Miniature low-flux full-automatic chemiluminescence immunoassay analyzer

Abstract

The utility model discloses a miniature low-flux full-automatic chemiluminescence immunoassay analyzer which comprises a sample system, a filling station, a washing system and an incubation detection system which are sequentially arranged in the same straight line direction, the washing system is provided with a rotatable washing tray, a cup receiving groove is integrated on the washing tray, a cup discharging system is arranged on one side of the washing system, and a cup feeding system is arranged on the other side of the washing system. The cup arranging system can be used for arranging the reaction cups to the cup receiving groove one by one; the incubation detection system integrates incubation and detection functions; the gripper system is arranged above the linear direction, the reagent system is arranged on one side of the sample system, the rotary lifting type filling system is arranged on the side, close to the washing system, between the reagent system and the sample system, and in the rotating process of the rotary lifting type filling system, a filling needle of the rotary lifting type filling system can sweep over the reagent system, the sample system and a filling position. The utility model has the advantages of compact layout, good economical efficiency and low cost, and can meet the detection requirements of low flux and miniaturization of the analyzer.

Description

Technical Field

[0001] The utility model relates to the technical field of medical detection, in particular to a miniature low-throughput fully automatic chemiluminescence immunoassay analyzer. Background Art

[0002] Chemiluminescent immunoassay is a new type of labeled immunoassay technology that combines chemiluminescence or bioluminescence systems with immune reactions to detect trace amounts of antigens or antibodies. Because luminescent immunoassay has ultra-trace detection capabilities and high sensitivity, it is currently recognized as the most accurate and mature detection method for tumor markers and various hormones.

[0003] In order to avoid the influence of uncertain factors such as cumbersome operations, long sample turnover cycle and human interference in traditional biomedical testing, chemiluminescence immunoassay equipment is also developing in the direction of full automation, that is, an integrated analyzer that can realize filling, shaking, dilution, incubation, washing and detection.

[0004] The fully automatic chemiluminescence immunoassay analyzers currently available on the market are usually large in size and suitable for large-scale testing. However, with the continuous development of technology and different testing needs, the demand for low-throughput, miniaturized fully automatic chemiluminescence immunoassay analyzers is also increasing. However, the functional modules and overall layout of the current analyzer are designed based on the technical requirements of high-throughput testing. Therefore, the comprehensive layout and functional integration of the current modules cannot adapt to the low-throughput, miniaturized design of the analyzer. Utility Model Content

[0005] In view of this, the utility model provides a miniature low-throughput fully automatic chemiluminescence immunoassay analyzer, which can meet the low-throughput and miniaturized detection requirements of the analyzer through reasonable layout and design.

[0006] To achieve the above purpose, the technical solution of the utility model is as follows:

[0007] A miniature low-throughput fully automatic chemiluminescence immunoassay analyzer, the key of which is: comprising a sample system, a filling position, a washing system and an incubation detection system arranged in sequence in the same straight line direction, wherein the washing system has a rotatable washing tray, the washing tray is integrated with a cup receiving groove, a cup row system is arranged on one side of the washing system, and the cup row system can discharge reaction cups one by one into the cup receiving groove; the incubation detection system integrates incubation and detection functions;

[0008] A gripper system is arranged above the straight line direction, and the gripper system can transfer the reaction cup between the cup receiving tank, the filling position, the washing system and the incubation detection system at will;

[0009] A reagent system is arranged on one side of the sample system, and a rotary lifting filling system is arranged on the side between the reagent system and the sample system close to the washing system. During the rotation of the rotary lifting filling system, its filling needle can sweep over the reagent system, the sample system and the filling position.

[0010] The above structure has the advantages of compact layout, good economy and low cost, and can meet the low-throughput and miniaturized detection requirements of the analyzer.

[0011] Preferably, the sample system comprises a base, a carrier rotatably mounted on the base, and a second driving assembly for driving the carrier to rotate, wherein loading stations are distributed on the circumferential side of the carrier, and a universal connecting structure is provided on the loading station, and the universal connecting structure is used for detachably mounting a sample rack or a TIP head rack, wherein a dilution station is provided between two adjacent loading stations;

[0012] At least one set of positioning structures is integrated on the loading station, and the positioning structures are used to position and install liquid consumable bottles.

[0013] Preferably, the device further comprises a pipette, which is arranged above the sample system and is used to grab the TIP head in the sample system to transfer liquid.

[0014] Preferably, the carrier includes a column structure and a support plate extending radially outward from the lower part of the column structure, the loading station circular array is distributed on the circumferential side of the column structure, the dilution station includes a support frame arranged on the upper part of the column structure and extending outward, and the support frame is provided with a second cup placement hole for placing a reaction cup.

[0015] As a preference, it also includes a mixing mechanism arranged in the linear direction, the mixing mechanism includes a base plate, a rotating seat rotatably mounted on the base plate, and a third driving assembly for driving the rotating seat to rotate, a cup holder is rotatably mounted on the rotating seat, a cup placement groove for placing a reaction cup is provided on the upper part of the cup holder, a guide column is fixed on the base plate, a restraint groove is provided on one side of the lower part of the cup holder, and the restraint groove is movably sleeved on the guide column;

[0016] The rotation centerline of the cup seat is eccentrically arranged relative to the rotation centerline of the rotating seat. Under the restraining action of the restraining groove and the guide column, when the third driving assembly drives the rotating seat to rotate, the cup seat has both revolution and small oscillation along its rotation axis.

[0017] Preferably, the washing system comprises a first base and a first driving assembly, the washing tray is rotatably mounted in the first base, the first driving assembly is used to drive the washing tray to rotate, the washing tray is provided with first cup placement holes distributed in a circular array, each of the first cup placement holes is arranged in a vertical direction, the washing tray is provided with a magnetic assembly at a position close to the bottom of each of the first cup placement holes, and the magnetic assembly can rotate synchronously with the washing tray during the rotation of the washing tray relative to the first base;

[0018] The upper part of the washing tray is provided with a flange extending outwards, and cup placement notches are evenly distributed on the flange, and the cup placement notches constitute the cup receiving groove.

