Experiment teaching aid for comparing breathing modes of saccharomycetes cells
By adding nutrient additives and stirring devices to the experimental teaching aids for yeast cell respiration mode comparison, the problem of insufficient mixing of yeast and nutrients was solved, significantly speeding up the reaction speed and reducing the experiment time.
Patent Information
- Application Number
- CN202421752315.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-23
- Publication Date
- 2025-05-27
- Estimated Expiration
- 2034-07-23
AI Technical Summary
Comparison of yeast cell breathing methods in teaching experiments. Due to insufficient mixing of yeast and nutrients, the reaction speed is slow, which increases the experimental time.
An experimental teaching aid was designed, including a display board, aerobic and anaerobic reaction bottle, a nutrient additive and a stirring device. Inject nutrients into the reaction bottle through a nutrient additive, and stir the mixture using a magnetic stirrer to ensure that the yeast and nutrients are fully mixed.
Through sufficient mixing and contact, the aerobic and anaerobic respiration reaction speed of yeasts is accelerated, significantly reducing the experimental time.
Smart Images
Figure CN222907908U_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the technical field of experimental teaching aids, and in particular to an experimental teaching aid for comparing the respiration modes of yeast cells. Background Art
[0002] The experiment to explore the breathing mode of yeast cells is the first comparative experiment in high school biology. It aims to compare the types and products of respiration of yeast under aerobic and anaerobic conditions. The products of yeast respiration under aerobic conditions are carbon dioxide and water, and the products of yeast respiration under anaerobic conditions are carbon dioxide and alcohol.
[0003] However, the experiments in teaching often have the following problems: the mixture of yeast and nutrients is in a static state, and the yeast and nutrients cannot be fully mixed and contacted, resulting in a slow experimental reaction speed and increasing the time of the entire experiment, so further improvement is needed. Utility Model Content
[0004] In order to reduce the experimental time, the present application provides an experimental teaching aid for comparing the respiration modes of yeast cells.
[0005] The present application provides an experimental teaching aid for comparing the respiration mode of yeast cells using the following technical solution:
[0006] An experimental teaching aid for comparing the respiration modes of yeast cells comprises a display board, an aerobic reaction bottle arranged on the display board, and an anaerobic reaction bottle arranged on the display board, wherein both the aerobic reaction bottle and the anaerobic reaction bottle are filled with yeast, the display board is provided with a nutrient adder for injecting nutrients into the aerobic reaction bottle and the anaerobic reaction bottle, and the display board is provided with a stirring device for stirring the mixed liquid in the aerobic reaction bottle and the anaerobic reaction bottle.
[0007] By adopting the above technical solution, during the experiment, nutrients are injected into the aerobic reaction bottle and the anaerobic reaction bottle through the nutrient adder, and the mixed liquid (yeast culture liquid) in the aerobic reaction bottle and the anaerobic reaction bottle is stirred and mixed by the stirring device, so that the yeast and the nutrients are fully mixed and contacted, and the reaction speed of the aerobic respiration reaction and the anaerobic respiration reaction is accelerated, thereby reducing the experimental time.
[0008] Preferably, the stirring device includes a magnetic stirrer arranged on the display board and located below the aerobic reaction bottle / anaerobic reaction bottle, and a stirring magnet built into the bottom of the bottle cavity of the aerobic reaction bottle / anaerobic reaction bottle.
[0009] By adopting the above technical solution, after nutrients are injected into the aerobic reaction bottle and the anaerobic reaction bottle through the nutrient adder, the magnetic stirrer is started, and the magnetic field change in the magnetic stirrer drives the stirring magnet placed in the aerobic reaction bottle / anaerobic reaction bottle to rotate in a circle, thereby achieving the purpose of stirring the mixed liquid.
[0010] Preferably, it also includes a gas processing device, which includes an air pump, a carbon dioxide elimination bottle and a carbon dioxide detection bottle arranged on the display board, an air inlet pipe is connected between the air pump and the carbon dioxide elimination bottle, the carbon dioxide elimination bottle is filled with sodium hydroxide solution, the carbon dioxide detection bottle is filled with bromothymol blue solution, the carbon dioxide elimination bottle and the carbon dioxide detection bottle are connected with an elimination outlet pipe, and the carbon dioxide elimination bottle and the aerobic reaction bottle are connected with an aerobic inlet pipe.
