Cross type lifting power module and antigen repair device

Abstract

The utility model discloses a cross type lifting power module and an antigen repairing device, and belongs to the technical field of biomedical experiment equipment. The crossed lifting power module is suitable for being applied to the antigen repair device. The crossed lifting power module comprises a driving mechanism, a crossed frame and a lifting mechanism; the crossed frame comprises two supports which are arranged in a crossed mode and hinged to each other, the driving mechanism is in transmission connection with the first frame ends of the supports, the two second frame ends of the crossed frame are both in transmission connection with the lifting mechanism, the lifting mechanism comprises a gland, and the gland is used for sealing an opening of the inner container. The antigen repair device comprises the crossed lifting power module, an inner container, a heating assembly and the like. The driving mechanism drives the cross frame to open or close so as to drive the gland to seal or open the first opening of the inner container, so that the time required for repairing the antigen in the inner container can be shortened, the steps needing manual operation in the antigen repairing process are reduced, and the antigen repairing efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of biomedical experimental equipment, in particular to a cross-type lifting power module and an antigen repair device. Background Art

[0002] In immunohistochemistry experiments, antigen retrieval is a necessary step. During antigen retrieval, chemical reagents need to be added to the tissue sample on the slide and heated. The blocked antigens or antigens with distorted peptide chains can be exposed again by the action of the reagents and heat.

[0003] However, the antigen repair method in the related art heats the tissue sample on the slide by means of hot plate heating, water bath heating, etc., but the entire antigen repair process is time-consuming and inefficient. Some laboratories also have an additional pressure cooker, which is used to perform high-temperature water bath antigen repair inside the pressure cooker by placing the slide containing the tissue section into the pressure cooker. However, the pressure cooker must be manually operated by the operator. However, when the workload of the immunohistochemistry experiment is large, manual operation is inefficient. Utility Model Content

[0004] The purpose of the embodiments of the utility model is to provide a cross-type lifting power module and an antigen repair device, in which the pressure cover can seal or open the opening of the inner container under the drive of the cross-type lifting power module, without the need for manual sealing or opening, which can reduce manual operations, shorten the time required for antigen repair, and improve the efficiency of antigen repair.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] A cross-type lifting power module is applied to an antigen repair device; the cross-type lifting power module comprises:

[0007] Driving mechanism;

[0008] A lifting mechanism, comprising a pressing cover, the pressing cover is used to seal the opening of the container;

[0009] A cross frame, comprising two brackets; the two ends of the bracket are a first bracket end and a second bracket end, the bracket comprises a hinged portion located between the first bracket end and the second bracket end, and the hinged portions of the two brackets are hinged; the second bracket ends of the two brackets in the cross frame are both transmission-connected to the lifting mechanism;

[0010] The output end of the driving mechanism is drivingly connected to the first frame end of the bracket, and the driving mechanism is used to drive the two brackets in the cross frame to open or close, so as to drive the lifting mechanism to rise or fall.

[0011] Optionally, two of the cross frames are included, and the two cross frames are arranged at intervals.

[0012] Optionally, the lifting mechanism includes a transmission seat, the gland is connected to the transmission seat, and the second frame ends of each of the brackets are all in transmission connection with the transmission seat.

[0013] Optionally, the transmission seat is provided with a chute, and the cross-shaped lifting power module includes a sliding rod;

[0014] The first first frame end of the first cross frame is connected to the first first frame end of the second cross frame through at least one sliding rod; the second first frame end of the second cross frame is connected to the second first frame end of the second cross frame through at least one sliding rod; the sliding rod is slidably installed in the chute.

[0015] Optionally, a lead screw assembly is further included; the lead screw assembly includes a lead screw, a first bushing, and a second bushing. The lead screw is provided with a first rod section and a second rod section with opposite spiral directions. The first bushing is threadedly connected to the first rod section, and the second bushing is threadedly connected to the second rod section; when the lead screw rotates, the first bushing and the second bushing approach or move away from each other;

[0016] The cross-shaped lifting power module further includes a housing; the first end of the lead screw is in transmission connection with the driving mechanism, and the second end of the lead screw is rotatably connected to the housing; the two first frame ends in the cross frame are respectively connected to the first bushing and the second bushing.

[0017] Optionally, a reduction gearbox is further included. The output end of the driving mechanism is in transmission connection with the input end of the reduction gearbox, and the output end of the reduction gearbox is in transmission connection with the first end of the lead screw; the reduction gearbox includes a worm and a worm gear. The driving mechanism is in transmission connection with the worm, the worm gear is in transmission connection with the worm, and the worm gear is in transmission connection with the first end of the lead screw;

[0018] And / or, the driving mechanism is a stepper motor.

[0019] Optionally, a housing and a transverse guide rail installed on the housing are further included. One or both of the first frame ends of the two brackets of the cross frame are slidably connected to the transverse guide rail; when the cross frame opens or closes, the first frame end slides along the transverse guide rail.

[0020] Optionally, a housing and a vertical guide rail installed on the housing are further included. At least one hinge part of the cross frame is slidably connected to the vertical guide rail through a connecting shaft; when the cross frame opens or closes, the connecting shaft slides along the vertical guide rail.

[0021] Optionally, it further includes a connecting shaft, and the hinge parts of the two brackets in the cross-shaped frame are rotatably installed on the connecting shaft, and both ends of the connecting shaft are slidably connected to the two vertical guide rails.

[0022] An antigen repair device includes a cross-shaped lifting power module as described in the above solution, and further includes an inner container and a gland;

[0023] An inner water tank is provided inside the inner container, and a first opening communicating with the inner water tank is provided on one side of the inner container; the transmission seat is connected to the gland, and the lifting power module is used to drive the gland to move from a first position to a second position. When the gland is in the first position, the gland is separated from the inner container; when the gland is in the second position, the gland seals the first opening.

[0024] The beneficial effects of the present utility model are as follows:

[0025] This cross-shaped lifting power module can be applied to an antigen repair device with an inner container. By driving the cross-shaped frame to open or close through a driving mechanism, the gland can be driven to move, so that the gland seals or opens the first opening of the inner container, realizing the sealing or opening of the internal space of the inner container. When the gland seals the inner container and the heating component heats the inner container, the air pressure and temperature inside the inner container increase, which can shorten the time required for antigen repair inside the inner container, and reduce the steps that need to be manually operated in the antigen repair process, thereby improving the antigen repair efficiency as a whole.

[0026] This cross-shaped lifting power module can apply uniform pressure to the gland through the cross-shaped frame, and the movement track of the gland is stable.

[0027] This antigen repair device can repair antigens by means of high-pressure heating, shorten the time required for antigen repair, improve the experimental efficiency, and enhance the repair effect; moreover, multiple slides can be accommodated in the inner container at the same time, and tissues on multiple slides can be subjected to antigen repair simultaneously, improving the experimental efficiency. Description of the Drawings

[0028] The following further describes the present utility model in detail according to the drawings and embodiments.

[0029] Figure 1 It is a schematic structural diagram of the heating device according to an embodiment of the present utility model;

[0030] Figure 2 It is an exploded view of the heating device according to an embodiment of the present utility model (some structures are omitted in the figure);

[0031] Figure 3Cross-sectional view of the heating device according to the embodiment of the present utility model;

[0032] Figure 4 Schematic diagram of the internal structure of the heating device according to the embodiment of the present utility model (partial housing is omitted in the figure);

[0033] Figure 5 One of the schematic diagrams of the lifting power module when the gland is in the first position in the heating device according to the embodiment of the present utility model;

[0034] Figure 6 Another schematic diagram of the lifting power module when the gland is in the first position in the heating device according to the embodiment of the present utility model;

[0035] Figure 7 The third schematic diagram of the lifting power module when the gland is in the first position in the heating device according to the embodiment of the present utility model;

[0036] Figure 8 Partial structural decomposition schematic diagram of the lifting power module in the heating device according to the embodiment of the present utility model;

[0037] Figure 9 One of the schematic diagrams of the positional relationship among the gland, the wafer carrier, the inner container, and the outer container when the gland is in the first position in the heating device according to the embodiment of the present utility model;

[0038] Figure 10 For Figure 9 Enlarged view of part A in

[0039] Figure 11 Schematic diagram of the cooperation relationship among the gland, the wafer carrier, the inner container, the outer container, and the heating components when the gland is in the second position in the heating device according to the embodiment of the present utility model;

[0040] Figure 12 For Figure 11 Enlarged view of part B in

[0041] Figure 13 For Figure 11 Enlarged view of part C in

[0042] Figure 14 Schematic diagram of the cooperation relationship among the inner container, the outer container, and the heating components in the heating device according to the embodiment of the present utility model.

