Full-automatic microbial sample inoculation device

Abstract

In order to solve the problems that in the prior art, sample treatment is not uniform, and personnel are easily infected with germs, the utility model provides a full-automatic microbial sample inoculation device which comprises a rack mounting table top, a sample treatment device and a sample auxiliary device, a sample inoculation platform and an automatic sample inoculation mechanism are arranged on the two sides of the mounting table top of the rack respectively, and the automatic sample inoculation mechanism is provided with an inoculation piece of an elastic roller type structure; the sample weighing and diluting mechanism comprises a weighing sensor, a liquid adding rotary swing arm with a synchronous belt wheel structure and a peristaltic pump connected with a diluent bottle. The full-automatic sample processing device realizes full-automatic sample processing, and solves the problems that the existing sample processing is not uniform, and personnel are infected with germs.

Description

Technical Field

[0001] The utility model relates to the technical field of microorganism inoculation, in particular to a full-automatic microorganism sample inoculation device. Background Technique

[0002] In clinical medical treatment, inspection institutions, research institutes, etc., the inspection of microorganism samples is a prerequisite step for most genomics, chemistry and clinical experiments. Its quality directly affects the results of subsequent data analysis, and the inoculation of microorganism samples is an important link in the detection.

[0003] At present, there are two inoculation methods on the culture dish with culture medium: the first is to completely rely on manual scribing with an inoculation loop to operate; the second is to be realized by manual combination with a full-automatic or semi-automatic instrument.

[0004] Manual scribing requires the staff to be in close contact with the sample, resulting in the risk of biological infection for the staff themselves; secondly, the inoculation result may be incorrect due to the randomness, non-standardization and errors of personnel operation; moreover, the manual operation has a large workload, takes a long time, and the efficiency cannot be guaranteed.

[0005] In order to solve the above problems, a Chinese invention patent with the patent number CN109897768B proposes a microorganism automatic inoculation system, including an inoculation table. An inoculation needle positioning mechanism, a pre-inoculation disinfection device, a cooling box, a sampling bottle, a test tube fixing device and a post-inoculation disinfection device are successively arranged on the inoculation table from left to right. A support frame is also arranged on the inoculation table, and an inoculation needle grasping mechanism is arranged on the support frame. The inoculation needle grasping mechanism is slidably connected to the support frame, and a first driving mechanism is arranged between the inoculation needle grasping mechanism and the support frame. However, this invention does not have excellent technical features in the positioning accuracy of automatic inoculation, and it is easy to cause waste and increase costs. Content of the Utility Model

[0006] The utility model mainly solves the problems of non-uniformity of sample processing and easy infection of pathogens by personnel in the prior art, and provides a full-automatic microorganism sample inoculation device.

[0007] The above technical problems of the utility model are mainly solved by the following technical solutions:

[0008] A fully automatic microbial sample inoculation device, characterized in that it includes a frame mounting table, a sample processing device for completing sample operations, and a sample auxiliary device for providing corresponding auxiliary objects. On both sides of the frame mounting table, there are respectively a sample inoculation platform and a sample automatic inoculation mechanism. The sample automatic inoculation mechanism is provided with an inoculation part with an elastic roller structure. The sample processing device includes a sample weighing and dilution mechanism, and the sample weighing and dilution mechanism includes a weighing sensor, a liquid adding rotary swing arm with a synchronous pulley structure, and a peristaltic pump connected to a diluent bottle. The purpose of the present utility model is to achieve full-process automatic processing of samples.

[0009] As a preferred solution, the sample processing device includes a sample shaking and mixing mechanism provided with a connecting shaft rod. The connecting shaft rod is an eccentric structure, and an eccentric connecting plate is provided on the connecting shaft rod. When the motor operates, an oscillating effect is achieved.

[0010] As a preferred solution, the sample processing device further includes a sample pipetting mechanism provided with a two-axis linear module and a pipetting module, realizing the function of freely moving the pipetting module in a plane.

[0011] As a preferred solution, the inoculation part is curved, enhancing the elastic performance.

[0012] As a preferred solution, the inoculation part is provided with movable balls, realizing the elastic function of the inoculation part. During the inoculation process, the sample liquid can be better separated, and at the same time, the surface of the culture medium in the culture dish can be protected from being scratched.

