Capillary electrophoresis apparatus capable of efficiently analyzing size of nucleic acid fragment

Abstract

The utility model belongs to the technical field of capillary electrophoresis, and discloses a capillary electrophoresis apparatus capable of efficiently analyzing the size of a nucleic acid fragment, which comprises an electrophoresis apparatus body, a partition plate attached to the inside of the electrophoresis apparatus body, a sliding rod fixedly connected to the top end of the partition plate, a sliding block sleeved on the outer side of the sliding rod, and a round hole groove arranged on the rear side of the sliding block, a screwing block is slidably connected into the circular hole groove of the sliding block, a sliding rod is slidably connected to the center of the interior of the sliding block, the right side of the screwing block abuts against the outer side of the sliding rod, a supporting frame is fixedly connected to the front end of the sliding block, a rotating ring is sleeved with the supporting frame, and a detector is fixedly connected to the top end of the rotating ring; by means of the scheme, the capillary electrophoresis apparatus solves the problems that an existing capillary electrophoresis apparatus can only meet basic detection requirements and cannot gradually meet various specific requirements of the current biotechnology, especially the requirements for miniaturization, integration and convenience.

Description

Technical Field

[0001] The utility model belongs to the technical field of capillary electrophoresis, and specifically relates to a capillary electrophoresis instrument capable of efficiently analyzing the size of nucleic acid fragments. Background Technique

[0002] The capillary electrophoresis instrument mainly includes a high-voltage power supply, a capillary, a sample injection system, a detection system, and a data acquisition system. Among them, the high-voltage power supply usually adopts a stable and continuously adjustable DC power supply to provide a separation potential difference for the capillary electrophoresis instrument; the capillary is required to have chemical and electrical inertness, ultraviolet-visible light transmittance, flexibility, and high strength characteristics, and ordinary glass capillaries, quartz glass capillaries, and polytetrafluoroethylene capillaries can be used as the electrophoresis separation channel; the sample injection system is used to inject the sample or electrophoresis reaction solution into the capillary, and usually adopts methods such as electrokinetic injection, pressure difference injection, or diffusion injection.

[0003] At the same time, the patent with the application number 201821232271.X discloses a capillary electrophoresis instrument, including a mounting bracket, a capillary assembly is arranged on the mounting bracket, a moving platform assembly is arranged at a position corresponding to the cathode end of the capillary assembly on the mounting bracket, a glue filling assembly is arranged at a position corresponding to the anode end, and an optical detection assembly is arranged at a position corresponding to the detection window. The moving platform assembly is connected to the cathode end of the capillary assembly, the glue filling assembly is connected to the anode end of the capillary assembly, the optical detection assembly is connected to the detection window of the capillary assembly, and the capillary assembly, the moving platform assembly, the glue filling assembly, and the optical detection assembly are respectively connected to an embedded control assembly.

[0004] However, in the implementation of related technologies, it is found that the above-mentioned existing capillary electrophoresis instruments can only meet basic detection requirements and have gradually been unable to meet the specific needs of current biotechnology, especially the requirements of miniaturization, integration, and convenience. Content of the Utility Model

[0005] To solve the problems raised in the above background technique, the utility model provides a capillary electrophoresis instrument capable of efficiently analyzing the size of nucleic acid fragments, which has the advantages of improving detection, integrating the pressure power supply, capillary, sample injection system, detection system, and data acquisition system together to achieve integration, reducing the floor space, improving the detection efficiency, and meeting the rapid development requirements of gene diagnosis, transgenic food detection, personalized medicine, etc.

[0006] To achieve the above object, the present utility model provides the following technical solutions: A capillary electrophoresis instrument capable of efficiently analyzing the size of nucleic acid fragments, including an electrophoresis instrument body. Inside the electrophoresis instrument body, there is a fitting partition. At the top of the partition, there is a fixed slide bar. A slider is sleeved outside the slide bar. A round hole groove is opened at the rear of the slider. A screwing block is slidably connected inside the round hole groove of the slider. A slide bar is slidably connected at the central position inside the slider. The right side of the screwing block is tightly abutted against the outside of the slide bar. At the front end of the slider, there is a fixed support frame. A rotating ring is sleeved inside the support frame. At the top of the rotating ring, there is a fixed detector. At the bottom of the rotating ring, there is a fixed magnifying glass.

[0007] Preferably, at the bottom of the magnifying glass, there is a vessel. The bottom of the vessel is fitted against the top of the partition. A square groove is opened inside the vessel.

[0008] Preferably, a filter screen is slidably connected inside the square groove of the vessel. A number of groups of round hole grooves are opened on the outside of the filter screen.

[0009] Preferably, a tightening ring is fitted against the bottom of the filter screen. A screw rod is spirally connected inside the tightening ring. The screw rod penetrates through the inside of the filter screen.

