Full-automatic living body biological imaging device

Abstract

The utility model discloses a full-automatic living body biological imaging device which comprises a main box body, and a mounting plate is arranged at the upper end in the main box body; an optical filter assembly is arranged on the mounting plate, and a first light shielding connecting ring is arranged at the upper end of the optical filter assembly; a lens assembly is arranged right below the optical filter assembly along the optical axis direction; an objective table for placing a living organism to be detected is arranged below the lens assembly; a light source assembly for generating exciting light is arranged above the objective table; a first Z-axis moving assembly is arranged at the upper end of the mounting plate, an X-axis moving assembly is arranged on the first Z-axis moving assembly, a first camera and a second camera are arranged on the X-axis moving assembly at intervals in the X direction, and the first camera and the second camera are used for shooting living organisms to be detected under different light wave bands and obtaining in-situ images. According to the utility model, the light shielding effect of the optical channel can be ensured on the basis that cameras with different wave bands, lenses with different magnifications and optical filters with different wave bands can be rapidly and correspondingly switched and adjusted.

Description

Technical Field

[0001] The utility model relates to the technical field of experimental imaging equipment, in particular to a fully automatic living organism imaging device. Background Art

[0002] Small animal in vivo imaging technology is a technology that uses high-sensitivity cooled CCD (Charge Coupled Device) lenses and image processing technology in conjunction with fluorescence or bioluminescence and other technical means to achieve specific tracking of molecular targets; small animal in vivo imaging technology is widely used in life sciences, medical research, and drug development.

[0003] In the in vivo biological imaging system, an excitation light of one band is irradiated into the organism to stimulate the emission light of another band emitted by the calibrated primer in the organism. The emission light image in the organism is captured by the imaging system to observe the action mode and metabolic rate of the calibrated object in the organism. In order to obtain the in situ images of the organism under different light bands during the experimental test of the in vivo biological imaging system, it is necessary to use cameras with different bands to shoot them separately. However, most of the current in vivo biological imaging systems are usually equipped with cameras of one band. When shooting and processing the organism under the corresponding light band, it is necessary to manually adjust and replace the cameras of different bands. The operation is relatively cumbersome, and the light-shielding effect of the optical channel between the camera, lens, and filter cannot be effectively guaranteed. Utility Model Content

[0004] Therefore, the technical problem to be solved by the utility model is to overcome the defects of the living organism imaging system in the prior art that, when in use, images of corresponding bands need to be collected through cameras of different bands, and cameras of different bands need to be manually adjusted and replaced, which is cumbersome to operate, and the light-shielding effect of the optical channel between the camera, lens, and filter cannot be effectively guaranteed, thereby providing a fully automatic living organism imaging device.

[0005] According to the utility model, a fully automatic living biological imaging device is provided, comprising:

[0006] The main box body has a mounting plate disposed on the upper end thereof;

[0007] A filter assembly is arranged on the mounting plate, and a first light-proof connecting ring is arranged on the upper end of the filter assembly;

[0008] A lens assembly is arranged directly below the filter assembly along the optical axis;

[0009] A stage, disposed below the lens assembly and used for placing a living organism to be tested;

[0010] A light source assembly is disposed above the stage and is used to generate excitation light;

[0011] A first Z-axis moving assembly is arranged at the upper end of the mounting plate;

[0012] An X-axis moving assembly is arranged on the first Z-axis moving assembly; a first camera and a second camera are arranged on the X-axis moving assembly at intervals along the X direction, and the first camera and the second camera are respectively used to photograph the living organism to be measured under different light bands and obtain in-situ images;

[0013] When used in an experiment, one of the light inlet holes of the first camera and the second camera is buckled with the first light-proof connecting ring.

[0014] According to the fully automatic living organism imaging device of the utility model, at least the following technical effects are achieved:

[0015] 1. The first camera and the second camera are driven to move relative to the filter assembly along the X direction at the same time through the X-axis moving component. When it is necessary to shoot the living organism to be tested on the stage under different light bands, the first camera or the second camera with the corresponding band can be automatically switched to the position just above the filter assembly along the optical axis through the X-axis moving component, so that when used in the experiment, the light source component generates excitation light of the corresponding wavelength to irradiate the living organism to be tested on the stage, and the in-situ image under the corresponding light band can be accurately obtained; the entire camera switching and adjustment process does not require manual participation, has a high degree of automation, and is easy to operate.

[0016] 2. A first Z-axis moving assembly is also provided at the upper end of the mounting plate, and the X-axis moving assembly is provided on the first Z-axis moving assembly. In the process of moving and adjusting the positions of cameras of different wavelength bands, on the one hand, the light inlet of the camera can be driven to rise and be separated from the first light-proof connecting ring by the first Z-axis moving assembly, so as to avoid the first camera and the second camera from colliding with each other and interfering with the first light-proof connecting ring and causing damage during the movement of the first camera and the second camera along the X-direction driven by the X-axis moving assembly; on the other hand, the first camera or the second camera moved to the corresponding position along the X-direction can be driven to descend by the first Z-axis moving assembly until its light inlet is engaged with the first light-proof connecting ring, so as to ensure that the optical path from the filter assembly to the camera is fully light-proof and the imaging quality is ensured; thereby, the light-proof effect of the optical channel formed between the camera, the filter assembly and the lens assembly is ensured on the basis of quickly adjusting the corresponding switching of the cameras of different wavelength bands to the position just above the filter assembly along the optical axis direction.

[0017] Preferably, a first through hole is formed through the mounting plate along the optical axis direction, a second light-shielding ring is provided at the lower end of the filter assembly, the first light-shielding ring and the second light-shielding ring are arranged concentrically along the optical axis direction, and the projection along the optical axis direction falls within the range of the first through hole; the lens assembly comprises:

[0018] A second Z-axis moving assembly is disposed on the mounting plate;

[0019] A first rotating assembly, disposed on the second Z-axis moving assembly;

[0020] A mounting plate is arranged on the first rotating assembly and driven by the first rotating assembly to rotate around the Z direction; the mounting plate is arranged in the first through hole, and a plurality of lenses with different magnifications are arranged on the mounting plate at intervals along the circumference of the mounting plate;

[0021] During the experiment, one of the light exit holes of the plurality of lenses is selectively connected to the second light-proof connecting ring.

[0022] Preferably, the filter assembly comprises:

[0023] A switching chamber is arranged at the upper end of the mounting plate, and the switching chamber and the second Z-axis moving assembly are respectively arranged on both sides of the X-axis moving assembly along the Y direction; a mounting cavity is arranged in the switching chamber; the first light-proof connecting ring and the second light-proof connecting ring are respectively arranged on the upper end surface and the lower end surface of the switching chamber, and are connected to the mounting cavity;

[0024] A rotating wheel is arranged in the installation cavity and driven by the second rotating assembly to rotate around the Z direction. A plurality of filters are arranged on the rotating wheel at intervals along the circumference of the rotating wheel, and the filters located at different circumferential positions have different wavelength bands;

[0025] When used in an experiment, one of the plurality of filters is arranged concentrically with the first light-shielding connecting ring and the second light-shielding connecting ring along the optical axis.

[0026] Preferably, a mounting hole is provided on the rotating wheel at a position corresponding to the filter, and the mounting hole passes through the rotating wheel at one end facing radially outward along the rotating wheel to form a plug-in slot, and a retaining spring is provided at one end facing radially inward along the rotating wheel. The filter comprises a connecting member and a lens, and a connecting hole is formed on the connecting member along the Z direction, and the lens is arranged in the connecting hole. The connecting member is slidably inserted in the plug-in slot and is removably locked by the retaining spring; the front end surface of the switching bin along the X direction passes through at least one position corresponding to the plug-in slot along the X direction to form a plug-in hole, and a connecting cover is detachably connected to the plug-in hole.

[0027] Preferably, each of the connecting members is provided with an RFID tag, and a RFID reading and writing module is provided at a position corresponding to one of the RFID tags on the switching bin, the RFID reading and writing module is electrically connected to a controller, and the controller is electrically connected to the second rotating assembly for rotation; the first Z-axis moving assembly, the X-axis moving assembly, the second Z-axis moving assembly and the first rotating assembly are all electrically connected to the controller;

[0028] And / or, sliding grooves are recessed on the two side walls of the plug-in slot, and sliding parts are respectively provided on the two side walls of the connector at positions corresponding to the sliding grooves;

[0029] And / or, a first magnet is disposed on the end surface of the switching chamber facing the connection cover, and a second magnet is disposed on the connection cover at a position corresponding to the first magnet, and the second magnet has opposite magnetic properties to the first magnet;

[0030] And / or, a hole position number label is provided in the plug-in slot.

