Lateral detection mechanism and optical detection device

Abstract

The utility model discloses a lateral detection mechanism and an optical detection device. The lateral detection mechanism comprises a fluorescence detection module; the lateral coupling module and the fluorescence detection module are arranged at intervals, and the lateral coupling module comprises a lateral coupling support, a lateral coupling module, a lateral coupling module and a lateral coupling module; the signal collection assembly comprises a coupling mirror module used for coupling lateral scattering signals, and the signal collection assembly is connected with the lateral coupling support; one end of the optical fiber assembly is connected with the signal collection assembly, the other end of the optical fiber assembly is connected with the fluorescence detection module, and the coupled lateral scattering signals are transmitted to the fluorescence detection module so as to collect and analyze the lateral scattering signals. Wherein the signal collection assembly and the fluorescence detection module are connected through the optical fiber assembly, the relative position of the fluorescence detection module and the signal collection assembly can be adjusted, and the installation flexibility of the fluorescence detection module is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell analysis equipment, in particular to a lateral detection mechanism, an optical detection device, etc. Background Art

[0002] In the laser system of a flow cytometer, the laser irradiates a sample labeled with fluorescence passing through a flow cell. The flow cell is an optical compound microscope objective lens, which can focus the forward beam on the sample labeled with fluorescein flowing through its center. After the fluorescein is excited, it will generate scattered fluorescence and pass through the optical lens group on its side, and finally the fluorescence detection module collects the signal.

[0003] For the lateral scattered fluorescence signal, due to its weak intensity itself, currently a PMT detector with strong signal amplification ability and high sensitivity is mostly used for analysis. In order to obtain the maximum lateral scattered fluorescence signal, it is necessary to transmit it to the fluorescence detection module through a certain coupling device, and through the corresponding optical system, transmit these signals to the target surface of the PMT detector to realize the collection and analysis of the signals.

[0004] In the related art, the relative position between the fluorescence collection module and the lateral mirror group of the flow cell is fixed. In this type of flow cytometer using a PMT detector, usually two lasers are used, and the coupling device for the lateral fluorescence signal realizes the adjustment in two dimensions of front-back and left-right through an adjustable structure device. This method has low coupling efficiency due to fewer adjustment dimensions. Summary of the Utility Model

[0005] The utility model aims to solve at least one of the technical problems existing in the prior art. For this purpose, the utility model provides a lateral detection mechanism, in which the signal collection component and the fluorescence detection module are connected through an optical fiber component, and the relative position of the fluorescence detection module and the signal collection component can be adjusted, improving the flexibility of the installation of the fluorescence detection module.

[0006] The utility model further provides an optical detection device.

[0007] The lateral detection mechanism according to the first aspect embodiment of the utility model includes: a fluorescence detection module; a lateral coupling module, the lateral coupling module and the fluorescence detection module are arranged at intervals from each other, the lateral coupling module includes: a lateral coupling bracket; a signal collection component, including: a coupling mirror module for coupling the lateral scattered signal, the signal collection component is connected to the lateral coupling bracket; an optical fiber component, one end of the optical fiber component is connected to the signal collection component and the other end is connected to the fluorescence detection module, and transmits the coupled lateral scattered signal to the fluorescence detection module to collect and analyze the lateral scattered signal.

[0008] According to the lateral detection mechanism of the embodiments of the present utility model, the signal collection component and the fluorescence detection module are connected through an optical fiber component, and the relative positions of the fluorescence detection module and the signal collection component can be adjusted, improving the flexibility of the installation of the fluorescence detection module.

[0009] According to some embodiments of the present utility model, the lateral coupling module further includes: an adjustment component, which is arranged on the lateral coupling bracket, and the adjustment component can make the signal collection component rotate around the axis of the coupling mirror module; and / or, the adjustment component can make the signal collection component move along the axis of the coupling mirror module, and / or, the adjustment component can make the signal collection component move in the height direction; and / or, the adjustment component can make the signal collection component move in the first direction.

[0010] According to some embodiments of the present utility model, the signal collection component includes: an optical fiber connection part, which is connected to the lateral coupling bracket, and the optical fiber connection part connects the coupling mirror module and the optical fiber component.