[0043] In the figure: 10, inner container; 101, inner water tank; 102, first opening; 103, first diversion slope; 104, second diversion slope; 11, inner chamber body; 12, inner bottom plate; 121, substrate; 122, second boss; 13, liquid channel; 14, first mounting seat; 15, second mounting seat;

[0044] 20. Outer container; 201. Outer water tank; 202. Second opening; 21. Outer housing; 22. Outer bottom plate;

[0045] 30. Pressing cover; 31. Cover body; 32. First boss;

[0046] 41. Driving mechanism; 42. Cross frame; 421. Bracket; 422. First end of the frame; 423. Second end of the frame; 424. Hinge part; 43. Transmission seat; 431. Slide groove; 441. Connecting shaft; 442. Sliding rod; 451. Lead screw; 452. First bushing; 453. Second bushing; 461. Horizontal guide rail; 462. Vertical guide rail; 47. Reduction box;

[0047] 50. Heating component; 61. First seal; 62. Second seal; 70. Slide holder; 702. Bottom opening; 71. Support plate; 72. Insertion slot; 81. First temperature sensor; 82. Second temperature sensor; 83. Pressure relief valve; 84. Pressure relief pipe; 90. Housing; 900. Slide glass. Detailed implementation mode

[0048] To make the technical problems solved by the present utility model, the technical solutions adopted and the achieved technical effects clearer, the technical solutions of the embodiments of the present utility model will be further described in detail below with reference to the drawings. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without making creative efforts belong to the scope of protection of the present utility model.

[0049] In the description of the present utility model, unless otherwise clearly defined and limited, the terms "connected" and "fixed" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection or integrated; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the internal connection of two components or the interaction relationship between two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations.

[0050] In the present utility model, unless otherwise clearly specified and defined, the first feature being "above" or "below" the second feature may include the direct contact between the first and second features, or may include the situation where the first and second features are not in direct contact but in contact through additional features therebetween. Moreover, the first feature being "above", "over" and "on top of" the second feature includes that the first feature is directly above and obliquely above the second feature, or merely indicates that the horizontal height of the first feature is higher than that of the second feature. The first feature being "below", "beneath" and "underneath" the second feature includes that the first feature is directly below and obliquely below the second feature, or merely indicates that the horizontal height of the first feature is lower than that of the second feature.

[0051] Immunohistochemistry, also known as immunohistochemical technique (IHC) in full, is a technique that applies the principle of specific binding between antigens and antibodies. By means of a chemical reaction, the chromogenic agent (fluorescein, enzyme, metal ion, isotope) labeled with an antibody is made to develop color to determine the antigens (polypeptides and proteins) in tissue cells, so as to conduct research on their localization, qualitative analysis and quantitative analysis. Immunohistochemistry is a commonly used method in clinical pathological examination and experimental pathology. During the immunohistochemistry operation process, antigen retrieval technology is always involved. Generally, antigen retrieval technology involves soaking the sections in an antigen retrieval solution, heating the sections for a period of time, and then performing subsequent operations to obtain a better staining effect. The purpose of performing antigen retrieval (AR) is to break the cross-linking between antigen proteins and formaldehyde in the tissue and the interconnections between proteins, fully expose the tissue antigens, so that most antibodies can effectively bind to the antigens and specific immunostaining can occur, thereby improving the detection rate of tissue antigens.

[0052] In the related art, in immunohistochemistry technology, the antigen retrieval efficiency is relatively slow. With the increasing annual incidence of tumors, the annual growth rate of immunohistochemical staining experiments related to tumor diagnosis has been gradually increasing, which has led to a growing workload and working intensity of immunohistochemistry experiments. The low antigen retrieval efficiency will result in a relatively long time for the entire immunohistochemistry experiment, failing to meet the requirements.

[0053] In the antigen retrieval methods in the related art, the tissue samples on the glass slides are heated by methods such as hot plate heating and water bath heating. However, this heating method can at most heat the retrieval solution to 100 degrees, so the entire antigen retrieval process takes a relatively long time, about 20 to 30 minutes, with low efficiency and limited retrieval effect. In some laboratories, a pressure cooker is additionally configured. The glass slides carrying tissue sections are placed in the pressure cooker to perform high-temperature water bath antigen retrieval inside the pressure cooker. However, the pressure cooker must be manually operated by the operator. However, when the workload of immunohistochemistry experiments is large, the manual operation efficiency is low.

[0054] Based on this, the present application provides a cross-type lifting power module and an antigen repair device. The antigen repair device can form a high-pressure and high-temperature environment in the inner container to shorten the antigen repair time and improve the antigen repair effect.

[0055] The cross-type lifting power module is applied in the antigen repair device and can drive the gland to seal or open the inner container. Moreover, by using a driving mechanism to drive the cross frame to open or close, so as to drive the gland to move, a more uniform acting force can be applied to the gland, making the movement stroke of the gland more stable and enabling the gland to seal the first opening of the inner container more accurately. In other words, the cross-type lifting power module can be applied to the heating device for immunohistochemical staining (IHC staining). In other embodiments, the cross-type lifting power module can also be applied to the heating device for fluorescence in situ hybridization staining (FISH staining).

[0056] The present application also provides an immunohistochemical device, including the aforementioned antigen repair device. The immunohistochemical device has high efficiency. In some embodiments, the immunohistochemical device includes a manipulator, which is used to send a slide carrying a tissue sample into the antigen repair device or take it out from the antigen repair device, realizing the automated operation of some processes. The immunohistochemical device can at least automatically or semi-automatically perform the antigen repair step, and can also perform one or more of the steps such as baking the slice, dewaxing, hydration, primary antibody incubation, secondary antibody incubation, color development, counterstaining, dehydration, and clearing.

[0057] Please refer to Figures 1 to 14 as follows, and the antigen repair device of the present application will be described below. The antigen repair device includes a housing 90, an inner container 10, a heating assembly 50, a first seal 61, and a cross-type lifting power module.

[0058] Please refer to Figure 3 , Figure 4 , Figures 9 to 14 As shown in, an inner water tank 101 is arranged inside the inner container 10, and the inner water tank 101 is used to hold the repair liquid; a first opening 102 is arranged on one side of the inner container 10, and the first opening 102 is communicated with the inner water tank 101. The slide 900 can be placed into the inner water tank 101 through the first opening 102, so that the repair liquid can soak the tissue sample on the slide 900. The heating assembly 50 is arranged inside or outside the inner container 10, and the heating assembly 50 is used to heat the liquid in the inner water tank 101.

[0059] Please refer to Figures 2 to 8The cross-type lifting power module includes a driving mechanism 41, a lifting mechanism and a cross frame 42. The fixed end of the driving mechanism 41 is installed on the shell 90, and the output end is connected to the cross frame 42 in a transmission connection. The cross frame 42 is connected to the lifting mechanism in a transmission connection, and the lifting mechanism includes a pressure cover 30, which is used to seal the first opening 102 of the inner container 10. The cross frame 42 is also called a scissors frame, etc. The cross frame 42 includes two cross-arranged and mutually hinged brackets 421. The driving mechanism 41 is used to drive the two brackets 421 in the cross frame 42 to open or close, so as to drive the lifting mechanism to move through the movement of the ends of the brackets 421 of the cross frame 42, thereby driving the pressure cover 30 to move.

[0060] The pressure cover 30 has a first position and a second position. The first position of the pressure cover 30 is also called the open position, and the second position of the pressure cover 30 is also called the closed position. The driving mechanism 41 drives the cross frame 42 to move, and the cross frame 42 drives the pressure cover 30 to move between the first position and the second position. Figure 1 , Figures 2 to 4 , Figure 9 As shown, the pressure cover 30 is in the first position, at which time the pressure cover 30 is separated from the inner container 10, the first opening 102 is open, and the inner water tank 101 is not closed. Figures 11 to 13 As shown, when the pressure cover 30 is in the second position, the pressure cover 30 covers the first opening 102, and the first sealing member 61 is clamped between the pressure cover 30 and the inner container 10. The first sealing member 61 seals the gap between the pressure cover 30 and the inner container 10 to enclose a heating chamber between the inner wall of the inner container 10 and the pressure cover 30.

[0061] The antigen repair device further includes a heating component 50, which is used to heat the reagent in the inner water tank 101 to increase the temperature of the reagent. The heating component 50 can be arranged outside the inner water tank 101 and not in contact with the liquid in the inner container 10; the heating component 50 can also be arranged inside the inner water tank 101, for example, embedded in the plate body of the inner container 10, or arranged outside the inner container 10.

[0062] The antigen repair device of the present application can be used in immunohistochemistry experiments. The following provides an antigen repair method based on the antigen repair device of the present application.

[0063] Add the reagents needed for antigen repair into the inner water tank 101, which may be but not limited to EDTA antigen repair solution; send the glass slide 900 carrying the target tissue sample into the inner water tank 101, and drive the pressure cover 30 to move to the second position through the lifting power module, so that the pressure cover 30, the first sealing member 61, and the inner container 10 are in close contact to seal the first opening 102 of the inner container 10; the heating component 50 is operated to heat the reagent in the inner container 10.