[0019] Preferably, the reagent system comprises a reagent compartment, a refrigeration module and a fourth drive assembly, wherein a reagent tray for storing reagents is rotatably mounted in the reagent compartment, and the fourth drive assembly is used to drive the reagent tray to rotate;

[0020] A first assembly notch is provided on the side of the reagent chamber, and the refrigeration module includes a semiconductor refrigeration element and a cold circulation fan, wherein the semiconductor refrigeration element has a hot side and a cold side, the semiconductor refrigeration element is installed at the first assembly notch position, and the cold side is located inside the reagent chamber, the hot side is located outside the reagent chamber, and the cold circulation fan is installed on the cold side.

[0021] Preferably, the reagent tray is provided with a clamping structure distributed along the circumference, and a reagent rack assembly is detachably placed on the clamping structure;

[0022] The reagent rack assembly includes a basic reagent rack, which is provided with three basic mounting holes distributed in a linear direction, each of which extends in the height direction of the basic reagent rack, and an extended reagent rack is detachably installed on one side of the basic reagent rack, and the extended reagent rack is provided with an extended mounting hole extending in the height direction, and the extended mounting hole and the three basic mounting holes are all used to place reagent bottles.

[0023] Preferably, the incubation detection system comprises a cabin and an incubation tray rotatably arranged inside the cabin, a fifth driving assembly for driving the incubation tray to rotate is arranged at the bottom of the cabin, the incubation tray is constructed as a cylindrical structure, and is provided with a plurality of circles of incubation holes distributed in a circular array, wherein at least one of the incubation holes located in the outermost circle is defined as a detection hole position, a detection window is arranged on the side of the incubation tray, which is directly opposite to and connected to the lower part of the detection hole position, and the detection window exposes the lower part of the reaction cup located in the detection hole position;

[0024] A second assembly notch is provided on the side of the cabin body, and a detection module is fixedly installed at the position of the second assembly notch. The light emitting detection element of the detection module faces the interior of the cabin body and is arranged at the same height as the detection window.

[0025] Preferably, the rotary lifting filling system comprises a second base, the second base is provided with a column which can rotate and slide up and down relative to the second base, the top of the column is provided with a cantilever, and the filling needle is arranged at the far end of the cantilever;

[0026] The second base is provided with a lifting drive assembly for driving the column to slide up and down, and a rotating drive assembly for driving the column to rotate, the second base comprises a first vertical plate and a second vertical plate arranged in a vertical direction, a supporting bottom plate is welded to the lower ends of the first vertical plate and the second vertical plate, and a mounting top plate is welded to the upper ends, the first vertical plate, the second vertical plate, the supporting bottom plate and the mounting top plate are constructed into a cubic structure, and an assembly space is enclosed therein;

[0027] The lower part of the column is arranged in the assembly space so as to be slidable up and down, the lifting drive assembly is arranged on the second vertical plate, and the rotating drive assembly is arranged on the mounting top plate.

[0028] Compared with the prior art, the beneficial effects of the utility model are:

[0029] 1. The miniature low-throughput fully automatic chemiluminescence immunoassay analyzer provided by the utility model can make the internal structure of the analyzer compact by reasonably arranging various functional modules in the space, integrating the transfer cup function in the washing system, and integrating the incubation and detection functions in the incubation and detection system, so as to meet the requirements of low-throughput and miniaturization of the analyzer, and has the advantages of good economy and low cost.

[0030] 2. With the miniature low-throughput fully automatic chemiluminescence immunoassay analyzer provided by the utility model, the sample rack, TIP head rack and liquid consumables bottle can be integrated and assembled around the carrier. The user can select the TIP head filling function according to whether the test requires dilution. At the same time, it does not take up additional space. It has the advantages of reasonable and compact layout, high functional integration and strong practicality.

[0031] 3. By directly installing the semiconductor refrigeration element of the refrigeration module on the first assembly notch on the side of the reagent compartment, and the cold side of the semiconductor refrigeration element is located inside the reagent compartment, the cold side can be directly opposite the side of the reagent bottle on the reagent tray, so that the cold capacity of the semiconductor refrigeration element can be directly applied to the reagent bottle. As the reagent tray rotates, the reagents in each reagent bottle can be fully cooled, meeting the insulation and refrigeration requirements of the reagents in the reagent compartment. Such a structural design is not only simple and compact, but also does not occupy additional height space, further ensuring the compact and miniaturized design of the analyzer. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 This is a schematic diagram of the overall layout of a micro low-throughput fully automatic chemiluminescence immunoassay analyzer;

[0033] Figure 2 A three-dimensional cross-sectional view showing the internal structure of a miniature low-throughput fully automated chemiluminescence immunoassay analyzer;

[0034] Figure 3 A three-dimensional cross-sectional view showing the internal structure of the washing system C;

[0035] Figure 4 is a schematic diagram of the structure of the washing system C;

[0036] Figure 5 is a cross-sectional view of the washing system C;

[0037] Figure 6 is a cross-sectional view of the washing tray 1 (to show the layout of the first cup placement hole 1b, the cup receiving groove 1a and the magnetic component 3);

[0038] Figure 7 It is a structural diagram of sample system A;

[0039] Figure 8 This is a reference diagram of the usage status of sample system A;

[0040] Fig. 9 is a cross-sectional view of the sample system A in use;

[0041] Fig.10 is a structural schematic diagram of the mixing mechanism J;

[0042] Fig.11 is a cross-sectional view of the mixing mechanism J;

[0043] Fig.12 It is a schematic diagram of the structure of the reagent system G;

[0044] Fig.13 is a cross-sectional view of the reagent system G;

[0045] Fig.14 is another cross-sectional view of the reagent system G;

[0046] Fig.15 A three-dimensional cross-sectional view showing the internal structure of the reagent system G;

[0047] Fig.16 It is a structural schematic diagram of the refrigeration module G2;

[0048] Fig.17 Schematic diagram of the structure of the reagent rack assembly K;

[0049] Fig.18 is a schematic structural diagram of a reagent tray 14;

[0050] Fig.19 It is a schematic diagram of the structure of the incubation detection system D;

[0051] Fig. 20 is a cross-sectional view of the incubation detection system D in the detection state;

[0052] Fig.21 It is a schematic diagram of the structure of the incubation tray 19;

[0053] Fig. 22 It is a structural schematic diagram of the rotary lifting filling system H. DETAILED DESCRIPTION

[0054] The utility model is further described below in conjunction with embodiments and drawings.