[0011] By adopting the above technical scheme, an air pump is used to input air into the carbon dioxide elimination bottle through the air inlet pipe. After the sodium hydroxide solution in the carbon dioxide elimination bottle absorbs the carbon dioxide in the air, it is input into the carbon dioxide detection bottle through the elimination outlet pipe. The bromothymol blue solution in the carbon dioxide detection bottle is used to detect the absorption and elimination of carbon dioxide, ensuring that carbon dioxide-free air is continuously passed through the aerobic reaction bottle, creating aerobic conditions for aerobic respiration of yeast while eliminating the interference of carbon dioxide on the experimental results.
[0012] Preferably, a bubble stone immersed in the liquid in the carbon dioxide elimination bottle is provided at the end of the air inlet pipe.
[0013] By adopting the above technical solution, the gas from the air inlet pipe is passed into the sodium hydroxide solution in the carbon dioxide elimination bottle through the bubble stone, so that the carbon dioxide in the air is fully in contact with the sodium hydroxide solution, thereby improving the thoroughness of eliminating the carbon dioxide in the air.
[0014] Preferably, the gas processing device further comprises a balloon filled with inert gas, an inert gas inlet pipe is connected between the balloon and the anaerobic reaction bottle, and the inert gas inlet pipe is provided with a first control valve.
[0015] By adopting the above technical solution, the first control valve is opened, and the air in the anaerobic reaction bottle is replaced by the inert gas in the balloon, thereby creating anaerobic conditions for anaerobic respiration of yeast.
[0016] Preferably, a gas product detection device is also included, which includes a pair of gas product detection bottles and a liquid-sealed bottle arranged on a display board, the gas product detection bottle is filled with a bromothymol blue solution, and the liquid-sealed bottle is filled with a solution, a first gas product outlet pipe is connected between one of the gas product detection bottles and the aerobic reaction bottle, and a second gas product outlet pipe is connected between the other gas product detection bottle and the anaerobic reaction bottle, the gas outlet ends of the first gas product outlet pipe and the second gas product outlet pipe are both immersed in the bromothymol blue solution, the two gas product detection bottles are both provided with a liquid-sealed inlet pipe connected to the liquid-sealed bottle, and the liquid-sealed bottle is connected to an exhaust pipe located above the solution.
[0017] By adopting the above technical scheme, the gas generated by the aerobic reaction bottle / anaerobic reaction bottle is passed into the gas product detection bottle through the first gas product outlet pipe / the second gas product outlet pipe, and the color change of the bromothymol blue solution in the gas product detection bottle is observed. Finally, the gas enters the solution in the liquid-sealed bottle through the liquid-sealed inlet pipe and is then discharged through the outlet pipe. The additional liquid-sealed pipe can effectively prevent carbon dioxide in the outside air from entering the gas product detection bottle and causing interference.
[0018] Preferably, the gas outlet ends of the first gas product outlet pipe and the second gas product outlet pipe are both provided with bubble stones immersed in bromothymol blue solution.
[0019] By adopting the above technical solution, the gas outlet ends of the first gas product outlet pipe and the second gas product outlet pipe are both provided with bubble stones immersed in the bromothymol blue solution, so that the carbon dioxide generated by the reaction can fully contact the bromothymol blue solution, allowing the bromothymol blue solution to react quickly.
[0020] Preferably, the display board is provided with a sodium hydroxide adder for injecting sodium hydroxide solution into the gas product detection bottle.
[0021] By adopting the above technical scheme, when the bromothymol blue solution in the gas product detection bottle has reacted with carbon dioxide to turn yellow, a sodium hydroxide solution is injected into the gas product detection bottle using a sodium hydroxide adder, and is added into the gas product detection bottle in small amounts and multiple times until the bromothymol blue solution turns blue, so as to facilitate use in the next experiment and realize the reuse of the bromothymol blue solution.
[0022] Preferably, an alcohol detection device is also included, which includes a pair of alcohol detection bottles arranged on a display board, the alcohol detection bottles are filled with acidic orange potassium dichromate solution, a first culture fluid outlet pipe is connected between one of the alcohol detection bottles and the aerobic reaction bottle, a second culture fluid outlet pipe is connected between the other alcohol detection bottle and the anaerobic reaction bottle, the first gas product outlet pipe and the second gas product outlet pipe are both provided with a second control valve for controlling passage, and the first culture fluid outlet pipe and the second culture fluid outlet pipe are both provided with a third control valve for controlling passage.
