Kit with multiple detection sites and blood analyzer for POCT (point-of-care testing)

Abstract

The utility model discloses a kit with multiple detection positions and a blood analyzer for POCT (point-of-care testing), and belongs to the technical field of POCT blood detection, the kit comprises a box body and a sealing cover, the sealing cover is arranged on the box body, a plurality of detection units are arranged in the box body, a first cavity, a second cavity, a third cavity and a microporous structure are arranged in each detection unit, and the first cavity, the second cavity, the third cavity and the microporous structure are arranged in the box body. The microporous structure is arranged between the first cavity and the second cavity, the second cavity is communicated with the third cavity, metering is started when a liquid sample enters the second cavity from the first cavity, and metering is stopped when the liquid enters the third cavity from the second cavity; the accuracy and consistency of liquid sample metering are improved, manual operation is not needed, detection of multiple liquid samples can be completed at a time, and time is saved.

Description

Technical Field

[0001] The utility model relates to the technical field of POCT blood detection, in particular to a reagent kit with multiple detection sites and a blood analyzer used for POCT. Background Art

[0002] Routine blood analyzers can perform clinical analysis and measurement of red blood cells, white blood cells, platelets and hemoglobin in the blood, so blood cell analyzers are widely used in clinical practice. Existing blood cell analyzers are based on the Coulter principle for cell counting. The analyzer includes a main control device, a liquid circuit system, a sensor detection device, a blood parameter analysis device, a display device and a cleaning device. The blood analysis process is sampling, dilution, measurement, calculation, display and cleaning in sequence. After the blood is diluted and mixed with the corresponding reagents, it is measured through the microporous channel in the detection sensor device. The measurement signal is uploaded to the analyzer for calculation, and finally the reading result is obtained; after each test, the entire liquid circuit system needs to be fully cleaned, and the components after cleaning need to be checked to see if they are clean to meet the needs of the next blood test. However, cleaning is not the core device part of the blood analyzer to complete the accurate detection of blood cells, and the cleaning procedure is time-consuming and uses a large amount of reagents, resulting in a lot of waste; in addition, the cleaning system has a complex structure, a large volume, and requires several external flushing liquid and cleaning liquid reagent barrels, which is cumbersome to operate and has a high failure rate of the instrument. A disposable blood analyzer that does not require cleaning and is easy to operate is now needed.

[0003] The existing patent application with announcement number CN216525366U discloses a test kit and a POCT blood cell analyzer. The test kit includes: a box body, a fixed plate and a side plate, wherein the box body includes a front pool, a rear pool and a through hole between the front pool and the rear pool; the fixed plate is located on the side of the front pool away from the rear pool, used to cover the front pool, and the fixed plate is provided with a front pool electrode with one end extending into the front pool; the side plate is connected to the side wall of the front pool, used to cover the rear pool, and the side plate is provided with a rear pool electrode with one end extending into the rear pool. The test kit can only detect one liquid sample at a time, and cannot automatically control the start of sample detection. Utility Model Content

[0004] One of the purposes of the utility model is to provide a reagent box with multiple detection positions, which solves the problems of low detection efficiency and inability to automatically start and stop metering of the existing reagent box.

[0005] In order to achieve the above utility model purpose, the technical solution adopted by the utility model is as follows:

[0006] A reagent kit with multiple detection sites comprises a box body and a cover, wherein the cover is arranged on the box body, and a plurality of detection units are arranged in the box body, wherein a first cavity, a second cavity, a third cavity and a microporous structure are arranged in the detection unit, and the microporous structure is arranged between the first cavity and the second cavity, and the second cavity is connected to the third cavity, and the liquid sample enters the second cavity from the first cavity to start metering, and the metering is terminated when the liquid enters the third cavity from the second cavity, thereby improving the accuracy and consistency of the liquid sample metering, automatically performing the test without manual operation, and completing the test of multiple liquid samples at one time, saving time.