[0031] Preferably, positioning members are provided one by one at the positions of the rotating wheel corresponding to the optical filters, and a positioning sensor is provided at the position of one of the positioning members in the mounting cavity, and the positioning sensor is electrically connected to the controller.

[0032] Preferably, a through hole is formed through the upper end of the switching bin along the Z direction, and the second rotating assembly comprises a second rotating motor arranged on the upper end surface of the switching bin along the Z direction, and an output end of the second rotating motor passes through the through hole and is coaxially connected to the rotating wheel;

[0033] And / or, the switching bin includes a first part and a second part which are separately arranged, the first part is arranged at the upper end of the second part, a plurality of first threaded holes are arranged at intervals on the upper end surface of the second part along the cross-sectional contour of the second part perpendicular to the Z direction, a second through hole is arranged at a position corresponding to the first threaded hole on the first part, the second through hole penetrates the first part along the Z direction, when the first part and the second part are assembled to form the switching bin, the fastening bolt passes through the second through hole and is screwed into the corresponding first threaded hole, and the end surfaces of the first part and the second part facing each other form the installation cavity.

[0034] Preferably, the second Z-axis moving assembly comprises a second height linear drive arranged on the mounting plate, the second height linear drive is provided with a connecting plate moving along the Z direction, and the first rotating assembly is provided on the side wall of the connecting plate along the Y direction toward the X-axis moving assembly;

[0035] And / or, the first rotating assembly comprises a first rotating motor arranged on the second Z-axis moving assembly along the Z direction, and an output end of the first rotating motor is coaxially connected to the mounting plate;

[0036] And / or, at least one of the lenses is configured as a long back focus lens, a mounting position is provided on the mounting plate at a position corresponding to the long back focus lens, the mounting position penetrates the mounting plate along the Z direction, the long back focus lens comprises a coaxially connected fixing ring, a connecting ring and a lens portion, the fixing ring is detachably connected in the mounting position, and one end along the Z direction is used to be buckled with the second light-shielding connecting ring, one end of the fixing ring along the Z direction away from the second light-shielding connecting ring is internally threadedly screwed with the connecting ring, and the lens portion is provided at one end of the connecting ring along the Z direction away from the fixing ring;

[0037] And / or, at least one of the lenses is configured as a short back focus lens, the short back focus lens comprises a coaxially connected fixing seat, a first lens barrel, a second lens barrel and a short back focus mirror portion, the fixing seat is detachably connected to the mounting plate, and one end along the Z direction is used to be buckled with the second light-shielding connecting ring; the first lens barrel is internally threadedly screwed on one end of the fixing seat away from the second light-shielding connecting ring along the Z direction, a relay lens is connected in the first lens barrel via a first pressing ring, and the first pressing ring is located at the end of the relay lens away from the fixing seat; the second lens barrel is provided at one end of the first lens barrel away from the fixing seat along the Z direction, and a field lens is connected in the second lens barrel via a second pressing ring; the short back focus mirror portion is provided at one end of the second lens barrel away from the first lens barrel along the Z direction.

[0038] Preferably, both sides of the mounting plate along the X direction are provided with first slide rails along the Z direction, the first Z-axis moving assembly includes a first height linear driver arranged on the mounting plate, the first height linear driver is provided with a lifting plate moving along the Z direction, the lifting plate is arranged above the mounting plate along the X direction, and both sides along the X direction are slidably connected to the first slide rails along the Z direction; the lifting plate is formed with a long strip through hole along the Z direction corresponding to the position of the first light-shielding ring, the long strip through hole is arranged along the X direction, and is used for the first light-shielding ring to be embedded and buckled with the light inlet hole of the first camera or the second camera; two second slide rails are provided at the upper end of the lifting plate, and a moving plate is slidably connected to the two second slide rails along the X direction, the first camera and the second camera are arranged on the moving plate at intervals along the X direction, and the light inlet holes of the first camera and the second camera pass through the moving plate and extend into the long strip through hole;

[0039] The X-axis moving assembly includes a main pulley and a slave pulley, the main pulley is driven to rotate by a first motor, the main pulley and the slave pulley are respectively arranged on both sides of the lifting plate along the X direction, a transmission belt is sleeved between the main pulley and the slave pulley, and the moving plate is connected to the transmission belt.

[0040] Preferably, a three-axis moving mechanism is provided at the lower end of the main box body, the loading platform is provided on the three-axis moving mechanism, and is driven by the three-axis moving mechanism to move along the X direction, the Y direction and the Z direction;

[0041] And / or, a light-proof inner container is provided in the main box;

[0042] And / or, a supporting base plate is provided at the lower end of the interior of the main box body, the gap between the supporting base plate and the inner wall of the main box body is smaller than the size of the living organism to be tested, and the sample stage is provided on the upper end surface of the supporting base plate; the mounting plate and the supporting base plate separate the interior of the main box body into an imaging chamber, an operation chamber and a motion control chamber in sequence from top to bottom along the Z direction.

[0043] Additional aspects and advantages of the present invention will be given in part in the following description, and in part will become apparent from the following description, or will be learned through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] In order to more clearly illustrate the specific implementation methods of the utility model or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0045] Figure 1 is a schematic diagram of the exploded structure of a fully automatic living organism imaging device of this embodiment;

[0046] Figure 2 This is a schematic diagram of the three-dimensional structure of a fully automatic living organism imaging device of this embodiment with the main box removed;

[0047] Figure 3 is a partial structural schematic diagram of a fully automatic living organism imaging device of this embodiment;

[0048] Figure 4 for Figure 3 Schematic diagram of the decomposition structure;

[0049] Figure 5 for Figure 3 A schematic diagram of the assembly structure of the first camera, the first Z-axis moving component and the X-axis moving component;

[0050] Figure 6 for Figure 3 A schematic diagram of the structure of the middle lens assembly;

[0051] Figure 7 for Figure 3 A schematic diagram of the structure of the middle filter assembly;

[0052] Figure 8 for Figure 7 Schematic diagram of the structure with the first part removed;

[0053] Fig. 9 for Figure 7 Schematic diagram of the decomposition structure;

[0054] Fig.10 for Fig. 9 The enlarged schematic diagram of point A in the middle;

[0055] Fig.11 for Figure 7 A structural diagram from another perspective;

[0056] Fig.12 for Figure 3 A schematic diagram of the structure of the mounting plate in FIG.

[0057] Fig.13 is a partial structural schematic diagram of the filter assembly in this embodiment;

[0058] Fig.14 Schematic diagram of the structure of the retaining spring in this embodiment;

[0059] Fig.15 is a schematic structural diagram of the rotating wheel in this embodiment;

[0060] Fig.16 is a schematic diagram of the structure of the optical filter in this embodiment;

[0061] Fig.17 Schematic diagram of the assembly structure of the three-axis moving mechanism and the stage in this embodiment;

[0062] Fig.18 Schematic diagram of the structure of the long back focus lens in this embodiment;

[0063] Fig.19 Schematic diagram of the structure of the short back focus lens in this embodiment;

[0064] Fig. 20 for Fig.19 Schematic diagram of short back-intercept relay imaging.

