Constant-temperature fluorescent PCR (polymerase chain reaction) nucleic acid detection kit for food-borne escherichia coli O157

Abstract

The utility model discloses a constant-temperature fluorescent PCR (polymerase chain reaction) nucleic acid detection kit for food-borne escherichia coli O157, and relates to the technical field of detection kits. The plurality of cutting parts are fixed at the lower end in the box body at equal intervals and are used for cutting the reagent tubes into a plurality of groups; the plurality of clamping devices are arranged above the cutting part in a sliding manner, and are used for clamping the reagent tubes; the lifting assembly is mounted in the box body in a manner of sliding up and down, and is positioned above the clamping device; the lifting assembly and the clamping device are arranged in a matched mode to clamp and fix a reagent tube when the cover plate assembly is buckled, meanwhile, the lifting assembly slides to the upper portion, the upper portion of the reagent tube is limited, the reagent tube is prevented from shaking in the transportation process, clamping and fixing of the reagent tube are relieved when the cover plate assembly is taken down, and therefore the reagent tube is prevented from being damaged. The reagent tube is convenient to take and place.

Description

Technical Field

[0001] The utility model relates to the technical field of detection kits, in particular to a foodborne Escherichia coli O157 constant temperature fluorescent PCR nucleic acid detection kit. Background Art

[0002] The public health problem caused by foodborne pathogenic microorganisms is a major problem in the world, and its missed detection rate is as high as over 95%. According to WHO estimates, no less than 2 million people die each year from foodborne microbial infectious diarrhea, most of which are due to microbial contamination of food and drinking water. Enterohemorrhagic Escherichia coli (EHEC) occupies an important hygienic position in foodborne pathogenic microorganism contamination.

[0003] Nuclease mimetics are peroxidase-like complexes of nucleic acids, which are usually single-stranded DNA / RNA. Nuclease mimetics can fold into stable and catalytically active G-quadruplexes. In the presence of hemin, they bind to hemin and catalyze some chemical reactions to produce color changes, which can be used for naked eye detection. Combining nuclease mimetics with traditional PCR can not only avoid the contamination of nucleic acid dyes, reduce the requirements for experimental equipment, simplify the operating steps of traditional PCR detection, and improve the sensitivity of the detection method, but more importantly, make the detection visible to the naked eye and can also be combined with other instruments for quantitative detection.

[0004] The existing reagent tubes are usually fixed by a clamping device during carrying, and the use of the clamping device will have a certain degree of influence on the taking and placing of the reagent tubes. Although not relying on the clamping device makes it easier to take and place the reagent tubes, the reagent tubes are more likely to shake during carrying.

[0005] Therefore, it is necessary to invent a foodborne Escherichia coli O157 constant temperature fluorescent PCR nucleic acid detection kit to solve the above problems. Utility Model Content

[0006] The purpose of the utility model is to provide a foodborne Escherichia coli O157 constant temperature fluorescent PCR nucleic acid detection kit to solve the problem proposed in the above background technology that the reagent tube is usually fixed by a clamping device during carrying, and the use of the clamping device will cause a certain degree of influence on the taking and placing of the reagent tube. Although not relying on the clamping device facilitates the taking and placing of the reagent tube, the reagent tube is more likely to shake during carrying.

[0007] To achieve the above purpose, the utility model provides the following technical solution: a foodborne Escherichia coli O157 constant temperature fluorescent PCR nucleic acid detection kit, comprising:

[0008] Box;

[0009] A dividing piece, wherein a plurality of the dividing pieces are provided and fixed at an equal distance at the lower end of the inner part of the box body, and divide the reagent tubes into a plurality of groups;

[0010] A clamping device, wherein a plurality of the clamping devices are provided and slidably mounted above the dividing piece to clamp the reagent tube;

[0011] A lifting assembly, which is slidably mounted inside the box body and located above the clamping device, and pulls the clamping device to clamp the reagent tube when the lifting assembly rises;

[0012] A cover plate assembly, wherein the cover plate assembly is detachably fixed at the top opening of the box body;

[0013] The magnetic component is installed on the lifting component and the cover component to form a structure in which the cover component pulls the lifting component to rise after installation.

[0014] Optionally, the dividing piece includes:

[0015] A partition plate, the two ends of which are fixedly connected to the inner walls of the box at both ends;

[0016] Two first rubber pads are provided and bonded to two sides of the partition plate respectively;

[0017] A sliding through hole, which is provided on the partition plate and passes through the partition plate vertically;

[0018] The dividing blocks are provided in plurality and fixed in the sliding through hole at equal distances.