[0064] In immunohistochemical staining experiments, after tissues are fixed with formalin, proteins crosslink and antigens are blocked. In the antigen retrieval step, reagents such as antigen retrieval buffer can, under heated conditions, re-expose antigens shielded by formalin without damaging antigen epitopes, thereby increasing the detection rate of antigens, reducing background staining, and improving the diagnostic accuracy.

[0065] The antigen retrieval device of the present application and the immunohistochemical equipment using the antigen retrieval device have at least the following effects:

[0066] First, in the process of antigen retrieval, under the action of the lifting power module, the pressing cover 30 always remains in the second position closing the first opening 102. At this time, the interior of the inner container 10 is a sealed space. Under the action of the heating component 50, the reagent gradually heats up. As the temperature of the reagent rises, water vapor is gradually generated and cannot escape, thereby increasing the pressure inside the inner container 10 to achieve high-pressure heating or near high-pressure heating. Compared with the conventional water bath heating method and microwave heating method that can reach 98 degrees Celsius and 100 degrees Celsius, the high-pressure heating method can make the reagent reach a higher temperature, about 120 degrees Celsius. High-temperature retrieval has higher efficiency. In the case of achieving the same antigen retrieval effect, the antigen retrieval time required by the high-pressure heating method is shorter. Exemplarily, if a glass slide 900 carrying tissues is placed in a water bath box for retrieval, it generally takes 20 minutes of water bath at 100 degrees Celsius, while if the antigen retrieval device of the present application is used for retrieval in a high-pressure environment at 120 degrees for 2 to 8 minutes, the antigen retrieval time is saved.

[0067] Third, the inner container 10 can accommodate multiple glass slides 900, and multiple tissue samples can be retrieved simultaneously, which can increase the number of tissue samples processed by the immunohistochemical equipment in one staining process, that is, improve the throughput of the equipment, and can improve the experimental efficiency.

[0068] Fourth, during the high-pressure heating process, the reagent and tissue samples are heated more evenly, reducing experimental errors; under high-pressure conditions, the antigen retrieval solution can penetrate more fully into the tissue sections, helping to reduce antigen loss and improve the accuracy of experimental results.

[0069] Fifth, the antigen retrieval device is conducive to being applied in fully automatic or semi-automatic immunohistochemical equipment. The closing and opening of the pressing cover 30 do not require manual operation, and only need to control the lifting power module through the controller, thereby improving the experimental efficiency of immunohistochemical experiments, shortening the experimental time-consuming, and reducing manual operation errors.

[0070] In the antigen repair device of the present application, when performing antigen repair, it is important that the pressure cover 30 accurately covers the first opening 102 of the inner container 10 and the inner container 10 is well sealed. In order to shorten the antigen repair time and improve the antigen repair effect, a cross frame 42 is configured in the lifting power module of the present application, and the lifting power module is described in detail below.

[0071] Reference Figures 2 to 4 , Figure 14 , the cross frame 42 includes two brackets 421. Figure 2 , Figure 8 As shown in the figure, the two ends of the bracket 421 are the first bracket end 422 and the second bracket end 423. The bracket 421 includes a hinged portion 424 located between the first bracket end 422 and the second bracket end 423. The hinged portions 424 of the two brackets 421 are hinged. The second bracket ends 423 of the two brackets 421 in the cross frame 42 are both connected to the lifting mechanism in a transmission manner, that is, the two second bracket ends 423 in the cross frame 42 are used to drive the pressure cover 30 to move. Among them, the output end of the driving mechanism 41 is connected to the first bracket end 422 of the bracket 421 in a transmission manner, and the driving mechanism 41 is used to drive the two brackets 421 in the cross frame 42 to open or close, so as to drive the pressure cover 30 to rise or fall.

[0072] When the gland 30 is required to press the inner container 10 to seal the inner container 10, the cross frame 42 applies uniform pressure to the gland 30, which can compact the gland 30 in the inner container 10, and the gland 30 has a better sealing effect on the first opening 102, and it is not easy to release pressure from the gland 30, which is conducive to providing a high-pressure heating environment. In addition, the cross frame 42 is used to drive the gland 30 to rise and fall, and the lifting stroke of the gland 30 is more stable, so that the gland 30 can accurately seal the first opening 102 and ensure the sealing of the inner container 10.

[0073] In one embodiment, the lifting power module is configured to have a self-locking capability. After the pressure cover 30 reaches the second position, the lifting power module uses its self-locking force to keep the pressure cover 30 in the second position, which is beneficial to maintaining the pressure inside the inner container 10, so that the reagents inside the inner container 10 and the tissue samples on the slide 900 can be heated under a high pressure environment, and there is no need to specially add a locking step to lock the pressure cover 30 in the second position, and the entire heating operation process is simpler and more reliable.

[0074] The pressure cover 30 can move along the z1 direction under the drive of the lifting power module. Figure 3 The first position shown in FIG. 1 moves to Figure 11 The second position shown in the figure, continue to refer to Figure 11, when the gland 30 is in the second position, the gland 30 seals the first opening 102 of the inner container 10, and a closed space is formed inside the inner container 10. As the heating process progresses, the pressure inside the inner container 10 increases, and there will be a gas pressure in the z2 direction opposite to the z1 direction acting on the gland 30. In this embodiment, a lifting power module with self-locking ability is adopted, which can overcome the action of the gas pressure in the z2 direction and keep the gland 30 in the second position.

[0075] Optionally, the driving mechanism 41 is a motor that rotates bidirectionally. When the motor rotates forward and backward, it drives the gland 30 to move to the first position and the second position respectively.

[0076] In one embodiment, a transmission assembly is provided between the driving mechanism 41 and the cross frame 42. A transmission assembly with self-locking ability is adopted. The transmission assembly includes a first transmission member and a second transmission member (the two transmission members are not shown in the figure). The output end of the driving mechanism 41 is in transmission connection with the first transmission member, the first transmission member is in transmission connection with the second transmission member, and the second transmission member is in transmission connection with the cross frame 42. The transmission assembly having self-locking ability means that when the first transmission member moves, it can drive the second transmission member to move, but conversely, the second transmission member cannot drive the first transmission member to move.

[0077] Optionally, the first transmission member is a worm and the second transmission member is a worm wheel. The output end of the driving mechanism 41 is in transmission connection with the worm, the worm is in transmission connection with the worm wheel, and the worm wheel is in transmission connection with the first frame end 422 of the bracket 421. Among them, the worm can drive the worm wheel to rotate, but during normal transmission, the worm wheel cannot drive the worm to rotate. This is the result of the combined action of factors such as the meshing relationship, transmission ratio, and friction angle between the worm and the worm wheel. The principle of the self-locking ability of the worm and the worm wheel will not be elaborated here.

[0078] It can be understood that the combination of the worm and the worm wheel can also be used as a speed reduction assembly, thereby increasing the torque output from the driving mechanism 41 to the cross frame 42. Of course, in other embodiments, the first transmission member and the second transmission member can also be other transmission structures.

[0079] In one embodiment, the driving mechanism 41 is a stepper motor, and the motor shaft of the stepper motor is the output end. The stepper motor can rotate precisely according to a predetermined angle or step size. By controlling the number of pulses input to the stepper motor, the opening or closing degree of the cross frame 42 can be precisely controlled, thereby driving the moving distance of the gland 30 and precisely controlling the gland 30 to reach the second position, ensuring the sealing and heating effect of the inner container 10. In addition, the stepper motor also has self-locking ability and can stay in the current position under the action of electromagnetic force.

[0080] In other embodiments, a worm and a worm gear may not be provided between the driving mechanism 41 and the cross frame 42, and the driving mechanism 41 may not be a stepping motor but other rotary driving mechanisms 41.

[0081] In other embodiments, in order to keep the gland 30 in the second position during heating, a lifting power module with self-locking ability may not be adopted, but a locking member may be additionally provided. After the gland 30 reaches the second position, the gland 30 is locked to the inner container 10 by means of a locking member through control by a controller or manual locking by a human.

[0082] Please continue to refer to FIGS. 2 to Figure 8 , in one embodiment, it includes two cross frames 42 which are arranged at intervals. The two cross frames 42 are each configured such that the first frame end 422 of at least one support 421 is in transmission connection with the output end of the driving mechanism 41, and one or two second frame ends 423 of each cross frame 42 are in transmission connection with the gland 30. In this way, the driving mechanism 41 can drive the two cross frames 42 to open or close simultaneously, transmit force to the gland 30 through different cross frames 42 simultaneously, and drive the gland 30 to rise or fall through the two cross frames 42 simultaneously, so as to improve the stability of the movement of the gland 30. Moreover, when the lifting power module has a self-locking force, when sealing and heating the inner container 10, the air pressure inside the inner container 10 can act on the two cross frames 42 on average, and the two cross frames 42 can be used simultaneously to keep the gland 30 in the second position of sealing the inner container 10, and the sealing effect is good.