[0055] like Figure 1 and 2 As shown, a miniature low-throughput fully automatic chemiluminescence immunoassay analyzer includes a sample system A, a filling position B, a washing system C and an incubation detection system D arranged in sequence in the same straight line direction a, wherein the sample system A, a filling position B, a washing system C and an incubation detection system D are arranged in sequence in the same straight line direction a. Figure 3 As shown, the washing system C has a rotatable washing tray 1, on which a cup receiving groove 1a is integrated. A cup arranging system E is arranged on one side of the washing system C. The cup arranging channel E1 of the cup arranging system E is connected to the cup receiving groove 1a, so that the reaction cups in the cup arranging channel E1 can be discharged one by one into the cup receiving groove 1a. The incubation and detection system D integrates the incubation and detection functions. Figure 1 and Figure 2 As shown, a gripper system F is arranged above the linear direction a, and the gripper system F is used to grab and transfer the reaction cup. The gripper system F includes a gripper and a moving module for controlling the movement of the gripper, and the moving module can make the gripper move linearly along the linear direction a, and move the gripper up and down along its axis, thereby ensuring that the reaction cup grabbed by the gripper can be arbitrarily transferred between the cup receiving tank 1a, the filling position B, the washing system C and the incubation detection system D.

[0056] A reagent system G is arranged on one side of the sample system A. The reagent system G is arranged on the same side as the cup arrangement system E. Figure 1 It can be seen that the space surrounded by the reagent system G, sample system A, washing system C and cup arrangement system E is arranged with a rotary lifting filling system H. Figure 7 It can be seen that during the rotation of the rotary lifting filling system H, its filling needle b can sweep across the reagent system G, the sample system A and the filling position B.

[0057] Based on the above structural design, the detection process of the analyzer is as follows: the reaction cups of the cup arrangement system E are discharged one by one into the cup receiving slot 1a, and the gripper transfers the reaction cup to the filling position B. Thereafter, the filling needle b of the rotary lifting filling system H fills the sample to be tested in the sample system A and the reagent in the reagent system G into the reaction cup at the filling position B, and then mixes them. The gripper of the gripper system F transfers the mixed reaction cup to the incubation detection system D for incubation, and then transfers the incubated reaction cup to the washing system C for filtering and washing, and finally transfers the washed reaction cup to the detection position of the incubation detection system D for luminescence detection. The miniature low-throughput fully automatic chemiluminescence immunoassay analyzer provided in this embodiment can reduce the occupied space of the transfer station and the detection station through a reasonable and compact layout, and integrates the cup receiving function in the washing system C, and integrates the incubation and detection functions in the incubation detection system D, so as to make the internal structure of the analyzer more compact, so as to meet the requirements of low-throughput and miniaturization of the analyzer, and has the advantages of good economy and low cost.

[0058] Further, such as Figure 7 and 9 As shown, the sample system A includes a base body A1, a carrier body 4 rotatably mounted on the base body A1, and a second drive assembly 5 capable of driving the carrier body 4 to rotate, wherein loading stations are distributed on the circumferential side of the carrier body 4, and a universal connection structure 6 is provided on the loading station, and the universal connection structure 6 is used to detachably install a sample rack 7 or a TIP head rack 8, wherein a dilution station is provided between two adjacent loading stations. At least one set of positioning structures 9 is integrated on the loading station, and the positioning structure 9 can position and install a liquid consumable bottle m. The liquid consumable bottle m mentioned in this embodiment can be a diluent bottle or others. The second drive assembly 5 adopts a second motor 51, and the second motor 51 is mounted on the lower side of the base body A1, and the output shaft 52 of the second motor 51 passes through the base body A1 upward and is fixedly sleeved on the bottom of the carrier body 4.

[0059] Based on this, the loading station can be compatible with the installation of sample racks 7, TIP head racks 8 and liquid consumable bottles m, and the sample racks 7, TIP head racks 8 and liquid consumable bottles m can be integrated and assembled around the carrier 4. At the same time, the sample system A is integrated with a dilution station, which can dilute the sample when necessary according to the detection requirements. The second motor 51 drives the output shaft 52 to rotate, driving the carrier 4 to rotate, and can cooperate with the analyzer to complete the sampling, pipetting and dilution of the sample. It has the advantages of high compactness and strong versatility, and is suitable for small-batch and low-throughput analyzers.

[0060] Combination Figure 1A pipette 10 is also provided above the sample system A, which is used to grab the TIP head in the sample system A to transfer the diluent or other liquids. The dilution process is as follows: the carrier 4 rotates to the dilution station and is located below the moving track of the gripper, and the gripper places the reaction cup on the dilution station. The carrier 4 then rotates to the TIP head rack 8 and is located below the pipette 10, and then the tip of the pipette 10 is inserted into the TIP head to complete the loading of the TIP head. The carrier 4 then rotates to the liquid consumable bottle m and is located below the pipette 10, and the TIP head of the pipette 10 is used to absorb the diluent in the liquid consumable bottle m. Afterwards, the carrier 4 rotates to the dilution station and is located below the pipette 10, and the pipette 10 transfers the absorbed diluent to the reaction cup at the dilution station to complete the dilution of the liquid. With this design, the user can select the TIP head filling function, that is, the TIP head rack 8 and the pipette 10, according to whether the test requires dilution. At the same time, it does not take up additional space and has the advantages of reasonable and compact layout, high functional integration and strong practicality.

[0061] For example Figure 7 and 9 As shown, the carrier 4 includes a circular column structure 4a and a support plate 4b extending radially outward from the lower part of the column structure 4a, wherein the support plate 4b is a circular support plate. The circumferential array of loading stations is distributed in the space defined by the circumferential side of the column structure 4a and the support plate 4b. Considering this, the carrier 4 with a circular structure has a technical effect of high space utilization.

[0062] Please refer to Figure 7 The universal connection structure 6 includes a protrusion 6a formed on the side of the column structure 4a, and a hanging hole 6a1 is provided at the upper end of the protrusion 6a. Figure 8 The sample system A further comprises a sample rack 7 and a TIP head rack 8. In this embodiment, the sample rack 7 and the TIP head rack 8 have the same external structure, both of which are constructed into an arc-shaped structure. Fig. 9 As shown, the inner sides of the sample rack 7 and the TIP head rack 8 are both provided with a forward-protruding hook 7a (since the outer contours of the two racks are the same, only the sample rack 7 is shown), and the hook 7a is adapted to the hook hole 6a1, and the hook 7a can be detachably inserted in the hook hole 6a1. When the hook 7a of the sample rack 7 is inserted into the hook hole 6a1, the outer contour of the sample rack 7 is adapted to the outer edge of the support plate 4b.

