Precise perfusion dosing device for patch clamp experiment

Abstract

The utility model relates to the field of biological medicine experimental equipment, and discloses a precise perfusion dosing device for patch clamp experiments, which comprises an equipment seat, a fixing column is fixedly connected to the middle of the top end of the equipment seat, and a fixing plate is fixedly connected to the rear side of the top of the outer wall of the fixing column. Electric telescopic rods are fixedly connected to the left side and the right side of the rear portion of the top end of the fixing plate, a concave clamping plate is fixedly connected to one ends of the two electric telescopic rods, and a limiting clamping plate is fixedly connected to the front side of the top of the outer wall of the fixing column. According to the utility model, by opening the piston I, the liquid medicine flows into the gathering box due to gravity and is added to a tissue sample, the concave clamping plate and the plug rod of the electric telescopic rod are started to rise, the syringe generates suction force, the medicine in various medicine boxes enters the four-way pipe through the feeding pipe and the one-way valve I and reaches the syringe, and the piston I is closed, and the electric telescopic rod shrinks and recovers to the initial position; and medicines can be automatically supplemented and added.

Description

Technical Field

[0001] The utility model relates to the field of biomedical experimental equipment, in particular to a precision perfusion drug delivery device for patch clamp experiments. Background Art

[0002] A precision perfusion drug delivery device for patch clamp experiments is a biological experimental device for nerve cells or tissue samples. It precisely delivers drugs or other solutions into the experimental samples through microchannels and injection pump devices. This device is used in the fields of studying nerve transmission and drug action mechanisms. During the experiment, researchers can adjust the flow rate and drug concentration as needed to simulate different physiological conditions or pathological states. The precision and adjustability of this device have enabled it to be widely used in neurobiology and pharmacology research, providing reliable technical support and data basis for scientific research.

[0003] After retrieval, the Chinese patent publication number is: CN217651244U, which discloses a patch clamp perfusion drug delivery device, including a bottom plate. A bracket is fixedly installed on the top of the bottom plate. The number of brackets is two, and they are symmetrically distributed front and back. A box body is movably sleeved outside the two brackets. A worm is rotatably connected to the rear side of the inner cavity of the box body. A rotating rod is rotatably connected to the middle of the bottom of the inner cavity of the box body. A convex block is fixedly sleeved outside the rotating rod. A worm gear is fixedly installed on the top of the rotating rod. The worm gear meshes with the worm. Limit rods are fixedly installed on the front side and the rear side of the bottom of the inner cavity of the box body. A clamping rod is movably sleeved outside the limit rod. This utility model can quickly clamp the drainage tube, is convenient and fast to operate, improves the use efficiency of the device, can also adjust the use height of the device, effectively avoids the situation of bending and knotting under the drainage tube, has a simple structure and is convenient to operate, improving the practicability of the device. However, in the actual use process, when perfusing drugs or other solutions into the drainage tube, it needs to be removed or manually perfused, resulting in inconvenient operation, low drug delivery accuracy, and inability to meet the requirements of precisely controlling the liquid medicine flow rate and drug delivery time. Summary of the Utility Model

[0004] To make up for the above deficiencies, the utility model provides a precision perfusion drug delivery device for patch clamp experiments, aiming to improve the problems in the prior art that it needs to be removed or manually perfused, resulting in inconvenient operation, low drug delivery accuracy, and inability to meet the requirements of precisely controlling the liquid medicine flow rate and drug delivery time.

[0005] To achieve the above object, the utility model adopts the following technical solutions: A precision perfusion and drug administration device for patch clamp experiments, including an equipment base. In the middle of the top of the equipment base, a fixed column is fixedly connected. At the rear side of the top of the outer wall of the fixed column, a fixed plate is fixedly connected. On the left and right sides of the rear part of the top of the fixed plate, electric telescopic rods are fixedly connected. At one end of the two electric telescopic rods, a concave clamping plate is fixedly connected. At the front side of the top of the outer wall of the fixed column, a limit clamping plate is fixedly connected. A plurality of syringe barrels are arranged inside the limit clamping plate. A piston rod is arranged inside the syringe barrel. The top of the outer walls of the plurality of piston rods is engaged with the concave clamping plate. The bottom of the syringe barrel is fixedly connected with a first piston. The bottom of the first piston is fixedly connected with a four-way pipe. A first one-way valve is communicated with the rear side of the four-way pipe. At the rear side of the top of the equipment base, a multi-category medicine box is fixedly connected. The rear side of the first one-way valve is communicated with a feed pipe. The bottoms of the plurality of feed pipes are communicated with the multi-category medicine box. A second one-way valve is communicated with the bottom of the four-way pipe. The bottoms of the plurality of second one-way valves are communicated with a converging box. A liquid adding component is arranged at the bottom of the converging box. A cleaning mechanism is arranged inside the converging box. The cleaning mechanism is used to clean the pipeline to prevent bacterial infection.

