the end of the lead-out pipe 28 away from the liquid collecting box 15 .

[0026] In this embodiment: first, the box door 10 is opened by rotating the hinge 2, and then the hoses 19 at the bottom ends of the water pump 2 8 and the water pump 4 30 are respectively inserted into the culture medium liquid bottle and the PBS liquid bottle, and then the heating plate 18 is started to preheat the culture medium liquid bottle and the PBS liquid bottle. After preheating, the top of the pipette 12 is held and the movable plate 26 at the bottom end of the pipette 12 is rotated so that the sliding block 27 on the side of the movable plate 26 at the bottom end of the pipette 12 corresponds to the limit groove 22. At this time, the rotating plate 13 is rotated accordingly through the fixing ring 25, and the transparent box is closed by the hinge 1. The door 17 is rotated to open, and then the culture bottle 20 after disinfection and opening the cover is inserted into the groove on the top of the fixed platform 21 for fixing operation. At this time, the pipette 12 is pushed downward so that the bottom end of the pipette 12 goes deep into the culture bottle 20, and then the water pump 3 14 is started to perform the liquid suction operation. At this time, the original culture liquid in the culture bottle 20 is sucked out. After the absorption is completed, the water pump 3 14 is stopped, and then the bottom end of the pipette 12 is drawn out of the culture bottle 20. At this time, the sliding block 27 on the side of the movable plate 26 at the bottom end of the pipette 12 extends into the rotating groove inside the fixed cylinder 16;

[0027] Then rotate the pipette 12 again so that the sliding block 27 on the side of the movable plate 26 at the bottom end of the shunt injection tube 2 corresponds to the limiting groove 22, so as to hold the top of the shunt injection tube 2 and push the bottom end of the shunt injection tube 2 into the culture bottle 20. At this time, start the water pump 4 30, and the water pump 4 30 injects PBS liquid into the culture bottle 20 through the hose 19. After the injection, first extend the bottom end of the shunt injection tube 2 out of the interior of the culture bottle 20, and then rotate to open the transparent box door 17. The staff gently shakes the PBS liquid in the culture bottle 20 to perform a rinsing operation. The rinsed culture bottle 20 continues to be placed on the fixed table 21 in the liquid exchange box 7, and the transparent box door 17 is rotated to close, and then the above steps are repeated again. The rotating plate 13 is rotated 180 degrees to push the bottom end of the pipette 12 into the culture bottle 20 to suck out the PBS liquid in the culture bottle 20. After sucking out, the pipette 12 is reset, and then the rotating plate 13 is rotated 180 degrees to push the bottom end of the shunt injection tube 2 into the culture bottle 20. At this time, the water pump 28 is started to inject new culture medium into the culture bottle 20 through the water pump 28. At this time, the liquid replacement operation is completed. When the shunt injection tube 2 and the pipette 12 complete the liquid replacement operation, the shunt injection tube 2 and the pipette 12 need to be rotated 90 degrees so that the sliding block 27 leaves the limiting groove 22, so that the shunt injection tube 2 or the pipette 12 will not slide downward;

[0028] The limiting ring 24 on the inner wall of the fixed tube 16 can prevent the shunt injection tube 2 or the suction tube 12 from continuing to fall downward when the staff fails to reset the shunt injection tube 2 or the suction tube 12, thereby causing damage to the bottom end of the shunt injection tube 2 or the suction tube 12.

[0029] Embodiment 2:

[0030] On the basis of embodiment 1, the utility model provides a technical solution: the liquid spraying mechanism includes a water pump 5 and a liquid storage tank 6, a liquid filling tank is opened inside the fixed cylinder 16, an atomizing nozzle 23 is fixedly connected to the inner wall of the fixed cylinder 16, the atomizing nozzle 23 is connected to the liquid filling tank, one end of the water pump 5 is fixedly connected to the side of the fixed cylinder 16, the other end of the water pump 5 is fixedly connected to one side of the liquid storage tank 6, the top of the liquid storage tank 6 is fixedly connected to an inlet pipe 4, the inner wall of the inlet pipe 4 is movably connected to a pipe plug 3, and the bottom of the liquid storage tank 6 is fixedly connected to the top of the liquid replacement tank 7.

[0031] In the present embodiment: before or after the use of the shunt injection tube 2 and the pipette 12, in order to ensure the sterility of the bottom ends of the shunt injection tube 2 and the pipette 12, the pipe plug 3 can be removed from the top end of the introduction tube 4, and then disinfecting alcohol is injected into the liquid storage tank 6 through the introduction tube 4. After the injection, the pipe plug 3 is reinserted into the introduction tube 4 for sealing. When the shunt injection tube 2 and the pipette 12 need to be disinfected, the water pump 5 is started at this time, and the water pump 5 sucks the alcohol in the liquid storage tank 6 into the filling bin in the fixed cylinder 16, and then the bottom ends of the shunt injection tube 2 and the pipette 12 can be sprayed and disinfected through the atomizing nozzle 23.