[0063] Re-attend Figure 7, the outer edge of the support plate 4b is circumferentially distributed with slightly outwardly protruding convex edges 4b1, and each convex edge 4b1 is provided with a limit block 4b2 extending upward. In the present embodiment, two limit blocks 4b2 are provided on one convex edge 4b1. When the sample rack 7 or the TIP head rack 8 is installed on the loading station, the limit blocks 4b2 can prevent the rack from falling radially outward along the support plate 4b, so that the rack can be more stably assembled on the carrier 4. The positioning structure 9 includes an arc-shaped groove 9a arranged on the side of the column structure 4a, and the arc-shaped groove 9a is directly opposite to the convex edge 4b1. The arc-shaped groove 9a and the convex edge 4b1 just form a circular groove, so that the liquid consumables bottle m can be placed smoothly (refer to Fig.10 ), and the two stoppers 4b2 on the convex edge 4b1 can effectively prevent the liquid consumable bottle m from falling off. In this embodiment, two arc grooves 9a are provided on one loading station, and the two arc grooves 9a are respectively located on both sides of the convex block 6a of the loading station. Such a design increases the loading position of the liquid consumable bottle m, making the sample system A more compact.

[0064] like Figure 7 As shown, the dilution station includes a support frame 11 arranged on the upper part of the column structure 4a and extending outward, and the support frame 11 is provided with a second cup placement hole 11a for placing the reaction cup. Such a design can meet the needs of sample addition and dilution, making the system more functional. Further, two groups of second cup placement holes 11a are symmetrically arranged on the support frame 11, and a recovery channel 11b is arranged between the two groups of second cup placement holes 11a. The recovery channel 11b runs through the carrier 4 in the height direction, and the recovery channel 11b is used to recycle the TIP head after use. Such a design adds a TIP discard channel, further improving the compactness of the structure and the multifunctionality of the sample system A.

[0065] Please refer to Figure 1 and Fig.10 The miniature low-throughput fully automatic chemiluminescence immunoassay analyzer also includes a mixing mechanism J arranged in the straight line direction a, and the mixing mechanism J is arranged between the washing system C and the incubation detection system D. The mixing mechanism J includes a substrate J1, on which a third driving component 13 and a rotatable rotating seat 12 are provided, and the third driving component 13 can drive the rotating seat 12 to rotate. A cup holder 12a is rotatably mounted on the rotating seat 12, and a cup placement groove 12a1 for placing a reaction cup n is provided on the upper part of the cup holder 12a. A guide column J11 is fixedly provided on the substrate J1, and a constraint groove 12b is provided on one side of the lower part of the cup holder 12a, and the constraint groove 12b is movably sleeved on the guide column J11. Combined with Fig.10It can be seen that the rotation center line of the cup holder 12a is eccentrically arranged relative to the rotation center line of the rotating seat 12. With such a design, during the movement of the cup holder 12a driven by the third driving assembly 13, under the constraint of the constraint groove 12b and the guide column J11, the cup holder 12a has both a revolution around the rotation center line of the rotating seat 12 and a small oscillation along its rotation axis. Such a movement mode can make the liquid in the reaction cup on the cup holder 12a present a vortex state, so that the reaction cup on the cup placement groove 12a1 mixes the sample and reagent inside it in a vortex manner, which can significantly improve the mixing effect. The structural design of mixing a single reaction cup can better control the mixing state of the liquid in the reaction cup, thereby ensuring that the mixing degree and effect of the liquid in each reaction cup are consistent, which helps to ensure the stability of the subsequent test results. At the same time, the mixing mechanism is simple and compact in structure, low in cost, and also saves workstations, and is particularly suitable for low-throughput analyzers in small batches.

[0066] Please refer to Fig.10 and 11 The lower side of the cup holder 12a is provided with a holding portion 12a2 extending outward, and the restraining groove 12b is provided at the outer end of the holding portion 12a2. In this embodiment, the guide post J11 is a pin installed on the substrate J1. With such a design, after the lower part of the cup holder 12a is restricted by the guide post J11, the third motor 13a can work to drive the lower part of the cup holder 12a to eccentrically shake, thereby achieving the purpose of mixing.

[0067] In this embodiment, the upper end of the guide column J11 is in a state of active connection with the restraining groove 12b, so as to prevent the lower part of the cup holder 12a from being completely fixed, prevent the mechanism from being stuck, and thus ensure the reliability of mixing. In addition, arranging the guide column J11 and the holding portion 12a2 at the lower end of the cup holder 12a can further increase the shaking of the upper part of the cup holder 12a, so that the liquid in the reaction cup n is mixed more fully.

[0068] like Figure 3 and 5 As shown, the washing system C includes a first base C1, which has a chamber C11 with an open top, and the chamber C11 is a circular chamber. A washing tray 1 is rotatably mounted in the chamber C11, and the washing tray 1 is constructed in a circular structure. Figure 6As shown, the washing tray 1 is provided with first cup placement holes 1b in a circular array, and the first cup placement holes 1b are used to place reaction cups n, and each first cup placement hole 1b is arranged in the vertical direction. The washing tray 1 is provided with a magnetic component 3 at a position close to the bottom of each first cup placement hole 1b. The first driving component 2 is installed at the bottom of the first base C1, and the first driving component 2 can drive the washing tray 1 to rotate. During the rotation of the washing tray 1 relative to the first base C1, the magnetic component 3 can rotate synchronously with the washing tray 1. After the gripper places the reaction cup n vertically on the first cup placement hole 1b, the washing tray 1 is driven to rotate by the first driving component 2, and the first cup placement hole 1b rotates with the washing tray 1 grid by grid, so that operations such as filling of cleaning liquid, extraction of waste liquid, and filling of substrate can be realized synchronously for different reaction cups. During the rotation of the reaction cup n with the washing tray 1, since the reaction cup n and the magnetic component 3 remain relatively fixed, that is, the magnetic component 3 can rotate synchronously with the reaction cup n, it is ensured that the bottom of the reaction cup n can be continuously in a stable magnetic field environment during the entire washing process, which can not only enhance the magnetic separation effect of the magnetic particles in the cup, but also improve the consistency of the magnetic separation effect, thereby improving the detection accuracy of the final analyzer.