[0007] Furthermore, a plurality of the detection units are connected end to end and arranged in a ring, which is convenient for sample addition and can be used in instruments with a single detection position.

[0008] Furthermore, a plurality of the detection units are arranged in a row, so as to facilitate sequential addition of liquid for use.

[0009] Furthermore, a fourth cavity is provided in the detection unit, the fourth cavity is connected to the third cavity, and the fourth cavity is used to absorb waste liquid after detection to prevent liquid leakage and contamination.

[0010] Furthermore, it also includes a first electrode, a second electrode and a third electrode. The first electrode penetrates the bottom wall of the first cavity and is arranged in the first cavity. The second electrode penetrates the bottom wall of the second cavity and is arranged in the second cavity. The third electrode penetrates the bottom wall of the third cavity and is arranged in the third cavity. It is used to transmit electrical signals for starting and ending detection, and the response is rapid, thereby improving the accuracy of detection.

[0011] Preferably, it further comprises a negative pressure port, wherein the negative pressure port is arranged in the fourth cavity, and the liquid sample is moved by the suction force generated by the negative pressure as a driving force.

[0012] Preferably, the microporous structure is a microporous sheet, a through groove is provided between the first cavity and the second cavity, the microporous sheet is embedded in the through groove, and the quantifiable object can be changed by replacing the microporous sheet, thereby expanding the use scope of the reagent kit with multiple detection positions.

[0013] Preferably, it further comprises a plug and a first through hole, wherein the first through hole is arranged above the first cavity, and the plug is clamped on the first through hole for injecting liquid samples to ensure the sealing of the box body.

[0014] Furthermore, a photoelectric detection position is also provided on the box body, and the photoelectric detection position is arranged close to the first cavity and is used for detecting the component ratio of the liquid sample by photoelectric reaction.

[0015] The second purpose of the utility model is to provide a POCT blood analyzer, which solves the problem that the existing blood analyzers cannot start and stop automatically and have low detection efficiency.

[0016] A blood analyzer for POCT includes the multi-detection site reagent kit, and reduces operation steps and consumption by replacing the multi-detection site reagent kit.

[0017] The beneficial effects of the utility model are:

[0018] (1) The box body of the multi-detection position test kit is provided with a plurality of detection units, each detection unit is independent and can independently complete the analysis of a liquid sample. The detection unit is provided with a first cavity, a second cavity and a third cavity which are connected to each other. A microporous structure is provided between the first cavity and the second cavity for Coulter counting. The second cavity and the third cavity are connected. When the detection liquid enters the second cavity from the first cavity, the reading starts, and when the detection liquid enters the third cavity from the second cavity, the counting stops. The start and stop of the detection can be accurately controlled by detecting the flow position of the liquid. The consistency and accuracy of the blood detection are high, and the operation steps when detecting multiple liquid samples can be reduced, thereby shortening the overall analysis time.

[0019] (2) The multi-detection test kit can arrange multiple detection units in a row or a ring, and can select test kits with different arrangements according to the total number of samples to be tested, thereby reducing the number of operation steps and improving the efficiency of detection, and meeting the needs of different types of analyzers; a fourth cavity for storing waste liquid is provided in each detection unit to ensure that the liquid sample does not leak out after detection and cause environmental pollution. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 An exploded view of the multi-detection site test kit provided by the utility model;

[0021] Figure 2 An axonometric diagram of a reagent kit with multiple detection positions provided by the utility model;

[0022] Figure 3 A front view of a reagent kit with multiple detection sites provided by the utility model;

[0023] Figure 4 A side view of a reagent kit with multiple detection sites provided by the utility model;

[0024] Figure 5 A bottom view of the reagent kit with multiple detection sites provided by the utility model;

[0025] Figure 6 for Figure 3 Section view along line AA;

[0026] Figure 7 for Figure 4 Sectional view along line BB;

[0027] Figure 8 for Figure 4 Sectional view along the CC line;

[0028] Fig. 9 A diagram showing the changing state of a liquid sample during the detection process of the multi-detection position test kit provided by the utility model;

[0029] Fig.10 A structural diagram of a multi-detection site kit provided in Example 2;

[0030] Fig.11 This is a structural diagram of the multi-detection site kit provided in Example 3.

