Multi-channel detection reagent card

Abstract

The utility model relates to the technical field of immunodetection, and provides a multi-channel detection reagent card which comprises a shell provided with a sample adding port; the drainage channel is arranged in the inner cavity of the shell, and the drainage channel comprises a plurality of sub-channels which are arranged in parallel; the drainage structure is arranged in the inner cavity of the shell and located between the sample adding opening and the drainage channel, the drainage structure is provided with a plurality of drainage branches arranged in the circumferential direction of the sample adding opening, and each drainage branch is arranged to be matched with one sub-channel so that samples at the sample adding opening can be drained into different sub-channels; the detection reagent strips are arranged at one end, far away from the drainage structure, of each sub-channel, and the detection reagent strips in different sub-channels can detect different target types such as bacteria, viruses and toxins at the same time. The multi-channel detection reagent card provided by the utility model can be used for detecting and screening various different virulent biological hazard factors in a sample at one time, and is beneficial to shortening the detection time and improving the detection efficiency.

Description

Technical Field

[0001] The utility model relates to the technical field of immunoassay, and particularly relates to a multi-channel detection reagent card. Background Art

[0002] Among virulent biological hazard factors, virulent pathogenic bacteria and viruses have the characteristics of high infectivity and high lethality, and virulent toxins have the risk of causing death or disability in a short time with a small amount. At customs ports, airports, railway stations and other places with large mobility and dense crowds, it is crucial to prevent the illegal carrying and spreading of virulent pathogens into the country and to conduct immediate on-site screening for sudden public health security incidents.

[0003] In terms of biological tracers, the techniques of immunochromatographic detection mainly include colloidal gold immunochromatographic detection technique, up-converting luminescence immunochromatographic technique and quantum dot immunochromatographic technique. A reagent card is required for detection. However, most of the currently used detection reagent cards are single-channel reagent cards, which can only screen one target in a suspicious sample at a time. For samples containing multiple virulent biological hazard factors, multiple detections are required, resulting in a long detection time and low detection efficiency, and it is difficult to meet the screening requirements. Summary of the Utility Model

[0004] Therefore, the technical problem to be solved by the utility model is that most of the currently used detection reagent cards are single-channel reagent cards, which can only screen one target in a suspicious sample at a time. For samples containing multiple virulent biological hazard factors, multiple detections are required, resulting in a long detection time and low detection efficiency, and it is difficult to meet the screening requirements. Thus, a multi-channel detection reagent card is provided.

[0005] To solve the above technical problem, the technical solution of the utility model is as follows:

[0006] The utility model provides a multi-channel detection reagent card, including: a housing, which has a sample adding port thereon; a drainage channel, arranged in the inner cavity of the housing, and the drainage channel includes a plurality of sub-channels arranged in parallel; a drainage structure, arranged in the inner cavity of the housing and located between the sample adding port and the drainage channel, and the drainage structure has a plurality of drainage branches arranged along the circumferential direction of the sample adding port, and each drainage branch is adapted to be arranged corresponding to one sub-channel to drain the sample at the sample adding port into different sub-channels; detection reagent strips, and each detection reagent strip is arranged at one end of each sub-channel far away from the drainage structure, and the detection reagent strips in different sub-channels can simultaneously detect different target types such as bacteria, viruses, toxins, etc.

[0007] Further, the housing includes an upper card shell and a lower card shell which are buckled with each other, and the sample adding port is arranged on the upper card shell.

[0008] Further, the drainage structure includes a drainage sheet and a drainage groove; the drainage sheet is disposed on the inner wall of the upper cartridge, the drainage groove is disposed on the inner wall of the lower cartridge, and when the upper cartridge and the lower cartridge are snapped together, the drainage sheet is inserted into the drainage groove, and the interval between two adjacent drainage sheets forms the drainage branch.

[0009] Further, a plurality of upwardly convex drainage platforms are spaced on the inner wall of the lower cartridge, and side baffles are disposed on both sides of each drainage platform, and the semi-closed space formed by the side baffles and the drainage platform forms the sub-channel.

[0010] Further, the multi-channel detection reagent card further includes a horizontal baffle disposed on the inner wall of the lower cartridge, and the horizontal baffle is located at one end of each sub-channel away from the drainage structure for restricting the movement of the detection reagent strip placed in the sub-channel.

[0011] Further, the multi-channel detection reagent card further includes a drainage pad disposed in the inner cavity of the housing, and the drainage pad includes a main body and a plurality of guiding portions connected to the main body; the main body is disposed opposite to the sample adding port; the guiding portions extend into the sub-channels through the drainage branches and are connected to the detection reagent strips to drain the sample added from the sample adding port to the detection reagent strips.

