Enzymatic hydrolysate centrifugal separation and purification equipment for active peptide powder processing

Abstract

The utility model discloses enzymatic hydrolysate centrifugal separation and purification equipment for active peptide powder processing, and relates to the technical field of active peptide powder processing, the enzymatic hydrolysate centrifugal separation and purification equipment comprises a centrifugal machine, a tank body, a finished product tank, a liquid outlet pipe, a connecting pipe and a filter box, the lower part of the centrifugal machine is communicated with the finished product tank, the tank body is arranged at one side of the finished product tank, and the liquid outlet pipe is arranged at the other side of the finished product tank; the centrifugal machine is communicated with the finished product tank through a pipe body, and the centrifugal machine is communicated with the finished product tank through the liquid outlet pipe. According to the utility model, enzymatic hydrolysate is primarily purified through the centrifugal machine, solid particles (such as cell debris and unreacted substrates) in the enzymatic hydrolysate are separated from liquid, and the separated finished product liquid directly enters the filter box; the finished product liquid sequentially passes through the arc-shaped filter screen, the filter plate I and the filter plate II to perform membrane filtration on small molecular impurities and part of macromolecular substances, and the centrifuged finished product liquid is refined, so that centrifugation and membrane filtration are performed simultaneously, and the production efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of active peptide powder processing, and specifically relates to an enzymatic hydrolysate centrifugal separation and purification device for active peptide powder processing. Background Technique

[0002] At present, in the process of processing bioactive peptide powder, the treatment of enzymatic hydrolysate is a key step. The enzymatic hydrolysate usually contains substances with various molecular weights, including target active peptides, unreacted substrates, enzymes, cell debris, and other impurities. In order to obtain high-purity active peptides, effective separation and purification of the enzymatic hydrolysate are required.

[0003] Traditional separation and purification methods often first perform centrifugation on the enzymatic hydrolysate through a centrifuge to separate the solid particles (such as cell debris and unreacted substrates) in the enzymatic hydrolysate from the liquid, thereby achieving preliminary purification. However, centrifugal separation cannot remove all small-molecule impurities and some macromolecular substances. Therefore, membrane filtration technology needs to be further used to refine the centrifuged liquid to form the final purified liquid. At present, centrifugation and filtration are carried out separately, which is not only cumbersome but also has low production efficiency. For this reason, an enzymatic hydrolysate centrifugal separation and purification device for active peptide powder processing is proposed. Content of the Utility Model

[0004] To solve the above problems, that is, to solve the problems proposed in the above background technique, the utility model proposes an enzymatic hydrolysate centrifugal separation and purification device for active peptide powder processing, which includes a centrifuge, a tank body, a finished product tank, a liquid outlet pipe, a connecting pipe, and a filtration box. The lower part of the centrifuge is communicated with the finished product tank. The tank body is arranged on one side of the finished product tank. The centrifuge and the finished product tank are communicated through a pipe body. The centrifuge and the finished product tank are communicated through the liquid outlet pipe. The finished product tank and the filtration box are communicated through the connecting pipe. A sealed movable door is arranged at the upper end of the finished product tank, and a filtration structure is installed in the finished product tank;

[0005] The filtration structure includes a pair of clamping plates, a pair of U-shaped plates I, a pair of U-shaped plates II, an arc-shaped filter screen, a filter plate I, and a filter plate II;

[0006] The filtration box is a circular box body. At one end of the filtration box close to the centrifuge, a pair of the clamping plates, a pair of the U-shaped plates I, and a pair of the U-shaped plates II are respectively fixedly connected. The arc-shaped filter screen is slidably clamped between the pair of clamping plates and the filtration box. The two ends of the filter plate I and the filter plate II are respectively fixedly connected with corresponding sliding rods. The pair of sliding rods of the filter plate I are respectively slidably arranged in the corresponding chutes of the U-shaped plates I, and the pair of sliding rods of the filter plate II are respectively slidably arranged in the corresponding chutes of the U-shaped plates II.

[0007] The utility model is further configured as follows: the filter plate 1 and the filter plate 2 are both composed of a rectangular frame and a filter membrane.