[0010] Preferably, an electrode plate is fitted against the bottom of the cavity of the vessel. An electrode block is fixedly connected to the outside of the electrode plate.

[0011] Preferably, a round hole groove is opened at the top of the vessel. A buckle is slidably connected inside the round hole groove of the vessel. A rotating support plate is sleeved outside the buckle. A round hole groove is opened inside the rotating support plate. A test tube is slidably connected inside the round hole groove of the rotating support plate. There are a number of groups of test tubes.

[0012] Preferably, a top cover is rotatably connected to the top of the electrophoresis instrument body. An operation panel is fixedly connected to the front end of the top cover.

[0013] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0014] 1. By adding a microscope device inside the capillary body, the present utility model can extremely finely separate and detect nucleic acid fragments, and can accurately analyze the size of each fragment in a complex nucleic acid sample. Its fast and accurate characteristics make the research work more efficient and convenient. Its fast analysis speed enables researchers to obtain a large amount of accurate information in a short time, greatly improving the work efficiency.

[0015] 2. The utility model adds a filter device inside the vessel. When a nucleic acid sample enters one end of the capillary, under the action of the electric field generated by the high-voltage power supply, charged particles migrate electrophoretically in the capillary. Since the migration speeds of different charged particles are different, after a period of electrophoresis, different charged particles can be separated. During this process, it has the advantages of high separation efficiency, high sensitivity, and low cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0017] Figure 2 It is a schematic diagram of the structure of the detector of the utility model;

[0018] Figure 3 It is a schematic diagram of the structure of the electrophoresis apparatus body of the utility model;

[0019] Figure 4 It is a schematic diagram of the structure of the partition of the utility model;

[0020] Figure 5 It is a schematic diagram of the structure of the vessel of the utility model.

[0021] In the figure: 1. Electrophoresis apparatus body; 2. Upper cover; 3. Partition; 4. Slide block; 5. Screw block; 6. Slide bar; 7. Rotating ring; 8. Detector; 9. Magnifying glass; 10. Vessel; 11. Buckle; 12. Rotating support plate; 13. Test tube; 14. Filter; 15. Tightening ring; 16. Lead screw; 17. Electrode plate; 18. Electrode block; 19. Support frame; 20. Operation panel. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0022] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present utility model.

[0023] Such as Figures 1 to 5As shown in the figure, the utility model provides a capillary electrophoresis instrument capable of efficiently analyzing the size of nucleic acid fragments, which includes an electrophoresis instrument body 1. A partition 3 is attached to the inside of the electrophoresis instrument body 1. A slide bar 6 is fixedly connected to the top end of the partition 3. A slider 4 is sleeved on the outside of the slide bar 6. A round hole groove is opened at the rear side of the slider 4. A screwing block 5 is slidably connected inside the round hole groove of the slider 4. The center position of the slider 4 is slidably connected with the slide bar 6. The right side of the screwing block 5 is tightly abutted against the outside of the slide bar 6. The front end of the slider 4 is fixedly connected with a support frame 19. A rotating ring 7 is sleeved inside the support frame 19. A detector 8 is fixedly connected to the top end of the rotating ring 7. A magnifying glass 9 is fixedly connected to the bottom end of the rotating ring 7. Adjust the height of the magnifying glass 9 according to the height of the vessel 10. Rotate the screwing block 5 counterclockwise, push the slider 4 upward, and then screw the screwing block 5 clockwise to achieve fixation.

[0024] Specifically, adjust the focal length between the magnifying glass 9 and the material, and take pictures and records of the material. The bottom end of the vessel 10 is attached to the top end of the partition 3.

[0025] Furthermore, a filter screen 14 is slidably connected inside the square groove of the vessel 10.

[0026] Still further, a tightening ring 15 is attached to the bottom end of the filter screen 14. A lead screw 16 is helically connected inside the tightening ring 15. The lead screw 16 penetrates through the inside of the filter screen 14. By rotating the lead screw 16, the tightening ring 15 at the bottom end is lifted, and the tightening ring 15 raises the filter screen 14.

[0027] It should be noted that an electrode plate 17 is attached to the bottom end of the cavity of the vessel 10. An electrode block 18 is fixedly connected to the outside of the electrode plate 17. After the nucleic acid sample enters one end of the capillary, under the action of the electric field generated by the electrode plate 17, charged particles migrate electrophoretically in the capillary. Since the migration speeds of different charged particles are different, after a period of electrophoresis, different charged particles can be separated. During this separation process, the material is separated.

[0028] It should be noted that a round hole groove is opened at the top end of the vessel 10. A buckle 11 is slidably connected inside the round hole groove of the vessel 10. A rotating support plate 12 is sleeved on the outside of the buckle 11. A round hole groove is opened inside the rotating support plate 12. Put the solution into the inside of the test tube 13, and insert the rotating support plate 12 into the inside of the vessel 10, and several groups of test tubes 13 are provided.