[0013] As a preferred solution, the sample auxiliary device includes a sample bottle loading mechanism provided with a hollow rotating platform for sample bottle loading, a sample bottle transfer mechanism provided with a sample bottle transfer rotary grasping module, a Tip head loading mechanism provided with a Tip head loading linear module, an inoculation part loading mechanism provided with an inoculation part loading linear module, and a culture dish operation mechanism.

[0014] As a preferred solution, the sample bottle loading mechanism, the Tip head loading mechanism, and the inoculation part loading mechanism are all in a quick-release placement mode, facilitating replacement and disassembly.

[0015] As a preferred solution, the sample bottle transfer mechanism and the culture dish operation mechanism are both provided with jaws with external knurling processing.

[0016] Therefore, the advantages of the present utility model are:

[0017] Full-process automatic processing of samples is realized, solving the problems of non-uniformity in existing sample processing and personnel being infected with germs. Description of the Drawings

[0018] Figure 1 It is the structural diagram of the present utility model.

[0019] Figure 2 It is the structural diagram of the sample automatic inoculation mechanism of the present utility model.

[0020] Figure 3 It is the structural diagram of the Tip feeding mechanism of the present utility model.

[0021] Figure 4 It is the structural diagram of the sample vial feeding mechanism of the present utility model.

[0022] Figure 5 It is the structural diagram of the sample vial transfer mechanism of the present utility model.

[0023] Figure 6 It is the structural diagram of the sample weighing and dilution mechanism of the present utility model.

[0024] Figure 7 It is the structural diagram of the sample shaking and mixing mechanism of the present utility model.

[0025] Figure 8 It is the structural diagram of the sample pipetting mechanism of the present utility model.

[0026] Figure 9 It is the structural diagram of the sample inoculation platform of the present utility model.

[0027] Figure 10 It is the structural diagram of the culture dish labeling mechanism of the present utility model.

[0028] Figure 11 It is the structural diagram of the culture dish conveying mechanism of the present utility model.

[0029] In the figure: 1. Rack mounting tabletop; 2. Sample bottle loading mechanism; 3. Sample bottle transfer mechanism; 4. Tip head loading mechanism; 5. Sample bottle barcode scanning mechanism; 6. Sample weighing and dilution mechanism; 7. Sample shaking and mixing mechanism; 8. Sample pipetting mechanism; 9. Sample inoculation platform; 10. Inoculation part loading mechanism; 11. Sample automatic inoculation mechanism; 12. Petri dish opening and closing mechanism; 13. Petri dish conveying mechanism; 14. Petri dish barcode scanning mechanism; 15. Petri dish labeling mechanism; 16. Petri dish storage mechanism; 21. Sample bottle loading servo motor; 22. Sample bottle loading hollow rotary platform; 23. Sample bottle loading circumferential platform; 24. Sample bottle placement box; 31. Sample bottle transfer X-axis linear module; 32. Sample bottle transfer Y-axis linear module; 33. Sample bottle transfer Z-axis linear module; 34. Sample bottle transfer rotary grasping module; 35. Sample bottle transfer gripper; 41. Tip head; 42. Tip head device box; 43. Tip head loading linear module; 44. Tip head loading servo motor; 61. Peristaltic pump; 62. Weighing sensor; 63. Liquid adding rotary swing arm; 64. Sample weighing and dilution stepping motor; 71. Shaking bottle placement rack; 72. Connecting shaft rod; 73. Eccentric connecting plate; 74. DC brushless motor; 81. Pipetting module; 82. Sample pipetting rotary grasping module; 83. Sample pipetting X-axis linear module; 84. Sample pipetting Z-axis linear module; 91. Sample inoculation X-axis linear module; 92. Sample inoculation hollow rotary platform; 93. Sample inoculation electric claw; 111. Clamping device; 112. Elastic inoculation part; 131. Petri dish conveying X-axis linear module; 132. Petri dish conveying Z-axis linear module; 133. Petri dish conveying rotary grasping module; 134. Petri dish conveying gripper; 151. Petri dish conveying stepping motor; 152. Detection inductor; 153. Origin inductor; 154. Petri dish synchronous pulley; 155. Barcode label placement device; 156. Barcode label collection device; 157. Barcode label conveying motor; 241. Sample bottle. Detailed implementation manners

[0030] The technical solution of the present utility model will be further specifically described below through embodiments in conjunction with the accompanying drawings.

