Hymenoptera insect smell selectivity experiment device

Abstract

The utility model relates to a hymenoptera insect smell selectivity experiment device, which belongs to the field of biological science experiment instruments and comprises a movable search box, a transparent top cover is movably attached to the top end of the movable search box, and an experiment mechanism is arranged in the movable search box. According to the hymenoptera insect smell selectivity experiment device, a tested smell source is placed in the two selection chambers respectively, the transparent top cover is quickly screwed after the smell source is placed in, the straight opening end of the inlet pipe and the connecting opening are fixed, tested insects are released, after the tested insects enter the movable search box, selection can be carried out according to the smell emitted by the selection chambers, and then the tested insects can be selected according to the smell emitted by the selection chambers. After being selected according to preferences, the test insects enter through the small ventilation holes, and finally, data such as the number of the test insects entering the small selection chamber or the selection time of a single test insect in the set time are counted, so that the purpose of conveniently carrying out multiple groups of experiments is achieved, and the problems that multiple groups of experiments are inconvenient to carry out at the same time and the actual operation cost is high are solved.

Description

Technical Field

[0001] The utility model relates to the technical field of biological science experimental instruments, in particular to a device for odor selectivity experiments of hymenoptera insects. Background Technique

[0002] Parasitic hymenoptera insects, parasitic wasps, are a very important type of natural enemy insects and play a crucial role in the natural control of pests and the biological control of agriculture and forestry. In recent decades, domestic and foreign scholars have carried out many scientific researches on the biological characteristics of parasitic wasps, the interaction relationship with host plants, the exploration and development and utilization of potential parasitic wasp biological control resources, etc. With the continuous increase in the breadth and depth of research, the ecological value and status of parasitic wasps have gradually increased. In the natural environment, parasitic wasps need to go through three stages to find hosts: habitat location, host location, and host recognition. During the search process, parasitic wasps first locate the host position by olfactory recognition of various complex physical and chemical signals, and then carry out parasitic behaviors. Precise host location and successful parasitism determine the survival and reproduction of the parasitic wasp population. Selectivity experiments are a test method to explore the search behavior, efficiency, and competition relationship of target parasitic wasps, and are used to evaluate the effects of different hosts and different plants on the searching ability and parasitic ability of parasitic wasps, as well as the competition relationship between different species of parasitic wasps on the same host. Exploring the relationship between parasitic wasps and the volatile odor substances related to their selection behaviors will help improve the control efficiency of target pests. Understanding the competition relationship and competition effect among parasitic wasps on the control efficiency of pests will provide a basis for pest biological control.

[0003] Most selectivity experiments are carried out in glass experimental test tubes. The insect experimental device is relatively complex, usually composed of a gas collection device and a glass tube and requires power connection. In addition, it is not convenient to carry out multiple groups of experiments simultaneously, and the actual operation cost is relatively high. Therefore, this application proposes a device for odor selectivity experiments of hymenoptera insects. Content of the Utility Model

[0004] Aiming at the deficiencies of the prior art, the utility model provides a device for odor selectivity experiments of hymenoptera insects, which has the advantage of being convenient to carry out multiple groups of experiments, and solves the problems of inconvenience in simultaneously carrying out multiple groups of experiments and relatively high actual operation cost.

[0005] To achieve the above purpose, the utility model provides the following technical solution: A device for odor selectivity experiments of hymenoptera insects, including a movable search box, a transparent top cover is movably attached to the top of the movable search box, and an experimental mechanism is arranged inside the movable search box;

[0006] The experimental mechanism includes a classification component and a feeding component. The classification component includes two selection chambers fixed on the inner bottom wall of the movable search box, and a plurality of air-permeable small holes are opened on the outer sides of the two selection chambers;

[0007] The feeding assembly includes the straight mouth end of the inlet pipe fixed to the front side of the movable search box. The front side of the straight mouth end of the inlet pipe is fixedly and communicatively connected with the middle section hose of the inlet pipe. The front side of the middle section hose of the inlet pipe is fixedly and communicatively connected with the flared mouth end of the inlet pipe. The front side of the flared mouth end of the inlet pipe is fixedly and communicatively connected with the Dufour's gland duct.