[0065] Description of reference numerals:

[0066] 1-main box, 11-mounting plate, 111-first through hole, 112-first slide rail, 12-light-proof inner tank, 13-switching door, 14-light-proof box door, 15-support bottom plate, 16-imaging chamber, 17-operation chamber, 18-motion control chamber;

[0067] 2-filter assembly, 21-first light-shielding ring, 22-second light-shielding ring, 23-switching chamber, 231-mounting cavity, 232-jack, 233-first magnet, 234-first part, 2341-second through hole, 235-second part, 2351-first threaded hole, 24-rotating wheel, 241-mounting hole, 2411-plug slot, 2412-slide slot, 2413-hole number label, 2414-second threaded hole, 2415-light-transmitting part, 2416-mounting part, 25-filter, 253-RFID tag, 254-RFID read-write module, 251-connector, 2511-slide part, 2512-arc part, 252-lens, 26-circlip, 261-third through hole, 27-connecting cover, 281-positioning member, 282-positioning sensor, 29-second rotating motor;

[0068] 3-lens assembly, 31-mounting plate, 311-mounting position, 32-lens, 321-fixing ring, 322-connecting ring, 323-lens part, 324-fixing seat, 325-first lens barrel, 326-second lens barrel, 327-short back intercept mirror part, 328-relay mirror, 3281-first pressure ring, 329-field lens, 3291-second pressure ring, 33-second height linear drive, 331-connecting plate, 34-first rotating motor;

[0069] 41-stage, 42-living organism to be tested, 43-three-axis moving mechanism, 431-third height linear drive, 432-two-axis moving assembly, 44-gas anesthesia module;

[0070] 5-light source assembly;

[0071] 61-first camera, 62-second camera, 63-first height linear drive, 631-lifting plate, 6311-long strip through hole, 6312-second slide rail, 64-moving plate, 651-first motor, 652-transmission belt;

[0072] 7- Controller. DETAILED DESCRIPTION

[0073] The technical solution of the utility model will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0074] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0075] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0076] In addition, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0077] like Figures 1 to 17 The figure shows a fully automatic living organism imaging device provided by the present embodiment, comprising a main box 1, wherein a mounting plate 11 is arranged at the upper end of the main box 1; a filter assembly 2 is arranged on the mounting plate 11, and a first light-shielding ring 21 is arranged at the upper end of the filter assembly 2; a lens assembly 3 is arranged just below the filter assembly 2 along the optical axis direction; a stage 41 is arranged below the lens assembly 3, and the stage 41 is used to place a living organism 42 to be tested; a light source assembly 5 is arranged above the stage 41, and the light source Component 5 is used to generate excitation light; a first Z-axis moving component is disposed at the upper end of the mounting plate 11, an X-axis moving component is disposed on the first Z-axis moving component, a first camera 61 and a second camera 62 are disposed on the X-axis moving component along the X direction, and the first camera 61 and the second camera 62 are respectively used to photograph the living organism 42 to be tested in different light bands and obtain in-situ images; when used in the experiment, one of the light inlet holes of the first camera 61 and the second camera 62 is selectively connected to the first light-shielding ring 21. It can be understood that the optical axis direction described in the text is parallel to the Z direction, and the X direction, Y direction, and Z direction described in the text refer to Figure 3 The X, Y and Z directions.

[0078] The fully automatic living organism imaging device of this embodiment drives the first camera 61 and the second camera 62 to move simultaneously along the X direction relative to the lens assembly 3 through the X-axis moving component. When it is necessary to shoot the living organism 42 to be tested on the stage 41 under different light bands, the first camera 61 or the second camera 62 with the corresponding band can be automatically switched to the position directly above the filter assembly 2 along the optical axis through the X-axis moving component, so that when used in the experiment, the light source assembly 5 generates excitation light of the corresponding wavelength to illuminate the living organism 42 to be tested on the stage 41, and the in-situ image under the corresponding light band can be accurately obtained; the entire camera switching and adjustment process does not require manual participation, has a high degree of automation, and is easy to operate. A first Z-axis moving assembly is also provided at the upper end of the mounting plate 11, and the X-axis moving assembly is provided on the first Z-axis moving assembly. In the process of moving and adjusting the positions of cameras of different wavelength bands, on the one hand, the light inlet of the camera can be driven to rise and be separated from the first light-shielding connecting ring 21 by the first Z-axis moving assembly, so as to avoid the first camera 61 and the second camera 62 from colliding with each other and interfering with the first light-shielding connecting ring 21 and causing damage during the movement of the first camera 61 and the second camera 62 along the X-direction driven by the X-axis moving assembly; on the other hand, the first camera 61 or the second camera 62 moved to the corresponding position along the X-direction can be driven to descend by the first Z-axis moving assembly until its light inlet is engaged with the first light-shielding connecting ring 21, so as to ensure sufficient light shielding of the optical path from the filter assembly 2 to the camera and ensure the quality of imaging; thereby, the light shielding effect of the optical channel formed between the camera, the filter assembly 2 and the lens assembly 3 can be ensured on the basis of quickly adjusting the corresponding switching of the cameras of different wavelength bands to the position just above the filter assembly 2 along the optical axis direction.

[0079] Specifically, the stage 41 is set as a constant temperature platform, and the anesthetized living organism 42 to be tested (such as a mouse, a rabbit, etc.) is placed on the constant temperature platform, which can effectively prevent the living organism 42 to be tested from dying from hypothermia after anesthesia. Fig.17 As shown, more specifically, the upper end surface of the stage 41 is provided with an air anesthesia module 44, which is used to continuously anesthetize the living organism 42 to be tested, so as to prevent the living organism 42 to be tested from waking up and moving during the experimental detection process, thereby affecting the test results.

[0080] Specifically, the light source assembly 5 includes a plurality of fluorescent light sources of different wavelengths, so that according to the requirements of actual experimental detection, several or more light sources of different wavelengths can be designed to respectively excite fluorescent probes of different colors.

[0081] like Figure 2 As shown, specifically, a light-proof inner liner 12 is provided in the main box body 1, and the light-proof inner liner 12 provides a cover for the filter assembly 2, the lens assembly 3, the first camera 61 and the second camera 62, effectively preventing external stray light from entering the imaging area at the upper end of the main box body 1, thereby improving the imaging quality.

[0082] Specifically, the first camera 61 is configured as a near-infrared camera, and the second camera 62 is configured as a visible light camera.

[0083] like Figure 3 , Figure 4 , Figure 6 , Figure 7 and Fig.11 As shown, in some embodiments of the utility model, a first through hole 111 is formed on the mounting plate 11 along the optical axis direction, and a second light-proof ring 22 is provided at the lower end of the filter assembly 2, the first light-proof ring 21 and the second light-proof ring 22 are arranged concentrically along the optical axis direction, and the projection along the optical axis direction falls within the range of the first through hole 111; the lens assembly 3 includes a second Z-axis moving assembly arranged on the mounting plate 11, a first rotating assembly is arranged on the second Z-axis moving assembly, a mounting disk 31 is arranged on the first rotating assembly, and the mounting disk 31 is driven by the first rotating assembly to rotate around the Z direction; the mounting disk 31 is arranged in the first through hole 111, and a plurality of lenses 32 with different magnifications are arranged on the mounting disk 31 at intervals along the circumference of the mounting disk 31; when used experimentally, one of the light exit holes of the plurality of lenses 32 is selected to be interlocked with the second light-proof ring 22. By installing lenses 32 of different magnifications on the mounting disk 31 clockwise or counterclockwise along the circumference of the mounting disk 31 in the order of magnification, and the mounting disk 31 is driven by the first rotating component to rotate around the Z direction, when it is necessary to use lenses 32 of different magnifications to photograph the living organism 42 to be tested on the stage 41, the mounting disk 31 can be driven by the first rotating component to rotate around the Z direction until the lens 32 of the corresponding magnification is switched to the position directly below the second light-shielding ring 22, so that the in-situ image under the lens 32 of the corresponding magnification can be accurately obtained. The entire switching and adjustment process of the lenses 32 of different magnifications does not require manual participation, has a high degree of automation, and the magnification switching positioning of the lens 32 is accurate. A second Z-axis moving component is also arranged on the mounting plate 11, and the first rotating component is arranged on the second Z-axis moving component. In the process of rotating and adjusting the position of the lens 32 of different magnifications, on the one hand, the light outlet of the lens 32 can be driven to descend and disengage from the second light-proof connecting ring 22, so as to avoid the interference and damage caused by the collision of the lens 32 of different magnifications with the second light-proof connecting ring 22 during the process of rotating around the Z direction driven by the first rotating component; on the other hand, after rotating and adjusting the lens 32 of the corresponding magnification to the set position, the lens 32 of the corresponding magnification can be driven by the second Z-axis moving component to rise to its light outlet and engage with the second light-proof connecting ring 22, so as to ensure that the optical path from the lens 32 to the filter assembly 2 is fully light-proof and the imaging quality is ensured; thereby, the light-proof effect of the optical channel formed between the camera, the filter assembly 2 and the lens 32 is ensured on the basis of quickly switching and adjusting the cameras of different bands and the lenses 32 of different magnifications to the set position.