[0031] Embodiment 1:

[0032] A fully automatic microorganism sample inoculation device, comprising a frame mounting table 1, a sample processing device and a sample auxiliary device. On both sides of the frame mounting table 1, there are respectively provided a sample inoculation platform 9 and a sample automatic inoculation mechanism 11. The sample automatic inoculation mechanism 11 is provided with an inoculation member having an elastic roller structure. The sample processing device includes a sample weighing and diluting mechanism 6, and the sample weighing and diluting mechanism 6 includes a weighing sensor 62, a liquid adding rotary swing arm 63 with a synchronous pulley structure, and a peristaltic pump 61 connected to a diluent bottle. The utility model realizes the full-process automatic processing of samples, and solves the problems of non-uniformity in existing sample processing and personnel infection with germs, etc.

[0033] The utility model is internally provided with a quantity monitoring system, and relies on the quantity monitoring system for early warning feedback. The quantity monitoring system includes a sample bottle quantity monitoring module, a Tip head quantity monitoring module, an inoculation member quantity monitoring module, a culture dish quantity monitoring module and a bar code label monitoring module.

[0034] The sample processing device includes a sample weighing and diluting mechanism 6 that can automatically obtain and add the required dose of diluent, a sample shaking and mixing mechanism 7 for mixing the sample and the reagent solution, and a sample pipetting mechanism 8 for sucking the sample and adding it to a specified position in the culture dish.

[0035] The sample auxiliary device includes a sample bottle loading mechanism 2 that moves the sample bottle to a specified position and is provided with a sample bottle loading hollow rotary platform 22, a sample bottle transfer mechanism 3 that grabs and moves the sample bottle and is provided with a sample bottle transfer rotary grabbing module 34, a Tip head loading mechanism 4 that transports Tip heads 41 and is provided with a Tip head loading linear module, an inoculation member loading mechanism 10 that transports the inoculation member to the inoculation member taking position and is provided with an inoculation member loading linear module, and a culture dish operating mechanism for controlling the actions of the culture dish. The culture dish operating mechanism includes a culture dish opening and closing cover mechanism 12, a culture dish conveying mechanism 13, a culture dish bar code scanning mechanism 14, a culture dish labeling mechanism 15 and a culture dish storage mechanism 16.

[0036] The sample bottle loading mechanism 2 includes a sample bottle loading servo motor 21, a sample bottle loading hollow rotary platform 22, a sample bottle loading circumferential platform 23, and a sample bottle placement box 24. This mechanism is provided with 5 sample bottle device boxes 24, and each device box can place 6 sample bottles 241. The loading servo motor 21 cooperates with the sample bottle loading hollow rotary platform 22 to perform a circular cyclic motion, moving the sample bottle 241 to a specified position, and the sample bottle loading mechanism 2 grabs it to the next working station; a sample bottle device box quick release pin jack position is provided at the bottom of the sample bottle device box 24. After each box of sample bottles 241 is grabbed, it is moved to the manual box replacement place to realize the replacement of the sample bottle device box 24 without stopping the machine.

[0037] The Tip head feeding mechanism 4 includes a linear module 43, a Tip head feeding servo motor 44, and a Tip head device box 42. The Tip head feeding linear module 43 makes a linear reciprocating motion to transport the Tip head 41 to the position where the pipette tip picks up the Tip head 41. There is a quick-release pin jack position for the Tip head device box at the bottom of the Tip head device box 42. After a whole box of Tips is used up, it is moved to the manual box replacement location to achieve the replacement of the Tip head device box 42 without stopping the machine.

[0038] The sample bottle transfer mechanism 3 and the petri dish operating mechanism are both equipped with jaws with external knurling, including the sample bottle transfer jaw 35 and the petri dish transfer jaw 134.

