Spectral light splitting device and sample analysis equipment

Abstract

The embodiment of the utility model provides a spectrum light splitting device and sample analysis equipment, and relates to the field of optical equipment. The spectrum light splitting device comprises a light transmission optical fiber, a collimation module, a beam splitting module and a collection channel, the collimation module is used for collimating light beams passing through the light transmission optical fiber, the beam splitting module comprises at least three beam splitters arranged in the second direction, the at least three beam splitters are used for conducting or reflecting collimated light beams with different wavelengths, and each beam splitter is provided with a collection channel in a one-to-one correspondence mode; and the collection channel is used for collecting the light beams conducted or reflected by the beam splitter. The spectrum light splitting device can conduct or reflect the collimated light beams with different wavelengths through the at least three beam splitters, enables the collimated light beams with different wavelengths to be orderly transmitted to the collection channel, and can shorten the propagation stroke of the light beams and reduce the transmission loss of the light beams on the premise of ensuring the collection effect.

Description

Technical Field

[0001] The utility model relates to the field of optical equipment, in particular to a spectrum splitting device and sample analysis equipment. Background Art

[0002] Flow cytometer is a common sample analysis device. It is a biochemical instrument for rapid detection of cell particles. It is generally composed of a liquid circuit, an optical circuit, an electronic system, and a computer system. The main function of the liquid circuit is to transport the sample into the instrument for detection. The optical system generates a laser beam to irradiate the sample and collects the scattered light and fluorescence signals emitted after irradiation. The electronic system converts the optical signal into an electrical signal and transmits it to the host computer for analysis and processing. The computer system is used to control the entire instrument process and analyze the data transmitted. When the sample particles are transported to the light detection area, the fluorescent dyes they carry are irradiated by the laser beam and emit fluorescence and scattered light of different bands. Due to the different molecular structure characteristics of fluorescent dyes and different physical and chemical properties, the fluorescence wavelengths emitted by different fluorescent dyes are inconsistent, and each fluorescent dye has a strongest fluorescence emission band after being excited by a laser of a fixed wavelength. Collecting the light beam in this band can characterize the corresponding fluorescent dye. In flow cytometry applications, the particles to be detected are generally stained with a variety of different fluorescent dyes. In order to distinguish these fluorescent dyes, they are combined and paired so that the strongest emission bands produced by multiple fluorescent dyes excited by the same wavelength laser do not overlap. In this way, each emission band can be separated by spectral spectrometry.

[0003] The spectral splitter modules in existing flow cytometers all use back-and-forth reflection to separate the wavelengths of the light beam. The wide-spectrum light beam is introduced into the splitter module through an optical fiber, and the desired band is screened out by a plane mirror and a dichroic mirror or a filter. The light beam in this band is then focused into photodetectors such as PMT and APD. Due to the extra reflection from the plane mirror, the light transmission path is longer, and the light beam will gradually diverge from the original path during propagation. Therefore, some methods will add a concave mirror to correct the light beam in the light path to restore it to the originally designed transmission path, thereby increasing the complexity of the entire light path and increasing the difficulty of alignment. Utility Model Content

[0004] The utility model provides a spectrum splitting device and a sample analysis device, which can shorten the propagation distance of a light beam, reduce the light beam transmission loss, and further reduce the complexity of the light path, thereby reducing the difficulty of processing and aligning optical elements.

[0005] The embodiments of the present invention can be implemented as follows:

[0006] The embodiment of the utility model provides a spectrum splitting device, which includes:

[0007] A light transmission optical fiber, a collimation module, a beam splitting module, and a collection channel are sequentially arranged along a first direction;

[0008] Among them, the collimation module is used to collimate the light beam passing through the light transmitting optical fiber, and the beam splitting module includes at least three beam splitters arranged along the second direction, and the at least three beam splitters are used to conduct or reflect collimated light beams of different wavelengths. Each beam splitter is provided with a collection channel in a one-to-one correspondence, and the collection channel is used to collect the light beam after being conducted or reflected by the beam splitter.

