Enzyme substrate coliform group analysis system
By designing an enzyme substrate coliform analysis system that integrates label management, mixing device and incubator room functions, the existing system functions are scattered and complicated, and efficient sample analysis and traceability management are achieved, which improves experimental results and system practicality.
Patent Information
- Application Number
- CN202421542084.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-01
- Publication Date
- 2025-06-17
- Estimated Expiration
- 2034-07-01
AI Technical Summary
The existing enzyme substrate coliform analysis system has scattered functions, complicated processes, many steps and is not completed in the same location, resulting in difficulty in traceability and poor experimental results.
An enzyme substrate coliform analysis system including label editing equipment, label printer, label scanner, mixing device, quantitative disk, quantitative disk sealing device, incubator room, camera and lamp ring group was designed. Sample traceability is realized through label management, and the mixing device and incubator room are optimized for sample processing and culture process.
This system improves the efficiency of coliform analysis of enzyme substrates, realizes accurate sample traceability and information management, improves experimental results, and has better system practicality.
Smart Images
Figure CN222990121U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of experimental equipment, in particular to an enzyme substrate coliform analysis system. Background Art
[0002] The enzyme substrate method is a method for studying the metabolic ability of microorganisms, and the degradation ability of microorganisms to specific substrates is measured by adding different types of substrates. In the analysis of coliform bacteria, the enzyme substrate method can be used to evaluate the degradation activity of coliform bacteria to different substrates, so as to understand its metabolic function and community structure.
[0003] In the related art, the existing enzyme substrate coliform analysis systems are often scattered functional modules. During the process of sample sampling, detection and analysis, due to the cumbersome process, many steps and not being completed in the same place, it is difficult to trace the source, the operation process is cumbersome, and it is easy to cause the experimental effect to fail to meet the expectations.
[0004] In summary, the problems existing in the related art need to be solved urgently. Summary of the Utility Model
[0005] The purpose of the utility model is to solve at least to some extent one of the technical problems existing in the related art.
[0006] To this end, an object of the utility model is to provide an enzyme substrate coliform analysis system.
[0007] In order to achieve the above technical purpose, the technical solutions adopted by the utility model include:
[0008] On the one hand, an embodiment of the utility model provides an enzyme substrate coliform analysis system, including:
[0009] A label editing device, a label printer, a label scanner, a mixing device, a dosing tray, a dosing tray sealing device, an incubator chamber, a camera and a lamp ring group;
[0010] The label editing device is used to edit the label corresponding to the sample bottle, the label printer is used to print the label, and the label scanner is used to scan the label;
[0011] The mixing device is used to mix the sample and the culture reagent added to the sample bottle, and pour the mixed sample and culture reagent into the dosing tray; the dosing tray sealing device is used to seal the dosing tray;
[0012] The incubator chamber is used to place the dosing tray, the lamp ring group is arranged in the reading area of the enzyme substrate coliform analysis system, and the camera is used to take pictures of the dosing tray in the reading area.
[0013] In addition, an enzyme substrate coliform analysis system according to the above embodiments of the present utility model may further have the following additional technical features:
[0014] Further, in an embodiment of the present utility model, the enzyme substrate coliform analysis system further includes a terminal device, and the label editing device, the label printer, and the label scanner are connected to the terminal device.
[0015] Further, in an embodiment of the present utility model, the terminal device is a computer.
[0016] Further, in an embodiment of the present utility model, the mixing device includes a manipulator and a capping device.
[0017] Further, in an embodiment of the present utility model, the quantitative plate sealing device includes an inlet tray, a sealing roller, and an outlet tray.
[0018] Further, in an embodiment of the present utility model, the enzyme substrate coliform analysis system includes a heating device and a temperature sensor;
[0019] The heating device and the temperature sensor are arranged at the incubator chamber.
[0020] Further, in an embodiment of the present utility model, the incubator chamber includes a plurality of detachable brackets, and the detachable brackets are used for placing the quantitative plate.
[0021] Further, in an embodiment of the present utility model, the lamp ring group includes an ultraviolet fluorescent lamp ring and a white light LED light bar.