[0039] The sample bottle transfer mechanism 3 includes an XYZ-axis linear module, a sample bottle transfer servo motor, and a sample bottle transfer rotary grasping module 34. This mechanism realizes grasping the sample bottle 241 to the positions of barcode scanning, weighing and dilution, shaking, and sample pipetting; the sample bottle transfer mechanism 3 grasps the sample bottle 241 and moves it, unscrews and opens the cap. The sample bottle transfer jaw 35 is knurled to increase the grasping friction, prevent the sample bottle 241 from falling, and reduce the error rate of opening the cap; the XYZ three-axis linear module includes a sample bottle transfer X-axis linear module 31, a sample bottle transfer Y-axis linear module 32, and a sample bottle transfer Z-axis linear module 33, realizing displacements in three directions of XYZ to meet the moving positions of each station.

[0040] The petri dish operating mechanism specifically includes a petri dish opening and closing mechanism 12, a petri dish transfer mechanism 13, a petri dish barcode scanning mechanism 14, a petri dish labeling mechanism 15, and a petri dish storage mechanism 16. The sample bottle barcode scanning mechanism 5 is arranged on one side of the sample bottle transfer mechanism. The sample bottle transfer mechanism 3 moves the sample bottle 241 with a barcode to the position of the barcode scanner and then performs a barcode scanning and recognition action.

[0041] The petri dish opening and closing mechanism 12 includes a Z-axis linear module and a vacuum suction cup module to complete the automatic opening and closing of the petri dish; the Z-axis linear module makes a vertical up and down movement. When the petri dish on the sample inoculation platform 9 reaches below the vacuum suction cup module, the Z-axis linear module descends to suck the upper cover of the petri dish and then rises. After the sample inoculation platform 9 moves to the inoculation position to complete the inoculation operation and returns below the vacuum suction cup module, the Z-axis linear module descends to cover the upper cover of the petri dish and then rises.

[0042] The petri dish transfer mechanism 13 includes an XZ-axis linear module, a petri dish transfer servo motor, and a petri dish transfer rotary grasping module 133, which is used to clamp the petri dish to the petri dish labeling mechanism 15, the petri dish scanning mechanism 14, and the sample inoculation platform 9; the rotary grasping module can rotate the petri dish 360 degrees for scanning; the petri dish transfer gripper 134 is processed with a knurled surface to prevent the petri dish from falling; the XZ-axis linear module of this mechanism includes a petri dish transfer X-axis linear module 131 and a petri dish transfer Z-axis linear module 132. The XZ-axis linear module can grasp the petri dish in the petri dish storage mechanism and transfer it to the petri dish labeling mechanism 15, the petri dish scanning mechanism 14, and the sample inoculation platform 9, and can also grasp and place the inoculated petri dish into the petri dish storage structure 16.

[0043] The petri dish scanning mechanism 14 is arranged on one side of the petri dish labeling structure 15. After the petri dish transfer mechanism 13 moves the petri dish to the position of the scanner, the scanning operation is carried out; the hole position of the scanner mounting part can be adjusted in angle.

[0044] The petri dish labeling mechanism 15 includes a petri dish transfer stepper motor 151, a detection sensor 152, a home sensor 153, a petri dish synchronous pulley 154, a bar code label placing device 155, a bar code label collecting device 156, and a bar code label transfer motor 157. After receiving the petri dish, it automatically completes the labeling action and feeds back a signal after the labeling is completed. After the above detection sensor 152 detects the placement of the petri dish, the motor starts to transfer the petri dish to the labeling position for operation. After the labeling is completed, it is transferred to the labeling completion detection sensor 152; the above home sensor 153 always keeps the device in a standby state.

[0045] The petri dish storage mechanism 16 includes a petri dish storage mechanism servo motor, a petri dish storage mechanism hollow rotating platform, a petri dish storage mechanism circumferential platform, partition plates, petri dish placement racks, and distance sensors. This mechanism is provided with 8 petri dish placement racks, and each petri dish placement rack can hold 15 petri dishes. The partition plates divide the petri dish placement racks into two parts. One side is for un-inoculated petri dishes, and the other side is for collecting the inoculated petri dishes. The petri dish storage mechanism servo motor cooperates with the petri dish storage mechanism hollow rotating platform to perform a circular motion, moving the petri dish to the designated position, and the petri dish transfer mechanism 13 grasps the petri dish to the designated station; the petri dish quantity detection sensor knows the quantity of petri dishes on the placement rack through distance detection; the bottom of the petri dish placement rack is provided with a quick-release pin jack for the petri dish placement rack, and it is possible to perform non-stop operation when taking out or putting in petri dishes.