[0069] Further, such as Figure 6 As shown, the number of the first cup placement hole 1b and the number of magnetic components 3 are four groups, and the four groups of magnetic components 3 are distributed in a square at the bottom of the washing tray 1, and each magnetic component 3 is installed on the inner side of the lower part of the corresponding first cup placement hole 1b. Adopting such a layout method can enhance the magnetic flux of the magnetic field and improve the magnetic analysis effect. Further, each magnetic component 3 includes three magnetic blocks. In the same group of magnetic components 3, the three magnetic blocks are a vertical magnetic block 3a and a horizontal magnetic block 3b close to both ends of the vertical magnetic block 3a, wherein the vertical magnetic block 3a is directly opposite to the side of the first cup placement hole 1b, and the vertical magnetic block 3a is flush with the horizontal magnetic block 3b on one side close to the first cup placement hole 1b, and the other end protrudes from the horizontal magnetic block 3b. Such a design has the technical advantages of reasonable and compact structural layout, further enhances the magnetic flux of the magnetic field, and improves the magnetic separation effect.

[0070] Please refer to Figure 3 and Figure 6 The washing tray 1 has a flange 1c extending radially outward on the upper part, and cup placement notches are evenly distributed on the flange 1c, and the cup placement notches constitute a cup receiving groove 1a. Figure 2 and Figure 4 It can be seen that the opening of the cup receiving groove 1a can be directly connected to the cup arranging channel E1 of the cup arranging system E in the analyzer, so that the reaction cup in the cup arranging channel E1 can be directly slid into the cup receiving groove 1a, which significantly improves the detection efficiency of the analyzer and helps to achieve a compact design of the product.

[0071] Please refer to Figure 4 and5 , a liquid supply and extraction system C2 is provided above the first base C1, and the liquid supply and extraction system C2 can add liquid to the reaction cup n or extract waste liquid. The liquid supply and extraction system C2 includes a washing needle C21 located above the first base C1, and the washing needle C21 is equipped with a liquid line tube C22. In the process of washing the liquid sample, the temperature of the washing liquid, the substrate liquid and the sample liquid should be kept appropriate. Therefore, the chamber C11 is surrounded by a heating belt and an insulation layer. The heating belt heats up the inside of the chamber C11 to ensure the ambient temperature of the reaction cup n, and the insulation layer can keep the internal temperature of the chamber C11 constant after heating. At the same time, when in use, the liquid line tube C22 is set inside the chamber C11, and the liquid in the liquid line tube C22 can be heated to the required temperature in advance to ensure that the temperature of the liquid in the reaction cup n is controlled within a more precise range, which helps to improve the final detection accuracy of the chemiluminescence immunoassay.

[0072] like Figures 12 to 14 As shown, the reagent system G includes a reagent bin G1, a refrigeration module G2 and a fourth drive assembly G3, wherein a reagent tray 14 for storing reagents is rotatably installed in the reagent bin G1, the fourth drive assembly G3 is arranged at the bottom of the reagent bin G1, and can drive the reagent tray 14 to rotate, and a first assembly notch d is arranged on the side of the reagent bin G1. As shown in FIG15, the refrigeration module G2 includes a semiconductor refrigeration element 15 and a cold circulation fan 16, wherein the semiconductor refrigeration element 15 has a hot side 15a and a cold side 15b, the semiconductor refrigeration element 15 is installed at the first assembly notch d, and the cold side 15b is located inside the reagent bin G1, and the hot side 15a is located outside the reagent bin G1, and the cold circulation fan 16 is installed on the cold side 15b, and the air outlet direction of the cold circulation fan 16 is directly facing the side of the reagent bottle. With such a design, the semiconductor refrigeration element 15 of the refrigeration module G2 is directly mounted on the first assembly notch d on the side of the reagent bin G1, and the cold side 15b of the semiconductor refrigeration element 15 is located inside the reagent bin G1, so that the cold side 15b can be directly opposite to the side of the reagent bottle on the reagent tray 14, so that the cold capacity of the semiconductor refrigeration element 15 can be directly applied to the reagent bottle. With the rotation of the reagent tray 14, the reagents in each reagent bottle can be fully cooled down, meeting the insulation and refrigeration requirements of the reagents in the reagent bin G1. At the same time, through the cold circulation fan 16, cold air can be blown into the inside of the reagent bin G1, accelerating the air circulation speed and heat exchange speed inside the reagent bin G1, so that the temperature inside the reagent bin G1 can be quickly reduced, and the cooling efficiency is improved. Since the hot side 15a of the semiconductor refrigeration element 15 is located outside the reagent bin G1, the hot air on the hot side 15a of the semiconductor refrigeration element 15 can be discharged to the outside of the reagent bin G1 in time to achieve rapid heat dissipation. Such a structural design is not only simple and compact, but also does not occupy extra height space, further ensuring the compactness and miniaturization of the system, and is particularly suitable for small-batch, low-throughput chemiluminescence immunoassay analyzers.

[0073] For further information, see Fig.12 and 13 The refrigeration module G2 also includes a module shell G21 installed on the outside of the first assembly notch d. The module shell G21 has a through heat dissipation channel f inside. The hot side 15a of the semiconductor refrigeration element 15 is located in the heat dissipation channel f. A heat dissipation fan G4 is provided at one end of the heat dissipation channel f. Under the action of the heat dissipation fan G4, the hot air on the hot side 15a can be quickly discharged from the other end of the heat dissipation channel f, further accelerating the heat dissipation efficiency.

[0074] Re-attend Fig.15 and 16 The cold side 15b of the semiconductor refrigeration element 15 is fixed with an array of cold end fins 15c, and a cold circulation fan 16 is arranged at the outer end of the cold end fin 15c. The air is contacted with the cold end fin 15c for heat exchange, so that the air temperature is reduced and the air is sent into the reagent chamber G1 by the cold circulation fan 16.

