Inductive pluripotent stem cell cloning selection needle
By designing the needle body with a curved needle body and an acute angle, combining stainless steel material and syringe suction, the problem of low cloning accuracy is solved, and the success rate of cloning and experimental efficiency is improved.
Patent Information
- Application Number
- CN202421931685.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-09
- Publication Date
- 2025-07-04
- Estimated Expiration
- 2034-08-09
AI Technical Summary
In the prior art, the accuracy of selection cloning is not high, resulting in low efficiency.
A needle body including a straight first needle body, a curved needle body, a straight second needle body and a needle tip is designed. By setting the acute angle between the curved needle body and the first needle body, and using stainless steel material, combined with the suction force of the syringe, the effect of precise selection and cloning is achieved.
It improves the success rate and experimental efficiency of cloning, reduces the difficulty of manipulation, and enhances the accuracy of cloning selection.
Smart Images
Figure CN223060976U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of medical devices, in particular to an inducible pluripotent stem cell cloning and picking needle head. Background Art
[0002] The research of iPS cells is currently popular. At present, the method of reprogramming somatic cells into pluripotent stem cells is very inefficient. Generating iPS cell clones is a very time-consuming process, and most of the induced cells are heterologous cells. Even using mature methods, still no more than 1 / 100 of the cells can be transformed into iPS cells. Therefore, during the process of reprogramming somatic cells into iPS cells, a large amount of work is required to select clones, and it is extremely difficult to select clones. It is necessary to clearly identify the target object for separation and not be contaminated by any adjacent clones. The separation and transfer process also needs to be as gentle as possible to avoid unexpected changes or differentiation of cells.
[0003] In current biotechnology laboratories, the selection of clones is a delicate and crucial operation. In the prior art, the conventional method for selecting clones is to scrape them with a 200 μl or 1000 μl pipette tip. After scraping, the clone is directly aspirated through the pipette into another petri dish for continued culture. However, due to the shape and material limitations of the pipette tip, it is very difficult to separate the clone cleanly, and the separated clone cannot be aspirated immediately. At the same time, due to the drift of the clone, it may not be the target clone that is aspirated, resulting in low precision and low efficiency in selecting clones.
[0004] In the prior art, the needle head is straight. When technicians pick clones, they need to push the needle head, which will cause the clone to move forward along the water flow direction, resulting in low efficiency in selecting clones. Content of the Utility Model
[0005] This application provides an inducible pluripotent stem cell cloning and picking needle head to solve the technical problem of low efficiency caused by low precision in selecting clones.
[0006] The inducible pluripotent stem cell cloning and picking needle head provided by this application includes: a needle head body; the needle head body includes a straight first needle body, a curved needle body, a straight second needle body, and a needle tip; one end of the first needle body is used to connect with a syringe barrel, and the other end of the first needle body is connected to one end of the curved needle body; the other end of the curved needle body is connected to one end of the second needle body; the other end of the second needle body is connected to the needle tip; the included angle between the second needle body and the first needle body is an acute angle.
[0007] Preferably, the included angle between the second needle body and the first needle body is denoted as A, and 1° ≤ A ≤ 45°.
[0008] Preferably, the length of the second needle body is denoted as B, and the length of the needle tip is C, and 1 cm ≤ B + C ≤ 3 cm.
[0009] By adopting the above technical solution, the needle body is arranged on the syringe barrel, and a bent needle body is arranged on the needle body, so that the second needle body and the needle tip are bent at a certain angle relative to the first needle body. The bent needle reduces the operation difficulty of the device. At the same time, with the suction of the syringe barrel, the success rate of clone aspiration is improved, the effect of precisely selecting clones is achieved, and the experimental efficiency is improved.
[0010] Preferably, the first needle body, the bent needle body, the second needle body and the needle tip are integrally formed, and the first needle body, the bent needle body, the second needle body and the needle tip are all made of stainless steel.
[0011] By adopting the above technical solution, the integrally formed stainless steel needle body is convenient for production and manufacturing and durable.
[0012] Preferably, a bevel is provided at the opening of the needle tip.
[0013] Preferably, the shape of the bevel at the opening of the needle tip is triangular.
[0014] Preferably, the shape of the bevel at the opening of the needle tip is trapezoidal.
[0015] Preferably, the shape of the bevel at the opening of the needle tip is oval.
[0016] By adopting the above technical solution, the shape of the needle tip is set to be abnormal. Compared with the ordinary pointed needle, the abnormal-shaped needle tip is easier to suck up the clone.
