Equipment for separating and purifying nucleotide

Abstract

The utility model discloses equipment for separating and purifying nucleotide, and relates to the technical field of nucleotide processing. The device comprises a separation tank, a tank cover is detachably mounted at one end of the separation tank through a bolt, an equipment disc is fixedly mounted on the bottom wall of the separation tank, a connecting sleeve is arranged on the bottom wall of the separation tank, and a filter element is detachably mounted in the separation tank; the rotating separation mechanism drives the connecting sleeve to rotate, the filter element synchronously rotates along with the connecting sleeve, and the filter element filters and concentrates the nucleotide stock solution in the rotating process, so that the rotation of the filter element in the separation and purification process of the nucleotide stock solution is conveniently realized; the connecting sleeve is driven by the vibration mechanism to reciprocate up and down to generate vibration, and the filter element fixed in the connecting sleeve vibrates synchronously, so that the condition that filter holes and membrane holes in the filter element are blocked is avoided, and the separation and purification efficiency of the nucleotide stock solution is effectively improved. The separation and purification efficiency of the nucleotide stock solution is further improved.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleotide processing, in particular to a device for separating and purifying nucleotides. Background Art

[0002] Nucleotide is a class of compounds composed of purine base or pyrimidine base, ribose or deoxyribose and phosphate, also known as nucleotide acid;

[0003] When the nucleotide stock solution is separated and purified, it needs to be filtered and concentrated. However, the existing nucleotide separation and purification equipment still has some defects:

[0004] Existing nucleotide separation and purification equipment generally uses a filter element to filter and concentrate the nucleotide stock solution. However, since the filter element is mostly in a static state, the separation and purification efficiency of the nucleotide stock solution is low. At the same time, in the process of using the filter element to filter and concentrate the nucleotide stock solution, the filter pores and membrane pores in the filter element are prone to blockage, thereby affecting the separation and purification efficiency of the nucleotide stock solution. Utility Model Content

[0005] In order to solve the problem of low separation and purification efficiency of nucleotide stock solution, the utility model aims to provide a device for separation and purification of nucleotides.

[0006] In order to solve the above technical problems, the utility model adopts the following technical solutions: a nucleotide separation and purification device, comprising a separation tank, one end of which is detachably mounted with a tank cover by bolts, a device plate fixedly mounted on the bottom wall of the separation tank, a connecting sleeve provided on the bottom wall of the separation tank, a filter core detachably mounted inside the separation tank, one end of the filter core being arranged in the connecting sleeve, a rotating separation mechanism being arranged between the device plate and the connecting sleeve, and a vibration mechanism being arranged on the rotating separation mechanism and the tank cover;

[0007] The rotating separation mechanism includes a circular guide rail, which is fixedly mounted on the upper surface of the equipment disk, and two arc-shaped sliders are slidably mounted on the circular guide rail, and L-shaped connecting plates are fixedly mounted on the upper surfaces of the two arc-shaped connecting plates, and a first spring rod is fixedly mounted on one end of the two L-shaped connecting plates, and the telescopic ends of the two first spring rods are fixedly connected to the bottom end of the connecting sleeve, and a first rotating rod is rotatably mounted on the equipment disk, and a driving gear is fixedly mounted on one end of the first rotating rod, and an internal gear ring is fixedly mounted between the two arc-shaped sliders, and the driving gear and the internal gear ring are meshingly connected.

[0008] Preferably, an elastic ring is fixedly mounted on the inner wall of the connecting sleeve, and one end of the filter element is interference fit with the inner wall of the elastic ring.

[0009] Preferably, a feed bellows is fixedly installed on the other end of the filter core, and the feed bellows passes through the tank cover. A discharge bellows is fixedly installed on one end of the filter core, and the discharge bellows passes through the connecting sleeve, the equipment plate and the separation tank respectively.

[0010] Preferably, a motor is fixedly provided at the bottom end of the separation tank, and the other end of the first rotating rod is fixedly connected to the driving output end of the motor.