[0009] Optionally, the beam splitter is tilted, and an angle α is formed between an extension direction of the beam splitter and the first direction, wherein 40°≤α≤50°.

[0010] Optionally, the spectral spectrometer further includes a reflector, the reflector is correspondingly provided with the collecting channel, and the reflector is used to receive the light beam conducted by the beam splitter and reflect it to the corresponding collecting channel.

[0011] Optionally, the collimating module is a doublet lens or a first single lens.

[0012] Optionally, the collecting channel includes a filtering module, a one-way transmission module and a receiving module arranged in sequence along the first direction, the filtering module is used to filter the light beam conducted or reflected by the beam splitting module, and the one-way transmission module is used to focus the light beam filtered by the filtering module and transmit it to the receiving module.

[0013] Optionally, the filter module includes at least three filters arranged along the second direction, the at least three filters correspond to the at least three beam splitters one by one, and the filters are used to filter the light beams conducted or reflected by the beam splitting module.

[0014] Optionally, the single-lens module includes at least three second single lenses arranged along the second direction, each of the collecting channels has the second single lens, and the second single lens is used to focus the light beam filtered by the filter module.

[0015] Optionally, the receiving module includes at least three receiving target surfaces arranged along the second direction, each of the collecting channels has the receiving target surface, and the receiving target surfaces are respectively used to receive the light beams focused by the single-transmission module. The receiving target surfaces are arranged on a detector, and the detector is used to convert the optical signal into an electrical signal.

[0016] An embodiment of the utility model further provides a sample analysis device, comprising the spectrum splitting device.

[0017] The beneficial effects of the spectral spectrometer and sample analysis device of the present utility model include, for example:

[0018] The spectral spectrometer includes a light transmission fiber, a collimation module, a beam splitting module and a collection channel arranged in sequence along a first direction; wherein the collimation module is used to collimate the light beam passing through the light transmission fiber, and the beam splitting module includes at least three beam splitters arranged along a second direction, and at least three beam splitters are used to conduct or reflect collimated light beams of different wavelengths, and each beam splitter is provided with a collection channel in a one-to-one correspondence, and the collection channel is used to collect the light beams conducted or reflected by the beam splitter. In the process of light beam collection, the spectral spectrometer can conduct or reflect the collimated light beams of different wavelengths through at least three beam splitters, and make the collimated light beams of different wavelengths be transmitted to the collection channel in an orderly manner. On the premise of ensuring the collection effect, the propagation distance of the light beam can be shortened, the light beam transmission loss can be reduced, and then the complexity of the optical path can be reduced, thereby reducing the processing difficulty and alignment difficulty of the optical element.

[0019] The sample analysis device comprises a spectrum spectrometer, which has all the functions of a spectrum spectrometer. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the utility model and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

[0021] Figure 1 It is a schematic structural diagram of the spectrum splitting device provided in the embodiment of the utility model.

[0022] Icons: 100-spectral spectrometer; 110-light transmission optical fiber; 120-collimation module; 130-beam splitting module; 131-beam splitter; 140-filter module; 141-filter; 150-single-transmission module; 151-single lens; 160-receiving module; 161-receiving target surface; 170-collecting channel; 180-reflector; 181-reflection layer. DETAILED DESCRIPTION

[0023] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Generally, the components of the embodiments of the utility model described and shown in the drawings here can be arranged and designed in various different configurations.

[0024] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the present invention to be protected, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0026] In the description of the present utility model, it should be noted that if the terms "upper", "lower", "inside", "outside", etc. appear, the orientation or position relationship indicated is based on the orientation or position relationship shown in the accompanying drawings, or is the orientation or position relationship in which the utility model product is usually placed when used. It is only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it cannot be understood as a limitation on the present utility model.

[0027] In addition, the terms “first”, “second”, etc., if used, are merely used to distinguish between the descriptions and should not be understood as indicating or implying relative importance.

[0028] The terms "comprises," "comprising," or any other variation thereof are intended to encompass non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements, but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus. In the absence of further limitations, an element defined by the phrase "comprising a ..." does not exclude the presence of additional identical elements in the process, method, article, or apparatus that includes the element.