[0022] The advantages and beneficial effects of the present utility model will be partially given in the following description, partially become obvious from the following description, or be understood through the practice of the present utility model:
[0023] The present application discloses an enzyme substrate coliform analysis system, which includes a label editing device, a label printer, a label scanner, a mixing device, a quantitative plate, a quantitative plate sealing device, an incubator chamber, a camera and a lamp ring group; the label editing device is used for editing the label corresponding to the sample bottle, the label printer is used for printing the label, and the label scanner is used for scanning the label; the mixing device is used for mixing the sample and the culture reagent added in the sample bottle, and pouring the mixed sample and culture reagent into the quantitative plate; the quantitative plate sealing device is used for sealing the quantitative plate; the incubator chamber is used for placing the quantitative plate, the lamp ring group is arranged in the reading area of the enzyme substrate coliform analysis system, and the camera is used for taking pictures of the quantitative plate in the reading area. This system can efficiently realize the analysis and application of enzyme substrate coliform, and can achieve accurate sample traceability and information management based on the label. The practicability of the system is better, which is beneficial to improving the experimental effect. Description of the Drawings
[0024] Figure 1 Fig. shows a schematic structural diagram of an enzyme substrate coliform analysis system provided in an embodiment of the present application. Detailed Embodiments
[0025] The embodiments of the present invention will be described in detail below. The examples of the embodiments are shown in the drawings, in which the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions from beginning to end. The embodiments described below with reference to the drawings are exemplary and are only used to explain the present invention, and should not be construed as a limitation of the present invention.
[0026] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by the terms "length", "upper", "lower", "front", "rear", "left", "right", "top", "inner", "outer", "axial", "radial", "circumferential", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention. In addition, the features defined as "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present invention, unless otherwise specified, the meaning of "a plurality" is two or more.
[0027] In the description of the present utility model, it should be noted that unless otherwise clearly specified and defined, the terms "set", "installed", "connected", and "coupled" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations.
[0028] The enzyme substrate method is a method for studying the metabolic ability of microorganisms, and the degradation ability of microorganisms to specific substrates is measured by adding different types of substrates. In the analysis of coliform bacteria, the enzyme substrate method can be used to evaluate the degradation activity of coliform bacteria to different substrates, so as to understand its metabolic function and community structure.
[0029] In the related art, the existing enzyme substrate coliform analysis systems are often scattered functional modules. In the process of sample sampling, detection, and analysis, due to the cumbersome process, many steps and not being completed in the same place, it is difficult to trace the source, the operation process is cumbersome, and it is easy to cause the experimental effect to fail to meet the expectations.
[0030] In view of this, an enzyme substrate coliform analysis system is provided in an embodiment of the present application, including a label editing device, a label printer, a label scanner, a mixing device, a dosing tray, a dosing tray sealing device, an incubator chamber, a camera, and a lamp ring group; the label editing device is used to edit the label corresponding to the sample bottle, the label printer is used to print the label, and the label scanner is used to scan the label; the mixing device is used to mix the sample and the culture reagent added to the sample bottle, and pour the mixed sample and culture reagent into the dosing tray; the dosing tray sealing device is used to seal the dosing tray; the incubator chamber is used to place the dosing tray, the lamp ring group is arranged in the reading area of the enzyme substrate coliform analysis system, and the camera is used to take pictures of the dosing tray in the reading area. This system can efficiently realize the analysis and application of enzyme substrate coliform bacteria, and can accurately trace the samples and manage the information based on the labels, and the practicability of the system is better, which is beneficial to improving the experimental effect.
[0031] Next, with reference to specific drawings, an enzyme substrate coliform analysis system provided in an embodiment of the present application will be described in detail.