[0031] Reference numerals:

[0032] 1. Box body; 11. Second through hole; 111. First electrode; 112. Second electrode; 113. Third electrode; 114. Positioning block; 115. Positioning groove; 116. Photoelectric detection position; 117. Protrusion; 12. First cavity; 13. Second cavity; 14. Third cavity; 15. Through groove; 16. Fourth cavity; 17. Liquid channel; 18. Negative pressure port; 181. Air guide column; 2. Cover; 21. First through hole; 3. Microporous structure; 4. Side cover; 5. Plug; 6. Detection unit. DETAILED DESCRIPTION

[0033] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0034] Embodiment 1

[0035] like Figure 1-Figure 9As shown, the present embodiment discloses a reagent kit with multiple detection positions, including a box body 1, a cover 2 and a plurality of detection units 6, the cover 2 is arranged on the box body 1, the plurality of detection units 6 are all arranged in the box body 1, a first cavity 12, a second cavity 13, a third cavity 14 and a microporous structure 3 are arranged in the detection unit 6, the microporous structure 3 is arranged between the first cavity 12 and the second cavity 13, the second cavity 13 is connected to the third cavity 14, a certain voltage is applied between the holes on the microporous structure 3, when the particles pass through the pores, the current changes due to the change in resistance, and the current change signal is converted into particle size data, so as to measure the particle size, when the liquid sample is injected from the first cavity 12 into the second cavity 13, the particles in the liquid sample are measured, when the liquid sample enters the third cavity 14 from the second cavity 13, the measurement of the liquid sample is terminated, the plurality of detection units 6 correspond to different liquid samples respectively, and multiple portion samples can be detected and the results can be output at one time, thereby reducing the operation steps of sample detection.

[0036] Furthermore, a fourth cavity 16 is provided in the detection unit 6, and the fourth cavity 16 is connected to the third cavity 14. The fourth cavity 16 and the third cavity 14 are connected by a liquid channel 17. The liquid channel 17 is arranged close to the cover 2, so that the liquid sample enters the fourth cavity 16 after filling the third cavity 14, thereby collecting waste liquid and preventing the liquid sample from leaking out.

[0037] Preferably, the volume of the fourth cavity 16 is greater than twice the volume of the first cavity 12 to prevent excess liquid sample from overflowing.

[0038] Furthermore, it also includes a first electrode 111, a second electrode 112 and a third electrode 113. The first electrode 111 penetrates the bottom wall of the first cavity 12 and is arranged in the first cavity 12. The second electrode 112 penetrates the bottom wall of the second cavity 13 and is arranged in the second cavity 13. The third electrode 113 penetrates the bottom wall of the third cavity 14 and is arranged in the third cavity 14. When the circuit between the first electrode 111 and the second electrode 112 is connected, the blood analyzer connects the metering circuit. When the circuit between the first electrode 111 and the third electrode 113 is connected, the blood analyzer disconnects the metering circuit, thereby obtaining metering data with high accuracy and consistency.

[0039] Preferably, a positioning block 114 is provided at the bottom of the box body 1, the positioning block 114 protrudes from the box body 1, the positioning block 114 semi-surrounds the first electrode 111, the positioning block 114 is used for bottom positioning of the reagent kit with multiple detection positions, and a positioning groove 115 is provided on the side wall of the box body 1, the positioning groove 115 is used for guiding and positioning when installing the reagent kit with multiple detection positions; in addition, a protrusion 117 is also provided at the bottom of the box body 1, the protrusion 117 is used to determine the direction in which the reagent kit is installed on the instrument body, thereby playing an anti-mistake effect.