[0019] Optionally, the clamping device comprises:

[0020] A clamping plate, which is slidably mounted inside the box and contacts the upper surface of the partition plate;

[0021] A second rubber pad is bonded to a side of the clamping plate close to the reagent tube.

[0022] Optionally, the clamping device further comprises:

[0023] A sliding block, which is fixed to the bottom of the clamping plate and can be slidably inserted into the interior of the sliding through hole;

[0024] A compression spring, the two ends of which are fixedly connected to the sliding block and the dividing block respectively;

[0025] An elastic drawstring is fixed on the sliding block and passes through the dividing block to be fixedly connected with the lifting assembly.

[0026] Optionally, the lifting assembly includes:

[0027] A lifting plate, which is installed inside the box body so as to be able to slide up and down;

[0028] A plurality of positioning through holes are provided, and the positioning through holes are opened on the lifting plate in a rectangular array and penetrate vertically;

[0029] A rubber ring is bonded to the inside of the positioning through hole.

[0030] Optionally, the cover plate assembly includes:

[0031] A cover plate, the cover plate being detachably fixed on the top of the box body;

[0032] A sealing gasket is clamped at the connection between the cover plate and the box body.

[0033] Optionally, the magnetic component includes:

[0034] Four first magnets are provided and fixed at the four corners of the bottom of the cover plate;

[0035] The second magnets are provided in four numbers and fixed at the four corners of the top of the lifting plate and attracted to the first magnets.

[0036] Technical effects and advantages of the utility model:

[0037] 1. The utility model clamps and fixes the reagent tube when the cover assembly is buckled on by cooperating with the lifting assembly and the clamping device. At the same time, the lifting assembly slides upward to limit the upper part of the reagent tube to prevent the reagent tube from shaking during transportation. When the cover assembly is removed, the reagent tube is released from being clamped and fixed, which facilitates the taking and placing of the reagent tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 This is a schematic diagram of the internal structure of the utility model;

[0039] Figure 2 This is a schematic diagram of the main structure of the utility model;

[0040] Figure 3 This is a schematic diagram of the structure of the lifting component of the utility model;

[0041] Figure 4 This is a schematic diagram of the structure of the clamping device of the utility model;

[0042] Figure 5 This is a schematic diagram of the cover structure of the utility model.

[0043] In the figure: 100, box body;

[0044] 200, dividing piece; 210, dividing plate; 220, first rubber pad; 230, sliding through hole; 240, dividing block;

[0045] 300, clamping device; 310, clamping plate; 320, second rubber pad; 330, sliding block; 340, compression spring; 350, elastic pull rope;

[0046] 400, lifting assembly; 410, lifting plate; 420, positioning through hole; 430, rubber ring;

[0047] 500, cover plate assembly; 510, cover plate; 520, sealing gasket;

[0048] 600, magnetic assembly; 610, first magnet; 620, second magnet. DETAILED DESCRIPTION

[0049] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0050] The utility model provides Figure 1-5 A foodborne Escherichia coli O157 constant temperature fluorescent PCR nucleic acid detection kit is shown, comprising:

[0051] Box 100;

[0052] A plurality of dividing pieces 200 are provided and fixed at the lower end of the box body 100 at equal distances to divide the reagent tubes into multiple groups;

[0053] A clamping device 300, wherein a plurality of clamping devices 300 are provided and are slidably mounted above the dividing member 200 to clamp the reagent tube;

[0054] The lifting assembly 400 is installed inside the box 100 so as to slide up and down, and is located above the clamping device 300. When the lifting assembly 400 rises, the clamping device 300 is pulled to clamp the reagent tube;

[0055] A cover plate assembly 500, the cover plate assembly 500 is detachably fixed at the top opening of the box body 100;

[0056] The magnetic assembly 600 is installed on the lifting assembly 400 and the cover assembly 500, forming a structure in which the cover assembly 500 pulls the lifting assembly 400 to rise after installation.

[0057] Among them, the lifting component 400 and the clamping device 300 are arranged in cooperation to clamp and fix the reagent tube when the cover component 500 is buckled on it. At the same time, the lifting component 400 slides upward to limit the upper part of the reagent tube to prevent the reagent tube from shaking during transportation. When the cover component 500 is removed, the reagent tube is released from being clamped and fixed, which facilitates the taking and placing of the reagent tube.

[0058] In some embodiments of the present invention, the partition 200 includes:

[0059] A partition plate 210, two ends of which are fixedly connected to the inner walls of the box body 100;

[0060] Two first rubber pads 220 are provided and bonded to two sides of the partition plate 210 respectively;

[0061] A sliding through hole 230, which is formed on the partition plate 210 and passes through the partition plate 210 vertically;

[0062] The dividing block 240 is provided in plurality and fixed at equal distances inside the sliding through hole 230 .