[0083] When the four first frame ends 422 of the two cross frames 42 are all in transmission connection with the driving mechanism 41, the driving mechanism 41 can stably drive the two cross frames 42 to open or close. When the four second frame ends 423 of the two cross frames 42 are all in transmission connection with the gland 30, the driving force can be transmitted to the gland 30 through the four second frame ends 423 simultaneously, and the movement of the gland 30 is more stable and is more stably kept in the second position.

[0084] Wherein, the driving mechanism 41 can drive the gland 30 to reciprocate along the z direction through the cross frame 42. The two cross frames 42 are arranged at intervals along the x direction, and the two second frame ends 423 in each cross frame 42 are distributed at intervals along the y direction. The x direction, the y direction and the z direction are perpendicular to each other in pairs. Exemplarily, the z direction is the up and down direction, the y direction is the front and back direction, and the x direction is the left and right direction.

[0085] In other embodiments, the number of cross frames 42 may also be one, three or more than three.

[0086] Please refer to Figures 1 to 5 , Figure 8, the lifting mechanism includes a transmission seat 43. The gland 30 is connected to the transmission seat 43, and the gland 30 is disposed on the side of the transmission seat 43 close to the inner container 10. The second end portion 423 of each bracket 421 is in transmission connection with the transmission seat 43.

[0087] In one embodiment, in the case of including two cross brackets 42 and a transmission seat 43, the transmission seat 43 is provided with a sliding groove 431, and the cross-type lifting power module further includes two sliding rods 442. There are two sliding rods 442 and two cross brackets 42. The first second end portion 423 of the first cross bracket 42 is connected to the first second end portion 423 of the second cross bracket 42 through at least one sliding rod 442; the second second end portion 423 of the second cross bracket 42 is connected to the second second end portion 423 of the second cross bracket 42 through at least one sliding rod 442; the sliding rods 442 are slidably mounted in the sliding groove 431.

[0088] Exemplarily, two cross brackets 42 are correspondingly provided with two sliding rods 442. When the driving mechanism 41 drives the two cross brackets 42 to open simultaneously, the two sliding rods 442 move away from each other, and the two sliding rods 442 simultaneously apply a driving force to the transmission seat 43 to move in a direction away from the inner container 10, and the transmission seat 43 drives the gland 30 to move to the first position. When the driving mechanism 41 drives the two cross brackets 42 to close simultaneously, the two sliding rods 442 approach each other, and the two sliding rods 442 simultaneously apply a driving force to the transmission seat 43 to move in a direction close to the inner container 10, and the transmission seat 43 drives the gland 30 to move to the second position. As Figure 3 , Figure 5 , Figure 8 shown, the transmission seat 43 is provided with two transmission grooves, the two transmission grooves are arranged at intervals along the y direction, and both ends of each transmission groove penetrate in the x direction. The two sliding rods 442 are respectively inserted into the two transmission grooves.

[0089] In this embodiment, by providing two sliding rods 442, on the one hand, the synchronism of the movement of the two cross brackets 42 can be improved to uniformly apply a driving force to the transmission seat 43 and the gland 30. On the second hand, the four second end portions 423 in the two cross brackets 42 can apply an upward or downward driving force to the transmission seat 43 by means of the two sliding rods 442. The contact area between the sliding rods 442 and the transmission seat 43 is relatively large. Compared with the scheme in which the four second end portions 423 are independently slidably mounted on the transmission seat 43 respectively, this embodiment can apply a driving force to the transmission seat 43 and the gland 30 more uniformly. In this way, when it is necessary to drive the gland 30 to press on the inner container 10 to seal the second opening 202, the gland 30 is uniformly stressed and has good sealing performance for the inner container 10.

[0090] In other embodiments, the transmission base 43 may also be provided with only one transmission slot, and the two sliding rods 442 are both slidably mounted in the same transmission slot. In other embodiments, the sliding rods 442 may not be provided, and the four second frame ends 423 are independently mounted on the transmission base 43 respectively.

[0091] Please continue to refer to Figures 3 to 5 、 Figure 8 , in one embodiment, the lifting power module is configured as follows: the driving mechanism 41 is used to drive the two first frame ends 422 in the cross frame 42 to approach or move away from each other, so as to drive the cross frame 42 to close or open. The lifting power module further includes a housing 90 and a lead screw 451 assembly. The housing 90 is fixed, and the lead screw 451 assembly includes a lead screw 451, a first bushing 452 and a second bushing 453. The first end of the lead screw 451 is in transmission connection with the driving mechanism 41, and the second end of the lead screw 451 is rotatably connected to the housing 90. The lead screw 451 is provided with a first rod section and a second rod section with opposite spiral directions. The first bushing 452 is threadedly connected to the first rod section, and the second bushing 453 is threadedly connected to the second rod section. In the same cross frame 42, one first frame end 422 is connected to the first bushing 452, and the other first frame end 422 is connected to the second bushing 453. When the lead screw 451 rotates, the first bushing 452 and the second bushing 453 approach or move away from each other, so as to drive the two first frame ends 422 in the same cross frame 42 to approach or move away from each other, and further drive the cross frame 42 to close or open, thereby driving the gland 30 to descend or ascend. Through the arrangement of the lead screw 451 assembly, the driving mechanism 41 applies driving forces to both of the two first frame ends 422 in the cross frame 42. In this way, the driving mechanism 41 can transmit power to the cross frame 42 more evenly and stably, so as to drive the gland 30 to move more evenly and stably.

[0092] In other embodiments, the lead screw 451 may also be provided with threads in only a single rotation direction, and only one bushing is correspondingly provided. Only one first frame end 422 in the cross frame 42 is connected to the bushing, and the other frame end is slidably mounted on the housing 90.

[0093] In one embodiment, the lifting power module further includes a reduction gearbox 47. The output end of the driving mechanism 41 is in transmission connection with the input end of the reduction gearbox 47, and the output end of the reduction gearbox 47 is in transmission connection with the first end of the lead screw 451. Taking the driving mechanism 41 as a motor as an example, a reduction gearbox 47 is arranged between the motor and the lead screw 451, which can reduce the rotational speed output to the lead screw 451 and increase the torque output to the lead screw 451, so as to drive the lead screw 451 to rotate more stably and labor-savingly, with high reliability.

[0094] In one embodiment, the lifting power module further includes a housing 90, a horizontal guide rail 461 mounted on the housing 90, and a vertical guide rail 462 mounted on the housing 90. One or both of the first frame ends 422 of the two brackets 421 of the cross frame 42 are slidably connected to the horizontal guide rail 461. When the driving mechanism 41 drives the cross frame 42 to open or close, the first frame end 422 slides along the horizontal guide rail 461. At least one hinge portion 424 of the cross frame 42 is slidably connected to the vertical guide rail 462 through a connecting shaft 441. When the driving mechanism 41 drives the cross frame 42 to open or close, the connecting shaft 441 slides along the vertical guide rail 462. By providing the horizontal guide rail 461 and the vertical guide rail 462, the movement of the cross frame 42 can be made more stable, avoiding the shaking of the cross frame 42, and further avoiding the shaking of the gland 30, ensuring that the edge of the gland 30 can accurately press against the upper edge of the inner container 10 when the gland 30 is pressed down, so as to ensure that the gland 30 can completely cover the first opening 102 on the upper side of the inner container 10 and ensure good sealing of the inner container 10 after the gland 30 is pressed down.

[0095] In other embodiments, only the horizontal guide rail 461 or only the vertical guide rail 462 may be provided.

[0096] Optionally, in one embodiment, referring to Figure 2 , Figures 4 to 8 , the cross-type lifting power module is provided with two vertical guide rails 462. The hinge portions 424 of the two brackets 421 in the cross frame 42 are rotatably mounted on the connecting shaft 441. The two ends of the connecting shaft 441 are respectively slidably connected to the two vertical guide rails 462. Through one shaft, the hinge between the brackets 421 of the two cross frames 42 is realized, and the two cross frames 42 are also slidably mounted along the vertical direction on the two side vertical guide rails 462. The structure is compact, the integrity of the two cross frames 42 is strong, the opening and closing stroke of the cross frame 42 is stable, and the rising and falling stroke of the gland 30 is stable, so as to ensure that the gland 30 accurately seals the first opening 102 of the inner container 10.

[0097] The following will describe in detail other structures in the antigen repair device.