[0011] Preferably, the vibration mechanism includes two second rotating rods, one of which is rotatably mounted on an L-shaped connecting plate, one end of each of the two second rotating rods is fixedly mounted with a cam, and the bottom end of the connecting sleeve is fixedly mounted with two arcuate seats, one of which vertically corresponds to a cam.

[0012] Preferably, a transmission rod is rotatably installed on the two L-shaped connecting plates, a passive gear is fixedly installed on one end of the two transmission rods, a toothed disc is fixedly installed on the upper surface of the equipment disk, the two passive gears and the toothed disc are meshingly connected, a synchronous wheel is fixedly installed on one end of the two transmission rods and the other end of the two second rotating rods, wherein a synchronous belt is transmission-installed between two adjacent synchronous wheels, two second spring rods are fixedly installed on the top wall of the tank cover, and limit disks are fixedly installed on the telescopic ends of the two second spring rods.

[0013] Compared with the prior art, the beneficial effects of the utility model are:

[0014] 1. In the utility model, the connection sleeve is driven to rotate by the rotary separation mechanism, and the filter core rotates synchronously with the connection sleeve. The filter core filters and concentrates the nucleotide stock solution during the rotation process, thereby conveniently realizing the rotation of the filter core during the separation and purification process of the nucleotide stock solution, thereby effectively improving the efficiency of the separation and purification of the nucleotide stock solution;

[0015] 2. In the utility model, the vibration mechanism drives the connecting sleeve to reciprocate up and down to generate vibration, and the filter element fixed in the connecting sleeve vibrates synchronously, thereby avoiding the clogging of the filter holes and membrane holes in the filter element, and further improving the efficiency of separation and purification of nucleotide stock solution. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0017] Figure 1The utility model is a schematic diagram of the overall structure of a device for separating and purifying nucleotides.

[0018] Figure 2 It is a schematic diagram of the cutaway structure of the separation tank and the tank cover of the utility model.

[0019] Figure 3 It is a schematic diagram of the separation structure of the filter element and the connecting sleeve of the utility model.

[0020] Figure 4 For this utility model Figure 3 A magnified schematic diagram of part A in FIG.

[0021] Figure 5 It is a schematic diagram of the connection between the rotary separation mechanism, the vibration mechanism, the equipment disk and the connecting sleeve of the utility model.

[0022] Figure 6 For this utility model Figure 5 An enlarged schematic diagram of part B in FIG.

[0023] In the figure: 1. separation tank; 11. tank cover; 12. equipment plate; 13. connecting sleeve; 14. elastic ring; 2. filter element; 21. feed bellows; 22. discharge bellows; 3. rotary separation mechanism; 31. circular guide rail; 32. arc slider; 33. L-shaped connecting plate; 34. first spring rod; 35. first rotating rod; 36. driving gear; 37. inner gear ring; 38. motor; 39. L-shaped support frame; 4. vibration mechanism; 41. second rotating rod; 42. cam; 43. arc seat; 44. transmission rod; 45. passive gear; 46. toothed disc; 47. synchronous wheel; 48. synchronous belt; 49. second spring rod; 5. limit plate; 51. anti-skid pad. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0025] Example: Figure 1-6As shown, the utility model provides a device for separating and purifying nucleotides, including a separation tank 1, one end of the separation tank 1 is detachably mounted with a tank cover 11 by bolts, a device disk 12 is fixedly mounted on the bottom wall of the separation tank 1, a connecting sleeve 13 is provided on the bottom wall of the separation tank 1, a filter core 2 is detachably mounted inside the separation tank 1, the filter core 2 is composed of a nano-membrane and a filter screen, and can filter and concentrate a nucleotide stock solution to achieve separation and purification of the nucleotide stock solution, which is a prior art and will not be described in detail herein, one end of the filter core 2 is arranged in the connecting sleeve 13, a rotating separation mechanism 3 is arranged between the device disk 12 and the connecting sleeve 13, by arranging the rotating separation mechanism 3, the rotating separation mechanism 3 can rotate the filter core 2, thereby improving the efficiency of separation and purification of the nucleotide stock solution, a vibration mechanism 4 is arranged on the rotating separation mechanism 3 and the tank cover 11, by arranging the vibration mechanism 4, the vibration mechanism 4 can make the filter core 2 vibrate up and down, thereby avoiding the blockage of the filter holes and membrane holes in the filter core 2, and further improving the efficiency of separation and purification of the nucleotide stock solution;