[0017] One or more technical solutions provided in this application have at least the following technical effects or advantages:
[0018] 1. The needle body made of stainless steel is arranged on the syringe barrel, and a bent needle body is arranged on the needle body, so that the second needle body and the needle tip are bent at a certain angle relative to the first needle body. By means of the backward raking of the bent needle, the operation difficulty of clone selection is reduced. At the same time, it is easier to utilize the suction of the syringe barrel by the backward raking method, the success rate of clone aspiration is improved, the effect of precisely selecting clones is achieved, and the experimental efficiency is improved;
[0019] 2. The shape of the needle tip is set to be triangular, trapezoidal or oval. Compared with the ordinary pointed needle, the contact area between the needle tip and the clone is increased, and the abnormal-shaped needle tip with a larger area is easier to suck up the clone. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.
[0021] Figure 1 Isometric view of the needle for picking induced pluripotent stem cell clones provided by this application;
[0022] Figure 2 Front view of the needle for picking induced pluripotent stem cell clones provided by this application;
[0023] Figure 3 One of the isometric views of the tip of the needle for picking induced pluripotent stem cell clones provided by this application;
[0024] Figure 4 Another isometric view of the tip of the needle for picking induced pluripotent stem cell clones provided by this application;
[0025] Figure 5 The third isometric view of the tip of the needle for picking induced pluripotent stem cell clones provided by this application.
[0026] Explanation of reference numerals: 1. Needle body; 11. First needle body; 12. Bent needle body; 13. Second needle body; 14. Tip of the needle; 2. Syringe barrel; 21. Pusher; A. Included angle; B. Length of the second needle body; C. Length of the tip of the needle. Detailed implementation manners
[0027] This application provides a needle for picking induced pluripotent stem cell clones, which is used to solve the technical problem of low efficiency caused by low precision in picking clones in the prior art.
[0028] Next, the technical solutions in the embodiments of this application will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of this application. Obviously, the described embodiments are only a part of the embodiments of this application, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments in this application without creative efforts shall fall within the scope of protection of this application.
[0029] It should be noted that the terms "first", "second", etc. in the specification and claims of this application and the above-mentioned drawings are used to distinguish similar objects, and do not necessarily need to describe a specific order or sequence. It should be understood that such data can be interchanged under appropriate circumstances so that the embodiments of this application described here can be implemented in an order other than those illustrated or described here. In addition, the terms "include" and "have" and any variations thereof are intended to cover non-exclusive inclusion. For example, a process, method, system, product or server that includes a series of steps or units does not necessarily need to be limited to those steps or units clearly listed, but may include other steps or modules that are not clearly listed or are inherent to these processes, methods, products or devices.
[0030] Example 1
[0031] As Figures 1 to 2 shown, an embodiment of the present application provides an induced pluripotent stem cell cloning selection needle, including: a needle body 1; the needle body 1 includes a straight first needle body 11, a curved needle body 12, a straight second needle body 13, and a needle tip 14; one end of the first needle body 11 is used to connect with a syringe 2, and the other end of the first needle body 11 is connected to one end of the curved needle body 12; the other end of the curved needle body 12 is connected to one end of the second needle body 13; the other end of the second needle body 13 is connected to the needle tip 14; the included angle between the second needle body 13 and the first needle body 11 is an acute angle.
[0032] More preferably, in the embodiment provided by the present application, the needle body 1 is installed on a 1 mL syringe 2. The needle body 1 is made of stainless steel and integrally formed. Structurally, the needle body 1 is divided into four sequentially connected parts: the first needle body 11, the curved needle body 12, the second needle body 13, and the needle tip 14. One end of the first needle body 11 is connected to the syringe 2. The curved needle body 12 bends towards the syringe 2. One end of the second needle body 13 with the needle tip 14 is close to the syringe 2. The needle tip 14 adopts a common pointed needle. In this embodiment, the bending radian of the curved needle body 12 is 30°, that is, the included angle A between the second needle body 13 and the first needle body 11 is 30°. The length B of the second needle body 13 is 2 cm, and the length C of the needle tip 14 is 1 cm. That is, the distance from the needle tip 14 to the curved needle body 12 is 3 cm, which can also be understood as the needle body 1 bends at a distance of three centimeters from the needle tip 14.
[0033] During the use process, when using the curved needle, the method of raking the clone backward is adopted. During the raking process, the water flows backward, and the clone naturally falls into the needle. Compared with the pushing and selecting method of a straight needle, the curved needle has higher efficiency during the use process. At the same time, the method of the curved needle raking backward is easier to pull the syringe to utilize the suction of the syringe. Among them, by pulling the push rod 21 of the syringe 2 to drive the piston, the internal volume of the syringe 2 increases to suck in the clone, and by pushing the push rod 21 of the syringe 2 to drive the piston, the internal volume of the syringe 2 decreases to discharge the clone.
[0034] In this embodiment, by setting the stainless steel needle body 1 on the syringe 2 and setting a curved needle body 12 on the needle body 1, the second needle body 13 and the needle tip 14 are bent at a certain angle relative to the first needle body 11. The method of raking backward with the curved needle reduces the operation difficulty of clone selection. At the same time, the method of raking backward is easier to utilize the suction of the syringe 2, improves the success rate of clone suction, achieves the effect of accurately selecting clones, and improves the efficiency of the experiment.