[0084] like Figure 3 , Figure 4 and Figure 6 As shown, in some embodiments of the utility model, the second Z-axis moving assembly includes a second height linear driver 33 disposed on the mounting plate 11, the second height linear driver 33 is provided with a connecting plate 331 moving along the Z direction, and the connecting plate 331 is provided with the first rotating assembly along the Y direction toward the side wall of the X-axis moving assembly. The first rotating assembly, the mounting plate 31 and the lenses 32 of different magnifications can be moved up and down together with the connecting plate 331 under the drive of the second height linear driver 33, so as to ensure that when the mounting plate 31 is driven to rotate around the Z direction, the light outlet of the lens 32 is separated from the second light-shielding ring 22 without interference, and to ensure that the light outlet of the lens 32 of the corresponding magnification is buckled with the second light-shielding ring 22 when used in experimental tests, so as to ensure that the optical path from the lens 32 to the filter assembly 2 is fully light-shielded. At the same time, by arranging the first rotating assembly on the side of the connecting plate 331 facing the X-axis moving assembly, the rotation center of the mounting plate 31 is closer to the second light-shielding ring 22, so as to more stably drive the light exit hole of the lens 32 of the corresponding magnification to be buckled with the second light-shielding ring 22. Specifically, the second height linear drive 33 is configured as an electric cylinder or a pneumatic cylinder or a linear motor or a lead screw nut structure, and drives the first rotating assembly to reciprocate along the Z direction.

[0085] like Figure 6 As shown, in some embodiments of the utility model, the first rotating assembly includes a first rotating motor 34 arranged on the second Z-axis moving assembly along the Z direction, and the output end of the first rotating motor 34 is coaxially connected to the mounting plate 31. By controlling the rotation of the first rotating motor 34, the mounting plate 31 is driven to rotate around the Z direction, so as to realize the automatic switching and precise positioning of the lens 32 magnification. Specifically, the first rotating motor 34 is configured as a servo motor or a gear reduction motor. More specifically, the connecting plate 331 is folded downward along the Y direction toward the side wall of the X-axis moving assembly to form a vertical portion, and the lower end of the vertical portion is folded inward to form a horizontal portion arranged parallel to the Y direction. The first rotating motor 34 is arranged on the horizontal portion. On the one hand, the first rotating motor 34 is hidden and embedded in the space surrounded by the vertical portion and the horizontal portion, and is not easily damaged by external collision; on the other hand, the distance between the output end of the first rotating motor 34 and the mounting plate 31 along the Z direction is shortened, which is more convenient to smoothly drive the mounting plate 31 to rotate around the Z direction.

[0086] like Figure 6 and Fig.18As shown, in some embodiments of the utility model, at least one of the lenses 32 is configured as a long back focus lens, and mounting positions 311 are provided one by one on the mounting plate 31 corresponding to the positions of the long back focus lenses, and the mounting positions 311 penetrate the mounting plate 31 along the Z direction. The long back focus lenses include a coaxially connected fixing ring 321, a connecting ring 322 and a lens head 323, the fixing ring 321 is detachably connected to the mounting position 311, and one end along the Z direction is used to buckle with the second light-proof connecting ring 22, the connecting ring 322 is screwed on an internal thread at one end of the fixing ring 321 away from the second light-proof connecting ring 22 along the Z direction, and the lens head 323 is provided at one end of the connecting ring 322 away from the fixing ring 321 along the Z direction. Firstly, by detachably connecting the fixing ring 321 in the mounting position 311, and arranging the long back-intercept lens at the end of the filter assembly 2 away from the camera along the optical axis direction, there is sufficient space to conveniently remove the lens 32 of the original magnification and replace it with a lens 32 of another magnification according to the needs of actual experimental detection, and then rotate and adjust it to the corresponding position, so that the in-situ images under the lenses 32 of more magnifications can be accurately obtained, which is more practical. Secondly, considering that some interfaces have long back-focus lenses, such as the lens 32 of the interface types such as M52 and M42, the back focus reaches more than 40 mm, which is larger than the camera target surface, and the overall distance of the bottom surface of the rotating wheel 24 plus the thickness of the mounting plate 31, if such a long back-focus lens is directly used in the device of this embodiment, the lens 32 will be imaged behind the camera target surface, so that the camera cannot obtain a useful and clear image. In order to avoid the above technical problems, this embodiment screws the connecting ring 322 into the fixing ring 321. On the one hand, the overall length of the connecting ring 322 and the fixing ring 321 along the Z direction can be adjusted by rotating the thread, thereby eliminating the above-mentioned part of the back focus distance, ensuring that the camera obtains a useful and clear image; on the other hand, the rear imaging distance error caused by the processing of the various components of the long back-focus lens can be eliminated by rotating the thread, thereby ensuring the quality of imaging.

[0087] This embodiment does not limit the detachable connection structure between the fixing ring 321 and the mounting position 311. In order to improve the convenience of assembly and disassembly of the two on the basis of ensuring the stability of the connection between the two, preferably, the mounting position 311 is provided with an internal thread, and the outer wall of the fixing ring 321 is provided with a first external thread, and the first external thread matches the internal thread. Of course, in other embodiments, the fixing ring 321 and the mounting position 311 can also be connected by a detachable plug-in method of a slot and a plug post.

[0088] like Figure 6 , Fig.19 and Fig. 20As shown, at least one of the lenses 32 is configured as a short back focus lens, and the short back focus lens includes a coaxially connected fixing seat 324, a first lens barrel 325, a second lens barrel 326 and a short back focus lens portion 327, wherein the fixing seat 324 is detachably connected to the mounting plate 31, and one end along the Z direction is used to be buckled with the second light-shielding connecting ring 22; the fixing seat 324 has one end along the Z direction away from the second light-shielding connecting ring 22 and is internally screwed with the first lens barrel 325, and the first lens A relay lens 328 is connected to the barrel 325 through a first pressing ring 3281, and the first pressing ring 3281 is located at one end of the relay lens 328 away from the fixing seat 324; the second barrel 326 is provided at one end of the first barrel 325 away from the fixing seat 324 along the Z direction, and a field lens 329 is connected to the second barrel 326 through a second pressing ring 3291; and the short back focus lens portion 327 is provided at one end of the second barrel 326 away from the first barrel 325 along the Z direction. Firstly, by detachably connecting the fixing seat 324 to the mounting plate 31, and the short back focus lens is arranged at one end of the filter assembly 2 away from the camera along the optical axis direction, there is sufficient space to conveniently remove the original magnification lens 32 and replace it with another magnification lens 32 according to the needs of actual experimental detection, and then rotate and adjust to the corresponding position, so that the in-situ images under the lenses 32 of more magnifications can be accurately obtained, which is more practical. Secondly, considering that the mounting plate 11 and the filter assembly 2 are inserted between the short back focus lens and the camera, many short back focus lenses that were originally directly connected to the camera are directly mounted on the mounting plate 31. Due to the small back focus of the short back focus lens, it is impossible to form an image on the camera target surface, so that the camera cannot obtain a useful and clear image. In order to avoid the above technical problems, this embodiment sequentially arranges a field lens 329 and a relay lens 328 in the short back focus lens portion 327 along the optical axis direction toward the second light-shielding ring 22, as shown in FIG. Fig. 20 The short back intercept relay imaging principle diagram is shown in the figure, where the original object to be measured is at Fig. 20 At the plane position indicated by B, the back focus of the short back focus mirror portion 327 is Fig. 20 The L1 shown in the figure is imaged at Fig. 20 At the plane indicated by C in the middle, a field lens 329 is first placed at the plane of the imaging position. The field lens 329 converges the imaging beam through the relay lens 328, and the back intercept is increased by the relay lens 328. The increased back intercept is the relay back intercept L2, and the final imaging target surface is Fig. 20The plane position indicated by D in the figure; the short back focus lens can be lengthened relative to the original short back focus lens part 327 after the relay, thereby eliminating the back focus length increased by the mounting plate 11 and the filter assembly 2, and realizing the rapid switching and adjustment of cameras of different bands and lenses 32 of different magnifications to the set position without affecting the imaging effect. Furthermore, by screwing the first lens barrel 325 into the fixing seat 324, the overall length of the first lens barrel 325 and the fixing seat 324 along the Z direction can be adjusted by rotating the thread, eliminating the rear imaging distance error generated during the processing of the various components of the short back focus lens, and ensuring the quality of imaging.