[0098] In one embodiment, a first annular groove is provided on the side of the gland 30 close to the inner container 10. The first seal 61 is an O-ring, and the first seal 61 is disposed in the first annular groove. The first seal 61 is fixed to the gland 30 by means such as adhesion or interference fit. The area of the gland 30 is larger than the area of the first opening 102. When the gland 30 is in the second position, the outer edge region of the gland 30 presses the first seal 61, the first seal 61 presses the top end face of the inner container 10, and the central region of the gland 30 closes the first opening 102. At this time, the lifting power module applies a pressure F towards the top end face of the inner container 10 to the gland 30, so that the gland 30 not only keeps sealing the first opening 102, but also continuously presses the first seal 61. During heating, a good sealing effect on the first opening 102 is achieved, which is beneficial to the increase of the internal pressure of the inner container 10. It can be understood that the setting of the first annular groove can limit the position of the first seal 61, so that the first seal 61 deforms under the pressure of the gland 30 but does not shift, and the first seal 61 is kept between the gland 30 and the top end of the inner container 10.

[0099] Please refer to Figure 9 、 Figure 12 , the gland 30 includes a gland body 31 and a first boss 32 provided on the side of the gland body 31 close to the inner container 10. When the gland 30 is in the second position and antigen repair heating is performed, the first seal 61 is clamped between the gland body 31 and the inner container 10, and the first boss 32 is inserted into the interior of the inner container 10. The first boss 32 is used to limit the lateral movement of the gland 30 to prevent the gland 30 from shifting horizontally under the action of the internal pressure, ensuring that the gland body 31 can stably cover the top end of the inner container 10 to avoid seal failure and ensure the effect of high-pressure heating. During the process of the gland 30 covering the first opening 102 of the inner container 10, the first boss 32 also plays a guiding role.

[0100] Optionally, the first annular groove is provided on the gland body 31 and the first annular groove is distributed around the first boss 32, thereby enhancing the pressing and sealing effect of the gland 30.

[0101] In other embodiments, an annular groove may also be provided at the top end of the inner container 10 to accommodate the first seal 61.

[0102] Please continue to refer to Figures 1 to 4 、 Figure 9 , in one embodiment, the antigen repair device further includes a slide holder 70, and the slide holder 70 is used to carry the slide 900. One or more mounting portions are provided on the slide holder 70, and each mounting portion is used to carry the slide 900.

[0103] The slide holder 70 is disposed on the side of the gland 30 close to the inner container 10, and the side of the slide 900 holder facing away from the inner container 10 is connected to the gland 30. When the lifting power module drives the gland 30 to move away from or close to the inner container 10, the slide holder 70 is driven by the gland 30 to move away from or close to the inner container 10. Exemplarily, the slide holder 70 is fixed to the lower side of the gland 30. When antigen repair is required, the lifting power module drives the gland 30 and the slide holder 70 to move downward. Referring to Figure 11 , under the downward force applied by the lifting power module, the gland 30 keeps pressing downward on the inner container 10 with good sealing, and the slide holder 70 remains inside the inner container 10.

[0104] Referring to Figure 1 , Figure 3 , Figure 4 , 9 , when the gland 30 is in the first position, the gland 30 is separated from the inner container 10, and the slide holder 70 is located outside the inner container 10, avoiding the inner container 10 from blocking the slide holder 70. By means of automatic operation of the manipulator or manual operation of the experimenter, the slide 900 carrying the tissue sample to be repaired can be placed on the mounting part of the slide holder 70, or the slide 900 carrying the tissue sample that has completed antigen repair can be taken out from the slide holder 70.

[0105] Referring to Figure 11 , when the gland 30 is in the second position, the gland 30 seals the first opening 102, and the slide holder 70 is located in the inner water tank 101. The slide 900 in the slide holder 70 and the tissue sample on the slide 900 are also in the inner water tank. In this way, the reagent in the inner water tank 101 can soak the tissue sample on the slide 900.

[0106] Based on the configuration in this embodiment where the gland 30 fixes the slide holder 70, with the same set of lifting power module, the gland 30 and the slide holder 70 can be simultaneously driven to approach the inner container 10. When the gland 30 reaches the second position, two experimental operations of feeding the slide 900 into the inner water tank 101 and pressing the gland 30 tightly against the inner container 10 can be simultaneously achieved. Similarly, with the same set of lifting power module, the gland 30 and the slide holder 70 can be driven to leave the inner container 10 simultaneously to facilitate opening the inner container 10 and taking out the slide 900 carrying the tissue sample that has completed antigen repair. In other words, one drive of the lifting power module can simultaneously achieve two operations, with higher efficiency, time saving, and no need for manual feeding of the slide 900 into the inner container 10, which is suitable for realizing automated operation.

[0107] Please refer to Figure 9, in an embodiment, a bottom opening 702 is provided on the side of the specimen holder 70 facing away from the gland 30. When there is reagent in the inner water tank 101, during the process of the specimen holder 70 entering the inner water tank 101, the reagent can enter the specimen holder 70 through the bottom opening 702, thereby wetting the tissue sample on the specimen holder 70.

[0108] In an embodiment, lateral openings are provided at one end or opposite ends of the specimen holder 70. The lateral openings and the bottom opening 702 are located on adjacent sides of the specimen holder 70. At least one lateral opening serves as an access channel for the glass slide 900. In the immunohistochemistry device, the robotic arm can send the glass slide 900 into or take it out of the specimen holder 70 through the lateral opening of the specimen holder 70, improving efficiency. The lateral openings on the side of the specimen holder 70 are also beneficial for the reagent to enter the interior of the specimen holder 70 to wet the tissue sample on the specimen holder 70. When lateral openings are provided at both opposite ends of the specimen holder 70 and the extending direction of the bearing surface of the glass slide 900 also extends from one lateral opening to the other lateral opening, when the reagent in the inner container 10 is heated, the thermal movement of the reagent is enhanced, and the reagent can flow from one lateral opening of the specimen holder 70 to the other lateral opening. The reagent flow direction is as Figure 8 schematically indicated by the solid arrow in. In this way, the reagent can flow over the surface of the glass slide 900, thereby flowing through the tissue sample. By wetting the tissue sample with the heated reagent, antigen retrieval can be made more uniform and the antigen retrieval time can be shortened.

[0109] Optionally, the specimen holder 70 includes two support plates 71 which are spaced apart. Mounting portions are provided on the side of any one support plate close to the other support plate 71. The mounting portions are insertion slots 72. The glass slide 900 can be inserted between the two support plates 71 from the lateral opening. The two sides in the width direction of the glass slide 900 are respectively inserted into the insertion slots 72 of the two side support plates 71, and the specimen holder 70 supports the glass slide 900.

[0110] Optionally, the antigen retrieval device includes a housing 90. The inner container 10, the heating assembly 50, the cross-shaped lifting power module, and the specimen holder 70 are all arranged inside the housing 90. The housing 90 is provided with a housing opening. At least one lateral opening of the specimen holder 70 corresponds to the position of the housing opening of the housing 90. When the robotic arm sends the glass slide 900, the glass slide 900 is sent into the specimen holder 70 from the housing opening and the lateral opening.

[0111] Referring to Figure 9 , in an embodiment, the gland 30 is provided on the upper side of the specimen holder 70, and the first opening 102 is located on the upper side of the inner container 10. Lateral openings are provided on both the front side and the rear side of the specimen holder 70. When the gland 30 is in the second position, the glass slide 900 is horizontally placed in the specimen holder 70 and is horizontally located in the inner container 10. In this way, as Figure 3As shown, it is convenient for the manipulator to send the slide holder 70 into or take out the slide holder 70 in the horizontal direction.

[0112] It should be noted that the slide holder 70 can also be of other structures, as long as it can carry and support the glass slide 900 and can actually infiltrate the tissue sample on the surface of the glass slide 900.

[0113] In an embodiment, the antigen retrieval device further includes an outer container 20. The interior of the outer container 20 has an outer water tank 201. A second opening 202 is provided on one side of the outer container 20, and the second opening 202 communicates with the outer water tank 201. The inner container 10 is disposed inside the outer container 20. The first opening 102 is located on the upper side of the inner container 10, and the second opening 202 is located on the upper side of the outer container 20. The inner container 10 is provided with a communication port. When the pressing cover 30 is in the first position, the inner water tank 101 communicates with the outer water tank 201 through the communication port, and the reagent in the outer water tank 201 can enter the inner water tank 101. When the pressing cover 30 is in the second position, the pressing cover 30 seals the communication port, and a heating chamber is formed by enclosing between the pressing cover 30 and the inner container 10. The space inside the inner container 10 is sealed and can be heated under high pressure.

[0114] In an embodiment, when the pressing cover 30 is in the first position, the height of the first opening 102 of the inner container 10 is lower than the height of the second opening 202 of the outer container 20, that is, the first opening 102 is farther from the pressing cover 30 relative to the second opening 202. The aforementioned communication port is the first opening 102 of the inner container 10. Of course, in other embodiments, when the inner container 10 includes an inner chamber body 11 and an inner bottom plate 12, the communication port can also be located between the inner chamber body 11 and the inner bottom plate 12.