[0046] The sample weighing and dilution mechanism 6 includes a peristaltic pump 61 for controlling the flow rate of the diluent, a weighing sensor 62 for sample weighing, a sample weighing and dilution stepper motor 64, and a liquid adding rotary swing arm 63 with a synchronous pulley structure. The sample bottle transfer mechanism 3 grabs and places the sample bottle 241 on the weighing sensor 62 device. After the weighing sensor 62 obtains the sample weight, it automatically obtains the required amount of diluent in the corresponding proportion. The sample weighing and dilution stepper motor 64 rotates to turn the swing arm above the sample bottle, and the peristaltic pump works to suck an equal amount of diluent and add it to the sample bottle.

[0047] The sample shaking and mixing mechanism 7 is arranged on one side of the sample weighing and dilution mechanism 6. The shaking bottle placement rack 71 can place 4 sample bottles 241 at a time. After the sample bottle transfer mechanism 3 grabs and places the sample bottle on the shaking bottle placement rack 71, the sample and the reagent solution are mixed. The connecting shaft rod 72 of the structure is an eccentric structure. Specifically, an eccentric connecting plate 73 is provided on the connecting shaft rod 72. When the DC brushless motor 74 at the bottom operates, the shaking effect is achieved.

[0048] The sample pipetting mechanism 8 includes a two-axis linear module for sample pipetting, a servo motor, a pipetting module 81, and a sample pipetting rotary grasping module 82. The two-axis linear module for sample pipetting includes an X-axis linear module 83 for sample pipetting and a Z-axis linear module 84 for sample pipetting, which are used to clamp and unscrew the sample bottle cap and suck the sample and add it to a specified position in the culture dish. The X-axis linear module 83 for sample pipetting and the Z-axis linear module 84 for sample pipetting move the pipetting module 81 above the Tip head device box 42 to automatically press and pick up the Tip head 41. The X-axis linear module 83 for sample pipetting and the Z-axis linear module 84 for sample pipetting move the sample pipetting rotary grasping module 82 above the sample bottle 241, unscrew and open the sample bottle cap, and the pipetting module 81 sucks the sample liquid and drops it to the specified position in the culture dish.

[0049] The sample automatic inoculation mechanism 11 is provided with an XYZ three-axis linear module, a servo motor, and a clamping device 111, and automatically takes the inoculation part to the sample inoculation platform 9 for preset custom trajectory inoculation. The inoculation part at the top of the clamping device 111 is an elastic inoculation part 112 with an elastic roller structure. The elastic inoculation part 112 is curved, and the material is a resin flexible material with certain elastic properties. Secondly, the ball on the elastic inoculation part 112 is movable, realizing the elastic function of the inoculation part. During the inoculation process, the sample liquid can be better separated, and at the same time, the surface of the culture medium in the culture dish can be protected from being scratched. The sample automatic inoculation mechanism 11 is provided with a clamping device to prevent the culture dish from moving during the inoculation process, resulting in deviation of the inoculation trajectory.

[0050] The sample inoculation platform 9 includes a sample inoculation X-axis linear module 91, a sample inoculation hollow rotating platform 92, and a sample inoculation electric claw 93, which can move the culture dish loaded with the culture medium to the inoculation position, and can also realize the 360-degree rotation of the culture dish. During the inoculation process, the electric claw clamps the culture dish to ensure the accuracy of the inoculation trajectory. The XYZ three-axis linear module and the hollow rotating platform can realize scribing inoculation at all positions inside the culture dish.

[0051] The inoculation part feeding mechanism 10 includes an inoculation part feeding linear module, a servo motor, and an inoculation part device box. The inoculation part feeding linear module makes a linear reciprocating motion to transport the inoculation part to the position for picking up the inoculation part. The bottom of the inoculation part device box is provided with a quick-release pin jack position. After a whole box of inoculation parts is used up, it is moved to the manual box replacement place to realize the replacement of the inoculation part device box without stopping the machine.