[0026] The rotating separation mechanism 3 includes a circular guide rail 31, which is fixedly mounted on the upper surface of the device disk 12. Two arc-shaped sliders 32 are slidably mounted on the circular guide rail 31. L-shaped connecting plates 33 are fixedly mounted on the upper surfaces of the two arc-shaped sliders 32. One end of the two L-shaped connecting plates 33 is fixedly mounted with a first spring rod 34. The telescopic ends of the two first spring rods 34 are fixedly connected to the bottom end of the connecting sleeve 13. A first rotating rod 35 is rotatably mounted on the device disk 12. A driving gear 36 is fixedly mounted on one end of the first rotating rod 35. An inner gear ring 37 is fixedly installed between the two arc-shaped sliders 32, and the driving gear 36 is meshingly connected with the inner gear ring 37. By driving the arc-shaped slider 32 to slide in a circle on the circular guide rail 31, the arc-shaped slider 32 can rotate the filter element 2 through the L-shaped connecting plate 33, the first spring rod 34 and the connecting sleeve 13. By driving the first rotating rod 35 to rotate, the first rotating rod 35 can rotate the driving gear 36, and the driving gear 36 can drive the inner gear ring 37 to rotate. The inner gear ring 37 can make the arc-shaped slider 32 slide in a circle on the circular guide rail 31.

[0027] An elastic ring 14 is fixedly installed on the inner wall of the connecting sleeve 13, and one end of the filter element 2 is interference fit with the inner wall of the elastic ring 14. By arranging the elastic ring 14 in the connecting sleeve 13, when the tank cover 11 is removed, the filter element 2 can be vertically placed in the separation tank 1, and the bottom end of the filter element 2 enters the connecting sleeve 13 and squeezes the elastic ring 14. The elastic ring 14 shrinks and fixes the filter element 2 by interference fit, thereby facilitating the disassembly of the filter element 2 and facilitating the replacement of the filter element 2.

[0028] A feed bellows 21 is fixedly installed on the other end of the filter core 2, and the feed bellows 21 passes through the tank cover 11. A discharge bellows 22 is fixedly installed on one end of the filter core 2, and the discharge bellows 22 respectively pass through the connecting sleeve 13, the equipment plate 12 and the separation tank 1. By arranging the feed bellows 21 and the discharge bellows 22, the nucleotide stock solution to be separated and purified can enter the filter core 2 through the feed bellows 21, and the extract filtered and concentrated by the filter core 2 can be discharged through the discharge bellows 22.

[0029] A motor 38 is fixedly provided at the bottom end of the separation tank 1 , and the other end of the first rotating rod 35 is fixedly connected to the driving output end of the motor 38 . By turning on the motor 38 , the driving shaft of the motor 38 can rotate the first rotating rod 35 .

[0030] An L-shaped support frame 39 is fixedly installed at the fixed end of the motor 38 , and one end of the L-shaped support frame 39 is fixedly connected to the bottom end of the separation tank 1 . By setting the L-shaped support frame 39 , the L-shaped support frame 39 can support and fix the motor 38 .

[0031] The vibration mechanism 4 includes two second rotating rods 41, one of which is rotatably mounted on an L-shaped connecting plate 33, and one end of the two second rotating rods 41 is fixedly mounted with a cam 42. The bottom end of the connecting sleeve 13 is fixedly mounted with two arc seats 43, and one arc seat 43 vertically corresponds to a cam 42. By driving the second rotating rod 41 to rotate, the second rotating rod 41 can rotate the cam 42, and the cam 42 can make the connecting sleeve 13 and the filter element 2 reciprocate downward to generate vibration through cooperation with the arc seat 43 and the action of the first spring rod 34.