[0089] It should be noted that, according to the design structure, the mounting plate 31 rises to the top working position (the position where the light outlet hole of the lens 32 of the corresponding magnification is buckled with the second light-shielding ring 22), and the distance from the camera target surface to the mounting bottom surface of the mounting plate 31 is the adapted back focus in this embodiment. The lens 32 with a back focus less than this distance (adapted back focus) is a short back focus lens, and the lens 32 with a back focus greater than this distance is a long back focus lens.

[0090] Specifically, this embodiment does not restrict the detachable connection structure between the fixing seat 324 and the mounting plate 31. In order to improve the convenience of assembly and disassembly of the two while ensuring the stability of the connection between the two, preferably, the position on the mounting plate 31 corresponding to the fixing seat 324 is penetrated along the Z direction to form an internal threaded hole, and the outer wall of the fixing seat 324 is provided with a second external thread, and the second external thread matches the internal threaded hole. Of course, in other embodiments, the fixing seat 324 and the mounting plate 31 can also be connected by a detachable plug-in method of a slot and a plug post.

[0091] like Figures 3 to 5 as well as Fig.12As shown, in some embodiments of the utility model, both sides of the mounting plate 11 along the X direction are provided with first slide rails 112 along the Z direction, the first Z-axis moving component includes a first height linear driver 63 arranged on the mounting plate 11, the first height linear driver 63 is provided with a lifting plate 631 moving along the Z direction, the lifting plate 631 is arranged above the mounting plate 11 along the X direction, and is slidably connected to the first slide rail 112 along the Z direction on both sides along the X direction; the lifting plate 631 is formed with a long strip through hole 6311 along the Z direction corresponding to the position of the first light-shielding ring 21, the long strip through hole 6311 is arranged along the X direction, and is used for the first light-shielding ring 21 to be embedded and interlocked with the light inlet hole of the first camera 61 or the second camera 62; the upper end of the lifting plate 631 is provided with There are two second slide rails 6312, which are relatively arranged on both sides of the long strip through hole 6311 along the Y direction, and a moving plate 64 is slidably connected to the two second slide rails 6312 along the X direction. The first camera 61 and the second camera 62 are arranged on the moving plate 64 at intervals along the X direction, and the light holes of the first camera 61 and the second camera 62 pass through the moving plate 64 and extend into the long strip through hole 6311; the X-axis moving component includes a main pulley and a slave pulley, and the main pulley is driven to rotate by the first motor 651. The main pulley and the slave pulley are respectively arranged on both sides of the lifting plate 631 along the X direction, and the main pulley and the slave pulley are connected by a transmission belt 652, and the moving plate 64 is connected to the transmission belt 652. First, under the guidance of the first slide rail 112, the X-axis moving assembly, the first camera 61 and the second camera 62 are driven by the first height linear driver 63 to smoothly move up and down with the lifting plate 631, thereby ensuring that when the first camera 61 and the second camera 62 are driven to move along the X direction, the light inlet holes of the first camera 61 and the second camera 62 are separated from the first light-shielding ring 21 without interference, and ensuring that the light inlet holes of the first camera 61 or the second camera 62 are buckled with the first light-shielding ring 21 when used in experimental tests, thereby ensuring that the optical path from the filter assembly 2 to the camera is fully shielded from light. Secondly, by providing a long strip through hole 6311 on the lifting plate 631, the long strip through hole 6311 can provide an escape space for the first light-shielding ring 21 to move up and down, and can also provide an escape space for the light inlet of the first camera 61 and the second camera 62 to move along the X direction, ensuring that the light inlet of the camera of the corresponding band can be accurately connected with the first light-shielding ring 21 while the cameras of different bands are quickly moved to the corresponding positions.Furthermore, under the guidance of the second slide rail 6312, the first camera 61 and the second camera 62 are smoothly moved along the X direction with the moving plate 64 during the clockwise or counterclockwise rotation of the transmission belt 652, so as to automatically and accurately switch the first camera 61 or the second camera 62 with the corresponding band to the position directly above the lens assembly 3 along the optical axis. Specifically, the first height linear driver 63 is configured as an electric cylinder or an air cylinder or a linear motor or a lead screw nut structure, and drives the lifting plate 631 to reciprocate along the Z direction. More specifically, the X-axis moving assembly is configured as an electric cylinder or an air cylinder or a linear motor or a lead screw nut structure, and drives the moving plate 64 to reciprocate along the X direction.

[0092] like Figure 3 , Figure 4 , Figure 7 , Figure 8 and Fig.11 As shown, in some embodiments of the utility model, the filter assembly 2 includes a switching bin 23 arranged at the upper end of the mounting plate 11, and the switching bin 23 and the second Z-axis moving assembly are respectively arranged on both sides of the X-axis moving assembly along the Y direction; a mounting cavity 231 is arranged in the switching bin 23; the first light-proof connecting ring 21 and the second light-proof connecting ring 22 are respectively arranged on the upper end surface and the lower end surface of the switching bin 23, and are connected to the mounting cavity 231; a rotating wheel 24 is arranged in the mounting cavity 231, and the rotating wheel 24 is driven by the second rotating assembly to rotate around the Z direction, and a plurality of filters 25 are arranged on the rotating wheel 24 at circumferential intervals along the rotating wheel 24, and the wavelength bands of the filters 25 located at different circumferential positions are different; when used in experiments, one of the plurality of filters 25 is selected to be concentrically arranged with the first light-proof connecting ring 21 and the second light-proof connecting ring 22 along the optical axis direction. First, by respectively arranging the switching bin 23 and the second Z-axis moving assembly on both sides of the X-axis moving assembly along the Y direction, the sizes of the filter assembly 2, the lens assembly 3 and the camera along the Z direction are reduced, thereby further simplifying and reducing the structure of the device of this embodiment; secondly, by arranging filters 25 of different bands on the rotating wheel 24 along the circumference of the rotating wheel 24 in the order of bands, according to the needs of actual experimental detection, under the action of the second rotating assembly, the positions of the filters 25 of different bands can be rotated synchronously with the rotation of the rotating wheel 24 until the filters 25 of the corresponding band are rotated and switched to a position that is concentrically arranged with the first light-shielding ring 21 and the second light-shielding ring 22 along the optical axis direction, thereby achieving the effect of quickly switching filters 25 of different bands for corresponding shooting, which is conducive to improving the flexibility of use.