[0023] By adopting the above technical scheme, when the gas product is detected, the second control valve is opened and the third control valve is closed, and the gas generated by the aerobic reaction bottle / anaerobic reaction bottle passes through the first gas product outlet pipe / the second gas product outlet pipe into the gas product detection bottle for detection; when the alcohol is detected and compared, the second control valve is closed and the third control valve is opened, and the yeast culture solution in the aerobic reaction bottle / anaerobic reaction bottle passes through the first culture solution outlet pipe / the second culture solution outlet pipe into the alcohol detection bottle, and the anaerobic respiration will produce alcohol and react with the acidic orange potassium dichromate solution, and the alcohol detection bottle connected to the anaerobic reaction bottle will change color, and the alcohol detection bottle connected to the aerobic reaction bottle will not change color, thereby forming a contrast.
[0024] Preferably, the alcohol detection device also includes a pair of culture liquid filter bottles arranged on the display board, the culture liquid filter bottles are connected to the first culture liquid outlet pipe / the second culture liquid outlet pipe, a filter tube is connected between the culture liquid filter bottle and the alcohol detection bottle, and a filter layer for removing impurities and pigments in the yeast culture liquid is arranged in the culture liquid filter bottle.
[0025] By adopting the above technical solution, the yeast culture solution has color, which will interfere with the detection of alcohol. When testing alcohol, the yeast culture solution first enters the culture solution filter bottle through the first culture solution outlet tube / the second culture solution outlet tube, and is filtered through the filter layer to remove impurities and pigments in the yeast culture solution. The clear filtrate flows into the alcohol detection bottle through the filter tube for detection, so that the color change in the alcohol detection bottle can be clearly observed.
[0026] In summary, the utility model has the following beneficial effects:
[0027] 1. During the experiment, after the nutrients are injected into the aerobic reaction bottle and the anaerobic reaction bottle through the nutrient adder, the mixed solution (yeast culture solution) in the aerobic reaction bottle and the anaerobic reaction bottle is stirred and mixed through the stirring device, so that the yeast and the nutrients are fully mixed and contacted, and the reaction speed of the aerobic respiration reaction and the anaerobic respiration reaction is accelerated, thereby reducing the experimental time;
[0028] 2. When the bromothymol blue solution in the gas product detection bottle has reacted with carbon dioxide to turn yellow, use a sodium hydroxide adder to inject sodium hydroxide solution into the gas product detection bottle, and add it to the gas product detection bottle in small amounts and multiple times until the bromothymol blue solution turns blue, so as to facilitate the use of the next experiment and realize the reuse of the bromothymol blue solution;
[0029] 3. The yeast culture fluid is colored and will interfere with the detection of alcohol. When testing alcohol, the yeast culture fluid first enters the culture fluid filter bottle through the first culture fluid outlet tube / the second culture fluid outlet tube. After filtering through the filter layer, impurities and pigments in the yeast culture fluid are removed, and the clear filtrate flows into the alcohol detection bottle through the filter tube for detection, so that the color changes in the alcohol detection bottle can be clearly observed. BRIEF DESCRIPTION OF THE DRAWINGS
[0030] Figure 1 It is a schematic diagram of the overall structure of an experimental teaching aid for comparing the respiration modes of yeast cells in Example 1;
[0031] Figure 2 is a schematic structural diagram of the gas processing device in Example 1;
[0032] Figure 3 is a schematic structural diagram of the gas product detection device in Example 1;
[0033] Figure 4 is a schematic structural diagram of the alcohol detection device in Example 1;
[0034] Figure 5 Schematic diagram of the structure of the stirring bracket in Example 2.