[0115] Optionally, the outer container 20 is provided with a liquid pipe, which is connected to an external liquid supply device through the liquid pipe, so that the reagent can be automatically added to the outer water tank 201, or the reagent in the outer water tank 201 can be discharged through the liquid pipe. Due to the existence of the communication port between the inner water tank 101 and the outer water tank 201, the reagent can be injected into the inner container 10 through the outer container 20, or the reagent in the inner container 10 can be discharged to the outer container 20 without configuring a separate liquid pipe in the inner container 10. The liquid inlet, liquid discharge, heating, cooling, and cleaning of the inner container 10 and the outer container 20 all use the same water path and are all connected to the outside of the antigen retrieval device through the same liquid pipe provided on the outer container 20. When the antigen retrieval device is applied to an immunohistochemical staining device, the assembly of the antigen retrieval device and other devices in the staining machine is simple, and the control of the antigen retrieval device is also simple.

[0116] In one embodiment, three separate components cooperate to enclose and form a sealed heating chamber. In this embodiment, the inner container 10 includes an inner chamber body 11 and an inner bottom plate 12. The inner chamber body 11 is movably connected to the inner bottom plate 12, and a second seal 62 is provided on the inner chamber body 11 and / or the inner bottom plate 12. The second seal 62 can be, but is not limited to, an O-ring.

[0117] Optionally, when the lifting power module drives the pressing cover 30 to move downward to the second position, the first seal 61 is clamped between the pressing cover 30 and the inner chamber body 11, and the second seal 62 is clamped between the inner chamber body 11 and the inner bottom plate 12. A heating chamber is enclosed among the pressing cover 30, the inner chamber body 11, and the inner bottom plate 12. Due to the presence of the first seal 61 and the second seal 62, when the pressing cover 30 is held at the second position under the action of pressure, a sealed heating chamber is formed inside the inner container 10. The liquid in the heating chamber is continuously heated by the heating assembly 50 to continuously increase in temperature. The gas in the heating chamber cannot or is difficult to overflow, and a high-pressure environment can be formed in the heating chamber. The tissue sample on the glass slide 900 can be antigen repaired in the high-pressure heating environment, shortening the repair time and improving the repair effect.

[0118] Optionally, when the pressing cover 30 is in the second position, the first seal 61 is located below the pressing cover 30 and above the inner chamber body 11, and the second seal 62 is located below the inner chamber body 11 and above the inner bottom plate 12. As Figures 11 to 13 shown, when the lifting power module applies a downward pressure to keep the pressing cover 30 sealingly covering the first opening 102 at the top of the inner container 10 against the action of the internal steam, the pressing cover 30 presses the first seal 61 downward, and the inner chamber body 11 presses the second seal 62 downward. Both the upper and lower seals can be deformed under the action of the downward pressure. Exemplarily, after the first seal 61 is deformed, it can better fit the pressing cover 30 and the inner chamber body 11, thereby improving the sealing effect. Of course, in other embodiments, when the pressing cover 30 is in the second position, the first seal 61 can also be clamped between the outer wall of the inner chamber body 11 and the pressing cover 30, and the second seal 62 can also be clamped between the outer wall of the inner chamber body 11 and the inner bottom plate 12.

[0119] Referring to Figure 9 、 Figure 14 , the inner chamber body 11 is vertically through, the first opening 102 is formed at the top of the inner chamber body 11, the inner bottom plate 12 is provided at the bottom of the inner bottom plate 12, and an inner water tank 101 is enclosed between the surface of the inner chamber body 11 and the surface of the inner bottom plate 12.

[0120] In one embodiment, the inner chamber body 11 is slidably connected to the inner bottom plate 12, and the inner chamber body 11 can move up and down relative to the inner bottom plate 12. An elastic member (not shown in the figure) is provided between the inner chamber body 11 and the inner bottom plate 12, and the elastic force of the elastic member acts on the inner chamber body 11 to make the inner chamber body 11 tend to move upward.

[0121] As Figure 9 , Figure 10 , Figure 14 shown, when the gland 30 is in the first position, a gap is formed between the inner cavity body 11 and the inner bottom plate 12 to form a liquid channel 13, and the inner water tank 101 is communicated with the outer water tank 201 through the liquid channel 13. As Figures 8 to 10 shown, when the gland 30 is in the second position, the inner cavity body 11 abuts against the second seal 62, the second seal 62 abuts against the inner bottom plate 12, and the liquid channel 13 is closed.

[0122] Among them, the second seal 62 can be relatively fixedly arranged at the bottom of the inner cavity body 11. In this way, when the gland 30 is in the first position, the second seal 62 and the inner bottom plate 12 are spaced apart from each other and do not contact, and a liquid discharge micro-channel is formed between the second seal 62 and the inner bottom plate 12. Of course, the second seal 62 can also be relatively fixedly arranged on the top of the inner bottom plate 12. In this way, when the gland 30 is in the first position, the inner cavity body 11 and the second seal 62 are spaced apart from each other and do not contact. As Figure 10 , Figure 14 schematic, the liquid channel 13 is formed between the inner cavity body 11 and the second seal 62.

[0123] With the configuration of the liquid channel 13, when the liquid needs to be discharged from the liquid channel 13, the liquid can be discharged to the outer water tank 201 of the outer container 20, so that the outer container 20 can be used to meet the liquid discharge requirement of the inner container 10, and there is no need to specially arrange a liquid discharge pipe in the inner container 10, and the structure is simple. Exemplarily, after antigen repair is completed, first open the pressure relief valve 83 to relieve the pressure inside the inner container 10. After the pressure relief is completed, the lifting power module drives the gland 30 to rise, the slide 900 is taken out, the gland 30 leaves the second position and resets to the first position, and a liquid channel 13 is formed between the inner cavity body 11 and the inner bottom plate 12. The heated repair liquid inside the inner container 10 can be discharged to the outer water tank 201 of the outer container 20 through the liquid channel 13 at the bottom, and the liquid in the outer container 20 is discharged through the pipeline.

[0124] It can be understood that by splitting the inner container 10 into two components, namely the inner cavity body 11 and the inner bottom plate 12, and arranging the second seal 62 between the two, when the lifting power module drives the gland 30 to press down, the gap between the inner cavity body 11 and the inner bottom plate 12 can be automatically sealed, realizing the sealing of the inner container 10. When the lifting power module drives the gland 30 to rise, the liquid channel 13 between the inner cavity body 11 and the inner bottom plate 12 can be automatically opened, realizing the automatic discharge of the liquid inside the inner container 10, and the control of the antigen repair device by the controller is more reasonable and simple.

[0125] Optionally, as Figure 11 , Figure 12 ,Figure 13 It is shown that the sliding connection mode between the inner bin body 11 and the inner bottom plate 12 is as follows: a first mounting seat 14 is arranged on the inner bottom plate 12, a second mounting seat 15 is arranged on the inner bin body 11, a vertically extending sliding hole is arranged on the first mounting seat 14, a round hole is arranged on the second mounting seat 15, and a fastener (such as a bolt) passes through the two holes of the two mounting seats. The fastener can slide up and down in the sliding hole, so as to realize that the inner bin body 11 can slide up and down relative to the inner bottom plate 12 within a certain stroke. The elastic member can be a spring or rubber with certain elasticity, etc. The elastic member can be arranged between the first mounting seat 14 and the second mounting seat 15. In other embodiments, the elastic member can also be directly arranged between the bottom of the inner bin body 11 and the inner bottom plate 12.

[0126] In other embodiments, the inner bin body 11 and the inner bottom plate 12 can also be integrally formed. The inner container 10 is a single integral structural member. In this way, there is no need to configure the second seal 62. When it is necessary to drain the liquid inside the inner container 10, the liquid extraction pipe can be put into the inner container 10 to extract the liquid inside the inner container 10, or a drain pipe with a valve is arranged on the inner container 10. When it is necessary to drain the liquid, the valve of the drain pipe of the inner container 10 is controlled to open.

[0127] In one embodiment, when a liquid passage 13 is formed between the inner bin body 11 and the inner bottom plate 12 when the gland 30 is in the first position, in order to improve the efficiency of draining the liquid inside the inner container 10, referring to Figure 9 、 Figure 10 、 Figure 14 ,a first diversion slope 103 is arranged on the inner wall of the inner bin body 11, and a second diversion slope 104 is arranged on one side of the inner bottom plate 12 close to the first opening 102. Both the first diversion slope 103 and the second diversion slope 104 are used to guide the liquid in the water tank 101 inside the inner container 10 to the outside and lower side of the inner container 10, so as to guide the liquid in the water tank 101 to the liquid passage 13 after the gland 30 rises to the first position. Among them, the first diversion slope 103 is inclined from the side close to the first opening 102 to the side close to the second seal 62 in the direction of the outside of the inner container 10. The second diversion slope 104 is inclined from the central area of the bottom plate to the edge area of the bottom plate in the direction away from the first opening 102. Taking the inner bin body 11 being arranged on the upper side of the inner bottom plate 12 as an example, the first diversion slope 103 is arranged in the lower area of the inner wall of the inner bin body 11. As shown in Figure 14 ,the first diversion slope 103 is inclined outward from top to bottom, and the second diversion slope 104 is inclined downward from inside to outside.