[0011] According to some embodiments of the present utility model, the optical fiber component includes: a first connection part and a plurality of optical fibers, one end of the first connection part is connected to the optical fiber connection part and the other end is connected to one ends of the plurality of optical fibers, and the other ends of the plurality of optical fibers are connected to the fluorescence detection component.

[0012] According to some embodiments of the present utility model, the first connection part is provided with a plurality of first light collection holes arranged vertically, and the plurality of first light collection holes are used for transmitting the lateral scattering signal from the optical fiber connection part to the plurality of optical fibers.

[0013] According to some embodiments of the present utility model, the fluorescence detection module includes: a mounting bracket and a collimating mirror module, the collimating mirror module is fixedly connected to the mounting bracket, the other end of the optical fiber component is connected to the collimating mirror module, and the collimating mirror module includes: a collimating mirror bracket and a plurality of collimating mirrors, and the plurality of collimating mirrors are mounted on the collimating mirror bracket.

[0014] According to some embodiments of the present utility model, the fluorescence detection module further includes: a plurality of lens integrated brackets, the plurality of lens integrated brackets are fixedly connected to the mounting bracket, the lens integrated brackets are provided with a plurality of first mounting grooves inclined in a second direction, the first mounting grooves are used for mounting dichroic mirrors or reflecting mirrors, and the directions of at least two of the first mounting grooves are different from the directions of at least another of the first mounting grooves; the lens integrated brackets are further provided with second mounting grooves spaced from the first mounting grooves for mounting focusing lenses.

[0015] According to some embodiments of the present utility model, the fluorescence detection module further includes: a plurality of detector modules, the plurality of detector modules are fixedly connected to the mounting bracket and respectively correspond to a plurality of collimators, the plurality of detector modules are disposed opposite to each other on both sides of the base, and at least two of the detector modules are disposed on the same side.

[0016] An optical detection device according to an embodiment of the second aspect of the present utility model includes: a flow cell for allowing a sample to be measured to pass through and providing a place for the sample to be irradiated by a light source; a light source for emitting light and incident on the sample to be measured flowing through the flow cell; and the lateral detection mechanism for collecting lateral scattering signals generated when the light source irradiates the sample to be measured.

[0017] According to some embodiments of the present utility model, the optical detection device further includes: a sampling device communicating with the flow cell for sucking the sample to be measured from a sample tube and transporting it to the flow cell.

[0018] Additional aspects and advantages of the present utility model will be given in part in the following description, become apparent in part from the following description, or be learned through the practice of the present utility model. Description of the Drawings

[0019] The above and / or additional aspects and advantages of the present utility model will become apparent and be readily understood from the description of the embodiments in conjunction with the following drawings, wherein:

[0020] Figure 1 is an overall schematic diagram of a lateral detection mechanism according to an embodiment of the present utility model;

[0021] Figure 2 is a structural schematic diagram of a lateral coupling module and an optical fiber assembly according to an embodiment of the present utility model;

[0022] Figure 3 is a structural schematic diagram of a fluorescence detection module according to an embodiment of the present utility model;

[0023] Figure 4 is an exploded view of a lateral detection mechanism according to an embodiment of the present utility model;

[0024] Figure 5 is a structural schematic diagram of a lens integrated bracket according to an embodiment of the present utility model.

[0025] Reference Numerals:

[0026] 100, lateral detection mechanism;

[0027] 10. Fluorescence detection module; 11. Mounting bracket; 12. Collimator module; 13. Lens integrated bracket; 131. First mounting groove; 132. Second mounting groove; 14. Detector module; 141. Detector; 15. Filter assembly;

[0028] 20. Lateral coupling module; 21. Lateral coupling bracket; 22. Signal collection assembly; 221. Fiber optic connector; 222. Second interface flange; 23. First adjustment assembly; 24. Third adjustment assembly; 25. Fourth adjustment assembly;

[0029] 30. Fiber optic assembly; 31. First connector; 311. First light receiving hole; 32. Optical fiber; 33. Second connector;

[0030] 41. Dichroic mirror; 42. Focusing lens; 43. Filter; 44. Reflecting mirror. Detailed implementation manners

[0031] The embodiments of the present invention will be described in detail below. The embodiments described with reference to the drawings are exemplary. The embodiments of the present invention will be described in detail below.