[0029] Unless otherwise clearly specified and limited, the terms "disposed", "connected" and the like should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in this utility model can be understood according to specific circumstances.

[0030] It should be noted that, in the absence of conflict, the features in the embodiments of the present invention may be combined with each other.

[0031] Please refer to Figure 1The spectral spectrometry device 100 and the sample analysis equipment provided in the embodiments of the present invention can solve the above problems, which will be described in detail below.

[0032] The spectrum splitting device 100 includes a light transmission optical fiber 110, a collimation module 120, a beam splitting module 130 and a collection channel 170 arranged in sequence along a first direction;

[0033] Among them, the collimation module 120 is used to collimate the light beam passing through the light transmission optical fiber 110, and the beam splitting module 130 includes at least three beam splitters 131 arranged along the second direction, and the at least three beam splitters 131 are used to conduct or reflect collimated light beams of different wavelengths. Each beam splitter 131 is provided with a collecting channel 170 in a one-to-one correspondence, and the collecting channel 170 is used to collect the light beam after being conducted or reflected by the beam splitter 131.

[0034] During the light beam collection process, the spectral spectrometer can conduct or reflect the collimated light beams of different wavelengths through at least three beam splitters 131, and transmit the collimated light beams of different wavelengths to the collection channel 170 in an orderly manner. Under the premise of ensuring the collection effect, the propagation distance of the light beam can be shortened, the light beam transmission loss can be reduced, and the complexity of the optical path can be reduced, thereby reducing the difficulty of processing and alignment of optical components.

[0035] It is worth noting that the light transmitting optical fiber 110 refers to an optical fiber that can transmit certain special bands, which can specifically be a multimode optical fiber or a single-mode optical fiber, or a light transmitting optical fiber bundle. The specific type of the light transmitting optical fiber 110 is not limited.

[0036] Furthermore, the collimation module 120 can be a double-cemented lens or a first single lens. When the collimation module 120 is a double-cemented lens, it has a certain achromatic ability, and the collimation effect on the light beam is also better than the collimation effect of the first single lens. When the collimation module 120 is a first single lens, the collimation performance of the light beam can only be optimized under a specific narrow band of light beams, and the light beam with a wavelength far away from this specific band may cause the light beam to diverge or over-contract, thereby affecting the subsequent optical signal detection.

[0037] refer to Figure 1 , the second direction intersects with the first direction. Specifically, the second direction can also be perpendicular to the first direction.

[0038] During operation, when the first beam splitter 131 reflects a light beam of a certain wavelength, the reflected light beam can flow to the second beam splitter 131, and then the second beam splitter 131 conducts or reflects the light beam. The light beam reflected by the second beam splitter 131 will be transmitted to the filter module 140, and the light beam conducted by it will be transmitted to the third beam splitter 131.

[0039] Specifically, the beam splitter 131 can be a dichroic mirror, which is generally long-wave pass, that is, with a cut-off wavelength as the boundary, a light beam longer than the cut-off wavelength can smoothly pass through the beam splitter 131, and a light beam shorter than the cut-off wavelength is reflected by the beam splitter 131. The main arrangement of the light splitting method is that a beam with a wide spectrum comes out through the optical fiber, and generally the wavelength range of the wide spectrum beam is 420nm-850nm, which can also be expanded to 360nm-950nm, or reduced to 495-850nm and 650-850nm, and the light beam is collimated by a double glued lens to be nearly parallel beam propagation.

[0040] In addition, because a weak light signal is detected and the narrowband light beam in the longest wavelength is generally weak in intensity, by setting it in the collection channel 170 corresponding to the first beam splitter 131, the light beam propagation distance is shortened and the attenuation of light energy is avoided.

[0041] In this embodiment, the second direction can be regarded as the arrangement direction of the multiple beam splitters 131 from left to right. Among them, the wavelength of the light beam conducted by the beam splitter 131 located on the far left is the longest, and the light beam reflected by the beam splitter 131 on the far left is transmitted to the remaining beam splitters 131, and the light beams conducted by the remaining beam splitters 131 are transmitted in sequence along the second direction, and the reflected light beams are then transmitted to the collection channel 170, and the wavelengths of the light beams conducted by the remaining beam splitters 131 increase in sequence along the second direction.