[0029] It is worth introducing that the top end of the electrophoresis instrument body 1 is rotatably connected with an upper cover 2. Just close it when not in use. The operation panel 20 at the front end of the upper cover 2 is used to operate the electrophoresis instrument body 1.

[0030] Among them, the electrophoresis apparatus main body 1 is a prior art and will not be elaborated; at the same time, the present utility model also includes a power supply, a controller, a switch, etc., which are not the main technical points of this patent and will not be elaborated; the "front, back, left, and right" perspectives of this device are based on Figure 1 the direction of the illustrated figure.

[0031] Working principle:

[0032] First, the staff places the object to be detected inside the vessel 10, and adjusts the height of the magnifying glass 9 according to the height of the vessel 10. Rotate the screw block 5 counterclockwise, push the slider 4 upward, and then rotate the screw block 5 clockwise to fix it, and adjust the focal length between the magnifying glass 9 and the material, take pictures and records of the material. Finally, put the solution into the inside of the test tube 13, insert the rotating support plate 12 into the inside of the vessel 10, add a filter screen 14 device inside the vessel 10. The nucleic acid sample enters one end of the capillary, and then under the action of the electric field generated by the electrode plate 17, the charged particles migrate electrophoretically in the capillary. Since the migration speeds of different charged particles are different, after a period of electrophoresis, different charged particles can be separated. During this separation process, the material is separated. Finally, rotate the lead screw 16 to lift the tightening ring 15 at the bottom, and the tightening ring 15 raises the filter screen 14.

[0033] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not expressly listed, or also includes elements inherent to such process, method, article or device.

[0034] Although the embodiments of the present utility model have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principle and spirit of the present utility model. The scope of the present utility model is defined by the appended claims and their equivalents.

Claims

1. A capillary electrophoresis instrument capable of efficiently analyzing the size of nucleic acid fragments, comprising an electrophoresis instrument body (1), characterized in that: A partition (3) is fitted inside the electrophoresis instrument body (1), a sliding rod (6) is fixedly connected to the top of the partition (3), a slider (4) is sleeved on the outside of the sliding rod (6), a circular hole groove is opened on the rear side of the slider (4), a screw block (5) is slidably connected inside the circular hole groove of the slider (4), the sliding rod (6) is slidably connected at the inner center position of the slider (4), the right side of the screw block (5) is tightly arranged on the outside of the sliding rod (6), the front end of the slider (4) is fixedly connected to a support frame (19), a rotating ring (7) is sleeved inside the support frame (19), the top of the rotating ring (7) is fixedly connected to a detector (8), and the bottom of the rotating ring (7) is fixedly connected to a magnifying glass (9).

2. A capillary electrophoresis instrument capable of efficiently analyzing the size of nucleic acid fragments according to claim 1, characterized in that: A container (10) is provided at the bottom end of the magnifying glass (9), the bottom end of the container (10) is attached to the top end of the partition (3), and a square groove is provided inside the container (10).

3. A capillary electrophoresis instrument capable of efficiently analyzing the size of nucleic acid fragments according to claim 2, characterized in that: A filter screen (14) is slidably connected inside the square groove of the vessel (10), and a plurality of groups of circular hole grooves are opened on the outer side of the filter screen (14).

4. A capillary electrophoresis instrument capable of efficiently analyzing the size of nucleic acid fragments according to claim 3, characterized in that: The bottom end of the filter screen (14) is fitted with a tightening ring (15), the interior of the tightening ring (15) is spirally connected with a screw rod (16), and the screw rod (16) passes through the interior of the filter screen (14).

5. A capillary electrophoresis instrument capable of efficiently analyzing the size of nucleic acid fragments according to claim 2, characterized in that: An electrode plate (17) is attached to the bottom end of the cavity of the vessel (10), and an electrode block (18) is fixedly connected to the outer side of the electrode plate (17).

6. A capillary electrophoresis instrument capable of efficiently analyzing the size of nucleic acid fragments according to claim 5, characterized in that: A circular hole groove is formed at the top of the vessel (10), a buckle (11) is slidably connected inside the circular hole groove of the vessel (10), a rotating support plate (12) is sleeved on the outer side of the buckle (11), a circular hole groove is formed inside the rotating support plate (12), a test tube (13) is slidably connected inside the circular hole groove of the rotating support plate (12), and a plurality of groups of test tubes (13) are provided.

7. A capillary electrophoresis instrument capable of efficiently analyzing the size of nucleic acid fragments according to claim 1, characterized in that: The top end of the electrophoresis apparatus body (1) is rotatably connected to an upper cover (2), and the front end of the upper cover (2) is fixedly connected to an operation panel (20).

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