[0032] A transmission rod 44 is rotatably mounted on the two L-shaped connecting plates 33, and a passive gear 45 is fixedly mounted on one end of the two transmission rods 44. A toothed disc 46 is fixedly mounted on the upper surface of the equipment disk 12. The two passive gears 45 and the toothed disc 46 are meshed and connected. A synchronous wheel 47 is fixedly mounted on one end of the two transmission rods 44 and the other end of the two second rotating rods 41, wherein a synchronous belt 48 is installed between two adjacent synchronous wheels 47. Two second spring rods 49 are fixedly mounted on the top wall of the tank cover 11, and the telescopic ends of the two second spring rods 49 are fixedly mounted. A limit plate 5 is installed. When the connecting sleeve 13 rotates, the transmission rod 44 and the driven gear 45 revolve in a circle. The toothed plate 46 forces the driven gear 45 to rotate. The driven gear 45 causes the transmission rod 44 to rotate. The transmission rod 44 can cause the second rotating rod 41 to rotate through the synchronous wheel 47 and the synchronous belt 48. By arranging the second spring rod 49 and the limit plate 5, when the filter element 2 rises, the filter element 2 contracts the telescopic end of the second spring rod 49 through the limit plate 5. During the descent of the filter element 2, the telescopic end of the second spring rod 49 extends, causing the limit plate 5 to follow the descent.

[0033] The lower surfaces of the two limit plates 5 are fixedly mounted with anti-skid pads 51, and the lower surfaces of the two anti-skid pads 51 are in movable contact with the other end of the filter element 2. By providing the anti-skid pads 51, the anti-skid pads 51 can improve the stability between the limit plates 5 and the filter element 2.

[0034] Working principle: When the nucleotide stock solution needs to be separated and purified, the operator first introduces the nucleotide stock solution into the filter element 2 through the feed bellows 21. The filter screen and nano-membrane in the filter element 2 filter and concentrate the nucleotide stock solution. The extract obtained by filtration and concentration is discharged through the discharge bellows 22.

[0035] During this process, the operator turns on the motor 38, the driving shaft of the motor 38 rotates the first rotating rod 35, the first rotating rod 35 rotates the driving gear 36, the driving gear 36 drives the inner gear ring 37 to rotate, the inner gear ring 37 causes the two arc-shaped sliders 32 to slide circumferentially on the circular guide rail 31, and the two arc-shaped sliders 32 rotate the filter element 2 through the two L-shaped connecting plates 33, the two first spring rods 34 and the connecting sleeve 13, so that the filter element 2 filters and concentrates the nucleotide stock solution during the rotation process, thereby conveniently realizing the rotation of the filter element 2 during the separation and purification process of the nucleotide stock solution, thereby effectively improving the efficiency of the separation and purification of the nucleotide stock solution;

[0036] At the same time, the two L-shaped connecting plates 33 cause the two transmission rods 44 and the two passive gears 45 to revolve in a circle, the toothed disc 46 forces the two passive gears 45 to rotate, the two passive gears 45 cause the two transmission rods 44 to rotate, the two transmission rods 44 cause the two second rotating rods 41 to rotate through the corresponding two synchronous wheels 47 and a synchronous belt 48, the two second rotating rods 41 cause the two cams 42 to rotate, and the two cams 42 cause the connecting sleeve 13 and the filter element 2 to reciprocate up and down through the two arc seats 43 to generate vibration, thereby avoiding the clogging of the filter holes and membrane holes in the filter element 2, and further improving the efficiency of separation and purification of the nucleotide stock solution.

