Control device and flow cytometer
By designing control devices in flow cytometry, using thermal conductivity modules, detector modules, heat dissipation bases, temperature detection modules and thermal insulation modules, the problem of poor temperature stability of avalanche photodiodes is solved, and more accurate temperature data detection is achieved.
Patent Information
- Application Number
- CN202421194760.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-28
- Publication Date
- 2025-05-13
- Estimated Expiration
- 2034-05-28
AI Technical Summary
The temperature stability of avalanche photodiodes in existing flow cytometers is poor, resulting in insufficient accuracy in temperature detection.
A control device is designed, including a thermal conductivity module, a detector module, a heat dissipation base, a temperature detection module and a heat insulation module. The thermal conductor module realizes heat dissipation through heat transfer conductors and refrigeration sheets. The temperature detection module monitors the temperature in real time. The first thermal insulation module avoids the detector module being affected by external heat. The heat dissipation base dissipates the heat generated by the refrigeration sheets in real time.
By improving the temperature stability of the avalanche photodiode, the accuracy of temperature data detection is enhanced and the accuracy of temperature detection of the detector module is ensured.
Smart Images
Figure CN222866498U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of temperature control equipment, in particular to a control device and a flow cytometer. Background Art
[0002] Flow cytometer is a high-tech biomedical detection instrument that can quickly and accurately detect multiple physical and biological characteristics of a single cell at the same time. Flow cytometer generally includes optical system, fluid flow system, detection and data processing system, etc. The stability of the sensor APD (avalanche photodiode) in the photoelectric collection part of the flow cytometer is very important for the stable operation of the flow cytometer.
[0003] The flow cytometer in the prior art has the technical problem that the temperature stability of the avalanche photodiode is poor and the detected temperature data is not accurate enough. Utility Model Content
[0004] The utility model provides a control device and a flow cytometer, which can improve the temperature stability of an avalanche photodiode and improve the accuracy of temperature data detection of the avalanche photodiode.
[0005] The embodiments of the present invention can be implemented as follows:
[0006] An embodiment of the utility model provides a control device, which includes:
[0007] The heat conduction module comprises a heat transfer conductor and a cooling plate, wherein the cooling plate is arranged at the bottom of the heat transfer conductor;
[0008] A detector module, disposed on the heat transfer conductor and in contact with the heat transfer conductor;
[0009] A heat dissipation base is arranged below the cooling fin;
[0010] A temperature detection module, disposed on the heat transfer conductor, the temperature detection module is used to output information representing the temperature of the heat transfer conductor;
[0011] The first heat insulation module is arranged on the installation side of the detector module on the heat transfer conductor.
[0012] Optionally, at least two second thermal insulation modules are further included, the cooling plate is arranged in the middle of the bottom of the heat transfer conductor, and at least two second thermal insulation modules are arranged between the heat conduction module and the heat dissipation base and are respectively located on both sides of the cooling plate.
[0013] Optionally, the heat transfer conductor is provided with a receiving cavity, the detector module is installed in the receiving cavity, and the surface of the detector module is in contact with the inner wall of the receiving cavity.
[0014] Optionally, a heat-conducting layer is provided on the inner wall of the accommodating cavity.
[0015] Optionally, the detector module includes at least one avalanche photodiode, and the number of the avalanche photodiodes corresponds to the number of the accommodating cavities.
[0016] Optionally, the control device further comprises a signal board, which is arranged on a side of the first thermal insulation module away from the thermal conductive module, the pin of the avalanche photodiode is connected to the signal board, and the signal board is used to convert the current signal of the avalanche photodiode into a voltage signal.
[0017] Optionally, the control device further includes a chip protection shielding plate, and the chip protection shielding plate is arranged between the signal board and the first thermal insulation module.