[0006] Through the above technical solutions: When it is necessary to add medicine to the tissue sample, only need to open the first piston above the required medicine. At this time, due to the gravity of the liquid medicine itself, the liquid medicine will enter the converging box through the first piston and the second one-way valve, and the liquid medicine is added to the tissue sample through the liquid adding component. When the medicine in the syringe barrel is used up or needs to be replenished, at this time, start the electric telescopic rod to drive the concave clamping plate to move towards the top of the equipment base, and then drive the piston rod to move towards the top. At this time, suction will be generated in the syringe barrel, so that the corresponding medicine in the multi-category medicine box is sucked into the feed pipe, enters the four-way pipe through the first one-way valve, and finally enters the syringe barrel, thus completing the automatic addition of the liquid medicine. Then close the first piston. At this time, the electric telescopic rod can be started to contract and return to the initial position.

[0007] As a further description of the above technical solutions:

[0008] The cleaning mechanism includes two hollow grooves. The two hollow grooves are opened on the left and right sides inside the converging box. On the left side of the inner wall of the right hollow groove, a heating ring is fixedly connected. On the right side of the inner wall of the right hollow groove, a blower is fixedly connected. A plurality of air inlet holes are opened on the right rear side of the converging box. A filter screen is fixedly connected to the rear side of the converging box. A filter sponge is arranged on the inner wall of the left hollow groove. An exhaust baffle is arranged on the left side of the top of the converging box. The outer wall of the exhaust baffle penetrates through the converging box. A cleaning water pipe is arranged on the top of the converging box. A plurality of liquid distribution pipes are communicated with the outer wall of the cleaning water pipe. The hollow grooves are respectively communicated with the front sides of the corresponding four-way pipes. A water pipe valve is fixedly connected to the right side of the outer wall of the cleaning water pipe.

[0009] Through the above technical scheme: when the liquid addition is completed, the distilled water from the outside is guided through the cleaning water pipe, and the water pipe valve on the top is opened to allow the distilled water to enter the four-way pipe through the liquid separation pipe, thereby flushing the four-way pipe, the second one-way valve, the gathering box and the liquid addition component to prevent the residual medicine from affecting the next use. Then, the heating ring and the fan are started to blow dry hot air into the interior of the gathering box, thereby drying the inside, and the outside air will be filtered by the filter net, and the moisture inside the gathering box will be driven to the top of the filter sponge to absorb the moisture, and the air will pass through the filter sponge and be discharged through the exhaust baffle, thereby completing the cleaning and drying of the internal pipeline.

[0010] As a further description of the above technical solution:

[0011] The liquid adding assembly comprises a conical liquid draining block, which is connected to the bottom of the convergence box, a second piston is fixedly connected to the bottom of the conical liquid draining block, and a liquid adding pipe is connected to the bottom of the second piston.

[0012] Through the above technical solution: liquid is gathered through the conical liquid discharge block, the liquid can pass through by opening the second piston, and liquid is added through the liquid adding pipe.

[0013] As a further description of the above technical solution:

[0014] A lamp post is fixedly connected to the left side of the top of the equipment seat, a lighting lamp is fixedly connected to the right side of the lamp post, an alarm is fixedly connected to the left rear side of the top of the equipment seat, and a notch is opened at the top of the front end of the exhaust baffle.

[0015] Through the above technical solution: the visibility of the experimental operation can be improved through the lighting, which makes it convenient for the experimenters to connect the pipeline and adjust the valve operation; the alarm can be sounded by the alarm in the event of a fault; and the exhaust baffle can be easily pulled out through the recess.

[0016] As a further description of the above technical solution:

[0017] The front side of the outer wall of the convergence box is fixedly connected with a plurality of indicator lights, the rear side of the inner wall of the convergence box is fixedly connected with a plurality of laser emitters, and the front side of the inner wall of the convergence box is fixedly connected with a plurality of photosensitive sensors.

[0018] Through the above technical solution: light is emitted by a laser transmitter. When liquid flows through, the light will be refracted and cannot be received by the photosensor. At this time, the indicator light will light up, indicating that liquid is flowing through the pipe.