[0032] Below, reference is made to Figures 1 - 5 Describe the lateral detection mechanism 100 according to the embodiments of the present invention, and also propose an optical detection device including the above-mentioned lateral detection mechanism 100.

[0033] As Figure 1 and Figure 4 shown, the lateral detection mechanism 100 includes: a fluorescence detection module 10 and a lateral coupling module 20. The lateral coupling module 20 and the fluorescence detection module 10 are arranged at intervals. Specifically, after the light beam irradiates the sample to be measured, a lateral scattering signal is generated. In order to obtain the maximum lateral scattering signal, a lateral coupling module 20 is arranged on the side of the sample to be measured. The lateral coupling module 20 can collect the lateral scattering signal on the side of the sample to be measured, and finally the fluorescence detection module 10 collects and analyzes the lateral scattering signal.

[0034] As Figure 2 shown, the lateral coupling module 20 includes: a lateral coupling bracket 21, a signal collection assembly 22 and a fiber optic assembly 30. The lateral coupling bracket 21 is used to mount the signal collection assembly 22. The signal collection assembly 22 is connected to the lateral coupling bracket 21. The fiber optic assembly 30 connects the signal collection assembly 22 and the fluorescence detection module 10, so that the lateral scattering signal collected by the lateral coupling module 20 is transmitted to the fluorescence detection module 10, facilitating the fluorescence detection module 10 to collect and analyze the lateral scattering signal.

[0035] The signal collection component 22 includes: a coupling mirror module for coupling the lateral scattering signal. Specifically, the coupling mirror module can couple the lateral scattering signal generated by the light beam passing through the sample to be measured, so as to obtain the maximum lateral scattering signal, which is convenient for the subsequent fluorescence detection module 10 to collect and analyze these lateral scattering signals.

[0036] One end of the optical fiber component 30 is connected to the signal collection component 22 and the other end is connected to the fluorescence detection module 10, and the coupled lateral scattering signal is transmitted to the fluorescence detection module 10 for collecting and analyzing the lateral scattering signal. Specifically, one end of the signal collection component 22 away from the coupling mirror module is connected to one end of the optical fiber component 30. After the coupling mirror module couples the lateral scattering signal generated by the light beam passing through the sample to be measured, the coupled lateral scattering signal is transmitted to the optical fiber component 30; the other end of the optical fiber component 30 is connected to the fluorescence collection module, that is, the optical fiber component 30 connects the signal collection component 22 and the fluorescence detection module 10, so that the lateral scattering signal collected by the lateral coupling module 20 is transmitted to the fluorescence detection module 10, which is convenient for the fluorescence detection module 10 to collect and analyze the lateral scattering signal.

[0037] Thus, the signal collection component 22 and the fluorescence detection module 10 are connected by the optical fiber component 30, and the relative positions of the fluorescence detection module 10 and the signal collection component 22 can be adjusted, improving the flexibility of the installation of the fluorescence detection module 10.

[0038] According to Figure 1 and Figure 2 As shown, the lateral coupling module 20 further includes: an adjustment component, the adjustment component is arranged on the lateral coupling bracket 21, and the adjustment component is connected to the lateral coupling bracket 21 for adjusting the position of the signal collection component 22 so that the optical fiber component 30 can receive the lateral scattering signal coupled by the coupling mirror module.

[0039] The adjustment component can make the signal collection component 22 rotate around the axis of the lateral coupling bracket 21. Specifically, the axis of the lateral coupling bracket 21, that is, the axis of the signal collection component 22. Through the adjustment of the adjustment component, the signal collection component 22 can rotate around the axis to adjust the axial distance of the coupling mirror module, adjust the focal length of the coupling mirror module, and obtain the most accurate coupling state, so that the coupling mirror module couples the maximum lateral scattering signal.

[0040] In some embodiments, the adjustment component includes: a first adjustment component 23, the first adjustment component 23 can rotate relative to the lateral coupling bracket 21, and the first adjustment component 23 can make the signal collection component 22 rotate around the axis. The first adjustment component 23 is rotationally connected to the lateral coupling bracket 21, the first adjustment component 23 is fixedly connected to the signal collection component 22, and when the first adjustment component 23 rotates relative to the lateral coupling bracket 21, the signal collection component 22 can rotate accordingly.

