Colloidal gold detection card for bovine hydatid antibody

Abstract

The utility model relates to the technical field of detection devices, and discloses a bovine hydatid antibody colloidal gold detection card which comprises a detection card shell, an embedded device is movably connected in the detection card shell, a fixed buckle is movably connected to the top of the detection card shell, a sample adding hole is formed in the top of the detection card shell, and the embedded device is movably connected with the fixed buckle. An observation window is formed in the top of the detection card shell, a U-shaped limiting block is fixedly connected to the bottom of the embedded device, a fixed bottom plate is fixedly connected to the top of the embedded device, and a sample pad is fixedly connected to the top of the fixed bottom plate; according to the utility model, the sealing ring is arranged, so that the sterile environment and the use safety of a user in the detection process are guaranteed, the tight fit of the sealing ring effectively prevents the invasion of external pollutants, the secondary infection problem is also avoided, and the stability and the comfort of the user in the operation process are improved due to the addition of the anti-skid grains; sample scattering caused by accidental sliding of the detection card is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of detection devices, and more specifically to a colloidal gold detection card for bovine echinococcus antibodies. Background Art

[0002] In animal husbandry, bovine echinococcosis, as a serious zoonotic parasitic disease, is caused by the intermediate host such as cattle and other ruminants being infected by the metacestode of Echinococcus granulosus, posing a great threat to the development of animal husbandry and animal health. This disease not only affects the production performance of animals but may also lead to animal death, thus causing economic losses and food safety problems. Therefore, early, rapid, and accurate diagnosis of bovine echinococcosis is particularly important. Currently, the diagnostic methods for bovine echinococcosis mainly include etiological detection, immunological detection, and molecular biological detection, etc. However, these methods all have certain limitations in practical applications.

[0003] Deficiencies of the prior art: Existing rapid detection means often have problems such as poor specificity, insufficient sensitivity, or poor stability, and it is difficult to meet the requirements of animal husbandry for rapid and accurate diagnosis of bovine echinococcosis. Some detection cards have high requirements for sample processing and require complex pretreatment steps, increasing the complexity of operation and time cost. Summary of the Utility Model

[0004] In order to overcome the above-mentioned defects of the prior art, the utility model provides a colloidal gold detection card for bovine echinococcus antibodies to solve the problems existing in the above-mentioned background art.

[0005] To achieve the above purpose, the utility model provides the following technical solution: A colloidal gold detection card for bovine echinococcus antibodies, including a detection card housing, an embedded device is movably connected inside the detection card housing, a fixed buckle is movably connected to the top of the detection card housing, a sample addition hole is opened at the top of the detection card housing, an observation window is opened at the top of the detection card housing, a U-shaped limiting block is fixedly connected to the bottom of the embedded device, a fixed bottom plate is fixedly connected to the top of the embedded device, and a sample pad is fixedly connected to the top of the fixed bottom plate.

[0006] Further, a slide rail is fixedly connected to the inner wall of the detection card housing, a guide rail groove is opened on the side of the embedded device, and the embedded device is slidably connected to the side of the slide rail.

[0007] Further, a hydrophilic film is opened at the bottom of the sample addition hole, and an anti-slip pattern is fixedly connected to the top of the detection card housing.

[0008] Further, a positioning plate is fixedly connected to the top of the embedded device, and a limiting groove is fixedly connected to the top of the embedded device.

[0009] Furthermore, a sealing ring is fixedly connected to the top of the embedded device, and a limiting bump is fixedly connected to the top of the embedded device.

[0010] Furthermore, a colloidal gold conjugate pad is fixedly connected to the side of the sample pad, a fiber membrane is fixedly connected to the side of the colloidal gold conjugate pad, and a chromatographic membrane is fixedly connected to the side of the fiber membrane.

[0011] Furthermore, a quality control line is parallelly arranged at the top of the fiber membrane, and a detection line is parallelly arranged at the top of the fiber membrane.

[0012] The technical effects and advantages of the present utility model:

[0013] 1. By providing a sealing ring, the present utility model is beneficial to ensuring a sterile environment during the detection process and the safety of users. The tight fit of the sealing ring effectively prevents the intrusion of external pollutants and also avoids the problem of secondary infection.

[0014] 2. By providing anti-slip patterns, the addition of the anti-slip patterns improves the stability and comfort of users during the operation process, and avoids the accidental slipping of the test card and the spilling of the sample.