[0042] Preferably, the number of the beam splitters 131 is six, and the six beam splitters 131 are equidistantly spaced. Of course, the number of the beam splitters 131 can also be three, four, seven, etc., and the specific number is not limited.

[0043] It is worth noting that in order to ensure that all light beams can be fully collected in the end, the spectral spectrometer 100 may also include a reflector 180, and the reflector 180 is provided with a corresponding collection channel 170. The reflector 180 can be arranged side by side with multiple beam splitters 131, and the reflector 180 is used to receive the light beam conducted by the beam splitter 131 located at the edge position and reflect the light beam to the corresponding collection channel 170.

[0044] In order to improve the reflection effect, the surface of the reflector 180 may be coated with a reflective layer 181. By providing the reflective layer 181, the reflector 180 can only reflect the light beam. The reflective layer 181 may be a metal layer or a reflective film layer.

[0045] refer to Figure 1 In order to reduce the difficulty of alignment, the beam splitter 131 can be tilted, and an angle α is formed between the extension direction of the beam splitter 131 and the first direction, wherein 40°≤α≤50°.

[0046] In this embodiment, the specific size of α can be 40°, 42°, 44°, 45°, 46°, 48° and 50°, etc., and there is no limitation on the specific size of α. Preferably, α=45°, in which case, the propagation direction of the reflected or conducted light beam can be controlled to remain basically stable.

[0047] refer to Figure 1 The filter module 140 includes at least three filters 141 arranged along the second direction, and the at least three filters 141 correspond to at least three beam splitters 131 one by one. The filter 141 is used to filter the light beam conducted or reflected by the beam splitter module 130, so as to obtain a light beam with a purer spectrum. It is worth noting that the filter 141 can also be set corresponding to the reflector 180, and is used to filter the light beam reflected by the reflector 180.

[0048] In this embodiment, the number of filters 141 is six, and the six filters 141 are arranged at equal intervals; of course, in other embodiments of the present invention, the number of filters 141 can also be three, four, seven, etc., and the specific number is not limited.

[0049] refer to Figure 1 The single-lens module 150 includes at least three second single lenses 151 arranged along the second direction, and each collecting channel 170 has a second single lens 151, which is used to focus the light beams of different wavelengths filtered by the light filtering module 140. Specifically, each second single lens 151 corresponds to each filter 141 one by one, so that the light beams filtered by the filter 141 can be transmitted to the corresponding second single lens 151, and the second single lens 151 is used to focus the filtered pure light beams.

[0050] In this embodiment, the number of the second single lenses 151 is six, and the six second single lenses 151 are arranged at equal intervals; of course, in other embodiments of the utility model, the number of the second single lenses 151 can also be three, four, seven, etc., and the specific number is not limited.

[0051] refer to Figure 1 The receiving module 160 includes at least three receiving target surfaces 161 arranged along the second direction. Each collecting channel 170 has a receiving target surface 161. The receiving target surfaces 161 are respectively used to receive light beams of different wavelengths after being focused by the single-head module. The receiving target surfaces 161 are set on the detector, and the detector is used to convert the optical signal into an electrical signal.

[0052] Specifically, each receiving target surface 161 corresponds to each second single lens 151, so that the light beam focused by the single lens 151 can be transmitted to the receiving target surface 161 for collection, and then the detector converts the collected light signal into an electrical signal for output. The electrical signal can be specifically expressed as a current signal, and then the current signal is used as an intermediate medium to realize the detection and processing of the light signal.

[0053] In this embodiment, the number of receiving target surfaces 161 is six, and the six receiving target surfaces 161 are arranged at equal intervals; of course, in other embodiments of the utility model, the number of receiving target surfaces 161 can also be three, four, seven, etc., and the specific number is not limited.

[0054] It should be noted that the orientation of each receiving target surface 161 in this embodiment is consistent, so that the detectors corresponding to the receiving target surface 161 are all in the same orientation, which facilitates connection with the circuit board and reduces the complexity of circuit wiring.