[0041] In some other embodiments, the adjustment component can move the signal collection component 22 axially along the lateral coupling bracket 21. That is to say, along the axis of the lateral coupling bracket 21, which is also the axis of the signal collection component 22, under the action of the adjustment component, the signal collection component 22 can move axially along its own axis, so that the coupling mirror module moves axially along the lateral coupling bracket 21 to adjust the axial distance of the coupling mirror module, adjust the focal length of the coupling mirror module, obtain the most accurate coupling state, and enable the coupling mirror module to couple the maximum amount of laterally scattered signals.

[0042] In some other embodiments, the adjustment component includes: a second adjustment component. The second adjustment component can move the signal collection component 22 axially along the axis of the coupling mirror module. The second adjustment component can be an adjustment screw. By rotating the adjustment screw, the signal collection component 22 can move axially relative to the signal collection bracket, that is, the coupling mirror module moves axially to adjust the axial distance of the coupling mirror module, adjust the focal length of the coupling mirror module, obtain the most accurate coupling state, and enable the coupling mirror module to couple the maximum amount of laterally scattered signals.

[0043] Furthermore, the adjustment component can move the signal collection component 22 in the height direction. Specifically, under the action of the adjustment component, the signal collection component 22 can move in the height direction, so that the coupling mirror module moves in the height direction to adjust the height of the coupling mirror module, obtain the most accurate coupling state, and couple the maximum amount of laterally scattered signals.

[0044] In some other embodiments, the adjustment component includes: a third adjustment component 24. The third adjustment component 24 can move the signal collection component 22 in the height direction. The third adjustment component 24 can be an adjustment screw. By rotating the adjustment screw, the signal collection component 22 moves in the height direction to change the relative position of the coupling mirror module with respect to the sample to be measured, obtain the most accurate coupling state, and couple the maximum amount of laterally scattered signals.

[0045] Even further, the adjustment component can move the signal collection component 22 in the first direction. Specifically, under the action of the adjustment component, the signal collection component 22 can move in the first direction, so that the coupling mirror module moves in the first direction to adjust the relative position between the coupling mirror module and the sample to be measured, obtain the most accurate coupling state, and couple the maximum amount of laterally scattered signals.

[0046] In some other embodiments, the adjustment component includes: a fourth adjustment component 25. The fourth adjustment component 25 can move the signal collection component 22 in the first direction. The fourth adjustment component 25 can be an adjustment screw. By rotating the adjustment screw, the signal collection component 22 moves in the first direction to change the relative position of the coupling mirror module with respect to the sample to be measured, obtain the most accurate coupling state, and couple the maximum amount of laterally scattered signals.

[0047] By adjusting the components, the signal collection component 22 can be adjusted axially / circumferentially / vertically / in the first direction, with fast alignment speed and high coupling efficiency. This not only improves the analysis accuracy of the lateral detection mechanism 100, but also reduces the requirements for the machining and assembly accuracy of related parts.

[0048] As Figure 2 shown, the signal collection component 22 includes: an optical fiber connector 221, the optical fiber connector 221 is connected to the lateral coupling bracket 21, and the optical fiber connector 221 connects the coupling mirror module and the optical fiber component 30. Specifically, one end of the optical fiber connector 221 is connected to the coupling mirror module, the other end of the optical fiber connector 221 is connected to one end of the optical fiber component 30, and the other end of the optical fiber component 30 is connected to the fluorescence detection module 10. Then, the coupling mirror module can couple the lateral scattering signal and transmit it to the fluorescence detection module 10, and the fluorescence detection module 10 can collect and analyze the coupled lateral scattering signal.

[0049] Among them, the output end of the coupling mirror module is designed with a first interface flange, and one end of the optical fiber connector 221 is provided with a second interface flange 222. The first interface flange and the second interface flange 222 are connected, which is convenient for the connection between the coupling mirror module and the optical fiber connector 221.