[0035] In the figure, 1, display board; 11, nutrient injection syringe; 12, first control switch; 13, second control switch; 14, throat clamp; 15, sodium hydroxide injection syringe; 16, potassium dichromate injection syringe; 17, handle; 2, aerobic reaction bottle; 21, first guide tube; 3, anaerobic reaction bottle; 31, anaerobic inlet pipe; 4, gas treatment device; 41, air pump; 42, carbon dioxide elimination bottle; 43, carbon dioxide detection bottle; 44, balloon; 45, air inlet pipe; 451, one-way valve; 46, elimination outlet pipe; 47, aerobic inlet pipe; 48, inert gas inlet pipe; 481, first control valve; 5, gas product detection device; 51, gas product detection bottle; 52, liquid seal bottle; 521, exhaust pipe; 53, second guide tube tube; 54, first gas product outlet pipe; 55, second gas product outlet pipe; 56, three-way joint; 57, drain pipe; 58, second control valve; 59, liquid seal inlet pipe; 50, third conduit; 6, alcohol detection device; 61, alcohol detection bottle; 62, culture medium filter bottle; 63, first culture medium outlet pipe; 64, second culture medium outlet pipe; 65, third control valve; 66, filter layer; 661, upper cotton layer; 662, activated carbon layer; 663, sand layer; 664, lower cotton layer; 67, filter tube; 68, hose; 7, stirring device; 71, stirring bracket; 711, support seat; 712, sliding seat; 72, magnetic stirrer; 73, stirring magnet; 74, guide rod; 75, adjusting screw; 76, knob. DETAILED DESCRIPTION
[0036] The following is combined with Figure 1-5 This application is described in further detail.
[0037] Embodiment 1:
[0038] The present application example discloses an experimental teaching aid for comparing the respiratory modes of yeast cells, referring to Figure 1 , including a display board 1, an aerobic reaction bottle 2, an anaerobic reaction bottle 3, a gas processing device 4, a gas product detection device 5 and an alcohol detection device 6. In this embodiment, the display board 1 is an acrylic board, and the aerobic reaction bottle 2 and the anaerobic reaction bottle 3 are both filled with yeast.
[0039] Reference Figure 1 , Figure 2 The gas treatment device 4 includes an air pump 41 fixedly connected to the display board 1, a carbon dioxide elimination bottle 42, a carbon dioxide detection bottle 43 and a balloon 44 filled with inert gas. The display board 1 is provided with a first control switch 12 for controlling the start and stop of the air pump 41. The carbon dioxide elimination bottle 42 is filled with a 10% sodium hydroxide solution. An air inlet pipe 45 is connected between the air pump 41 and the carbon dioxide elimination bottle 42. The air inlet pipe 45 is provided with a one-way valve 451. The end of the air inlet pipe 45 is provided with a bubble stone immersed in the liquid in the carbon dioxide elimination bottle 42. The carbon dioxide detection bottle 43 is filled with bromothymol blue solution. An elimination outlet pipe 46 is connected between the carbon dioxide elimination bottle 42 and the carbon dioxide detection bottle 43. One end of the elimination outlet pipe 46 is located above the liquid in the carbon dioxide elimination bottle 42, and the other end of the elimination outlet pipe 46 is immersed in the liquid in the carbon dioxide detection bottle 43. An aerobic inlet pipe 47 is connected between the carbon dioxide elimination bottle 42 and the aerobic reaction bottle 2 , one end of the aerobic inlet pipe 47 is located above the liquid in the carbon dioxide detection bottle 43 , and the other end of the aerobic inlet pipe 47 is immersed in the liquid in the aerobic reaction bottle 2 .
[0040] Reference Figure 1 , Figure 3 An inert gas inlet pipe 48 is connected between the balloon 44 and the anaerobic reaction bottle 3, and the inert gas inlet pipe 48 is provided with a first control valve 481, and the first control valve 481 is a two-way control valve. In this embodiment, the top wall of the anaerobic reaction bottle 3 is fixedly penetrated with an anaerobic inlet pipe 31, and the lower end of the anaerobic inlet pipe 31 is immersed in the liquid of the anaerobic reaction bottle 3. The upper part of the inert gas inlet pipe 48 and the anaerobic inlet pipe 31 are locked by a joint thread, so as to realize the detachable connection between the inert gas inlet pipe 48 and the anaerobic inlet pipe 31.
