Plasma separation and rapid detection integrated device

Abstract

The utility model discloses a plasma separation and rapid detection integrated device which comprises a storage tank, a limiting ring is fixedly installed on the inner side of the upper end of the storage tank, a containing tank is installed on the inner side of the limiting ring, a heating block is installed at the bottom end of the containing tank, a plurality of EP pipes are installed on the inner side of the containing tank, and a heating block is installed at the bottom end of the containing tank. A sealing film is arranged on the upper end face of the EP pipe. A connecting sleeve is mounted on the outer side of the upper end of the storage tank, a pressurizing cover is mounted on the inner side of the upper end of the connecting sleeve, an elastic connecting belt is arranged on one side of the connecting sleeve, a filtering mounting sleeve is mounted on the lower end face of the pressurizing cover, and a double-layer plasma separation filter membrane is mounted on the inner side of the bottom end of the filtering mounting sleeve. Collected blood is immediately pressurized and filtered, and meanwhile, the EP tube filled with a plasma rapid detection reagent can be used for constant-temperature storage, so that blood sample pollution, hemolysis possibly caused in the normal-temperature transportation process of a whole blood sample and the like are effectively avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of blood separation, in particular to a plasma separation and rapid detection integrated device. Background Art

[0002] Plasma is a component of human blood, which contains many important biomolecules, such as proteins, hormones and drugs. By separating plasma, we can better understand the health of the human body, and it can also be used for clinical diagnosis and treatment. However, traditional separation methods are often complicated, time-consuming, and require a large amount of blood samples. The existing technology usually draws blood from the vein, mixes the plasma and serum in the blood, and then separates the plasma and serum by centrifugation.

[0003] The existing centrifugal method for separating blood is complicated to operate, time-consuming, and easily causes hemolysis during transportation at room temperature; therefore, it does not meet existing needs. In this regard, we have proposed an integrated device for plasma separation and rapid detection. Utility Model Content

[0004] The purpose of the utility model is to provide an integrated device for plasma separation and rapid detection, so as to solve the problems of complicated operation, long time consumption and easy hemolysis caused by normal temperature transportation when separating blood by the existing centrifugal method proposed in the above background technology.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a plasma separation and rapid detection integrated device, comprising a storage tank, a limiting ring is fixedly installed on the inner side of the upper end of the storage tank, a placement tank is installed on the inner side of the limiting ring, a heating block is installed on the bottom end of the placement tank, a plurality of EP tubes are installed on the inner side of the placement tank, and a sealing film is provided on the upper end surface of the EP tube;

[0006] A connecting sleeve is installed on the outer side of the upper end of the preservation tank, a pressure cover is installed on the inner side of the upper end of the connecting sleeve, an elastic connecting belt is provided on one side of the connecting sleeve, a filter mounting sleeve is installed on the lower end surface of the pressure cover, and a double-layer plasma separation filter membrane is installed on the inner side of the bottom end of the filter mounting sleeve.

[0007] Preferably, a supporting bottom cover is installed on the inner side of the bottom end of the preservation tank, a battery block is installed on the upper end surface of the supporting bottom cover, and a thermal insulation sheet is installed on the upper end surface of the battery block.

[0008] Preferably, a puncture guide seat is fixedly installed at the bottom end of the filter mounting sleeve, and a plurality of conical hollow puncture heads are provided on the lower end surface of the puncture guide seat. The puncture guide seat and the plurality of conical hollow puncture heads are integrally injection molded, and a guide hole is provided on the inner side of the conical hollow puncture head.

[0009] Preferably, the bottom end of the preservation tank is connected to the supporting bottom cover by threads, the middle part of the supporting bottom cover passes through the battery block, the thermal insulation sheet and the heating block and is in close contact with the lower end surface of the placement tank, the heating block is connected to the battery block by the thermal insulation sheet, the heating block is electrically connected to the battery block, and an electric thermal resistance wire is provided inside the heating block.

[0010] Preferably, both ends of the elastic connecting band are fixedly connected to the connecting sleeve and the pressure cover, the pressure cover and the filter mounting sleeve are slidably connected to the connecting sleeve, and the filter mounting sleeve is snap-fitted and mounted to the double-layer plasma separation filter membrane.

[0011] Preferably, the EP tube is bonded and fixed to a sealing film, the sealing film is tin foil, and the interior of the EP tube is filled with a rapid plasma detection reagent.