[0018] Optionally, it also includes a voltage regulating module, which includes a connected voltage regulating circuit and a bias power supply, the bias power supply is connected to the signal board and the temperature detection module, the signal board is used to transmit the converted voltage signal of the avalanche photodiode to the bias power supply, the bias power supply is used to obtain the temperature information detected by the temperature detection module and adjust the operating voltage according to the temperature information, and the voltage regulating circuit outputs the operating voltage to the detector module.
[0019] Optionally, a detector control board and a TEC control board are further included, wherein the detector control board and the TEC control board are both arranged on a side of the signal board away from the avalanche photodiode, and the TEC control board is connected to the thermal conductive module.
[0020] The embodiment of the utility model further provides a flow cytometer, comprising a housing and a control device, wherein the control device is installed in the housing.
[0021] The beneficial effects of the control device and flow cytometer of the embodiment of the utility model include, for example:
[0022] The control device includes a heat conduction module, a detector module, a heat dissipation base, a temperature detection module and a first heat insulation module. The heat conduction module includes a heat transfer conductor and a cooling plate. The cooling plate is arranged at the bottom of the heat transfer conductor. The detector module is arranged on the heat transfer conductor and contacts the heat transfer conductor. The heat dissipation base is arranged below the cooling plate. The temperature detection module is arranged on the heat transfer conductor, and the temperature detection module is used to output information representing the temperature of the heat transfer conductor. The first heat insulation module is arranged on the installation side of the detector module on the heat transfer conductor. When the control device is in use, the temperature detection module is used to output information representing the temperature of the heat transfer conductor. The first heat insulation module is arranged on the installation side of the detector module on the heat transfer conductor, so as to avoid the heat of the control module on the installation side of the detector module affecting the detector module and the heat transfer conductor, thereby causing the temperature data detected by the temperature detection module to be inaccurate. The heat dissipation base is arranged below the cooling plate, so as to dissipate the heat generated when the cooling plate is working in real time. In summary, the control device can reduce the influence of the ambient temperature on the detector module and the heat transfer conductor, improve the temperature control accuracy, and thus ensure the accuracy of the temperature detection of the detector module.
[0023] The flow cytometer comprises a housing and a control device, wherein the control device is installed in the housing. The flow cytometer has all the beneficial effects of the above control device. BRIEF DESCRIPTION OF THE DRAWINGS
[0024] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the utility model and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.
[0025] Figure 1 A schematic diagram of the structure of the control device provided by this embodiment from a first perspective;
[0026] Figure 2 A schematic diagram of the structure of the control device provided by this embodiment from a second perspective;
[0027] Figure 3 A schematic diagram of the structure of the control device provided in this embodiment from a third perspective.
[0028] Icons: 10-heat conduction module; 11-heat transfer conductor; 111-accommodation cavity; 12-refrigeration plate; 13-TEC control board; 20-temperature detection module; 30-detector module; 40-heat dissipation base; 50-first thermal insulation module; 60-second thermal insulation module; 70-signal board; 80-chip protection shielding plate; 90-detector control board; 100-control device. DETAILED DESCRIPTION
[0029] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Generally, the components of the embodiments of the utility model described and shown in the drawings here can be arranged and designed in various different configurations.
[0030] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the present invention to be protected, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0031] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.
[0032] In the description of the present utility model, it should be noted that if the terms "upper", "lower", "inside", "outside", etc. appear, the orientation or position relationship indicated is based on the orientation or position relationship shown in the accompanying drawings, or is the orientation or position relationship in which the utility model product is usually placed when used. It is only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it cannot be understood as a limitation on the present utility model.
[0033] In addition, the terms “first”, “second”, etc., if used, are merely used to distinguish between the descriptions and should not be understood as indicating or implying relative importance.
[0034] It should be noted that, in the absence of conflict, the features in the embodiments of the present invention may be combined with each other.
[0035] Flow cytometer is a high-tech biomedical detection instrument that can quickly and accurately detect multiple physical and biological characteristics of a single cell at the same time. Flow cytometer generally includes optical system, fluid flow system, detection and data processing system, etc. The stability of the sensor APD (avalanche photodiode) in the photoelectric collection part of the flow cytometer is very important for the stable operation of the flow cytometer.
