Low-temperature preservation box for intestinal stem cells

Abstract

The utility model relates to a preservation box, in particular to a low-temperature preservation box for intestinal stem cells. The utility model provides an intestinal stem cell cryopreservation box, including seal box, seal cover, bump, buckle, fixture block, ventilation frame and refrigerator etc. The seal box is rotatingly connected with the seal cover, the seal cover is fixedly connected with the bump, the front part of the seal cover is symmetrically and fixedly connected with the buckle, the front part of the seal box is symmetrically and fixedly connected with the fixture block. A ventilation frame is fixedly placed in the sealing box, an interlayer is arranged between the ventilation frame and the sealing box, a plurality of ventilation holes are formed in the four faces of the ventilation frame, and a refrigerator is fixedly placed on the ventilation frame. The refrigerator is fixedly installed at the bottom in the ventilation frame, cold air reaches the interlayer from the ventilation holes to enable the periphery of the storage frame to be cooled evenly, and therefore the effect that intestinal stem cells are prevented from deteriorating easily at the normal temperature, and the survival rate of the intestinal stem cells is reduced is achieved.

Description

Technical Field

[0001] The utility model relates to a preservation box, in particular to a low-temperature preservation box for intestinal stem cells. Background Art

[0002] Intestinal stem cells are the source of various types of intestinal cells, providing new vitality to keep the intestine fresh and energetic. In the intestine, there are some invaginations between the protruding intestinal villi, called crypts. Intestinal stem cells are located at the base of the intestinal mucosal crypts, that is, the basal crypts are the cell bank of intestinal stem cells. Intestinal stem cells play an important role in intestinal renewal and maintenance, and are closely related to the pathogenic mechanism of many diseases. In the prior art, intestinal stem cells are usually separated and cultured from different tissues, then tested and identified, and then frozen at low temperatures so that the intestinal stem cells can be revived and used in time when needed.

[0003] After medical staff extract intestinal stem cells from tissues into test tubes, they usually do not test them immediately. However, in order to improve the survival rate and freshness of the cells, they will be temporarily stored in a preservation box. After testing, they will be placed in a refrigerator at -80°C. Preservation boxes on the market usually do not have the function of low-temperature preservation. Intestinal stem cells are prone to breed bacteria at room temperature, causing intestinal stem cells to deteriorate easily and reducing the survival rate of intestinal stem cells. The temperature can be lowered to slow down the growth rate of bacteria in intestinal stem cells and extend the shelf life of intestinal stem cells, thereby preventing intestinal stem cells from deteriorating easily at room temperature and reducing the survival rate of intestinal stem cells.

[0004] Therefore, it is necessary to design an intestinal stem cell low-temperature preservation box to solve the above technical problems. Utility Model Content

[0005] In order to overcome the disadvantages that the preservation boxes on the market usually do not have the function of low-temperature preservation, and intestinal stem cells are prone to breed bacteria at room temperature, causing intestinal stem cells to deteriorate easily, the technical problem of the utility model is to provide an intestinal stem cell low-temperature preservation box.

[0006] The technical solution is: a low-temperature preservation box for intestinal stem cells, including a sealed box, a sealed cover, a protrusion, a buckle, a block, a ventilation rack, a refrigerator, a storage frame and a handle. The sealed box is rotatably connected to the sealed cover, the sealed cover is fixedly connected to the protrusion, the front of the sealing cover is symmetrically fixedly connected to the buckle, the front of the sealed box is symmetrically fixedly connected to the block, each buckle is clamped with each block, a ventilation rack is fixedly placed in the sealed box, a mezzanine is provided between the ventilation rack and the sealed box, a plurality of ventilation holes are provided on four surfaces of the ventilation rack, a refrigerator is fixedly placed on the ventilation rack, blocks are fixedly provided on both sides of the inner wall of the ventilation rack, the blocks divide the ventilation rack into two layers, an upper and lower layer, a storage frame is placed on the upper layer of the ventilation rack, a storage frame is fixedly connected to the lower layer of the ventilation rack, each storage frame is provided with a plurality of placement slots, and handles are fixedly connected on both sides of the top of the storage frame located on the upper layer of the ventilation rack.

[0007] In a preferred embodiment of the utility model, a U-shaped rod is further included. A square groove is formed on the protrusion, and the U-shaped rod is rotatably connected in the square groove of the protrusion.