[0008] The utility model is further configured as follows: the arc-shaped filter screen is composed of an arc-shaped shell and a filter membrane.

[0009] The utility model is further configured as follows: the tank body is a large particle storage tank.

[0010] The utility model is further configured as follows: the filter plate 1, the arc filter screen and the filter plate 2 are all detachable plates.

[0011] The utility model is further configured as follows: the filter membrane of the arc-shaped filter screen is a microfiltration membrane, which can intercept particles between 0.1 and 1 micron and allow macromolecules and soluble solids to pass through.

[0012] The utility model is further configured as follows: the filter membrane of the filter plate 1 is an ultrafiltration membrane, and the molecular weight cut-off (MWCO) can reach 10,000Da, which is used to remove bacteria, viruses and solids.

[0013] The utility model is further configured as follows: the filter membrane of the filter plate 2 is a nanofiltration membrane with a cut-off molecular weight of 1,000 Da, which is capable of retaining relatively large molecules.

[0014] The beneficial technical effect of the utility model is as follows: the utility model first performs preliminary purification of the enzymatic hydrolyzate through a centrifuge, separates the solid particles (such as cell fragments and unreacted substrate) in the enzymatic hydrolyzate from the liquid, and the finished liquid after separation directly enters the filter box, and the finished liquid passes through the arc filter, filter plate one and filter plate two in turn to perform membrane filtration on small molecular impurities and part of macromolecular substances, and refines the finished liquid after centrifugation, so as to achieve simultaneous centrifugation and membrane filtration, thereby improving production efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 Shown is a front view of the present utility model.

[0016] Figure 2 A side view of the present invention is shown.

[0017] Figure 3 A top view of the present invention is shown.

[0018] Figure numerals 1, centrifuge, 2, tank body, 3, finished product tank, 4, liquid outlet pipe, 5, connecting pipe, 6, filter box, 7, U-shaped plate 2, 8, U-shaped plate 1, 9, clamping plate, 10, arc filter screen, 11, filter plate 1, 12, filter plate 2. DETAILED DESCRIPTION

[0019] Please refer to the attached Figures 1 - 3The preferred embodiments of the present utility model will be described. Those skilled in the art should understand that these embodiments are only used to explain the technical principle of the present utility model and are not intended to limit the protection scope of the present utility model.

[0020] The present utility model provides a centrifugal separation and purification device for enzymatic hydrolysate in the processing of active peptide powder. When using this device, open the sealed movable door of the filtration box 6. First, slide and snap the arc-shaped filter screen 10 between a pair of clamping plates 9 and the inner wall of the filtration box 6. Then, snap the first filter plate 11 into the chute of the corresponding first U-shaped plate 8 through the corresponding slide rod. Next, snap the second filter plate 12 into the chute of the corresponding second U-shaped plate 7 through the corresponding slide rod. Close the movable seal cover, and introduce the enzymatic hydrolysate into the centrifuge 1. The centrifuge 1 operates to purify the enzymatic hydrolysate for the first time. The large particles generated during centrifugation fall into the tank body 2, and the finished product liquid generated during centrifugation flows into the finished product tank 3 through the liquid outlet pipe 4. The finished product liquid in the finished product tank 3 flows into the filtration box 6 through the connecting pipe 5. The finished product liquid flowing into the filtration box 6 sequentially passes through the arc-shaped filter screen 10, the first filter plate 11, and the second filter plate 12. The finished product is sequentially subjected to small particle filtration, bacteria and virus filtration, and macromolecule filtration through the microfiltration membrane, ultrafiltration membrane, and nanofiltration membrane, refining the centrifuged finished product liquid, and performing secondary purification treatment on the finished product liquid, achieving the purpose of simultaneous centrifugation and membrane filtration, thereby improving production efficiency. Since the arc-shaped filter screen 10, the first filter plate 11, and the second filter plate 12 are all detachable filter plates, the movable seal tank of the filtration box 6 can be opened regularly to replace the arc-shaped filter screen 10, the first filter plate 11, and the second filter plate 12.