[0008] Further, sealing rubber rings that are hermetically and movably attached to the inner top wall of the transparent top cover are fixed to the tops of both of the selection chambers.

[0009] Further, the movable search box and the two selection chambers are both cylindrical bodies that are circular in shape and have a missing upper surface.

[0010] Further, a connection port communicating with the straight mouth end of the inlet pipe is provided at the center of the front side of the movable search box.

[0011] Further, both of the selection chambers are covering layers, and the covering layer is an acrylic layer.

[0012] Further, external threads are provided on the outer side of the movable search box and near its top.

[0013] Further, internal threads that are threadedly connected to the external threads are provided on the inner wall of the inner cavity of the transparent top cover and near its bottom end.

[0014] Compared with the prior art, the technical solution of the present application has the following beneficial effects:

[0015] For the device for the odor selectivity experiment of hymenopteran insects, the tested odor sources are respectively placed inside the two selection chambers. After the odor sources are placed, the transparent top cover is quickly tightened, the straight mouth end of the inlet pipe and the connection port are fixed, and the test insects are released. When the test insects enter the movable search box, they will make a choice according to the odor emitted by the selection chambers. After making a choice according to their preference, they enter through the breathable small holes. Finally, data such as the number of test insects entering the selection chamber within a specified time or the time taken for a single test insect to make a choice are counted, achieving the purpose of facilitating the conduct of multiple groups of experiments and solving the problems of inconvenience in simultaneously conducting multiple groups of experiments and relatively high actual operation costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 is a three-dimensional view of the structure of the present utility model;

[0017] Figure 2 is a cross-sectional view of the structure of the present utility model;

[0018] Figure 3 is a top view of the structure of the straight mouth end of the inlet pipe of the present utility model.

[0019] In the figure: 1 is a movable search box, 2 is an experimental mechanism, 201 is a selection chamber, 202 is a sealing rubber ring, 203 is a breathable small hole, 204 is the straight end of the inlet pipe, 205 is the middle hose of the inlet pipe, 206 is the flared end of the inlet pipe, 207 is a ductulus efferens, and 3 is a transparent top cover. Specific implementation mode

[0020] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0021] Please refer to Figure 1 , a device for an odor selectivity experiment of hymenopteran insects in this embodiment, includes a movable search box 1. A transparent top cover 3 is movably attached to the top end of the movable search box 1, and an experimental mechanism 2 is arranged inside the movable search box 1.

[0022] An external thread is provided on the outer side of the movable search box 1 and near its top, and an internal thread that is threadedly connected to the external thread is provided on the inner wall of the inner cavity of the transparent top cover 3 and near its bottom end.

[0023] It should be noted that the movable search box 1 is set to be circular, which has enough activity space for the test insects to move stably and search. By setting the connection between the movable search box 1 and the transparent top cover 3 as a threaded connection, the airtightness of the movable search box 1 can be ensured, reducing external interference and preventing the test insects from escaping during the experiment. The movable search box 1 is made of frosted light-transmitting and color-impermeable acrylic material. The purpose of being color-impermeable is to reduce the influence of the surrounding colors on the selection behavior of the test insects during the experiment. The transparent top cover 3 is made of transparent acrylic material, which is convenient for experimenters to observe and record. The activity search area provided by the movable search box 1 for the test insects is a non-independent space, avoiding the influence of a specific shape on the search trajectory of the test insects. The test insects can fully contact the odor in a sufficient activity space and locate the preferred odor source.