[0019] As a further description of the above technical solution:

[0020] On the front side of the outer wall of the multi-category medicine box, an observation window is provided, and a sealing frame is fixedly connected to the outside of the observation window.

[0021] Through the above technical solution: Through the observation window, the remaining situation of each medicine in the multi-category medicine box can be known.

[0022] As a further description of the above technical solution:

[0023] On the outer wall of the syringe barrel, a plurality of scale lines are provided, and an anti-slip net block is fixedly connected to the top of the piston rod.

[0024] Through the above technical solution: Through the scale lines, the volume drawn into the syringe barrel can be known, and through the anti-slip net block, the anti-slip ability of the piston rod can be improved.

[0025] As a further description of the above technical solution:

[0026] On the front side top of the limit clamping plate, a plurality of limit baffle plates are rotatably connected, and a plurality of clamping blocks are fixedly connected to the middle part of the front side of the limit clamping plate, and the limit baffle plates are engaged with the clamping blocks.

[0027] Through the above technical solution: By the engagement of the limit baffle plates and the clamping blocks, the syringe barrel can be prevented from falling.

[0028] The utility model has the following beneficial effects:

[0029] 1. In the utility model, by opening the piston 1, the liquid medicine flows into the converging box due to gravity and is added to the tissue sample. When the medicine is used up or needs to be replenished, the electric telescopic rod is started, the concave clamping plate and the piston rod rise, the syringe barrel generates suction, and the medicine in the multi-category medicine box enters the four-way pipe through the feed pipe and the one-way valve 1, and finally reaches the syringe barrel. Then, the piston 1 is closed, and the electric telescopic rod contracts to return to the initial position, and the automatic replenishment of the medicine can be completed.

[0030] 2. In the utility model, the distilled water enters the four-way pipe through the cleaning water pipe and the distribution pipe for flushing. The heating ring and the fan are started to dry the inside of the converging box with hot air, and the air is filtered through the filter screen. The moisture is absorbed by the sponge, and the dry air is discharged, so that the pipeline after operation can be cleaned and dried to prevent the influence of medicine residue on the use. Description of the Drawings

[0031] Figure 1 It is a three-dimensional view of the precise perfusion drug delivery device for patch clamp experiments proposed by the utility model;

[0032] Figure 2 It is a front view of the precise perfusion drug delivery device for patch clamp experiments proposed by the utility model;

[0033] Figure 3Top view of the precision perfusion drug delivery device for patch clamp experiments proposed by the present utility model;

[0034] Figure 4 Rear view of the precision perfusion drug delivery device for patch clamp experiments proposed by the present utility model;

[0035] Figure 5 Cross-sectional view of the convergence box of the precision perfusion drug delivery device for patch clamp experiments proposed by the present utility model.

[0036] Legend description:

[0037] 1. Equipment base; 2. Cleaning mechanism; 201. Hollow groove; 202. Heating ring; 203. Fan; 204. Air inlet hole; 205. Filter sponge; 206. Exhaust baffle; 207. Filter net; 208. Cleaning water pipe; 209. Water pipe valve; 210. Liquid separation pipe; 3. Fixed column; 4. Fixed plate; 5. Electric telescopic rod; 6. Concave clamping plate; 7. Limit clamping plate; 8. Syringe; 9. Piston rod; 10. Piston I; 11. Multi-class medicine box; 12. Four-way pipe; 13. Check valve I; 14. Feed pipe; 15. Check valve II; 16. Convergence box; 17. Conical liquid discharge block; 18. Piston II; 19. Liquid adding pipe; 20. Lamp post; 21. Lighting lamp; 22. Alarm; 23. Notch; 24. Indicator light; 25. Light sensor; 26. Laser emitter; 27. Observation window; 28. Sealing frame; 29. Scale line; 30. Anti-slip net block; 31. Limit baffle; 32. Clamping block. Detailed implementation manners