[0050] As Figure 2 and Figure 3 shown, the optical fiber component 30 includes: a first connector 31 and multiple optical fibers 32. One end of the first connector 31 is connected to the optical fiber connector 221 and the other end is connected to one end of the multiple optical fibers 32, and the other ends of the multiple optical fibers 32 are connected to the fluorescence detection component. Specifically, the first connector 31 is connected between the optical fiber connector 221 and the multiple optical fibers 32, and the first connector 31 transmits the lateral scattering signal transmitted by the optical fiber connector 221 to the multiple optical fibers 32. One end of the first connector 31 is connected to the other end of the optical fiber connector 221, the other end of the first connector 31 is connected to one end of the multiple optical fibers 32, and the first connector 31 transmits the lateral scattering signal to the multiple optical fibers 32 respectively; the other ends of the multiple optical fibers 32 are connected to the fluorescence detection module 10,

[0051] For multiple lasers, there are multiple light spots for the coupled lateral scattering signal.

[0052] Therefore, the first connecting member 31 is provided with a plurality of first light-receiving holes 311 arranged vertically. The plurality of first light-receiving holes 311 are used to transmit the lateral scattering signals from the fiber optic connector 221 to the plurality of optical fibers 32. Specifically, the axial direction of the first connecting member 31 coincides with the axial direction of the coupling mirror module. Axially, the first light-receiving holes 311 penetrate through the first connecting member 31. The first light-receiving holes 311 receive the lateral scattering signals transmitted by the fiber optic connector 221. The lateral scattering signals form a plurality of light spots. One end of the first connecting member 31 is connected to the fiber optic connector 221, so one ends of the plurality of first light-receiving holes 311 respectively correspond to the plurality of light spots. The other end of the first connecting member 31 is connected to the plurality of optical fibers 32, so the plurality of optical fibers 32 respectively receive the plurality of light spots and transmit the corresponding lateral scattering signals to the fluorescence detection module 10.

[0053] Among them, the number of the optical fibers 32 is the same as the number of the first light-receiving holes 311. To ensure that the lateral scattering signals generated by different lasers are transmitted from different first light-receiving holes 311 through different optical fibers 32 to the fluorescence detection module 10, and finally the fluorescence detection module 10 collects and analyzes the lateral scattering signals.

[0054] As Figure 3 shown, the optical fiber assembly 30 further includes: a plurality of second connecting members 33, and the second connecting members 33 are respectively connected between the plurality of optical fibers 32 and the fluorescence detection module 10. Specifically, one ends of the plurality of optical fibers 32 are all connected to the first connecting member 31, the other ends of the plurality of optical fibers 32 are respectively connected to one ends of the plurality of second connecting members 33, and the other ends of the second connecting members 33 are connected to the fluorescence detection module 10. One second light-receiving hole is provided on any second connecting member 33. The second light-receiving hole receives the lateral scattering signal transmitted from the optical fiber 32 and transmits it to the fluorescence detection module 10.

[0055] Furthermore, the fluorescence detection module 10 includes: a mounting bracket 11 and a collimating mirror module 12. The collimating mirror module 12 is fixedly connected to the mounting bracket 11, and the other end of the optical fiber assembly 30 is connected to the collimating mirror module 12. Specifically, the collimating mirror module 12 is installed on the mounting bracket 11, the collimating mirror module 12 is fixedly connected to the mounting bracket 11, and the collimating mirror module 12 is connected to the other end of the optical fiber assembly 30, that is, the collimating mirror module 12 is connected to the other end of the second connecting member 33.

[0056] The collimating mirror module 12 includes: a collimating mirror bracket and a plurality of collimating mirrors. The plurality of collimating mirrors are installed on the collimating mirror bracket. The plurality of collimating mirrors are respectively connected to the other ends of the plurality of optical fibers 32, that is, the plurality of collimating mirrors are respectively connected to the other ends of the plurality of second connecting members 33. The collimating mirrors can be used to keep the lateral scattering signals transmitted by the optical fibers 32 parallel during subsequent transmission, so as to reduce energy loss and beam divergence.

[0057] The multiple collimating mirrors may be arranged at intervals in the height direction; or, the multiple collimating mirrors may be arranged in two columns in the height direction, wherein the multiple collimating mirrors in any column are arranged at intervals in the height direction, and the remaining multiple collimating mirrors in the other column are arranged at intervals in the height direction.