[0041] The display board 1 is provided with a nutrient adder for injecting nutrients into the aerobic reaction bottle 2 and the anaerobic reaction bottle 3. The nutrient is 10 ml of 5% glucose solution. The nutrient adder is a nutrient injection syringe 11. In the present embodiment, the top walls of the aerobic reaction bottle 2 and the anaerobic reaction bottle 3 are fixedly penetrated with a first conduit 21. The lower end of the first conduit 21 is located above the solution of the aerobic reaction bottle 2 / anaerobic reaction bottle 3. The syringe head of the nutrient injection syringe 11 and the upper end of the first conduit 21 are locked by a joint thread to realize a detachable connection between the nutrient injection syringe 11 and the first conduit 21. The needle head of the nutrient injection syringe 11 is penetrated by the first conduit 21 and is located above the solution of the aerobic reaction bottle 2 / anaerobic reaction bottle 3.
[0042] The display board 1 is provided with a stirring device 7 for stirring the mixed liquid in the aerobic reaction bottle 2 and the anaerobic reaction bottle 3. The stirring device 7 comprises a stirring bracket 71 arranged on the display board 1 and located below the aerobic reaction bottle 2 / anaerobic reaction bottle 3, a magnetic stirrer 72 fixedly connected to the stirring bracket 71, and a stirring magnet 73 built into the bottom of the bottle cavity of the aerobic reaction bottle 2 / anaerobic reaction bottle 3 to stir the yeast culture liquid in the aerobic reaction bottle 2 / anaerobic reaction bottle 3. The lower end surface of the aerobic reaction bottle 2 / anaerobic reaction bottle 3 abuts against the stirring bracket 71, and the display board 1 is fixedly connected with a throat clamp 14 for clamping and fixing the aerobic reaction bottle 2 / anaerobic reaction bottle 3. The display board 1 is provided with a second control switch 13 for controlling the start and stop of the magnetic stirrer 72.
[0043] The gas product detection device 5 includes a pair of gas product detection bottles 51 and a liquid seal bottle 52 fixedly connected to the display board 1, and the gas product detection bottle 51 is filled with bromothymol blue solution. The top wall of the gas product detection bottle 51 is fixedly penetrated with a second conduit 53, and the lower end of the second conduit 53 is provided with a bubble stone immersed in the bromothymol blue solution. A first gas product outlet pipe 54 is connected between the second conduit 53 and the aerobic reaction bottle 2 on one of the gas product detection bottles 51, one end of the first gas product outlet pipe 54 is connected to the side wall of the first conduit 21 on the aerobic reaction bottle 2, and the other end of the first gas product outlet pipe 54 is connected to the second conduit 53 through a three-way joint 56, and the three-way joint 56 is connected to an emptying pipe 57, and the three-way joint 56 is provided with a third control valve 65, and the third control valve 65 is a three-way control valve. A second gas product outlet pipe 55 is connected between the second conduit 53 on the other gas product detection bottle 51 and the anaerobic reaction bottle 3. The connection method of the second gas product outlet pipe 55 is the same as that of the first gas product outlet pipe 54, which will not be repeated here.
[0044] The liquid-sealed bottle 52 is filled with 10% sodium hydroxide solution. Both gas product detection bottles 51 are provided with a liquid-sealed inlet pipe 59 connected to the liquid-sealed bottle 52. Specifically, a third conduit 50 is fixedly penetrated through the top wall of the gas product detection bottle 51. The lower end of the third conduit 50 is located above the solution in the gas product detection bottle 51. One end of the liquid-sealed inlet pipe 59 is connected to the side wall of the third conduit 50. The other end of the liquid-sealed inlet pipe 59 is immersed in the solution of the gas product detection bottle 51. An exhaust pipe 521 located above the solution is fixedly penetrated through the top wall of the liquid-sealed bottle 52. The display board 1 is provided with a sodium hydroxide adder for injecting 1 ml of 2% sodium hydroxide solution into the gas product detection bottle 51. The sodium hydroxide adder is a sodium hydroxide injection syringe 15. The syringe head of the sodium hydroxide injection syringe 15 and the upper end of the third conduit 50 are locked by a joint thread to achieve a detachable connection between the sodium hydroxide injection syringe 15 and the third conduit 50. The needle head of the sodium hydroxide injection syringe 15 is inserted into the third conduit 50 and is located above the solution in the gas product detection bottle 51.