[0093] like Figures 7 to 10 as well as Figures 13 to 16As shown, in some embodiments of the utility model, a mounting hole 241 is provided on the rotating wheel 24 at a position corresponding to the filter 25, and the mounting hole 241 penetrates the rotating wheel 24 along the radially outward end of the rotating wheel 24 to form a plug-in slot 2411, and a retaining spring 26 is provided on the radially inward end of the rotating wheel 24. The filter 25 includes a connecting member 251 and a lens 252. A connecting hole is formed through the connecting member 251 along the Z direction, and the lens 252 is arranged in the connecting hole. The connecting member 251 is slidably inserted in the plug-in slot 2411 and is detachably locked by the retaining spring 26; the front end surface of the switching chamber 23 along the X direction is penetrated along the X direction at least corresponding to a position of the plug-in slot 2411 to form a plug-in hole 232, and a connecting cover 27 is detachably connected to the plug-in hole 232. Considering that the filter assembly 2 is arranged between the lens assembly 3 and the camera along the optical axis direction, if it is necessary to remove the original filter 25 on the rotating wheel 24 and replace it with a filter 25 of another band in order to meet more experimental detection requirements, it is often necessary to remove the camera at the top or remove the lens assembly 3 at the bottom. However, it is difficult to disassemble and replace the filter 25 from the top or from the bottom, and the workload is large. In order to solve the above problems, the device of this embodiment first forms a plug hole 232 through the front end surface of the switching chamber 23 along the X direction at a position corresponding to at least one plug slot 2411, and A connection cover 27 is detachably connected to the socket 232. When it is necessary to remove the original filter 25 on the rotating wheel 24 and replace it with a filter 25 of another band, first rotate and adjust the filter 25 to be removed to the position corresponding to the socket 232, and then remove the connection cover 27 from the front end surface of the switching chamber 23, remove the original filter 25 and replace it with a filter 25 of another band, thereby realizing the convenient removal of the original filter 25 on the rotating wheel 24 and replacing it with a filter 25 of another band without removing the camera at the top or the lens assembly 3 at the bottom, and the operation is simple. Secondly, a retaining spring 26 is provided at one end of the mounting hole 241 facing radially inwardly of the rotating wheel 24. When the optical filter 25 needs to be installed in the corresponding mounting hole 241, after the connecting piece 251 is inserted radially inwardly of the rotating wheel 24 to the set position in the plug-in slot 2411, the connecting piece 251 is pressed by the spring force formed by the deformation of the retaining spring 26, so that the optical filter 25 can be locked. The pressing force applied by the retaining spring 26 on the connecting piece 251 can effectively prevent the optical filter 25 from moving radially relative to the corresponding mounting hole 241 during the synchronous rotation of the rotating wheel 24. When the optical filter 25 needs to be removed from the corresponding mounting hole 241, only a pulling force greater than the pressing force applied by the retaining spring 26 on the connecting piece 251 is required to pull the optical filter 25 out radially of the rotating wheel 24. The entire disassembly and assembly process is convenient and labor-saving.It can be understood that, radially inward of the rotating wheel 24 mentioned in the text refers to radially toward the center of the rotating wheel 24 ; radially outward of the rotating wheel 24 mentioned in the text refers to radially away from the center of the rotating wheel 24 .

[0094] It should be noted that if Fig.16 and Fig.14 As shown, one end of the connecting member 251 facing the retaining spring 26 is configured as an arc portion 2512 . When the retaining spring 26 is in an initial state, the radius of the arc segment of the retaining spring 26 facing the arc portion 2512 is smaller than the radius of the arc portion 2512 .

[0095] like Fig.14 and Fig.15 As shown, specifically, a third through hole 261 is formed on the retaining ring 26 along the radial direction of the rotating wheel 24, and a second threaded hole 2414 is provided on the surrounding wall of the mounting hole 241 at a position corresponding to the third through hole 261; when the retaining ring 26 is assembled in the mounting hole 241, the locking bolt passes through the third through hole 261 and is screwed into the second threaded hole 2414, thereby realizing a detachable connection between the retaining ring 26 and the mounting hole 241.

[0096] In order to facilitate the removal of the connection cover 27 from the jack 232, the original filter 25 on the rotating wheel 24 can be removed and replaced with a filter 25 of another wavelength band; Figure 1 As shown, specifically, a switching door 13 is provided on the front end surface of the main box body 1 along the X direction at a position corresponding to the connection cover 27 and can be opened and closed.

[0097] like Fig.15 As shown, specifically, the mounting hole 241 includes a light-transmitting portion 2415 and a mounting portion 2416, the mounting portion 2416 is located on the upper end surface of the rotating wheel 24, and the mounting portion 2416 passes through the rotating wheel 24 along the radially outward end of the rotating wheel 24 to form the plug-in slot 2411, and the light-transmitting portion 2415 is connected and arranged at the lower end of the mounting portion 2416, and passes through the rotating wheel 24 along the optical axis direction; when used experimentally, one of the multiple light-transmitting portions 2415 is selected to be concentrically arranged with the first light-shielding ring 21 and the second light-shielding ring 22 along the optical axis direction.

[0098] Specifically, the filters 25 of different wavelength bands are installed in the corresponding installation holes 241 clockwise or counterclockwise according to the order of the wavelength bands, and the filters 25 of different wavelength bands can be switched by rotating the wheel 24.

[0099] like Figure 4 , Figure 7 , Figure 8 , Fig. 9 and Fig.16As shown, in some embodiments of the present invention, each of the connecting parts 251 is provided with an RFID tag 253, and a RFID reading and writing module 254 is provided at a position corresponding to one of the RFID tags 253 on the switching bin 23, and the RFID reading and writing module 254 is electrically connected to the controller 7, and the controller 7 is electrically connected to the second rotating component for rotation; the first Z-axis moving component, the X-axis moving component, the second Z-axis moving component and the first rotating component are all electrically connected to the controller 7. By pre-storing the band of the corresponding filter 25 and the hole number information of the mounting hole 241 where the corresponding filter 25 is located in the RFID tag 253, on the one hand, in the process of driving the filters 25 of different bands to rotate synchronously with the rotating wheel 24 through the second rotating component according to the needs of actual experimental detection, the band information of the corresponding filter 25 pre-stored in the RFID tag 253 located directly below the RFID reading and writing module 254 is read, and the information is transmitted to the controller 7. The controller 7 determines whether the filter 25 of the required band is rotated and switched to a position concentrically arranged along the optical axis with the first light-shielding ring 21 and the second light-shielding ring 22 according to the information transmitted by the RFID reading and writing module 254, and controls the second rotating component to continue to rotate according to the judgment result, to ensure that the filter 25 of the corresponding band is switched quickly and accurately for corresponding shooting. On the other hand, when it is necessary to remove the original filter 25 on the rotating wheel 24 and replace it with a filter 25 of another wavelength band, the hole number information of the mounting hole 241 where the corresponding filter 25 is located, which is pre-stored in the RFID tag 253 located directly below the RFID read / write module 254, is read and transmitted to the controller 7. The controller 7 determines whether the filter 25 to be removed is rotated and switched to the position of the corresponding jack 232 according to the information transmitted by the RFID read / write module 254, and controls whether the second rotating component continues to rotate according to the judgment result, so as to ensure that the original filter 25 on the rotating wheel 24 is accurately removed and replaced with a filter 25 of another wavelength band. Specifically, the controller 7 is set as an STM32 control motherboard.

[0100] Specifically, the above-mentioned controller 7 can be a PLC single-chip microcomputer or other computer equipment. The controller 7 can control the opening and closing, moving direction and amount of the first Z-axis moving component, can control the opening and closing, moving direction and amount of the X-axis moving component, can control the opening and closing, moving direction and amount of the second Z-axis moving component, can control whether the first rotating component rotates, the rotation angle and the rotation direction, and can control whether the second rotating component rotates, the rotation angle and the rotation direction.

[0101] like Fig.13As shown, in some embodiments of the present invention, a hole number label 2413 is provided in the plug-in slot 2411. In the process of removing the original filter 25 on the rotating wheel 24 and replacing it with a filter 25 of another band, the operator can visually judge whether the filter 25 in the installation hole 241 corresponding to the plug-in hole 232 is the filter 25 to be removed by observing the information of the hole number label 2413, thereby further ensuring the accuracy of replacing the filter 25.

[0102] like Figure 7 and Figure 8 As shown, in some embodiments of the utility model, positioning members 281 are provided one by one at the positions of the rotating wheel 24 corresponding to the filter 25, and a positioning sensor 282 is provided at the position corresponding to one of the positioning members 281 in the mounting cavity 231, and the positioning sensor 282 is electrically connected to the controller 7. When the positioning members 281 at different circumferential positions pass directly under the positioning sensor 282, the positioning sensor 282 will generate different signals and transmit them to the controller 7. The controller 7 can determine which band of filter 25 is located at a position concentrically arranged along the optical axis with the first light-shielding ring 21 and the second light-shielding ring 22 through the type of signal transmitted by the positioning sensor 282, and control whether the second rotating component continues to rotate based on the judgment result, thereby ensuring that the filter 25 of the corresponding band is switched quickly and accurately for corresponding shooting; it can also determine which band of filter 25 is located at a position aligned with the socket 232, and control whether the second rotating component continues to rotate based on the judgment result, thereby ensuring that even if the RFID read-write module 254 fails, the original filter 25 on the rotating wheel 24 can be accurately removed and replaced with a filter 25 of other bands.

[0103] like Fig.13 , Fig.15 and Fig.16 As shown, in some embodiments of the present invention, the two side walls of the plug slot 2411 are concavely provided with slide grooves 2412, and the two side walls of the connector 251 are respectively provided with slide portions 2511 at positions corresponding to the slide grooves 2412. Through the mutual guiding effect of the slide grooves 2412 and the slide portions 2511, the connector 251 is accurately assembled in the corresponding mounting hole 241, thereby ensuring the quality of imaging.