[0032] An enzyme substrate coliform analysis system is provided in an embodiment of the present application, which can be applied to the technical field of experimental equipment. Specifically, an enzyme substrate coliform analysis system provided in an embodiment of the present application mainly includes:
[0033] Label editing device, label printer, label scanner, mixing device, dosing tray, dosing tray sealing device, incubator chamber, camera and lamp ring group;
[0034] The label editing device is used to edit the label corresponding to the sample bottle, the label printer is used to print the label, and the label scanner is used to scan the label;
[0035] The mixing device is used to mix the sample and the culture reagent added to the sample bottle, and pour the mixed sample and culture reagent into the dosing tray; the dosing tray sealing device is used to seal the dosing tray;
[0036] The incubator chamber is used to place the dosing tray, the lamp ring group is arranged in the reading area of the enzyme substrate coliform analysis system, and the camera is used to take pictures of the dosing tray in the reading area.
[0037] In the embodiments of the present application, an enzyme substrate coliform analysis system is provided, which includes a label editing device, a label printer, a label scanner, a mixing device, a dosing tray, a dosing tray sealing device, an incubator chamber, a camera and a lamp ring group. Specifically, the label editing device therein can be used by experimental personnel to edit the label corresponding to the sample bottle. For example, it can include information such as the name of the sample contained in the sample bottle, collection time, location, number, collector, etc. The present application does not limit this. The label editing device can be a touch screen device or other devices that can be used for human-computer interaction to receive the information input by the user to edit the label content. The label printer can be used to print the label corresponding to the sample bottle edited by the experimental personnel. After the label is printed, the experimental personnel can stick it on the corresponding sample bottle for subsequent separation and information collection. In the embodiments of the present application, the label scanner can be used to scan the label stuck on the sample bottle, so as to obtain the information corresponding to the sample bottle. The label scanner can use a barcode scanner, but is not limited to this type. Exemplarily, in some embodiments, the enzyme substrate coliform analysis system may further include a terminal device, such as a computer. The label editing device, the label printer and the label scanner can be connected to the terminal device. In this way, it is convenient to store the relevant information in the terminal device for data traceability or verification.
[0038] In the embodiments of the present application, the enzyme substrate coliform analysis system further includes devices related to experiments. After the experimenter processes the corresponding label of the sample bottle, the corresponding sample and culture reagent can be added to the sample bottle, and then the mixing device provided in the enzyme substrate coliform analysis system is used for uniform mixing. Then, the mixing device can pour the mixed sample and culture reagent into the quantitative tray. In the embodiments of the present application, the enzyme substrate coliform analysis system further includes a quantitative tray sealing device, which can be used to seal the quantitative tray. The sealed quantitative tray can be placed in the incubator chamber for cultivation. After cultivation, the experimenter can identify it in the reading area of the enzyme substrate coliform analysis system. In the reading area, a lamp ring group and a camera are provided. Among them, the lamp ring group can be used for lighting to facilitate analysis, and the camera can collect the image of the quantitative tray in the reading area, so as to record the results related to the experiment.
[0039] The following describes and explains the enzyme substrate coliform analysis system provided in the embodiments of the present application with reference to a specific embodiment.
[0040] In the embodiments of the present application, an enzyme substrate coliform analysis system is provided, which may include a label editing device, a label printer, a label scanner, and a corresponding terminal device. The terminal device can serve as the host of the system. The experimenter can edit the relevant label through the label editing device, and then send it to the label printer for printing. The printed label can be pasted onto the corresponding sample bottle. The label scanner can be used to read the relevant information on the label, store the read information in the host, and realize the recording of information for convenient subsequent traceability.
[0041] Refer to Figure 1 , Figure 1 shows a schematic diagram of the components of an enzyme substrate coliform analysis system provided in the embodiments of the present application. In the embodiments of the present application, the manipulator 10 and the capping device can form a mixing device. The manipulator 10 can be fixed on the bracket 11. Specifically, when the experimenter conducts an experiment, the sample bottle can be placed in the reagent adding area 2. The manipulator 10 can move the sample bottle containing the sample to the capping device, unscrew the bottle cap, add the configured culture reagent to the sample, and then screw the bottle cap back on. The manipulator 10 automatically shakes the sample bottle to mix it evenly. In the embodiments of the present application, the manipulator 10 can also take a new quantitative tray from the quantitative tray storage tray 1 and stand it at the fixing device 3, open the cap of the mixed sample bottle, pour it into the quantitative tray, place the quantitative tray on the quantitative tray silica gel tray, and send it to the sealing device.
