NK cell cryopreservation equipment

Abstract

The utility model discloses NK (Natural Killer) cell cryopreservation equipment which comprises a cryopreservation box and test tubes, a plurality of placing plates are transversely and slidably arranged in the cryopreservation box from top to bottom, sliding grooves are horizontally and longitudinally formed in the left side wall and the right side wall in the cryopreservation box and correspond to the placing plates respectively, and limiting sliding rails are arranged on the left side and the right side of each placing plate and correspond to the sliding grooves respectively; a handle is fixedly arranged on the front side of the placing plate, a plurality of placing grooves for placing test tubes are uniformly formed in the placing plate, arc-shaped clamping blocks are arranged at the positions, corresponding to the test tubes, of the left and right sides in the placing grooves respectively, supporting blocks are further arranged at the bottoms of the placing grooves, and supporting structures are movably arranged at the positions, corresponding to the supporting blocks, in the placing plate; the supporting structure is fixedly connected with the supporting block and is in driving connection with the arc-shaped clamping block; the supporting blocks and the arc-shaped clamping blocks are simultaneously driven through the supporting structure, the whole structure is simple, the test tubes can be stably clamped, meanwhile, operation is convenient, and practicability is high.

Description

Technical Field

[0001] The utility model relates to the technical field of cell cryopreservation, in particular to an NK cell cryopreservation device. Background Technique

[0002] NK cells, namely natural killer cells, are an important cell type in the human immune system. They belong to a type of lymphocytes and mainly exist in blood, lymphoid tissues and certain organs. The main function of NK cells is to identify and kill virus-infected cells and certain cancer cells. Different from T cells that need to identify specific antigens in advance, NK cells can directly identify and attack abnormal cells, so they are called "natural" killers.

[0003] During the research process of NK cells, it is usually necessary to cryopreserve them to ensure their activity. At present, when cryopreserving NK cells, they are usually placed in a test tube and then placed in a cryopreservation box.

[0004] When the existing cryopreservation equipment is performing cryopreservation work, it is difficult to firmly fix the test tube. When shaken, the test tube is easy to shake and collide, resulting in a broken phenomenon. Therefore, an NK cell cryopreservation device is provided to solve the above problems. Content of the Utility Model

[0005] Aiming at the above technical problems in the prior art, the utility model provides an NK cell cryopreservation device with the aim of solving the above problems.

[0006] To achieve the above object, the utility model provides the following technical solutions:

[0007] An NK cell cryopreservation device includes a cryopreservation box and a test tube. A plurality of placement plates are horizontally slidably arranged in the cryopreservation box from top to bottom. A plurality of placement grooves for placing test tubes are evenly formed on the placement plates. Arc-shaped clamping blocks are respectively arranged on the left and right sides corresponding to the test tubes in the placement grooves. Support blocks are further arranged at the bottoms of the plurality of placement grooves. A support structure is movably arranged in the placement plate corresponding to the plurality of support blocks. The support structure is fixedly connected to the support blocks and is drivingly connected to the arc-shaped clamping blocks.

[0008] Preferably, the support structure includes a plurality of support rods which are respectively vertically arranged on the lower side surface of the support block and fixedly connected to the lower side of the support block 5. The plurality of support rods are slidably connected to the placement plate. An activity cavity is correspondingly formed at the bottom of the placement plate for the plurality of support rods. A movable plate is horizontally arranged in the activity cavity. The movable plate is slidably connected to the activity cavity. The upper side surface of the movable plate is fixedly connected to the bottoms of the plurality of support rods. A threaded rod is vertically arranged at the lower side of the placement plate corresponding to the movable plate. The threaded rod passes through the bottom of the placement plate and extends into the movable plate and is rotatably connected thereto. The threaded rod is in threaded connection with the placement plate.

[0009] Preferably, an annular limiting block is arranged at the end of one end of the threaded rod located in the movable plate. An annular limiting groove is correspondingly formed in the movable plate for the annular limiting block. The annular limiting block is rotatably connected to the annular limiting groove.