[0037] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A device for separating and purifying nucleotides, comprising a separation tank (1), characterized in that: A tank cover (11) is detachably mounted on one end of the separation tank (1) by means of bolts, a device plate (12) is fixedly mounted on the bottom wall of the separation tank (1), a connecting sleeve (13) is provided on the bottom wall of the separation tank (1), a filter core (2) is detachably mounted inside the separation tank (1), one end of the filter core (2) is arranged in the connecting sleeve (13), a rotating separation mechanism (3) is provided between the device plate (12) and the connecting sleeve (13), and a vibration mechanism (4) is provided on the rotating separation mechanism (3) and the tank cover (11); The rotating separation mechanism (3) comprises a circular guide rail (31), the circular guide rail (31) is fixedly mounted on the upper surface of the equipment disk (12), two arc-shaped sliders (32) are slidably mounted on the circular guide rail (31), an L-shaped connecting plate (33) is fixedly mounted on the upper surface of the two arc-shaped sliders (32), one end of the two L-shaped connecting plates (33) is fixedly mounted with a first spring rod (34), the telescopic ends of the two first spring rods (34) are fixedly connected to the bottom end of the connecting sleeve (13), a first rotating rod (35) is rotatably mounted on the equipment disk (12), one end of the first rotating rod (35) is fixedly mounted with a driving gear (36), an inner gear ring (37) is fixedly mounted between the two arc-shaped sliders (32), and the driving gear (36) and the inner gear ring (37) are meshingly connected.

2. A nucleotide separation and purification device as claimed in claim 1, characterized in that: An elastic ring (14) is fixedly mounted on the inner wall of the connecting sleeve (13), and one end of the filter core (2) is interference-fitted with the inner wall of the elastic ring (14).

3. A nucleotide separation and purification device as claimed in claim 1, characterized in that: A feed bellows (21) is fixedly mounted on the other end of the filter core (2), and the feed bellows (21) passes through the tank cover (11). A discharge bellows (22) is fixedly mounted on one end of the filter core (2), and the discharge bellows (22) passes through the connecting sleeve (13), the equipment plate (12), and the separation tank (1), respectively.

4. The device for separating and purifying nucleotides according to claim 1, characterized in that: A motor (38) is fixedly provided at the bottom end of the separation tank (1), and the other end of the first rotating rod (35) is fixedly connected to the driving output end of the motor (38).

5. A device for separating and purifying nucleotides as claimed in claim 4, characterized in that: An L-shaped support frame (39) is fixedly mounted on the fixed end of the motor (38), and one end of the L-shaped support frame (39) is fixedly connected to the bottom end of the separation tank (1).

6. The device for separating and purifying nucleotides according to claim 1, characterized in that: The vibration mechanism (4) comprises two second rotating rods (41), one of which is rotatably mounted on an L-shaped connecting plate (33), one end of each of the two second rotating rods (41) is fixedly mounted with a cam (42), and the bottom end of each of the connecting sleeves (13) is fixedly mounted with two arc seats (43), one of which vertically corresponds to a cam (42).

7. The device for separating and purifying nucleotides according to claim 6, characterized in that: A transmission rod (44) is rotatably mounted on the two L-shaped connecting plates (33), and a passive gear (45) is fixedly mounted on one end of the two transmission rods (44). A toothed disc (46) is fixedly mounted on the upper surface of the equipment disk (12), and the two passive gears (45) and the toothed disc (46) are meshingly connected. A synchronous wheel (47) is fixedly mounted on one end of the two transmission rods (44) and the other end of the two second rotating rods (41), wherein a synchronous belt (48) is transmission-mounted between two adjacent synchronous wheels (47). Two second spring rods (49) are fixedly mounted on the top wall of the tank cover (11), and a limit disc (5) is fixedly mounted on the telescopic ends of the two second spring rods (49).

8. The device for separating and purifying nucleotides according to claim 7, characterized in that: Anti-skid pads (51) are fixedly mounted on the lower surfaces of the two limiting plates (5), and the lower surfaces of the two anti-skid pads (51) are in movable contact with the other end of the filter element (2).

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