[0012] Further, limiting posts are disposed at positions on the inner wall of the upper cartridge adapted to the detection reagent strips and the drainage pad, and when the upper cartridge and the lower cartridge are snapped together, the limiting posts press the detection reagent strips and the drainage pad tightly.

[0013] Further, a plurality of annular groove structures are disposed on the inner wall of the lower cartridge, and a plurality of rivet structures are disposed on the inner wall of the upper cartridge in a matching manner, and when the upper cartridge and the lower cartridge are snapped together, the rivet structures are riveted to the annular groove structures.

[0014] Further, a wide-view detection window is disposed on the upper cartridge at a position adapted to the detection reagent strips, and the horizontal dimension of the wide-view detection window is adapted to the distance of all the detection reagent strips arranged in parallel, and the vertical dimension of the wide-view detection window is adapted to the position where the analysis film of the detection reagent strip is located.

[0015] Further, the housing is a rectangular or square housing.

[0016] The technical solution of the present utility model has the following advantages:

[0017] The multi-channel detection reagent card provided by the present utility model has a plurality of sub-channels arranged in the inner cavity of the housing. During use, after adding a sample from the sample adding port, the sample is drained through the drainage branch into different sub-channels. Since the detection reagent strips in different sub-channels have different target types, it is possible to detect and screen a variety of different virulent biological hazard factors in the sample at one time, which is beneficial to shortening the detection time and improving the detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the specific embodiments of the present utility model or the technical solutions in the prior art, the following will briefly introduce the drawings required for use in the description of the specific embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0019] Figure 1 Schematic diagram of the upper card shell (reverse side) of the multi-channel detection reagent card in the embodiment of the present utility model;

[0020] Figure 2 Schematic diagram of the upper card shell (front side) of the multi-channel detection reagent card in the embodiment of the present utility model;

[0021] Figure 3 Schematic diagram of the lower card shell of the multi-channel detection reagent card in the embodiment of the present utility model;

[0022] Figure 4 Schematic diagram of the detection reagent strip of the multi-channel detection reagent card in the embodiment of the present utility model.

[0023] 1. Upper card shell; 2. Lower card shell; 301. Sub-channel; 302. Drainage branch; 4. Detection reagent strip; 5. Ring groove structure; 6. Rivet structure; 7. Sample adding port; 8. Wide-view detection window; 9. Limit post; 10. Drainage sheet; 11. Drainage groove; 12. Horizontal baffle; 13. Drainage platform; 14. Side baffle; 15. Sample pad; 16. Marking pad; 17. Analytical membrane; 18. Power pad; 19. Adhesive bottom plate; 20. Biological capture probe; 21. Biological tracer; 22. Capture antibody A; 23. Detection band; 24. Quality control band; 25. Capture antibody B; 26. Secondary antibody. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0024] The following will clearly and completely describe the technical solutions of the present utility model in conjunction with the drawings. Obviously, the described embodiments are some, but not all, of the embodiments of the present utility model. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the protection scope of the present utility model.

[0025] In the description of the present utility model, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation to the present utility model. In addition, the terms "first", "second", "third" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance.

[0026] In the description of the present utility model, it should be noted that unless otherwise clearly specified and defined, the terms "installed", "connected", "coupled" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0027] In addition, the technical features involved in different embodiments of the present utility model described below can be combined with each other as long as they do not conflict with each other.

[0028] Such as Figures 1 to 3As shown in the figure, this embodiment provides a multi-channel detection reagent card, including: a housing with a sample addition port 7 thereon; a drainage channel disposed in the inner cavity of the housing, the drainage channel including a plurality of sub-channels 301 arranged in parallel; a drainage structure disposed in the inner cavity of the housing and located between the sample addition port 7 and the drainage channel, the drainage structure having a plurality of drainage branches 302 arranged along the circumferential direction of the sample addition port 7, and each drainage branch 302 is adapted to be arranged corresponding to a sub-channel 301 to drain the sample at the sample addition port 7 into different sub-channels 301; and detection reagent strips 4, with a detection reagent strip 4 provided at one end of each sub-channel 301 away from the drainage structure, and the detection reagent strips 4 in different sub-channels 301 having different target types. Among them, the number of sub-channels 301 included in the drainage channel can be between ten and twenty. For example, the drainage channel can include ten sub-channels 301. Correspondingly, the drainage structure includes ten drainage branches 302, and the ten drainage branches 302 are a cluster. At this time, this multi-channel detection reagent card can detect and screen ten highly virulent biological hazard factors in one go. Among them, the target types of the detection reagent strips 4 include but are not limited to Bacillus anthracis spores, Yersinia pestis, Francisella tularensis, Burkholderia pseudomallei, Brucella, Escherichia coli O157, Vibrio cholerae O1 serotype, Vibrio cholerae O139 serotype, Staphylococcal enterotoxin B (SEB), ricin, abrin, Shiga toxin I, Shiga toxin II, diphtheria toxin, tetanus toxin, botulinum toxin type A, T2 toxin, Zika virus, adenovirus, Ebola virus, etc.