[0042] In the embodiments of the present application, the quantitative plate sealing device includes an inlet tray 4, a sealing roller 5, and an outlet tray 6. The quantitative plate can be placed at the inlet tray 4. Under the action of the motor of the sealing device, the quantitative plate undergoes a sealing operation through the sealing roller 5. Particularly, in some embodiments, the sealing device may include a button assembly for setting the temperature of the sealing roller 5 and the rotational speed of the motor. Selecting different parameters can adapt to different materials, which can not only ensure that the bacteria will not be inactivated but also ensure the sealing effect of the quantitative plate. The main manifestations are as follows: 1. For different quantitative plates, the amount of the mixed reagent poured is different. To ensure the sealing effect, there will be some differences in temperature during sealing; 2. Different quantitative plates or different mixed reagents have different heat resistance. Under the condition of appropriate temperature, adjusting the motor to complete the sealing of the quantitative plate as soon as possible can reduce the possibility of the temperature damaging the microorganisms in the mixed reagent. The quantitative plate passing through the sealing roller 5 exits from the outlet tray 6 of the sealing device. The manipulator 10 can be used to take out the quantitative plate and place it on the quantitative plate culture rack; then the manipulator 10 moves the silicone tray of the quantitative plate back to the front of the sealing device and waits to be used when the next sample bottle enters the sealing device.
[0043] In the embodiments of the present application, the sealed quantitative plate can be placed in the incubator chamber 12. Specifically, the incubator chamber 12 may include a plurality of detachable brackets, and each detachable bracket is used to place the quantitative plate. Due to the detachable setting, it is convenient to clean areas such as the incubator chamber 12 and the bottom, which enables the entire incubator chamber 12 to ensure cleanliness and reduce the probability of sample contamination. In the embodiments of the present application, the incubator chamber 12 can be set to maintain constant temperature and humidity. For example, in some embodiments, a temperature control pipe 13 can be provided around the incubator chamber 12. The temperature control pipe 13 may include a heating device and a temperature sensor. The temperature sensor can detect the temperature at the incubator chamber 12, and the heating device can perform a steady-state output of the temperature, so that the incubator chamber 12 reaches a constant temperature state. A similar method can be used for the treatment of constant humidity, which will not be elaborated here.
[0044] In the embodiments of the present application, an experimenter can set the culture time and detection requirements of the quantitative plate through system operations. After reaching the set time length, the cultured quantitative plate can be moved to the reading area 7. A high-resolution camera 9 is installed at the center directly above the reading area 7, and its field of view can cover the entire reading area 7. The quantitative plate in the reading area 7 can be photographed, and then further image analysis and other operations can be carried out. For example, image recognition applications in the field of artificial intelligence can be combined to perform image segmentation and recognition, so as to obtain corresponding analysis results. In the embodiments of the present application, relevant lamp ring groups 8 can be installed above the reading area 7. The lamp ring groups 8 can facilitate the identification of bacterial colonies. Specifically, the lamp ring groups 8 in the embodiments of the present application can include ultraviolet fluorescent lamp rings and white light LED light strips. Among them, there can be multiple ultraviolet fluorescent lamp rings. For example, it can include an ultraviolet fluorescent lamp ring with a wavelength of 254 nm and an ultraviolet fluorescent lamp ring with a wavelength of 365 nm. Moreover, corresponding wavelength filters can be installed below the ultraviolet fluorescent lamp rings to reduce the influence of other wavelengths.