[0008] In a preferred embodiment of the utility model, a leaf spring and a spring are also included. A plurality of leaf springs are slidably connected in an annular array on the inner wall of the placement slot of each storage frame, and a spring is connected between each leaf spring and the placement slot on the same side.

[0009] In a preferred embodiment of the utility model, a controller is also included. The front part of the sealed box is fixed with a controller that can control the switch and adjust the displayed temperature. The controller is electrically connected to the refrigerator.

[0010] In a preferred embodiment of the utility model, an anti-skid pad is also included, and the four corners of the bottom of the sealed box and each storage frame are fixedly connected with the anti-skid pad.

[0011] In a preferred embodiment of the utility model, a groove is provided at the bottom of the sealing box, and the protrusion at the top of the sealing cover is engaged with the groove at the bottom of another sealing box.

[0012] The utility model has the following advantages: 1. The utility model fixes a refrigerator at the bottom of the ventilation frame, and cold air reaches the interlayer from the ventilation holes to cool the surrounding area of ​​the storage frame evenly, thereby preventing intestinal stem cells from easily deteriorating at room temperature and reducing the survival rate of intestinal stem cells.

[0013] 2. The utility model arranges leaf springs on the inner wall of the placement groove, so that different test tubes can be stably fixed in the placement groove, thereby preventing the test tubes from shaking and causing rupture.

[0014] 3. The utility model achieves the effect of observing and detecting intestinal stem cells of different degrees by installing a controller on the front side of the sealed box.

[0015] 4. The utility model provides an anti-skid pad at the bottom of the sealed box, which increases friction, so that the sealed box is firmly placed on the workbench and is not easy to slide accidentally. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a structural schematic diagram of the utility model.

[0017] Figure 2 It is a partial cross-sectional structural diagram of the sealing box, sealing cover, protrusion and other components of the utility model.

[0018] Figure 3 It is a structural schematic diagram of a partial section of the sealing cover, the convex block, the U-shaped rod and other components of the utility model.

[0019] Figure 4 It is a structural schematic diagram of a partial section of the ventilation rack, storage frame, handle and other components of the utility model.

[0020] Figure 5 It is a schematic diagram of the top structure of the storage frame, handle and leaf spring of the utility model.

[0021] Figure 6 It is a structural schematic diagram of a partial section of the handle, leaf spring, spring and other components of the utility model.

[0022] Figure 7 It is a schematic diagram of the structure of the controller, anti-skid pad, sealing box and other components of the utility model.

[0023] Wherein: 1-sealing box, 2-sealing cover, 3-bump, 4-U-shaped rod, 5-buckle, 6-block, 7-ventilation rack, 8-ventilation hole, 9-refrigerator, 10-storage frame, 11-handle, 12-leaf spring, 13-spring, 14-controller, 15-anti-slip pad, 16-groove. DETAILED DESCRIPTION

[0024] The technical solution of the utility model is further described below in conjunction with the accompanying drawings.

[0025] Embodiment: A low temperature storage box for intestinal stem cells, such as Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 and Figure 7As shown, it includes a sealed box 1, a sealed cover 2, a protrusion 3, a U-shaped rod 4, a buckle 5, a block 6, a ventilation rack 7, a refrigerator 9, a storage frame 10, a handle 11, a controller 14 and an anti-skid pad 15. The top of the sealed box 1 is rotatably connected to the sealed cover 2, the top of the sealed cover 2 is welded with a protrusion 3, the top of the protrusion 3 is provided with a square groove, the front side of the sealed cover 2 is symmetrically installed with buckles 5 by bolts, the front side of the sealed box 1 is symmetrically installed with blocks 6 by bolts, each buckle 5 is clamped with each block 6, the inner wall of the sealed box 1 is fixedly placed with a ventilation rack 7, a sandwich is provided between the ventilation rack 7 and the inner wall of the sealed box 1, and a plurality of ventilation racks are provided on the four sides of the ventilation rack 7 Hole 8, a refrigerator 9 is fixedly placed at the bottom of the ventilation rack 7, and blocks are welded on the inner walls on both sides of the ventilation rack 7. The blocks divide the ventilation rack 7 into two layers, an upper and lower layer, a storage frame 10 is placed on the upper layer of the ventilation rack 7, and a storage frame 10 is fixedly placed on the lower layer of the ventilation rack 7. A plurality of placement slots are opened on the top of each storage frame 10, and handles 11 are welded on the left and right sides of the top of the storage frame 10 located on the upper layer of the ventilation rack 7. A controller 14 that can control the switch and adjust the display temperature is installed on the front side of the lower part of the sealed box 1 by bolts. The controller 14 is electrically connected to the refrigerator 9, and the four corners of the bottom of the sealed box 1 and each storage frame 10 are glued with anti-slip pads 15.