[0055] The embodiment of the utility model further provides a sample analysis device, which may be a flow cytometer, and includes an optical system, a liquid flow system, and a mechanical motion system, wherein the spectrum splitting device 100 is applied to the optical system.

[0056] In summary, the spectral spectrometry device 100 and the sample analysis device provided by the embodiments of the present invention have at least the following advantages:

[0057] (1) The overall device structure is compact and small, which can make the space layout utilization rate of the overall equipment higher;

[0058] (2) The light beam propagation distance is shorter, which can reduce the light transmission loss;

[0059] (3) All detectors can be arranged in the same position, which is convenient for connection with the circuit board and reduces the complexity of circuit wiring;

[0060] (4) When the retrieved light beam is a weak signal, the narrowband light beam with the longest wavelength can pass through the first beam splitter 131 and the first collecting channel 170, thereby shortening the light beam propagation distance and avoiding the attenuation of light energy.

[0061] The above is only a specific implementation of the utility model, but the protection scope of the utility model is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed by the utility model should be included in the protection scope of the utility model. Therefore, the protection scope of the utility model should be based on the protection scope of the claims.

Claims

1. A spectral spectrometer, characterized in that: include: A light transmission optical fiber (110), a collimation module (120), a beam splitting module (130), and a collection channel (170) arranged in sequence along a first direction; The collimation module (120) is used to collimate the light beam passing through the light transmission optical fiber (110), the beam splitting module (130) comprises at least three beam splitters (131) arranged along the second direction, the at least three beam splitters (131) are used to conduct or reflect collimated light beams of different wavelengths, each beam splitter (131) is provided with a collection channel (170) in a one-to-one correspondence, and the collection channel (170) is used to collect the light beam conducted or reflected by the beam splitter (131).

2. The spectral spectrometer according to claim 1, characterized in that: The beam splitter (131) is arranged tilted, and an angle α is formed between an extension direction of the beam splitter (131) and the first direction, wherein 40°≤α≤50°.

3. The spectral spectrometer according to claim 1, characterized in that: The spectral spectrometer further comprises a reflector (180), the reflector (180) being provided with the collecting channel (170) in correspondence thereto, and the reflector (180) being used to receive the light beam conducted by the beam splitter (131) and reflect the light beam to the corresponding collecting channel (170).

4. The spectral spectrometer according to claim 3, characterized in that: The surface of the reflector (180) is coated with a reflective layer (181).

5. The spectral spectrometer according to any one of claims 1 to 4, characterized in that: The collimating module (120) is a doublet lens or a first single lens.

6. The spectral spectrometer according to any one of claims 1 to 4, characterized in that: The collecting channel (170) comprises a filter module (140), a one-way transmission module (150) and a receiving module (160) which are sequentially arranged along the first direction; the filter module (140) is used to filter the light beam conducted or reflected by the beam splitting module (130); and the one-way transmission module (150) is used to focus the light beam filtered by the filter module (140) and transmit it to the receiving module (160).

7. The spectral spectrometer according to claim 6, characterized in that: The filter module (140) comprises at least three filters (141) arranged along the second direction, the at least three filters (141) corresponding one-to-one to the at least three beam splitters (131), and the filters (141) are used to filter the light beams conducted or reflected by the beam splitter module (130).

8. The spectral spectrometer according to claim 6, characterized in that: The single-lens module (150) comprises at least three second single lenses (151) arranged along a second direction, each of the collecting channels (170) has the second single lens (151), and the second single lens (151) is used to focus the light beam filtered by the filter module (140).

9. The spectral spectrometer according to claim 6, characterized in that: The receiving module (160) comprises at least three receiving target surfaces (161) arranged along the second direction, each of the collecting channels (170) has the receiving target surface (161), the receiving target surfaces (161) are respectively used to receive the light beams focused by the single-lens module (150), and the receiving target surfaces (161) are arranged on a detector, and the detector is used to convert the light signal into an electrical signal.

10. A sample analysis device, characterized in that: The invention comprises the spectral spectrometer as described in any one of claims 1 to 9.

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