[0104] like Fig.10As shown, in some embodiments of the utility model, a first magnet 233 is provided on the end surface of the switching chamber 23 facing the connecting cover 27, and a second magnet is provided on the connecting cover 27 at a position corresponding to the first magnet 233. The second magnet and the first magnet 233 have opposite magnetic properties, and the connecting cover 27 is stably and tightly embedded in the socket 232 through the magnetic attraction force generated between the first magnet 233 and the second magnet with opposite magnetic properties, thereby ensuring the light-shielding effect of the mounting cavity 231 and the imaging quality.

[0105] like Figure 7 , Figure 8 , Fig. 9 and Fig.11 As shown, in some embodiments of the present invention, the upper end of the switching chamber 23 is formed with a through hole along the Z direction, and the second rotating assembly includes a second rotating motor 29 arranged on the upper end surface of the switching chamber 23 along the Z direction, and the output end of the second rotating motor 29 passes through the through hole and is coaxially connected to the rotating wheel 24. The optical filters 25 of different bands can be synchronously rotated and adjusted with the rotating wheel 24 around the Z direction under the drive of the second rotating motor 29, so as to realize the automatic switching and precise positioning of the optical filters 25 of different bands. Specifically, the second rotating motor 29 is set as a servo motor or a gear reduction motor.

[0106] like Figures 7 to 9 As shown, in some embodiments of the utility model, the switching chamber 23 includes a first part 234 and a second part 235 which are separately arranged, the first part 234 is arranged at the upper end of the second part 235, and the upper end surface of the second part 235 is provided with a plurality of first threaded holes 2351 at intervals along the cross-sectional profile of the second part 235 perpendicular to the Z direction, and a second through hole 2341 is provided at a position corresponding to the first threaded hole 2351 on the first part 234, and the second through hole 2341 penetrates the first part 234 along the Z direction; when it is necessary to assemble the first part 234 and the second part 235 to form the switching chamber 23, it is only necessary to use a fastening bolt to pass through the second through hole 2341 and screw it into the corresponding first threaded hole 2351, and the end surfaces of the first part 234 and the second part 235 facing each other form a mounting cavity 231 to complete the installation; when it is necessary to disassemble the switching chamber 23 to repair the internal parts of the switching chamber 23, it is only necessary to screw down the fastening bolt from the corresponding first threaded hole 2351; the entire disassembly and assembly process is convenient to operate.

[0107] like Figure 1 , Figure 2 and Fig.17As shown, in some embodiments of the present invention, a three-axis moving mechanism 43 is provided at the lower inner end of the main box 1, and the stage 41 is provided on the three-axis moving mechanism 43, and is driven by the three-axis moving mechanism 43 to move in the X, Y and Z directions. The three-axis moving mechanism 43 is used to drive the living organism 42 to be tested placed on the stage 41 to move in three dimensions along the X, Y and Z directions, so as to ensure the spacing accuracy between the living organism 42 to be tested and the light inlet of the lens 32 along the Z direction while ensuring that the living organism 42 to be tested is located at the center of the imaging field of view, thereby ensuring the quality of imaging.

[0108] like Figure 1 and Figure 2 As shown, a supporting bottom plate 15 is provided at the lower end of the interior of the main box body 1, and the gap between the supporting bottom plate 15 and the inner wall of the main box body 1 is smaller than the size of the living organism 42 to be tested, and the loading platform 41 is provided on the upper end surface of the supporting bottom plate 15, which effectively prevents the living organism 42 to be tested from falling into the lower space of the main box body 1 through the gap between the side wall of the supporting bottom plate 15 and the inner wall of the main box body 1 due to incomplete anesthesia.

[0109] like Figure 1 and Figure 2 As shown, specifically, the mounting plate 11 and the supporting bottom plate 15 sequentially separate the interior of the main box 1 into an imaging chamber 16, an operating chamber 17 and a motion control chamber 18 from top to bottom along the Z direction. By sequentially separating the interior of the light-proof inner container 12 into the imaging chamber 16, the operating chamber 17 and the motion control chamber 18 from top to bottom, the components respectively located in the imaging chamber 16, the operating chamber 17 and the motion control chamber 18 are independent of each other to avoid mutual influence, thereby ensuring the quality of imaging.

[0110] like Figure 1 , Figure 2 and Fig.17 As shown, more specifically, the three-axis moving mechanism 43 includes a third height linear driver 431 and a two-axis moving assembly 432. The third height linear driver 431 is arranged in the motion control bin 18 along the Z direction. The telescopic end of the third height linear driver 431 extends through the supporting base plate 15 to the operating bin 17 and is connected to the two-axis moving assembly 432. The worktable 41 is arranged at the moving end of the two-axis moving assembly 432.

[0111] Specifically, the third height linear driver 431 is configured as an electric cylinder or a pneumatic cylinder or a linear motor, and drives the two-axis moving assembly 432 and the stage 41 to reciprocate along the Z direction.

[0112] Specifically, the two-axis moving component 432 is a conventional two-axis moving structure, which can realize the function of two-dimensional moving driving along the X direction and the Y direction.

[0113] like Figure 1 As shown, specifically, the front end surface of the main box body 1 along the X direction is opened and closed at a position corresponding to the sample stage 41; during the experiment, the light-proof box door 14 is opened to place the anesthetized living organism 42 to be tested on the sample stage 41.

[0114] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the invention of the utility model.

Claims

1. A fully automatic in vivo biological imaging device, characterized in that: include: A main box body (1) is provided with a mounting plate (11) at the upper end thereof; A filter assembly (2) is arranged on the mounting plate (11), and a first light-shielding ring (21) is arranged at the upper end of the filter assembly (2); A lens assembly (3) is arranged directly below the optical filter assembly (2) along the optical axis; A stage (41) is arranged below the lens assembly (3) and is used to place a living organism (42) to be tested; A light source assembly (5) is arranged above the object stage (41) and is used to generate excitation light; A first Z-axis moving assembly is arranged on the upper end of the mounting plate (11); An X-axis moving component is arranged on the first Z-axis moving component; a first camera (61) and a second camera (62) are arranged on the X-axis moving component at intervals along the X direction, and the first camera (61) and the second camera (62) are respectively used to photograph the living organism (42) to be tested under different light bands and obtain in-situ images; When used in an experiment, one of the light inlet holes of the first camera (61) and the second camera (62) is locked with the first light-shielding ring (21).

2. A fully automatic living organism imaging device according to claim 1, characterized in that: The mounting plate (11) is provided with a first through hole (111) penetrating along the optical axis direction; a second light-shielding ring (22) is provided at the lower end of the filter assembly (2); the first light-shielding ring (21) and the second light-shielding ring (22) are arranged concentrically along the optical axis direction, and their projections along the optical axis direction fall within the range of the first through hole (111); the lens assembly (3) comprises: A second Z-axis moving assembly is arranged on the mounting plate (11); A first rotating assembly, disposed on the second Z-axis moving assembly; A mounting plate (31) is arranged on the first rotating assembly and driven by the first rotating assembly to rotate around the Z direction; the mounting plate (31) is arranged in the first through hole (111), and a plurality of lenses (32) with different magnifications are arranged on the mounting plate (31) at intervals along the circumference of the mounting plate (31); During experimental use, one of the light exit holes of the plurality of lenses (32) is selectively connected to the second light-shielding connecting ring (22).

3. A fully automatic living organism imaging device according to claim 2, characterized in that: The optical filter assembly (2) comprises: A switching bin (23) is arranged at the upper end of the mounting plate (11); the switching bin (23) and the second Z-axis moving assembly are respectively arranged on both sides of the X-axis moving assembly along the Y direction; a mounting cavity (231) is arranged in the switching bin (23); the first light-shielding connecting ring (21) and the second light-shielding connecting ring (22) are respectively arranged on the upper end surface and the lower end surface of the switching bin (23), and are in communication with the mounting cavity (231); A rotating wheel (24) is arranged in the installation cavity (231) and driven by a second rotating assembly to rotate around the Z direction, a plurality of filters (25) are arranged on the rotating wheel (24) at intervals along the circumference of the rotating wheel (24), and the filters (25) located at different circumferential positions have different wavelength bands; When used in an experiment, one of the plurality of filters (25) is arranged concentrically with the first light-shielding connecting ring (21) and the second light-shielding connecting ring (22) along the optical axis direction.