[0075] Please refer to Fig.15 The hot side 15a of the semiconductor cooling element 15 is equipped with hot end fins 15a1, which are arranged in an array in the vertical direction, and are located in the heat dissipation channel f. The air in the heat dissipation channel f can pass through the gaps between the hot end fins 15a1. With this design, under the action of the heat dissipation fan G4, the air in the heat dissipation channel f can fully contact the hot end fins 15a1, thereby helping the hot side 15a of the semiconductor cooling element 15 to quickly dissipate heat.

[0076] like Fig.13 As shown, in this embodiment, the outer shell of the reagent chamber G1 is constructed to be close to a cubic structure, and both sides of the first assembly notch d thereof are provided with arc segments G12 extending outward. The semiconductor refrigeration element 15 is arranged on the side of the module shell G21 away from the end of the heat dissipation fan G4, and the semiconductor refrigeration element 15 on the side of the module shell G21 is directly fixed and embedded between the two arc segments G12 to ensure the installation space of the cold side 15b, the cold end fin 15c and the cold circulation fan 16, and the other side of the module shell G21 has an inclined segment G211 and a parallel segment G212 connected in sequence, wherein the inclined segment G211 gradually inclines inward along the end where the heat dissipation fan G4 is installed, so that the heat dissipation channel f gradually narrows to the position of the parallel segment G212 along the end where the heat dissipation fan G4 is installed, and such a structural design can ensure the heat dissipation effect while miniaturizing and compacting the structure as much as possible, reducing the occupied space, and can be suitable for small-batch, low-throughput chemiluminescence immunoassay analyzers. In addition, the inclined section G211 can make the internal space at one end of the heat dissipation fan G4 significantly larger than the air outlet end, thereby ensuring that sufficient air volume flows through the hot end fins 15a1.

[0077] Please refer to Fig.17 and 18 , the reagent tray 14 is provided with a clamping structure distributed along the circumference, and a reagent rack assembly K is detachably placed on the clamping structure, and the reagent rack assembly K includes a basic reagent rack 17 and an extended reagent rack 18, wherein the basic reagent rack 17 is a strip-shaped structure, and the basic reagent rack 17 is provided with three basic installation holes 17a distributed along the linear direction, and the three basic installation holes 17a all extend along the height direction of the basic reagent rack 17, and the extended reagent rack 18 is provided with an extended installation hole 18a extending along the height direction, and the extended reagent rack 18 is detachably mounted on one side of the basic reagent rack 17. Both the basic installation hole 17a and the extended installation hole 18a are used to place reagent bottles.

[0078] Based on this, by designing the basic reagent rack 17 and the extended reagent rack 18 to be detachably connected, the basic reagent rack 17 with three mounting holes can be selected according to the detection requirements, or the basic reagent rack 17 can be expanded into a reagent rack with four mounting holes by matching the extended reagent rack 18, which meets the storage requirements of three-component or four-component reagents in the detection process, and has the technical advantages of easy installation, strong versatility and low cost. Another convenience is that in production and manufacturing, only the basic reagent rack 17 with three mounting holes and the extended reagent rack 18 with a single mounting hole need to be produced, which can reduce costs and simplify production steps. At the same time, the design of the reagent tray 14 can be compatible with the reagent rack of the three-component reagent and the reagent rack of the four-component reagent, and at the same time meets the requirements of the storage of the three-component or four-component reagents, so that the project detection of different detection requirements can be met, and it has the advantages of strong practicality and high compactness.

[0079] In this embodiment, the extended reagent rack 18 is disposed in the middle of one side in the width direction of the basic reagent rack 17, corresponding to the middle position of the three basic installation holes 17a.

[0080] Further, such as Fig.17 As shown, a hanging portion 17b is provided on one side of the width direction of the basic reagent rack 17, and a hanging buckle 18b adapted to the hanging portion 17b is provided on the side of the extended reagent rack 18, so as to facilitate the detachable installation of the extended reagent rack 18 on one side of the basic reagent rack 17. The hanging portion 17b is located between two adjacent basic installation holes 17a, which can ensure the integrity of the accommodation space of each basic installation hole 17a.

[0081] For example Fig.18As shown, the reagent tray 14 is a circular tray, and the positioning structure includes first mounting grooves 14a distributed in a circumferential array, and the first mounting grooves 14a are used to vertically place the basic reagent rack 17, so that the centers of the three basic mounting holes 17a on the same basic reagent rack 17 are located on the same radius. A second mounting groove 14b is provided between two adjacent first mounting grooves 14a, and the second mounting groove 14b is used to place the extended reagent rack 18 attached to the side of the basic reagent rack 17. With such a design, whether the test project requires a three-component reagent or a four-component reagent, the reagent tray 14 can be compatible.

[0082] Please refer to Fig.18 , the space surrounded by the inner sides of the two adjacent first mounting grooves 14a is a fan-shaped structure, and the second mounting groove 14b is preferably located in the middle of the fan-shaped structure. Designed in this way, the compactness of the structure can be improved and the space utilization rate can be increased. Another convenience is that the expansion reagent rack 18 is designed in the middle of one side of the width direction of the basic reagent rack 17, and after the expansion reagent rack 18 is installed on the basic reagent rack 17, the upper surfaces of the two reagent racks are flush. The advantage of such a design is that when the reagent structure on the reagent tray 14 is laid out, there is no need to expand the diameter of the reagent tray 14, that is, it can be compatible with the expansion reagent rack 18, and at the same time, the height of the overall structure will not be increased, which is conducive to the compact and miniaturized design of the product.

[0083] Further, refer to Fig.18 , a third mounting groove 14c is also provided in the fan-shaped space surrounded by the two adjacent first mounting grooves 14a, and the third mounting groove 14c is located outside the second mounting groove 14b, and is used to place the liquid consumable bottle m. When the liquid consumable bottle and the basic reagent rack 17 with the extended reagent rack 18 are placed on the reagent tray 14, the center lines of the reagent bottles inside each basic reagent rack 17 are located on the same circumference, the reagent bottles in the middle of each basic reagent rack 17 and the reagent bottles on each extended reagent rack 18 are located on the same circumference, and the reagent bottles outside each basic reagent rack 17 and each liquid consumable bottle are located on the same circumference. With such a layout, consumable stations are added, and more reagent bottles can be installed on the reagent tray 14 as much as possible, which improves the space utilization rate and makes the structural layout more reasonable and compact.