[0036] The flow cytometer in the related art has the technical problem that the temperature stability of the avalanche photodiode is poor and the detected temperature data is not accurate enough.
[0037] Please refer to Figure 1-Figure 3This embodiment provides a flow cytometer, which includes a housing and a control device 100, wherein the control device 100 is installed in the housing. The flow cytometer can effectively improve the above-mentioned technical problems, improve the temperature stability of the avalanche photodiode, and improve the accuracy of the avalanche photodiode temperature data detection.
[0038] The control device 100 includes a heat conduction module 10, a detector module 30, a heat dissipation base 40, a temperature detection module 20 and a first heat insulation module 50. The heat conduction module 10 includes a heat transfer conductor 11 and a cooling plate 12. The cooling plate 12 is arranged at the bottom of the heat transfer conductor 11. The detector module 30 is arranged on the heat transfer conductor 11 and contacts the heat transfer conductor 11. The heat dissipation base 40 is arranged below the cooling plate 12. The cooling plate 12 generates heat when performing cooling or heating work. The generated heat can be dissipated in real time through the heat dissipation base 40, thereby achieving Rapid heat dissipation; the temperature detection module 20 is arranged on the heat transfer conductor 11, and the temperature detection module 20 is used to output information representing the temperature of the heat transfer conductor 11; the first thermal insulation module 50 is arranged on the installation side of the detector module 30 on the heat transfer conductor 11, and the detector module 30 can be installed on the installation side of the heat transfer conductor 11 through the first thermal insulation module 50. At the same time, the first thermal insulation module 50 can prevent the heat of the control module on the installation side of the detector module 30 from affecting the detector module 30 and the heat transfer conductor 11, thereby ensuring that the detected temperature of the heat transfer conductor 11 is more accurate.
[0039] In the present embodiment, the detector module 30 includes at least one avalanche photodiode. The temperature detection module 20 can monitor the actual temperature of the avalanche photodiode arranged on the thermal conductive module 10 in real time, and dissipate heat through the thermal conductive module 10. In the present embodiment, the number of avalanche photodiodes is six. In other embodiments, the number of avalanche photodiodes can be increased or decreased, and is not specifically limited here. In the present embodiment, the heat transfer conductor 11 is a long strip structure, and the temperature detection module 20 is arranged at the end of the heat transfer conductor 11. Among them, the temperature detection module 20 is arranged at a position where the end of the heat transfer conductor 11 is close to the top surface.
[0040] It can be understood that the heat transfer conductor 11 is provided with a receiving cavity 111 , the detector module 30 is installed in the receiving cavity 111 , and the surface of the detector module 30 is in contact with the inner wall of the receiving cavity 111 .
[0041] In this embodiment, the number of avalanche photodiodes corresponds to the number of accommodating cavities 111. The shape of the accommodating cavity 111 matches the shape of the avalanche photodiode, and is specifically a circular structure.
[0042] More preferably, the inner wall of the accommodating cavity 111 is provided with a heat-conducting layer, and the heat-conducting layer can better conduct the temperature of the avalanche photodiode. Specifically, the heat-conducting layer can be a heat-conducting glue. In other embodiments, the heat-conducting layer can also be a heat-conducting silicone grease.
[0043] Furthermore, the heat conduction module 10 in the prior art is prone to the problem of heat backflow when dissipating heat. In order to solve this technical problem, in the present embodiment, the heat conduction module 10 further includes at least two second heat insulation modules 60, the cooling sheet 12 is arranged in the middle of the bottom of the heat transfer conductor 11, and at least two second heat insulation modules 60 are arranged between the heat conduction module 10 and the heat dissipation base 40, and are respectively arranged on both sides of the cooling sheet 12, wherein the cooling sheet 12 is respectively connected to the heat transfer conductor 11 and the heat dissipation base 40 through the second heat insulation modules 60. Specifically, the second heat insulation modules 60 are arranged on both sides of the bottom surface of the cooling sheet 12, so as to block the heat that enters the lower heat dissipation base 40 or the side outside from being transferred back to the cooling sheet 12 again, thereby avoiding the phenomenon of heat backflow during the heat dissipation process and improving the overall heat dissipation effect.