[0026] When medical staff need to temporarily store intestinal stem cells at low temperature, they first place the low-temperature storage box on a horizontal workbench at a suitable position, turn the two clamping blocks 6 counterclockwise to disengage the clamping blocks 6 from the buckles 5, hold the U-shaped rod 4 and rotate it counterclockwise, then pull the U-shaped rod 4 to drive the sealing cover 2 to rotate counterclockwise to open the sealing box 1, and put the test tube containing intestinal stem cells into the placement slot in the storage frame 10. If there are many test tubes, the handle 11 can be pulled upward to take out the storage frame 10 on the upper layer of the ventilation rack 7 and place it next to it. The anti-skid pad 15 at the bottom of the storage frame 10 can increase the friction and effectively prevent slipping. When the remaining test tubes are put into the ventilation rack 7, the anti-skid pad 15 at the bottom of the storage frame 10 can increase the friction and effectively prevent slipping. The placing slot of the storage frame 10 on the lower layer of the air rack 7 is placed in the placing slot, and then the handle 11 on the storage frame 10 just taken out is held and placed in the upper layer of the ventilation rack 7, and then the U-shaped rod 4 is held and rotated clockwise, and the U-shaped rod 4 is pulled to drive the sealing cover 2 to rotate clockwise to close the sealing box 1, and then the two clamping blocks 6 are moved clockwise, and the clamping blocks 6 are engaged with the buckles 5 to form a closed space inside, that is, the placing is completed, and finally the refrigerator 9 is started through the controller 14, and the cold air emitted by the refrigerator 9 flows in the interlayer through the ventilation holes 8 on the ventilation rack 7, so that the storage frame 10 is cooled evenly to achieve the effect of improving the survival rate of intestinal stem cells; when the medical staff needs to take out the intestinal stem cells temporarily stored at low temperature When testing intestinal stem cells, the medical staff first pushes the two blocks 6 counterclockwise to disengage the blocks 6 from the buckles 5, holds the U-shaped rod 4 and rotates counterclockwise, then pulls the U-shaped rod 4 to drive the sealing cover 2 to rotate counterclockwise to open the sealing box 1, and takes out the test tube containing intestinal stem cells from the placement slot of the storage frame 10. If more test tubes need to be tested, the handle 11 can be pulled upward to take out the storage frame 10 on the upper layer of the ventilation rack 7 and place it next to it. The anti-skid pad 15 at the bottom of the storage frame 10 can increase the friction and effectively prevent slipping. After taking out the test tube from the placement slot of the lower storage frame 10 and putting it away, hold the handle 11 to put the storage frame 1 just taken out 0 is placed in the upper layer of the ventilation rack 7, and then the U-shaped rod 4 is held and rotated clockwise, and the U-shaped rod 4 is pulled to drive the sealing cover 2 to rotate clockwise to close the sealing box 1, and then the two clamping blocks 6 are moved clockwise, and the clamping blocks 6 are engaged with the buckles 5 to form a closed space inside. If all the test tubes are taken out, the refrigerator 9 is turned off through the controller 14; when it is not necessary to move the low-temperature preservation box, the anti-skid pad 15 at the bottom of the sealing box 1 increases the friction force, so that the sealing box 1 is stable on the workbench and is not easy to slide accidentally. When the medical staff needs to move the low-temperature preservation box, when it needs to be taken away, the medical staff only needs to turn out the U-shaped rod 4 counterclockwise to lift the low-temperature preservation box, and it can be taken away.

[0027] like Figure 4 , Figure 5 and Figure 6As shown, it also includes a leaf spring 12 and a spring 13. Three leaf springs 12 are slidably connected in an annular array on the inner wall of the placement groove of each storage frame 10. A spring 13 is connected between each leaf spring 12 and the placement groove on the same side. A groove 16 is opened at the bottom of the sealing box 1, and the protrusion 3 on the top of the sealing cover 2 is snap-fitted with the groove 16 at the bottom of another sealing box 1.