[0010] Preferably, abutting blocks are respectively arranged on the opposite sides of the two arc-shaped clamping blocks. First activity grooves are respectively formed in the placement plate corresponding to the two abutting blocks. Springs are respectively and horizontally fixedly arranged on the opposite sides of the two abutting blocks. The other ends of the springs abut against the side walls of the first activity grooves. A second activity groove is formed in the upper side of the placement plate corresponding to the support block. The lower ends of the two abutting blocks respectively abut against the left and right ends of the upper side surface of the support block. Inclined surfaces which are inclined outwards from top to bottom are respectively arranged on the abutting surfaces of the abutting blocks and the support block.

[0011] Preferably, an arc-shaped positioning groove is formed in the upper side surface of the support block corresponding to the bottom of the test tube.

[0012] Preferably, sliding grooves are horizontally and longitudinally arranged on the left and right side walls in the cryogenic storage box corresponding to the plurality of placement plates. Limiting sliding rails are respectively arranged on the left and right sides of the placement plate corresponding to the sliding grooves.

[0013] Preferably, a handle is fixedly arranged on the front side of the placement plate.

[0014] Compared with the prior art, the utility model has the following beneficial effects:

[0015] When storing, for the NK cell cryogenic storage device provided by the utility model, open the cryogenic storage box, pull out the placement plate through the handle, then rotate the threaded rod to lift the movable plate upwards. Under the action of the support rods, the support block is lifted, so that the two abutting blocks are abutted outwards, and thus the two arc-shaped clamping blocks loosen the test tube and rise under the support of the support block, which is convenient for the staff to take and improves the work efficiency. At the same time, when placing, place the test tube in the arc-shaped positioning groove of the support block, and then lower the movable plate by rotating the threaded rod. Under the action of the spring, the abutting blocks are extruded to move towards each other, so that the arc-shaped clamping blocks stably clamp the test tube, avoiding the phenomenon of shaking, collision and breakage. The overall structure is simple, which can not only stably clamp the test tube, but also is convenient to operate and has high practicability. Brief Description of the Drawings

[0016] Figure 1 This is an overall schematic diagram of a NK cell cryopreservation device according to the present utility model;

[0017] Figure 2 This is an enlarged view of part A of a NK cell cryopreservation device according to the present utility model;

[0018] Figure 3 This is an enlarged view of part B of a NK cell cryopreservation device according to the present utility model.

[0019] In the figure: 1, cryopreservation box; 11, sliding groove; 2, test tube; 3, placement plate; 31, placement groove; 32, activity cavity; 33, first activity groove; 34, second activity groove; 35, limit slide rail; 36, handle; 4, arc-shaped clamping block; 41, abutting block; 42, spring; 5, support block; 51, arc-shaped positioning groove; 6, support structure; 61, support rod; 62, activity plate; 63, threaded rod; 64, annular limit block; 65, annular limit groove; 7, inclined plane. Detailed Description of the Preferred Embodiments

[0020] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0021] Please refer to Figures 1 to 3 , in the embodiments proposed in this application: A NK cell cryopreservation device includes a cryopreservation box 1 and a test tube 2. A plurality of placement plates 3 are horizontally slidably arranged in the cryopreservation box 1 from top to bottom. Corresponding to the plurality of placement plates 3, sliding grooves 11 are horizontally and longitudinally arranged on the left and right side walls of the cryopreservation box 1. Corresponding to the sliding grooves 11, limit slide rails 35 are arranged on the left and right sides of the placement plate 3. A handle 36 is fixedly arranged on the front side of the placement plate 3. A plurality of placement grooves 31 for placing the test tube 2 are evenly opened on the placement plate 3. Arc-shaped clamping blocks 4 are arranged on the left and right sides corresponding to the test tube 2 in the placement grooves 31. Support blocks 5 are further arranged at the bottoms of the plurality of placement grooves 31. A support structure 6 is movably arranged in the placement plate 3 corresponding to the plurality of support blocks 5. The support structure 6 is fixedly connected to the support block 5, and the support structure 6 is drivingly connected to the arc-shaped clamping block 4;