[0038] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0039] Refer to Figure 1 、 Figure 2 and Figure 3, An embodiment provided by the present utility model: A precision perfusion drug delivery device for patch clamp experiments, including a device base 1. In the middle of the top of the device base 1, a fixed column 3 is fixedly connected. At the rear side of the top outer wall of the fixed column 3, a fixing plate 4 is fixedly connected. On the left and right sides of the rear part of the top of the fixing plate 4, electric telescopic rods 5 are fixedly connected. At one end of the two electric telescopic rods 5, a concave clamping plate 6 is fixedly connected. At the front side of the top outer wall of the fixed column 3, a limit clamping plate 7 is fixedly connected. Inside the inner wall of the limit clamping plate 7, there are multiple syringe barrels 8. Inside the inner wall of the syringe barrel 8, there is a plunger rod 9. The top outer walls of the multiple plunger rods 9 are engaged with the concave clamping plate 6. At the bottom of the syringe barrel 8, a piston one 10 is fixedly connected. At the bottom of the piston one 10, a four-way pipe 12 is fixedly connected. At the rear side of the four-way pipe 12, a one-way valve one 13 is connected. At the rear side of the top of the device base 1, multiple types of medicine boxes 11 are fixedly connected. At the rear side of the one-way valve one 13, a feed pipe 14 is connected. The bottoms of the multiple feed pipes 14 are connected to the multiple types of medicine boxes 11. At the bottom of the four-way pipe 12, a one-way valve two 15 is connected. The bottoms of the multiple one-way valve two 15 are connected to a converging box 16. At the bottom of the converging box 16, there is a liquid adding component. Inside the converging box 16, there is a cleaning mechanism 2. The cleaning mechanism 2 is used to clean the pipeline to prevent bacterial infection;

[0040] Specifically, when it is necessary to add medicine to the tissue sample, just open the piston one 10 above the required medicine. At this time, due to the gravity of the liquid medicine itself, the liquid medicine will enter the converging box 16 through the piston one 10 and the one-way valve two 15, and the liquid medicine is added to the tissue sample through the liquid adding component. When the medicine in the syringe barrel 8 is used up or needs to be replenished, at this time, start the electric telescopic rod 5, which can drive the concave clamping plate 6 to move towards the top of the device base 1, and then drive the plunger rod 9 to move upwards. At this time, suction will be generated in the syringe barrel 8, so that the corresponding type of medicine in the multiple types of medicine boxes 11 is sucked into the feed pipe 14, enters the four-way pipe 12 through the one-way valve one 13, and finally enters the syringe barrel 8, thus completing the automatic addition of the liquid medicine. Then close the piston one 10. At this time, the electric telescopic rod 5 can be started to contract and return to the initial position.

[0041] Refer to Figure 2 、 Figure 4 and Figure 5The cleaning mechanism 2 includes two hollow grooves 201, which are arranged on the left and right sides of the inner wall of the convergence box 16. A heating ring 202 is fixedly connected to the left side of the inner wall of the right hollow groove 201, and a fan 203 is fixedly connected to the right side of the inner wall of the right hollow groove 201. A plurality of air inlet holes 204 are arranged on the right side of the rear of the convergence box 16, and a filter screen 207 is fixedly connected to the rear side of the convergence box 16. A filter sponge 205 is arranged on the inner wall of the left hollow groove 201, and an exhaust baffle 206 is arranged on the left side of the top of the convergence box 16. The outer wall of the exhaust baffle 206 penetrates the convergence box 16, and a cleaning water pipe 208 is arranged on the top of the convergence box 16. The outer wall of the cleaning water pipe 208 is connected to a plurality of liquid distribution pipes 210. The hollow grooves 201 are respectively connected to the front sides of the corresponding four-way pipes 12, and a water pipe valve 209 is fixedly connected to the right side of the outer wall of the cleaning water pipe 208.

[0042] Specifically, after the liquid addition is completed, the distilled water from the outside is guided through the cleaning water pipe 208, and the upper water pipe valve 209 is opened to allow the distilled water to enter the four-way pipe 12 through the liquid distribution pipe 210, thereby flushing the four-way pipe 12, the one-way valve 15, the gathering box 16 and the liquid addition component to prevent the residual medicine from affecting the next use. Then, the heating ring 202 and the fan 203 are started to blow dry hot air into the interior of the gathering box 16, thereby drying the interior, and the outside air will be filtered by the filter net 207, and the moisture inside the gathering box 16 will be driven to the top of the filter sponge 205 to absorb the moisture, and the air will pass through the filter sponge 205 and be discharged through the exhaust baffle 206, thereby completing the cleaning and drying of the internal pipeline.