[0045] Reference Figure 3 , Figure 4 The alcohol detection device 6 includes a pair of alcohol detection bottles 61 fixedly connected to the display board 1 and a culture solution filter bottle 62 fixedly connected to the display board 1, and the culture solution filter bottle 62 is located above the alcohol detection bottle 61. A first culture solution outlet pipe 63 is connected between one of the culture solution filter bottles 62 and the aerobic reaction bottle 2, one end of the first culture solution outlet pipe 63 is immersed in the solution of the aerobic reaction bottle 2, and the other end of the first culture solution outlet pipe 63 is fixedly penetrated through the top wall of one of the culture solution filter bottles 62, and a second culture solution outlet pipe 64 is connected between the other culture solution filter bottle 62 and the anaerobic reaction bottle 3, one end of the second culture solution outlet pipe 64 is immersed in the solution of the anaerobic reaction bottle 3, and the other end of the second culture solution outlet pipe 64 is fixedly penetrated through the top wall of the other culture solution filter bottle 62. The first culture solution outlet pipe 63 and the second culture solution outlet pipe 64 are both provided with a third control valve 65 for controlling the opening and closing, and the third control valve 65 is a two-way control valve.
[0046] The lower part of the bottle cavity of the culture fluid filter bottle 62 is provided with a filter layer 66 for removing impurities and pigments in the yeast culture fluid. Specifically, the filter layer 66 includes an upper cotton layer 661, an activated carbon layer 662, a sand layer 663, and a lower cotton layer 664 from top to bottom. The upper part of the alcohol detection bottle 61 is open, and the lower end of the culture fluid filter bottle 62 is connected to a filter tube 67, and the lower end of the filter tube 67 extends to the bottom of the inner cavity of the alcohol detection bottle 61. The display board 1 is provided with a potassium dichromate adder for injecting an acidic orange potassium dichromate solution into the inner cavity of the alcohol detection bottle 61. The potassium dichromate adder is a potassium dichromate injection syringe 16, and the syringe head of the potassium dichromate injection syringe 16 is fixed with a hose 68 extending into the alcohol detection bottle 61. A handle 17 is fixedly connected to the display board 1, and all pipelines in this device are PVC pipes.
[0047] The implementation principle of an experimental teaching aid for comparing the respiration mode of yeast cells in the present application example is as follows:
[0048] Before the experiment, the exhaust pipe 57, the first gas product outlet pipe 54, and the second gas product outlet pipe 55 are opened through the second control valve 58, the second conduit 53 is closed, the first culture fluid outlet pipe 63 and the second culture fluid outlet pipe 64 are closed through the third control valve 65, the air pump 41 and the squeezing balloon 44 are started, and the gas in the aerobic reaction bottle 2 / anaerobic reaction bottle 3 is discharged through the first conduit 21, the first gas product outlet pipe 54 / the second gas product outlet pipe 55 and the exhaust pipe 57; after the discharge, the exhaust pipe 57 is closed through the second control valve 58, the first gas product outlet pipe 54, the second gas product outlet pipe 55 and the second conduit 53 are opened, and the inert gas inlet pipe 48 is closed through the first control valve 481;
[0049] Aerobic respiration reaction: start the air pump 41, and the air enters the carbon dioxide elimination bottle 42 through the air inlet pipe 45. The sodium hydroxide solution in the carbon dioxide elimination bottle 42 absorbs the carbon dioxide in the air and is input into the carbon dioxide detection bottle 43 through the elimination outlet pipe 46. The bromothymol blue solution in the carbon dioxide detection bottle 43 detects the absorption and elimination of carbon dioxide to ensure that the air without carbon dioxide is continuously passed through the aerobic reaction bottle 2; and nutrients are added to the aerobic reaction bottle 2 through the nutrient injection syringe 11 to carry out aerobic respiration reaction. During the reaction, start the magnetic stirrer 72 to drive the stirring magnet 73 to perform a circular rotation, thereby stirring the yeast culture solution in the aerobic reaction bottle 2. After sufficient reaction, the generated carbon dioxide enters the gas product detection bottle 51 through the first conduit 21, the first gas product outlet pipe 54, and the second conduit 53 and reacts with the bromothymol blue solution, causing the bromothymol blue solution to change color; then the first gas product outlet pipe 54 is closed through the second control valve 58, and the first culture liquid outlet pipe 63 is opened through the third control valve 65. Under the continuous pressure of the air pump 41, the yeast culture liquid in the aerobic reaction bottle 2 flows into the culture liquid filter bottle 62 through the first culture liquid outlet pipe 63, and after being filtered by the filter layer 66, it flows into the alcohol detection bottle 61 through the filter tube 67, and the acidic orange potassium dichromate solution is dripped into the alcohol detection bottle 61 through the potassium dichromate injection syringe 16.