[0063] The dividing plate 210 is used to divide the multiple reagent tubes into multiple groups, and prevents the multiple groups of reagent tubes from shaking and colliding during transportation;

[0064] The first rubber pad 220 is provided to prevent the dividing plate 210 from scratching or damaging the reagent tube;

[0065] Among them, the setting of the sliding through hole 230 provides space for the installation and sliding of the clamping device 300, so that the clamping device 300 can slide normally. At the same time, the setting of the dividing block 240 ensures the normal installation and fixation of the clamping device 300, and divides multiple clamping devices 300.

[0066] In some embodiments of the present invention, the clamping device 300 includes:

[0067] A clamping plate 310, the clamping plate 310 is slidably mounted inside the box body 100 and contacts the upper surface of the partition plate 210;

[0068] The second rubber pad 320 is bonded to the side of the clamping plate 310 close to the reagent tube.

[0069] The clamping plate 310 is provided to clamp and fix the reagent tube by sliding, or to release the clamping of the reagent tube;

[0070] The provision of the second rubber pad 320 can prevent the clamping plate 310 from scratching or damaging the reagent tube.

[0071] In some embodiments of the present invention, the clamping device 300 further includes:

[0072] A sliding block 330, which is fixed to the bottom of the clamping plate 310 and can be slidably inserted into the interior of the sliding through hole 230;

[0073] A compression spring 340, two ends of which are fixedly connected to the sliding block 330 and the dividing block 240 respectively;

[0074] The elastic pull rope 350 is fixed on the sliding block 330 and passes through the dividing block 240 to be fixedly connected with the lifting assembly 400 .

[0075] The sliding block 330 and the sliding through hole 230 are matched to guide the sliding of the clamping device 300, thereby preventing the clamping device 300 from deviating during the sliding clamping.

[0076] Among them, the arrangement of the compression spring 340 can release the elastic force portion of the compression spring 340 after the cover assembly 500 is removed, driving the lifting assembly 400 to descend and push the clamping plate 310 away from the reagent tube, thereby releasing the clamping of the reagent tube;

[0077] The elastic pull rope 350 is set to pull the clamping plate 310 to move when the lifting assembly 400 rises, clamp the reagent tube, and drive the lifting assembly 400 to descend when the pressure of the compression spring 340 is released.

[0078] In some embodiments of the present invention, the lifting assembly 400 includes:

[0079] A lifting plate 410, the lifting plate 410 is installed inside the box body 100 so as to be slidable up and down;

[0080] Positioning through holes 420, a plurality of positioning through holes 420 are provided and are opened on the lifting plate 410 in a rectangular array and penetrate up and down;

[0081] The rubber ring 430 is bonded inside the positioning through hole 420 .

[0082] The upper part of the reagent tube is limited by the rise of the lifting plate 410 to prevent the reagent tube from shaking during the transportation process;

[0083] The positioning through hole 420 and the rubber ring 430 provide space for the insertion of the reagent tube, so that the reagent tube can be divided and placed.

[0084] In some embodiments of the present invention, the cover plate assembly 500 includes:

[0085] A cover plate 510, the cover plate 510 is detachably fixed on the top of the box body 100;

[0086] The sealing gasket 520 is clamped at the connection between the cover plate 510 and the box body 100 .

[0087] The cover plate 510 is provided to ensure that the box 100 can be opened normally, so that the reagent tube can be normally placed inside the box 100;

[0088] The sealing gasket 520 is provided to ensure the sealing of the connection between the cover plate 510 and the box body 100;

[0089] A handle 530 is fixedly mounted on the top of the cover plate 510 to facilitate carrying of the detection kit, so that the detection kit can be carried to various places.

[0090] In some embodiments of the present invention, the magnetic assembly 600 includes:

[0091] First magnets 610, four of which are provided and fixed at the four bottom corners of the cover plate 510;

[0092] The second magnets 620 are provided in four numbers and fixed at the four corners of the top of the lifting plate 410 and attracted to the first magnets 610 .

[0093] The cooperation of the first magnet 610 and the second magnet 620 can adsorb the lifting plate 410 to rise after the cover plate 510 is installed, and the magnetic force of the first magnet 610 and the second magnet 620 is greater than the elastic force of the compression spring 340 .