[0043] Reference Figure 1 , Figure 4 and Figure 5 The liquid adding component includes a conical liquid discharge block 17, the conical liquid discharge block 17 is connected to the bottom of the convergence box 16, the bottom of the conical liquid discharge block 17 is fixedly connected to a piston 2 18, the bottom of the piston 2 18 is connected to a liquid adding pipe 19, a lamp post 20 is fixedly connected to the left side of the top of the equipment seat 1, a lighting lamp 21 is fixedly connected to the right side of the lamp post 20, an alarm 22 is fixedly connected to the left rear side of the top of the equipment seat 1, a notch 23 is provided at the top of the front end of the exhaust baffle 206, a plurality of indicator lights 24 are fixedly connected to the front side of the outer wall of the convergence box 16, a plurality of laser emitters 26 are fixedly connected to the rear side of the inner wall of the convergence box 16, and a plurality of photosensitive sensors 25 are fixedly connected to the front side of the inner wall of the convergence box 16;

[0044] Specifically, the liquid accumulates through the conical liquid discharge block 17. By opening the second piston 18, the liquid can pass through and is filled through the liquid filling pipe 19. The visibility of the experimental operation can be improved through the lighting lamp 21, which facilitates the experimental personnel to connect pipes and adjust valve operations. The alarm 22 can give an alarm in case of a failure. The notch 23 facilitates the extraction of the exhaust baffle 206. The laser emitter 26 emits light. When there is liquid flowing through, the light will be refracted, so that it cannot be received by the light sensor 25. At this time, the indicator light 24 will light up, indicating that there is liquid flowing through the pipe at this place.

[0045] Referring to Figure 2 and Figure 3 On the front side of the outer wall of the multi-class medicine box 11, an observation window 27 is opened. A sealing frame 28 is fixedly connected to the outside of the observation window 27. A plurality of scale lines 29 are opened on the outer wall of the syringe barrel 8. An anti-slip net block 30 is fixedly connected to the top of the plunger rod 9. A plurality of limit baffles 31 are rotatably connected to the top of the front side of the limit clamping plate 7. A plurality of clamping blocks 32 are fixedly connected to the middle of the front side of the limit clamping plate 7. The limit baffles 31 are engaged with the clamping blocks 32.

[0046] Specifically, through the observation window 27, the remaining situation of each medicine in the multi-class medicine box 11 can be known. Through the scale lines 29, the volume drawn into the syringe barrel 8 can be known. Through the anti-slip net block 30, the anti-slip ability of the plunger rod 9 can be improved. The engagement of the limit baffles 31 and the clamping blocks 32 can prevent the syringe barrel 8 from falling.

[0047] Working principle: Before using this device, first, when applying medicine to the tissue sample, open the first piston 10 above the required medicine. The liquid medicine will flow into the converging box 16 through the first piston 10 and the second one-way valve 15 by virtue of its own gravity, and apply the medicine to the tissue sample through the liquid adding assembly. When the medicine in the syringe barrel 8 is applied completely or needs to be replenished, start the electric telescopic rod 5, which can move the concave clamping plate 6 and the plug rod 9 towards the top of the equipment base 1, causing suction in the syringe barrel 8, so that the corresponding type of medicine is sucked from the multi-category medicine boxes 11 into the feed pipe 14, flows into the four-way pipe 12 through the first one-way valve 13, and finally enters the syringe barrel 8, thus realizing the automatic addition of medicine. After the addition is completed, close the first piston 10 and start the contraction of the electric telescopic rod 5 to make it return to the initial position. And through the cleaning mechanism 2, when the liquid adding operation is completed, guide external distilled water in through the cleaning water pipe 208. By opening the upper water pipe valve 209, the distilled water flows into the four-way pipe 12 through the liquid distribution pipe 210, so as to flush the four-way pipe 12, the second one-way valve 15, the converging box 16 and the liquid adding assembly, to avoid the adverse impact of residual medicine on subsequent operations. Subsequently, start the heating ring 202 and the blower 203, send dry hot air into the interior of the converging box 16 for drying treatment. At the same time, the outside air will be filtered by the filter screen 207, and the moisture inside the converging box 16 is guided above the filter sponge 205, the moisture therein is absorbed, and the air is discharged through the filter sponge 205 and the exhaust baffle 206, thus realizing the cleaning and drying of the internal pipeline.