[0040] Furthermore, it also includes a negative pressure port 18, which is arranged in the fourth cavity 16. The negative pressure port 18 sucks out the gas in the fourth cavity 16, the third cavity 14 and the second cavity 13, so that the liquid sample in the first cavity 12 flows under the action of negative pressure, providing power for the flow of the liquid sample. By controlling the amount of sucked out air, the circulation rate of the liquid sample can be controlled to ensure the accuracy and consistency of the detection.

[0041] Among them, the negative pressure formed at the negative pressure port 18 is between -10kpa and -40kpa. At the beginning of the test, a lower negative pressure is sampled, such as -5kpa to -15kpa, so that the liquid slowly enters the test cavity to avoid bubbles generated by the liquid sample being agitated during the test. After a certain period of time, the negative pressure increases, such as -25kpa to -40kpa, which can speed up the test.

[0042] Preferably, it also includes an air guide column 181, which is arranged on the bottom wall of the fourth cavity 16. Part of the air guide column 181 is in the fourth cavity 16 and close to the cover 2 to prevent the liquid sample from being sucked out, and the other part protrudes from the bottom surface of the box body 1 to facilitate positioning and connection with an external negative pressure supply device.

[0043] Preferably, the microporous structure 3 is a microporous sheet, a through groove 15 is provided between the first cavity 12 and the second cavity 13, the microporous sheet is embedded in the through groove 15, and the microporous sheet is provided with micropores for liquid samples to flow through, and the diameter of the micropores is between 50-100 μm, which is selected according to the sample to be tested.

[0044] Preferably, it also includes a plug 5 and a first through hole 21, and the first through hole 21 is arranged above the first cavity 12. Specifically, the cover 2 is provided with a first through hole 21, and the first through hole 21 is opposite to the first cavity 12 for use when adding liquid. The plug 5 is clamped on the first through hole 21 for easy assembly and cleaning.

[0045] Preferably, a side cover 4 is further included. A second through hole 11 is provided on the side wall of the box body 1. The second through hole 11 is communicated with the first cavity 12. The side cover 4 is arranged on the second through hole 11 for sealing.

[0046] Furthermore, the detection unit 6 also includes a photoelectric detection position 116, which is arranged close to the first cavity 12. Specifically, the two side walls of the first cavity 12 are arranged to be transparent for detecting light penetrating the first cavity 12 to identify the concentration of blood cells.

[0047] Preferably, the stopper 5 is made of a flexible material, which is convenient for the pipette to penetrate and add the liquid sample into the first cavity 12 , and can automatically shrink and close after the liquid sample is added.

[0048] Embodiment 2

[0049] like Fig.10 As shown, this embodiment also discloses a reagent kit with multiple detection positions, in which a plurality of detection units 6 are arranged in a row. Specifically, a plurality of detection units 6 are arranged vertically and horizontally in a grid shape, which is convenient for taking and placing and sequentially adding samples for use.

[0050] Embodiment 3

[0051] like Fig.11 The present embodiment also discloses a multi-detection site test kit, in which a plurality of detection units 6 are connected end to end and arranged in a ring, so as to facilitate sequential sample addition, and are used in conjunction with a rotatable single-detection site blood analyzer, thereby reducing the overall volume and making it convenient to carry out for use.

[0052] Embodiment 4

[0053] The present embodiment discloses a blood analyzer for POCT, including a multi-detection site reagent box and an analyzer body. The multi-detection site reagent box can be detachably connected to the analyzer body, which is convenient for quick plugging and unplugging, simplifies the operation steps of blood testing and prevents leakage of blood samples.