4. The fully automatic living organism imaging device according to claim 3, characterized in that: A mounting hole (241) is provided at a position on the rotating wheel (24) corresponding to the optical filter (25); an end of the mounting hole (241) facing radially outwards of the rotating wheel (24) penetrates the rotating wheel (24) to form a plug-in slot (2411); a retaining spring (26) is provided at an end of the mounting hole (241) facing radially inwards of the rotating wheel (24); the optical filter (25) comprises a connecting piece (251) and a lens (252); the connecting piece A connecting hole is formed along the Z direction on the upper surface (251), the lens (252) is arranged in the connecting hole, the connecting member (251) is slidably inserted in the insertion groove (2411), and is detachably locked by the retaining spring (26); the front end surface of the switching chamber (23) along the X direction corresponds to at least one position of the insertion groove (2411) to form an insertion hole (232) along the X direction, and a connecting cover (27) is detachably connected to the insertion hole (232).

5. A fully automatic living organism imaging device according to claim 4, characterized in that: Each of the connecting members (251) is provided with an RFID tag (253); a position on the switching bin (23) corresponding to one of the RFID tags (253) is provided with an RFID reading / writing module (254); the RFID reading / writing module (254) is electrically connected to a controller (7); the controller (7) is electrically connected to the second rotating assembly for rotation; the first Z-axis moving assembly, the X-axis moving assembly, the second Z-axis moving assembly and the first rotating assembly are all electrically connected to the controller (7); And / or, the two side walls of the plug-in slot (2411) are recessed with a slide groove (2412), and the two side walls of the connecting member (251) are respectively provided with a slide portion (2511) at a position corresponding to the slide groove (2412); And / or, a first magnet (233) is provided on the end surface of the switching chamber (23) facing the connecting cover (27), and a second magnet is provided on the connecting cover (27) at a position corresponding to the first magnet (233), and the second magnet has opposite magnetic properties to the first magnet (233); And / or, a hole position number label (2413) is provided in the plug-in slot (2411).

6. The fully automatic living organism imaging device according to claim 5, characterized in that: Positioning members (281) are provided one by one on the rotating wheel (24) at positions corresponding to the optical filters (25); a positioning sensor (282) is provided in the mounting cavity (231) at a position corresponding to one of the positioning members (281); and the positioning sensor (282) is electrically connected to the controller (7).

7. A fully automatic living organism imaging device according to claim 3 or 4, characterized in that: The upper end of the switching chamber (23) is penetrated by a through hole along the Z direction, the second rotating assembly comprises a second rotating motor (29) arranged on the upper end surface of the switching chamber (23) along the Z direction, and the output end of the second rotating motor (29) passes through the through hole and is coaxially connected to the rotating wheel (24); And / or, the switching chamber (23) comprises a first part (234) and a second part (235) which are separately arranged, the first part (234) being arranged at the upper end of the second part (235), a plurality of first threaded holes (2351) being arranged at intervals on the upper end surface of the second part (235) along the cross-sectional contour of the second part (235) perpendicular to the Z direction, a second through hole (2341) being arranged on the first part (234) at a position corresponding to the first threaded hole (2351), the second through hole (2341) penetrating the first part (234) along the Z direction, when the first part (234) and the second part (235) are assembled to form the switching chamber (23), a fastening bolt passes through the second through hole (2341) and is screwed into the corresponding first threaded hole (2351), and the end surfaces of the first part (234) and the second part (235) facing each other form the mounting cavity (231).

8. A fully automatic living organism imaging device according to any one of claims 2 to 6, characterized in that: The second Z-axis moving assembly comprises a second height linear drive (33) arranged on the mounting plate (11), the second height linear drive (33) being provided with a connecting plate (331) moving along the Z direction, and the connecting plate (331) being provided with the first rotating assembly on a side wall along the Y direction toward the X-axis moving assembly; And / or, the first rotating assembly comprises a first rotating motor (34) arranged on the second Z-axis moving assembly along the Z direction, and an output end of the first rotating motor (34) is coaxially connected to the mounting plate (31); And / or, at least one of the lenses (32) is configured as a long back-focus lens, a mounting position (311) is provided on the mounting plate (31) at a position corresponding to the long back-focus lens, the mounting position (311) penetrates the mounting plate (31) along the Z direction, the long back-focus lens comprises a coaxially connected fixing ring (321), a connecting ring (322) and a lens portion (323), the fixing ring (321) is detachably connected in the mounting position (311), and one end along the Z direction is used for buckling with the second light-shielding connecting ring (22), one end of the fixing ring (321) along the Z direction away from the second light-shielding connecting ring (22) is internally threadedly screwed with the connecting ring (322), and the lens portion (323) is provided at one end of the connecting ring (322) along the Z direction away from the fixing ring (321); And / or, at least one of the lenses (32) is configured as a short back focus lens, the short back focus lens comprising a coaxially connected fixing seat (324), a first lens barrel (325), a second lens barrel (326) and a short back focus lens portion (327), the fixing seat (324) being detachably connected to the mounting plate (31), and one end along the Z direction being used for buckling with the second light-shielding connecting ring (22); one end of the fixing seat (324) along the Z direction away from the second light-shielding connecting ring (22) is internally threadedly screwed with the first lens barrel (325), and the first lens barrel (3 25) is connected with a relay lens (328) via a first pressing ring (3281), and the first pressing ring (3281) is located at an end of the relay lens (328) away from the fixing seat (324); the second lens barrel (326) is provided at an end of the first lens barrel (325) away from the fixing seat (324) along the Z direction, and a field lens (329) is connected to the second lens barrel (326) via a second pressing ring (3291); and the short back focus mirror portion (327) is provided at an end of the second lens barrel (326) away from the first lens barrel (325) along the Z direction.

9. A fully automatic living organism imaging device according to any one of claims 1 to 6, characterized in that: The mounting plate (11) is provided with first slide rails (112) along the Z direction on both sides along the X direction. The first Z-axis moving component comprises a first height linear driver (63) arranged on the mounting plate (11). The first height linear driver (63) is provided with a lifting plate (631) moving along the Z direction. The lifting plate (631) is arranged above the mounting plate (11) along the X direction and is slidably connected to the first slide rails (112) along the Z direction on both sides along the X direction. The lifting plate (631) is formed with a long strip through hole (6311) penetrating along the Z direction at a position corresponding to the first light-shielding ring (21). The long strip through hole (6311) is arranged along the X direction and is used for the first light-shielding ring (21) to be embedded and interlocked with the light inlet of the first camera (61) or the second camera (62); two second slide rails (6312) are arranged at the upper end of the lifting plate (631), and a movable plate (64) is slidably connected along the X direction on the two second slide rails (6312), and the first camera (61) and the second camera (62) are arranged on the movable plate (64) at intervals along the X direction, and the light inlet of the first camera (61) and the second camera (62) passes through the movable plate (64) and extends into the long strip through hole (6311); The X-axis moving component includes a main pulley and a slave pulley, the main pulley is driven to rotate by a first motor (651), the main pulley and the slave pulley are respectively arranged on both sides of the lifting plate (631) along the X direction, a transmission belt (652) is sleeved between the main pulley and the slave pulley, and the moving plate (64) is connected to the transmission belt (652).

10. The fully automatic living organism imaging device according to claim 1, characterized in that: A three-axis moving mechanism (43) is provided at the lower end of the interior of the main box (1); the loading platform (41) is arranged on the three-axis moving mechanism (43) and is driven by the three-axis moving mechanism (43) to move along the X direction, the Y direction and the Z direction; And / or, a light-proof inner container (12) is provided in the main box (1); And / or, a supporting base plate (15) is provided at the lower end of the interior of the main box (1), the gap between the supporting base plate (15) and the inner wall of the main box (1) is smaller than the size of the living organism (42) to be tested, and the stage (41) is provided on the upper end surface of the supporting base plate (15); the mounting plate (11) and the supporting base plate (15) separate the interior of the main box (1) into an imaging chamber (16), an operating chamber (17) and a motion control chamber (18) in sequence from top to bottom along the Z direction.

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