[0028] When the medical staff needs to clamp the test tube, the medical staff puts the test tubes of different sizes into the placement groove of the storage frame 10. The leaf spring 12 in the placement groove of the storage frame 10 is squeezed by different forces and slides in a direction away from the test tube. The spring 13 corresponding to the leaf spring 12 is squeezed and rebounds to push the leaf spring 12 to press the test tube in a direction close to the test tube, so as to clamp the test tubes of different sizes. When the environment shakes or the person shakes, the test tube placed on the placement groove of the storage frame 10 will be stuck by the leaf spring 12, so as to prevent the test tube from shaking and causing rupture. When the medical staff needs to store intestinal stem cells at different temperatures, the temperature of different levels can be adjusted on the controller 14 to achieve the effect of observing and detecting intestinal stem cells of different degrees. When the medical staff needs to put the low-temperature preservation box into the refrigerator for refrigeration, the low-temperature preservation box can be stored vertically, and the protrusions 3 and the grooves 16 of the two adjacent low-temperature preservation boxes are sequentially connected to achieve the effect of reinforced storage and not easy to be pushed over.

[0029] The technical principles of the embodiments of the present utility model are described above in combination with specific embodiments. These descriptions are only for explaining the principles of the embodiments of the present utility model, and cannot be interpreted in any way as limiting the protection scope of the embodiments of the present utility model. Based on the explanations here, technicians in this field can think of other specific implementation methods of the embodiments of the present utility model without creative work, and these methods will fall within the protection scope of the embodiments of the present utility model.

Claims

1. A low-temperature storage box for intestinal stem cells, characterized in that: The invention comprises a sealing box (1), a sealing cover (2), a protrusion (3), a buckle (5), a block (6), a ventilation rack (7), a refrigerator (9), a storage frame (10) and a handle (11). The sealing box (1) is rotatably connected to the sealing cover (2), the sealing cover (2) is fixedly connected to the buckle (5), the front of the sealing cover (2) is symmetrically fixedly connected to the block (6), each buckle (5) is clamped to each block (6), a ventilation rack (7) is fixedly placed in the sealing box (1), and the ventilation rack (7) is connected to the sealing box (1). An interlayer is arranged inside the sealed box (1), a plurality of ventilation holes (8) are provided on four surfaces of the ventilation frame (7), a refrigerator (9) is fixedly placed on the ventilation frame (7), blocks are fixedly provided on both sides of the inner wall of the ventilation frame (7), the blocks divide the ventilation frame (7) into two layers, an upper layer and an lower layer, a storage frame (10) is placed on the upper layer of the ventilation frame (7), a storage frame (10) is fixedly connected to the lower layer of the ventilation frame (7), each storage frame (10) is provided with a plurality of placement slots, and handles (11) are fixedly connected to both sides of the top of the storage frame (10) located on the upper layer of the ventilation frame (7).

2. The intestinal stem cell cryopreservation box according to claim 1, characterized in that: It also comprises a U-shaped rod (4), a square groove is provided on the convex block (3), and the U-shaped rod (4) is rotatably connected in the square groove of the convex block (3).

3. The intestinal stem cell cryopreservation box according to claim 2, characterized in that: It also includes leaf springs (12) and springs (13), wherein a plurality of leaf springs (12) are slidably connected in an annular array on the inner wall of the placement slot of each storage frame (10), and a spring (13) is connected between each leaf spring (12) and the placement slot on the same side.

4. The intestinal stem cell cryopreservation box according to claim 3, characterized in that: The invention also comprises a controller (14). The front part of the sealed box (1) is fixed with the controller (14) which can control the switch and adjust the displayed temperature. The controller (14) is electrically connected to the refrigerator (9).

5. The intestinal stem cell cryopreservation box according to claim 4, characterized in that: It also includes an anti-skid pad (15), and the four corners at the bottom of the sealed box (1) and each storage frame (10) are fixedly connected to the anti-skid pad (15).

6. The intestinal stem cell cryopreservation box according to claim 5, characterized in that: A groove (16) is provided at the bottom of the sealing box (1), and a protrusion (3) at the top of the sealing cover (2) is clamped with the groove (16) at the bottom of another sealing box (1).

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