[0024] Please refer to Figures 2 to 3, To facilitate the conduct of multiple groups of experiments, the experimental mechanism 2 in this embodiment includes a classification component and a feeding component. The classification component includes two selection chambers 201 fixed to the inner bottom wall of the movable search box 1. A plurality of air-permeable small holes 203 are provided on the outer sides of the two selection chambers 201. The feeding component includes an inlet pipe straight port end 204 fixed to the front side of the movable search box 1. The front side of the inlet pipe straight port end 204 is fixedly connected to an inlet pipe middle section hose 205. The front side of the inlet pipe middle section hose 205 is fixedly connected to an inlet pipe flared port end 206. The front side of the inlet pipe flared port end 206 is fixedly connected to a ductulus efferens 207. When the test insects can fully contact the odor in a sufficient activity space, locate the preferred odor source, and enter the selection chamber 201 through the plurality of air-permeable small holes 203.

[0025] In this example, the movable search box 1 and the two selection chambers 201 are both circular disk-shaped cylinders with the upper surface missing. The function of setting the selection chamber 201 is to provide a fixed point position for placing the odor source to be tested, ensuring the consistency of each repeated experiment. Another function is to separate the movable search box 1. The concentration of the odor emitted outward in the selection chamber 201 is different. Sealing rubber rings 202 that are hermetically and movably fitted to the inner top wall of the transparent top cover 3 are fixed to the tops of the two selection chambers 201. A connection port communicating with the inlet pipe straight port end 204 is provided at the center of the front side of the movable search box 1. The inlet pipe straight port end 204 ensures the stability of the connection with the connection port. The inlet pipe middle section hose 205 serves to make the inlet pipe flared port end 206 hang naturally, creating a height difference to enable the test insects to crawl upwards quickly and enter the interior of the movable search box 1. The two selection chambers 201 are both covering layers, and the covering layer is an acrylic layer. The odor sources to be tested are respectively placed inside the two selection chambers 201. After the odor sources are placed, the transparent top cover 3 is quickly tightened, the inlet pipe straight port end 204 and the connection port are fixed, the ductulus efferens 207 is connected to the inlet pipe flared port end 206, and the test insects are released. After the test insects enter the movable search box 1 through the inlet pipe flared port end 206, the inlet pipe middle section hose 205, the inlet pipe straight port end 204, and the connection port, the experiment starts. When the test insects enter the movable search box 1, they will make a choice according to the odor emitted by the selection chamber 201, enter through the air-permeable small holes 203 after making a preference selection, and finally count data such as the number of test insects entering the selection chamber 201 within a specified time or the time taken for a single test insect to make a choice.

[0026] It should be noted that the odor sources to be tested are respectively placed inside the two selection chambers 201. After the odor sources are placed, quickly tighten the transparent top cover 3. Fix the straight end 204 of the inlet pipe and the connection port. Release the test insects. When the test insects enter the activity search box 1, they will make a choice according to the odor emitted by the selection chamber 201. After making a choice according to their preference, they enter through the air-permeable small holes 203. Finally, count the number of test insects entering the selection chamber 201 within a specified time or the time taken by a single test insect to make a choice and other data, achieving the purpose of facilitating the conduct of multiple groups of experiments and solving the problems of inconvenience in simultaneously conducting multiple groups of experiments and relatively high actual operation costs.

[0027] It can be understood that in the description of the present utility model, it should be noted that unless otherwise clearly specified and limited, the terms "installation", "connection", and "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations. Where the present utility model is not elaborated, it is all well-known techniques in the art.

[0028] The working principle of the above embodiment is as follows:

[0029] (1) The activity search box 1 is set to be circular, providing enough activity space for the test insects to move stably and search. By setting a threaded connection between the activity search box 1 and the transparent top cover 3, the airtightness of the activity search box 1 can be ensured, reducing external interference and preventing the test insects from escaping during the experiment. The activity search box 1 is made of frosted light-transmitting and color-impermeable acrylic material. The purpose of being color-impermeable is to reduce the influence of surrounding colors on the selection behavior of the test insects during the experiment. The transparent top cover 3 is made of transparent acrylic material, facilitating the experimenter to observe and record. The activity search area provided by the activity search box 1 for the test insects is a non-independent space, avoiding the influence of a limited specific shape on the search trajectory of the test insects. The test insects can fully contact the odor in the sufficient activity space, locate the preferred odor source, and enter the selection chamber 201 through multiple air-permeable small holes 203. The function of setting the selection chamber 201 is to provide a fixed point for placing the odor sources to be tested, ensuring the consistency of each repeated experiment. Another function is to separate the activity search box 1. The concentration of the odor emitted outward in the selection chamber 201 is different. The straight end 204 of the inlet pipe ensures the stability of the connection with the connection port. The middle hose 205 of the inlet pipe plays a role in making the flared end 206 of the inlet pipe droop naturally, creating a height difference to make the test insects crawl upward quickly into the interior of the activity search box 1.

[0030] (2) Place the odor sources to be tested inside the two selection chambers 201 respectively. After placing the odor sources, quickly tighten the transparent top cover 3. Fix the straight end 204 of the inlet pipe and the connection port. Connect the Durham tube 207 to the flared end 206 of the inlet pipe, and release the test insects. After the test insects enter the activity search box 1 through the flared end 206 of the inlet pipe, the middle section hose 205 of the inlet pipe, the straight end 204 of the inlet pipe, and the connection port, start the experiment. When the test insects enter the activity search box 1, they will make a choice according to the odor emitted by the selection chamber 201. After making a preference selection, they enter through the air-permeable small holes 203. Finally, count the number of test insects entering the selection chamber 201 within a specified time or data such as the time taken for a single test insect to make a choice.

[0031] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or also includes elements inherent to such process, method, article or device. Without further limitation, an element defined by the statement "comprising an..." does not exclude the existence of additional identical elements in the process, method, article or device comprising the said element.

[0032] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention.

Claims

1. A device for a hymenoptera insect odor selectivity experiment, comprising a movable search box (1), characterized in that: A transparent top cover (3) is movably attached to the top of the movable search box (1), and an experimental mechanism (2) is arranged inside the movable search box (1); The experimental mechanism (2) comprises a classification component and a feeding component, wherein the classification component comprises two selection chambers (201) fixed to the inner bottom wall of the movable search box (1), and the outer sides of the two selection chambers (201) are provided with a plurality of ventilation holes (203); The feed assembly comprises an inlet pipe straight end (204) fixed on the front side of the movable search box (1); the front side of the inlet pipe straight end (204) is fixedly connected to an inlet pipe middle section hose (205); the front side of the inlet pipe middle section hose (205) is fixedly connected to an inlet pipe bell end (206); the front side of the inlet pipe bell end (206) is fixedly connected to a Dulbecco's tubule (207).

2. The device for the odor selectivity experiment of Hymenoptera insects according to claim 1, characterized in that: The top ends of the two selection chambers (201) are both fixed with sealing rubber rings (202) which are sealably fitted with the inner top wall of the transparent top cover (3).

3. The device for the odor selectivity experiment of Hymenoptera insects according to claim 1, characterized in that: The movable search box (1) and the two selection chambers (201) are both circular disc-shaped cylinders with missing upper surfaces.

4. The device for the odor selectivity experiment of Hymenoptera insects according to claim 1, characterized in that: A connection port communicating with the straight end (204) of the inlet pipe is provided at the front side and the center of the movable search box (1).

5. The device for the odor selectivity experiment of Hymenoptera insects according to claim 1, characterized in that: The two selection chambers (201) are both covering layers, and the covering layers are acrylic layers.

6. The device for the odor selectivity experiment of Hymenoptera insects according to claim 1, characterized in that: The movable search box (1) is provided with an external thread on its outer side and near its top.

7. The device for the odor selectivity experiment of Hymenoptera insects according to claim 6, characterized in that: An internal thread which is threadably connected to the external thread is provided on the inner wall of the inner cavity of the transparent top cover (3) near its bottom end.

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