[0032] Working principle: first start the spray mechanism to disinfect the shunt injection tube 2 and the pipette 12, then insert the hoses 19 at the bottom of the water pump 2 8 and the water pump 4 30 into the culture medium bottle and the PBS bottle respectively, start the heating plate 18 for preheating, after preheating, insert the sterilized and opened culture bottle 20 into the groove at the top of the fixed table 21 for fixing, then hold the top of the pipette 12 and push the pipette 12 downward so that the bottom end of the pipette 12 goes deep into the culture bottle 20, and the original culture liquid in the culture bottle 20 is sucked out by the water pump 3 14. After the absorption is completed, reset the pipette 12, and then rotate the pipette 12 so that the sliding block 27 on the side of the movable plate 26 at the bottom end of the shunt injection tube 2 corresponds to the limit groove 22, thereby holding it. The top of the shunt injection tube 2 is used to push the bottom end of the shunt injection tube 2 into the culture bottle 20, and PBS liquid is first injected into the culture bottle 20 through the water pump 24 30 and the hose 19. Then the staff rotates to open the transparent box door 17, gently shakes the PBS liquid in the culture bottle 20, and performs a rinsing operation. After rinsing, the culture bottle 20 is continued to be placed on the fixed table 21 in the liquid replacement box 7, and the transparent box door 17 is rotated to close. Then, the above steps are repeated to push the bottom end of the pipette 12 into the culture bottle 20 for aspiration. After aspiration, the pipette 12 is reset, and then the rotating plate 13 is rotated 180 degrees to push the bottom end of the shunt injection tube 2 into the culture bottle 20, and new culture medium liquid is injected into the culture bottle 20 through the water pump 28.

Claims

1. A fluid exchange device for animal cell culture, comprising a fluid exchange box (7), characterized in that: A hinge 1 is fixedly connected to one side of the fluid exchange box (7), and the fluid exchange box (7) is rotatably connected to a transparent box door (17) via the hinge 1. A culture bottle (20) is arranged inside the fluid exchange box (7). A fixed cylinder (16) is fixedly connected to the top of the fluid exchange box (7), and a liquid spraying mechanism is arranged inside the fixed cylinder (16). A fixed ring (25) is rotatably connected to the inner wall of the top of the fixed cylinder (16), and a rotating plate (13) is fixedly connected to the inner side of the fixed ring (25). A shunt injection tube (2) and a liquid suction tube (12) are slidably connected to the top of the rotating plate (13), and the bottom ends of the shunt injection tube (2) and the liquid suction tube (12) are both fixedly connected to the fixed cylinder (16). A movable plate (26) is connected, and the sides of the two movable plates (26) are fixedly connected with sliding blocks (27). The inner wall of the fixed cylinder (16) is provided with a rotation groove and a limit groove (22), and the rotation groove and the limit groove (22) are connected to each other. The side of the sliding block (27) is rotationally connected to the inner wall of the rotation groove, and the side of the sliding block (27) is slidably connected to the inner wall of the limit groove (22). The top of the diversion injection tube (2) is fixedly connected with the diversion delivery tube (1), and the bottom end of the diversion delivery tube (1) is provided with a filling mechanism. The top of the suction tube (12) is fixedly connected with the guide tube (11), and the bottom end of the guide tube (11) is provided with a recovery mechanism.

2. The liquid replacement device for animal cell culture according to claim 1, characterized in that: The liquid spraying mechanism comprises a water pump (5) and a liquid storage tank (6); a liquid filling tank is provided inside the fixed cylinder (16); an atomizing nozzle (23) is fixedly connected to the inner wall of the fixed cylinder (16); the atomizing nozzle (23) is connected to the liquid filling tank; one end of the water pump (5) is fixedly connected to the side of the fixed cylinder (16); the other end of the water pump (5) is fixedly connected to one side of the liquid storage tank (6); an inlet pipe (4) is fixedly connected to the top of the liquid storage tank (6); a pipe plug (3) is movably inserted into the inner wall of the inlet pipe (4); and the bottom of the liquid storage tank (6) is fixedly connected to the top of the liquid replacement tank (7).

3. The liquid replacement device for animal cell culture according to claim 1, characterized in that: The liquid filling mechanism comprises a water pump 2 (8) and a water pump 4 (30), the two ends of the bottom side of the diversion delivery pipe (1) are fixedly connected to the top of the water pump 2 (8) and the water pump 4 (30), the bottom ends of the water pump 2 (8) and the water pump 4 (30) are both passed through and fixedly connected to the top of the liquid tank (9), the bottoms of the water pump 2 (8) and the water pump 4 (30) are both fixedly connected to a hose (19), the inner walls of the liquid tank (9) are both fixedly connected to a heating plate (18), one side of the liquid tank (9) is fixedly connected to a hinge 2, and the liquid tank (9) is rotatably connected to a box door (10) via the hinge 2.

4. The liquid replacement device for animal cell culture according to claim 1, characterized in that: The recovery mechanism comprises a water pump three (14) and a liquid collecting tank (15), the bottom end of the guide pipe (11) is fixedly connected to the top end of the water pump three (14), and the bottom end of the water pump three (14) passes through and is fixedly connected to the top of the liquid collecting tank (15).

5. The fluid replacement device for animal cell culture according to claim 1, characterized in that: The bottom of the inner wall of the liquid exchange box (7) is fixedly connected to a fixing platform (21), and the bottom end of the culture bottle (20) is movably plugged into the inner wall of the top groove of the fixing platform (21).

6. The liquid replacement device for animal cell culture according to claim 4, characterized in that: A lead-out pipe (28) is fixedly connected to and penetrates the side of the liquid collecting box (15) away from the liquid exchange box (7), and a second pipe plug (29) is movably inserted into the inner wall of the end of the lead-out pipe (28) away from the liquid collecting box (15).

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