[0029] For the multi-channel detection reagent card provided in this embodiment, a plurality of sub-channels 301 are provided in the inner cavity of the housing. During use, after adding the sample through the sample addition port 7, the sample is drained into different sub-channels 301 through the drainage branches 302. Since the detection reagent strips 4 in different sub-channels 301 have different target types, multiple different highly virulent biological hazard factors in the sample can be detected and screened at one time, which is beneficial to shortening the detection time and improving the detection efficiency.

[0030] For example, the housing can be a rectangular or square housing. Among them, the housing includes an upper card shell 1 and a lower card shell 2 that are snap-fitted together, and the sample addition port 7 is provided on the upper card shell 1. For example, the length range of the sample addition port 7 can be 10 mm - 20 mm, and the width range can be 0.5 mm - 1.0 mm.

[0031] Among them, the drainage structure includes a drainage sheet 10 and a drainage groove 11; the drainage sheet 10 is arranged on the inner wall of the upper cartridge 1, and the drainage groove 11 is arranged on the inner wall of the lower cartridge 2. When the upper cartridge 1 and the lower cartridge 2 are buckled, the drainage sheet 10 is inserted into the drainage groove 11, and the interval between two adjacent drainage sheets 10 forms a drainage branch 302. Among them, the drainage sheet 10 can be vertical, and the shape of the drainage groove 11 is adapted to that of the drainage sheet 10. One end of the drainage sheet 10 is docked with the downstream sub-channel 301, and the other end is docked with the upstream sampling port 7, so as to facilitate the more fluent flow of the sample at the sampling port 7 into the drainage branch 302.

[0032] Among them, a number of upwardly convex drainage platforms 13 are arranged at intervals on the inner wall of the lower cartridge 2, and side baffles 14 are arranged on both sides of each drainage platform 13. The semi-closed space formed by the side baffles 14 and the drainage platform 13 forms a sub-channel 301. With such a setting, the upwardly convex drainage platform 13 and the side baffles 14 on both sides can not only prevent the sample liquid in two adjacent sub-channels 301 from interfering with each other, but also play a role in limiting the test strip 4 placed in the sub-channel 301 to prevent the test strip 4 from shaking left and right.

[0033] Among them, the multi-channel test reagent card further includes a horizontal baffle 12, which is arranged on the inner wall of the lower cartridge 2. The ends of the respective sub-channels 301 away from the drainage structure are flush, and the horizontal baffle 12 is located at the ends of the respective sub-channels 301 away from the drainage structure, and is used to limit the movement of the test strip 4 placed in the sub-channel 301. With such a setting, the horizontal baffle 12 limits the test strip 4 to prevent it from moving upward.

[0034] Among them, the multi-channel test reagent card further includes a drainage pad, which is arranged in the inner cavity of the housing. The drainage pad includes a main body and a plurality of guiding parts connected to the main body; the main body is arranged opposite to the sampling port 7; the guiding parts extend into the sub-channel 301 through the drainage branch 302 and are connected to the test strip 4 to drain the sample added from the sampling port 7 to the test strip 4. When the sample is added from the sampling port 7, it will drop on the main body, and then the guiding parts can transport the sample to the downstream test strip 4 by means of adsorption. For example, the guiding part is connected to the sample pad 15 of the test strip 4.

[0035] Among them, limit posts 9 are arranged at positions on the inner wall of the upper cartridge 1 that are adapted to the test strip 4 and the drainage pad. When the upper cartridge 1 and the lower cartridge 2 are buckled with each other, the limit posts 9 press the test strip 4 and the drainage pad tightly. For example, the number of limit posts 9 can be designed according to needs. The limit posts can be columnar bodies in the shape of a cuboid or a cylinder.

[0036] Among them, a plurality of annular groove structures 5 are provided on the inner wall of the lower cartridge case 2. For example, the annular groove structures 5 can be arranged near the edge position of the lower cartridge case 2. A plurality of rivet structures 6 are correspondingly provided on the inner wall of the upper cartridge case 1. When the upper cartridge case 1 and the lower cartridge case 2 are buckled with each other, the rivet structures 6 are riveted and connected with the annular groove structures 5. It is set in this way to enable the upper cartridge case 1 and the lower cartridge case 2 to be firmly buckled. Of course, the upper cartridge case 1 and the lower cartridge case 2 can also be fixed by screws.