[0054] See also Fig. 9 , the working process of the blood analyzer is as follows:

[0055] Multiple portions of processed liquid samples are sequentially taken and added to different detection units 6 respectively. The liquid samples are added into the first cavity 12 through the first through hole 21. The reagent kit with multiple detection positions after adding the samples is installed on the analysis instrument. The positioning block 114 is aligned with the corresponding position on the blood analysis. The positioning groove 115 guides to ensure that the first electrode 111, the second electrode 112 and the third electrode 113 are connected with the corresponding electrodes. The blood analyzer is started. The blood analyzer emits light of a specific wavelength at the photoelectric detection position 116 to detect the concentration of hemoglobin. The negative pressure port 18 provides negative pressure to exhaust the air in the box body 1. The liquid in the first cavity 12 After passing through the microporous structure 3, the sample enters the second cavity 13, and the first electrode 111 and the second electrode 112 are connected in a circuit, generating a metering start signal. After the liquid sample enters the third cavity 14 from the second cavity 13, the first electrode 111 and the third electrode 113 are in contact, generating a metering termination signal. The blood analyzer calculates and processes the optical signal and the electrical signal to obtain the particle detection result of the liquid sample. After the detection is completed, the detection result is displayed on the blood analyzer. At the same time, the liquid sample enters the fourth cavity 16, and the multi-detection position test kit is removed from the blood analyzer, and then the next multi-detection position test kit can be tested.

[0056] According to the disclosure and teaching of the above description, the technical personnel in the field of the utility model can also change and modify the above implementation. Therefore, the utility model is not limited to the specific implementation methods disclosed and described above, and some modifications and changes of the utility model should also fall within the scope of protection of the claims of the utility model. In addition, although some specific terms are used in this specification, these terms are only for the convenience of description and do not constitute any limitation to the utility model.

Claims

1. A reagent kit with multiple detection sites, comprising a box body (1), a cover (2) and a plurality of detection units (6), wherein the cover (2) is arranged on the box body (1), and the plurality of detection units (6) are arranged in the box body (1), characterized in that: The detection unit (6) is provided with a first cavity (12), a second cavity (13), a third cavity (14) and a microporous structure (3); the microporous structure (3) is arranged between the first cavity (12) and the second cavity (13); the second cavity (13) is connected to the third cavity (14).

2. The multi-detection site kit according to claim 1, characterized in that: The plurality of detection units (6) are connected end to end and arranged in a ring.

3. The multi-detection site kit according to claim 1, characterized in that: A plurality of the detection units (6) are arranged in a row.

4. The multi-detection site kit according to claim 2 or 3, characterized in that: A fourth cavity (16) is also provided in the detection unit (6), and the fourth cavity (16) is communicated with the third cavity (14).

5. The multi-detection site kit according to claim 4, characterized in that: The invention also comprises a first electrode (111), a second electrode (112) and a third electrode (113); the first electrode (111) penetrates the bottom wall of the first cavity (12) and is arranged in the first cavity (12); the second electrode (112) penetrates the bottom wall of the second cavity (13) and is arranged in the second cavity (13); and the third electrode (113) penetrates the bottom wall of the third cavity (14) and is arranged in the third cavity (14).

6. The multi-detection site kit according to claim 5, characterized in that: It also includes a negative pressure port (18), and the negative pressure port (18) is arranged in the fourth cavity (16).

7. The multi-detection site kit according to claim 4, characterized in that: The microporous structure (3) is a microporous sheet, a through groove (15) is provided between the first cavity (12) and the second cavity (13), and the microporous sheet is embedded in the through groove (15).

8. The multi-detection site kit according to claim 1, characterized in that: The detection unit further comprises a plug (5) and a first through hole (21); the first through hole (21) is arranged above the first cavity (12); and the plug (5) is snap-connected to the first through hole (21).

9. The multi-detection site kit according to claim 4, characterized in that: The detection unit (6) further comprises a photoelectric detection position (116), and the photoelectric detection position (116) is arranged close to the first cavity (12).

10. A blood analyzer for POCT, characterized in that: A kit comprising the multiple detection sites according to any one of claims 1 to 9.

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