[0050] Anaerobic respiration reaction: Nutrients are added to the anaerobic reaction bottle 3 through the nutrient injection syringe 11 to perform anaerobic respiration reaction. During the reaction, the magnetic stirrer 72 is started to drive the stirring magnet 73 to rotate in a circle, thereby stirring the yeast culture solution in the anaerobic reaction bottle 3. After sufficient reaction, the generated carbon dioxide enters the gas product detection bottle 51 through the first conduit 21, the second gas product outlet pipe 55, and the second conduit 53 and reacts with the bromothymol blue solution, causing the bromothymol blue solution to change color; then, the second gas product outlet pipe 55 is used to inject the carbon dioxide into the gas product detection bottle 51, and ... The second control valve 58 closes the second gas product outlet pipe 55, opens the second culture fluid outlet pipe 64 through the third control valve 65, and opens the inert gas inlet pipe 48 through the first control valve 481, squeezes the balloon 44, and under the continuous pressurization of the balloon 44, the yeast culture fluid in the anaerobic reaction bottle 3 flows into the culture fluid filtration bottle 62 through the second culture fluid outlet pipe 64, and after being filtered by the filter layer 66, flows into the alcohol detection bottle 61 through the filter tube 67, and the acidic orange potassium dichromate solution is dripped into the alcohol detection bottle 61 through the potassium dichromate injection syringe 16.
[0051] After the experiment is completed: use the sodium hydroxide injection syringe 15 to inject sodium hydroxide solution into the gas product detection bottle 51, and add it into the gas product detection bottle 51 in small amounts and multiple times until the bromothymol blue solution turns blue, so as to facilitate the use of the next experiment and realize the reuse of the bromothymol blue solution.
[0052] Embodiment 2:
[0053] The difference from Example 1 is that, referring to Figure 5 The stirring bracket 71 includes a supporting seat 711 fixedly connected to the display board 1 and located below the aerobic reaction bottle 2 / anaerobic reaction bottle 3, and a sliding seat 712 located below the supporting seat 711 and connected to the supporting seat 711 along the vertical sliding direction. The upper end surface of the supporting seat 711 abuts against the lower end surface of the aerobic reaction bottle 2 / anaerobic reaction bottle 3. The magnetic stirrer 72 is fixedly connected to the sliding seat 712. The lower end of the supporting seat 711 is fixedly connected with a guide rod 74 penetrating the sliding seat 712. The lower end surface of the supporting seat 711 is rotatably connected with an adjusting screw 75 threadedly penetrating the sliding seat 712. The lower end of the adjusting screw 75 is fixedly connected with a knob 76. By rotating the knob 76, the adjusting screw 75 is driven to rotate, thereby adjusting the height position of the sliding seat 712, thereby adjusting the distance between the magnetic stirrer 72 and the stirring magnet 73, so as to change the magnitude of the magnetic force on the stirring magnet 73, thereby changing the stirring speed of the stirring magnet 73.
[0054] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.
Claims
1. An experimental teaching aid for comparing the respiration modes of yeast cells, characterized in that: The invention comprises a display board (1), an aerobic reaction bottle (2) arranged on the display board (1), and an anaerobic reaction bottle (3) arranged on the display board (1); the aerobic reaction bottle (2) and the anaerobic reaction bottle (3) are both filled with yeast; the display board (1) is provided with a nutrient adder for injecting nutrients into the aerobic reaction bottle (2) and the anaerobic reaction bottle (3); and the display board (1) is provided with a stirring device (7) for stirring the mixed liquid in the aerobic reaction bottle (2) and the anaerobic reaction bottle (3).
2. An experimental teaching aid for comparing the respiration modes of yeast cells according to claim 1, characterized in that: The stirring device (7) comprises a magnetic stirrer (72) arranged on the display board (1) and located below the aerobic reaction bottle (2) / anaerobic reaction bottle (3), and a stirring magnet (73) built into the bottom of the bottle cavity of the aerobic reaction bottle (2) / anaerobic reaction bottle (3).