[0128] It can be understood that by providing the second diversion inclined surface 104 on the upper side of the inner bottom plate 12, not only the liquid discharge efficiency is improved, but also the liquid can be prevented from remaining inside the inner container 10. In addition, the provision of the first diversion inclined surface 103 and the second diversion inclined surface 104 is also conducive to cleaning the inside of the inner container 10. For example, after antigen repair is completed, a cleaning liquid is introduced into the inner container 10, and the cleaning liquid can flow along the first diversion inclined surface 103 and / or the second diversion inclined surface 104 to the liquid channel 13.

[0129] Optionally, first diversion inclined surfaces 103 are provided on both sides of the inner surface of the inner chamber body 11 along the x direction, and two second diversion inclined surfaces 104 are provided on the inner bottom plate 12 along the x direction. As Figure 14 shown, the top of the inner bottom plate 12 has a shape that is higher in the middle and lower on both sides. The first diversion inclined surface 103 and the second diversion inclined surface 104 are used to guide the liquid in the inner water tank 101 from the middle to both sides and downward, improving the efficiency of liquid discharge in the inner container 10 and preventing liquid from remaining. Taking the x direction as the left-right direction as an example, the first diversion inclined surface 103 on the left cooperates with the second diversion inclined surface 104 on the left, and the first diversion inclined surface 103 on the right cooperates with the second diversion inclined surface 104 on the right to guide the liquid in the inner water tank 101 to both the left and right sides, making it easier for the liquid in the inner water tank 101 to enter the outer water tank 201 through the liquid channels 13 on both sides.

[0130] In one embodiment, as Figure 9 、 Figure 10 、 Figure 13 、 Figure 14 shown, the inner bottom plate 12 includes a second boss 122 and a base plate 121 surrounding the outside of the second boss 122. The height of the base plate 121 is less than the height of the second boss 122. The second boss 122 is provided in the middle area of the base plate 121. The second boss 122 extends into the inside of the inner chamber body 11. The second seal 62 is provided between the lower side surface of the inner chamber body 11 and the upper side surface of the base plate 121. When the pressure cover 30 is in the second position and antigen repair heating is performed, the second seal 62 is clamped between the inner chamber body 11 and the base plate 121, and the second boss 122 is inserted into the inside of the inner container 10. The second boss 122 is used to limit the lateral relative movement between the inner chamber body 11 and the inner bottom plate 12, ensuring that the inner chamber body 11 can stably press on the base plate 121 of the inner bottom plate 12, ensuring the sealing effect on the lower side of the inner container 10 and ensuring the high-pressure heating effect.

[0131] In one embodiment, the antigen repair device includes an inner container 10 and an outer container 20, and the inner container 10 is disposed inside the outer container 20. The first opening 102 of the inner container 10 and the second opening 202 of the outer container 20 are both located on the upper side. When the pressure cover 30 is in the first position, the first opening 102 is located on the side of the second opening 202 away from the pressure cover 30, that is, the position of the first opening 102 is lower than the position of the second opening 202.

[0132] Among them, the liquid in the inner water tank 101 can enter the outer water tank 201 through the first opening 102, and when the inner water tank 101 is full of liquid, the liquid can be discharged into the outer water tank 201 through the first opening 102. Of course, the liquid in the outer water tank 201 can enter the inner water tank 101 through the first opening 102. When it is necessary to inject a reagent into the inner water tank 101, the reagent can be first injected into the outer water tank 201. The reagent can enter the inner water tank 101 either through the liquid channel 13 between the inner container body 11 and the inner bottom plate 12 or through the first opening 102 above the inner container 10. For these two ways of injecting the reagent into the inner water tank 101, there is no need to configure a dedicated pipeline for supplying liquid to the inner container 10, and only the outer container 20 needs to be supplied with liquid.

[0133] In one embodiment, the inner container 10 includes an inner container body 11 and an inner bottom plate 12. The first opening 102 is provided on the inner container body 11, and the inner bottom plate 12 is provided on the side of the inner container body 11 facing away from the first opening 102. The outer container 20 includes an outer container body 21 and an outer bottom plate 22. The second opening 202 is provided on the outer container body 21, and the outer bottom plate 22 is provided on the side of the outer container body 21 facing away from the second opening 202.

[0134] In one embodiment, the inner container 10 includes an inner container body 11 and an inner bottom plate 12. The first opening 102 and the inner bottom plate 12 are provided on opposite sides of the inner container body 11. The inner wall of the inner container body 11 and the surface of the inner bottom plate 12 enclose to form the inner water tank 101. The outer container 20 includes an outer container body 21 and an outer bottom plate 22. The second opening 202 and the outer bottom plate 22 are provided on opposite sides of the outer container body 21. The outer wall of the outer container body 21 and the surface of the outer bottom plate 22 enclose to form the outer water tank 201. Among them, the heating assembly 50 is provided between the inner bottom plate 12 and the outer bottom plate 22. In this way, on the one hand, the heating assembly 50 can be arranged at the bottom of the inner container 10. When the heating assembly 50 generates heat, the heat is conducted from the bottom of the inner container 10 to the inside of the inner container 10. On the second hand, a relatively sealed installation cavity for accommodating the heating assembly 50 can be formed by enclosing between the inner bottom plate 12 and the outer bottom plate 22. The liquid in the outer water tank 201 and the inner water tank 101 will not contact the heating assembly 50, providing waterproof protection for the heating assembly 50. On the third hand, the heating assembly 50 is not located inside the inner container 10, avoiding the heating assembly 50 from hindering the capping 30 from sealing the inner container 10, which is beneficial to the inner container 10 to be sealed for high-pressure heating.

[0135] Among them, the heating assembly 50 can heat the liquid inside the inner container 10 through resistance heating elements such as resistance wires, heating tubes, and heating plates, or can also heat the liquid inside the inner container 10 based on the electromagnetic heating principle and the microwave heating principle.

[0136] In one embodiment, the antigen retrieval device further includes a first temperature sensor 81 disposed in the inner water tank 101. The controller controls the power, on / off time of the heating component 50 according to the liquid temperature information in the inner water tank 101 detected by the first temperature sensor 81, so as to control the temperature inside the inner container 10 according to the experimental requirements.

[0137] In one embodiment, the antigen retrieval device further includes a second temperature sensor 82 disposed in the outer water tank 201. The controller is based on the liquid temperature information in the outer water tank 201 detected by the second temperature sensor 82.

[0138] Exemplarily, after the antigen retrieval is completed, the heating component 50 is turned off to stop heating. It is also necessary to cool down the antigen retrieval liquid inside the inner container 10. Cooling liquid for temperature reduction can be injected into the outer water tank 201 of the outer container 20. The liquid temperature in the outer water tank 201 is lower than that in the inner water tank 101. Through the way of heat conduction, the outer water tank 201 can cool the inner container 10, thereby reducing the temperature of the antigen retrieval liquid inside the inner container 10. Compared with the conventional cooling method of placing the antigen retrieval liquid in a room temperature environment and naturally cooling it to room temperature, the cooling method of using the liquid in the outer container 20 to cool the antigen retrieval liquid in the inner container 10 in this embodiment is more efficient. Among them, through the cooperation of the first temperature sensor 81 and the second temperature sensor 82, the temperature difference between the inner and outer water tanks 201 can be controlled. While increasing the cooling rate of the antigen retrieval liquid in the inner water tank 101, the temperature change of the antigen retrieval liquid can be monitored during the cooling process, avoiding the destruction of the configuration of the tissue section (antigen) on the glass slide 900 or the decline of the detection effect due to too low temperature of the antigen retrieval liquid in the inner water tank 101.

[0139] Among them, during the cooling, it is better that the tissue section on the glass slide 900 remains infiltrated by the antigen retrieval liquid. After the antigen retrieval liquid drops to room temperature or other target temperatures, subsequent washing steps and immunohistochemical staining steps can be carried out.

[0140] It can be understood that the appropriate cooling rate and end temperature of the antigen retrieval liquid in the inner container 10 can be set according to the specific requirements of the antigen retrieval liquid and experimental conditions. The inner container 10 is located inside the outer container 20. When the cooling liquid for temperature reduction is injected into the outer container 20, the cooling liquid for temperature reduction can evenly surround the outer wall of the inner container 10 and evenly cool the antigen retrieval liquid inside the inner container 10, with good effect.