[0044] In this embodiment, the first insulation module 50 and the second insulation module 60 are both alumina ceramics. In other embodiments, the first insulation module 50 and the second insulation module 60 can also be glass insulation wool, air insulation wool, etc., which are not specifically limited here.
[0045] In this embodiment, there are two second heat insulation modules 60. In other embodiments, the number of the second heat insulation modules 60 can be adjusted according to actual conditions, and is not specifically limited here.
[0046] It should be noted that the control device 100 also includes a signal board 70 and a chip protection shielding board 80. The signal board 70 is arranged on the side of the first thermal insulation module 50 away from the thermal conductive module 10. The pin of the avalanche photodiode is connected to the signal board 70. The signal board 70 is used to convert the current signal of the avalanche photodiode into a voltage signal; the chip protection shielding board 80 is arranged between the signal board 70 and the first thermal insulation module 50. The chip protection shielding board 80 can facilitate the installation of the signal board 70 and the first thermal insulation module 50, and can also prevent external factors from affecting the circuit signal of the signal board 70. The first thermal insulation module 50 can block the temperature of the signal board 70 from being transmitted to the heat transfer conductor 11.
[0047] It should also be explained that the thermal conductive module 10 also includes a TEC control board 13, which is arranged on a side of the signal board 70 away from the avalanche photodiode, and the TEC control board 13 is connected to the cooling plate 12, and the cooling plate 12 is temperature-controlled by the TEC control board 13. Among them, the TEC control board 13 includes a drive control module and a temperature monitoring circuit, the temperature monitoring circuit is connected to the drive control module, and the temperature monitoring circuit is connected to the heat transfer conductor 11, and is used to monitor the temperature information of the heat transfer conductor 11 in real time and feed it back to the drive control module, and the drive control module controls the cooling plate 12 to cool or heat according to the difference between the actual temperature information fed back by the temperature monitoring circuit and the target control temperature information.
[0048] Furthermore, the control module further includes a detector control board 90 , which is disposed on a side of the signal board 70 away from the avalanche photodiode and is connected to the detector module 30 . The detector control board 90 is used to control and adjust the detector module 30 .
[0049] It should also be explained that the control device 100 also includes a voltage regulating module, which includes a connected voltage regulating circuit and a bias power supply. The bias power supply is connected to the signal board 70 and the temperature detection module 20. The signal board 70 is used to transmit the converted voltage signal of the avalanche photodiode to the bias power supply. The bias power supply is used to obtain the temperature information detected by the temperature detection module 20 and adjust the working voltage according to the temperature information. The voltage regulating circuit outputs the working voltage to the detector module 30. Specifically, the voltage regulating module can obtain the temperature information fed back by the temperature detection module 20. The voltage regulating module outputs the working voltage to the avalanche photodiode according to the difference between the temperature information and the target temperature information, thereby compensating the avalanche photodiode and ensuring the working stability of the avalanche photodiode.
[0050] In this embodiment, the output end of the voltage regulating circuit is connected to the working pin of the avalanche photodiode.
[0051] Specifically, when the temperature information exceeds the target temperature information, the bias power supply outputs a smaller operating voltage to the voltage regulating circuit, and the voltage regulating circuit outputs the smaller operating voltage to the detector module 30; when the temperature information does not reach the target temperature information, the bias power supply outputs a larger operating voltage to the voltage regulating circuit, and the voltage regulating circuit outputs the larger operating voltage to the detector module 30.