[0021] Although the present utility model has been described with reference to the preferred embodiments, various improvements can be made to it and components therein can be replaced with equivalents without departing from the scope of the present utility model. In particular, as long as there is no structural conflict, the various technical features mentioned in each embodiment can be combined in any way. The present utility model is not limited to the specific embodiments disclosed in the text but includes all technical solutions falling within the scope of the claims.

[0022] In the description of the present utility model, the terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., which indicate directions or position relationships, are based on the directions or position relationships shown in the drawings. This is only for the convenience of description and does not indicate or imply that the device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus cannot be construed as a limitation of the present utility model. In addition, the terms "first", "second", "third" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance.

[0023] In addition, it should be noted that in the description of the present utility model, unless otherwise clearly specified and defined, the terms "installed", "connected", and "coupled" should be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or an integral connection; it may be a mechanical connection or an electrical connection; it may be directly connected or indirectly connected through an intermediate medium, and it may be the communication inside two elements. For those skilled in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0024] The term "comprising" or any other similar term is intended to cover non-exclusive inclusion, so that a process, article, or apparatus / device comprising a series of elements includes not only those elements but also other elements not expressly listed, or also includes elements inherent in these processes, articles, or apparatus / devices.

[0025] So far, the technical solutions of the present utility model have been described in conjunction with the preferred embodiments shown in the drawings. However, it is easy for those skilled in the art to understand that the protection scope of the present utility model is obviously not limited to these specific embodiments. Without departing from the principle of the present utility model, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will fall within the protection scope of the present utility model.

Claims

1. A centrifugal separation and purification device for enzymatic hydrolysate for active peptide powder processing, comprising a centrifuge (1), a tank body (2), a finished product tank (3), a liquid outlet pipe (4), a connecting pipe (5) and a filter box (6), characterized in that: The lower part of the centrifuge (1) is connected to the finished product tank (3), the tank body (2) is arranged on one side of the finished product tank (3), the centrifuge (1) and the finished product tank (3) are connected through a pipe body, the centrifuge (1) and the finished product tank (3) are connected through the liquid outlet pipe (4), the finished product tank (3) and the filter box (6) are connected through the connecting pipe (5), the upper end of the finished product tank (3) is provided with a sealed movable door, and a filtering structure is installed in the finished product tank (3); The filtering structure comprises a pair of clamping plates (9), a pair of U-shaped plates 1 (8), a pair of U-shaped plates 2 (7), an arc-shaped filter screen (10), a filter plate 1 (11) and a filter plate 2 (12); The filter box (6) is a circular box body, and one end of the filter box (6) close to the centrifuge (1) is fixedly connected to a pair of the clamping plates (9), a pair of the U-shaped plates (8) and a pair of the U-shaped plates (7). The arc filter (10) is slidably clamped between the pair of the clamping plates (9) and the filter box (6). The two ends of the filter plate (11) and the filter plate (12) are fixedly connected to corresponding sliding rods. The pair of sliding rods of the filter plate (11) are slidably arranged in the corresponding sliding grooves of the U-shaped plate (8), and the pair of sliding rods of the filter plate (12) are slidably arranged in the corresponding sliding grooves of the U-shaped plate (7).

2. The centrifugal separation and purification equipment for enzymatic hydrolysate for active peptide powder processing according to claim 1, characterized in that: The filter plate 1 (11) and the filter plate 2 (12) are both composed of a rectangular frame and a filter membrane.

3. The centrifugal separation and purification equipment for enzymatic hydrolysate for active peptide powder processing according to claim 1, characterized in that: The arc-shaped filter screen (10) is composed of an arc-shaped shell and a filter membrane.

4. The centrifugal separation and purification equipment for enzymatic hydrolysate for active peptide powder processing according to claim 1, characterized in that: The tank body (2) is a large particle storage tank.

5. The centrifugal separation and purification equipment for enzymatic hydrolysate for active peptide powder processing according to claim 1, characterized in that: The filter plate 1 (11), the arc-shaped filter screen (10) and the filter plate 2 (12) are all detachable plates.

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