[0094] Working method of the utility model:

[0095] When placing the reagent tube, the reagent tube is passed through the rubber ring 430 and inserted between the two partition plates 210, and then the cover plate 510 is fixed at the top opening of the box body 100, and then the first magnet 610 and the second magnet 620 attract each other and pull the lifting plate 410 up, so that the lifting plate 410 pulls the sliding block 330 and the clamping plate 310 to slide through the elastic pull rope 350 to clamp and fix the reagent tube, and squeezes the compression spring 340 during the sliding process, so that the compression spring 340 is compressed and stored;

[0096] When removing the reagent tube, remove the cover 510 from the top opening of the box 100, the first magnet 610 and the second magnet 620 will disconnect the magnetic force, and the compression spring 340 will push the clamping plate 310 and the sliding block 330 away from the reagent tube, releasing the clamping of the reagent tube, and the sliding block 330 will pull the lifting plate 410 down through the elastic pull rope 350 when sliding, and then the reagent tube can be removed.

[0097] Finally, it should be noted that the above is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present utility model shall be included in the protection scope of the present utility model.

Claims

1. A constant temperature fluorescent PCR nucleic acid detection kit for foodborne Escherichia coli O157, characterized in that: include: box(100); A dividing piece (200), wherein a plurality of the dividing pieces (200) are provided and fixed at equal distances at the lower end of the interior of the box (100) and divide the reagent tubes into a plurality of groups; A clamping device (300), wherein a plurality of the clamping devices (300) are provided and are slidably mounted above the partition (200) and clamp the reagent tube; A lifting assembly (400), wherein the lifting assembly (400) is installed inside the box (100) so as to slide up and down, and is located above the clamping device (300), and when the lifting assembly (400) rises, the clamping device (300) is pulled to clamp the reagent tube; A cover plate assembly (500), wherein the cover plate assembly (500) is detachably fixed at the top opening of the box body (100); A magnetic component (600) is installed on the lifting component (400) and the cover component (500), forming a structure in which the cover component (500) pulls the lifting component (400) to rise after installation.

2. The foodborne Escherichia coli O157 constant temperature fluorescent PCR nucleic acid detection kit according to claim 1, characterized in that: The dividing piece (200) comprises: A partition plate (210), wherein two ends of the partition plate (210) are respectively fixedly connected to two end inner walls of the box body (100); A first rubber pad (220), wherein two first rubber pads (220) are provided and are respectively bonded to two sides of the partition plate (210); A sliding through hole (230), the sliding through hole (230) being formed on the partition plate (210) and penetrating vertically; A dividing block (240), wherein a plurality of dividing blocks (240) are provided and fixed at equal distances inside the sliding through hole (230).

3. The foodborne Escherichia coli O157 constant temperature fluorescent PCR nucleic acid detection kit according to claim 1, characterized in that: The clamping device (300) comprises: A clamping plate (310), the clamping plate (310) being slidably mounted inside the box body (100) and in contact with an upper surface of the partition plate (210); A second rubber pad (320), wherein the second rubber pad (320) is bonded to a side of the clamping plate (310) close to the reagent tube.

4. The foodborne Escherichia coli O157 constant temperature fluorescent PCR nucleic acid detection kit according to claim 1, characterized in that: The clamping device (300) further comprises: A sliding block (330), the sliding block (330) being fixed to the bottom of the clamping plate (310) and being slidably inserted into the interior of the sliding through hole (230); A compression spring (340), wherein two ends of the compression spring (340) are respectively fixedly connected to the sliding block (330) and the dividing block (240); An elastic pull rope (350) is fixed on the sliding block (330) and passes through the dividing block (240) to be fixedly connected to the lifting assembly (400).

5. The foodborne Escherichia coli O157 constant temperature fluorescent PCR nucleic acid detection kit according to claim 1, characterized in that: The lifting assembly (400) comprises: A lifting plate (410), the lifting plate (410) being installed inside the box (100) so as to be slidable up and down; Positioning through holes (420), wherein a plurality of the positioning through holes (420) are provided and are opened on the lifting plate (410) in a rectangular array and penetrate vertically; A rubber ring (430) is bonded to the inside of the positioning through hole (420).

6. The foodborne Escherichia coli O157 constant temperature fluorescent PCR nucleic acid detection kit according to claim 1, characterized in that: The cover plate assembly (500) comprises: A cover plate (510), wherein the cover plate (510) is detachably fixed on the top of the box body (100); A sealing gasket (520) is clamped at a connection between the cover plate (510) and the box body (100).

7. The foodborne Escherichia coli O157 constant temperature fluorescent PCR nucleic acid detection kit according to claim 1, characterized in that: The magnetic assembly (600) comprises: First magnets (610), four of which are provided and fixed at four corners of the bottom of the cover plate (510); The second magnets (620) are provided in four numbers and are fixed at the four corners of the top of the lifting plate (410) and are attracted to the first magnets (610).

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