[0052] Specifically, when the actual temperature information exceeds the target control temperature information, the driving control module controls the refrigeration fins 12 to cool; when the actual temperature information does not reach the target control temperature information, the driving control module controls the refrigeration fins 12 to heat.
[0053] In addition, the heat conduction module 10 also includes a heat conduction component, and the top of the refrigeration plate 12 is provided with a heat conduction component. Optionally, the bottom of the refrigeration plate 12 is also provided with a heat conduction component. The heat conduction component is arranged in the middle position of the top and / or bottom of the refrigeration plate 12. Specifically, the heat conduction component is thermal conductive silicone grease, and the thermal conductive silicone grease is applied to the top and bottom surfaces of the refrigeration plate 12. Thereby ensuring that the heat of the heat transfer conductor 11 can be quickly dissipated through the heat dissipation base 40. At the same time, the thermal conductive silicone grease can also reduce the temperature difference between the top and bottom surfaces of the refrigeration plate 12, thereby improving the working efficiency of the refrigeration plate 12.
[0054] In other embodiments, the heat-conducting component may also be a heat-conducting adhesive. The heat-conducting adhesive may be disposed on other positions of the top surface and the bottom surface of the cooling plate 12, which is not specifically limited here.
[0055] The control device 100 and flow cytometer provided in this embodiment have at least the following advantages:
[0056] The avalanche photodiode of the flow cytometer in the prior art can receive and amplify fluorescence signals of different wavelengths. The amplified signal is received and processed by the acquisition card and uploaded to the host computer for data analysis to finally generate the result. The working stability of the avalanche photodiode is very important for the overall stability of the flow cytometer. Among them, the factors affecting the stability of the avalanche photodiode include reverse voltage and ambient temperature. Therefore, ensuring the stability of the reverse voltage and the temperature is the key to ensuring the stability of the avalanche photodiode. When the control device 100 provided in this embodiment is in use, the temperature detection module 20 is used to output information characterizing the temperature of the heat transfer conductor 11. Since the temperature stability of the detector module 30 is easily affected by the external temperature, the first heat insulation module 50 is set on the installation side of the detector module 30 on the heat transfer conductor 11. The detector module 30 is fixed in the heat transfer conductor 11 through the first heat insulation module 50, and the temperature of the detector module 30 can be prevented from being affected by the outside, so that the temperature data of the avalanche photodiode detected by the temperature detection module 20 is not accurate enough, and a heat dissipation base 40 is set below the cooling plate 12, so as to dissipate the heat generated when the cooling plate 12 is working in real time. The control device 100 can isolate the temperature of the detector module 30 from the influence of the external temperature through the first heat insulation module 50 .
[0057] In summary, the embodiment of the utility model provides a control device 100 and a flow cytometer, the control device 100 includes a heat conduction module 10, a detector module 30, a heat dissipation base 40, a temperature detection module 20 and a first thermal insulation module 50, the heat conduction module 10 includes a heat transfer conductor 11 and a cooling plate 12, the cooling plate 12 is arranged at the bottom of the heat transfer conductor 11, the detector module 30 is arranged on the heat transfer conductor 11 and contacts with the heat transfer conductor 11; the heat dissipation base 40 is arranged below the cooling plate 12; the temperature detection module 20 is arranged on the heat transfer conductor 11, and the temperature detection module 20 is used to output information characterizing the temperature of the heat transfer conductor 11; the first thermal insulation module 50 is arranged on the installation side of the detector module 30 on the heat transfer conductor 11. When the control device 100 is in use, the temperature detection module 20 is used to output information representing the temperature of the heat transfer conductor 11. Since the temperature of the detector module 30 is easily affected by the external temperature, the first heat insulation module 50 is set on the installation side of the detector module 30 on the heat transfer conductor 11, so as to avoid the heat of the control module on the installation side of the detector module 30 affecting the detector module 30 and the heat transfer conductor 11, thereby causing the temperature data detected by the temperature detection module 20 to be inaccurate, and a heat dissipation base 40 is set under the cooling plate 12, so as to dissipate the heat generated by the cooling plate 12 when it is working in real time. The control device 100 can block the heat of the control module on the installation side of the detector module 30 from affecting the detector module 30 and the heat transfer conductor 11 through the first heat insulation module 50, ensure the stability of the temperature of the detector module 30, and thus ensure the accuracy of the temperature detection of the detector module 30.