[0037] Among them, a wide-view detection window 8 is provided on the upper cartridge case 1 at a position adapted to the detection reagent strip 4. The horizontal dimension of the wide-view detection window 8 is adapted to the distance of all the detection reagent strips 4 arranged in parallel, and the vertical dimension of the wide-view detection window 8 is adapted to the position where the analytical membrane 17 of the detection reagent strip 4 is located. It is set in this way to facilitate observing the chromatography state and the result of the final detection.

[0038] As Figure 4 shown, among them, for the detection reagent strip 4, it includes a sticky bottom plate 19 and a sample pad 15, a labeling pad 16, an analytical membrane 17 and a power pad 18 provided thereon. Among them, the position of the wide-view detection window 8 matches that of the analytical membrane 17.

[0039] Among them, the sample pad 15 can be, but is not limited to, one of absorbent paper, cellulose membrane, glass fiber, non-woven fabric and blood filtration membrane.

[0040] Among them, the labeling pad 16 can be, but is not limited to, one of glass fiber, polyester film and non-woven fabric; a biological capture probe 20 is fixed in the labeling pad 16; the biological capture probe 20 is coupled by a biological tracer 21 as a tracer and a capture antibody A22 with the function of directionally capturing the antigen to be detected.

[0041] Among them, the analytical membrane 17 can be, but is not limited to, a nitrocellulose membrane or a nylon membrane; among them, a test band 23 and a quality control band 24 are provided on the analytical membrane 17; the test band 23 is a capture antibody B25 with the function of directionally capturing the antigen to be detected, and the quality control band is a secondary antibody 26 of a certain target capture antibody A22; the certain target capture antibody B25 on the test band 23 can form a double-antibody sandwich mode with the certain target capture antibody A22 as a liquid-phase recognition probe in the biological capture probe 20 to specifically detect a certain target, and the quality control band 24 can be directly combined with the biological capture probe 20 to control whether the entire chromatography process is normal.

[0042] Among them, the power pad 18 can be, but is not limited to, absorbent paper or cellulose membrane.

[0043] Among them, the adhesive bottom plate 19 is a rigid PVC plate with pressure-sensitive adhesive coated on one side, which can make the sample pad 15, the marking pad 16, the analysis film 17, and the power pad 18 overlap and fix each other in a certain way on the adhesive bottom plate 19, ensuring the continuity of the liquid flow inside the single-target detection reagent strip 4.

[0044] Among them, the biological capture probe 20 can indicate the presence and concentration of a certain target in the sample through the phenomenon of generating fluorescence with a specific wavelength under ultraviolet light excitation.

[0045] Among them, a certain target capture antibody A and a certain target capture antibody B can be monoclonal antibodies or polyclonal antibodies, and can be the same or different.

[0046] Among them, the highly virulent biological hazard factor is an immunochromatographic biological capture probe 20 formed by covalently coupling a biological tracer 21 based on quantum dots, lanthanide elements and their chelating agents, colloidal gold, and upconversion luminescent particles with a specific biological recognition probe to provide a quantitative detection of the fluorescence source.

[0047] The detection method is as follows:

[0048] Sample pretreatment: Water samples, food, and fecal samples are directly detected after preliminary separation and enrichment.

[0049] Processing the sample: Mix 1 volume of the sample with 1 volume of the general sample treatment solution evenly. The general sample treatment solution is PB with a pH of 7.2 and a concentration of 0.03M, containing 0.021M of Na 2 HPO 4 and 0.009M of NaH 2 PO 4 , and 1% SDS.

[0050] Adding the sample: Place the reagent card on a horizontal tabletop and add 1.5 mL of the processed sample to the sample addition port 7.

[0051] Chromatographic reaction: Place the reagent card after adding the sample on a horizontal tabletop and let it stand for 10 min until the chromatographic reaction is completed.

[0052] Result interpretation: Put the reagent card into the quantum dot detector, perform a one-time scanning imaging and calculate the concentration, and remove the background interference and display the detection value according to the ratio of the test band / quality control band 24.

[0053] Among them, the quality control band 24 has a value, and the result is determined to be valid. If the value of the quality control band 24 is 0, the result is determined to be invalid.

[0054] Quantitative result: In the valid results, the ratio of the sample test band / quality control band 24 represents the detected amount of a certain target, and the concentration of a certain target is calculated by fitting with the built-in standard curve, and finally the numerical quantitative results of ten targets are displayed.