[0035] Example 2
[0036] Further, based on Example 1 with slight differences, the shape of the tip 14 is irregular, such as Figure 3 As shown, in this embodiment, the shape of the tip 14 is triangular. The triangular tip 14 has a larger contact area with the clone compared to a common pointed tip, which can improve the success rate of clone selection and reduce damage to the clone.
[0037] In this embodiment, by setting the shape of the tip 14 to triangular, the contact area at the tip is increased compared to a common pointed needle, making it easier for the triangular tip 14 to pick up the clone.
[0038] Example 3
[0039] Further, based on Example 1 with slight differences, the shape of the tip 14 is irregular, such as Figure 4 As shown, in this embodiment, the shape of the tip 14 is trapezoidal. The trapezoidal tip 14 has a larger contact area with the clone compared to a common pointed tip, which can improve the success rate of clone selection and reduce damage to the clone.
[0040] In this embodiment, by setting the shape of the tip 14 to trapezoidal, the contact area at the tip is increased compared to a common pointed needle, making it easier for the trapezoidal tip 14 to pick up the clone.
[0041] Example 4
[0042] Further, based on Example 1 with slight differences, the shape of the tip 14 is irregular, such as Figure 5 As shown, in this embodiment, the shape of the tip 14 is oval. The oval tip 14 has a larger contact area with the clone compared to a common pointed tip, which can improve the success rate of clone selection and reduce damage to the clone.
[0043] In this embodiment, by setting the shape of the tip 14 to oval, the contact area at the tip is increased compared to a common pointed needle, making it easier for the oval tip 14 to pick up the clone.
[0044] Among them, the shape of the tip 14 is not limited to the triangle, trapezoid, and oval mentioned in the above embodiments.
[0045] It should be noted that the above order of the embodiments of the present application is only for description and does not represent the superiority or inferiority of the embodiments. Also, the above specific embodiments of this specification have been described. Other embodiments are within the scope of the appended claims. In some cases, the actions or steps recited in the claims may be performed in a different order than in the embodiments and still achieve the desired result. Additionally, the processes depicted in the drawings do not necessarily require the particular order or sequential order shown to achieve the desired result. In certain embodiments, multitasking and parallel processing are also possible or may be advantageous.
[0046] The above are only the preferred embodiments of the present application and are not intended to limit the present application. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present application shall be included within the protection scope of the present application.
[0047] This specification and the drawings are only exemplary descriptions of the present application and are considered to have covered any and all modifications, variations, combinations, or equivalents within the scope of the present application. Obviously, those skilled in the art can make various changes and modifications to the present application without departing from the scope of the present application. Thus, if these modifications and variations of the present application fall within the scope of the present application and its equivalent technologies, the present application is intended to include these changes and modifications.
Claims
1. An induced pluripotent stem cell cloning and picking needle, characterized in that: It includes a needle body (1); the needle body (1) includes a straight first needle body (11), a bent needle body (12), a straight second needle body (13) and a needle tip (14); one end of the first needle body (11) is used for connecting with a syringe barrel (2), and the other end of the first needle body (11) is connected with one end of the bent needle body (12); the other end of the bent needle body (12) is connected with one end of the second needle body (13); the other end of the second needle body (13) is connected with the needle tip (14); the included angle between the second needle body (13) and the first needle body (11) is an acute angle.
2. The induced pluripotent stem cell cloning and picking needle according to claim 1, wherein The included angle between the second needle body (13) and the first needle body (11) is denoted as A, and 1° ≤ A ≤ 45°.
3. The induced pluripotent stem cell cloning and selection needle according to claim 2, wherein The length of the second needle body (13) is denoted as B, and the length of the needle tip (14) is C, and 1 cm ≤ B + C ≤ 3 cm.
4. The induced pluripotent stem cell cloning and selection needle according to claim 3, characterized in that, The first needle body (11), the bent needle body (12), the second needle body (13) and the needle tip (14) are integrally formed.
5. The induced pluripotent stem cell cloning and picking needle according to claim 4, wherein The first needle body (11), the bent needle body (12), the second needle body (13) and the needle tip (14) are all made of stainless steel.
6. The induced pluripotent stem cell cloning and selection needle according to claim 5, wherein, A bevel is provided at the opening of the needle tip (14).
7. The needle for induced pluripotent stem cell cloning selection according to claim 6, wherein The shape of the bevel at the opening of the needle tip (14) is triangular.
8. The induced pluripotent stem cell cloning and picking needle according to claim 6, characterized in that, The shape of the bevel at the opening of the needle tip (14) is trapezoidal.
9. The induced pluripotent stem cell cloning and selection needle according to claim 6, wherein, The shape of the bevel at the opening of the needle tip (14) is oval.