[0045] In the embodiments of the present application, the quantitative plate cultured in the incubator chamber 12 for a certain period of time can be placed in the reading area 7. Under the irradiation of the white light LED light strip, a picture of the quantitative plate under white light can be taken, and the number and corresponding positions of the yellow holes on the current quantitative plate can be counted and stored; then the white light LED light strip is turned off, and the 365-nm ultraviolet fluorescent lamp ring is turned on. Under the irradiation of the ultraviolet fluorescent lamp ring, another picture of the quantitative plate at this time is taken, and the number and positions of the holes emitting fluorescence are counted and saved. These data can be used for relevant analysis. By comparing with the set MPN table of the quantitative plate, the final situation can be obtained. It should be noted that in the embodiments of the present application, the action of sending the quantitative plate from the incubator chamber 12 to the reading area 7 for analysis is not limited to a single time. It can also be taken out for analysis during the culture process; and when taking it out for analysis during the culture process, it is not necessary to use ultraviolet light irradiation. Whether to turn on the ultraviolet fluorescent lamp ring and whether to turn on the white light LED light strip can be set arbitrarily according to the actual situation.
[0046] Exemplarily, taking the analysis application of fecal coliform as an example, which requires culturing at 44.5 °C for 24 hours. It can be set in the system to use a robotic arm to take out the quantitative plate in the incubator chamber 12 and place it in the reading area 7 every 1 hour. By turning on the white light LED strip, take a photo of the quantitative plate at this time, count and store the number and position of the current yellow holes, and then put the quantitative plate back into the culture chamber for continued culturing. Repeat this process. After the culturing time reaches 24 hours, take it out again and perform the above operations, and then irradiate it with a 365 nm ultraviolet fluorescent lamp, count and store the number and position of the holes emitting fluorescence at this time. In this way, the change in the number and position of the yellow holes under the white light LED throughout the process and the number of holes emitting fluorescence under the final ultraviolet fluorescent lamp can be saved, which can effectively improve the accuracy of the final reading; and the change situation of the quantitative plate throughout the process can be understood, which is helpful for analyzing the growth situation of fecal coliform. The relevant information can be stored in the system for convenient data traceability of the entire experiment, or these data can be printed out for paper-based archiving. This application does not limit this.
[0047] In the description of this specification, the description with reference to terms such as "one embodiment", "another embodiment" or "certain embodiments" means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in a suitable manner in any one or more embodiments or examples.
[0048] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and purposes of the present invention. The scope of the present invention is defined by the claims and their equivalents.
Claims
1. An enzyme substrate coliform group analysis system, characterized in that: include: Label editing equipment, label printer, label scanner, mixing device, quantitative tray, quantitative tray sealing device, incubator chamber, camera and light ring set; The label editing device is used to edit the label corresponding to the sample bottle, the label printer is used to print the label, and the label scanner is used to scan the label; The mixing device is used to mix the sample and the culture reagent added in the sample bottle, and pour the mixed sample and the culture reagent into the quantitative plate; the quantitative plate sealing device is used to seal the quantitative plate; The incubator chamber is used for placing the quantitative disk, the light ring group is arranged in the reading area of the enzyme substrate coliform group analysis system, and the camera is used for taking pictures of the quantitative disk in the reading area.
2. An enzyme substrate coliform group analysis system according to claim 1, characterized in that: The enzyme substrate coliform group analysis system also includes a terminal device, and the label editing device, the label printer and the label scanner are connected to the terminal device.
3. An enzyme substrate coliform group analysis system according to claim 2, characterized in that: The terminal device is a computer.
4. The enzyme substrate coliform group analysis system according to claim 1, characterized in that: The mixing device comprises a mechanical arm and a capping device.
5. The enzyme substrate coliform group analysis system according to claim 1, characterized in that: The quantitative tray sealing device comprises an inlet tray, a sealing roller and an outlet tray.
6. The enzyme substrate coliform group analysis system according to claim 1, characterized in that: The enzyme substrate coliform group analysis system includes a heating device and a temperature sensor; The heating device and the temperature sensor are arranged at the incubator chamber.
7. The enzyme substrate coliform group analysis system according to claim 6, characterized in that: The incubator chamber includes a plurality of detachable brackets, and the detachable brackets are used to place the quantitative tray.
8. The enzyme substrate coliform group analysis system according to claim 1, characterized in that: The lamp ring group includes an ultraviolet fluorescent lamp ring and a white light LED lamp strip.