[0058] Combination Figures 3 - 5 As shown, the fluorescence detection module 10 also includes: a plurality of lens integrated brackets 13, the plurality of lens integrated brackets 13 are fixedly connected to the mounting bracket 11, and a plurality of first mounting grooves 131 inclined in the second direction are provided on the lens integrated bracket 13, and the first mounting grooves 131 are used to install the dichroic mirror 41 or the reflector 44. Specifically, the second direction is perpendicular to the third direction. The plurality of lens integrated brackets 13 are arranged at intervals along the third direction, and a plurality of first mounting grooves 131 are provided on any lens integrated bracket 13, and the plurality of first mounting grooves 131 are inclined relative to the second direction, and the first mounting grooves 131 are used to install the dichroic mirror 41 or the reflector 44. The plurality of lens integrated brackets 13 are arranged on the side of the collimator module 12 away from the second connecting member 33, and the plurality of mounting grooves correspond to the plurality of collimators respectively.

[0059] In some embodiments, a plurality of first mounting grooves 131 may be arranged sequentially along the height direction, thereby saving the space occupied by the lens integrated bracket 13 and improving space utilization.

[0060] Alternatively, the plurality of first mounting grooves 131 may be arranged in multiple rows and columns along the first direction and the height direction, so as to reduce the size of the lens integrated bracket 13 and improve space utilization.

[0061] The directions of at least two first mounting grooves 131 are different from the direction of at least another first mounting groove 131. The directions of the dichroic mirrors 41 or the reflective mirrors 44 in at least two first mounting grooves 131 on the same lens integrated bracket 13 are different from the direction of the dichroic mirrors 41 or the reflective mirrors 44 in at least another first mounting groove 131.

[0062] The lens integrated bracket 13 includes: a second mounting groove 132, and the second mounting groove 132 is used to mount the focusing lens 42. A second mounting groove 132 is correspondingly arranged for any first mounting groove 131. The second mounting grooves 132 and the first mounting grooves 131 are arranged at intervals. The number of the second mounting grooves 132 is the same as the number of the first mounting grooves 131. The axial direction of the focusing lens 42 is parallel to the second direction, and the direction of the second mounting groove 132 is parallel to the second direction, and the direction of the second mounting groove 132 is different from the direction of the first mounting groove 131.

[0063] The included angle between the second mounting groove 132 and the corresponding first mounting groove 131 may be 45°.

[0064] After the collimator module 12 transmits the lateral scattering signal to the dichroic mirror 41, the lateral scattering signal is reflected by the dichroic mirror 41 or the mirror 44 to the focusing mirror 42, and the focusing mirror 42 focuses the lateral scattering signal to facilitate the subsequent analysis of the lateral scattering signal.

[0065] As Figure 3 and Figure 4 shown, the fluorescence detection module 10 further includes: a plurality of detector modules 14, the plurality of detector modules 14 are fixedly connected to the mounting bracket 11 and respectively correspond to a plurality of collimators, the plurality of detector modules 14 are oppositely arranged on both sides of the base, and at least two detector modules 14 are arranged on the same side. Specifically, the number of detector modules 14 is the same as the number of lasers. Any one of the detector modules 14 includes: a plurality of detectors 141, and the heights of the plurality of detectors 141 in the same detector module 14 are the same. The number of detectors 141 is determined according to the wavelengths of the fluorescence generated after the different lasers excite the sample to be measured (the sample labeled with fluorescein).

[0066] In an embodiment of the present invention, the number of lasers is three, and three detector modules 14 are provided corresponding to the three lasers. The wavelengths of the fluorescence generated after the three lasers excite the sample to be measured (the sample labeled with fluorescein) are 2 bands, 4 bands, and 5 bands respectively. Therefore, one of the detector modules 14 is provided with 2 detectors 141, another detector module 14 is provided with 4 detectors 141, and another detector module 14 is provided with 5 detectors 141. Since the three collimators of the collimator module 12 are spaced in the height direction, the installation heights of the three detector modules 14 are not the same, and the three detector modules 14 form a layered structure, which is suitable for the fluorescence detection of the sample to be measured by the three lasers.