[0084] like Fig.19 and 20 As shown, the incubation detection system D includes a cabin D1, an incubation tray 19 is rotatably mounted inside the cabin D1, and a fifth driving assembly 20 capable of driving the incubation tray 19 to rotate is provided at the bottom of the cabin D1. Fig.21It can be seen that the incubation tray 19 is constructed as a cylindrical structure, on which are provided a plurality of circles of incubation holes 19a distributed in a circular array, and the incubation holes 19a are used to place the reaction cups n. Since the analyzer provided in this embodiment is of low-throughput and miniaturized design, two circles of incubation holes 19a are arranged on the incubation tray 19, wherein at least one of the incubation holes 19a located in the outermost circle is defined as a detection hole position 19b. A detection window 19c is provided on the side of the incubation tray 19, which is opposite to and connected to the lower part of the detection hole position 19b, and the detection window 19c exposes the lower part of the reaction cup n located in the detection hole position 19b. In combination with Fig.19 As shown, a second assembly notch e is provided on the side of the chamber D1, and a detection module 21 is fixedly installed at the position of the second assembly notch e. ​​The light-emitting detection element 21a of the detection module 21 faces the interior of the chamber D1 and is arranged at the same height as the detection window 19c. When the reaction cup n to be tested on the detection hole 19b stops at the position of the detection module 21, the light-emitting detection element 21a can face the detection window 19c of the detection hole 19b, and the light-emitting detection element 21a can work to complete the analysis and detection.

[0085] Based on this, the outermost incubation holes 19a are directly used as the detection holes 19b, and the detection window 19c is set at the position corresponding to the detection hole 19b on the side of the incubation plate 19, without the need for additional layout and setting of detection stations, the two functions of incubation and detection can be more reasonably integrated into one module, which has the advantages of simple structure, high integration and compact product. At the same time, it can also optimize the moving stroke of the gripper in the analyzer and improve the detection efficiency.

[0086] In this embodiment, the fifth driving component 20 drives the incubation plate 19 to rotate by direct motor drive, which has the technical advantages of high rotation accuracy and strong stability, and is particularly suitable for equipment with high position accuracy requirements in such chemiluminescent immunoassay analyzers.

[0087] like Fig. 22As shown, the rotary lifting filling system H comprises a second base H1, on which a column 22 is provided which can rotate and slide up and down relative to the second base H1, and a lifting driving assembly 24 which drives the column 22 to slide up and down, and a rotating driving assembly 25 which drives the column 22 to rotate are arranged. A cantilever 23 is provided on the top of the column 22, and a filling needle b is arranged at the far end of the cantilever 23. By controlling the rotation and up and down movement of the column 22, the filling needle b can be driven to rotate horizontally and to rise and fall. Further, the second base H1 comprises a first vertical plate 26 and a second vertical plate 27 arranged in the vertical direction, and a supporting bottom plate 28 is welded to the lower end of the first vertical plate 26 and the second vertical plate 27, and a mounting top plate 29 is welded to the upper end. The first vertical plate 26, the second vertical plate 27, the supporting bottom plate 28 and the mounting top plate 29 are constructed into a cubic structure, and the interior of the cubic structure encloses an assembly space H2, and the lower part of the column 22 can be slid up and down in the assembly space H2. The lifting drive assembly 24 is arranged on the second vertical plate 27 , and the rotating drive assembly 25 is arranged on the mounting top plate 29 .

[0088] With this design, the second base H1 is welded by four plates to form a cubic structure, and sufficient installation space is reserved inside, which can significantly reduce the difficulty of integrated assembly of the column 22, the lifting drive assembly 24 and the rotating drive assembly 25, thereby helping to improve the movement accuracy of each mechanism after the rotary lifting filling system H is assembled, and ensure the accuracy of the moving position of the filling needle b. In addition, the second base H1 composed of four sheet metal parts also has the advantages of high support strength, low production cost, light weight, thin thickness, and simple process, which can greatly reduce the overall weight of the equipment and reduce the assembly area and cost.

[0089] Finally, it should be noted that the above description is only a preferred embodiment of the present invention. Under the guidance of the present invention, ordinary technicians in this field can make a variety of similar expressions without violating the purpose and claims of the present invention, and such changes fall within the scope of protection of the present invention.

Claims

1. A miniature low-throughput fully automatic chemiluminescence immunoassay analyzer, characterized in that: The invention comprises a sample system (A), a filling position (B), a washing system (C) and an incubation detection system (D) arranged in sequence in the same straight line direction (a), wherein the washing system (C) has a rotatable washing tray (1), the washing tray (1) is integrated with a cup receiving groove (1a), a cup arranging system (E) is arranged on one side of the washing system (C), and the cup arranging system (E) can discharge reaction cups one by one into the cup receiving groove (1a); the incubation detection system (D) is integrated with incubation and detection functions; A gripper system (F) is arranged above the straight line direction (a), and the gripper system (F) can transfer the reaction cup arbitrarily between the cup receiving tank (1a), the filling position (B), the washing system (C) and the incubation detection system (D); A reagent system (G) is arranged on one side of the sample system (A), and a rotary lifting filling system (H) is arranged between the reagent system (G) and the sample system (A) on the side close to the washing system (C). During the rotation of the rotary lifting filling system (H), its filling needle (b) can sweep across the reagent system (G), the sample system (A) and the filling position (B).

2. The miniature low-throughput fully automatic chemiluminescence immunoassay analyzer according to claim 1, characterized in that: The sample system (A) comprises a base (A1), a carrier (4) rotatably mounted on the base (A1), and a second driving assembly (5) for driving the carrier (4) to rotate, wherein loading stations are distributed on the circumferential side of the carrier (4), and a universal connecting structure (6) is provided on the loading station, and the universal connecting structure (6) is used for detachably mounting a sample rack (7) or a TIP head rack (8), wherein a dilution station is provided between two adjacent loading stations; At least one set of positioning structures (9) is integrated on the loading station, and the positioning structures (9) are used to position and install the liquid consumable bottle (m).

3. The miniature low-throughput fully automatic chemiluminescence immunoassay analyzer according to claim 2, characterized in that: It also includes a pipette (10), which is arranged above the sample system (A) and is used to grab the TIP head in the sample system (A) to transfer liquid.