[0048] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacement of some technical features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A precision perfusion drug delivery device for patch clamp experiments, comprising a device base (1), characterized in that: A fixing column (3) is fixedly connected to the middle of the top of the equipment seat (1); a fixing plate (4) is fixedly connected to the rear side of the top of the outer wall of the fixing column (3); electric telescopic rods (5) are fixedly connected to the left and right sides of the rear of the top of the fixing plate (4); one end of the two electric telescopic rods (5) is fixedly connected to a concave clamping plate (6); a limit clamping plate (7) is fixedly connected to the front side of the top of the outer wall of the fixing column (3); a plurality of injection cylinders (8) are provided on the inner wall of the limit clamping plate (7); a plug rod (9) is provided on the inner wall of the injection cylinder (8); the top of the outer wall of the plurality of plug rods (9) is engaged with the concave clamping plate (6); a piston 1 (10) is fixedly connected to the bottom of the injection cylinder (8); the piston 1 (10) The bottom of the equipment base (10) is fixedly connected to a four-way pipe (12), the rear side of the four-way pipe (12) is connected to a one-way valve (13), the top rear side of the equipment base (1) is fixedly connected to multiple types of medicine boxes (11), the rear side of the one-way valve (13) is connected to a feed pipe (14), the bottoms of multiple feed pipes (14) are connected to the multiple types of medicine boxes (11), the bottom of the four-way pipe (12) is connected to a one-way valve (15), the bottoms of multiple one-way valves (15) are connected to a convergence box (16), the bottom of the convergence box (16) is provided with a liquid adding component, the interior of the convergence box (16) is provided with a cleaning mechanism (2), and the cleaning mechanism (2) is used to clean the pipeline to prevent bacterial infection.

2. The precision perfusion drug delivery device for patch clamp experiments according to claim 1, characterized in that: The cleaning mechanism (2) comprises two hollow grooves (201), the two hollow grooves (201) being arranged on the left and right sides of the interior of the convergence box (16), a heating ring (202) being fixedly connected to the left side of the inner wall of the right hollow groove (201), a fan (203) being fixedly connected to the right side of the inner wall of the right hollow groove (201), a plurality of air inlet holes (204) being arranged on the right side of the rear portion of the convergence box (16), a filter screen (207) being fixedly connected to the rear side of the convergence box (16), and a filter screen (207) being fixedly connected to the left side of the hollow groove (201). A filter sponge (205) is arranged on the inner wall, an exhaust baffle (206) is arranged on the left side of the top of the convergence box (16), the outer wall of the exhaust baffle (206) passes through the convergence box (16), a cleaning water pipe (208) is arranged on the top of the convergence box (16), the outer wall of the cleaning water pipe (208) is connected to a plurality of liquid distribution pipes (210), the hollow grooves (201) are respectively connected to the front sides of the corresponding four-way pipes (12), and a water pipe valve (209) is fixedly connected to the right side of the outer wall of the cleaning water pipe (208).

3. The precision perfusion drug delivery device for patch clamp experiments according to claim 1, characterized in that: The liquid adding assembly comprises a conical liquid draining block (17), the conical liquid draining block (17) being connected to the bottom of the convergence box (16), a second piston (18) being fixedly connected to the bottom of the conical liquid draining block (17), and a liquid adding pipe (19) being connected to the bottom of the second piston (18).

4. The precision perfusion drug delivery device for patch clamp experiments according to claim 2, characterized in that: A lamp post (20) is fixedly connected to the left side of the top of the equipment seat (1), a lighting lamp (21) is fixedly connected to the right side of the lamp post (20), an alarm (22) is fixedly connected to the rear side of the left portion of the top of the equipment seat (1), and a notch (23) is provided at the top of the front end of the exhaust baffle (206).

5. The precision perfusion drug delivery device for patch clamp experiments according to claim 1, characterized in that: A plurality of indicator lights (24) are fixedly connected to the front side of the outer wall of the convergence box (16), a plurality of laser emitters (26) are fixedly connected to the rear side of the inner wall of the convergence box (16), and a plurality of light sensors (25) are fixedly connected to the front side of the inner wall of the convergence box (16).

6. The precision perfusion drug delivery device for patch clamp experiments according to claim 1, characterized in that: An observation window (27) is provided on the front side of the outer wall of the multi-type medicine box (11), and a sealing frame (28) is fixedly connected to the outer side of the observation window (27).

7. The precision perfusion drug delivery device for patch clamp experiments according to claim 1, characterized in that: The outer wall of the injection barrel (8) is provided with a plurality of scale marks (29), and the top of the plug rod (9) is fixedly connected with an anti-slip mesh block (30).

8. The precision perfusion drug delivery device for patch clamp experiments according to claim 1, characterized in that: A plurality of limit baffles (31) are rotatably connected to the top of the front side of the limit clamping plate (7), a plurality of clamping blocks (32) are fixedly connected to the middle of the front side of the limit clamping plate (7), and the limit baffles (31) are engaged with the clamping blocks (32).

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