3. The experimental teaching aid for comparing the respiration modes of yeast cells according to claim 1, characterized in that: The invention also comprises a gas processing device (4), wherein the gas processing device (4) comprises an air pump (41), a carbon dioxide elimination bottle (42) and a carbon dioxide detection bottle (43) arranged on the display board (1); an air inlet pipe (45) is connected between the air pump (41) and the carbon dioxide elimination bottle (42); a sodium hydroxide solution is contained in the carbon dioxide elimination bottle (42); a bromothymol blue solution is contained in the carbon dioxide detection bottle (43); an elimination outlet pipe (46) is connected between the carbon dioxide elimination bottle (42) and the carbon dioxide detection bottle (43); and an aerobic inlet pipe (47) is connected between the carbon dioxide elimination bottle (42) and the aerobic reaction bottle (2).
4. The experimental teaching aid for comparing the respiration modes of yeast cells according to claim 3, characterized in that: The end of the air inlet pipe (45) is provided with a bubble stone immersed in the liquid in the carbon dioxide elimination bottle (42).
5. The experimental teaching aid for comparing the respiration modes of yeast cells according to claim 3, characterized in that: The gas processing device (4) further comprises a balloon (44) filled with an inert gas, an inert gas inlet pipe (48) is connected between the balloon (44) and the anaerobic reaction bottle (3), and the inert gas inlet pipe (48) is provided with a first control valve (481).
6. The experimental teaching aid for comparing the respiration modes of yeast cells according to claim 1, characterized in that: The invention also comprises a gas product detection device (5), the gas product detection device (5) comprising a pair of gas product detection bottles (51) and a liquid-sealed bottle (52) arranged on the display board (1); the gas product detection bottle (51) contains a bromothymol blue solution, and the liquid-sealed bottle (52) contains a solution; a first gas product outlet pipe (54) is connected between one of the gas product detection bottles (51) and the aerobic reaction bottle (2); a second gas product outlet pipe (55) is connected between the other gas product detection bottle (51) and the anaerobic reaction bottle (3); the gas outlet ends of the first gas product outlet pipe (54) and the second gas product outlet pipe (55) are both immersed in the bromothymol blue solution; the two gas product detection bottles (51) are both provided with a liquid-sealed inlet pipe (59) connected to the liquid-sealed bottle (52); and the liquid-sealed bottle (52) is connected to an exhaust pipe (521) located above the solution.
7. The experimental teaching aid for comparing the respiration modes of yeast cells according to claim 6, characterized in that: The gas outlet ends of the first gas product outlet pipe (54) and the second gas product outlet pipe (55) are both provided with air bubble stones immersed in bromothymol blue solution.
8. The experimental teaching aid for comparing the respiration modes of yeast cells according to claim 6, characterized in that: The display board (1) is provided with a sodium hydroxide adder for injecting sodium hydroxide solution into the gas product detection bottle (51).
9. The experimental teaching aid for comparing the respiration modes of yeast cells according to claim 6, characterized in that: The invention also comprises an alcohol detection device (6), wherein the alcohol detection device (6) comprises a pair of alcohol detection bottles (61) arranged on the display board (1), wherein the alcohol detection bottles (61) contain an acidic orange potassium dichromate solution, wherein a first culture fluid outlet pipe (63) is connected between one of the alcohol detection bottles (61) and the aerobic reaction bottle (2), and a second culture fluid outlet pipe (64) is connected between the other alcohol detection bottle (61) and the anaerobic reaction bottle (3), wherein the first gas product outlet pipe (54) and the second gas product outlet pipe (55) are both provided with a second control valve (58) for controlling the opening and closing, and the first culture fluid outlet pipe (63) and the second culture fluid outlet pipe (64) are both provided with a third control valve (65) for controlling the opening and closing.
10. The experimental teaching aid for comparing the respiration modes of yeast cells according to claim 9, characterized in that: The alcohol detection device (6) also includes a pair of culture fluid filter bottles (62) arranged on the display board (1), the culture fluid filter bottle (62) is connected to the first culture fluid outlet pipe (63) / the second culture fluid outlet pipe (64), a filter tube (67) is connected between the culture fluid filter bottle (62) and the alcohol detection bottle (61), and a filter layer (66) for removing impurities and pigments in the yeast culture fluid is arranged in the culture fluid filter bottle (62).