[0067] In practical applications, according to the types and numbers of lasers, the number of detector modules 14 and the number of detectors 141 in each detector module 14 are set. Therefore, the fluorescence detection module 10 of the embodiment of the present invention is suitable for the detection of multiple lasers and can meet more detection requirements.

[0068] The structure of the fluorescence detection module 10 is compact, effectively saving space, and can meet the diverse and personalized needs of users.

[0069] In an embodiment of the present invention, two of the three lasers correspond to detector modules 14 arranged on one side in the second direction, and the detector module 14 corresponding to the other laser is arranged on the other side in the second direction.

[0070] For the lateral scattering signal, due to its relatively weak intensity, a PMT detector 141 with strong signal amplification ability and high sensitivity can be used for analysis. The coupled lateral scattering signal is transmitted to the target surface of the PMT detector 141 to achieve signal acquisition and analysis.

[0071] The fluorescence detection module 10 further includes: a plurality of filter assemblies 15. The filter assembly 15 includes: a filter 43 bracket and a filter 43. The filter 43 is installed in the filter assembly 15, and the filter assembly 15 is arranged between the lens integration bracket 13 and the detector 141. The number of filter assemblies 15 is the same as that of the focusing lenses 42, and the plurality of filter assemblies 15 are respectively arranged opposite to the plurality of focusing lenses 42. Any one filter assembly 15 corresponds to one of the detectors 141. The lateral scattering signal transmitted from the collimator is focused by the focusing lens 42 and filtered by the filter 43 and then transmitted to the detector 141, and the detector 141 analyzes the lateral scattering signal.

[0072] In the lateral detection mechanism 100 of the present utility model, an optical fiber 32 is used for coupling the lateral scattering signal, and the collection efficiency of the lateral scattering signal is high. The manufacturing and installation precision requirements for related parts are not high, and the coupling is simple and easy to implement. In addition, for a system with multiple lasers, the multiple detector modules 14 of the fluorescence detection module 10 adopt a layered structure, with a compact layout and high integration.

[0073] According to an optical detection device of the second aspect embodiment of the present utility model, it includes: a flow cell, which is used for the sample to be tested in the detection sample liquid to queue up and pass under the entrainment of the diluent. The flow cell provides a place where the sample to be tested is covered by the light source, so that after the light source irradiates the sample to be tested in the flow cell, it is convenient to analyze the information of the sample to be tested.

[0074] The optical detection device further includes: a light source, which is used for emitting light. The optical fiber emitted by the light source is directed towards the sample to be tested flowing through the flow cell 21, facilitating subsequent analysis of the information of the sample to be tested.

[0075] The optical detection device further includes: a forward detection mechanism, which is used for collecting the forward scattering signal generated when the light source irradiates the sample to be tested.

[0076] The optical detection device further includes: a lateral detection mechanism 100, which is used for collecting the lateral scattering signal generated when the light source irradiates the sample to be tested.

[0077] The optical detection device further includes: a sampling device, which is connected between the sample tube and the flow cell bracket. The sampling device sucks the sample to be tested in the sample tube and transports the sample to be tested to the flow cell, facilitating the acquisition and analysis of the information of the sample to be tested.

[0078] As a preferred implementation of this embodiment, the working principle of the optical detection device is as follows: The light beam emitted by the laser forms an elliptical light spot at the central position of the flow cell. The short axis direction of the elliptical light spot is consistent with the flow direction of the sample to be measured (the sample labeled with fluorescein), and the long axis direction is perpendicular to the flow direction of the sample to be measured. When the sample to be measured passes through the light beam irradiation area (the elliptical light spot at the center of the flow cell), the forward scattering signal and the lateral scattering signal generated by the scattering of the sample to be measured are collected by the forward detection mechanism and the lateral detection mechanism 100, and finally the information of the sample to be measured is obtained.

[0079] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention.

[0080] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "schematic embodiments", "examples", "specific examples", or "some examples", etc. means that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example.

[0081] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principle and spirit of the present invention, and the scope of the present invention is defined by the claims and their equivalents.