[0015] 3. By providing a unique embedded device, through the cooperation of buckles, the present utility model is beneficial to ensuring that the test card can maintain good sealing performance during storage, transportation and use, which can effectively prevent the intrusion of external microorganisms and the loss of internal components. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 is a schematic diagram of the overall structure of the present utility model;

[0017] Figure 2 is a schematic diagram of the housing structure of the present utility model;

[0018] Figure 3 is a schematic diagram of the embedded device structure of the present utility model;

[0019] Figure 4 is a schematic diagram of the colloidal gold test strip structure of the present utility model;

[0020] Figure 5 is a schematic diagram of the internal structure of the present utility model.

[0021] The reference numerals are: 1. Detection card housing; 101. Fixed buckle; 102. Sampling hole; 103. Observation window; 104. Anti-slip pattern; 105. Slide rail; 2. Embedded device; 201. U-shaped limit block; 202. Positioning plate; 203. Limit groove; 204. Guide rail groove; 205. Sealing ring; 206. Limit protrusion; 3. Fixed bottom plate; 301. Sample pad; 302. Colloidal gold conjugate pad; 303. Fiber membrane; 3031. Control line; 3032. Test line; 304. Chromatographic membrane. Detailed implementation mode

[0022] Next, the technical solutions in the present invention will be clearly and completely described in conjunction with the accompanying drawings in the present invention. In addition, the forms of each structure described in the following implementation modes are only examples. A colloidal gold test strip for bovine hydatid antibody involved in the present invention is not limited to the structures described in the following implementation modes. All other implementation modes obtained by those of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0023] Refer to Figures 1 to 5 , the present invention provides a colloidal gold test strip for bovine hydatid antibody, including a detection card housing 1. An embedded device 2 is movably connected inside the detection card housing 1. A fixed buckle 101 is movably connected to the top of the detection card housing 1. A sampling hole 102 is opened at the top of the detection card housing 1. An observation window 103 is opened at the top of the detection card housing 1. A U-shaped limit block 201 is fixedly connected to the bottom of the embedded device 2. A fixed bottom plate 3 is fixedly connected to the top of the embedded device 2. A sample pad 301 is fixedly connected to the top of the fixed bottom plate 3. The detection card housing 1 serves as the main structure of the entire detection card. The detection card housing 1 not only protects the internal components from external contamination and physical damage. The embedded device 2 is the core component inside the detection card. It is combined with the detection card housing 1 through a movable connection. The fixed buckle 101 is located at the top of the detection card housing 1 and is used to fix the embedded device 2 to prevent it from accidentally moving or falling off during the detection process. This design improves the reliability and safety of the detection card. The sampling hole 102 is the entrance for users to add the sample to be detected. Its design allows the sample to quickly and accurately enter the inside of the detection card and react with components such as the sample pad 301. The observation window 103 is located at the top of the detection card housing 1 for facilitating the observation of results. The U-shaped limit block 201 is used to fixedly install the fixed bottom plate 3. The sample pad 301 can absorb and fix the substances to be detected in the sample, providing a basis for subsequent detection reactions.

[0024] Among them, a slide rail 105 is fixedly connected to the inner wall of the detection card housing 1, a guide groove 204 is provided on the side of the embedded device 2, and the side of the slide rail 105 is slidably connected to the embedded device 2. The slide rail 105 is fixedly connected to the inner wall of the detection card housing 1, providing an accurate sliding track for the embedded device 2. The guide groove 204 is provided on the side of the embedded device 2 and closely cooperates with the slide rail 105 on the detection card housing 1, ensuring the smoothness and accuracy of the embedded device 2 during the sliding process and avoiding detection errors caused by shaking or deviation.

[0025] Among them, a hydrophilic film is provided at the bottom of the sample addition hole 102, and an anti-slip pattern 104 is fixedly connected to the top of the detection card housing 1. The hydrophilic film promotes the penetration and distribution of the sample, and the anti-slip pattern 104 prevents the sample from spilling due to accidental slipping of the detection card.

[0026] Among them, a positioning plate 202 is fixedly connected to the top of the embedded device 2, and a limiting groove 203 is fixedly connected to the top of the embedded device 2. The main functions of the positioning plate 202 and the limiting groove 203 are to ensure that the connected fixed bottom plate 3 can be accurately positioned at a predetermined position.

[0027] Among them, a sealing ring 205 is fixedly connected to the top of the embedded device 2, and a limiting protrusion 206 is fixedly connected to the top of the embedded device 2. The main purpose of the sealing ring 205 is to prevent sample leakage between the embedded device 2 and the detection card housing 1.