[0012] Compared with the prior art, the beneficial effects of the utility model are:

[0013] 1. The utility model locks and installs the placement tank by supporting the bottom cover and the limit ring, and uses a pipette to absorb the collected human venous blood to the inner side of the filter installation sleeve, that is, the upper end surface of the double-layer plasma separation filter membrane, and inserts the pressure cover to the inner side of the connecting sleeve and presses the filter installation sleeve, so that the filter installation sleeve drives the puncture guide seat and the double-layer plasma separation filter membrane to move downward synchronously, and then the puncture guide seat punctures the closed membrane through the conical hollow puncture head, realizing the through connection between the conical hollow puncture head and the EP tube, and then the venous blood is filtered through the double-layer plasma separation filter membrane and the conical hollow puncture head;

[0014] 2. The utility model installs a plasma rapid detection reagent on the inner side of the EP tube, and heats the plasma rapid detection reagent at a constant temperature through a heating block via a placement tank and the EP tube, thereby separating plasma nucleic acids in time and retaining larger DNA molecules, effectively avoiding hemolysis and nucleic acid degradation that may occur during the normal temperature transportation of whole blood samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0016] Figure 2 It is a schematic diagram of the cross-sectional structure of the utility model as a whole;

[0017] Figure 3 This is a schematic diagram of the structure of the placement tank of the utility model;

[0018] Figure 4 It is a structural schematic diagram of the EP pipe of the utility model.

[0019] In the figure: 1. Preservation tank; 2. Connecting sleeve; 3. Pressure cover; 4. Elastic connecting belt; 5. Support bottom cover; 6. Battery block; 7. Insulation sheet; 8. Heating block; 9. Placement tank; 10. Limiting ring; 11. EP tube; 12. Sealing membrane; 13. Filter installation sleeve; 14. Puncture guide seat; 15. Double-layer plasma separation filter membrane; 16. Conical hollow puncture head. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0021] See also Figures 2 to 4 The utility model provides an embodiment: a plasma separation and rapid detection integrated device, comprising a preservation tank 1, a limiting ring 10 is fixedly installed on the inner side of the upper end of the preservation tank 1, a placement tank 9 is installed on the inner side of the limiting ring 10, a heating block 8 is installed on the bottom end of the placement tank 9, a plurality of EP tubes 11 are installed on the inner side of the placement tank 9, a closing film 12 is provided on the upper end surface of the EP tube 11, the EP tube 11 is bonded and fixed to the closing film 12, the closing film 12 is tin foil, the interior of the EP tube 11 is filled with a plasma rapid detection reagent, and the closing film 12 can keep the internal plasma rapid detection reagent stable when the EP tube 11 is not in use.

[0022] See also Figure 1 and Figure 2 A connecting sleeve 2 is installed on the outer side of the upper end of the preservation tank 1, a pressure cover 3 is installed on the inner side of the upper end of the connecting sleeve 2, an elastic connecting belt 4 is provided on one side of the connecting sleeve 2, a filter mounting sleeve 13 is installed on the lower end surface of the pressure cover 3, and a double-layer plasma separation filter membrane 15 is installed on the inner side of the bottom end of the filter mounting sleeve 13, and blood can be filtered through the double-layer plasma separation filter membrane 15.

[0023] See also Figures 1 to 4 A puncture guide seat 14 is fixedly installed at the bottom end of the filter installation sleeve 13, and a plurality of conical hollow puncture heads 16 are provided on the lower end surface of the puncture guide seat 14. The puncture guide seat 14 and the plurality of conical hollow puncture heads 16 are integrally injection molded, and a guide hole is provided on the inner side of the conical hollow puncture head 16. Both ends of the elastic connecting belt 4 are fixedly connected to the connecting sleeve 2 and the pressure cover 3, and the pressure cover 3 and the filter installation sleeve 13 are both slidably connected to the connecting sleeve 2. The filter installation sleeve 13 is clamped and installed with the double-layer plasma separation filter membrane 15. By pressing the pressure cover 3, the conical hollow puncture head 16 can puncture the closed membrane 12, thereby facilitating the penetration of the conical hollow puncture head 16 and the EP tube 11, thereby realizing the diversion of blood.

[0024] See also Figure 2A supporting bottom cover 5 is installed on the inner side of the bottom end of the storage tank 1, a battery block 6 is installed on the upper end surface of the supporting bottom cover 5, and a thermal insulation sheet 7 is installed on the upper end surface of the battery block 6. The thermal insulation sheet 7 can effectively insulate the heating block 8 and prevent the battery block 6 from being damaged by heat;

[0025] The bottom end of the preservation tank 1 is connected to the supporting bottom cover 5 by threads, the middle part of the supporting bottom cover 5 passes through the battery block 6, the thermal insulation sheet 7 and the heating block 8 and is in contact with the lower end surface of the placement tank 9, the heating block 8 is connected to the battery block 6 by the thermal insulation sheet 7, the heating block 8 is electrically connected to the battery block 6, and an electric thermal resistance wire is provided inside the heating block 8. The plasma rapid detection reagent is heated at a constant temperature through the heating block 8, the placement tank 9 and the EP tube 11.