[0055] In summary, the multi-channel detection reagent card in this application, with the structural design of the multi-channel detection reagent card and combined with the single-target detection reagent strip 4, realizes the quantitative detection of more than 10 virulent biological hazard factor targets simultaneously within 10 minutes with a single sample loading, meeting the on-site and multi-target requirements; based on the design of the multi-parallel channel detection reagent card structure and relying on the characteristics of the high quantum yield biological tracer 21, it meets the requirements of the immunochromatographic detection structure while achieving high space utilization, laying a foundation for handheld and portable detection devices.

[0056] Obviously, the above embodiments are merely examples given for clear illustration and are not limitations on the implementation manners. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to enumerate all the implementation manners here. And the obvious changes or modifications derived therefrom still fall within the protection scope of the present utility model.

Claims

1. A multi-channel detection reagent card, characterized in that: include: A housing, wherein the housing has a sample addition port (7); A drainage channel is arranged in the inner cavity of the shell, and the drainage channel includes a plurality of sub-channels (301) arranged in parallel; a drainage structure, arranged in the inner cavity of the shell and located between the sample addition port (7) and the drainage channel, the drainage structure having a plurality of drainage branches (302) arranged along the circumferential direction of the sample addition port (7), each of the drainage branches (302) being adapted to be arranged in one of the sub-channels (301) so as to drain the sample at the sample addition port (7) into different sub-channels (301); A detection reagent strip (4), wherein the detection reagent strip (4) is disposed at one end of each subchannel (301) away from the drainage structure, and the detection reagent strips (4) in different subchannels (301) can simultaneously detect different target types such as bacteria, viruses, and toxins; Also included is a drainage pad, which is disposed in the inner cavity of the shell, and the drainage pad includes a main body and a plurality of guide parts connected to the main body; The main body is arranged facing the sample adding port (7); The guide portion extends into the sub-channel (301) through the drainage branch (302) and is connected to the detection reagent strip (4) to drain the sample added to the sample addition port (7) to the detection reagent strip (4).

2. The multi-channel detection reagent card according to claim 1, characterized in that: The housing comprises an upper card shell (1) and a lower card shell (2) which are engaged with each other, and the sample addition port (7) is arranged on the upper card shell (1).

3. The multi-channel detection reagent card according to claim 2, characterized in that: The drainage structure comprises a drainage sheet (10) and a drainage groove (11); The drainage sheet (10) is arranged on the inner wall of the upper card shell (1), and the drainage groove (11) is arranged on the inner wall of the lower card shell (2). When the upper card shell (1) and the lower card shell (2) are buckled together, the drainage sheet (10) is inserted into the drainage groove (11), and the interval between two adjacent drainage sheets (10) forms the drainage branch (302).

4. The multi-channel detection reagent card according to claim 2, characterized in that: A plurality of upwardly convex guide platforms (13) are arranged at intervals on the inner wall of the lower casing (2), and side baffles (14) are arranged on both sides of each guide platform (13). The semi-enclosed space enclosed by the side baffles (14) and the guide platform (13) forms the sub-channel (301).

5. The multi-channel detection reagent card according to claim 4, characterized in that: It also includes a horizontal baffle (12) arranged on the inner wall of the lower casing (2), and the horizontal baffle (12) is located at one end of each sub-channel (301) away from the drainage structure, and is used to limit the movement of the detection reagent strip (4) placed in the sub-channel (301).

6. The multi-channel detection reagent card according to claim 2, characterized in that: Limiting posts (9) are provided on the inner wall of the upper card shell (1) at positions matching the detection reagent strip (4) and the drainage pad. When the upper card shell (1) and the lower card shell (2) are mutually buckled, the limiting posts (9) press the detection reagent strip (4) and the drainage pad.

7. The multi-channel detection reagent card according to claim 2, characterized in that: A plurality of annular groove structures (5) are arranged on the inner wall of the lower card shell (2), and a plurality of rivet structures (6) are arranged on the inner wall of the upper card shell (1) in a matching manner. When the upper card shell (1) and the lower card shell (2) are buckled together, the rivet structures (6) and the annular groove structures (5) are riveted together.

8. The multi-channel detection reagent card according to claim 2, characterized in that: A wide-view detection window (8) is provided on the upper card shell (1) at a position adapted to the detection reagent strip (4); the lateral dimension of the wide-view detection window (8) is adapted to the distance between all the detection reagent strips (4) arranged in parallel; and the longitudinal dimension of the wide-view detection window (8) is adapted to the position of the analysis membrane (17) of the detection reagent strip (4).

9. The multi-channel detection reagent card according to claim 1, characterized in that: The shell is a rectangular or square shell.

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