[0028] Among them, a colloidal gold conjugate pad 302 is fixedly connected to the side of the sample pad 301, a fiber membrane 303 is fixedly connected to the side of the colloidal gold conjugate pad 302, and a chromatographic membrane 304 is fixedly connected to the side of the fiber membrane 303. After the sample pad 301 receives the sample, the sample flows to the colloidal gold conjugate pad 302 by capillary action. On the colloidal gold conjugate pad 302, the analyte in the sample specifically binds to the reagent labeled with colloidal gold, and the bound complex continues to flow to the fiber membrane 303 and the chromatographic membrane 304 for further separation and detection on the chromatographic membrane 304.

[0029] Among them, a quality control line 3031 is provided in parallel at the top of the fiber membrane 303, and a detection line 3032 is provided in parallel at the top of the fiber membrane 303. The quality control line 3031 and the detection line 3032 are provided to facilitate reading the detection results.

[0030] Working principle of the present utility model: The test card is composed of a test card housing 1 and an embedded device 2. The housing protects the internal components and is provided with a sample addition hole 102, an observation window 103 and a fixed buckle 101 to ensure convenient and safe operation. The embedded device 2 is the core component, which is firmly connected to the housing through a slide rail 105, facilitating precise operation and preventing movement. A hydrophilic film is configured at the bottom of the sample addition hole 102 to promote sample penetration. The anti-slip pattern 104 design improves the holding stability. A limiting and sealing structure is provided at the top of the embedded device 2 to ensure no leakage during the detection process. The detection process starts with the sample entering the sample pad 301 through the sample addition hole 102. The sample pad 301 absorbs and fixes the substance to be detected. Subsequently, the sample flows towards the colloidal gold conjugate pad 302 under capillary action and binds to the specific antibody labeled with colloidal gold to form an antigen-antibody complex. This complex continues to migrate to the fiber membrane 303. The quality control line 3031 and the test line 3032 arranged in parallel on the fiber membrane 303 are used to verify the effectiveness of the detection system and specifically detect the analyte respectively. The coloring of the quality control line 3031 indicates that the detection process is normal, while the coloring situation on the test line 3032 directly reflects the presence or absence and concentration of the analyte in the sample. Finally, the user observes the coloring results of the quality control line 3031 and the test line 3032 through the observation window 103 to quickly and accurately judge the detection result. The whole detection process is based on the specific binding of antigen and antibody and chromatography separation technology, realizing the efficient and sensitive detection of bovine echinococcus antibodies.

[0031] The above are only the preferred embodiments of the present utility model and are not intended to limit the present utility model. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present utility model shall be included within the protection scope of the present utility model.

Claims

1. A colloidal gold test card for bovine hydatid antibody, comprising a test card housing (1), characterized in that: The interior of the detection card housing (1) is movably connected to an embedded device (2); the top of the detection card housing (1) is movably connected to a fixed buckle (101); the top of the detection card housing (1) is provided with a sample loading hole (102); the top of the detection card housing (1) is provided with an observation window (103); the bottom of the embedded device (2) is fixedly connected to a U-shaped limit block (201); the top of the embedded device (2) is fixedly connected to a fixed bottom plate (3); and the top of the fixed bottom plate (3) is fixedly connected to a sample pad (301).

2. A colloidal gold test card for bovine hydatid antibody according to claim 1, characterized in that: The inner wall of the detection card housing (1) is fixedly connected to a slide rail (105), the side of the embedded device (2) is provided with a guide rail groove (204), and the side of the slide rail (105) is slidably connected to the embedded device (2).

3. A colloidal gold test card for bovine hydatid antibody according to claim 2, characterized in that: A hydrophilic film is provided at the bottom of the sample addition hole (102), and an anti-slip pattern (104) is fixedly connected to the top of the detection card housing (1).

4. A colloidal gold test card for bovine hydatid antibody according to claim 1, characterized in that: The top of the embedded device (2) is fixedly connected to a positioning plate (202), and the top of the embedded device (2) is fixedly connected to a limiting groove (203).

5. A colloidal gold test card for bovine hydatid antibody according to claim 4, characterized in that: A sealing ring (205) is fixedly connected to the top of the embedded device (2), and a limiting protrusion (206) is fixedly connected to the top of the embedded device (2).

6. A colloidal gold test card for bovine hydatid antibody according to claim 1, characterized in that: The side of the sample pad (301) is fixedly connected to a colloidal gold binding pad (302), the side of the colloidal gold binding pad (302) is fixedly connected to a fiber membrane (303), and the side of the fiber membrane (303) is fixedly connected to a chromatography membrane (304).

7. A colloidal gold test card for bovine hydatid antibody according to claim 6, characterized in that: A quality control line (3031) is opened in parallel to the top of the fiber membrane (303), and a detection line (3032) is opened in parallel to the top of the fiber membrane (303).

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