[0052] The sample bottle feeding mechanism 2, the Tip head feeding mechanism 4, and the inoculation part feeding mechanism 10 all adopt a quick-release placement method to ensure the high efficiency and flexibility of the operation. This quick-release placement method enables the replacement of these mechanisms without stopping the machine, thus greatly improving the work efficiency. Specifically, the quick-release placement method adopts a pin hole positioning and picking method, that is, through precisely designed pin holes and positioning devices, to ensure the accurate positioning and stable installation of the mechanism. The advantage of this method is that it saves the time required for replacing the mechanism and avoids production interruption caused by machine stoppage. The operator only needs to simply disassemble the original mechanism and then accurately insert the new mechanism into the corresponding pin holes to complete the replacement. This fast and convenient replacement method provides convenience for the continuous operation of the production line and improves the production efficiency.

[0053] Embodiment 2:

[0054] The sample bottle placement box 24 (the sample bottle loaded with the sample), the Tip head device box 42 (loaded with Tip heads), the inoculation part device box (loaded with inoculation parts), and the culture dish (containing the culture medium) are pre-placed at the designated positions of the system; one end structure will automatically grab the sample bottle 241 to complete the operations of scanning code, weighing, and dilution, and then place it at the position for taking the sample liquid; one end structure will automatically pick up the Tip head 41 and move it to the position for taking the sample liquid to suck the sample liquid, and after sucking the sample liquid, move it to the sample inoculation platform 9; one end structure will grab the culture dish to complete the operations of labeling and scanning code, open the lid of the culture dish and place it on the sample inoculation platform 9; one end structure will automatically grab the inoculation part to the inoculation position. After the three-end structure completes the operations, the inoculation operation starts. After the inoculation is completed, the culture dish is retracted to the storage place.

[0055] The specific embodiments described herein are merely illustrative of the spirit of the present utility model. Those skilled in the art to which the present utility model pertains may make various modifications or supplements to the described specific embodiments or use similar means for substitution, but they will not deviate from the spirit of the present utility model or exceed the scope defined by the appended claims.

Claims

1. A fully automatic microbial sample inoculation device, characterized in that: It includes a rack mounting table, a sample processing device and a sample auxiliary device. A sample inoculation platform and a sample automatic inoculation mechanism are respectively arranged on both sides of the rack mounting table. The sample automatic inoculation mechanism is provided with an inoculation piece with an elastic roller structure. The sample processing device includes a sample weighing and dilution mechanism. The sample weighing and dilution mechanism includes a weighing sensor, a liquid adding rotating swing arm with a synchronous pulley structure and a peristaltic pump connected to a diluent bottle.

2. A fully automatic microbial sample inoculation device according to claim 1, characterized in that: The sample processing device comprises a sample oscillating and shaking mechanism provided with a connecting shaft, wherein the connecting shaft is an eccentric structure and an eccentric connecting plate is provided on the connecting shaft.

3. A fully automatic microbial sample inoculation device according to claim 2, characterized in that: The sample processing device also includes a sample pipetting mechanism provided with a sample pipetting two-axis linear module and a pipetting module.

4. A fully automatic microbial sample inoculation device according to claim 1, characterized in that: The inoculation piece is in a curved shape.

5. A fully automatic microbial sample inoculation device according to claim 1 or 4, characterized in that: The inoculating piece is provided with a movable ball.

6. A fully automatic microbial sample inoculation device according to claim 1, characterized in that: The sample auxiliary device includes a sample bottle loading mechanism provided with a sample bottle loading hollow rotating platform, a sample bottle transferring mechanism provided with a sample bottle transferring rotating grasping module, a Tip head loading mechanism provided with a Tip head loading linear module, an inoculation piece loading mechanism provided with an inoculation piece loading linear module, and a culture dish operating mechanism.

7. A fully automatic microbial sample inoculation device according to claim 6, characterized in that: The sample bottle loading mechanism, the tip head loading mechanism and the inoculation piece loading mechanism are all quick-release placement methods.

8. The fully automatic microbial sample inoculation device according to claim 6, characterized in that: The sample bottle transfer mechanism and the culture dish operation mechanism are both provided with clamping claws with knurling on the outside.

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