4. The miniature low-throughput fully automatic chemiluminescence immunoassay analyzer according to claim 2, characterized in that: The carrier (4) comprises a column structure (4a) and a support plate (4b) extending radially outward from the lower part of the column structure (4a); the loading station is arranged in a circular array on the circumferential side of the column structure (4a); the dilution station comprises a support frame (11) arranged on the upper part of the column structure (4a) and extending outward; the support frame (11) is provided with a second cup placement hole (11a) for placing a reaction cup.

5. The miniature low-throughput fully automatic chemiluminescence immunoassay analyzer according to claim 1, characterized in that: The invention also comprises a mixing mechanism (J) arranged in the linear direction (a), the mixing mechanism (J) comprising a base plate (J1), a rotating seat (12) rotatably mounted on the base plate (J1), and a third driving assembly (13) for driving the rotating seat (12) to rotate, the rotating seat (12) being rotatably mounted with a cup holder (12a), the upper part of the cup holder (12a) being provided with a cup placement groove (12a1) for placing a reaction cup, the base plate (J1) being fixedly provided with a guide column (J11), a side of the lower part of the cup holder (12a) being provided with a restraining groove (12b), the restraining groove (12b) being movably sleeved on the guide column (J11); The rotation centerline of the cup seat (12a) is eccentrically arranged relative to the rotation centerline of the rotating seat (12); under the restraining action of the restraining groove (12b) and the guide column (J11), when the third driving component (13) drives the rotating seat (12) to rotate, the cup seat (12a) has both orbital revolution and slight oscillation along its rotation axis.

6. The miniature low-throughput fully automatic chemiluminescence immunoassay analyzer according to claim 1, characterized in that: The washing system (C) comprises a first base (C1) and a first driving assembly (2); the washing tray (1) is rotatably mounted in the first base (C1); the first driving assembly (2) is used to drive the washing tray (1) to rotate; first cup placement holes (1b) are distributed in a circular array on the washing tray (1); each of the first cup placement holes (1b) is arranged in a vertical direction; a magnetic assembly (3) is installed on the washing tray (1) at a position close to the bottom of each of the first cup placement holes (1b); and when the washing tray (1) rotates relative to the first base (C1), the magnetic assembly (3) can rotate synchronously with the washing tray (1); The upper part of the washing tray (1) is provided with a flange (1c) extending outwards, and cup placement notches are evenly distributed on the flange (1c), and the cup placement notches constitute the cup receiving groove (1a).

7. The miniature low-throughput fully automatic chemiluminescence immunoassay analyzer according to claim 1, characterized in that: The reagent system (G) comprises a reagent bin (G1), a refrigeration module (G2) and a fourth drive assembly (G3); a reagent tray (14) for storing reagents is rotatably mounted in the reagent bin (G1); and the fourth drive assembly (G3) is used to drive the reagent tray (14) to rotate; The reagent chamber (G1) is provided with a first assembly notch (d) on its side, and the refrigeration module (G2) comprises a semiconductor refrigeration element (15) and a cold circulation fan (16), wherein the semiconductor refrigeration element (15) has a hot side (15a) and a cold side (15b), the semiconductor refrigeration element (15) is installed at the first assembly notch (d), and the cold side (15b) is located inside the reagent chamber (G1), and the hot side (15a) is located outside the reagent chamber (G1), and the cold circulation fan (16) is installed on the cold side (15b).

8. The miniature low-throughput fully automatic chemiluminescence immunoassay analyzer according to claim 7, characterized in that: The reagent tray (14) is provided with a locking structure distributed along the circumference, and a reagent rack assembly (K) is detachably placed on the locking structure; The reagent rack assembly (K) comprises a basic reagent rack (17), the basic reagent rack (17) being provided with three basic mounting holes (17a) distributed in a linear direction, each of the basic mounting holes (17a) extending in a height direction of the basic reagent rack (17), an extended reagent rack (18) being detachably mounted on one side of the basic reagent rack (17), the extended reagent rack (18) being provided with an extended mounting hole (18a) extending in a height direction, the extended mounting hole (18a) and the three basic mounting holes (17a) being used for placing reagent bottles.

9. The miniature low-throughput fully automatic chemiluminescence immunoassay analyzer according to claim 1, characterized in that: The incubation detection system (D) comprises a cabin (D1) and an incubation tray (19) rotatably arranged inside the cabin (D1); a fifth driving assembly (20) for driving the incubation tray (19) to rotate is provided at the bottom of the cabin (D1); the incubation tray (19) is constructed as a cylindrical structure, and is provided with a plurality of circles of incubation holes (19a) distributed in a circular array, wherein at least one of the incubation holes (19a) located in the outermost circle is defined as a detection hole position (19b); a detection window (19c) is provided on the side of the incubation tray (19) and is directly opposite to and connected to the lower part of the detection hole position (19b); the detection window (19c) exposes the lower part of the reaction cup located in the detection hole position (19b); A second assembly notch (e) is provided on the side of the cabin (D1), and a detection module (21) is fixedly installed at the position of the second assembly notch (e). The light-emitting detection element (21a) of the detection module (21) faces the interior of the cabin (D1) and is arranged at the same height as the detection window (19c).

10. The miniature low-throughput fully automatic chemiluminescence immunoassay analyzer according to claim 1, characterized in that: The rotary lifting filling system (H) comprises a second base (H1), the second base (H1) is provided with a column (22) which can rotate relative to the second base (H1) and slide up and down, the top of the column (22) is provided with a cantilever (23), and the filling needle (b) is arranged at the far end of the cantilever (23); The second base (H1) is provided with a lifting drive assembly (24) for driving the column (22) to slide up and down, and a rotating drive assembly (25) for driving the column (22) to rotate. The second base (H1) comprises a first vertical plate (26) and a second vertical plate (27) arranged in a vertical direction. A supporting bottom plate (28) is welded to the lower ends of the first vertical plate (26) and the second vertical plate (27), and a mounting top plate (29) is welded to the upper ends. The first vertical plate (26), the second vertical plate (27), the supporting bottom plate (28) and the mounting top plate (29) are constructed into a cubic structure, and an assembly space (H2) is enclosed therein. The lower part of the column (22) is arranged in the assembly space (H2) so as to slide up and down, the lifting drive assembly (24) is arranged on the second vertical plate (27), and the rotating drive assembly (25) is arranged on the mounting top plate (29).

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