[0026] When in use, the placement tank 9 is plugged into the inner side of the storage tank 1, and the battery block 6, the thermal insulation sheet 7 and the heating block 8 are sequentially placed on the upper end surface of the support bottom cover 5, so that the middle part of the support bottom cover 5 passes through the battery block 6, the thermal insulation sheet 7 and the heating block 8 and is connected to the storage tank 1 through threads, so that the support bottom cover 5 and the limiting ring 10 lock and install the placement tank 9;

[0027] Turn on the power supply, place a plurality of EP tubes 11 bonded with sealing films 12 on the inner side of the placement tank 9, so that when the connection sleeve 2 is threadedly connected to the upper end of the storage tank 1, the upper end of the placement tank 9 is sealed through the filter installation sleeve 13 and the puncture guide seat 14, and the collected human venous blood is sucked to the inner side of the filter installation sleeve 13, i.e., the upper end surface of the double-layer plasma separation filter membrane 15, by using a pipette gun. After the static state is completed, the pressure cover 3 is inserted into the inner side of the connection sleeve 2 and the filter installation sleeve 13 is pressed;

[0028] The filter installation sleeve 13 drives the puncture guide seat 14 and the double-layer plasma separation filter membrane 15 to move downward synchronously, and then the puncture guide seat 14 punctures the closed membrane 12 through the conical hollow puncture head 16, so as to realize the through connection between the conical hollow puncture head 16 and the EP tube 11, and then the venous blood can be filtered by the double-layer plasma separation filter membrane 15 and guided by the conical hollow puncture head 16 to the inner side of multiple EP tubes 11. The inner side of the EP tube 11 is equipped with a plasma rapid detection reagent. The plasma rapid detection reagent is heated at a constant temperature by the heating block 8 through the placement tank 9 and the EP tube 11, so that the plasma / serum can be separated in time, and the hemolysis and nucleic acid degradation that may be caused during the room temperature transportation of the whole blood sample are effectively avoided.

[0029] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.

Claims

1. A plasma separation and rapid detection integrated device, comprising a storage tank (1), characterized in that: A limiting ring (10) is fixedly installed on the inner side of the upper end of the storage tank (1), a placement tank (9) is installed on the inner side of the limiting ring (10), a heating block (8) is installed on the bottom end of the placement tank (9), a plurality of EP tubes (11) are installed on the inner side of the placement tank (9), and a sealing film (12) is provided on the upper end surface of the EP tube (11); A connecting sleeve (2) is installed on the outer side of the upper end of the storage tank (1), a pressure cover (3) is installed on the inner side of the upper end of the connecting sleeve (2), an elastic connecting belt (4) is provided on one side of the connecting sleeve (2), a filter mounting sleeve (13) is installed on the lower end surface of the pressure cover (3), and a double-layer plasma separation filter membrane (15) is installed on the inner side of the bottom end of the filter mounting sleeve (13).

2. The plasma separation and rapid detection integrated device according to claim 1, characterized in that: A supporting bottom cover (5) is installed on the inner side of the bottom end of the storage tank (1), a battery block (6) is installed on the upper end surface of the supporting bottom cover (5), and a thermal insulation sheet (7) is installed on the upper end surface of the battery block (6).

3. The plasma separation and rapid detection integrated device according to claim 1, characterized in that: A puncture guide seat (14) is fixedly mounted on the bottom end of the filter mounting sleeve (13); a plurality of conical hollow puncture heads (16) are arranged on the lower end surface of the puncture guide seat (14); the puncture guide seat (14) and the plurality of conical hollow puncture heads (16) are integrally injection molded; and a guide hole is arranged on the inner side of the conical hollow puncture head (16).

4. The plasma separation and rapid detection integrated device according to claim 2, characterized in that: The bottom end of the storage tank (1) is connected to the supporting bottom cover (5) by means of threads, the middle part of the supporting bottom cover (5) penetrates the battery block (6), the thermal insulation sheet (7) and the heating block (8) and is in close contact with the lower end surface of the placement tank (9), the heating block (8) is connected to the battery block (6) by means of the thermal insulation sheet (7), the heating block (8) is electrically connected to the battery block (6), and an electric heat resistance wire is provided inside the heating block (8).

5. The plasma separation and rapid detection integrated device according to claim 1, characterized in that: Both ends of the elastic connecting band (4) are fixedly connected to the connecting sleeve (2) and the pressure cover (3); the pressure cover (3) and the filter mounting sleeve (13) are slidably connected to the connecting sleeve (2); and the filter mounting sleeve (13) is snap-fitted and mounted to the double-layer plasma separation filter membrane (15).

6. The plasma separation and rapid detection integrated device according to claim 1, characterized in that: The EP tube (11) is bonded and fixed to a sealing film (12), the sealing film (12) is tin foil, and the interior of the EP tube (11) is filled with a rapid blood plasma detection reagent.

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