[0022] The support structure 6 drives the support block 5 and the arc-shaped clamping block 4 simultaneously. During placement, the support block 5 is lifted, and at the same time, the two arc-shaped clamping blocks 4 are released, facilitating the placement of the test tube 2. After placement, the support block 5 is lowered, and at the same time, the two arc-shaped clamping blocks 4 are driven to move closer to each other to clamp the test tube 2. The overall structure is simple, which can not only stably clamp the test tube 2, but also is convenient to operate and has high practicality.

[0023] In another embodiment, please refer to Figures 1 to 3 , the support structure 6 includes a plurality of support rods 61, which are respectively vertically arranged on the lower side of the support block 5 and fixedly connected to the lower side of the support block 5. The plurality of support rods 61 are slidably connected to the placement plate 3. Corresponding to the bottoms of the plurality of support rods 61, the placement plate 3 is provided with movable cavities 32. Horizontally arranged in the movable cavities 32 is a movable plate 62, which is slidably connected to the movable cavities 32. The upper side of the movable plate 62 is fixedly connected to the bottoms of the plurality of support rods 61. Corresponding to the position of the movable plate 62 on the lower side of the placement plate 3, a threaded rod 63 is vertically arranged. The threaded rod 63 passes through the bottom of the placement plate 3 and extends into the movable plate 62, and is rotatably connected thereto. At the end of the threaded rod 63 located inside the movable plate 62, there is an annular limit block 64. Corresponding to the annular limit block 64 in the movable plate 62, there is an annular limit groove 65. The annular limit block 64 is rotatably connected to the annular limit groove 65. The threaded rod 63 is threadedly connected to the placement plate 3;

[0024] Since the threaded rod 63 is threadedly connected to the placement plate 3 and rotatably connected to the movable plate 62, when the threaded rod 63 is rotated, the threaded rod 63 drives the movable plate 62 to move up and down, so that the support rods 61 on the movable plate 62 and the support block 5 move up and down, raising or lowering the test tube 2, facilitating the taking and placing of the test tube 2.

[0025] In another embodiment, please refer to Figures 1 to 3 , on the opposite sides of the two arc-shaped clamping blocks 4, there are respectively abutting blocks 41. Corresponding to the two abutting blocks 41 in the placement plate 3, there are respectively first movable grooves 33. On the opposite sides of the two abutting blocks 41, there are respectively horizontally fixed springs 42. The other ends of the springs 42 abut against the side walls of the first movable grooves 33. Corresponding to the position of the support block 5 on the upper side of the placement plate 3, there is a second movable groove 34. The lower ends of the two abutting blocks 41 respectively abut against the left and right ends of the upper side of the support block 5. The abutting blocks 41 and the support block 5 are respectively provided with inclined surfaces 7 that slope outward from top to bottom;

[0026] Since the left and right ends of the upper side of the support block 5 are in contact with the two abutting blocks 41, and the contact part is provided with an inclined surface 7, when the support block 5 rises, due to the setting of the inclined surface 7, the support block 5 drives the two abutting blocks 41 outwards, so that the arc-shaped clamping block 4 connected thereto loosens the test tube 2, and cooperates with the rise of the support block 5, so as to facilitate the staff to take it. On the contrary, when the support block 5 descends, it cooperates with the reset of the spring 42 to squeeze the two arc-shaped clamping blocks 4 towards each other, so as to clamp the test tube 2. At the same time, an arc-shaped positioning groove 51 is opened on the upper side of the support block 5 corresponding to the bottom of the test tube 2, which is convenient for accurate positioning when the test tube 2 is placed and clamped.