[0141] In other embodiments, the antigen retrieval liquid and the tissue sample on the glass slide 900 can also be cooled by other means. In other embodiments, the second temperature sensor 82 disposed in the outer water tank 201 can also have other uses.

[0142] In one embodiment, the antigen repair device further includes a pressure relief component, which includes a pressure relief pipe 84 and a pressure relief valve 83. The pressure relief valve 83 can be installed on the device housing 90. One end of the pressure relief pipe 84 is connected to the gland 30, and the other end is connected to the pressure relief valve 83. Optionally, the gland 30 is provided with an air hole, one end of the pressure relief pipe 84 is connected to the air hole, and the outer wall of the pressure relief pipe 84 is closely attached to the hole wall of the air hole for sealing cooperation. The pressure relief valve 83 can be an electronic valve, and the controller can control the pressure relief valve 83 to adjust to the open state and the closed state. When the gland 30 is in the second position and the pressure relief valve 83 is in the open state, the inside of the inner container 10 is communicated with the outside of the inner container 10 through the pressure relief valve 83; when the gland 30 is in the second position and the pressure relief valve 83 is in the open state, the inside of the inner container 10 is communicated with the outside of the inner container 10 through the pressure relief valve 83. The setting of the pressure relief valve 83 is for two purposes. On the one hand, it is to prevent excessive pressure. As the temperature inside the inner container 10 rises, the pressure inside the inner container 10 gradually increases, and timely pressure relief can ensure safety. On the second hand, it can control the temperature and the heating time. Pressure relief can effectively reduce the temperature inside the inner container 10, avoiding damage to the antigen or other tissue sections on the slide 900 due to excessive temperature. Moreover, in the method of antigen repair using high-pressure heating, the length of the heating time has a direct impact on the repair effect, and the heating time can be controlled by pressure relief.

[0143] Among them, the pressure relief valve 83 is fixed to the housing 90, and the pressure relief valve 83 is connected to the controller through an electric wire to realize the automatic control of the pressure relief valve 83 by the controller. A flexible and deformable pressure relief pipe 84 is provided to connect the pressure relief valve 83 and the air hole on the gland 30, which can avoid pulling the electric wire connected to the pressure relief valve 83 during the process of the lifting power module driving the gland 30 to lift and lower, and prevent the electric wire from loosening or being damaged after being pulled, ensuring the reliable control of the opening and closing of the pressure relief valve 83 by the controller.

[0144] In other embodiments, the pressure relief pipe 84 may not be provided, and only the pressure relief valve 83 is provided. The pressure relief valve 83 is provided on the inner gland 30 or the inner container 10. The gland 30 is provided with an air hole, and the pressure relief valve 83 is installed in the air hole.

[0145] For the lifting power module, various driving methods can be adopted as long as it can control the movement of the gland 30 between the first position and the second position and control the gland 30 to stay in the second position according to requirements. The following provides a configuration method of the lifting power module, which can achieve uniform pressurization of the gland 30.

[0146] In the description of this article, it should be understood that the orientation or positional relationships such as "upper", "lower", "left", "right", etc. are based on the orientation or positional relationships shown in the drawings, and are only for the convenience of description and simplifying operations, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation to the present utility model. In addition, the terms "first" and "second" are only used for distinction in description and have no special meaning.

[0147] In the description of this specification, the description referring to terms such as "one embodiment", "example", etc. means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present utility model. In this specification, the schematic expressions of the above terms do not necessarily refer to the same embodiment or example.

[0148] In addition, it should be understood that although this specification is described according to embodiments, not every embodiment only contains an independent technical solution. This narrative way of the specification is only for clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

[0149] The technical principle of the present utility model has been described above in conjunction with specific embodiments. These descriptions are only for explaining the principle of the present utility model and cannot be construed as a limitation to the protection scope of the present utility model in any way. Based on the explanations herein, those skilled in the art can think of other specific implementation manners of the present utility model without creative efforts, and these manners will fall within the protection scope of the present utility model.

Claims

1. A cross-type lifting power module, characterized in that: Applied to antigen repair device; the cross-type lifting power module includes: A driving mechanism (41); The lifting mechanism comprises a pressing cover (30), wherein the pressing cover (30) is used to seal the opening of the container; A cross frame (42) comprises two brackets (421); the two ends of the bracket (421) are a first frame end (422) and a second frame end (423); the bracket (421) comprises a hinged portion (424) located between the first frame end (422) and the second frame end (423); the hinged portions (424) of the two brackets (421) are hinged; the second frame ends (423) of the two brackets (421) in the cross frame (42) are both in driving connection with the lifting mechanism; The output end of the driving mechanism (41) is drivingly connected to the first frame end (422) of the support (421), and the driving mechanism (41) is used to drive the two supports (421) in the cross frame (42) to open or close, so as to drive the pressure cover (30) to rise or fall.

2. The cross-type lifting power module according to claim 1, characterized in that: It comprises two cross frames (42), and the two cross frames (42) are arranged at intervals.

3. The cross-type lifting power module according to claim 2 is characterized in that: The lifting mechanism comprises a transmission seat (43), the pressure cover (30) is connected to the transmission seat (43), and the second frame end (423) of each of the brackets (421) is transmission-connected to the transmission seat (43).

4. The cross-type lifting power module according to claim 3 is characterized in that: The transmission seat (43) is provided with a slide groove (431), and the cross-type lifting power module includes a sliding rod (442); The first first frame end (422) of the first cross frame (42) is connected to the first first frame end (422) of the second cross frame (42) through at least one sliding rod (442); the second first frame end (422) of the second cross frame (42) is connected to the second first frame end (422) of the second cross frame (42) through at least one sliding rod (442); the sliding rod (442) is slidably installed in the sliding groove (431).

5. The cross-type lifting power module according to any one of claims 1 to 4, characterized in that: It also includes a screw rod (451) assembly; the screw rod (451) assembly includes a screw rod (451), a first shaft sleeve (452) and a second shaft sleeve (453); the screw rod (451) is provided with a first rod segment and a second rod segment with opposite spiral directions; the first shaft sleeve (452) is threadedly connected to the first rod segment, and the second shaft sleeve (453) is threadedly connected to the second rod segment; when the screw rod (451) rotates, the first shaft sleeve (452) and the second shaft sleeve (453) approach each other or move away from each other; The cross-type lifting power module also includes a shell (90); the first end of the screw rod (451) is transmission-connected to the driving mechanism (41), and the second end of the screw rod (451) is rotatably connected to the shell (90); the two first frame ends (422) in the cross frame (42) are respectively connected to the first shaft sleeve (452) and the second shaft sleeve (453).

6. The cross-type lifting power module according to claim 5, characterized in that: The gearbox (47) further comprises a reduction gearbox (47), wherein the output end of the driving mechanism (41) is drivingly connected to the input end of the reduction gearbox (47), and the output end of the reduction gearbox (47) is drivingly connected to the first end of the lead screw (451); the reduction gearbox (47) comprises a worm and a worm wheel, the driving mechanism (41) is drivingly connected to the worm, the worm wheel is drivingly connected to the worm, and the worm wheel is drivingly connected to the first end of the lead screw (451); And / or, the driving mechanism (41) is a stepping motor.

7. The cross-type lifting power module according to any one of claims 1 to 4, characterized in that: It also includes a shell (90) and a transverse guide rail (461) installed on the shell (90), and one or both of the first frame ends (422) of the two brackets (421) of the cross frame (42) are slidably connected to the transverse guide rail (461); when the cross frame (42) is opened or closed, the first frame end (422) slides along the transverse guide rail (461).

8. The cross-type lifting power module according to any one of claims 1 to 4, characterized in that: It also includes a shell (90) and a vertical guide rail (462) installed on the shell (90); at least one hinged portion (424) of the cross frame (42) is slidably connected to the vertical guide rail (462) via a connecting shaft (441); when the cross frame (42) is opened or closed, the connecting shaft (441) slides along the vertical guide rail (462).

9. The cross-type lifting power module according to claim 8, characterized in that: It also includes a connecting shaft (441), the hinged parts (424) of the two brackets (421) in the cross frame (42) are rotatably mounted on the connecting shaft (441), and the two ends of the connecting shaft (441) are respectively slidably connected to two vertical guide rails (462).

10. An antigen retrieval device, characterized in that: It comprises the cross-type lifting power module as claimed in any one of claims 1 to 9, and also comprises an inner container (10) and a pressure cover (30); An inner water tank (101) is provided inside the inner container (10), and a first opening (102) connected to the inner water tank (101) is provided on one side of the inner container (10); the lifting power module is used to drive the pressure cover (30) to move from a first position to a second position, and when the pressure cover (30) is in the first position, the pressure cover (30) is separated from the inner container (10); when the pressure cover (30) is in the second position, the pressure cover (30) covers the first opening (102).

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