Claims

1. A lateral detection mechanism, characterized in that: include: Fluorescence detection module (10); A lateral coupling module (20), wherein the lateral coupling module (20) and the fluorescence detection module (10) are arranged at an interval from each other, and the lateral coupling module (20) comprises: A lateral coupling bracket (21); A signal collection component (22) comprising: a coupling mirror module for coupling side scattering signals, the signal collection component (22) being connected to the side coupling bracket (21); An optical fiber component (30), one end of the optical fiber component (30) is connected to the signal collection component (22) and the other end is connected to the fluorescence detection module (10), transmitting the coupled side scattering signal to the fluorescence detection module (10) to collect and analyze the side scattering signal.

2. The lateral detection mechanism according to claim 1, characterized in that: The lateral coupling module (20) further comprises: an adjustment component, wherein the adjustment component is arranged on the lateral coupling bracket (21), The adjustment component can enable the signal collection component (22) to rotate around the axial direction of the lateral coupling bracket (21); and / or, The adjustment component can enable the signal collection component (22) to move along the axial direction of the lateral coupling bracket (21), and / or, The adjustment component can move the signal collection component (22) in a height direction; and / or, The adjustment component can enable the signal collection component (22) to move along a first direction.

3. The lateral detection mechanism according to claim 1, characterized in that: The signal collection component (22) comprises: an optical fiber connector (221), the optical fiber connector (221) being connected to the lateral coupling bracket (21), and the optical fiber connector (221) connecting the coupling mirror module and the optical fiber component (30).

4. The lateral detection mechanism according to claim 3, characterized in that: The optical fiber assembly (30) comprises: a first connector (31) and a plurality of optical fibers (32); one end of the first connector (31) is connected to the optical fiber connector (221) and the other end is connected to one end of the plurality of optical fibers (32); the other ends of the plurality of optical fibers (32) are connected to the fluorescence detection module (10).

5. The lateral detection mechanism according to claim 4, characterized in that: The first connecting member (31) is provided with a plurality of vertically arranged first light receiving holes (311), and the plurality of first light receiving holes (311) are used to transmit side scattered signals from the optical fiber connecting member (221) to the plurality of optical fibers (32).

6. The lateral detection mechanism according to claim 1, characterized in that: The fluorescence detection module (10) comprises: a mounting bracket (11) and a collimator lens module (12); the collimator lens module (12) is fixedly connected to the mounting bracket (11); the other end of the optical fiber assembly (30) is connected to the collimator lens module (12); the collimator lens module (12) comprises: a collimator lens bracket and a plurality of collimators; the plurality of collimators are mounted on the collimator lens bracket.

7. The lateral detection mechanism according to claim 6, characterized in that: The fluorescence detection module (10) further comprises: a plurality of lens integrated brackets (13), wherein the plurality of lens integrated brackets (13) are fixedly connected to the mounting bracket (11); The lens integrated bracket (13) is provided with a plurality of first mounting grooves (131) obliquely arranged in a second direction, the first mounting grooves (131) being used to mount a dichroic mirror (41) or a reflective mirror (44), and the direction of at least two of the first mounting grooves (131) is different from the direction of at least another first mounting groove (131); The lens integrated bracket (13) is also provided with a second mounting groove (132) spaced apart from the first mounting groove (131) and used for mounting a focusing lens (42).

8. The lateral detection mechanism according to claim 7, characterized in that: The fluorescence detection module (10) further comprises: a plurality of detector modules (14), the plurality of detector modules (14) being fixedly connected to the mounting bracket (11) and respectively corresponding to the plurality of collimating lenses, the plurality of detector modules (14) being arranged opposite to each other on two sides of the mounting bracket (11), wherein at least two of the detector modules (14) are arranged on the same side.

9. An optical detection device, characterized in that: include: A flow chamber, the flow chamber is used for allowing the sample to be tested to pass through and providing a place for the sample to be tested to be irradiated by a light source; A light source, the light source is used to emit light and incident on the sample to be tested flowing through the flow chamber; The lateral detection mechanism according to any one of claims 1 to 8 is used to collect the side scattering signal generated when the light source irradiates the sample to be tested.

10. An optical detection device according to claim 9, characterized in that: Also includes: Sample loading device, The sample loading device is connected to the flow chamber and is used for drawing the sample to be tested from the sample tube and transporting it to the flow chamber.

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