[0058] The flow cytometer comprises a housing and a control device 100, wherein the control device 100 is installed in the housing. The flow cytometer has all the beneficial effects of the control device 100.
[0059] The above is only a specific implementation of the utility model, but the protection scope of the utility model is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed by the utility model should be included in the protection scope of the utility model. Therefore, the protection scope of the utility model should be based on the protection scope of the claims.
Claims
1. A control device, characterized in that: include: A heat conduction module (10) comprises a heat transfer conductor (11) and a cooling fin (12), wherein the cooling fin (12) is arranged at the bottom of the heat transfer conductor (11); A detector module (30) is disposed on the heat transfer conductor (11) and is in contact with the heat transfer conductor (11); A heat dissipation base (40) is arranged below the cooling fin (12); A temperature detection module (20) is arranged on the heat transfer conductor (11), and the temperature detection module (20) is used to output information representing the temperature of the heat transfer conductor (11); A first heat insulation module (50) is arranged on the installation side of the detector module (30) on the heat transfer conductor (11).
2. The control device according to claim 1, characterized in that: It also includes at least two second thermal insulation modules (60), the cooling fin (12) is arranged in the middle of the bottom of the heat transfer conductor (11), and at least two of the second thermal insulation modules (60) are arranged between the heat conduction module (10) and the heat dissipation base (40), and are respectively located on both sides of the cooling fin (12).
3. The control device according to claim 1, characterized in that: The heat transfer conductor (11) is provided with a receiving cavity (111), the detector module (30) is installed in the receiving cavity (111), and the surface of the detector module (30) is in contact with the inner wall of the receiving cavity (111).
4. The control device according to claim 3, characterized in that: The inner wall of the accommodating cavity (111) is provided with a heat-conducting layer.
5. The control device according to claim 3, characterized in that: The detector module (30) comprises at least one avalanche photodiode, and the number of the avalanche photodiodes corresponds to the number of the accommodating cavities (111) in a one-to-one manner.
6. The control device according to claim 5, characterized in that: The control device (100) further comprises a signal board (70), wherein the signal board (70) is arranged on a side of the first thermal insulation module (50) away from the thermal conductive module (10), the pins of the avalanche photodiode are connected to the signal board (70), and the signal board (70) is used to convert the current signal of the avalanche photodiode into a voltage signal.
7. The control device according to claim 6, characterized in that: The control device (100) further comprises a chip protection shielding plate (80), wherein the chip protection shielding plate (80) is arranged between the signal plate (70) and the first heat insulation module (50).
8. The control device according to claim 6, characterized in that: It also includes a voltage regulating module, the voltage regulating module including a voltage regulating circuit and a bias power supply connected to each other, the bias power supply is connected to the signal board (70) and the temperature detection module (20), the signal board (70) is used to transmit the converted voltage signal of the avalanche photodiode to the bias power supply, the bias power supply is used to obtain the temperature information detected by the temperature detection module (20) and adjust the working voltage according to the temperature information, and the voltage regulating circuit outputs the working voltage to the detector module (30).
9. The control device according to claim 8, characterized in that: It also includes a detector control board (90) and a TEC control board (13), wherein the detector control board (90) and the TEC control board (13) are both arranged on a side of the signal board (70) away from the avalanche photodiode, and the TEC control board (13) is connected to the thermal conductive module (10).
10. A flow cytometer, characterized in that: It comprises a housing and the control device according to any one of claims 1 to 9, wherein the control device (100) is installed in the housing.