[0027] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprising", "including" or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not expressly listed, or also includes elements inherent to such process, method, article or device. Without further limitation, an element defined by the statement "including a..." does not exclude the existence of additional identical elements in the process, method, article or device including the said element.

[0028] In the description of the present invention, unless otherwise specified, the meaning of "a plurality" is two or more; the orientation or positional relationship indicated by terms such as "upper", "lower", "left", "right", "inner", "outer", "front end", "rear end", "head", "tail", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be construed as a limitation to the present invention.

[0029] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principle and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A NK cell cryopreservation device, comprising a cryopreservation box (1) and a test tube (2), characterized in that: A plurality of placement plates (3) are provided in the freezing box (1) so as to slide horizontally from top to bottom. A plurality of placement grooves (31) for placing test tubes (2) are evenly provided on the placement plates (3). Arc-shaped clamping blocks (4) are provided on the left and right sides of the placement grooves (31) corresponding to the test tubes (2). Support blocks (5) are also provided at the bottom of the plurality of placement grooves (31). A support structure (6) is movably provided in the placement plates (3) corresponding to the plurality of support blocks (5). The support structure (6) is fixedly connected to the support blocks (5), and the support structure (6) is drivingly connected to the arc-shaped clamping blocks (4).

2. The NK cell cryopreservation device according to claim 1, characterized in that: The support structure (6) comprises a plurality of support rods (61), which are respectively arranged vertically on the lower side of the support block (5) and fixedly connected to the lower side of the support block (5); the plurality of support rods (61) are slidably connected to the placement plate (3); a movable cavity (32) is opened in the placement plate (3) at the bottom corresponding to the plurality of support rods (61); a movable plate (62) is transversely arranged in the movable cavity (32); the movable plate (62) is slidably connected to the movable cavity (32); the upper side of the movable plate (62) is fixedly connected to the bottom of the plurality of support rods (61); a threaded rod (63) is vertically arranged at the lower side of the placement plate (3) corresponding to the movable plate (62); the threaded rod (63) passes through the bottom of the placement plate (3) and extends into the movable plate (62) to be rotatably connected thereto; the threaded rod (63) is threadedly connected to the placement plate (3).

3. The NK cell cryopreservation device according to claim 2, characterized in that: An annular limiting block (64) is provided at one end of the threaded rod (63) located in the movable plate (62), and an annular limiting groove (65) is provided in the movable plate (62) corresponding to the annular limiting block (64), and the annular limiting block (64) is rotatably connected to the annular limiting groove (65).

4. The NK cell cryopreservation device according to claim 2, characterized in that: Abutment blocks (41) are respectively provided on the back side of the two arc-shaped clamping blocks (4); first movable grooves (33) are respectively provided in the placement plate (3) at the positions corresponding to the two abutment blocks (41); springs (42) are respectively transversely fixed on the back side of the two abutment blocks (41); the other end of the spring (42) abuts against the side wall of the first movable groove (33); a second movable groove (34) is provided on the upper side of the placement plate (3) corresponding to the support block (5); the lower ends of the two abutment blocks (41) abut against the left and right ends of the upper side surface of the support block (5); and the abutment surfaces of the abutment blocks (41) and the support block (5) are respectively provided with inclined surfaces (7) inclined outward from top to bottom.

5. The NK cell cryopreservation device according to claim 4, characterized in that: An arc-shaped positioning groove (51) is provided on the upper side of the support block (5) corresponding to the bottom of the test tube (2).

6. The NK cell cryopreservation device according to claim 1, characterized in that: The left and right side walls of the freezing box (1) are respectively provided with sliding grooves (11) in the horizontal and longitudinal directions corresponding to the plurality of placement plates (3), and the left and right sides of the placement plates (3) are respectively provided with limiting slide rails (35) corresponding to the sliding grooves (11).

7. The NK cell cryopreservation device according to claim 1, characterized in that: The front side of the placement plate (3) is fixed to the handle (36).

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